ライフサイエンスコーパス: rejoin

1 gments of the severed axon were permitted to rejoin.

2 role when previously allopatric populations rejoin.

3 5'-phosphate termini that can be efficiently rejoined.

4 ds to radiation sensitivity and impaired DSB rejoining.

5 d after chromosome breakage and illegitimate rejoining.

6 quences of axotomy were suppressed by axonal rejoining.

7 repair (HRR) is at a stage after the initial rejoining.

8 M059-K cells and strongly inhibited DNA DSB rejoining.

9 mportance of protein kinase activity for DSB rejoining.

10 hich bridge the broken chromosome and direct rejoining.

11 ies on two catalytic steps, DNA breakage and rejoining.

12 so inhibits DNA ligase IV/XRCC4-mediated end rejoining.

13 lic attack by the 3'-OH end to result in DNA rejoining.

14 cement of DNA cleavage and inhibition of DNA rejoining.

15 on, F96-224 cells show slow but residual DSB rejoining.

16 as well as alternative mechanisms for break rejoining.

17 es from RAD52/RAD52 strains exhibited slower rejoining (10 min to 4 hr in growth medium).

18 Participants were required to rejoin a previously learned route encountered from an un

19 racts of CHO-K1 cells are able to accurately rejoin a site-specific free radical-mediated double-stra

20 us recombination (HR) contributes to DNA DSB rejoining, a systematic genetic study was undertaken usi

21 A chromatin-based model of DNA DSB rejoining accommodating biochemical and genetic results

22 cribe a bacterial enzyme with processing and rejoining activities encoded in a single polypeptide cha

23 emoval activity but not the DNA cleavage and rejoining activities.

24 umorigenic and had a normal DNA strand break rejoining activity and normal radiosensitivity in respon

25 air, but also enhanced double-strand DNA end-rejoining activity in hippocampal neurons.

26 ds that require ligase because the rapid DNA rejoining activity of Vaccinia topoisomerase I allows li

27 sensory neurons, we investigated how axonal rejoining alters the cellular consequences of axotomy.

28 at this protein damage compromises DNA break rejoining and base and nucleotide excision repair.

29 lls exhibit higher double-strand break (DSB) rejoining and display lower levels of residual DSBs.

30 e same enzymes also participate in imprecise rejoining and joining of incompatible ends, important mu

31 Inhibitors of the second step prevent DNA rejoining and lead to persistent DNA breaks, acting as t

32 ortmannin had only a small effect on DNA DSB rejoining and no effect on cell radiosensitivity to kill

33 itor B cells, deletion of Ku70 increases DSB rejoining and translocation levels to those observed in

34 DNA-PKcs kinase activity is required for DSB rejoining and V(D)J recombination and show that the extr

35 represents an ancestral population split and rejoin, and demonstrate its application on simulated and

36 RAG-1 or RAG-2 possess comparable cleavage, rejoining, and end-processing activity, as well as simil

37 its N-terminal region, promotes precise DSB rejoining, and increases cellular resistance to radiatio

38 iosensitivity, decreased double-strand break rejoining, and reduced fidelity in signal and coding joi

39 iate this impressive acceleration of DNA DSB rejoining, and regions of chromatin within a certain ran

40 data suggest RAG-mediated transposition and rejoining are differentially regulated by the full-lengt

41 ted pathway of DNA-PK-dependent direct break rejoining are only of minor importance.

42 directed double-strand (ds) DNA breakage and rejoining are part of the physiologic program of lymphoc

43 angements arose by chromosome shattering and rejoining as well as by replication-dependent mechanisms

44 Furthermore, using an in vivo plasmid rejoining assay, we demonstrate that SIR2, SIR3 and SIR4

45 Indeed, through using an in vivo plasmid rejoining assay, we find that YKU80 plays an essential r

46 ing a rapid and reproducible in vivo plasmid rejoining assay, we show that Yku70p plays a crucial rol

47 g a combination of genetics and cellular DNA rejoining assays, in this issue of Molecular Cell, Wyatt

48 s established that mitochondria fragment and rejoin at distinct stages during meiosis and sporulation

49 contralateral side, early GON branching with rejoining at a distal point, and a connection between th

50 The resolution reaction entails cleavage and rejoining at CCCTT/N recognition sites arrayed on opposi

51 Extracts depleted of Ku and DNA-PKcs rejoin blunt ends, as well as homologous ends with 3' or

52 The ability to rejoin broken chromosomes is fundamental to the maintena

53 evolved to repair these cytotoxic lesions by rejoining broken ends, among them the nonhomologous end-

54 an cells, non-homologous end joining (NHEJ), rejoins broken DNA ends by direct ligation.

