ライフサイエンスコーパス: replicon

1 ded for RNA genome replication (intragenomic replicons).

2 y decreases replication of an HEV subgenomic replicon.

3 ory effect of cGAMP than was that of 1b/Con1 replicon.

4 ectious virus and a corresponding subgenomic replicon.

5 mic acids for inhibition of a HCV subgenomic replicon.

6 n sRNA and the name of a sequenced bacterial replicon.

7 d contains a stably replicating HCV reporter replicon.

8 lation-deficient, replication-defective JFH1 replicon.

9 ts too large to be accounted for by a single replicon.

10 n for daclatasvir compared to the unmodified replicon.

11 P) reporter-expressing West Nile virus (WNV) replicon.

12 from cells that harbor an HCV subgenomic RNA replicon.

13 s-complementation of subgenomic reporter HEV replicons.

14 lex entities that process multiple clustered replicons.

15 ded among two or more large chromosome-sized replicons.

16 chromatin context at the level of individual replicons.

17 -associated translation activity in the RNA2 replicons.

18 tures or after electroporation of selectable replicons.

19 uelan equine encephalitis virus (VEEV)-based replicons.

20 says using western equine encephalitis virus replicons.

21 s as a mechanism for the origin of novel RNA replicons.

22 icromolar potency against both G-1a and G-1b replicons.

23 pic screening campaigns using HCV subgenomic replicons.

24 rational means to inhibit these tumorigenic replicons.

25 linical polymorphic and resistant HCV mutant replicons.

26 tain stable, genotype 4a luciferase-encoding replicons.

27 ular level utilizing hepatitis C virus (HCV) replicons.

28 cies and broad-spectrum activity against HCV replicons.

29 ies have been achieved using DNA virus-based replicons.

30 Cas9 when they are delivered on geminivirus replicons.

31 anisms similar to those found in other ssDNA replicons.

32 by using genotype 4 NS5A hybrid genotype 2a replicons.

33 in antigens are encoded by encapsulated mRNA replicons.

34 ith a focus on the role of each of its three replicons.

35 Using chimeric replicons, 2a NS4B was identified to confer resistance t

36 ness in the soil after deletion of its third replicon, a non-essential plasmid associated with virule

37 rld include those expected for primitive RNA replicons: (a) small size imposed by error-prone replica

38 In wheat cells, the replicons achieve a 110-fold increase in expression of a

39 Although the replicon activity of the new agents containing pyrimidin

40 d following inter-species mating target self-replicons along with those originating from the mating p

41 o be >99% based on results from in vitro HCV replicon amplification, with the same extrapolated amoun

42 rom cells harboring a replicating subgenomic replicon and analyzed the purified protein by mass spect

43 Both the WEEV replicon and combination V/W/E vaccination, however, eli

44 ay analysis of flies harboring an alphavirus replicon and control green fluorescent protein flies ide

45 double-stranded RNA (dsRNA) or an FHV-based replicon and facilitates the natural infection of C. ele

46 ectedly, replication of the genotype 2a JFH1 replicon and infectious JFH1 virus was less sensitive to

47 licon but was less effective for the 2a/JFH1 replicon and infectious JFH1 virus.

48 ntent, and existing classifications based on replicon and mobility (MOB) types.

49 l of CHIKV infection and showed that the DNA replicon and protein antigen were potent vaccine candida

50 ibe the development and testing of novel DNA replicon and protein CHIKV vaccine candidates and evalua

51 Using a PV replicon and purified virion RNA, we also show that eIF4

52 We tested Motif V mutations in both the replicon and recombinant protein systems to investigate

53 , finds fragments of those tDNAs in the same replicon and removes unlikely candidate islands through

54 zed and transcribed a genotype 4a subgenomic replicon and transfected Huh7-Lunet cells with it, which

55 Similarly, the EEEV replicon and V/W/E combination vaccine elicited neutrali

56 ication of infectious SARS-CoV as well as of replicons and human coronavirus NL63.

57 TNF had little effect on HCV replicons and increased entry of HCV pseudoparticles.

58 ctivities using genotype 2a JFH-1 subgenomic replicons and infectious virus systems.

59 vitro potency against wild-type hepatitis C replicons and known clinical polymorphic and resistant H

60 tic insertion of this tag resulted in viable replicons and specific labelling while preserving the ef

61 y leading to the establishment of subgenomic replicons and the infectious virus model (HCVcc).

