ライフサイエンスコーパス: recombinant protein

1 oli to facilitate the functional analysis of recombinant protein.

2 on a post-translational modification of the recombinant protein.

3 tokine and boosted intravaginally with gp140 recombinant protein.

4 al delivery or through systemic injection of recombinant protein.

5 ranslation system to create a Sep-containing recombinant protein.

6 ompletely abolished the GPAT activity of the recombinant protein.

7 tinal epithelial cells by using an Fc-tagged recombinant protein.

8 th the Ser596-containing parasite-derived or recombinant protein.

9 erogeneity present on the IL9 portion of the recombinant protein.

10 es subsequent copurification by a tag on the recombinant protein.

11 s using an affinity resin that binds tagged, recombinant proteins.

12 dehyde product as a bioconjugation handle in recombinant proteins.

13 r lipid bilayer membranes reconstituted with recombinant proteins.

14 ns improves protein synthesis efficiency for recombinant proteins.

15 line (Val) and norvaline (Nva) are common in recombinant proteins.

16 ch was confirmed by coimmunoprecipitation of recombinant proteins.

17 the affinity chromatography purification of recombinant proteins.

18 for manufacturing of synthetic peptides and recombinant proteins.

19 authentic posttranslational modifications in recombinant proteins.

20 oot proteins, using either small peptides or recombinant proteins.

21 exameric archaeal MCM in vitro with purified recombinant proteins.

22 only cleave RNA, at least when assayed with recombinant proteins.

23 cosylation also depends on the nature of the recombinant proteins.

24 ins using rabbits antisera to the respective recombinant proteins.

25 rs using WNT3A and endothelin-1 (EDN1) human recombinant proteins.

26 gulin 1 (NRG1) and nerve growth factor (NGF) recombinant proteins.

27 ried out, both in host cells and on purified recombinant proteins.

28 te that affords purification of FLAG-derived recombinant proteins.

29 research and commercial-scale production of recombinant proteins.

30 sing their specific-inhibitors and exogenous recombinant-proteins.

31 erexpression or local delivery of human CTGF recombinant protein accelerated bridging and functional

32 ransgenic mouse brain and in vitro assembled recombinant protein account for the greater seeding pote

33 to our algal expression strains give rise to recombinant protein accumulation levels of up to 0.25% o

34 ulatory protein alpha (SIRPalpha) knockdown, recombinant proteins, adhesion and fusion assays, biolay

35 We demonstrate that immunization with a recombinant protein Ag and GLA-SE also induces granzyme

36 ng molecular linkers to create a multivalent recombinant protein against Candida albicans (mvPC).

37 ns, and validated by testing the activity of recombinant proteins against hKir6.2.

38 g in vitro biochemical assays with purified, recombinant protein, along with experiments in the adeno

39 hatidic acid-producing kinase activity, DGK4 recombinant protein also revealed guanylyl cyclase activ

40 , share the same enzymatic properties as the recombinant protein and cleaves RNA with inosine but not

41 ith other immunization strategies, including recombinant protein and DNA.

42 al structure with solution state analysis of recombinant protein and electron cryo-microscopy of acid

43 )N labeled AbetaM01-42 fibrils in vitro from recombinant protein and examined their (13)C-(13)C and (

44 We use cross-linking mass spectrometry, recombinant protein and synthetic peptide systems, in si

45 the ssRNA/dsRNA binding sites of a purified recombinant protein and the genomic dsRNA binding sites

46 Using recombinant proteins and a sensitive protein interaction

47 Making use of recombinant proteins and a variety of in vitro assays, w

48 g the outer layer with fluorescently tagged, recombinant proteins and also tagging the DLP, we distin

49 source resource, in the form of high-quality recombinant proteins and antibodies, will accelerate and

50 employed to study the effects of oxidants on recombinant proteins and cell and tissue extracts, and t

51 This includes the preparation and testing of recombinant proteins and DNA templates, clustering DNA t

52 es the selection of two Borrelia burgdorferi recombinant proteins and evaluation of their performance

53 ties in production of site-directed mutants, recombinant proteins and exogenous protein overexpressio

54 n optimization strategies, for production of recombinant proteins and gene therapies.

