ライフサイエンスコーパス: recombinational

1 ation may feed back to regulate local Dbeta2 recombinational accessibility during thymocyte developme

2 To determine whether Vbeta14 recombinational accessibility is subject to feedback inh

3 sembled TCR beta-chains did not downregulate recombinational accessibility of Vbeta14 chromatin.

4 in part, to the particular pattern of V gene recombinational accessibility that occurs in differentia

5 on of germline promoters is central to V(D)J recombinational accessibility, driving chromatin remodel

6 promoter that directs its transcription and recombinational accessibility.

7 iation and subsequent feedback inhibition of recombinational accessibility.

8 ity and V(H) mutational diversity and led to recombinational activation of allelically excluded IgH g

9 Recombinational activities are also capable of both leng

10 Recombinational activity correlates with DNA binding: Cc

11 uman minisatellites is controlled by intense recombinational activity in DNA flanking the repeat arra

12 een 11q23 and 22q11--but not typical meiotic recombinational activity in meiotic prophase--plays an i

13 There is concern, however, whether extensive recombinational analysis can be very practical in light

14 ies could provide an invaluable tool for the recombinational analysis of factors relevant to pathogen

15 achromosomal telomeric repeat DNAs, putative recombinational byproducts that we show exist as intertw

16 g the gaps can be accelerated by a selective recombinational capture of missing chromosomal segments

17 Using a dual luciferase model adapted for recombinational cloning and use in Toxoplasma gondii, we

18 human ORFeome using a newly improved Gateway recombinational cloning approach.

19 We examine the methods for recombinational cloning available for both the creation

20 ng open reading frames (ORFs), in a flexible recombinational cloning format.

21 The recombinational cloning involved in the Gateway system,

22 are the available commercial and open-source recombinational cloning methods with regard to their use

23 We have integrated recombinational cloning of full-length trimmed ORF clone

24 In conclusion, the use of the alternative recombinational cloning system in yeast may greatly acce

25 Here we discuss the use of a recombinational cloning system that allows efficiency, a

26 We used yeast recombinational cloning to characterize four distinct Ex

27 We have also applied yeast recombinational cloning to facilitate a targeted mutagen

28 The ORFs were captured in a recombinational cloning vector to facilitate high-throug

29 soforms using reverse transcriptase (RT)-PCR recombinational cloning, 'deep-well' pooling and a next-

30 rategy, based on automated primer design and recombinational cloning, allowing one to rapidly go from

31 ockout mutants in which we make use of yeast recombinational cloning, Neurospora mutant strains defic

32 Generated by Gateway recombinational cloning, this collection contains 12,212

33 are genetic restrictions that may influence recombinational compatibility.

34 We show that the recombinational constraints of some HVRs are correlated,

35 s and chimpanzees (8 x 10(-5) and 4 x 10(-7) recombinational deletion events per locus per generation

36 selection, and highlight the consequences of recombinational deletion for dating recent HERV integrat

37 meiotic recombination to the probability of recombinational deletion to explain the effect of recomb

38 by a simulation in which the probability of recombinational deletion was reduced 10-fold by a single

39 either inactivated by mutation or removed by recombinational deletion.

40 ous retroviruses (HERVs) has been to undergo recombinational deletion.

41 cquire inactivating mutations or are lost by recombinational deletion.

42 in DNA complexes, and how site selection and recombinational directionality are determined.

43 compounding risk of virus escape by means of recombinational diversification.

44 recombination of Ab isotype is mediated by a recombinational DNA deletion event and must be robustly

45 Defects in recombinational DNA double strand break repair, Rad6-med

46 tS family protein hMSH4 functions in meiotic recombinational DNA double-strand break (DSB) repair.

47 In recombinational DNA double-strand break repair a homolog

48 RAD51, a key protein in the homologous recombinational DNA repair (HRR) pathway, is the major s

49 The BRCA2 gene is involved in recombinational DNA repair and cytokinesis.

50 t the length of RecA filaments formed during recombinational DNA repair and other activities.