62 456 inhibits an artificial Hepatitis C viral replicon, and has broad antifungal activity, suggesting

63 n be formed with multiple antigen-expressing replicons, and is capable of eliciting both CD8(+) T-cel

64 However, it remains unknown how replicons are organized for processing at each replicati

65 havirus genome-based, self-replicating RNAs (replicons) are widely used vectors for expression of het

66 ngs, effective replication of subgenomic HCV replicons as well as production of infectious virus part

67 in 10-fold improvement in potency in a WEEV replicon assay and up to 40-fold increases in half-lives

68 bited the most promising activity in the RSV replicon assay with an EC50 of 0.15 muM.

69 ve in the hepatitis C virus (HCV) cell-based replicon assay, which was corroborated on examination of

70 -5172 activity was determined using a mutant replicon assay.

71 ovis and also against hepatitis C virus in a replicon assay.

72 ral effects against hepatitis C virus in the replicon assay.

73 Reporter virus and replicon assays using phosphorylation-ablated alanine mu

74 type that established a potency benchmark in replicon assays, particularly toward HCV GT-1a, a strain

75 Using Northern blot and reporter replicon assays, we demonstrated that both small molecul

76 pounds showing submicromolar activity in HCV replicon assays.

77 revir and daclatasvir was assessed using HCV replicon assays.

78 compared with a reference strain in in vitro replicon assays.

79 echanism by which RepA mediates the specific replicon assembly of staphylococcal multiresistant plasm

80 In tobacco (Nicotiana tabacum), replicons based on the bean yellow dwarf virus enhanced

81 ncy against the NS3 protease in a subgenomic replicon-based cellular assay (Huh-7).

82 stress generated during HCV replication in a replicon-based model also induced STAT3 activation.

83 In the present work, we developed a replicon-based system for genome engineering of cereal c

84 inhibitor) patients and 344 genotype 1 to 6 replicons bearing engineered NS3 resistance-associated s

85 e mathematical model showing how neighboring replicons became associated stochastically to form repli

86 We recently reported on intragenomic replicons, bicistronic viral transcripts expressing an a

87 ibit the replication of HCV genotype 1b Con1 replicon but was less effective for the 2a/JFH1 replicon

88 priming sites of the DNA primase of the pRN1 replicon, but nearly all these mutations created nonsens

89 uced into either ORF within the intragenomic replicon, but unlike many other mutations required the o

90 sion of double-stranded DNA from an episomal replicon by CRISPR/Cas9, coupled to lambda-red-mediated

91 Furthermore, replicons carrying CRISPR/Cas9 nucleases and repair temp

92 The three largest replicons (CC, LC, pAt) reside in the OP compartment pri

93 antitatively measured using HCV infected and replicon cell culture.

94 viral activities against CHIKV using a CHIKV replicon cell line and clinical isolate of CHIKV of Cent

95 ng from 0.2 to >98 muM, measured in the Huh7-replicon cell line, with no apparent cytotoxicity (CC50

96 expression in HCV-infected hepatocytes using replicon cells containing full-length HCV genotype 1 and

97 ed that the autophagosomes purified from HCV replicon cells could mediate HCV RNA replication in a li

98 ith genotype 1a full-length (FL) HCV genomic replicon cells or genotype 2a Japanese fulminant hepatit

99 The addition of cGAMP into 1b/Con1 replicon cells significantly increased the expression of

100 amma when PBMC were cocultured with Huh7/HCV replicon cells than with Huh7 cells; NK cells and PBMCs

101 Replicon cells were labeled with virus-specific peptides

102 se-tagged subgenomic HCV replicons (Huh7/HCV replicon cells) or their HCV-negative counterparts (Huh7

103 mpounds showing (i) nanomolar potency in HCV replicon cells, (ii) limited toxicity and off-target act

104 icacy in a triple combination regimen in HCV replicon cells, and exhibited consistently high oral bio

105 ional proteomic screening carried out in HCV replicon cells, we identified the mitochondrial protein

106 tween IRF5- and empty vector-transfected HCV replicon cells.

107 Agrobacterium tumefaciens C58 contains four replicons, circular chromosome (CC), linear chromosome (

108 abundance of resistance genes and of plasmid replicons, coinciding with a decline in the number of in

109 adaptive mutations, and selected for stable replicon colonies.

