ライフサイエンスコーパス: replication checkpoint

1 -MCM2 and are responsible for regulating DNA replication checkpoint.

2 protein Mrc1 in activating Rad53 in the DNA replication checkpoint.

3 rks stalled by hydroxyurea that activate the replication checkpoint.

4 n of ATR and Chk1 and inhibited the ATR-Chk1 replication checkpoint.

5 on on DnaA through activation of the Sda DNA replication checkpoint.

6 racting protein that participates in the DNA replication checkpoint.

7 efficient and timely termination of the DNA replication checkpoint.

8 ondensation in cells, indicating a defect in replication checkpoint.

9 Here we describe the roles of Sap1 in the replication checkpoint.

10 ckpoint kinase 2) is a major effector of the replication checkpoint.

11 the Chk1 damage checkpoint but not the Cds1 replication checkpoint.

12 es regulation of late origins by the intra-S replication checkpoint.

13 by an entirely distinct checkpoint, the DNA replication checkpoint.

14 lled by both Cyclin B limitation and the DNA-replication checkpoint.

15 lting from stalled forks that escape the ATR replication checkpoint.

16 ivity of Rqh1 plays an important role in the replication checkpoint.

17 indicating that ATR is required for the DNA replication checkpoint.

18 both leads to synergistic disruption of the replication checkpoint.

19 kd expression there was a marked loss of the replication checkpoint.

20 een chromatin structure and induction of the replication checkpoint.

21 t component of the signal that activates the replication checkpoint.

22 ells, it was unable to restore fully the DNA replication checkpoint.

23 ds1, serves an essential function in the DNA replication checkpoint.

24 of DNA damage and may play a role in the DNA replication checkpoint.

25 ibuted to a mitotic delay imposed by the S-M replication checkpoint.

26 e that regulates the DNA damage response and replication checkpoint.

27 at its deficiency activates a SOG1-dependent replication checkpoint.

28 er of Cdc25p (OPcdc25(+)), which lacks a DNA replication checkpoint.

29 Cds1 and Chk1 appear to jointly enforce the replication checkpoint.

30 onsequently, for activation of a meiotic DNA replication checkpoint.

31 sly been believed to be involved only in the replication checkpoint.

32 hat phosphorylate Cdc2, are regulated by the replication checkpoint.

33 ive in the DNA damage checkpoint but not the replication checkpoint.

34 bundance of Cdc25 are unconnected to the DNA replication checkpoint.

35 is also maintained in cells arrested at the replication checkpoint.

36 aintained in wild-type cells arrested at the replication checkpoint.

37 se to stimulate Mec1 kinase and initiate the replication checkpoint.

38 ells, Cds1 is the effector kinase of the DNA replication checkpoint.

39 replication fork stalling and activated the replication checkpoint.

40 oading but is separable from its role in the replication checkpoint.

41 ge accumulation caused by defects in the DNA replication checkpoint.

42 the minimal signal required to activate the replication checkpoint.

43 uppressed by ATR, a central regulator of the replication checkpoint.

44 ng a mechanism for fork stabilization by the replication checkpoint.

45 oth cellular DNA damage response and the DNA replication checkpoint.

46 is a major player in the control of the DNA replication checkpoint.

47 te to regulation of TopBP1 stability and DNA replication checkpoint.

48 el that Dpb11 couples DNA replication to the replication checkpoint.

49 plays a critical role in the control of DNA replication checkpoint.

50 rerequisite for the subsequent activation of replication checkpoint.

51 oteins, leading to the activation of the DNA replication checkpoint.

52 te switching-depend on the activation of the replication checkpoint.

53 and Chk1 are two key protein kinases in the replication checkpoint.

54 noise immunity, properties required for the replication checkpoint.

55 nal transducers in eukaryotic DNA damage and replication checkpoints.

56 use damage that activates the DNA-damage and replication checkpoints.

57 ng the activation of both the DNA damage and replication checkpoints.

58 solvases, with intriguing connections to DNA replication checkpoints.

59 cells and in cells subject to DNA damage or replication checkpoints.

