ライフサイエンスコーパス: refold

1 d very high energy barriers of unfolding and refolding).

2 n into inclusion bodies and are difficult to refold.

3 first and then transferred to DnaK2 where it refolded.

4 and ligand binding-dependent conformational refolding.

5 s to undergo ligand-dependent conformational refolding.

6 rin cytoskeleton, including domain unfolding/refolding.

7 onformational changes that trigger F protein refolding.

8 compact and extended states populated during refolding.

9 , and produce 41.5 zJ mechanical work during refolding.

10 e of events could elucidate the mechanism of refolding.

11 ffinity purification and solid-phase protein refolding.

12 s that H223 protonation guards against early refolding.

13 protein aggregates, leading to their native refolding.

14 repeated rounds of stepwise G4-unfolding and refolding.

15 it C-terminus additionally assist in subunit refolding.

16 cient time for protein chaperones to attempt refolding.

17 agglutinin started to undergo conformational refolding.

18 er, enzymatic reactions, and protein un- and refolding.

19 1, to partner with Hsp70 in in vitro protein refolding.

20 transferred to ATP-dependent chaperones for refolding.

21 d using limited proteolysis and denaturation/refolding.

22 and allowed a folding pathway different from refolding.

23 nazole complex, the protein target failed to refold 74 N-terminal residues, suggesting a fundamentall

24 cture, isavuconazole-bound AcCYP51 failed to refold 74 N-terminal residues.

25 photochemical deprotection is sufficient to refold a helical oligomer, interconverting M and P screw

26 one mutation abolished both Hsc70 ATPase and refolding activities.

27 the analyzed mutants show altered ATPase and refolding activity caused by changes in Hsp40 binding.

28 a similar structure, ATP use, and substrate refolding activity, and, importantly, it also inhibits m

29 on path, N(TAIL) typically unfolded and then refolded after additional intermolecular contacts formed

30 nerated aggregated enzymes that could not be refolded after solubilization by denaturation.

31 less than temperature denaturation, protein refolding after a fast P-jump is not necessarily faster

32 Upon refolding after chemical denaturation, this protein prod

33 vinculin and vinculin binding inhibits talin refolding after force is released.

34 e of properly folded rRhi o 2 showed partial refolding after heat denaturation.

35 We proposed a possible "refolding-after-unfolding" mechanism, as further support

36 However, "refolding-after-unfolding" with increasing in-source act

37 plexes with heat shock protein 90 (HSP90) to refold aggregated protein substrates.

38 and the solvent sides of the LSU, where they refold and chemically modify the rRNA and prevent early

39 of CFA/I fimbriae, is able to spontaneously refold and polymerize into cyclic trimers.

40 two structurally different phycobiliproteins refold and reassemble in a cooperative manner from their

41 d ability of disulfide-bonded RRM2 dimers to refold and their increased propensity to misfold, which

42 ss the blood brain barrier help mutant GCase refold and traffic correctly to lysosomes are putative t

43 nins up to 80 degrees C, followed by partial refolding and aggregation at even higher temperatures.

44 mass spectral profiles, which reveal gradual refolding and concomitant deprotonation of higher charge

45 space and integrated with the cell's protein refolding and degradation pathways remains unclear.

46 apable of growing on the ends by seeding the refolding and incorporation of the normal form of the gi

47 s corresponding to HR1 or HR2 interrupt gp41 refolding and inhibit HIV infection.

48 Both experimental refolding and Monte Carlo simulations of Markov state mo

49 ssary early step for paramyxovirus F-protein refolding and presents a novel target for structure-base

50 on in Escherichia coli followed by oxidative refolding and proteolysis.

51 nduced G-quadruplex formation and subsequent refolding and provides key insight into rate-limiting st

52 entropic spring effects can lead to polymer refolding and reformation of the previously cleaved meta

53 is, including quality control during protein refolding and regulation of protein degradation.

54 rol pathways, preferentially Hsp70-dependent refolding and selective autophagy.

55 hibited complex dynamics, including frequent refolding and state occupancies of <10 mus.

56 l-atom simulations of pressure drops capture refolding and unfolding of all three mutants by a simila

57 tigens, this antigen preparation induces MR1 refolding and upregulates surface expression of human MR

58 ctive site was produced in Escherichia coli, refolded, and processed in vitro into a mature enzyme.