55 Here, using recombinant proteins and metal transport and cysteine ac

56 body profiling and a panel of conserved EEHV recombinant proteins and proteins unique to EEHV1.

57 Using recombinant proteins and surface plasmon resonance-based

58 in VI by exploring the kinetic properties of recombinant proteins and their distribution in mammalian

59 Overall, antibody reactivity to VAR2CSA recombinant proteins and to CSA-binding IE was higher in

60 Using recombinant proteins and transgenic parasites, we show t

61 stone pre-mRNA processing machinery using 13 recombinant proteins and two RNAs and determined its str

62 Candidate antigens were expressed as recombinant proteins and used to analyze the established

63 pyCatcher (-sc; encoded at the C'-termini of recombinant proteins) and SpyTag.

64 ude mixtures including egg white, unpurified recombinant protein, and a biotransformation supernatant

65 mentation analysis, coimmunoprecipitation of recombinant proteins, and bioinformatic analysis reveale

66 letion, biochemical assays with purified and recombinant proteins, and site-directed mutagenesis, we

67 The recombinant protein anti-EGFR-iRGD also exhibited antitu

68 doids and demonstrate that the corresponding recombinant proteins are also capable of using 8-oxogera

69 Codon usage plays a crucial role when recombinant proteins are expressed in different organism

70 logenetic studies, transport assays with the recombinant protein as well as GFP-based targeting analy

71 ng HP14 zymogen (proHP14), betaGRP2, and the recombinant proteins as truncated forms showed that the

72 ants and the double-variant (V256I-R525W) as recombinant proteins, as well as a less common variant E

73 Recombinant protein assays and complementation studies u

74 and -assembly strategy based on a multiphase recombinant protein based material.

75 Finally, analysis of cell and recombinant protein-based assays indicate that Fer1l6 co

76 Naturally occurring and recombinant protein-based materials are frequently emplo

77 e a pathway for the development of a simple, recombinant protein-based prophylactic vaccine for HCV w

78 y in the 1970s, followed by the invention of recombinant protein-based therapeutic agents in the 1980

79 ne (4CMenB) is an outer membrane vesicle and recombinant protein-based vaccine licensed to protect ag

80 ningococcal group B vaccine 4CMenB is a new, recombinant protein-based vaccine that is licensed to pr

81 zone High-Dose (egg-based, n = 16), Flublok (recombinant protein-based, n = 23), or Flucelvax (cell-b

82 luzone High-Dose (egg-based, n=16), Flublok (recombinant protein-based, n=23), or Flucelvax (cell-bas

83 Here, a bioactive, recombinant, protein-based polymer, termed ZT(Fn) , is p

84 aim of assessing the reliability of current recombinant-protein-based data.

85 and behavior of endogenous and/or non-tagged recombinant proteins be analyzed and altered within the

86 Here we show, that the small recombinant protein binders (PBs) can be used for this p

87 ial for not only enhancing the production of recombinant proteins, but also to treat conformational d

88 semisynthesis that involves the reaction of recombinant protein C-terminal thioesters with N-termina

89 ranspeptidation reaction is used to generate recombinant proteins C-terminally tethered to gels throu

90 The final method using recombinant protein calibrators and stable isotope label

91 Although monomeric MAVS recombinant protein can assemble into prion-like filamen

92 we report the development of a new class of recombinant protein cancer vaccines that deliver differe

93 t NPQ induction inC. reinhardtii Analysis of recombinant proteins carrying the same mutations refolde

94 Here, using purified recombinant proteins, coimmunoprecipitation and HDAC ass

95 The 1.07 MDa recombinant protein complex has histone-exchange activit

96 s in Escherichia coli and enzyme assays with recombinant proteins confirmed that At5g32470 and its ma

97 Here, in vitro cleavage assays with recombinant proteins confirmed that caspase-11 prefers c

98 onjugating gold nanoparticles (Au NPs) and a recombinant protein containing Renilla luciferase (pRluc

99 Here we investigate these issues using a recombinant protein corresponding to the N-terminal coil

100 re, we evaluated the immunogenicity of novel recombinant proteins corresponding to each of the three