60 cks S-phase completion but not activation of replication checkpoints.

61 ial component of both the DNA damage and DNA replication checkpoints.

62 tein kinases required for the DNA damage and replication checkpoints.

63 logenetic conservation of the DNA damage and replication checkpoints.

64 ich normally activate the DNA damage and DNA replication checkpoints.

65 network that dysregulates cell-cycle and DNA replication checkpoints.

66 nuclear-to-cytoplasmic ratio and on the DNA replication checkpoints.

67 cytoplasmic ratio, but not requiring the DNA replication checkpoints.

68 equired for activation of the DNA damage and replication checkpoints.

69 Fission yeast Mrc1 (mediator of replication checkpoint 1) is an adaptor checkpoint prote

70 stress results in the activation of the DNA replication checkpoint, a signaling cascade whose centra

71 ed mitosis, Hsl7 overexpression overrode the replication checkpoint, accelerating Wee1 destruction.

72 The DNA damage/replication checkpoints act by sensing the presence of d

73 gest an antagonistic interaction between DNA replication checkpoint activation and Cdk1-CycB activity

74 is independent of its known functions in DNA replication checkpoint activation and replisome speed co

75 Replication checkpoint activation disrupted Hsl7-Wee1 in

76 energy controller, determines a fast mode of replication checkpoint activation through a redox mechan

77 egulation of both normal DNA replication and replication checkpoint activation through the novel phos

78 The replisome is important for DNA replication checkpoint activation, but how specific comp

79 tial blastoderm stage cell-cycle delays, DNA replication checkpoint activation, cellularization, and

80 Chk1 and cell-cycle arrest, consistent with replication checkpoint activation.

81 lian DNA helicase and has been implicated in replication checkpoint activation.

82 lation and allowed late-origin firing during replication checkpoint activation.

83 We conclude that normal S phase and replication checkpoint activities are important timers o

84 This replication checkpoint acts by down-regulating the activ

85 n yeast Schizosaccharomyces pombe, Mrc1 is a replication checkpoint adaptor protein that allows the s

86 This two-stage activation mechanism for the replication checkpoint allows for rapid activation with

87 ditions that activate the DNA damage and DNA replication checkpoints also activated the spindle check

88 are required for both the DNA damage and the replication checkpoints, an exception being chk1 .

89 Rad53 kinase is a central effector of the replication checkpoint and both binds to and phosphoryla

90 man TopBP1 participates in the activation of replication checkpoint and DNA damage checkpoints, follo

91 Chk1 plays a key role in regulating the replication checkpoint and DNA damage response.

92 mechanism of how the replisome regulates the replication checkpoint and genomic stability.

93 SpCds1 functions in the DNA replication checkpoint and in the S phase DNA damage che

94 arrest appeared not to be related to the DNA replication checkpoint and not to be mediated through in

95 cts have demonstrated that activation of the replication checkpoint and phosphorylation of the Chk1 k

96 se data establish Hsl7 as a component of the replication checkpoint and reveal that similar cell cycl

97 optotic sensitivity to inhibitors of the DNA replication checkpoint and suggesting it as a candidate

98 phorylation of Cdc2 is essential for the DNA replication checkpoint and suggests that Cdc25, and/or o

99 ions redundantly with the kinase Cds1 at the replication checkpoint and that both kinases phosphoryla

100 y network integrating NF-kappaB with the DNA-replication checkpoint and the expression of critical re

101 hereby contributing to the activation of the replication checkpoint and the stabilization of replicat

102 lay important roles in the activation of the replication checkpoint and the stabilization of stalled

103 heckpoint response, but not DNA replication, replication checkpoint, and cell cycle progression.

104 essential roles in DNA metabolism, including replication, checkpoint, and repair.

105 DNA replication machinery interact with the replication checkpoint apparatus.

106 Mechanisms controlling DNA replication and replication checkpoint are critical for the maintenance

107 lowed replication fork restart, although DNA replication checkpoints are functional.