110 e cellular diversity of subgenomic JFH-1 HCV replicons constitutively expressed in Huh7 cells.

111 5A alone from an additional cistron within a replicon construct gave greater rescue of virion assembl

112 Furthermore, replication of a HEV replicon containing green fluorescent protein (GFP) (E2-

113 d gt1b NS5A variants as well as HCV chimeric replicons containing NS5A fragments from genotypes 2-6.

114 patterns of luciferase expression from virus replicons containing the Gaussia luciferase gene in plac

115 Replicons containing the most effective mutation combina

116 sponse and created modified forms of HCV and replicons containing the substitutions of interest and t

117 ation fitness and sensitivity of various HCV replicons, containing or lacking NS2, to cyclosporine an

118 ily support the complete RHV life cycle, but replicon-containing cell clones could be selected with a

119 In the Huh-7 HCV genotype (GT) 1b replicon-containing cell line 9 is devoid of any anti-HC

120 construction of an improved broad-host-range replicon derived from RSF1010, which replicates in sever

121 mined by immunoblotting, proximity ligation, replicon DNA replication and whole virus immunofluoresce

122 regulated at the level of large-scale multi-replicon domains.

123 We analyze how individual replicons dynamically organized a replication factory us

124 Compound 39 (GT1a replicon EC50 = 31 pM) has an extended plasma half-life

125 antiviral potency in genotype (gt) 1a and 1b replicons (EC50 = 120 and 110 pM, respectively) and with

126 he VSV glycoprotein gene is expressed from a replicon encoding the nonstructural proteins of Semliki

127 A modified replicon encoding West Nile virus (WNV) premembrane and

128 sed on engineered self-amplifying mRNA (SAM) replicons encoding an Ag, and formulated with a syntheti

129 kaging cell line can be transfected with HCV replicons encoding cognate Jc1-derived glycoprotein gene

130 ene deletion mutants suggests that secondary replicons evolved to fulfil a specialized function, part

131 (VLVs) are generated when an alphavirus RNA replicon expresses the vesicular stomatitis virus glycop

132 Testing replicons expressing representative envelope protein gen

133 Subgenomic RV-A and RV-C RNA replicons failed to amplify in the absence of STING, rev

134 range of geminiviruses, advocate the use of replicons for plant genome engineering.

135 Here, we use geminivirus-based replicons for transient expression of sequence-specific

136 e impact of SIL on replication of subgenomic replicons from different HCV genotypes in vitro and foun

137 trans-complementing genomes (referred to as 'replicons') from the arbovirus Sindbis [2].

138 which different types of primordial, selfish replicons gave rise to viruses by recruiting host protei

139 us to uncover candidates for yet undescribed replicon genes, and to identify transposable elements as

140 -stereochemistry (EC50 = 3.4 +/- 0.2 pM, HCV replicon genotype 1b), was dramatically more active than

141 that sCD55 is induced in HCV-infected or HCV replicon-harboring cells, as well as in liver biopsy sam

142 DNA replication 50 years ago, the predicted replicons have not been identified and quantified at the

143 XP-knockout replicons have only a minor replication defect, demonstr

144 localization of proteinaceous complexes and replicons; hence they are instrumental for myriad cellul

145 sase genes and AMR genes, as well as plasmid replicons, highlights the potential role of horizontally

146 hat express luciferase-tagged subgenomic HCV replicons (Huh7/HCV replicon cells) or their HCV-negativ

147 Using an FMDV replicon in complementation experiments, our data demons

148 analyzed replication of the subgenomic JFH1 replicon in embryonic fibroblasts and primary hepatocyte

149 rus (HCV) genotype 1b strain Con1 subgenomic replicon in human hepatoma cells.

150 ary trajectories as its non-conjugative mini-replicon in the same and other species.