51 t be active for different cellular events in recombinational DNA repair and replication fork stabiliz

52 nclude that this helicase may be involved in recombinational DNA repair and the resumption of replica

53 which the DNA substrates needed to initiate recombinational DNA repair are present.

54 how that it both binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD5

55 Recombinational DNA repair by the RecF pathway of Escher

56 2 and Rad59 function coordinately to enhance recombinational DNA repair either by directing the proce

57 the phosphatase Cdc14 is essential to fulfil recombinational DNA repair in budding yeast.

58 ework for understanding the initial steps of recombinational DNA repair in eukaryotes.

59 sh two of the core machineries that initiate recombinational DNA repair in human cells.

60 est a mechanism to enhance the efficiency of recombinational DNA repair in the context of severe geno

61 RecA is essential to recombinational DNA repair in which RecA filaments media

62 Overall, the results suggest that recombinational DNA repair is a common occurrence in cel

63 leton structure in supporting the homologous recombinational DNA repair machinery and genome integrit

64 ociated protein PALB2 in the assembly of the recombinational DNA repair machinery at DNA damage sites

65 Instead, enhanced recombinational DNA repair makes a prominent but probabl

66 process is essential to efficiently enhance recombinational DNA repair pathways that depend on long-

67 icated in the nucleotide excision repair and recombinational DNA repair pathways.

68 roteins mediate homologous recombination and recombinational DNA repair through homology search and s

69 are important for the optimal functioning of recombinational DNA repair with multiple terminal target

70 The cjj81176_1279 (recR; recombinational DNA repair) and cjj81176_1449 (unknown f

71 We propose that RecA, recombinational DNA repair, and branch migration are all

72 This homology search is vital to recombinational DNA repair, and results in homologous pa

73 component of the BRCA complex important for recombinational DNA repair.

74 ceptibility protein, BRCA2, is essential for recombinational DNA repair.

75 he DNA strand invasion step of RecA-mediated recombinational DNA repair.

76 a role of BLM and Exo1 in the initiation of recombinational DNA repair.

77 hat branch migrate Holliday junctions during recombinational DNA repair.

78 he Rad51 filament, a central intermediate in recombinational DNA repair.

79 modification that occur during the course of recombinational DNA repair.

80 ncy and outcome (crossover/non-crossover) of recombinational DNA repair.

81 damage tolerance and plays a crucial role in recombinational DNA repair.

82 f2 family with a specialized function during recombinational DNA repair.

83 tive nuclease to a constructive component of recombinational DNA repair.

84 tant for both restriction of foreign DNA and recombinational DNA repair.

85 hosphoregulated circuitry for the control of recombinational DNA repair.

86 al role of DinD protein in the regulation of recombinational DNA repair.

87 suppressor protein BRCA2 plays a key role in recombinational DNA repair.

88 yeast cells, so that replication bypass and recombinational DSB repair cannot occur.

89 ise of genome sequencing for elucidating the recombinational dynamics of plant mitochondrial genomes.

90 ry, some researchers have suggested that the recombinational effect of PRDM9 is lineage or species sp

91 gest that these result from futile cycles of recombinational elongation and truncation of the Acc rep

92 ssembly of an invertasome complex in which a recombinational enhancer DNA segment bound by the Fis pr

93 recombinases that are regulated by a remote recombinational enhancer element containing two binding

94 s and requires the Fis regulatory protein, a recombinational enhancer, and a supercoiled DNA substrat

95 The Fis-bound recombinational enhancer, together with topological cons

96 To investigate the effects of this recombinational environment on patterns of nucleotide va

97 inked neutral diversity depends on the local recombinational environment.

98 ferences in the efficacy of selection across recombinational environments.

99 ch divergent regions plausibly originated by recombinational events by which a gene and/or spacers wa

100 he locus and within the individual units, of recombinational events that give rise to the concerted e

101 at SARB strains show evidence for widespread recombinational exchange in contrast to results obtained

102 ections (chiasmata) between homologs through recombinational exchange of chromosome arms after sister

103 inability of these bacteria to commence the recombinational exchange process seems to confer genetic

104 The frequency of recombinational exchanges (FRE) that disrupt co-inherita

105 does not cause the documented differences in recombinational frequency of the left arm of chromosome

106 can take advantage of both the mutation and recombinational histories of the sample.

