ライフサイエンスコーパス: replisome

1 vision and is performed by the multi-protein replisome.

2 ion back onto ssDNA to nucleate a functional replisome.

3 rcoil relaxation in front of the progressing replisome.

4 se, and model a single species of "stressed" replisome.

5 r organization and operating principles of a replisome.

6 rases function independently within a single replisome.

7 a conserved protein that helps stabilize the replisome.

8 oenzyme remains stably associated within the replisome.

9 tin-bound cohesin hinders the advance of the replisome.

10 signed to either identical polymerase in the replisome.

11 in the same direction of replication by the replisome.

12 synthesis by the fully reconstituted Pol III replisome.

13 he conserved core of the archaeal/eukaryotic replisome.

14 ncounters a Tus-ter barrier before the other replisome.

15 ntering a Tus-ter roadblock on an individual replisome.

16 advances in the structure of the eukaryotic replisome.

17 t connects multiple accessory factors to the replisome.

18 only capable of binding mismatches near the replisome.

19 uced by mutations that displace Pol from the replisome.

20 , triggered an interest in the dynamics of a replisome.

21 ase upon association of the primase with the replisome.

22 ctivation and function of the eukaryotic DNA replisome.

23 nabled an atomic model of the leading strand replisome.

24 are not required for plasmids containing one replisome.

25 ation of CDC45 and polymerase alpha from the replisome.

26 cA* largely appears at locations distal from replisomes.

27 nt with the disassembly of a fraction of the replisomes.

28 centrally located and may prime both sister replisomes.

29 add to this problem and present barriers to replisomes.

30 e collisions of transcription complexes with replisomes.

31 nitiation and the overall functioning of DNA replisomes.

32 plicative helicase is a crucial component of replisomes.

33 found in the Escherichia coli and eukaryotic replisomes.

34 their interaction with active leading-strand replisomes.

35 Duplication is concerted by replisomes.

36 ntifies a process unprecedented in bacterial replisomes.

37 prevent the chromatin assembly of functional replisomes.

38 ation, we find two different lesion proximal replisomes.

39 ine the resolution pathway of lesion-stalled replisomes.

40 ion elongation complexes block reconstituted replisomes.

41 lity during the resolution of lesion-stalled replisomes.

42 Leading the replisome, a DNA helicase separates the parental strands

43 The origins must be licenced in G1, and the replisome activated at licenced origins in order to gene

44 Although the molecular basis for replisome activity has been extensively investigated, it

45 We propose that the slowdown in replisome activity is a strategy to prevent clashes with

46 se activity, the proofreading subunit of the replisome acts as a gatekeeper and influences replicatio

47 Unexpectedly, the replisome acts as an orientation-dependent regulator of

48 The replisome also coordinates nucleosome disassembly, assem

49 mediate response is localized to the stalled replisome and includes protection of the nascent DNA.

50 4 histone chaperone that associates with the replisome and orchestrates chromatin assembly following

51 DNA lesions stall the replisome and proper resolution of these obstructions is

52 r data provide a quantitative picture of the replisome and replication stress response proteomes in 3

53 directional collisions occurring between the replisome and RNA polymerase.

54 34 proteins needed to form the S. cerevisiae replisome and show how changing local concentrations of

55 elated with its separation distance from the replisome and that MutS motion slows when it enters the

56 ecruitment and retention of Tof1-Csm3 by the replisome and that this complex antagonizes the Rrm3 hel

57 erase gamma is a core component of the mtDNA replisome and the only replicative DNA polymerase locali

58 Conflicts between the replisome and transcription machinery can lead to interr

59 rrest is manifested by a failure to assemble replisomes and by decreased rates of cell growth and rRN

60 system to comprehensively study thermophilic replisomes and evolutionary links between archaeal, euka

61 by checkpoint proteins that protect arrested replisomes and inhibit new initiation when replication i

62 ollisions between DNA replication complexes (replisomes) and barriers such as damaged DNA or tightly

63 vo is created specifically by two convergent replisomes, and demonstrate that the function of RecBCD

64 actor C complex, a critical component of the replisome; and is found at replication forks.

65 ication process, wherein contacts within the replisome are continually broken and reformed.

66 are well understood, its dynamics within the replisome are not.

67 The current view is that eukaryotic replisomes are independent.

68 DNA replication have led to a picture of the replisome as an entity that freely exchanges DNA polymer

69 The eukaryotic replisome assembles around the CMG helicase, which stabl

70 It supports both replisome assembly and leading strand synthesis; however

71 Replisome assembly at eukaryotic replication forks conne

72 ur findings define a mechanism essential for replisome assembly during DNA replication initiation tha

73 Replisome assembly requires the loading of replicative h

74 o the four-member pre-loading complex during replisome assembly.

