ライフサイエンスコーパス: replication initiation

1 e.g., sterol biosynthesis) or not (e.g., DNA replication initiation).

2 genes cause disruption of the origin of DNA replication initiation.

3 -phase and define potential locations of DNA replication initiation.

4 cells imparts a rapid and complete block to replication initiation.

5 helicases that are central to bidirectional replication initiation.

6 changes, which are likely incompatible with replication initiation.

7 reaction that exhibits hallmarks of cellular replication initiation.

8 orylation, each previously shown to regulate replication initiation.

9 parapsilosis also employs recombination for replication initiation.

10 ediate chromosome segregation independent of replication initiation.

11 our understanding of mechanisms that control replication initiation.

12 bly of the GINS complex but is essential for replication initiation.

13 repair in addition to its essential role in replication initiation.

14 hereupon a dihexamer forms, resulting in DNA replication initiation.

15 to E2F1, which results in the termination of replication initiation.

16 ng partners on chromatin and facilitates DNA replication initiation.

17 ents lead to reverse transcription and viral replication initiation.

18 lay in establishing the genomic landscape of replication initiation.

19 es adjacent to one side of the major site of replication initiation.

20 45 and GINS helicase-activating proteins and replication initiation.

21 pBP1-treslin interaction is critical for DNA replication initiation.

22 ity may be a common mechanism for regulating replication initiation.

23 o the molecular mechanisms of eukaryotic DNA replication initiation.

24 by cyclin-dependent kinases is essential for replication initiation.

25 ucidated concerning their functional role in replication initiation.

26 omplexes of these proteins are necessary for replication initiation.

27 lows origin licensing and prevents premature replication initiation.

28 e dnaA promoter to control the timing of DNA replication initiation.

29 ggesting that LPS phosphorylation stimulates replication initiation.

30 tin structure plays an important role in DNA replication initiation.

31 protein that may act as an activator of DNA replication initiation.

32 ng the viability of mutants defective in DNA replication initiation.

33 effects of a primary activity regulating DNA replication initiation.

34 is the focus of several pathways to control replication initiation.

35 allows Cdc45 loading and derepression of DNA replication initiation.

36 of ParA halts segregation without affecting replication initiation.

37 for origin licensing, a prerequisite for DNA replication initiation.

38 BNA1 at sites of active transcription or DNA replication initiation.

39 e oriC to locally melt the DNA duplex during replication initiation.

40 tional importance of OGRE/G4 elements in DNA replication initiation.

41 ., constant growth increments) that start at replication initiation.

42 case is the committed step in eukaryotic DNA replication initiation.

43 recruitment during reactivation to preclude replication initiation.

44 g cell physiology, separate from its role in replication initiation.

45 ycle exit by functioning at a step after DNA replication initiation.

46 Mcm10 are factors that are also required for replication initiation.

47 possibly be independent from its role in DNA replication initiation.

48 s and epigenetic modifications in regulating replication initiation.

49 CD2 reduces the number of potential sites of replication initiation.

50 e a common G-rich motif and exhibit elevated replication initiation.

51 ive an analytical expression for the rate of replication initiation.

52 ouble hexamer that facilitates bidirectional replication initiation.

53 ts of the pre-replication complex during DNA replication initiation.

54 howed that AZ-27 inhibited transcription and replication initiation.

55 synthesis and shortens S phase by increasing replication initiation.

56 scuss here the molecular choreography of DNA replication initiation across the three domains of life,

57 c2 or Cdt1 suffice to recruit all downstream replication initiation activities to a prospective origi

58 se cyclin-dependent kinases (CDKs) stimulate replication initiation and accelerate progression throug

59 etabolic complex is enriched at sites of DNA replication initiation and associated with proliferating

60 DDK plays essential roles in DNA replication initiation and checkpoint activation.

61 We used RNAi against Cdc7 kinase to inhibit replication initiation and dissect this checkpoint in fi

62 e helicase 4 (RECQL4) plays crucial roles in replication initiation and DNA repair; however, the cont

63 This enzyme is implicated in replication initiation and DNA topology restoration and

64 se, consistent with the observation that DNA replication initiation and elongation are impaired in so

65 chanism by which a checkpoint kinase targets replication initiation and elongation complexes, which m

66 To investigate the contribution of DNA replication initiation and elongation to the intra-S-pha

67 we establish that RbN directly inhibits DNA replication initiation and elongation using a bipartite

68 ates WEE1-MCM2 signaling, which inhibits DNA replication initiation and enables BRCA1-deficient cells

69 dimer is required both for activation of DNA replication initiation and for interaction with Spo0J.

