ライフサイエンスコーパス: replicase

1 tly caused by oxidation of the mitochondrial replicase.

2 enzymes are shown to be interacting with the replicase.

3 teracts with nsp3a, a component of the viral replicase.

4 n inhibits the normal functions of the viral replicase.

5 ltransferase and helicase domains of the TMV replicase.

6 nd synthesis, in analogy to Escherichia coli replicase.

7 imulating plus-strand synthesis by the viral replicase.

8 ns (nsPs) representing subunits of the viral replicase.

9 roteins as well as the assembly of the viral replicase.

10 not be used as templates by the tombusvirus replicase.

11 p is a novel component of the purified viral replicase.

12 l) gene fused to a 3' truncated, EPV-encoded replicase.

13 a nucleoside analogue inhibitor and the CoV replicase.

14 echanism during RNA replication by the viral replicase.

15 helper virus (HV) Tobacco mosaic virus (TMV) replicase.

16 e nonstructural proteins that form the viral replicase.

17 that is needed for the assembly of the viral replicase.

18 ciency and limited interference between both replicases.

19 eshold that is compatible with selecting for replicases.

20 tructures which can augment the abundance of replicases.

21 Here we show that protocells can select for replicases.

22 ite of replication and assembly of the viral replicase, activities that are mediated by cis-acting RN

23 We explore various assumptions regarding replicase activity and protocell division.

24 FSBA inactivates HCV replicase activity in a concentration-dependent manner w

25 system can acquire useful functions such as replicase activity or the production of membrane compone

26 reduced, but did not completely block, CHIKV replicase activity were identified: (i) nsP1 tagged at i

27 e equally benefits sequences with or without replicase activity.

28 p92(pol), with consequent inhibition of TBSV replicase activity.

29 eraction, NS5A hyperphosphorylation, and HCV replicase activity.

30 in this huge molecule that are essential to replicase activity.

31 survival, because they can block the cell's replicase and its ability to complete genome duplication

32 These caps harbored the PVX replicase and nonencapsidated vRNA and represented PD-an

33 l proteins (nsPs) function to form the viral replicase and replicate virus RNA.

34 wed that STMV CP promotes binding between HV-replicase and STMV RNA.

35 These viruses encode conserved replicase and structural proteins as well as more divers

36 Steady-state levels of PVX replicase and TGBp2 (which reside in the ER) proteins we

37 hat Polepsilon is the primary leading strand replicase and that Poldelta is restricted to replicating

38 Owing to their mutation-prone replicase and unique genome organization, IAV population

39 a crucial role for the activity of the viral replicase and, thus, the amplification of the viral RNA

40 form for increasing processivity of cellular replicases and for coordinating various cellular pathway

41 s widely used in the expression of RNA virus replicases and represents a potential target for antivir

42 ein, its copurification with the tombusvirus replicase, and its presence in the virus-induced membran

43 s to transport infectious viral RNA, certain replicases, and certain structural proteins to neighbori

44 fectious viral machinery (viral RNA, certain replicases, and certain structural proteins) present in/

45 s of VRCs or VRCs in toto, we isolated viral replicase- and VRC-enriched fractions from TMV-infected

46 cation-competent RNA and expression of viral replicase are uncoupled.

47 Bacterial replicases are complex, tripartite replicative machines.

48 Bacterial replicases are highly processive, yet cycle rapidly duri

49 th mammalian and mosquito cells, novel trans-replicase assays had exceptional sensitivity, with up to

50 ive than wt NS5B in cell-free polymerase and replicase assays.

51 We used an in vitro replicase assembly assay based on yeast cell extract and

52 The cis-elements for replicase assembly can partially overlap with RNA recrui

53 We demonstrate that eEF1A and Hsp70 coopted replicase assembly factors, Vps34 phosphatidylinositol 3

54 demonstrate a critical role for Sac1 in TBSV replicase assembly in a cell-free replicase reconstituti

55 NS2 is not required for NS2-3 processing and replicase assembly.

56 ed the B box, which is also critical for the replicase assembly.