101 Recombinant proteins corresponding to particular subdoma

102 While the entire panel of recombinant proteins could be obtained as soluble, folde

103 Binding studies using recombinant proteins demonstrate that SFPQ and NONO asso

104 Binding assays using purified recombinant proteins demonstrate that the interaction be

105 Acetyltransferase activity assays of recombinant proteins demonstrated that a number of group

106 Studies using recombinant proteins demonstrated that SepA is able to c

107 This system leverages the customizability of recombinant protein design to target the conserved recep

108 The recombinant proteins, designated BiFae1A and BiFae1B, we

109 The PlGF mAb promotes (but recombinant protein disrupts) EC barrier function, thus

110 in E. coli (which lacks H(4)B) and that the recombinant protein does not confer growth advantages on

111 The recombinant protein (Ebp1p-6h) can now be produced by a

112 A recombinant protein encoded by B. burgdorferi BB0723 (a

113 pressed in Escherichia coli and rendered the recombinant protein enzymatically active.

114 and immunofluorescence staining, as well as recombinant proteins, ERCC Spike-Ins, and population lys

115 that US9 can direct and control cleavage of recombinant proteins exposed on the luminal leaflet of t

116 fied a minimal core human Ino80 complex from recombinant protein expressed in insect cells.

117 s of endophyte-infected plant tissue and the recombinant protein expressed in the yeast Pichia pastor

118 Here, using recombinant protein expression and in vitro enzyme assay

119 In many cases, large-scale recombinant protein expression is either unfeasible or i

120 in different target classes using different recombinant protein expression systems, which were then

121 We examined MpeV in RS9916 using recombinant protein expression, absorbance spectroscopy,

122 Here, using recombinant protein expression, along with light-scatter

123 lines and primary cortical neuron cultures, recombinant protein expression, immunoprecipitation and

124 emical and biophysical approaches, including recombinant protein expression, LC-MS/MS and 2DE analyse

125 noacyl-tRNA depletion due to a high level of recombinant protein expression, ribosome drop-off can su

126 Here, using recombinant protein expression, site-directed mutagenesi

127 odon usage is one of the factors influencing recombinant protein expression.

128 ality was generated by molecular cloning and recombinant protein expression.

129 malian cells and eventually help to optimize recombinant protein expression.

130 ding of malarial actin has instead relied on recombinant protein extracted and purified from heterolo

131 ffinity chromatography (IMAC) used to purify recombinant proteins features a resin-bound 1:1 Ni(II)-i

132 In vitro analysis of recombinant proteins FHL-1(R127H), FHL-1(A415f/s), and F

133 ase activities on C and mC by using purified recombinant proteins for 11 known human APOBEC proteins

134 The demand for recombinant proteins for analytic and therapeutic purpos

135 s been widely used to produce many different recombinant proteins for basic research and is being use

136 Results obtained with recombinant proteins for both the hemagglutinin and neur

137 asing demand for biotech-based production of recombinant proteins for use as pharmaceuticals in the f

138 (synthetic peptides, QconCAT constructs, and recombinant proteins) for quantitative accuracy, precisi

139 ed two vaccine platforms-mRNA vaccines and a recombinant protein formulated with an MF59-like adjuvan

140 n, was introduced in a bacterially expressed recombinant protein fragment and biophysically character

141 In addition to the above, using recombinant protein fragments, we were able to localize

142 nic region was defined by Western blot using recombinant protein fragments.

143 protein from inclusion bodies and denatured recombinant protein from E. coli membranes.

144 iously cloned the taqIIRM gene, purified the recombinant protein from Escherichia coli, and showed th

145 stigated a vaccine formulation consisting of recombinant proteins from a surface-localized heme trans

146 his strategy could improve the expression of recombinant proteins from anaerobic origin or those with

147 We generated recombinant proteins from the mouse cDNAs encoding the H

148 ed by glutaraldehyde, and ii) the His-tagged recombinant protein G.