55 while a vector with mismatched ends was also rejoined but yielded a mixture of products.

56 half-times of either of these components of rejoining but increased from 17 to 72% the proportion of

57 nin and dimethylaminopurine not only blocked rejoining, but also suppressed phosphoglycolate removal,

58 (H) exons, which are processed into DSBs and rejoined by C-NHEJ to complete CSR.

59 fills the gap, and the DNA ends are finally rejoined by DNA ligase.

60 Broken DNA ends are rejoined by non-homologous end-joining (NHEJ) pathways r

61 The AP-site is rejoined by short-patch base excision repair when the le

62 DSBs) induced by topoisomerase II (TOP2) are rejoined by TDP2-dependent non-homologous end-joining (N

63 These vectors were rejoined by the extracts at rates 30-100 times slower th

64 Such DSBs are normally rejoined by TOP2 but on occasion can become abortive and

65 xoguanine, reducing gap filling and accurate rejoining by at least 10-fold.

66 the enzyme-DNA interactions required for DNA rejoining by bacterial type IA topoisomerases could be d

67 oken DNA ends physically, facilitating their rejoining by DNA ligases.

68 DNA breakage and rejoining by either a type IA or a type IIA enzyme are s

69 icipate in the catalysis of DNA cleavage and rejoining by the site-specific recombinase Tn3 resolvase

70 ide polymerization by primases and in strand rejoining by topoisomerases and as a general acid in str

71 al results demonstrate that catalysis of DNA rejoining by type IA topoisomerases has a more stringent

72 e II cleave the initial DNA substrate before rejoining can be monitored.

73 e conclude that the Zn-f may enable Lig-3 to rejoin chromosomal DNA strand breaks located at sites of

74 NLT can rapidly break and rejoin chromosomes and thus could have played an importa

75 es advantage of the fact that the enzyme can rejoin cleaved nucleic acids but cannot mediate DNA scis

76 chanical and electrical properties even when rejoining cut surfaces after long exposure times.

77 ortance of the topoisomerase II DNA cleavage/rejoining cycle to genomic integrity, the mechanistic de

78 gregating acentric chromosome fragments that rejoin daughter nuclei are associated with nuclear membr

79 sms allowing late-segregating chromosomes to rejoin daughter nuclei remain underexplored.

80 cell lines share the DNA double strand break rejoining defect of M059-J and irs20 cells, the lack of

81 -1 (PARP-1) participates in DNA cleavage and rejoining-dependent reactions, such as DNA replication,

82 suggest that complex DSBs can be accurately rejoined despite the presence of accompanying base damag

83 DNA double strand breaks (DSBs) can be rejoined directly by the nonhomologous end-joining (NHEJ

84 two cell lines exhibited impaired ability to rejoin DNA double-strand breaks on pulsed-field gel elec

85 Type IB topoisomerases cleave and rejoin DNA strands through a stable covalent DNA-(3'-pho

86 Type IB topoisomerases cleave and rejoin DNA through a DNA-(3'-phosphotyrosyl)-enzyme inte

87 Ialpha (hTOPO IIIalpha), which can break and rejoin DNA to alter its topology.

88 h an inactivating mutation in DNA ligase IV, rejoined DNA DSBs predominantly with slow kinetics simil

89 an activity consistent with a direct role in rejoining DNA breaks and independent of DNA-dependent pr

90 logous end-joining (NHEJ) repair pathway for rejoining DNA double strand breaks.

91 Cutting and rejoining DNA occurs within an intermediate synaptic com

92 e topological tension in DNA by breaking and rejoining DNA phosphodiester bonds.

93 elaxation of supercoiled DNA by cleaving and rejoining DNA strands through a DNA- (3'-phosphotyrosyl)

94 elaxation of supercoiled DNA by cleaving and rejoining DNA strands via a pathway involving a covalent

95 merases control DNA topology by breaking and rejoining DNA strands via covalent complexes with cleave

96 are known to serve the role of breaking and rejoining DNA strands, the present findings suggest that

97 nuclease in vivo by recognizing and cleaving/rejoining DNA structures with single-stranded character.