151 Here we examine 2 functionally distinct replicons in the same cells to dissect DNA amplification

152 cells, as well as transfection of luciferase replicons in two types of cardiomyocytes, we demonstrate

153 Using luciferase constructs/replicons, in vivo and in vitro assays showed that the 5

154 f new VLoop variants that made CHIKV and its replicons incapable of inhibiting cellular transcription

155 Using sub-genomic replicons incorporating nonfunctional 3Bs and 3B fusion

156 accumulation in a cell line harboring a ZIKV replicon, indicating that EMC participates in the comple

157 Analyses were conducted with replicons, infectious virus, and human hepatoma cells th

158 CV genotype 1a (G-1a) and genotype 1b (G-1b) replicon inhibition and selectivity against BVDV and cyt

159 gm for regulating the sites where individual replicons initiate replication.

160 The genomic architecture of the eccDNA replicon is composed of a complex arrangement of repeat

161 The fundamental unit of DNA replication, the replicon, is governed by a cis-acting replicator sequenc

162 y, transfected the cells with HCV subgenomic replicons lacking adaptive mutations, and selected for s

163 Even with subgenomic replicons, lacking structural viral proteins, exosome-me

164 ered in E1A-expressing cells, with increased replicon length, fork velocity, and interorigin distance

165 Replicon levels and drug-resistant variants were quantif

166 Use of a replicon library with codon degeneracy did allow identif

167 Thus, these mutant replicons may be useful for improving RNA therapeutics f

168 Thus, genes on secondary replicons might potentially be manipulated to promote or

169 Since the pioneering proposal of the replicon model of DNA replication 50 years ago, the pred

170 Concurrent with the replicon model was Taylor's demonstration that plant and

171 on mechanism that departs from the classical replicon model, helping eukaryotic cells to negotiate tr

172 l principles and concepts established in the replicon model.

173 itional layers of regulation to the original replicon model.

174 buvir was measured using the S52 DeltaN gt3a replicon model.

175 iral proteins, combined with high-throughput replicon models, enabled the discovery and development o

176 wheat genome, and we show that with the WDV replicons, multiplexed GT within the same wheat cell can

177 uation of both CpG- and UpA-high viruses and replicon mutants was reversed in ZAP k/o cell lines, and

178 In comparison to wildtype replicon, mutants expressing IL-2 injected into murine B

179 However, the mutant form of a subgenomic replicon of genotype 3 HEV replicated more efficiently i

180 setting of cellular division, when two viral replicons of equivalent fitness are present within a cel

181 NS2-5B replicons of genotype 2a (JFH1), but not genotype 1b, ha

182 -derived cell lines with subgenomic reporter replicons of HAV as well as of different HCV genotypes,

183 ication, which was observed using subgenomic replicons of two different genotypes.

184 They also show that the intragenomic replicon offers a unique way to study replication comple

185 lication were observed for specific LCV RNA2 replicons only in the presence of LCV RNA1, but both pro

186 n cells expressing a full-length genotype 1b replicon or infected with the JFH-1 strain of HCV.

187 harboring subgenomic hepatitis C virus (HCV) replicons or infected with cell culture-derived HCV were

188 levels of IFN-alpha after coculture with FL replicons or JFH-1-infected cells.

189 ce was found of persistent extra-chromosomal replicons or off-target integration of T-DNA or replicon

190 ction were assessed in HCV-permissive cells, replicons, or human embryonic kidney (HEK) 293 cells tra

191 While some behaved similarly to the S232I replicon, others displayed a unique trans-complementatio

192 by self-amplifying mRNA packaged in a viral replicon particle (VRP) or by a recombinant HIV envelope

193 (SUDV)- and Ebola virus (EBOV)-specific VEEV replicon particle (VRP) vaccines in nonhuman primates.

194 ressing Venezuelan equine encephalitis viral replicon particle vaccine protected these mice from SUDV

195 Kp47/47-Venezuelan equine encephalitis virus replicon particle) for safety, immunogenicity, and effic

196 ased on Venezuelan equine encephalitis virus replicon particles (VRP) expressing two configurations o

197 outs, but was largely inferior to virus-like replicon particles (VRP) or direct electroporation.

198 cine candidate composed of single-cycle LASV replicon particles (VRPs) and a stable cell line for the

199 zuelan equine encephalitis virus (VEE) empty replicon particles (VRPs) can induce rapid protection of

200 tial vaccine [Venezuelan equine encephalitis replicon particles expressing MERS-CoV spike protein].

201 e nine alanine mutants affected the entry of replicon particles, which correlated with the impairment

202 production of both virus-like particles and replicon particles.

203 virus particles upon transfection with a GFP replicon plasmid.