149 laque, was further investigated by using the recombinant protein generated from E. coli containing a

150 The use of recombinant proteins has revolutionized the development

151 Single-molecule methods using recombinant proteins have generated transformative hypot

152 Several recombinant proteins have potential to mediate induction

153 and their expression as biologically active recombinant proteins have provided a basis for studying

154 changes rather than affinity for individual recombinant proteins, have recently attracted renewed in

155 ng production of tyrosine and secretion of a recombinant protein in Saccharomyces cerevisiae by up to

156 Interestingly, when we examined CPEB3 recombinant protein in vitro, we found that CPEB3 phase

157 formance of this assay by first using spiked recombinant proteins in a complex media (FBS) and measur

158 , viral antigens were introduced as purified recombinant proteins in adjuvant as native proteins.

159 Overexpression of recombinant proteins in bacteria may lead to their aggre

160 ucted for the rapid and stable expression of recombinant proteins in different plant species, allowin

161 onobacter racemifer-as soluble heterodimeric recombinant proteins in E. coli for the first time.

162 minant B. microti antigens were expressed as recombinant proteins in E. coli.

163 The production of recombinant proteins in Escherichia coli frequently resu

164 st cell extracts as well as when produced as recombinant proteins in Escherichia coli.

165 ProRS isoforms were expressed as His-tagged recombinant proteins in Escherichia coli.

166 activity, we performed in vitro assays with recombinant proteins in HEK293T cells and ex vivo assays

167 post-translational, covalent conjugation of recombinant proteins in plants.

168 enable large-scale production of high-value recombinant proteins in sugarcane culms.

169 bacterial production systems accumulate the recombinant proteins in the intracellular space, which r

170 r protease inhibitory mAbs by coexpressing 3 recombinant proteins in the periplasmic space of Escheri

171 ssed five P. vivax spleen-dependent genes as recombinant proteins, including members of three differe

172 4CMenB contains 3 main recombinant proteins, including the Neisseria meningitid

173 Delivery of native or chemically modified recombinant proteins into mammalian cells shows promise

174 ted to detect any other protein if a His-tag recombinant protein is available.

175 lassical enzyme replacement therapy with the recombinant protein is not possible as the NPC1 gene pro

176 Moreover, we found that the recombinant protein is stable at both frozen and elevate

177 f multiple DNA plasmids, messenger RNAs, and recombinant proteins is developed to allow high spatiote

178 However, the use of cell culture to produce recombinant proteins is still susceptible to contaminati

179 nergy transfer assay against the full-length recombinant protein kinase PfCLK3, and 11 analogues were

180 approaches, gene-knockout mutants, purified recombinant proteins, LC-MS-based analyses of O-glycans,

181 coding retrovirus and in organ culture using recombinant protein, led to intestinal aganglionosis.

182 osoma mansoni infections, we have compiled a recombinant protein library of parasite cell-surface and

183 enB) vaccine is the first vaccine containing recombinant proteins licensed for the prevention of inva

184 trong correlation among positive serology to recombinant proteins LinB-13, 26, 15, 21 and to salivary

185 ng proteins were confirmed using co-IP using recombinant proteins, liver lysates, and mitochondria.

186 mal and GAGs-deficient cells showed that the recombinant proteins maintain their GAG-targeting activi

187 Using site-directed mutagenesis, purified recombinant proteins, mammalian cell expression, and flu

188 such a manner that the humoral response to a recombinant protein may be enhanced by coadministering w

189 sorders and for other hepatic diseases where recombinant proteins may be unaffordable or unsuitable.

190 During the production of recombinant proteins, misincorporation of Nva (norvaline

191 e versatility of this platform in monitoring recombinant protein modification of peptide substrates,

192 y the development of novel functions such as recombinant proteins modified to have different function

193 flagellin knock-out derivative and purified recombinant protein, MRx0518 flagellin was shown to be a

194 From sucrose alone, the corresponding recombinant protein, named BRS-B, mainly catalyzed sucro

195 Two recombinant proteins, NF2152 and NF2523, were characteri

196 tive in quenching in vivo, implying that the recombinant protein obtained is a good material for futu

197 d complementation, we constructed a panel of recombinant proteins of 1 to 3 subunits that contained c

198 In sharp contrast, recombinant proteins of the N-terminal region of MUC5B (

199 We have reconstituted active C/D RNPs with recombinant proteins of the thermophilic yeast Chaetomiu

200 Among cargoes, recombinant proteins offer therapeutic promise but the l

201 Hydrophobic recombinant proteins often tend to aggregate upon expres

202 istration of angiogenic cytokines, either as recombinant protein or as gene therapy, and more recentl

203 (but not to toxoids), whether raised to the recombinant protein or to TcdA26-39 expressed on the B.