70 multiple processes, including inhibition of replication initiation and fork stabilization.

71 ct relationship between sites of reduced DNA replication initiation and genetic damage.

72 phed mechanisms, often through the action of replication initiation and helicase-loader proteins.

73 lication stress is caused by deregulation of replication initiation and increased interference betwee

74 s in plasmid origins and participate both in replication initiation and its control.

75 Here, we review our current understanding of replication initiation and its regulation, describe mech

76 rates by primase may influence regulation of replication initiation and Okazaki fragments processing.

77 lays a key role in determining the timing of replication initiation and origin usage.

78 aA, DnaD, and DnaB to help properly regulate replication initiation and perhaps are analogous to the

79 mage, BRCA1 deficiency markedly enhances DNA replication initiation and preferably impairs DNA replic

80 he importance of protein acetylation for DNA replication initiation and progression, and suggests tha

81 of the response regulator CtrA represses DNA replication initiation and regulates the transcription o

82 se relationship between the durations of DNA replication initiation and replication.

83 h chloramphenicol relieves inhibition of DNA replication initiation and RNA synthesis in HipA-arreste

84 A genome-scale correlation between DNA replication initiation and RNA transcription, which is e

85 data help explain how CDK activity controls replication initiation and suggest that replication prog

86 These data suggest that replication initiation and termination are coupled to tr

87 ntified and quantitatively compared sites of replication initiation and termination in untransformed

88 investigated the role of YabA in regulating replication initiation and the activity of DnaA as a tra

89 ole of protein kinase CK2 in controlling DNA replication initiation and the expression levels of repl

90 We demonstrate that SLD-2 is required for replication initiation and the nuclear retention of a cr

91 activate CtrA-dependent genes following DNA replication initiation and the reaccumulation of CtrA.

92 se checkpoint in mammalian cells to suppress replication initiation and to protect replication forks.

93 dent pathway mediating the inhibition of DNA replication initiation, and a SpoT-independent pathway(s

94 r fundamental understanding of bacterial DNA replication initiation, and because of the conserved str

95 that confers stable episome maintenance, DNA replication initiation, and chromatin organization funct

96 ssion can support organized, yet stochastic, replication initiation, and its absence could explain in

97 f pre-replication complex (pre-RC) assembly, replication initiation, and micrococcal nuclease (MNase)

98 K is an S-phase-specific kinase required for replication initiation, and the DNA primase-polymerase i

99 We also found that DnaN activates replication initiation apparently through effects on Yab

100 r, these results show that cell division and replication initiation are independently controlled at t

101 Perturbation of DNA replication initiation arrests human cells in G1, pointi

102 By picturing replication initiation as a two-state system and conside

103 tion of SirA enforces diploidy by inhibiting replication initiation as B. subtilis cells develop into

104 r mechanism(s) of action at a distance, i.e. replication initiation at a considerable distance from t

105 h a model, suggesting that RepID facilitates replication initiation at a distinct group of human repl

106 , RecQ4, Treslin, and Drf1--are limiting for replication initiation at increasing N/C ratios in vitro

107 Previous work in Leishmania detected DNA replication initiation at just a single region in each c

108 nt gram positive bacteria and is involved in replication initiation at oriC and re-start of collapsed

109 onsistent with models in which YabA inhibits replication initiation at oriC, and perhaps DnaA functio

110 ound that YabA function is mainly limited to replication initiation at oriC.

111 nations of chromosomal orc genes showed that replication initiation at oriC1 requires orc7 but not or

112 bility by providing a checkpoint that delays replication initiation at OriP.

113 icase loading, and is a pivotal event during replication initiation at replication origins.

114 iates diagnostic of canonical bi-directional replication initiation at the centrally located bi-direc

115 r by viral protein-driven multiple rounds of replication initiation at viral origins.

116 itutions of the 3 residues are functional in replication initiation but are immune to inhibition by S

117 ria regulate the frequency and timing of DNA replication initiation by controlling the activity of th

118 Soj is a dynamic protein that regulates DNA replication initiation by either inhibiting or activatin

119 Bacteria control the timing of replication initiation by regulating the activity of Dna

120 This suggests that defects in DNA replication initiation can lead to activation of the DNA

121 By clearly defining the DNA replication initiation checkpoint in hypoxic cells, we n

122 n is dependent on polar remodeling and a DNA replication initiation checkpoint that is tightly integr

123 gin domain plays a role in the regulation of replication initiation, chromosome organization, and DNA

124 een E1 and E2, preventing pre-initiation and replication initiation complex formation.

125 promotes DNA unwinding and the assembly of a replication initiation complex.