57 in template recruitment into replication and replicase assembly; however, the importance of each of t

58 f the structural protein M and nonstructural replicase-associated proteins nsp1 and nsp13, which are

59 s at the active site of the C-family Pol III replicase at a step that does not require correct base-p

60 en together, these findings suggest that the replicase-ATAF2 interaction suppresses basal host defens

61 In this study, a similar replicase-Aux/IAA interaction affecting disease developm

62 sponding genome counterpart to provide viral replicase (B1+B2+B3/FCP and F1+F2/BCP) resulted in the e

63 on presumably blocks progression of cellular replicases because the N3-methyl group hinders interacti

64 dium, natural selection would not favor such replicases because their presence equally benefits seque

65 tically, RTP4 associates with the flavivirus replicase, binds viral RNA, and suppresses viral genome

66 ORF2p is the L1 replicase, but the role of ORF1p is unknown.

67 mulation and the activity of the tombusvirus replicase by up to fivefold.

68 id composition, nucleotide similarities, and replicase catalytic domain location contributed to phylo

69 ing strand based in its interaction with the replicase chi-subunit.

70 s based on the translational operator of the replicase cistron, a 19 nt fragment (TR).

71 on, indicating that Xrn1 decay and the viral replicase compete to set RNA abundance within infected c

72 the minus strand is synthesized by the viral replicase complex (VRC), which then serves as a template

73 ation proteins and is recruited to the viral replicase complex (VRC).

74 mbrane-bound structures containing the viral replicase complex (VRC).

75 s are replicated by the membrane-bound viral replicase complex (VRC).

76 f several co-opted host factors in the viral replicase complex (VRC).

77 eins and inhibited the assembly of the viral replicase complex and viral RNA synthesis in vitro.

78 on in the localization of genomic RNA to the replicase complex at an early stage of infection.

79 y provide a platform for the assembly of the replicase complex consisting of viral and host proteins.

80 The tombusvirus replicase complex contains heat shock protein 70 (Hsp70)

81 e addition and can be used directly to study replicase complex formation and evolution during infecti

82 II proteins and the viral RNA in tombusvirus replicase complex formation using in vitro, yeast-based,

83 tate the recruitment of GAPDH into the viral replicase complex in the yeast model host.

84 Here, we studied the in vivo primary replicase complex more in-depth to determine the minimum

85 nt metabolic enzyme that is recruited to the replicase complex of Tomato bushy stunt virus (TBSV) and

86 ly from nonplant sources, a fully functional replicase complex of Tomato bushy stunt virus (TBSV).

87 and inner layer made of VP3 that encloses a replicase complex of VP1, VP4, and VP6 and a genome of 1

88 The assembly of the replicase complex required the heat shock protein 70 (Hs

89 pyruvate kinase (PK) directly into the viral replicase complex to boost progeny RNA synthesis.

90 l RNA recruitment, the assembly of the viral replicase complex, and viral RNA synthesis.

91 During the assembly of the membrane-bound replicase complex, the viral RdRp becomes activated thro

92 ary replication but appears to stabilize the replicase complex.

93 translocator during assembly of the primary replicase complex.

94 ication and assembly of the functional viral replicase complex.

95 so necessary for assembly of an active viral replicase complex.

96 to be critical for the assembly of the viral replicase complex.

97 r ORF3 in orchestrating the formation of the replicase complex.

98 To gain insights into the assembly of viral replicase complexes (VRCs) and dissect the roles of vari

99 emonstrate that the in vitro assembled viral replicase complexes (VRCs) in artificial PE vesicles can

100 ruses assemble numerous membrane-bound viral replicase complexes (VRCs) with the help of viral replic

101 dRp) and regulation of the assembly of viral replicase complexes (VRCs).

102 about host factors at the interface between replicase complexes and the host cytoplasm.

103 n, some are recruited to improvise the viral replicase complexes for genome multiplication, and other

104 A viruses, replicate in membrane-bound viral replicase complexes in the cytoplasm of infected cells.

105 They also inhibit formation of functional replicase complexes, but have no activity against prefor

106 es past dUs, even in the presence of stalled replicase complexes, thus providing a mechanism for main

107 tion to facilitate the assembly of the viral replicase complexes, which perform viral RNA replication

108 GuHCl, as well as the components of the MRV replicase complexes.

109 normal viral factories containing functional replicase complexes.