204 were validated by enzymatic assays with the recombinant proteins or by their complementation of yeas

205 Treatment of Ocy454-Gsalpha(cont) cells with recombinant proteins or knockdown of Nrp-1 and Grn in Oc

206 mpounds, so-called biologicals (eg, mAbs and recombinant proteins), or by a rather new class of molec

207 Making use of VAR2CSA recombinant proteins originating from both species, we s

208 rted a Plasmodium yoelii chimeric multistage recombinant protein (P. yoelii linear peptide chimera/re

209 by enzymatic assays and mass spectroscopy on recombinant protein, patient-derived fibroblasts, plasma

210 The Arg-degrading recombinant protein, pegylated arginine deiminase (ADI-P

211 f a clickable group at the C terminus of the recombinant protein/peptide with almost complete convers

212 (MC-LR) biosensor based on the inhibition of recombinant protein phosphate type 1 (PP1alpha) is repor

213 h as examples and showed that these purified recombinant proteins possessed AKR activity using variou

214 Interestingly, the recombinant protein produced from the ADH1B2 variant, co

215 ollowing agroinfiltration or infiltration of recombinant proteins produced by Pichia pastoris.

216 Furthermore, using recombinant proteins produced in E. coli, we show that t

217 ber of E. coli expression systems for use in recombinant protein production and represents a major pe

218 workflow should enable future research into recombinant protein production by P. pastoris and a synt

219 Recombinant protein production coopts the host cell mach

220 ulating protein expression levels to improve recombinant protein production has become an important t

221 are a major class of impurities derived from recombinant protein production processes.

222 ysiological and secretory differences across recombinant protein production systems.

223 stabilized cyclopropenones were suitable for recombinant protein production via genetic code expansio

224 SA1), and cell cycle (CDC39), can all impact recombinant protein production when expressed at differe

225 th or secretion from nonhuman cells used for recombinant protein production.

226 on yeast, along with biochemical assays with recombinant proteins, provide evidence that Rbins' physi

227 reported on a single-step method integrating recombinant protein purification with 2-cyanobenzothiazo

228 has the ability to produce a wide variety of recombinant proteins, ranging from simple peptides to co

229 Another recombinant protein rDA27(aa 33-84) that removes the GAG

230 noviral vector and loaded in tandem with the recombinant protein reduced parasite burdens by 76% to >

231 alving the number of expression plasmids and recombinant proteins required for screening.

232 rus serotype 9 (AAV9) gene therapy vector or recombinant protein, resulted in a complete arrest of fo

233 The results using the recombinant proteins revealed that all antigens discrimi

234 Functional characterization of the purified recombinant proteins revealed that NnOMT1 is a regiospec

235 of sow and piglet serum samples on a SVA VP1 recombinant protein (rVP1) indirect enzyme-linked immuno

236 ediated conjugation of a small molecule to a recombinant protein scaffold we pave the way to biomedic

237 ax), and the other is based on adjuvanted gE recombinant protein (Shingrix).

238 ghtforward incorporation of Tyr-Leu-Ala into recombinant proteins should make this system attractive

239 Biochemical experiments with purified recombinant proteins show that SSRP1 and Spt16 are able

240 Rac-GEF activity assays with purified recombinant proteins showed that direct interaction with

241 Binding assays with purified recombinant proteins showed that their interaction is di

242 ent activation products to CC in vitro using recombinant proteins, specific inhibitors, as well as de

243 ans, of a novel chlamydia vaccine based on a recombinant protein subunit (CTH522) in a prime-boost im

244 vaccine, we sought to generate and evaluate recombinant protein subunit vaccines against C. burnetii

245 Additional studies using CXCL5 recombinant protein suggest that CXCL5 is sufficient to

246 Furthermore, functional studies of recombinant protein suggested that the truncated protein

247 The enzyme assays of mixed and coexpressed recombinant proteins supported physical interactions bet

248 d Motif V mutations in both the replicon and recombinant protein systems to investigate viral genome

249 cellular events, we verified by generating a recombinant protein that CXXC5 is indeed an unmethylated