126 loss of OBP function or OBP itself from the replication initiation complex.

127 prereplication complexes but were present in replication initiation complexes on ori-Lyt.

128 were observed for 11 and 15 on corresponding replication initiation complexes.

129 of transcription and the enhancement of DNA replication initiation, contains two GAGGC sequences arr

130 l oligomer in vivo and that the frequency of replication initiation correlates with the extent of Dna

131 ependent phenotype as the rid phenotype (for replication initiation defective).

132 Here we identify a replication-initiation determinant (RepID) protein that

133 Displaced Mcm2-7 double hexamers support DNA replication initiation distal to the loading site in vit

134 al states in response to both nucleotide and replication initiation/elongation factors.

135 clease activity, DNA synthesis is slowed and replication initiation events are more frequent.

136 ifferent cell types and host ~80% of all DNA replication initiation events in any cell population.

137 veal that the role of limiting the number of replication initiation events is to prevent DNA topologi

138 its associated E3s are rescued by increasing replication initiation events through mutating two maste

139 hosphorylation modulates the distribution of replication initiation events to insure genomic stabilit

140 of replication initiation sites showed that replication initiation events were absent from transcrip

141 s with high rates of transcription, very few replication initiation events were detected.

142 Moreover, very few replication initiation events were observed when two ect

143 me-wide massively parallel sequencing to map replication initiation events, thereby creating a databa

144 sequences strongly affected the location of replication initiation events, whereas histone modificat

145 progression while reducing the frequency of replication initiation events.

146 ine the spatial and temporal organization of replication initiation events.

147 ally required throughout the S-phase for all replication initiation events.

148 ather acts as a tunable rheostat to regulate replication initiation events.

149 nes the timing of both the next division and replication initiation events.

150 pends on the spatial and temporal pattern of replication initiation events.

151 ormal replication fork progression and fewer replication-initiation events.

152 expressed proteins identified, Cdc6 is a DNA replication initiation factor and exhibits oncogenic act

153 uggest that WDHD1 may also function as a DNA replication initiation factor as well as a G1 checkpoint

154 osis in fission yeast has identified the DNA replication initiation factor cdc18 (known as CDC6 in ot

155 phogenetic protein (BMP) pathway and the DNA replication initiation factor Cdc6.

156 es the DNA damage-induced degradation of the replication initiation factor Cdt1 via the CRL4(Cdt2) E3

157 Interestingly, the DNA replication initiation factor Cdt1, whose uncontrolled e

158 Geminin is known to inhibit the replication initiation factor Cdt1.

159 ecognition complex (ORC) is an essential DNA replication initiation factor conserved in all eukaryote

160 eplication by targeting the highly conserved replication initiation factor DnaA.

161 ta-TRCP)-dependent degradation of a limiting replication initiation factor Drf1.

162 The eukaryotic DNA replication initiation factor Mcm10 is essential for bot

163 We identified a mutation of the replication initiation factor Sld3, Sld3-m16, that is sp

164 And-1/Ctf4 is therefore a new replication initiation factor that brings together the M

165 ation of cell division cycle 6 (Cdc6), a DNA replication initiation factor, by polo-like kinase 1 (Pl

166 ted by a region homologous to the yeast Sld2 replication initiation factor, followed by a cysteine-ri

167 es, replication protein A encoding gene, DNA replication initiation factor, securin gene, nucleosome

168 inus of RECQ4 shares homology with yeast DNA replication initiation factor, Sld2, and is vital for em

169 ingly, pX increases expression of endogenous replication initiation factors Cdc6 and Cdtl while suppr

170 n fires is related to its ability to recruit replication initiation factors that are limiting within

171 on is a member of an expanding subset of DNA replication initiation factors that are not strictly req

172 teases regulate DNA replication by degrading replication initiation factors, suggesting a model in wh

173 By inhibiting replication initiation factors, we show that Cyclin E-in

174 of genetic material depends on essential DNA replication initiation factors.

175 The ability of Cdks to differentially effect replication initiation, factory activation, and progress

176 Bacillus subtilis DnaD is needed during replication initiation for assembly of the replicative h

177 LE may play a role in recombination-mediated replication initiation found in the mitochondria of mamm

178 Despite an increase in replication initiation frequency, cells lacking Mus81 us

179 e levels were generally associated with high replication initiation frequency.

180 and its kinase activity is required for DNA replication initiation from each origin.

181 Under such conditions, uncoupling of replication initiation from mitotic progression led to a

182 is essential for deoxyribonucleic acid (DNA) replication initiation from yeast to humans.