98 Vaccinia DNA topoisomerase breaks and rejoins DNA strands through a DNA-(3'-phosphotyrosyl)-en

99 tially attempts to repair DSBs and, if rapid rejoining does not ensue, then resection occurs promotin

100 repair system are also involved in NHEJ, the rejoining does not involve all of the homologous recombi

101 est that DNA polymerase alpha may be able to rejoin double-strand breaks in vivo during replication.

102 rs and use RNase H-like domains to break and rejoin double-stranded DNA (dsDNA), Helitron transposase

103 After irradiation, the 28SC cells rejoined double-strand breaks efficiently within 24 h.

104 ines suggests that mammalian cells primarily rejoin DSBs by nonhomologous mechanisms, alternative mec

105 ination, showing that NHEJ also functions to rejoin DSBs introduced during lymphocyte development [7]

106 us end joining (NHEJ), which is required for rejoining DSBs during VDJ recombination.

107 edominant in the G1 phase of the cell cycle, rejoins DSBs either accurately or with errors, but the m

108 Here, we show that TDP2 rejoins DSBs induced during transcription-dependent TOP2

109 aling energy is a primary determinant of the rejoining efficiency and mechanism.

110 irectly involved in this regulation, because rejoining enhancement was dependent on the presence of n

111 ngth chromosomes by a dedicated DNA cleavage-rejoining enzyme known as a hairpin telomere resolvase (

112 r chromosomes are generated by a DNA cutting-rejoining enzyme protelomerase.

113 al analysis of other 'tyrosine' DNA-breaking/rejoining enzymes with similar enzyme mechanisms, namely

114 odel for the order and types of cleavage and rejoining events in the t(14;18) translocation.

115 s of carefully orchestrated DNA breakage and rejoining events.

116 DNAs from both genotypes exhibited quick rejoining following gamma irradiation and sedimentation

117 he time has come for geriatric psychiatry to rejoin geriatric medicine so that psychiatry can recaptu

118 RAD51B, RAD52 and RAD54 leaves unchanged the rejoining half times and the contribution of the slow co

119 of how DNA ds breaks are both generated and rejoined has increased.

120 CSR involves breaking and rejoining highly repetitive switch (S) regions in the Ig

121 ror-free repair) or sequence alteration upon rejoining (i.e., error-prone or mutagenic repair).

122 We suggest that a rejoined Ig gene may not merely be a sequence restrictin

123 TOP2-induced DNA double-strand breaks are rejoined in part by tyrosyl-DNA phosphodiesterase 2 (TDP

124 liquid bolus was split around the larynx and rejoined in the hypopharynx.

125 hifts from the fast to the slow component of rejoining in BRCA2-proficient and BRCA2-deficient cells.

126 as somewhat unexpected, because signal joint rejoining in cells from patients with Nijmegen breakage

127 that result from double-strand breakage and rejoining in cells of the skin in which p53 is inactivat

128 produced only a modest inhibition in DNA DSB rejoining in M059-J cells, suggesting that, for these en

129 efficiency of DNA double strand break (DSB) rejoining in primary cells from mouse strains that show

130 lymerase can mediate DNA double strand break rejoining in the absence of other proteins.

131 is consistent with the hypothesis that V(D)J rejoining in the majority, at least, of A-T patients may

132 , is sufficient to induce V(D)J cleavage and rejoining in this lower eukaryote.

133 ect as TERT does not directly affect DNA end rejoining in vitro or meiotic recombination in vivo.

134 t this was restored when the TCR chains were rejoined into disulfide-linked alphabeta heterodimers.

135 tal segment was left unmolested, then axonal rejoining invariably occurred within 7 hr.

136 l that activation of DNA strand cleavage and rejoining involves large conformational changes and DNA

137 e (BxPC3) or mutant forms (Capan-1) of BRCA2 rejoin IR-induced DNA DSBs to a similar extent following

138 We show that DT40 cells rejoin IR-induced DNA DSBs with half times of 13 min and

139 Moreover, we show that the rejoining is fully dependent on DNA ligase IV, indicatin

140 ion of the role of these proteins in DNA DSB rejoining is important for their functional characteriza

141 However, DNA rejoining is inhibited in the absence of this conserved

142 or the slow, DNA-PK-independent component of rejoining is not affected by mutations in BRCA2.