110 hat is proteolytically self cleaved into two replicase components involved in viral RNA replication.

111 and the NS5B polymerase, membrane-bound HCV replicase components.

112 plication by preventing expression of mature replicase components.

113 s with the papain-like protease and putative replicase components: RdRp, methylase and helicase.

114 Bacterial DNA replicases contain multiple subunits that change interac

115 Cellular replicases contain multiprotein ATPases that load slidin

116 s, suggesting that assembly of TBSV and CIRV replicases could take place in the purified ER and mitoc

117 ion 3 in the N-terminal 13aa motif abolished replicase-CP binding.

118 he production of new plus strands, the viral replicase displaces the old plus strand in the dsRNA tem

119 The activity of the mitochondrial replicase, DNA polymerase gamma (Pol gamma) is stimulate

120 components or subassemblies of the bacterial replicase, DNA polymerase III holoenzyme (Pol III HE).

121 Lastly, we demonstrate that the HCV replicase does not colocalize with autophagosomes, sugge

122 due to end-to-end template switching by BMV replicase during (-)-strand synthesis.

123 mavirus (HPV) genomes requires the viral DNA replicase E1.

124 readthrough to regulate expression of their replicase enzymes.

125 ncoded capsid proteins to downstream-encoded replicase enzymes.

126 We found that replicase error rates vary by fork direction, coding sta

127 nses gap filling to modulate affinity of the replicase for the template, we performed photo-cross-lin

128 vivo requirement for two DNA polymerase III replicases for B. subtilis chromosomal replication, both

129 ese co-opted cellular factors in tombusvirus replicase formation.

130 nd as a checkpoint protein that prevents the replicase from advancing in a strand displacement reacti

131 n vitro activity of the purified tombusvirus replicase from gef1Delta yeast was low and that the in v

132 ic viral transcripts expressing an authentic replicase from open reading frame 2 (ORF2) and a second

133 mpounds that inhibit only a single bacterial replicase from those that exhibit broad spectrum potenti

134 By performing assays against replicases from model Gram-negative and Gram-positive ba

135 itness is not due to defective polymerase or replicase function and is more likely to result from the

136 This intramolecular compensation for the HCV replicase function by amino acid changes in different do

137 We demonstrate that PriA blocks replicase function on forks by blocking its binding.

138 that nsp10 is a major regulator of SARS-CoV replicase function.

139 the C-terminal domains are important for HCV replicase function.

140 ive clamp loaders, but tau confers important replicase functions including chaperoning the polymerase

141 2 or 3 is tolerated and that these reporter-replicase fusions can be used to quantitate replication

142 ting the amino-terminal end of the HCoV-NL63 replicase gene and established protease cis-cleavage ass

143 RNA structure that maps to the region of the replicase gene encoding the nonstructural protein 15 sub

144 The results show that the replicase gene has flexibility to accommodate a foreign

145 se results identify a new cistron in the MHV replicase gene locus and show that nsp3 has an essential

146 s a new complementation group within the MHV replicase gene locus.

147 or specific interactions between coronavirus replicase gene products and a cis-acting genomic RNA ele

148 Here, we identify HCoV-NL63 replicase gene products and characterize two viral papai

149 We demonstrate that the replicase gene tolerates the introduction of green fluor

150 between two in-frame NotI sites in the P150 replicase gene, a deletion encompassing nucleotides 1685

151 ernative reading frame overlapping the viral replicase gene.

152 hich are poorly translated relative to CHIKV replicase-generated capped RNAs.

153 We found that the CMV replicase had different fidelity in different environmen

154 hat the clamp loader of the Escherichia coli replicase has the composition DnaX3deltadelta'chipsi.

155 Some sequences - so-called replicases - have enzymatic activity in the sense of enh

156 sed inhibitors and by characterizing them in replicase HCV protease-resistant mutants assay.

157 and that the in vitro assembly of the viral replicase in a cell extract was inhibited by the cytosol

158 itoylation site completely inactivated CHIKV replicase in both human and mosquito cells and was letha

159 drial DNA polymerase (Pol gamma) is the sole replicase in mitochondria.