107 lyses to haplotypes with common mutation and recombinational histories.

108 ting errors in haplotypes by considering the recombinational history implied by the patterns of varia

109 magnitude, implying great differences in the recombinational history of different portions of our gen

110 s on the CTG.CAG sequences, suggest that the recombinational hot spot characteristics may be a common

111 We sought to identify ERV progenitors, recombinational hot spots, and segments that are always

112 trands and digests them until encountering a recombinational hotspot (Chi site).

113 age disequilibrium (LD), but the impact that recombinational hotspots have on sites linked to selecte

114 n Rad9 also resulted in decreased homologous recombinational (HR) repair, which occurs predominantly

115 found that site-specific I-SceI DSBs mediate recombinational IgH locus class switching from IgM to Ig

116 With this high intrinsic recombinational instability, the rDNA clusters may serve

117 inhibition can explain even spacing of total recombinational interactions and implies that establishm

118 Thus, during wild-type meiosis, recombinational interactions are differentiated into CR

119 hin the region and were presumably caused by recombinational interactions between SDs harboring the S

120 tes of crossover-designated, axis-associated recombinational interactions to mediate crossover/chiasm

121 SCP1 shows much evidence of recombinational interactions with other replicons and tr

122 ting double-strand breaks (DSBs) and ensuing recombinational interactions, including foci of the esse

123 balance the disruption and stabilization of recombinational interactions, respectively, to regulate

124 The model reveals a recombinational landscape that is highly enriched in fun

125 e by recombination, which we refer to as the recombinational landscape.

126 ("clonality/sexuality machinery") to escape recombinational load.

127 dancy may prevent vaccine escape variants by recombinational loss, which is frequent in pneumococcus.

128 g chromosomal deletions, as well as standard recombinational mapping on a subset of the mutations.

129 o homeology is a result of the difference in recombinational mechanism and/or the timing of the obser

130 Consistent with a recombinational mechanism of single-strand annealing, cl

131 cer cells achieve immortalization by using a recombinational mechanism termed ALT (alternative length

132 s despite their continued elimination by the recombinational mechanisms of concerted evolution.

133 o the Rad52-dependent nonrecombinational and recombinational modes of PRR.

134 instability in primates via insertional and recombinational mutagenesis.

135 However, we also observed "recombinational neighborhoods," where adjacent intervals

136 ide evidence for the activation of the RAD52 recombinational pathway in the pol30-119 mutant and we i

137 that Sgs1p participates in a RAD52-dependent recombinational pathway of telomere maintenance.

138 be repaired by three pathways: an error-free recombinational pathway requiring NER and HR and two PRR

139 by shunting the reaction through an aberrant recombinational pathway that leads to amplifications and

140 with Srs2, it is not a conventional form of recombinational pathway.

141 deficient in ERCC1-XPF, which is involved in recombinational pathways as well as cross-link repair.

142 ce in genes and spacers identified different recombinational patterns.

143 of the H. pylori gene content and its highly recombinational population structure.

144 recombinant inbred, haploid and double-cross recombinational populations, in addition to conventional

145 isticated regulatory mechanisms on the basic recombinational process to favor one particular outcome

146 Thus, in N. gonorrhoeae, recombinational processes are facilitated by RecX(Ng) pr

147 results raise the hypothesis that changes in recombinational processes, including gene conversion, ma

148 is critical in minimizing the need for both recombinational processing of blocked replication forks

149 ec8 and Red1/Mek1 also independently license recombinational progression and abundantly localize to d

150 sequence homology found in maize influences recombinational properties and local linkage disequilibr

151 The recombinational properties of long GAA.TTC repeating seq

152 on if RecA is not available, suggesting that recombinational rather than regulatory functions of RecA

153 inhibitor, DNA damage, and homology-directed recombinational repair (HDR) in human breast cancer cell

154 R) (rad6, rad18, mms2, and rad5), homologous recombinational repair (HRR) (rad51 and rad54), base exc

155 tra-S-phase checkpoint, decreased homologous recombinational repair (HRR) activity, down-regulated XI

156 Homologous recombinational repair (HRR) of DNA damage is critical f

157 In vertebrates, homologous recombinational repair (HRR) requires RAD51 and five RAD

158 Homologous recombinational repair (HRR) restores chromatid breaks a

159 gmentation, making seqA mutants dependent on recombinational repair (the seqA recA colethality).