108 d PTEN and DNA-PK as essential regulators of replication checkpoint arrest in response to AZD1775 and

109 These mutations also impair the replication checkpoint arrest.

110 Furthermore, Cdc2 isolated from replication checkpoint-arrested cells can be activated i

111 ivity, CHES1 had no measurable effect on the replication checkpoint as assayed by hydroxyurea sensiti

112 DPB11, cut5, has been implicated in the DNA replication checkpoint as has the POL2 gene with which D

113 The DNA damage and DNA replication checkpoints block anaphase in response to DN

114 Further, replication checkpoint blockade via small-molecule inhib

115 ncy does not alter the activation of the DNA replication checkpoint but rather affects the execution

116 activity and cell survival in the endogenous replication checkpoint but that Ddc1 is absolutely requi

117 lity to activate Mec1 are proficient for the replication checkpoint, but they are compromised for the

118 replication ensemble and plays a role in the replication checkpoint by directly associating with repl

119 Therefore, the DNA replication checkpoint can prevent mitosis by suppressin

120 d postreplicative repair, and DNA damage and replication checkpoints caused growth defects or lethali

121 S. pombe Ddb1 is functionally linked to the replication checkpoint control gene cds1.

122 y a potentially critical role for Claspin in replication checkpoint control in mammalian cells.

123 ternal cell-cycle programme, and disrupt DNA-replication checkpoint control of cell-cycle progression

124 Here, we show that a DNA replication checkpoint control operates during meiosis i

125 for DNA replication and have been linked to replication checkpoint control.

126 ensure the proper cell cycle progression and replication checkpoint control.

127 hat ETAA1 is a new ATR activator involved in replication checkpoint control.

128 t Drc1 is required for DNA synthesis and S-M replication checkpoint control.

129 P1 is a key mediator protein involved in DNA replication checkpoint control.

130 PA2 functionally interact and participate in replication checkpoint control.

131 t that BACH1 has an unexpected early role in replication checkpoint control.

132 es, TopBP1 may be involved in DNA damage and replication checkpoint controls.

133 of which are required for DNA damage and/or replication checkpoint controls.

134 The DNA replication checkpoint couples the onset of mitosis with

135 encodes Checkpoint kinase 1 (Chk1), are DNA-replication-checkpoint defective and fail to cellularize

136 show that mnk grp double-mutant embryos are replication-checkpoint defective but cellularize, gastru

137 These data suggest that replication checkpoint defects may result indirectly fro

138 We have found that a DNA replication checkpoint dependent on the ATM homolog MEC1

139 pin is phosphorylated at the MBT at both DNA replication checkpoint-dependent and -independent sites.

140 Finally, the DNA replication checkpoint did not contribute to the arrests

141 The DNA replication checkpoint (DRC) monitors and responds to st

142 es or genes involved in DNA replication, DNA-replication checkpoint (DRC) signaling, and oxidative st

143 They are required to mediate the DNA replication checkpoint (DRC), the stable pausing of fork

144 Cds1 protein kinase are required for the DNA replication checkpoint during meiosis, with Cds1 playing

145 in preventing spontaneous activation of the replication checkpoint during normal cell cycles.

146 The DNA replication checkpoint ensures that mitosis is not initi

147 The ATR replication checkpoint ensures that stalled forks remain

148 Effectors of the replication checkpoint evoked by hydroxyurea (HU) are un

149 nction of checkpoint regulators, such as the replication checkpoint factors Tof1 and Rad53.

150 This study suggests that inhibition of replication checkpoint function can suppress skin carcin

151 Therefore, the replication checkpoint function of Rqh1 is highly conser

152 This defect in replication checkpoint function was reversed after the a

153 show that, contrary to current models of DNA replication checkpoint function, replication proteins re

154 Tel1, the ortholog of human ATM, has a minor replication checkpoint function.

155 romosomal fragile sites in cells lacking the replication checkpoint gene ATR.

156 In addition, vegetative replication checkpoint genes are not required for the ar

157 syncytial cell-cycle timing or the embryonic replication checkpoint, however, suggesting that Mei-41-

158 We identified a Mec1/ATR- and Dbf4-dependent replication checkpoint in budding yeast that prevents th

159 enomic DNA replication and abrogated the DNA replication checkpoint in cell-free extracts.