160 fonyl)benzoyl]adenosine (FSBA) to modify HCV replicase in order to identify the ATP binding site in t

161 Altogether, tombusvirus replicase in the cell-free system showed features remark

162 the presence of non-phosphorylated VP30 or a replicase in the presence of phosphorylated VP30.

163 show that replacement of one of the main DNA replicases in human cells, DNA polymerase delta (Pol del

164 Cellular replicases include three subassemblies: a DNA polymerase

165 s remarkably similar to those of the in vivo replicase, including carrying out a complete cycle of re

166 Using an improved method to map where these replicases incorporate ribonucleotides during replicatio

167 hypothesize that a physical interaction with replicase increases the CP specificity for packaging vir

168 de and mismatch incorporation rates by these replicases influence somatic and germline patterns of va

169 The HCV replicase inhibitor 2'C-methyl adenosine was used to dis

170 convergent synthesis of the prodrugs of HCV replicase inhibitors 1-5 is described.

171 We identified that the STMV CP-HV replicase interaction requires a positively charged resi

172 reversion, illuminate potential multiprotein replicase interactions and coevolution, and support futu

173 nsp6-specific peptide antiserum detected the replicase intermediate p150 (nsp4 to nsp11) and two nsp6

174 The hepatitis C virus (HCV) NS5B replicase is a prime target for the development of direc

175 eaves, we show that brome mosaic virus (BMV) replicase is competent to initiate positive-strand [(+)-

176 a template for (+)RNA synthesis by the viral replicase is facilitated by recruited host DEAD box heli

177 Furthermore, the trimeric replicase is fully functional at a replication fork with

178 Thus, the typical, wild-type HCV replicase is uniquely regulated by lipid peroxidation, p

179 DnaE does not serve as the lagging strand replicase, like DNA polymerase delta in eukaryotes, but

180 cation through proteolytic processing of RNA replicase machinery.

181 e found extensive co-localization of the HCV replicase markers NS5A and double-stranded RNA with Rab5

182 he role of RNA structure and proteins in the replicase-mediated copy-choice mechanism.

183 vious results had suggested that the E. coli replicase might play a role in lesion bypass, but this p

184 s of an Okazaki fragment, the lagging strand replicase must recycle to the next primer at the replica

185 The N terminus of the replicase nonstructural protein 2 (nsp2) of porcine repr

186 has identified putative ts mutations in the replicase nonstructural proteins (nsp's) of these corona

187 ither p150, the precursor polypeptide of the replicase nonstructural proteins nsp4 to nsp10, or the r

188 amino acid substitutions, including 4 in the replicase (nsp1, nsp2, nsp7, and nsp9) and 12 in the str

189 The replicase of all cells is thought to utilize two DNA pol

190 e of insertion and deletion mutations of the replicase of Cucumber mosaic virus (CMV) was determined

191 y exchange with the polymerase III (Pol III) replicase on the beta-clamp and function with DnaB helic

192 ) ions on the in vitro assembly of the viral replicase, on the activity of the viral RNA-dependent RN

193 erase domain generated a polymerase favoring replicase over transcriptase activity, providing strong

194 is fully nested within the ORF of the viral replicase P.

195 The assembled TBSV replicase performed a complete replication cycle, synthe

196 To understand how the mtDNA replicase, Pol gamma, can give rise to elevated mutation

197 accurately bypass this adduct, while Pol III replicase, Pol IV, and Pol V were strongly inhibited.

198 that MGME1 interacts with the mitochondrial replicase PolgA, suggesting that it is a constituent of

199 Pol rate generalizes to B-family eukaryotic replicases, Pols delta and epsilon.

200 P2 acts as a protease that cleaves the viral replicase polyprotein and as a deubiquitinating (DUB) en

201 porter molecules could be expressed from the replicase polyprotein of murine hepatitis virus as fusio

202 s expressed from native locations within the replicase polyprotein of murine hepatitis virus as fusio

203 The processing of replicase polyprotein pp1a in the region of nsp1 to nsp3

204 nonstructural proteins nsp4 to nsp10, or the replicase polyprotein pp1ab to produce nsp12.