160 We suggest that nicks become substrates for recombinational repair after being converted into double

161 d breaks, which are mended by RecA-catalysed recombinational repair and are lethal if not repaired.

162 romatin and nuclear matrix), and enables its recombinational repair and checkpoint functions.

163 ation, cell division, DNA damage monitoring, recombinational repair and detoxification.

164 ght modulate the ability of RAD51 to promote recombinational repair and lead to an increased risk of

165 ir, FANCJ encodes a DNA helicase involved in recombinational repair and replication stress response.

166 of SUMO with its newly identified effects on recombinational repair and stimulate further thought on

167 nction of Mre11 is required for RMX-mediated recombinational repair and telomere stabilization in mit

168 distinct pathways in replication-associated recombinational repair and that the Smc5/6 complex and E

169 cules may be generated from blocked forks by recombinational repair and/or replication fork regressio

170 DNA damage checkpoint and recombinational repair are both important for cell survi

171 amage, but the effects of phosphorylation on recombinational repair are unknown.

172 iety of mutant forms of Brh2 for activity in recombinational repair as measured by DNA repair profici

173 However, the process of recombinational repair between short DNA regions of acci

174 eas DSBs in unique sequences are confined to recombinational repair between the large regions of homo

175 BRCA2 protein regulates recombinational repair by interaction with RAD51 via a s

176 strain, suggesting that increased levels of recombinational repair could account for its increased r

177 s, mismatch repair deficiency and homologous recombinational repair deficiency, as well as mutational

178 NA helicases, and is proposed to function in recombinational repair during DNA replication.

179 We propose a model in which recombinational repair during S phase coupled with failu

180 red following synapsis for completion of the recombinational repair event.

181 ction by RecJ and RecQ permits initiation of recombinational repair from all dsDNA ends: 5'-overhangs

182 a, but not ackA, mutants also depend on late recombinational repair functions RuvABC or RecG.

183 Several studies have revealed that the recombinational repair genes, RAD51 and BRCA1, and the D

184 We propose that the up-regulation of recombinational repair in dam mutants allows for the eff

185 The RecF pathway has many parallels with recombinational repair in eukaryotes.

186 These results suggest that recombinational repair in the context of heterochromatin

187 s, and this activity enhances early steps of recombinational repair in vitro.

188 nto minichromosomes regulates early steps of recombinational repair in vitro.

189 vation of Yen1, likely to resolve persistent recombinational repair intermediates.

190 l fragmentation, which becomes inhibitory if recombinational repair is also inactivated (the rdgB rec

191 Homologous recombinational repair is an essential mechanism for rep

192 m long 3'-ssDNA overhangs in preparation for recombinational repair is catalyzed by the coordinated a

193 In eukaryotes, recombinational repair is choreographed by multiprotein

194 Replication-associated recombinational repair is important for genome duplicati

195 Thus, DNA synthesis associated with recombinational repair must be largely error-free.

196 Recombinational repair normally requires a battery of pr

197 2AX serine 139 enforces efficient homologous recombinational repair of a chromosomal double-strand br

198 RAD51AP1-depleted cells are impaired for the recombinational repair of a DNA double-strand break and

199 Mps3p, and Mps3p-dependent tethering delays recombinational repair of a DSB and enhances gross chrom

200 ular events that occur during the homologous recombinational repair of a programmed double-strand chr

201 ombinase, we also show that Srs2 can aid the recombinational repair of camptothecin-induced collapsed

202 of foreign linear DNA and in RecA-dependent recombinational repair of chromosomal lesions in E. coli

203 During genetic recombination and the recombinational repair of chromosome breaks, DNA molecul

204 Similar to Chk1 and Rad17, which enhance recombinational repair of collapsed replication forks, w

205 ion in multiple cellular processes including recombinational repair of DNA and nuclear export of mess

206 pathway of Escherichia coli is important for recombinational repair of DNA breaks and gaps.