204 mbination therapy was additive for the total replicon population and the LDV-resistant population, bu

205 Cell-based replicon potency was significantly improved through elec

206 Transpositions were also detected, revealing replicon preference (ISKpn18 prefers a conjugative IncA/

207 These replicons produce SG RNAs and encoded proteins of intere

208 Removal of the third replicon reduced B. ambifaria persistence in a murine re

209 pression diminished the inhibitory effect on replicon replication, while overexpression of STING enha

210 lation between hyperphosphorylation and JFH1 replicon replication.

211 located on a 141-kb plasmid with multiple F replicons (replicon type: F36:A4:B1).

212 es of 50 and 9 pM toward genotype-1a and -1b replicons, respectively, and oral bioavailabilities of 3

213 /L320F into genotype 1a (H77) and 1b (Con-1) replicons, respectively, increased the EC50 for mericita

214 DHCR24*) in cells harboring a HCV subgenomic replicon RNA or ectopically expressing NS3-4A; and bioch

215 ed and indicated an average of 113 copies of replicon RNA per cell, correlating with calculated RNA c

216 sential for accumulation of GBV-B subgenomic replicon RNA.

217 in NS4B and NS5A and in cell clones cured of replicon RNA.

218 Self-replicating (replicon) RNA is a promising new platform for gene thera

219 f JNK1 and JNK2, enhanced replication of HCV replicon RNAs as well as infectious genome-length RNA tr

220 erviral recombinants between two plant virus replicon RNAs were identified in N. benthamiana plants,

221 licons or off-target integration of T-DNA or replicon sequences.

222 quantification of replication fork speed and replicon size in human and mouse cells.

223 Once associated, however, replicons stay together relatively stably to maintain re

224 the genomic origins of the A. palmeri eccDNA replicon structure.

225 say replication from an endogenous transgene replicon, suggesting that DRL-1 affects a prereplication

226 tomato genome was achieved using geminivirus replicons, suggesting that these vectors can overcome th

227 CC50 > 224 muM; SI > 28) in a cell based HCV replicon system assay.

228 ecific CD8 T cells have been shown in an HCV replicon system but not in an authentic infectious HCV c

229 evalence of hepatitis C virus genotype 4, no replicon system is available for study of the genotype.

230 We used a subgenomic replicon system to assess the effects of the same mutati

231 RNA replication from an endogenous transgene replicon system was not affected by lack of HIPR-1, sugg

232 The genotype 4a replicon system we created will aid in the development o

233 ded by HCV NS3 protease assays, the cellular replicon system, structure-based design, and a panel of

234 25.6 muM, and CC50 > 180 muM in the Huh 9-13 replicon system, thus providing a good starting point fo

235 Using the replicon system, we show that the RHV-rn1 NS3-4A proteas

236 Making use of the Con1 HCV replicon system, we tested the effect of EMR proteins on

237 d not confer resistance to sofosbuvir in the replicon system.

238 re further examined in the JFH-1 genotype 2a replicon system; importantly, all mutations that destabi

239 Reliance on hepatitis C virus (HCV) replicon systems and protein-based screening assays has

240 atitis E virus genotype 3 both in subgenomic replicon systems as well as a full-length infectious clo

241 phase of the virus life cycle in vitro These replicon systems enabled identification of replication-e

242 filoviruses but also for the design of other replicon systems widely used as surrogate systems to stu

243 ch, in both infectious virus and sub-genomic replicon systems, we identified six RNA replication elem

244 RNA in both infectious virus and subgenomic replicon systems.

245 rally elicited a higher PCN of an IncP1-beta replicon than strains expressing TrfA2 alone.

246 We engineered an infectious TVCV replicon that expressed a functional fluorescence-tagged

247 ingle, synthetic self-replicating VEE-RF RNA replicon that expresses four reprogramming factors (OCT4

248 by deletion of the VSIV G gene to generate a replicon that is dependent on trans expression of G prot

249 sPs) of Venezuelan equine encephalitis (VEE) replicon that promoted subgenome expression in cells.

250 on of the 10 kGy IR-shattered D. radiodurans replicons that correlates with the timing of DraRnl repl

251 tform consisting of Semliki Forest virus RNA replicons that express the vesicular stomatitis virus gl

252 The "Replicon Theory" of Jacob, Brenner, and Cuzin has reliab

253 lls can be optically resolved down to single replicons throughout S-phase.