143 d DSBs was confirmed via the analysis of DSB rejoining kinetics using pulsed field gel electrophoresi

144 from complementation groups A, C, D2, and G rejoined linearized plasmids with a significantly decrea

145 rom PC12-Tat cells compared to PC12 cells in rejoining linearized DNA.

146 re, the yeast NHEJ proteins, and alternative rejoining mechanisms influence the accuracy of break rep

147 XRCC4/ligase IV), were capable of accurately rejoining model double-strand break substrates with a 1-

148 Molecular analysis of rejoined molecules revealed that >95% of the linearized

149 ring suggested that chromosomal 5' DSBs were rejoined more efficiently than 3' DSBs, consistent with

150 neurons were found to enter, leave and then rejoin neural networks, and may constitute the memory tr

151 entional double-strand (ds) DNA breakage and rejoining occur during lymphoid differentiation.

152 rase beta and DNA ligase III-XRCC1, accurate rejoining of a 3' mismatched base residue at a single-st

153 e a DNA-editing function in vitro, promoting rejoining of a 3' mismatched residue in a reconstituted

154 ter system that rapidly assesses the correct rejoining of a restriction-enzyme produced DSBs within t

155 used to demonstrate sequential breaking and rejoining of a specific nucleic acid.

156 N-terminal toroid structure) for cutting and rejoining of a ssDNA strand can be combined with two dif

157 Rejoining of broken chromosome ends (end-joining) near t

158 and break (DSB) repair pathways catalyze the rejoining of broken chromosomes and the integration of t

159 Rejoining of broken DNA molecules in the absence of Ku r

160 iquitous DNA repair factors then mediate the rejoining of broken DNA.

161 mbination, the precise physical breakage and rejoining of DNA between homologous chromosomes, plays a

162 more directly involved in the processing and rejoining of DNA breaks.

163 nd-joining pathway promotes direct enzymatic rejoining of DNA double-strand breaks (DSBs) and is an i

164 inase that is required for proper end-to-end rejoining of DNA double-strand breaks [3].

165 een the nonhomologous end-joining system for rejoining of DNA double-strand breaks and the ATM-depend

166 Nonhomologous end joining (NHEJ), the direct rejoining of DNA double-strand breaks, is closely associ

167 NA-PKcs, in addition to its functions in the rejoining of DNA dsb and in DNA replication, also operat

168 We compared rejoining of DNA DSBs in a human glioma cell line, M059-

169 se activity of DNA-PKcs is essential for the rejoining of DNA DSBs in mammalian cells.

170 In both cell lines, rejoining of DNA DSBs was biphasic, with a fast and a sl

171 of magnitude leave unchanged the kinetics of rejoining of DNA DSBs, and fail to modify the contributi

172 The covalent rejoining of DNA ends at single-stranded or double-stran

173 ification is controlled in vivo by efficient rejoining of DNA ends generated during V(D)J recombinati

174 le for mammalian DNA polymerase theta in the rejoining of DNA ends that are poor substrates for class

175 with its cofactor TDRD3 during cleavage and rejoining of DNA or RNA, thus elucidating the roles of d

176 hough this domain catalyzes the cleavage and rejoining of DNA strands it, unexpectedly, does not form

177 IB topoisomerases catalyze the cleavage and rejoining of DNA strands through a DNA-(3'-phosphotyrosy

178 id type I enzyme, catalyzes the cleavage and rejoining of DNA strands through a DNA-(3'-phosphotyrosy

179 ve site tyrosine Tyr-782 in the breakage and rejoining of DNA strands.

180 omerases act similarly in their cleavage and rejoining of DNA.

181 involved in Mg(II) binding and the cleavage-rejoining of DNA.

182 double-stranded DNA and is required for the rejoining of double-stranded DNA breaks in mammalian cel

183 tion-induced DSBs and contributes to the mis-rejoining of drug-induced DSBs in BRCA1-deficient cells

184 dence to show that BRCA1 promotes error-free rejoining of DSBs in human breast carcinoma cells while

185 Loss of Pol4 did not affect the rejoining of DSBs that utilized a recessed microhomology

186 ound that presence of wild-type p53 enhanced rejoining of DSBs with short complementary ends of singl

187 which prevents A-NHEJ-dependent short-range rejoining of intra-switch region DSBs.