205 ine protease domain within the nonstructural replicase polyprotein precursor, is responsible for the

206 gests a link between G3BP proteins and viral replicase polyprotein processing, we propose that G3BP p

207 irst strategy, a mutant was created in which replicase polyprotein translation initiated with nsp3, t

208 s protease activity, which cleaves the viral replicase polyprotein, and also DUB activity (deconjugat

209 ke protease (PLP), which processes the viral replicase polyprotein, has deubiquitinating (DUB) activi

210 delay in proteolytic processing of the viral replicase polyprotein.

211 LPs), PLP1 and PLP2, which process the viral replicase polyprotein.

212 lication by cleaving a site within the viral replicase polyproteins and also removes ubiquitin from c

213 Coronavirus replicase polyproteins are translated from the genomic p

214 ke protease (PL(pro)) that cleaves the viral replicase polyproteins at three sites releasing non-stru

215 positive-stranded RNA viruses) express their replicase polyproteins pp1a and pp1ab from two long ORFs

216 Coronaviruses encode large replicase polyproteins which are proteolytically process

217 ole in the proteolytic cleavage of the PRRSV replicase polyproteins.

218 al protein P1 (nsP1) and nsP2 regions of the replicase precursor polyprotein (1/2 site), while a diff

219 lity of specific phenotypes; error-prone DNA replicases produce bursts of variability in times of str

220 The identification of the replicase products and characterization of HCoV-NL63 PLP

221 We found that HCoV-NL63 replicase products can be detected at 24 h postinfection

222 Mouse hepatitis virus (MHV) replicase products nsp3, nsp4, and nsp6 are predicted to

223 ent with its proposed role in a multiprotein replicase-proofreading complex.

224 we recently showed that multifunctional FHV replicase protein A induces viral RNA template recruitme

225 evaluating the molecular interaction between replicase protein and CP using a FHV-Nicotiana benthamia

226 The data suggest that replicase protein interactions directly or indirectly re

227 The nsp2 replicase protein of porcine reproductive and respirator

228 the Tobacco mosaic virus (TMV) 126-/183-kDa replicase protein(s) and the Arabidopsis thaliana NAC do

229 noprecipitation assays demonstrated that FHV replicase (protein A) and CP physically interact at the

230 e, we found that during virus infection, the replicase proteins containing the MAC and PLP2 mutations

231 could define the expression and targeting of replicase proteins during infection in live cells.

232 s could define the targeting and activity of replicase proteins during infection.

233 Thus, the timing and targeting of native replicase proteins expressed in real time from native lo

234 The replicase proteins form membranous compartments in cells

235 ionally, live-imaging studies of coronavirus replicase proteins have used fluorescent reporter molecu

236 g exonuclease (nsp14-ExoN), as well as other replicase proteins involved in regulation of fidelity.

237 HM.WU structural protein M and nonstructural replicase proteins nsp1 and nsp13 are essential for live

238 NA) of Cucumber Mosaic Virus is dependent on replicase proteins of helper virus (HV).

239 The capsid and replicase proteins of PCV3 are only 37% and 55% identica

240 We also show that coevolution between replicase proteins over long-term passage partially comp

241 Multiple small circular DNA genomes encoding replicase proteins plus two highly divergent members of

242 olutionary relationships between coronavirus replicase proteins, and identify potential mechanisms fo

243 used fluorescent reporter molecules fused to replicase proteins, but expressed from nonnative locatio

244 membrane fractions, which contain the viral replicase proteins, in cells with replicating HCV.

245 resulted in aberrant agglomeration of viral replicase proteins, including NS5A, NS5B, and NS3.

246 These create a platform to concentrate replicase proteins, virus genomes and host proteins requ

247 rotein or firefly luciferase as fusions with replicase proteins.

248 the RC and alters the colocalization of HCV replicase proteins.

249 tiple point mutations in their nonstructural replicase proteins.

250 that are important for the first step of the replicase reaction: the ATP-dependent formation of an in

251 fector on TBSV replication using a cell-free replicase reconstitution assay.

252 c1 in TBSV replicase assembly in a cell-free replicase reconstitution assay.

253 igher reporter expression in the presence of replicase relative to background.