254 ) and sofosbuvir (SOF) against a genotype 1b replicon to determine optimal exposures for each agent t

255 d or completely abolished the ability of the replicon to replicate, further supporting the concept th

256 The contribution of each replicon to the microbial life cycle (for example, envir

257 Here, we use geminivirus replicons to create heritable modifications to the tomat

258 nstrate the feasibility of using geminivirus replicons to generate plants with a desired DNA sequence

259 These replicons transiently amplify to high copy numbers in pl

260 elegans based on the suppression of a viral replicon-triggered RDVI by ectopic expression of candida

261 a 141-kb plasmid with multiple F replicons (replicon type: F36:A4:B1).

262 genome); substantial plasmid diversity (>/=9 replicon types); and substantial bla KPC-associated, mob

263 gly, pVAPN is a 120-kb invertron-like linear replicon unrelated to the circular virulence plasmids as

264 Previously, a replicon vaccine based on Venezuelan equine encephalitis

265 l replicon vaccines or the combination V/W/E replicon vaccine elicited strong neutralizing antibodies

266 l replicon vaccines or the combination V/W/E replicon vaccine elicited strong neutralizing antibodies

267 ntramuscular, Venezuelan equine encephalitis replicon vaccine expressing EBOV GP.

268 nd humoral immune response than the systemic replicon vaccine.

269 Individual replicon vaccines or the combination V/W/E replicon vacc

270 Individual replicon vaccines or the combination V/W/E replicon vacc

271 These data demonstrate that replicon vaccines successfully bridge the gap between sa

272 , we have extended the analysis with plasmid replicons, virulence factors, and highly discriminatory

273 nce typing, phylotyping, ESBL genes, plasmid replicons, virulence genes, amplified fragment length po

274 of an EBOV vaccine candidate based on Kunjin replicon virus-like particles (KUN VLPs) encoding EBOV g

275 plasmid DNA followed by boosting with Kunjin replicon virus-like particles both encoding a modified E

276 ics and Venezuelan equine encephalitis virus replicons (VRPs) expressing spike and nucleocapsid prote

277 cation, but replication of a minimalized PLE replicon was not sufficient for ICP1 DNA replication int

278 ion of CpG-high, and UpA-high E7 viruses and replicons was also achieved through knockout of RNAseL a

279 Here, using the plasmid RK2 replicon, we analyze the protein interactions required f

280 Using a model vector based on the pINV replicon, we observe temperature-dependent differences b

281 utagenesis reporters spanning a well-defined replicon, we show that both exonuclease-deficient Pole (

282 However, in contrast with Con1 replicons, we observed a positive correlation between hy

283 Alphavirus replicons were evaluated as potential vaccine candidates

284 , antimicrobial resistance genes and plasmid replicons were identified from the genome sequences.

285 HAV and HCV replicons were similarly sensitive to interferon (IFN),

286 The 53BP1 is preferentially bound to larger replicons, where the probability of DFSs is higher.

287 s is necessary for trans replication of FMDV replicons, which is unlike other picornaviruses where a

288 restores HBV replication from a HBx-null HBV replicon, while a shorter peptide, HBx-aa118-127, inhibi

289 sted that the defective NS5A encoded by this replicon, while lacking one NS5A function, was capable o

290 , high frequencies of GT using WDV-based DNA replicons will make it possible to edit complex cereal g

291 An MNV replicon with a frameshift mutation in open reading fram

292 Long-term treatment of subgenomic replicons with 13 potently and durably decreased HCV RNA

293 shed efficient RHV-rn1 selectable subgenomic replicons with and without reporter genes.

294 Luciferase-expressing E7 sub-genomic replicons with CpGs and UpAs removed from the reporter g

295 Linear dsDNA replicons with hairpin ends are found in the three domai

296 ough effects on translation efficiency since replicons with high CpG/UpA sequences inserted into a no

297 Voxilaprevir susceptibilities of HCV replicons with NS3 RASs were dependent on subtype backgr

298 4t has excellent potency against the HCV 1b replicon, with an EC50 = 2 nM and a selectivity index of

299 uted by initiation at clusters of individual replicons within each segment.

300 Surprisingly, we show that the grouping of replicons within factories is highly variable from cell