160 ll cycle transcriptional program and the DNA replication checkpoint in fission yeast.

161 the role of human Claspin in the DNA damage/replication checkpoint in mammalian cells.

162 We report that the DNA replication checkpoint in S. pombe is abrogated in cells

163 The DNA replication checkpoint in Schizosaccharomyces pombe cont

164 ogue of ATR and the sensor kinase of the DNA replication checkpoint in Schizosaccharomyces pombe Unde

165 og of Chk2 and the major effector of the DNA replication checkpoint in Schizosaccharomyces pombe.

166 have implicated the Saccharomyces cerevisiae replication checkpoint in the suppression of spontaneous

167 proteins to Cdc25 in vitro and disrupts the replication checkpoint in vivo.

168 Pin1 was found to be required for the DNA replication checkpoint in Xenopus laevis.

169 Initiation of the DNA replication checkpoint in yeast is mainly mediated by Me

170 1, a checkpoint protein required for the DNA replication checkpoint in yeast.

171 The G(2) DNA damage and DNA replication checkpoints in many organisms act through th

172 an essential component of the DNA damage and replication checkpoints in the fission yeast Schizosacch

173 1 plays a crucial role in the DNA damage and replication checkpoints in vertebrates and may therefore

174 resence of unreplicated DNA (that is, in the replication checkpoint) in fission yeast.

175 emonstrated that in Saccharomyces cerevisiae replication, checkpoint inactivation via a mec1 mutation

176 cycle 7 and cycle 10, this extension is DNA-replication checkpoint independent, but correlates with

177 prevented double-strand break formation in a replication-checkpoint-independent manner, and delaying

178 from the Ppp5-deficient mice maintain normal replication checkpoint induced by hydroxyurea, Ppp5-defi

179 To determine the effect of replication checkpoint inhibition on carcinogenesis, we

180 y key cell death-initiating events that link replication checkpoint inhibition to antitumor response

181 This study demonstrates that replication checkpoint inhibitors can kill AML cells thr

182 The DNA replication checkpoint inhibits mitosis in cells that ar

183 The DNA replication checkpoint is a complex signal transduction

184 The replication checkpoint is a dedicated sensor-response sy

185 evel zygotic transcription is initiated, the replication checkpoint is activated and the cell cycle s

186 We report that the replication checkpoint is activated in two stages.

187 The replication checkpoint is activated when replication for

188 We previously showed that when the DNA replication checkpoint is activated, the repressor Yox1

189 As a result, the replication checkpoint is activated.

190 The DNA replication checkpoint is crucial for maintaining genome

191 In fission yeast, the replication checkpoint is enforced by the kinase Cds1 (h

192 The replication checkpoint is essential for accurate DNA rep

193 tely replicated DNA, indicating that the DNA replication checkpoint is inactive under these condition

194 1-1 and for the activation of XChk1 when the replication checkpoint is induced by aphidicolin.

195 We conclude that the Sda replication checkpoint is required to delay the initiati

196 accharomyces pombe the major effector of the replication checkpoint is the protein kinase Cds1.

197 We propose that while the DNA replication checkpoint is unlikely to regulate the assem

198 a telangiectasia and Rad3-related (ATR) is a replication checkpoint kinase activated by DNA stresses

199 ar stresses, including inhibition of the DNA replication checkpoint kinase ATR (ataxia telangiectasia

200 We demonstrate that the replication checkpoint kinase ATR, but not ATM, is criti

201 at Sap1 is involved in the activation of the replication checkpoint kinase Cds1 and that sap1 mutant

202 Upon replication fork arrest, the replication checkpoint kinase Cds1 is stimulated to pres

203 The replication checkpoint kinase Cds1 preserves genome inte

204 val of fork arrest and for activation of the replication checkpoint kinase Cds1.

205 ddb1 strain to an increased activity of the replication checkpoint kinase Cds1.

206 s on the 14-3-3 chaperone Rad24p and the DNA replication checkpoint kinase Cds1p.