254 The formation of the TBSV replicase required two purified recombinant TBSV replica

255 GUV-based reconstitution of the TBSV replicase revealed the need for a complex mixture of pho

256 An RNA replicase ribozyme has long been sought by chemists inte

257 ates, are necessary; accordingly the minimal replicase ribozyme may have possessed restriction functi

258 However, how such replicase ribozymes emerged from the pools of short RNA

259 stem provides a valuable tool to study CHIKV replicase, RNA replication, and virus-host interactions.

260 ion of the yeast extract, in which the viral replicase-RNA complex became RNase- and proteinase-resis

261 n a membraneous fraction, in which the viral replicase-RNA complex was RNase and protease resistant b

262 DNA replicases routinely stall at lesions encountered on the

263 Although it is well documented that archaeal replicases specifically arrest at deoxyuracils (dUs) due

264 repriming replication restart downstream of replicase stalling lesions and structures.

265 that the SARS-CoV proteome contains several replicase, structural, and accessory proteins that antag

266 ed by a protein factor, specifically a PRRSV replicase subunit (nsp1beta).

267 for a trans-acting protein factor, the viral replicase subunit nsp1beta.

268 tions were mapped to the amino terminus of a replicase subunit, nonstructural protein 3 (nsp3).

269 [(-)ssRNA] viruses carry at infection an RNA replicase that makes multiple translation-competent copi

270 hogens, the helicase domain (p50) of the TMV replicase, the avirulence gene of N, was linked to synth

271 nisms that dictate the activity of the viral replicase, thereby paving the way for future studies.

272 exes, but have no activity against preformed replicase, thereby resulting in slow shut-off of viral R

273 The ability of the TMV replicase to interact with Aux/IAA proteins from diverse

274 but not completely block the ability of its replicase to synthesize viral RNAs.

275 there are major differences among the viral replicases to generate and maintain interviral recombina

276 The in vitro assembly of the replicase took place in the membraneous fraction of the

277 to tether the genome to the newly translated replicase-transcriptase complex at a very early stage of

278 N protein associates with a component of the replicase-transcriptase complex, nonstructural protein 3

279 tween N and the largest subunit of the viral replicase-transcriptase complex, nonstructural protein 3

280 The largest component of the coronavirus replicase-transcriptase complex, nsp3, contains multiple

281 onstructural protein 15 subunit of the viral replicase-transcriptase complex.

282 se hepatitis virus (MHV) is carried out by a replicase-transcriptase composed of 16 nonstructural pro

283 s of RNA oligonucleotides that encompass the replicase translational operator stem-loop of the RNA ba

284 Pol delta, like bacterial replicases, undergoes collision release upon completing

285 ns from the NS3-NS5B polyprotein to create a replicase unit for replication of its genome.

286 econstituted Tomato bushy stunt virus (TBSV) replicase using artificial giant unilamellar vesicles (G

287 , in contrast to related alphaviruses, CHIKV replicase was completely inactivated by mutations preven

288 n insights into the functions of a viral RNA replicase, we have assembled in vitro and entirely from

289 re of the (-)RNA in the membrane-bound viral replicase, we performed complete RNA replication of Toma

290 tions in the structural proteins but not the replicase were responsible for the establishment of pers

291 exhibit much lower fidelity than the cell's replicase when copying normal DNA, which results in a dr

292 in the in vitro activity of the tombusvirus replicase when isolated from APB-treated yeast.

293 unexpected assembly of the mitochondrial DNA replicase where the catalytic subunit Pol gammaA interac

294 of several cellular proteins into the viral replicase, which otherwise play proviral roles in replic

295 which one of the FluPol molecules acts as a replicase while the other initiates the assembly of the

296 Pol III holoenzyme is the cell's main replicase, while pol I is responsible for the maturation

297 ruses [(+)RNA viruses] is performed by viral replicases, whose function is affected by many cellular

298 nants generated by template switching of BMV replicase with a nascent UTR from WT RNA1 or RNA2 during

299 Immunofluorescence experiments with CHIKV replicase with manipulated processing indicate that the

300 Complementation with homologous replicase (with respect to CP) failed to enhance packagi