59 overexpressed in Escherichia coli BL21(DE3), refolded, and purified.

60 prepared by cell-free expression, functional refolding, and reconstitution into lipid membranes.

61 arance pathways involved in the recognition, refolding, and/or clearance of aberrant proteins.

62 The refolded antigens elicited antibodies that neutralized h

63 ed using typical dialysis- or dilution-based refolding approaches.

64 ecrease in the rates of enzyme unfolding and refolding as well as a reduction of the intrinsic fluctu

65 Refolding assays demonstrate obligate single- and double

66 In vitro refolding assays under redox conditions showed that POFU

67 Here, using in vitro phosphorylation and refolding assays, analytical size-exclusion chromatograp

68 ith fluorescence polarization and luciferase-refolding assays, we report the unexpected discovery of

69 Denaturation and refolding behavior of the allergen confirmed that its Ig

70 -denatured malate dehydrogenase that did not refold by the assistance of the DnaK2 chaperone system a

71 mbrane proteins denatured in SDS can also be refolded by addition of NIS.

72 heir complexes with SDS were attempted to be refolded by the addition of C12E8, while DDM was additio

73 controlled aggregation and facilitates their refolding by ATP-dependent Hsp70-Hsp100 disaggregases.

74 The high thermal stability, refolding capacity, and resistance to gastrointestinal e

75 olution description of unfolded states under refolding conditions for the N-terminal domain of the L9

76 renders automated buffer exchange to screen refolding conditions impossible.

77 by refolding is challenging because suitable refolding conditions must be empirically determined for

78 based determinations with respect to varying refolding conditions.

79 slational modifications, they must either be refolded correctly or recycled.

80 that HSC70 and HSP90 can each independently refold CTA1.

81 a transient encounter complex, allowing the refolded CTD to bind ribosomal protein S10.

82 nd fold rapidly with overlapping melting and refolding curves, G3T multimers (G3T units covalently at

83 Refolded D-Wt molecules form oligomers with high sedimen

84 increases the expression of chaperones that refold damaged misfolded proteins or facilitate their de

85 We present unfolding and refolding data for the small, single-domain protein ddFL

86 hypothesized that much like disaggregation, refolding, degradation, and even normal function, Hsc70

87 oordinated the ability of Hsp22 and Hsp70 to refold denatured luciferase in vitro.

88 The results show that it was not possible to refold denatured proteins with concomitant ligand bindin

89 e resulting method, which we deem DSF guided refolding (DGR), thus enables the production of aggregat

90 Attempts to refold DsbB and GlpG from chemically denatured states in

91 0-gp41 functional interactions affecting Env refolding during HIV entry.

92 olecule, a region that undergoes substantial refolding during host-cell entry.

93 cessible means to characterize the unfolding/refolding dynamics of individual molecules and resolve c

94 Phosphorylation refolds eIF2alpha, allowing it to contact eIF2B at a dif

95 (H or G) triggers F to undergo an extensive refolding event to form a stable postfusion state.

96 Using real-time refolding experiments monitored by CD and NMR, we show t

97 Single-molecule refolding experiments reveal the initial nucleation of f

98 ptical sensing modules with consideration of refolding feature of aptamers, selection of anchoring ph

99 DX is mediated by opening/closing (unfolding/refolding) fluctuations.

100 In this study, we examine the mechanism of refolding for two distinct rhomboids to gain insight int

101 s at low forces enabled the determination of refolding forces of about 2 pN.

102 ces the difference between the unfolding and refolding forces, bringing the non-equilibrium unfolding

103 In vitro refolded forms of tapasin-independent allotypes assemble

104 acteriorhodopsin-I (HmbRI) are obtained, and refolded fractions of HmbRI with this platform are quant

105 hallow trefoil knot, we allow the protein to refold from either the knotted or the unknotted denature

106 by the viral fusogen gB, which is thought to refold from the prefusion to the postfusion form in a se

107 in protein-free RNA but others would need to refold from their dominant solution conformations to for

108 ral membrane proteins have been successfully refolded from solubilized E. coli inclusion bodies.