207 But activation of the replication checkpoint kinase Chk2 is unaffected, defini

208 tion of the Cdc10 subunit of MBF by the Cds1 replication-checkpoint kinase.

209 In vivo, DNA synthesis stalling activates replication checkpoint kinases, which act to preserve th

210 homologous recombination (HR) DNA repair and replication checkpoints lead to retention of DSBs that p

211 We conclude that the DNA-replication checkpoint maintains genome integrity during

212 stress in Saccharomyces cerevisiae, the DNA replication checkpoint maintains replication fork stabil

213 In the endogenous replication checkpoint, Mec1 phosphorylation of Rad53 re

214 on of Cdc2 on tyrosine-15 is not part of the replication checkpoint mechanism.

215 provide some insight into the DNA damage and replication checkpoint mechanisms of HRad17.

216 cation initiation and preferably impairs DNA replication checkpoint mediated by ATR and CHK1.

217 strate for the first time a link between the replication checkpoint mediated by ATR/Chk1 and the tran

218 yeast carrying deletions of both ISC1 and a replication checkpoint mediator gene including MRC1, TOF

219 f DNA chain elongation and activation of the replication checkpoint might be responsible for the UVC-

220 The replication-checkpoint mimic requires colocalization of

221 Therefore we constructed an in vivo replication-checkpoint mimic that recapitulates Mec1-dep

222 Recent studies indicate that replication checkpoint modulators (RCMs) such as inhibit

223 d lethality, because it not only impairs DNA replication checkpoint more profoundly than inhibition o

224 cells tolerate deletions of mediator of the replication checkpoint (MRC) 1 (mrc1Delta) and radiation

225 Replication checkpoint mutant cds1Delta shows increased

226 Yeast replication checkpoint mutants lose viability following

227 We show that CtrA also mediates a DNA replication checkpoint of cell division by regulating th

228 activation of the sensor kinase Rad3 in the replication checkpoint of fission yeast may involve a di

229 genes that function specifically in the DNA replication checkpoint pathway, we searched for high-cop

230 lex and Exo1 contribute to activation of the replication checkpoint pathway.

231 T1 gene as an effector of the DNA damage and replication checkpoint pathway.

232 h is required for activation of Chk1 and the replication checkpoint pathway.

233 oteins participate in the DNA damage and DNA replication checkpoint pathways and are critical to main

234 is a signal transducer in the DNA damage and replication checkpoint pathways and functions downstream

235 red for the activation of DNA damage and DNA replication checkpoint pathways, and that is implicated

236 sential protein kinase in DNA damage and DNA replication checkpoint pathways.

237 Our data reveal how the replication checkpoint preserves genome integrity, provi

238 rom the fork stalled at the lesion site, the replication checkpoint presumably coordinates the action

239 In eukaryotes, the DNA replication checkpoint prevents entry into mitosis when

240 tion forks stall and restart and how the DNA replication checkpoint prevents irreversible fork collap

241 In yeast, Mec1 protein kinase-mediated replication checkpoint prevents the breakdown of replica

242 that Chk1, a component of the DNA damage and replication checkpoints, protects vertebrate cells again

243 Here, we show that Rad17, a replication checkpoint protein, is required for the earl

244 ndicate that, like TopBP1, Treslin is a dual replication/checkpoint protein that directly participate

245 somerase IIbeta-binding protein 1) is a dual replication/checkpoint protein.

246 eplication forks that are then stabilized by replication checkpoint proteins, whereas CAF-I defects l

247 n asf1 mutants have a strong requirement for replication checkpoint proteins, whereas normal S-phase

248 fission yeast Schizosaccharomyces pombe, the replication checkpoint regulates the entire G(1)/S trans

249 is resistance does not rely on the essential replication checkpoint regulator WEE1 but could be block

250 The DNA replication checkpoint remains intact in ATR knockout ce

251 er, whether mutp53 directly perturbs the DNA replication checkpoint remains unclear.

252 has previously shown that activation of the replication checkpoint requires the initiation of DNA sy

253 yeast independent of both the Mrc1-mediated replication checkpoint response and the Psk1-Mrc1 oxidat

254 ulation of Rad60 is an important part of the replication checkpoint response controlled by Cds1.