183 but rapidly adapted through mutations in the replication initiation gene trfA1.

184 A role in DNA replication initiation has been demonstrated and mapped

185 ly been shown to regulate DnaA-dependent DNA replication initiation; however, the mechanism underlyin

186 ctionally address their requirements for DNA replication initiation in a series of independent approa

187 yabA encodes a negative regulator of replication initiation in Bacillus subtilis and homologu

188 YabA, a negative regulator of replication initiation in Bacillus subtilis, interacts w

189 Our results shed light on why replication initiation in bacteria is limited to a singl

190 regulatory mechanism for the control of DNA replication initiation in bacteria.

191 nding of the molecular details that underpin replication initiation in bacteria.

192 SeqA protein negatively regulates replication initiation in Escherichia coli and is also p

193 ew insight into the mechanisms that regulate replication initiation in Escherichia coli.

194 rved uncertainty on the position and time of replication initiation in eukaryote cell populations.

195 se and show that MCM2 deficiency reduces DNA replication initiation in gene-rich regions of the genom

196 that the optimal cell cycle progression and replication initiation in M. tuberculosis requires that

197 The genetic aspects of oriC replication initiation in Mycobacterium tuberculosis are

198 MRN at origin-proximal sites to control DNA replication initiation in response to DNA damage, thereb

199 and regulation are diverged features of DNA replication initiation in T. brucei, providing new insig

200 , indicating that ATR kinase activity limits replication initiation in the absence of damage.

201 TDs to bind multiple partners to orchestrate replication initiation in the bacterial cell.

202 basis for further mechanistic dissection of replication initiation in vertebrates.

203 hromatin-binding studies show that E4 blocks replication initiation in vitro by preventing loading of

204 the set of yeast core factors sufficient for replication initiation in vitro is conserved in metazoa.

205 portant for downregulating CtrA prior to DNA replication initiation in vivo but that other phosphatas

206 During replication initiation in vivo, it is not known if these

207 Failure to inhibit replication initiation indeed causes increased DNA caten

208 Replication initiation is a key event in the cell cycle

209 an understanding of the complexities of how replication initiation is controlled and coordinated wit

210 and an origin-proximal promoter involved in replication initiation is delayed.

211 Control of DNA replication initiation is essential for bacterial cells

212 Control of DNA replication initiation is essential for cell growth.

213 Control of DNA replication initiation is essential for normal cell grow

214 ear how this association is terminated after replication initiation is finished.

215 ols the MBT and shows that the regulation of replication initiation is fundamental for normal embryog

216 g metazoan development and proposes that DNA replication initiation is governed differently in mitoti

217 Eukaryotic replication initiation is highly regulated and dynamic.

218 In summary, these findings indicate that replication initiation is influenced by transcription in

219 During replication stress, replication initiation is stimulated downstream of genes

220 th variability, invalidating the notion that replication initiation is the principal trigger for cell

221 me being circular, reinforcing the idea that replication initiation is triggered repeatedly by fork c

222 How Mcm10 is localized to sites of replication initiation is unclear, as current models ind

223 Zones of replication initiation (IZs) were detected throughout S

224 kinase and CDK targets at origins regulates replication initiation kinetics during S-phase and estab

225 Our data suggest that increased replication initiation leads to slow replication fork pr

226 We purified components of the replication initiation machinery and studied their inter

227 Here, we report that the replication initiation machinery of D. melanogaster unex

228 modelling have yielded detailed genome-wide replication initiation maps.

229 Replication initiation must be a carefully regulated pro

230 ate that elevated levels of helicase perturb replication initiation not only at origins of replicatio

231 of Hda is tightly controlled to ensure that replication initiation occurs only once per cell cycle.

232 lays a key role during the transcription and replication initiation of vRNA genome.

233 but these were not directly associated with replication initiation or ongoing chromosome replication

234 ular processes of transcription, DNA repair, replication initiation, or polymerase elongation and the

235 m DNA molecules, as well as in bidirectional replication initiation outside of the main replication o

236 Replication initiation peaks were AT rich, and >50% of t

237 riments, and we discovered that cell size at replication initiation per origin, namely the initiation

238 initiation sites at oriC1 were determined by replication initiation point analysis and found to map d

239 plication programme employs subtelomeric DNA replication initiation, possibly extending beyond S phas

240 gin involves interactions of the RK2 plasmid replication initiation protein (TrfA) with both the poly

241 the ubiquitously conserved bacterial master replication initiation protein DnaA at the oriC but stru

242 a a transcriptional response mediated by the replication initiation protein DnaA.