207 formed between sister chromatids during the recombinational repair of DNA breaks or after replicatio

208 n and support two different functions during recombinational repair of DNA breaks.

209 e BRCA2 ortholog in Ustilago maydis, enables recombinational repair of DNA by controlling Rad51 and i

210 CA2 homolog in Ustilago maydis, functions in recombinational repair of DNA damage by regulating Rad51

211 is a protein that regulates RAD51-dependent recombinational repair of DNA double strand breaks (DSB)

212 PALB2 links BRCA1 and BRCA2 in homologous recombinational repair of DNA double strand breaks (DSBs

213 sistent with gene conversion associated with recombinational repair of DNA double-strand breaks.

214 at HDAC enzymes are important for homologous recombinational repair of DNA double-strand breaks.

215 he bacterial RecN protein is involved in the recombinational repair of DNA double-stranded breaks, an

216 Bacterial recombinational repair of double-strand breaks often beg

217 The rdgB mutants depend on recombinational repair of double-strand breaks.

218 Rad54 protein plays an important role in the recombinational repair of double-strand DNA (dsDNA) brea

219 Rad51 protein (Rad51) is central to recombinational repair of double-strand DNA breaks.

220 Rad52 performs multiple functions during the recombinational repair of double-stranded DNA (dsDNA) br

221 Rad51 is a conserved protein essential for recombinational repair of double-stranded DNA breaks (DS

222 cerevisiae Tid1 protein is important for the recombinational repair of double-stranded DNA breaks dur

223 During recombinational repair of double-stranded DNA breaks, RA

224 othesis that filamin-A influences homologous recombinational repair of DSB and the maintenance of gen

225 results in a 2-fold reduction of homologous recombinational repair of DSB.

226 The involvement of hPso4 in the recombinational repair of DSBs provides an explanation f

227 favor interhomolog, rather than intersister recombinational repair of genetically programmed DSBs in

228 is a prerequisite for the timely homologous recombinational repair of meiotic DNA double-strand brea

229 Rad55 and Rad57 have different roles in the recombinational repair of stalled replication forks comp

230 The proteins participate in recombinational repair of stalled replication forks or D

231 y1 cDNA molecules are then used as donors in recombinational repair of the break before it is healed.

232 to the formation of a DNA joint molecule and recombinational repair of the DSB.

233 esis, we propose that TWINKLE is involved in recombinational repair of the human mitochondrial DNA.

234 show that MutS2 plays no role in mismatch or recombinational repair or deletion between direct DNA re

235 -link on one strand (unhooking), followed by recombinational repair or lesion bypass synthesis.

236 ect interaction with PALB2, BRCA1 fine-tunes recombinational repair partly through its modulatory rol

237 germ line, like yeast, employ the homologous recombinational repair pathway more often than imperfect

238 , reinforcing our previous findings that the recombinational repair pathway plays a minor role in M.

239 ally, dut mutants depend on the RecBC-RuvABC recombinational repair pathway that mends double-strand

240 Homologous recombinational repair preserves chromosomal integrity b

241 heless, few genes encoding components of DNA recombinational repair processes have been identified in

242 on strand invasion intermediate in these two recombinational repair processes.

243 PF/ERCC1 is stably associated with hRad52, a recombinational repair protein, in human cell-free extra

244 Recombinational repair provides accurate chromosomal res

245 t high temperature, suggesting dependence on recombinational repair rather than on the RecBCD-catalyz

246 Testing known mutants in recombinational repair revealed an additional interactio

247 ns in additional components of the bacterial recombinational repair system and the replication restar

248 Furthermore, by comparison, its recombinational repair system seems to be only minimally

249 ad51 complex and balances a finely regulated recombinational repair system.