255 iotic response to DNA damage due to a failed replication checkpoint response differs substantially fr

256 e transcriptional program is part of the DNA replication checkpoint response in human cells and estab

257 for the ATR-dependent activation of the DNA replication checkpoint response in Xenopus and human cel

258 that ObgE defines a pathway analogous to the replication checkpoint response of eukaryotes and acts i

259 We found that during recovery from the DNA replication checkpoint response, Claspin is degraded in

260 ts suggest that WRN and ATM participate in a replication checkpoint response, in which WRN facilitate

261 contributes to two key functions of the DNA replication checkpoint response, namely, preventing geno

262 mp functioning in the S phase DNA damage and replication checkpoint response, upon treatment with DNA

263 n of Claspin results in termination of a DNA replication checkpoint response.

264 are involved in a common pathway in the DNA replication checkpoint response.

265 ning multiprotein complexes participate in a replication checkpoint response.

266 his distinct class of G1/S genes for the DNA replication checkpoint response.

267 tion protein Mcm2 during both DNA damage and replication checkpoint responses in Xenopus egg extracts

268 s a replisome component that participates in replication checkpoint responses to genotoxic stress, it

269 ATM and ATR, are required for DNA damage and replication checkpoint responses.

270 damage-induced G(2)/M arrest and a subset of replication checkpoint responses.

271 The DNA replication checkpoint restrains fork progression, which

272 During the replication checkpoint, Saccharomyces cerevisiae Dbf4 is

273 rks in a configuration that is recognized by replication checkpoint sensors.

274 nopus ATR (XATR) and Hus1 (Xhus1) as the DNA replication checkpoint sensors.

275 S1 branches of the intra-S checkpoint or the replication checkpoint showed synergistic interactions w

276 thought to act as a specific mediator of the replication checkpoint signal that activates the effecto

277 ication initiation is required to induce the replication checkpoint signal.

278 ut is accompanied by rapid activation of DNA replication checkpoint signalling, and active DNA replic

279 that Cdc2 is in an activated state during a replication checkpoint, suggesting that phosphorylation

280 epair and recover normally from the G2/M and replication checkpoints, suggesting a specific function

281 icating MBF as a target of the budding yeast replication checkpoint, suggests that checkpoint regulat

282 kpoint-specific transcriptional program, the replication checkpoint targets MBF to maintain the norma

283 Swi1 is also needed for the replication checkpoint that arrests division in response

284 within the yxcC gene and at or near the left replication checkpoint that is activated under stringent

285 cell cycle checkpoint responses and the DNA replication checkpoint that prevents mitosis before the

286 protein kinase Cds1 is activated by the S-M replication checkpoint that prevents mitosis when DNA is

287 lls lacking either the DNA damage or the DNA replication checkpoints, the spindle checkpoint contribu

288 clb6 are unable to activate the meiotic DNA replication checkpoint, they do possess an intact DNA da

289 after activation of either the DNA damage or replication checkpoints; this is accompanied by a slight

290 (MBT) is a requirement for a functional DNA replication checkpoint to coordinate cell-cycle remodeli

291 However, the role and mechanism of the replication checkpoint transcriptional response in human

292 The DNA replication checkpoint transcriptionally upregulates gen

293 el2 mutant, the phospho-signaling in the DNA replication checkpoint was almost completely eliminated.

294 Loss of both the DNA damage and replication checkpoints was observed in fission yeast ce

295 on and is also primarily targeted by the DNA replication checkpoint when replication stalls.

296 se results demonstrate that a Chk1-dependent replication checkpoint which slows S phase progression c

297 what role these Mec1 activators play in the replication checkpoint, which recognizes stalled replica

298 ction pathway involved in the DNA damage and replication checkpoints, while Cdc7-Dbf4 kinase (DDK) is

299 Dna2 shows partial redundancy for the replication checkpoint with checkpoint initiators 9-1-1

300 or sufficient uncoupling to activate the DNA replication checkpoint with excessive destabilizing sing