188 Rejoining of ionizing radiation (IR) induced DNA DSBs us

189 The role of BRCA1 and BRCA2 in the rejoining of ionizing radiation (IR)-induced DNA DSBs, w

190 Detection and rejoining of ionizing radiation-induced DNA dsb proceede

191 er cell line with mutated BRCA1 shows normal rejoining of IR-induced DNA DSBs and levels of inhibitio

192 utilization of NHEJ as the main pathway for rejoining of IR-induced DNA DSBs and speculate that the

193 firm a direct role for BRCA1 or BRCA2 in the rejoining of IR-induced DSBs in the genome of human tumo

194 ted sequences (IES) are excised, followed by rejoining of MAC-destined sequences, while fragmentation

195 on repair system reconstituted in vitro, the rejoining of nicked mismatched DNA depended on the prese

196 of the DNA at segment boundaries followed by rejoining of particular pairs of the resulting termini.

197 In addition, rejoining of radiation-induced DNA DSBs, that mainly ref

198 to this DNA breakage and cells defective in rejoining of S-phase DSB are hypersensitive to the combi

199 alternative DNA rearrangements in vivo: the rejoining of signal and coding ends and the transpositio

200 Rejoining of single- and double-strand breaks (DSBs) int

201 t repair pathways is the DNA ligase-mediated rejoining of single- and double-strand breaks.

202 rough perturbation of any steps prior to the rejoining of the 60S ribosomal subunit during the entire

203 reas when the lesions are 3' to one another, rejoining of the AP-site occurs by both long-patch and s

204 of DNA through the resulting break, and the rejoining of the broken phosphodiester backbone.

205 resulting in concerted cleavage and directed rejoining of the DNA ends.

206 CR) events result from abnormal breaking and rejoining of the DNA molecules, or from crossing-over be

207 sion, and centromere misdivision followed by rejoining of the resulting fragments.

208 somerases alter DNA topology by cleaving and rejoining one strand of duplex DNA through a covalent pr

209 oduced into S mu during CSR, with some being rejoined or joined to each other to generate internal sw

210 EJ), the major DNA double strand break (DSB) rejoining pathway.

211 Gs based on the site of polymerase break and rejoin, PCR-based tools cannot provide full-length seque

212 endent on DNA ligase IV, indicating that the rejoining phase relies on the nonhomologous DNA end-join

213 in bovine aorta, and a gel sleeve is able to rejoin pieces of a severed gel tube.

214 re are no genome sequence-specific breaks or rejoin points for the generation of copyback DVGs, genom

215 appears to be no sequence-specific break or rejoining points for the generation of copyback DVGs, ou

216 lu-9 has a critical role in DNA breakage and rejoining, probably through its interaction with the 3'

217 R2, SIR3, SIR4 or SRS2, suggesting that this rejoining process occurs by a different mechanism.

218 bination is a double-strand DNA breakage and rejoining process that relies on NHEJ for the joining st

219 o their distinct apoptotic response, neurons rejoined radiation-induced DNA double-strand breaks more

220 ls show similar induction levels and similar rejoining rates of DNA double strand breaks (DSBs) follo

221 ATRkd) cells have the similar inductions and rejoining rates of DNA DSBs compared with cells without

222 would be expected if sister chromosomes are rejoined, rather than the 3:1 ratio typical of a Mendeli

223 ereochemical outcome of the net cleavage and rejoining reaction established that the reaction proceed

224 relaxes superhelical DNA through a breakage/rejoining reaction in which the active site tyrosine lin

225 rmediate through a two-step DNA cleavage and rejoining reaction referred to as telomere resolution.

226 xact function of this protein complex in the rejoining reaction remains to be elucidated.

227 s inhibit the rejoining step of the breakage/rejoining reaction, which traps the enzyme in covalent l

228 XerC and XerD, catalyse strand cleavage and rejoining reactions at a site, dif, in order to ensure n

229 of these elements catalyzes DNA breakage and rejoining reactions required for transposition.

230 of these elements catalyzes DNA breakage and rejoining reactions required for transposition.