75 and to melt the DNA helix in preparation for replisome assembly.

76 nt advances in our understanding of TRAIP, a replisome-associated E3 ubiquitin ligase that is mutated

77 We show that replisome-associated factors Mrc1 and Csm3/Tof1 are cruc

78 protein complexes and place newly identified replisome-associated proteins into functional pathways.

79 st and that each requires a different set of replisome-associated proteins.

80 C replication restart factor and observe Rep-replisome association is also dependent on PriC.

81 eading-strand synthesis by the S. cerevisiae replisome at the single-molecule level.

82 of a DNA replication complex (break-induced replisome) at telomeres or elsewhere in the mammalian ge

83 ansmembrane transporters, chromosome loss by replisome binding, and replication stalling by transcrip

84 operate on plasmid substrates containing two replisomes, but are not required for plasmids containing

85 structures, we determine its topology in the replisome by cross-linking mass spectrometry.

86 ze this coordination in the bacteriophage T7 replisome by simultaneously monitoring the kinetics of l

87 Cells use accessory helicases that help the replisome bypass difficult barriers.

88 lymerase III holoenzyme in a stalled E. coli replisome can directly bypass a single cyclobutane pyrim

89 ding the molecular basis for how the E. coli replisome can maintain high processivity and yet possess

90 Replisomes can directly replicate past a lesion by error

91 Alternatively, replisomes can reprime DNA synthesis downstream of the l

92 es, and assembly in vitro and in vivo into a replisome capable of coordinated leading/lagging strand

93 t as a loader protein for the recruitment of replisome cascade proteins.

94 We have investigated replisome collisions with transcription complexes and R-

95 The instability of the replisome complex is conflict-induced: transcription inh

96 Here, we characterize the replisome-complex stoichiometry and dynamics with single

97 We show that the mammalian replisome component C20orf43/RTF2 (homologous to S. pomb

98 at replication protein A (RPA), an essential replisome component that binds single-stranded DNA, has

99 We demonstrate that DONSON is a replisome component that stabilizes forks during genome

100 ing developmental requirements for this core replisome component that warrant future investigation.

101 east replication forks that include all core replisome components and both type I and type II topoiso

102 , our work reveals how four highly conserved replisome components collaborate with CMG to facilitate

103 We conclude that the stochastic behavior of replisome components ensures complete DNA duplication wi

104 Inherited mutations of replisome components have been identified in a range of

105 Recent structures of eukaryotic replisome components include the Mcm2-7 complex, the CMG

106 a multiprotein complex between AURKA and the replisome components MCM7, WDHD1 and POLD1 formed during

107 onjugated to CMG-Mcm7, dependent on multiple replisome components that bind to the ubiquitin ligase S

108 ng by replicative helicases and explains how replisome components that interact with the excluded DNA

109 provide evidence that Rtt107 associates with replisome components, and both itself and its associated

110 axis, but is independent of other canonical replisome components, ATM and ATR, or the homologous rec

111 otential regulators of termination, and many replisome components.

112 Instead, we show that most replisomes continue synthesizing DNA at a slower rate af

113 When two replisomes converge at an interstrand crosslink, TRAIP u

114 Finally, when two replisomes converge they are disassembled.

115 tivation, but how specific components of the replisome coordinate with ATR to activate Chk1 in human

116 emplate damage that acts as obstacles to the replisome, deal with broken forks, and impact human heal

117 , Polzeta-dependent mutagenesis triggered by replisome defects or UV irradiation in vivo was not decr

118 vation occurs in strains, in which intrinsic replisome defects promote the participation of error-pro

119 How the replisome detects and responds to secondary structures i

120 ructural changes within the Escherichia coli replisome determine the resolution pathway of lesion-sta

121 in the rate of synthesis is not explained by replisome disassembly alone.

122 identify CRL2(Lrr1) as a master regulator of replisome disassembly during vertebrate DNA replication

123 show that the TRAIP ubiquitin ligase drives replisome disassembly in response to incomplete DNA repl

124 ve short lifetimes (<8 min), suggesting that replisome disassembly is quite prevalent, possibly occur

125 be rescued in a manner that does not involve replisome disassembly or reassembly, albeit with loss of

126 results identify a mitotic pathway of global replisome disassembly that can trigger replication fork

127 on of DNA synthesis while avoiding premature replisome disassembly.

128 esis, decatenation of daughter molecules and replisome disassembly.