243 Here we show that the replication initiation protein Sld3 is phosphorylated by

244 the ability of Cdk2 to phosphorylate the key replication initiation protein treslin in vitro Furtherm

245 Overall, this study reveals that TbMCM-BP, a replication initiation protein, also guides the initiati

246 biting expression or function of the plasmid replication initiation protein, RepE.

247 hough they do not resemble any characterized replication initiation protein.

248 Nicking is performed by a replication-initiation protein (Rep) that directly binds

249 lication caused by overexpression of various replication initiation proteins such as Dpb11 and Sld2.

250 ies of hypomorphic alleles of genes encoding replication initiation proteins suggest the question of

251 Replication initiation proteins, known as prereplicative

252 t-range IncP-1 plasmids generally encode two replication initiation proteins, TrfA1 and TrfA2.

253 DnaD and DnaB are essential DNA-replication-initiation proteins in low-G+C content Gram-

254 Lowering the DNA replication initiation rate by introducing the dnaA(Sx)

255 tion and the beta-globin Replicator, the DNA replication-Initiation Region from the beta-globin locus

256 which corresponds with previously mapped DNA replication initiation regions and is demarcated by loca

257 Eukaryotic DNA replication initiation relies on the origin recognition

258 six essential ATPases whose contributions to replication initiation remain unclear.

259 Plasmid encoded replication initiation (Rep) proteins recruit host helic

260 into three measurable variables representing replication initiation, replication-division cycle, and

261 ication elongation, mutants defective in DNA replication initiation require the Chk1 kinase.

262 Template unwinding during DNA replication initiation requires the loading of the MCM h

263 or PPP2R2A deficiency-induced alterations of replication initiation/RS and sensitivity to ATR/CHK1 in

264 mplexes in a manner that exposes a potential replication initiation site.

265 ries of restriction fragments that contained replication initiation sites (bubbles) in vivo.

266 nct DNA-protein interactions at subgroups of replication initiation sites (replication origins) modul

267 ntiate TNR instability; therefore, we mapped replication initiation sites and prereplication complex

268 s short primers revealed the presence of two replication initiation sites at the template strand and

269 findings indicate that Mcm10 is localized to replication initiation sites by directly binding MCM thr

270 by HU suggests that there are many potential replication initiation sites in permissive parts of the

271 Mapping of replication initiation sites in the HAC revealed that re

272 High-resolution mapping of replication initiation sites showed that replication ini

273 Data presented here identify replication initiation sites throughout the genome, prov

274 n events, thereby creating a database of all replication initiation sites within nonrepetitive DNA in

275 Orc2 and Mcm4 bound near each of the replication initiation sites, but a dramatic change in (

276 s, resulting in a corresponding shift in DNA replication initiation sites.

277 define a new mechanism for targeting ORC to replication initiation sites.

278 inant (RepID) protein that binds a subset of replication-initiation sites.

279 These data suggest a conserved mechanism for replication initiation: Sld3/Treslin coordinates Cdc45 r

280 rapid cell lethality, disrupts the timing of replication initiation, slows replication elongation, an

281 e of the 3'-CCUGU motif completely abolished replication initiation, suggesting a crucial role of thi

282 ry - the factors that demarcate sites of the replication initiation, termed origins - are diverged fr

283 essential for replisome assembly during DNA replication initiation that is vulnerable to inhibition

284 This is due to the absence of replication initiation that would typically occur upstre

285 During replication initiation, the core component of the helica

286 Shortly after replication initiation, the duplicated origins move as a

287 cation resumed from oriC after inhibition of replication initiation, these high levels of binding dec

288 sents a repressive compartment that inhibits replication initiation, thus maintaining the boundaries

289 nctionally distinct Rpd3 complexes to affect replication initiation timing supports the idea that his

290 e-cell growth rates, cell division sizes and replication initiation volumes.

291 n gene expression, separate from its role in replication initiation, we analyzed changes in mRNA leve

292 vitro assay for eukaryotic origin-dependent replication initiation, we investigated the control of t

293 t explain how monomers outcompete dimers for replication initiation when plasmid copy number and pi s

294 ity could also provide the physical basis of replication initiation where a double-hexamer idly encir

295 propose a model for Sld3-mediated control of replication initiation, wherein Sld3 manages the proper

296 kinase-independent function for AURKA in DNA replication initiation whose inhibition through a class

297 ribe the reconstitution of budding yeast DNA replication initiation with 16 purified replication fact

298 ee extracts we established a system to study replication initiation within linear telomeric DNA subst

299 upport predominantly chromosome-internal DNA replication initiation within S phase.

300 In S-phase, the checkpoint inhibits replication initiation, yet the function of this global