231 Function of the rejoined sciatic nerve was measured by contraction of th

232 0(-/-) cells lack the ability to effectively rejoin signal and coding ends liberated in transiently i

233 Tyrosine recombinases break and rejoin single strands in pairs to form a Holliday juncti

234 by general acid-base catalysis to break and rejoin single-stranded DNA.

235 enanes, knots and supercoils by breaking and rejoining single-stranded DNA/RNA segments and allowing

236 of fusion, but nevertheless differed in the rejoined state of the system.

237 mptothecin (CPT) and its analogs inhibit the rejoining step of the breakage/rejoining reaction, which

238 rugs and lesions that interfere with the DNA rejoining step.

239 d 3-9-fold less DNA end joining activity and rejoined substrates with significantly less fidelity tha

240 cles either untreated or cut and immediately rejoined surgically many months earlier.

241 luded from the aggregates, they subsequently rejoin the aggregate and produce spores.

242 t migrated between EC junctions were able to rejoin the blood circulation.

243 Thus, T(RM) cells can rejoin the circulation but are advantaged to re-form loc

244 one of the major DSB repair pathways and can rejoin the DSB ends either precisely or with mistakes.

245 uster near the midbody gradually migrates to rejoin the major cluster on the far side of the nucleus

246 with a half time of 18.4 h, is still able to rejoin the majority (63%) of DSBs.

247 IL-10+ Tr1 cells arise from memory cells and rejoin the tissue-resident memory T-cell pool after cess

248 M) cells, former intestinal T(RM) cells that rejoined the circulating pool, heritably maintained a pr

249 We observed that reactivated T(RM) cells rejoined the circulating pool.

250 ratory of Molecular Biology in Cambridge, he rejoined the ICRF as a Research Scientist at the Develop

251 gate (opening), and then closing the gate by rejoining the broken ends.

252 t postmitotic repair of a broken chromosome (rejoining the centric and acentric fragments) occurred i

253 nd-joining (NHEJ) pathway is responsible for rejoining the majority of double-strand breaks in mammal

254 ' end to reduce the possibility of repair by rejoining the nicked ends.

255 splaced from a trail know which way to go on rejoining the trail?

256 EJ is DNA ligase IV/XRCC4/XLF complex, which rejoins the DNA.

257 covalently linked to the linear strand, that rejoins the ends by a reversible transesterification rea

258 Mitochondrial RNA ligase subsequently rejoins the mRNA.

259 , passes another through the break, and then rejoins the severed ends.

260 tant conditions, the two activity components rejoin through a series of transients lasting 2-7 days.

261 , the cleaved coding and signal ends must be rejoined to generate functional antigen receptors and ma

262 st pathological double-strand DNA breaks are rejoined to restore chromosomal integrity are the same.

263 tein forms a complex with DNA ligase IV that rejoins two DNA ends in the last step of V(D)J recombina

264 It is proposed that they use a breaking-and-rejoin type mechanism to affect DNA rearrangement on spe

265 tigs (scaffolds) were iteratively broken and rejoined using several criteria, yielding a 64-fold incr

266 ation, transposition, and low-efficiency TIR rejoining using reaction mechanisms similar to those use

267 gated with different transgenic vectors, and rejoin via protein trans-splicing to reconstitute a full

268 nia topoisomerase catalyzes DNA cleavage and rejoining via transesterification to pentapyrimidine rec

269 DNA DSB rejoining was nearly complete in both cell lines, and th

270 Three mechanisms of rejoining were observed, regardless of overhang polarity

271 reconstitutes efficient double-strand break rejoining when it is added to a reaction containing the

272 e vicinity of the DNA DSB determines whether rejoining will follow fast or slow kinetics.

273 lei retain DNA damage markers and frequently rejoin with the parent nucleus.

274 o and carboxyl portions are interchanged and rejoined with a short spacer connecting the original ter

275 ofoundly decreases the proportion of DNA DSB rejoining with fast kinetics but has only a small effect

276 sed from 17 to 72% the proportion of DNA DSB rejoining with slow kinetics.

277 ases (11-cis-RDHs), prior to the chromophore rejoining with the visual pigment apo-proteins.

278 a one-base gap in each strand are accurately rejoined, with the gaps being filled by DNA polymerase l