109 n-prone and disulfide-containing proteins by refolding from E. coli inclusion bodies, which would not

110 As F undergoes a dramatic refolding from its prefusion to postfusion conformation,

111 cid-induced fusion, as well as insights into refolding from pre- to post-fusion conformations.

112 n recruitment, the C-terminal domain of RfaH refolds from an alpha-hairpin, which is bound to RNA pol

113 volves repetitive cycles of G4 unfolding and refolding fueled by ATP hydrolysis.

114 thus far, however, have largely focussed on refolding full-length proteins from artificially induced

115 al reports the crystallographic structure of refolded, full-length SMN protein and two disease-releva

116 Interestingly, DHX36 refolds G4-RNA in several steps, reflecting the discrete

117 nfolding of G4-RNA followed by ATP-dependent refolding, generating a highly asymmetric pattern of act

118 Mammalian prions refold host glycosylphosphatidylinositol-anchored PrP(C)

119 al surface glycoproteins, unleashing them to refold in ways that catalyze virus-cell membrane fusions

120 s expressed in bacteria as inclusion bodies, refolded in vitro and purified for functional and struct

121 Biophysical analysis of constructs refolded in vitro demonstrated that MOSP(C), previously

122 Mini-H2-L(d) refolded in vitro with a high affinity peptide yields a

123 mutants were expressed in Escherichia coli, refolded in vitro, and used to immunize rabbits.

124 g the non-two-state behavior observed during refolding in molecular dynamics simulations.

125 as measured by the inhibition of luciferase refolding in prostate cancer cells.

126 nhibitory effect of TOMM34 on HSP70-mediated refolding in vitro In contrast, we noted that TOMM34 in

127 ve the kinetics of spontaneous unfolding and refolding in zero urea.

128 variant-TCTP11-31 complex reveals that TCTP refolds in a helical conformation upon binding the BH3-g

129 the Mg(2+)-bound state of NCS-1 unfolds and refolds in a three-state process by populating one inter

130 rete steps as force is increased to 7 pN and refolds in reciprocal steps upon force relaxation.

131 Here we report a rapid method for refolding inclusion-body-based, recombinant cell surface

132 At an interface, these cap residues refold, inserting the hydrophobic side chains into the a

133 ampled sequences versus those sequences that refold into the same structure and derive a criterion to

134 rting into the target cell membrane and then refolding into a postfusion structure that fuses the vir

135 rting into the target cell membrane and then refolding into a postfusion structure that fuses the vir

136 ediate structures of the MACPF domain during refolding into the beta-barrel pore establish a structur

137 uses with cells when the gp41 subunit of Env refolds into a 6HB after binding to cellular receptors.

138 ngly compact, and the heme coordination loop refolds into a beta-hairpin with a turn formed by the hi

139 tion wherein the domains dissociate and RfaH refolds into a NusG-like structure.

140 se to facilitate transcription while the CTD refolds into the beta-barrel conformation that interacts

141 activation, the domains separate and the CTD refolds into the beta-barrel, which recruits a ribosome,

142 ss clear how the unbound, sensing riboswitch refolds into the ligand binding-induced output state.

143 s to a lower volume monomeric PrP state that refolds into the native conformation after pressure rele

144 Domain 3 of the soluble toxin refolds into two 85 A beta-hairpins that traverse the li

145 ReFOLD is a novel hybrid refinement server with integrat

146 the non-native client proteins bound to HtpG refold is of central importance to comprehend the essent

147 In practice, however, protein production by refolding is challenging because suitable refolding cond

148 Loop refolding is limited by the hydrophobic collapse of the

149 ied the antibody from Escherichia coli after refolding it from inclusion bodies.

150 NTE as influencing the chaperone-independent refolding kinetics and overall thermodynamic stability o

151 we report the force-dependent unfolding and refolding kinetics of all talin rod domains.

152 of microsecond pressure and temperature jump refolding kinetics of the engineered WW domain FiP35, a

153 We compared the NMR-derived refolding kinetics with data derived from thermal hyster

154 of folding against force and accelerates the refolding kinetics.

155 n-random client proteins of the Hsp104/Hsp70-refolding machinery, including the prion Sup35.