129 Individual replisomes display both looping and pausing during primi

130 nd helicase stay together at the lesion, the replisome does not dissociate and the helicase does not

131 In vitro, purified replisomes drive model replication forks to synthesize c

132 ave revealed the basic principles of how the replisome duplicates genomic DNA, but little is known ab

133 idelity and translesion synthesis within the replisome during DNA replication.

134 * subassembly frequently disengages from the replisome during DNA synthesis and exchanges with free c

135 d DNA polymerases frequently dissociate from replisomes during DNA replication in vivo.

136 AC mass spectrometry, we characterized human replisome dynamics in response to fork stalling.

137 These observations of replisome dynamics provide important insight into the me

138 rrent knowledge of the molecular genetics of replisome dysfunction disorders and discuss recent mecha

139 On the other hand, replisomes easily bypassed R-loops on either template st

140 plexes are one of the principal barriers the replisome encounters during replication.

141 We show that when a replisome encounters the lesion, a substantial fraction

142 that is positioned such that one of the two replisomes encounters a Tus-ter barrier before the other

143 Therefore, we were surprised to find that T7 replisome excised nearly 7% of correctly incorporated nu

144 ase and R-loop clearance mechanisms to limit replisome exposure to these potential obstructions.

145 hesis (TLS)-mediated restart of a eukaryotic replisome following collision with a cyclobutane pyrimid

146 a role of the Pol epsilon catalytic core in replisome formation, a reliance of Pol epsilon strand sy

147 Eukaryotic DNA replication terminates when replisomes from adjacent replication origins converge.

148 ghtly bound protein complexes can dissociate replisomes from chromosomes prematurely.

149 gether, our data suggest that FANCM prevents replisomes from stalling/collapsing at ALT telomeres by

150 on the importance of B-subunit integrity in replisome function in vivo.

151 pancy of multiple DNA polymerases within the replisome has been observed primarily in bacteria and is

152 The T4 replisome has provided a unique opportunity to investiga

153 Nonetheless, components of the replisome have been implicated in human disease, and her

154 In vitro studies of reconstituted E. coli replisomes have attributed this remarkable processivity

155 Whether replisomes have stalled or undergone termination, CMG ub

156 avior and interactions in the context of the replisome, however, remain unclear.

157 e cryoEM structure of human CMG bound to the replisome hub AND-1 (CMGA).

158 sistent with the presence of only one active replisome in a significant fraction of cells (>40%).

159 ht into the organization and dynamics of the replisome in bacterial cells.

160 H3/H4 tetramer suggest a direct role of the replisome in handling nucleosomes, which are important t

161 cal insight into the requirement for the DNA replisome in human NK cell maturation and function.

162 ilizes these complexes, restoring the second replisome in many of the cells.

163 terized the dynamic movement of MutS and the replisome in real time using superresolution microscopy

164 ding is critical for MutS to localize to the replisome in vivo.

165 e Ctf4 partner DNA polymerase alpha from the replisome in yeast extracts.

166 By examining replisomes in live E. coli with fluorescence microscopy,

167 tential role for pol IV in assisting pol III replisomes in the bypass of lesions, pol IV is rarely fo

168 is process was mediated by the mitochondrial replisome independent of canonical DSB repair.

169 Given the DNA damage and replisome instability associated with loss of Mcm10 func

170 ucidated, its mechanism of DNA unwinding and replisome interactions remain poorly understood.

171 erminal DHH domain, which appears poised for replisome interactions.

172 Hence, the key trigger for these replisome-intrinsic responses is cessation of leading-st

173 e inherent risk of genome instability, since replisomes invariably encounter DNA lesions or other str

174 The eukaryotic replisome is a molecular machine that coordinates the Cd

175 The replisome is a multiprotein machine responsible for the

176 The replisome is a multiprotein machine that carries out DNA

177 The replisome is a multiprotein machine that is responsible

178 The replisome is a protein complex on the DNA replication fo

179 ds, we demonstrate that the bacteriophage T7 replisome is able to directly replicate through a leadin

180 The B. subtilis replisome is eukaryotic-like in that it relies on a two

181 These observations suggest that the T7 replisome is fundamentally permissive of DNA lesions via

182 Nevertheless, the replisome is highly resistant to dilution in the absence

183 The replisome is important for DNA replication checkpoint ac

184 Furthermore, the replisome is only transiently blocked, and continues rep

185 ial component of the human mitochondrial DNA replisome is the ring-shaped helicase TWINKLE-a phage T7

186 The function of the resulting replisomes is monitored by checkpoint proteins that prot

187 In Escherichia coli, a single pair of replisomes is responsible for duplicating the entire 4.6

188 icase, CMG, demonstrating that budding yeast replisomes lack intrinsic mechanisms that control helica

189 ex acts in a dynamic fashion with the moving replisome, leading to alternating phases of slow and fas

190 ascent leading-strand gaps were generated by replisome lesion skipping.