250 s a structure-specific mediator that targets recombinational repair to ssDNA-dsDNA junctions.

251 te by the intron RNP particles, gapping, and recombinational repair using homologous sequences in don

252 n and the up-regulation of genes involved in recombinational repair with the level of DNA damage, we

253 that Rad55-S2,8,14 phosphorylation activates recombinational repair, allowing for faster recovery aft

254 king protein involved in DNA replication and recombinational repair, and it is important for preserva

255 A (ssDNA) are essential for DNA replication, recombinational repair, and maintenance of genomic stabi

256 luding those involved in mismatch repair and recombinational repair, and that is noted for high level

257 nown as the RAD51 paralogs are important for recombinational repair, as paralog-defective cell lines

258 involved in the early stage (presynapsis) of recombinational repair, but it has a RecN homologue with

259 ly conserved Smc5/6 complex is implicated in recombinational repair, but its function in this process

260 compete for Holliday junction structures in recombinational repair, but since a classic RecG resolva

261 on (HJ) processing pathways are required for recombinational repair, each can act during genetic tran

262 otic DNA double-strand breaks (DSBs) undergo recombinational repair, genetic crossovers (COs) may be

263 BRCA1, Rad51, and CHK2 contribute to recombinational repair, in part independently of H2AX.

264 BRE1 and DOT1 is mediated through homologous recombinational repair, not postreplication repair, and

265 epair, nucleotide excision repair (NER), and recombinational repair, plays a critical role in maintai

266 Thus, H. pylori RecN, as a component of DNA recombinational repair, plays a significant role in H. p

267 enes involved in nucleotide excision repair, recombinational repair, postreplication repair including

268 s that MMR sensitization is due to decreased recombinational repair, we used a RecA-mediated strand e

269 Recombinational repair-dependent mutants identify ways t

270 se) that is involved in the major pathway of recombinational repair.

271 n contrast to E. coli, H. pylori RecG limits recombinational repair.

272 activation of the DNA damage checkpoints and recombinational repair.

273 recruited to the HO lesion during homologous recombinational repair.

274 oci, suggesting a role for these proteins in recombinational repair.

275 r the function of human Rad51C in homologous recombinational repair.

276 of the complex to participate in homologous recombinational repair.

277 ailing to transform yeast cells deficient in recombinational repair.

278 regulation of two proteins that interact in recombinational repair.

279 tenance of chromosomes) complex required for recombinational repair.

280 a key factor in homologous recombination and recombinational repair.

281 ation of ssDNA for checkpoint activation and recombinational repair.

282 anded DNA that is required for RecA-mediated recombinational repair.

283 can be uncoupled from its role in homologous recombinational repair.

284 of histone H2A S129 and concomitant with the recombinational-repair factor Rad52p.

285 the enzymes involved are those that catalyze recombinational-repair processes.

286 lence studies revealed that a highly similar recombinational replacement event underlies an ongoing i

287 pecialized transducing phages (lambdagal) by recombinational rescue of conditionally lethal mutations

288 equence may require efficient RecA-dependent recombinational restart of stalled replication forks.

289 s cerevisiae, using both transcriptional and recombinational "safeguard" control of essential gene fu

290 Both maps confirm the extraordinary recombinational size of the honeybee genome.

291 estructive antiviral state to the productive recombinational state is regulated by the recombination

292 of the Smc5/6 complex in the Mph1-dependent recombinational subpathway that is distinct from Sgs1.

293 e during meiosis, suggesting the presence of recombinational suppressors and/or epistatic gene intera

294 ants, loss of Ies3p delayed the emergence of recombinational survivors and stimulated the formation o

295 The efficiency of recombinational targeting leading to a desired DNA chang

296 It was recently proposed that recombinational telomere elongation (RTE) in a telomeras

297 veromyces lactis mutants lacking telomerase, recombinational telomere elongation (RTE) is induced at

298 Recombinational telomere elongation (RTE) known as alter

299 Different types of recombinational telomere elongation pathways have been i

300 f the INO80 complex, the complex may promote recombinational telomere maintenance by altering chromat