156 omes bind to a mRNA faster than the mRNA can refold, maintaining it in a nonequilibrium state with an

157 es involved in normal protein maturation and refolding malformed proteins through the unfolded protei

158 in-coupled receptor CXCR1 was obtained using refolded material from E. coli inclusion bodies.

159 Because MSD refolding may turn off GPIb-IX's mechanosensory signals,

160 ocyanin secondary structure but alters their refolding mechanism and dodecameric structure.

161 ts demonstrate a cooperative, self-chaperone refolding mechanism, whereby the beta-subunits independe

162 Thus, activation or restoration of refolding mechanisms may alleviate TDP-43 aggregation in

163 To investigate the dynamic nature of MHC, we refolded MHC proteins with peptides that can be hydrolyz

164 BiP's ATPase activity, which is required for refolding misfolded proteins while coping with ER stress

165 These experiments reveal that refolding monitored by 1-3 contact formation indeed is m

166 ation is inhibited and with that of in vitro refolded mRNA.

167 s tested, arginine was the most effective in refolding mutant of pVHL.

168 We observe that the refolding nature of the aptamer and its combination with

169 rs in one discrete step at forces >10 pN and refolding occurred at lower forces showing hysteresis.

170 our results suggest that initial Luciferase refolding occurs along a vectorial pathway and also sugg

171 we measure the amount of ATP used for native refolding of a misfolded group I intron ribozyme by CYT-

172 s of the temperature dependent unfolding and refolding of a titin immunoglobulin domain and alpha-act

173 promotes HeLa cell survival and enhances the refolding of an Hsp90 substrate inside the cell.

174 The structures also reveal refolding of an S1 subdomain after ACE2 binding that dis

175 f the SDS from the protein-SDS complexes and refolding of betaLG, BSA, and lysozyme, while alphaLA ch

176 Refolding of both proteins results in reassociation of t

177 er-domain packing through a short linker and refolding of CD2.

178 f HMLalpha as the donor, when we perturb the refolding of chromosome III.

179 helix lead to increased stability and faster refolding of collagen peptides containing aza-glycine.

180 haperones Ydj1 and Sis1, and do not catalyze refolding of denatured proteins in vitro in cooperation

181 Refolding of disordered CTA1 occurred in the presence of

182 hundred ATP molecules are hydrolyzed during refolding of each ribozyme molecule.

183 tained from stopped-flow measurements of the refolding of Escherichia coli adenylate kinase were anal

184 other DNA polymerases, or hRev1 can prevent refolding of G4 DNA structures.

185 the activation energy required to cause the refolding of gB from a prefusion to a postfusion conform

186 ical energy through mechanical unfolding and refolding of isopeptide bond-delimited polypeptide loops

187 to chaperones, which aid in the clearance or refolding of misfolded proteins.

188 e native folding of nascent polypeptides and refolding of misfolded species, thereby buffering mutati

189 At slow pulling speeds (<50 nm s(-1)), the refolding of NuG2 can be clearly observed.

190 In addition, helix-structural refolding of PDGF binding aptamers (PBA) indirectly wrap

191 sms controlling the mechanical unfolding and refolding of proteins cannot be accessed by protein fold

192 talytic enzyme reaction is used to stimulate refolding of proteins during real-time analysis.

193 l as prolamin precursor proteins involved in refolding of proteins.

194 rences between protein folding in the ER and refolding of purified proteins.

195 pe III secretion, although contribution from refolding of secreted proteins has not been ruled out.

196 ozymes could be restored by putative t2M/t4M refolding of stem secondary structure or tertiary bridgi

197 hat force-dependent stochastic unfolding and refolding of talin rod domains make talin a very effecti

198 Surprisingly, we find that during refolding of tandem repeats, independent of sequence ide

199 Defective refolding of TDP-43 is predicted to aggravate the TDP-43

200 In vitro, OsmY assisted in the refolding of the antigen 43 beta-barrel domain and prote

201 -n-butylammonium chloride results in dynamic refolding of the catalyst from the native fold to the an

202 ring, which enables reversible unfolding and refolding of the chains.

203 chemokine receptors on target cells triggers refolding of the gp41 transmembrane subunit into a six-h

204 diated GQ unfolding is typically followed by refolding of the GQ, a pattern that is repeated several

205 dependent conformational destabilization and refolding of the hole-hole homodimer Fc.