250 ts requirement for the production of soluble recombinant protein that is functional in processive DNA

251 ing high levels of EPA and DHA together with recombinant proteins: the fungal Aspergillus niger PhyA

252 Recombinant protein therapeutics, vaccines, and plasma p

253 erved the characteristic of HLC allowing the recombinant protein to be expressed in Yeast (such as Pi

254 for selective labeling of His-tag containing recombinant proteins to enable their sensitive detection

255 d and screened by phage ELISA using trimeric recombinant proteins to identify viral envelope specific

256 ctional experiments in the cell model and on recombinant proteins to investigate the pathogenic effec

257 Here we used recombinant proteins to reconstitute and assess lysine a

258 to synthesize complex N-glycan structures on recombinant proteins to study their contribution to immu

259 roducts available, ranging from the specific recombinant proteins to treat FVII- and FXIII-deficient

260 ion for surrogate protein calibrators (i.e., recombinant proteins) to produce inaccurate concentratio

261 Activity assays performed using recombinant protein unveiled differences in substrate bi

262 strategy to achieve high-level expression of recombinant proteins using a combinatorial stacked promo

263 ation with SARS-CoV-2 mRNA, but not with the recombinant protein vaccine, elicited potent SARS-CoV-2-

264 orating 2,3-diaminopropionic acid (DAP) into recombinant proteins, via expansion of the genetic code(

265 igation, here we showed that a cysteine in a recombinant protein was chemically activated by a small

266 The recombinant protein was easily purified by heat precipit

267 The recombinant protein was expressed, and its kinetic param

268 The recombinant protein was functional when reconstituted in

269 The recombinant protein was hypothesized to be a CBP enzyme

270 One recombinant protein was identified as a candidate diagno

271 The purified recombinant protein was recognized by rabbit anti-C. irr

272 Using recombinant protein, we demonstrate for the first time t

273 Using purified recombinant protein, we demonstrated that lymphostatin i

274 Using purified recombinant protein, we show that the Leu-Phe substituti

275 Using purified recombinant proteins, we demonstrated that DJ-1 directly

276 Analyzing the recombinant proteins, we found that, similar to SOD5, Cu

277 with site-specific mutagenesis and purified recombinant proteins, we further characterized the Srx-T

278 yte and skin resident cell preparations, and recombinant proteins, we have identified that neutrophil

279 oblotting with CLCA1-specific antibodies and recombinant proteins, we observed that the CLCA1 C-termi

280 g plasmid-length DNA substrates and purified recombinant proteins, we show that the combined exonucle

281 and enzyme-linked immunosorbent assay using recombinant proteins, we verified the direct interaction

282 Inhibitors and recombinant protein were used to determine the upstream

283 of CD31, specific monoclonal antibodies and recombinant proteins were regarded as blocking agents be

284 Recombinant proteins were shown to have high substrate s

285 Recombinant proteins were used to examine nitric oxide (

286 essed in a bacterial system and the purified recombinant proteins were used to for antibodies prepara

287 an thrombin, PDGF-BB, Avidin, and His-tagged recombinant protein, were studied, and the results showe

288 These recombinant proteins, when screened for their ability to

289 in45 and Syntaxin5 in pull-down assays using recombinant proteins, whereas this mutant Golgin45 bindi

290 We have developed small, recombinant proteins which bind a critical junction prot

291 well as M. tuberculosis strains that express recombinant proteins which traffic or fail to traffic to

292 MSA differ from those formed in vitro using recombinant proteins, which has implications for underst

293 the presence of hexa-histidine tags in many recombinant proteins, which have high affinity toward a

294 accine candidates for antibody responses are recombinant proteins, which were not included in the ini

295 n microscopy; compared the properties of the recombinant protein with those of CLEC3A extracted from

296 Immunization of mice with recombinant proteins with a variety of adjuvants induced

297 tical detection now permits the detection of recombinant proteins with fluorescence excitation at 488

298 in-depth biochemical characterization of 17 recombinant proteins with structural biology-based inves

299 sion profiles and in vitro products of their recombinant proteins with volatile terpene profiles from

300 essing biopharmaceutically relevant or other recombinant proteins without the need for any toxic sele