191 n the leading-strand ssDNA gaps generated by replisome lesion skipping.

192 By analyzing chromosome and replisome localization, we demonstrated that chromosome

193 unts as well as mtDNA replication, affecting replisome machinery.

194 RecF foci frequently colocalize with replisome markers.

195 In contrast, after DNA damage, the replisome may disassemble, exposing DNA to breaks and th

196 ng proteins associated with helicases in the replisome may have coevolved with helicases to increase

197 This unexpected malleability of the replisome might allow it to deal with barriers and resou

198 The Ctf4-coupled-sister replisome model is consistent with cellular microscopy s

199 Escherichia coli replisome movement along transcribed DNA is promoted by

200 nsures that stalled forks remain stable when replisome movement is impeded.

201 In contrast, DinG does not promote replisome movement through stalled transcription complex

202 ading- and lagging-strand polymerases in the replisome must be coordinated to avoid the formation of

203 The eukaryotic replisome must faithfully replicate DNA and cope with re

204 Replisomes must be reloaded under these circumstances to

205 chinery responsible for DNA replication, the replisome, must efficiently coordinate DNA unwinding wit

206 Additionally, we found that replisome mutations that disrupt inheritance of H3-H4 te

207 Ctf4 trimer hub and the first look at a core replisome of 20 different proteins containing the helica

208 ture of the approximately 650-kDa functional replisome of bacteriophage T7 assembled on DNA resemblin

209 ng, we studied the effect of UV light on the replisome of Escherichia coli Surprisingly, our results

210 activity and inhibited DNA synthesis by the replisomes of E. coli and T7 in the presence of thioredo

211 However, replisomes often encounter obstacles, including bulky DN

212 le processivity to the high stability of the replisome once assembled on DNA.

213 instance, pol zeta is also employed when the replisome operates sub-optimally or at difficult-to-repl

214 is and revealing an underlying plasticity in replisome operation.

215 i occur infrequently, rarely colocalize with replisomes or RecF and are largely independent of RecR.

216 mere damage recognition by the break-induced replisome orchestrates homology-directed telomere mainte

217 The eukaryotic replisome, organized around the Cdc45-MCM-GINS (CMG) hel

218 In interphase, TRAIP helps replisomes overcome DNA interstrand crosslinks and DNA-p

219 Completion on the two-replisome plasmids requires RecBCD, but does not require

220 Our findings suggest two Rep-replisome populations in vivo: one continually associati

221 Barriers to replisome progress that require intervention by RadD or

222 ions or other barriers frequently compromise replisome progress.

223 omponents collaborate with CMG to facilitate replisome progression and maintain genome stability.

224 merases can alleviate potential obstacles to replisome progression by facilitating DNA lesion bypass,

225 Instead, the tethering of SCF(Dia2) to the replisome progression complex increases the efficiency o

226 racts with the Ctf4 and Mrc1 subunits of the replisome progression complex, which assembles around th

227 ssisting PriC-dependent reloading of DnaB if replisome progression fails.

228 structure and function, and that unhindered replisome progression is required for the faithful propa

229 in the context of chromatin, but subsequent replisome progression requires the histone chaperone FAC

230 e data suggest that PolY1 promotes efficient replisome progression through lagging-strand genes, ther

231 ondary structures at telomeres to facilitate replisome progression.

232 template engagement and release, modulating replisome progression.

233 d Csm3/Tof1 are crucial for in vivo rates of replisome progression.

234 to replication forks and the coordination of replisome progression.

235 ructs of the accessory helicase Rep and core replisome protein DnaQ in live Escherichia coli cells.

236 Here, we show that PARI, a replisome protein involved in regulating DNA repair and

237 inhibits Twinkle unwinding, suggesting other replisome proteins may be required for efficient removal

238 The association of specific replisome proteins with different types of cohesion esta

239 findings reveal how a single helicase at the replisome provides two independent ways of underpinning

240 ted to and released from a centrally located replisome, providing, to our knowledge, new insight into

241 In contrast, the dwell time of replisome-proximal molecules was ~8 s, consistent with t

242 The replisome quickly and accurately copies billions of DNA

243 hways that do not require fork adjustment or replisome reassembly.

244 nd that MutS motion slows when it enters the replisome region.