206 terized the molecular events associated with refolding of the metastable prefusion S glycoprotein to

207 er virus, we show that these changes involve refolding of the protein into a trimeric state.

208 and peptides suggests that SER5 also delays refolding of the remaining fusion-competent Env trimers.

209 observed in the p53-MDM2 complex and induces refolding of the short, unstructured MDM2 N-terminal reg

210 ified based on changes in the elasticity and refolding of the unfolded polyprotein in the presence of

211 Here, we study pressure-drop refolding of three lambda-repressor fragment (lambda(6-8

212 pendent activation, titin elastic recoil and refolding of titin domains as an energy source, and Ca(2

213 output: conformational changes that trigger refolding of trimeric fusion proteins and membrane fusio

214 e MACPF domain, accompanied by extrusion and refolding of two alpha-helical regions into transmembran

215 DnaK2 chaperone system, HtpG enhanced native refolding of urea-denatured lactate dehydrogenase and he

216 These results establish refolding of yeast chromosome III as a key driving force

217 manently folded but spontaneously unfold and refold on time scales that can span orders of magnitude

218 ressure-drop relaxation experiments captured refolding on a millisecond time scale.

219 finity interaction leading to conformational refolding on a ~1-s timescale at 36 degrees C.

220 endent manner, and supports their productive refolding once nonstress conditions are restored.

221 to dissociate aggregates and thereby enables refolding or degradation of misfolded proteins.

222 by unfolding aberrant and toxic proteins for refolding or proteolytic degradation.

223 e, or does the extraction process facilitate refolding (or unfolding)?

224 f leaving after G4 unwinding, allowing it to refold, or going beyond to unwind duplex DNA, Pif1 repea

225 key conformational changes in the F-protein refolding pathway, but a detailed understanding of prefu

226 nd active (beta-barrel) states and plausible refolding pathways have been reported, how this reversib

227 this modelling indicates whether alternative refolding pathways might occur upon cooling.

228 However, aggregation during the refolding process is a common difficulty, which is often

229 rotein, which undergoes a major irreversible refolding process to merge the two membranes.

230 n (F) protein, the latter undergoing a major refolding process to merge the two membranes.

231 residues function as hinge positions in the refolding process, which closes the secondary ligand sph

232 The associated refolding processes often cannot be explained by thermod

233 for dynamic, interconverting, unfolding, and refolding proteins.

234 dest yield but required the incorporation of refolding protocols to obtain a proper conformation.

235 cooperates with the DnaK chaperone system to refold purified luciferase.

236 allow the completely unfolded Luciferase to refold quickly in AFM experiments, strongly suggesting t

237 The unstressed refolding rate of MSD is ~17 s(-1) and slows exponential

238 d-type proteins indicates the differences in refolding rates may be correlated with the degree of fru

239 onential force dependencies of unfolding and refolding rates.

240 through all-or-none transitions with similar refolding rates.

241 mutation in reconstituted disaggregation or refolding reactions in vitro.

242 This article discusses the structures and refolding reactions of specific fusion proteins and the

243 itiate thermodynamically favorable unfolding-refolding reactions that release the (DMA)C-labeled stra

244 rces, bringing the non-equilibrium unfolding-refolding reactions towards equilibrium.

245 Native BmAMA1 from parasite lysate and refolded recombinant BmAMA1 (rBmAMA1) expressed in Esche

246 at belong to the same supertype, and, during refolding, reduced aggregation of tapasin-independent al

247 Notably, a pH-dependent refolding region (residues 824-858) at the spike-interdo

248 e prevention of hinge movements in the first refolding region and the elimination of proteolytic expo

249 he N-terminal helix of S-peptide unfolds and refolds repeatedly on the microsecond timescale, indicat

250 absence of divalent ions, NCS-1 unfolds and refolds reversibly in a two-state reaction involving onl

251 at approximately 100 ATPs are hydrolyzed per refolded RNA as a consequence of interactions specific t

252 with SDS, making it possible to release and refold SDS-denatured proteins by adding sufficient amoun

253 HSP90 also refolds some endogenous cytosolic proteins as part of a

254 -type and adapted HIV-1 isolates, early gp41 refolding steps obligatorily occur on cell surfaces, whe

255 antibodies in engineered cells often require refolding steps or secretion across one or more biologic

256 e demonstrate that, compared to the in vitro refolded structure, in vivo IAV mRNAs are less structure

257 duced unfolding experiments and stopped-flow refolding studies at ambient pressure, NMR-detected pres

258 showed an ATP-ase activity and an ability to refold substrates identical to the wild type.

259 nsive mechanism to promote RNA unfolding and refolding, suggesting an evolutionary convergence with p

260 und motors toward the center of the cell, or refold the cargo-free motors into an inactive state read

261 their ability to suppress aggregation of and refold the model substrate gammad-crystallin, suppress a

262 ppress aggregation of mutant huntingtin, and refold the physiological substrate beta-actin in vitro.

263 ptide into the target cell membrane and then refold the trimeric assembly into an energetically stabl

264 /mol that represents the free energy cost of refolding the oligomeric intermediate into the structure

265 and facilitates its ER-to-cytosol export by refolding the toxin as it emerges at the cytosolic face

266 Nef refolds the first alpha-helix of the beta2 subunit of AP

267 ism, whereby the beta-subunits independently refold, thereby templating the folding of the alpha-subu

268 ge of folding newly synthesized proteins and refolding those that have become misfolded in the contex

269 branes together as two heptad-repeat regions refold to form a six-helix bundle structure that can be

270 Remarkably, both SpaA and FimA initially refold to mechanically weaker intermediates that recover

271 tein, to insert into the target membrane and refold to merge the viral and cellular membranes.

272 ntrinsic instability, cytosolic CTA1 must be refolded to achieve an active conformation.

273 ribosomal state to allow for mRNA structure refolding to drive large-scale ribosome movements.

274 nts that facilitate their flexible C-termini refolding to engage distinct interfaces.

275 ormational changes that energetically couple refolding to membrane fusion.

276 eins from the aggregates and assist in their refolding to the native state.

277 s F structures demonstrates that a conserved refolding trajectory mediates entry of these viruses and

278 tandem hairpins displayed ensemble unfolding/refolding transitions, which were exploited to recognize

279 The solid support allows for multiple refolding trials through buffer exchanges, and the EPR s

280 uction and oxidation strategy to efficiently refold two high-yielding monomeric E2 species, D123 and

281 dependent effect by increasing the amount of refolded type III collagen in vitro and FKBP19 seems to

282 The failure of AcCYP51 to fully refold upon inhibitor binding in vivo would cause an irr

283 her globular proteins unfolded by SDS can be refolded upon addition of C12E8 and DDM.

284 events their aggregation, and supports their refolding upon subsequent neutralization.

285 uses the target domain to first unfold, then refold via domain swap.

286 centrations to display GQ-like unfolding and refolded via intermediates, contrary to the wild type.

287 ReFOLD was used to generate hundreds of alternative refi

288 Refolding was confirmed by ultrafast broadband transient

289 and hysteresis in the thermal unfolding and refolding was observed for all proteins.

290 Refolding was rapid and stochastically redistributed mol

291 ding states of cyt c in the early 500 mus of refolding was revealed on the microsecond time scale.

292 lysis that occurs in the absence of ribozyme refolding, we find that approximately 100 ATPs are hydro

293 ing the rates of ATP hydrolysis and ribozyme refolding, we find that several hundred ATP molecules ar

294 lling speeds (ca. 2 nm s(-1)), unfolding and refolding were observed to occur in near equilibrium.

295 on the assumption of DSB-induced chromosome refolding, which also takes into account the previously

296 the DSB is induced chromosome III undergoes refolding, which directs the MAT locus to recombine with

297 jects stable RNAs, whereas unstable RNAs are refolded while bound to the enzyme and subjected to a se

298 Importantly, the unfolded MSD can refold with or without applied forces.

299 The SA-binding core domain of NPR4 refolded with SA adopts an alpha-helical fold that compl

300 n this complex, the alpha subunits of Hb are refolded with the heme displaced to the interface with I

301 EPR spectra at each state of the refolding workflow of spin-labeled Haloarcula marismortu