245 We demonstrate that the replisome remains stably bound after encountering a Tus-

246 The minimal reconstituted leading-strand replisome requires 24 proteins, forming the CMG helicase

247 DNA replication complexes (replisomes) routinely encounter proteins and unusual nuc

248 How the replisome senses and deals with DNA secondary structures

249 capable of dynamic movement to and from the replisome, showing that proper nucleotide binding is cri

250 The eukaryotic core replisome shows an unanticipated architecture, with one

251 ere, we show that SCF(Dia2) does not mediate replisome-specific degradation of Mrc1 and Ctf4, either

252 control DNA replication origin licensing and replisome stability thereby cell cycle progression throu

253 inct activities in checkpoint activation and replisome stability to ensure the viable completion of D

254 of the instabilities associated with the two-replisome substrates.

255 measure chromatin association of individual replisome subunits, thereby challenging the notion that

256 ir individual functions, including a role in replisome sumoylation.

257 The observed localization of RecF to the replisome supports the notion that RecF helps to maintai

258 to the DNA replication machinery (i.e., the replisome) than others.

259 Helicases are components of the cellular replisome that are essential for unwinding double-strand

260 ed, and a large subcomplex of the vertebrate replisome that includes DNA Pol epsilon is retained on D

261 Visualization in atomic detail of the replisome that performs concerted leading- and lagging-D

262 WDHD1 is a component of the replisome that regulates DNA replication.

263 otic cells, the assembly of the multi-enzyme replisomes that perform replication is divided into stag

264 as in mitosis it triggers disassembly of all replisomes that remain on chromatin.

265 Importantly, rather than stabilize the replisome, the checkpoint prevents two distinct types of

266 The replisome, the molecular machine dedicated to copying DN

267 The replisome, the multiprotein system responsible for genom

268 richia coli, the proofreading subunit of the replisome, the varepsilon exonuclease, is essential for

269 acting as a stable complex coordinating the replisome, these observations suggest a role of the heli

270 MTC) enhances the rate of the leading-strand replisome threefold.

271 hat are crucial for unimpeded passage of the replisome through various barriers and difficult to repl

272 During active DNA synthesis, the replisome tightly associates with DNA.

273 he fork protection complex in the eukaryotic replisome, Timeless, harbours in its C-terminal region a

274 plisome to demonstrate that Pif1 enables the replisome to bypass an inactive (i.e., dead) Cas9 (dCas9

275 ost significantly, Mcm10 enables CMG and the replisome to bypass blocks on the non-tracking DNA stran

276 in principle, allow DnaB and the associated replisome to continue DNA synthesis without impediment,

277 vitro reconstituted Saccharomyces cerevisiae replisome to demonstrate that Pif1 enables the replisome

278 enome integrity relies on the ability of the replisome to navigate ubiquitous DNA damage during DNA r

279 tor of CMG unloading and the response of the replisome to obstacles.

280 The ability of the replisome to seamlessly coordinate both high fidelity an

281 t Timeless contributes to the ability of the replisome to sense replication-hindering G4 formation an

282 g-strand Pol delta can be re-used within the replisome to support the synthesis of large numbers of O

283 II topoisomerases is critical for converging replisomes to complete DNA synthesis, but the pathways t

284 Duplication of mammalian genomes requires replisomes to overcome numerous impediments during passa

285 The Escherichia coli replisome transiently stalls at leading-strand template

286 nucleoprotein barriers that impair efficient replisome translocation.

287 The replisome unwinds and synthesizes DNA for genome duplica

288 that Ctf4 recruits the Chl1 helicase to the replisome via a conserved interaction motif that Chl1 sh

289 lC proteins within each cell and within each replisome, we elucidate the diffusion characteristics of

290 Using reconstituted budding yeast replisomes, we show that mutational inactivation of the

291 crofuidics, we investigate the effect on the replisome when encountering these barriers in live Esche

292 The details concerning the dynamics of the replisome when encountering these Tus-ter barriers in th

293 ied out by a multiprotein complex called the replisome, which encounters numerous obstacles to its pr

294 d demonstrate that it utilizes a specialized replisome, which underlies ALT telomere maintenance.

295 h enhanced expression of most genes encoding replisomes, which are positively regulated by estrogen/E

296 s performed by a large machine known as the 'replisome,' which is strictly regulated in a cell cycle-

297 Rep associates dynamically with the replisome with an average dwell time of 6.5 ms dependent

298 rcome these challenges, we paused converging replisomes with a site-specific barrier in Xenopus egg e

299 conclude with a brief comparison with other replisomes with emphasis on how coordinated DNA replicat

300 ation fork can be replicated directly by the replisome without the need to activate error-prone pathw