ライフサイエンスコーパス: reporter assay

1 mparable to GCAUG in a trichromatic splicing reporter assay.

2 genous IL1RA was assessed using a cell-based reporter assay.

3 oinformatic target prediction and luciferase reporter assay.

4 th genetic changes were investigated using a reporter assay.

5 demonstrate partial to full activity in the reporter assay.

6 ction, as measured by the beta-galactosidase reporter assay.

7 esponse to exogenous IL1beta in a cell-based reporter assay.

8 ary mouse T cells using a massively parallel reporter assay.

9 gulatory activity using a massively parallel reporter assay.

10 tly targeted by miR-124-3p with a luciferase reporter assay.

11 sessed by RT-qPCR, ChIP assay and luciferase reporter assay.

12 t cellular cytotoxicity (ADCC) activity in a reporter assay.

13 upported by the results of a dual-luciferase reporter assay.

14 hemokine 3'-UTRs that destabilized mRNA in a reporter assay.

15 ghly conserved IEC expression in a zebrafish reporter assay.

16 -/-) cells than in wild-type (WT) cells in a reporter assay.

17 tion ability as demonstrated by a luciferase reporter assay.

18 diated RNAi suppression indicated by the GFP reporter assay.

19 rmed by Western blot analysis and luciferase reporter assay.

20 on reduced CYP11B1 promoter activity using a reporter assay.

21 tested RIFINs and abolishes signalling in a reporter assay.

22 sion level of AQP4 in an in vitro luciferase reporter assay.

23 omoter activity was measured in a luciferase reporter assay.

24 vate STAT3-binding element in the luciferase reporter assay.

25 typic changes in a human embryonic stem cell reporter assay.

26 ition in WNT/beta-catenin signaling cellular reporter assay.

27 he wild-type, as demonstrated via luciferase reporter assay.

28 R was reduced by atorvastatin in a mouse FXR reporter assay.

29 ity (ADCC) was measured by a bioluminescence reporter assay.

30 , and homologous recombination (HR) by a GFP reporter assay.

31 vity was analyzed further using a cell-based reporter assay.

32 chromatin immunoprecipitation and luciferase reporter assays.

33 ated significant allele-specific activity in reporter assays.

34 ata from embryonic stem cell differentiation reporter assays.

35 imal induction of PLPP3 promoter activity in reporter assays.

36 termined by polysome fractionation and 5'UTR-reporter assays.

37 n levels and its transcriptional activity in reporter assays.

38 urification and lambdaN-BoxB tethering-based reporter assays.

39 promoter was cloned and characterised using reporter assays.

40 LD and can activate the NF-kappaB pathway in reporter assays.

41 erentially activated by R219-mutant FOXA1 in reporter assays.

42 eased transcriptional activity in luciferase reporter assays.

43 acterized at functional level using in vitro reporter assays.

44 s finding was confirmed using the luciferase reporter assays.

45 sing exogenous transfection-based luciferase reporter assays.

46 y binds an unmethylated promoter used in the reporter assays.

47 eased translation efficiencies in luciferase reporter assays.

48 using chromatin precipitation and luciferase reporter assays.

49 resulted in decreased luciferase activity in reporter assays.

50 ting the results of chromosomally integrated reporter assays.

51 bioinformatics analyses and dual-luciferase reporter assays.

52 , chromatin immunoprecipitation and promoter reporter assays.

53 o identify enhancers previously validated in reporter assays.

54 mpens SPIN1 coactivator activity in TOPflash reporter assays.

55 lidated the functionality of this site using reporter assays.

56 p, 13 candidates were screened in luciferase reporter assays.

57 cing, and their function was tested by using reporter assays.

58 ng confirms the fidelity effects seen in our reporter assays.

59 ked with mRNA expression data and transgenic reporter assays.

60 re active than SS-RJW100 in LRH-1 luciferase reporter assays.

61 cells activated CYP2B6 promoter activity in reporter assays.

62 sion studies, flow cytometry, and luciferase reporter assays.

63 onal experiments, such as genome editing and reporter assays.

64 transcription and replication in luciferase reporter assays, a mutant that may act as a phosphomimet

65 -, pT2609-DNA-PKcs, pS1778-53BP1, RIF1 and a reporter assay activation.

66 ge by 3CL(pro) This experimentally optimized reporter assay allows for antiviral drug screening in hu

67 ts this effect, as shown in a cell-based ARE reporter assay and by qPCR with native HuR mRNA targets

68 Using dual-luciferase reporter assay and Chromatin immunoprecipitation, we dem

69 roliferation as determined by LEF luciferase reporter assay and colonic organoid proliferation, respe

70 gulate GNL3 expression using dual luciferase reporter assay and CRISPR interference experiment in hum

71 it activates gene expression in a luciferase reporter assay and following retroviral transduction.

72 inhibited HSV-1 entry via beta-galactosidase reporter assay and impaired incoming virus transport to

73 s validated kinetically using the nitrocefin reporter assay and in silico binding studies.

74 Additionally, an in vivo promoter reporter assay and motility analysis revealed a key role

75 116 regions analyzed by a Massively Parallel Reporter Assay and observed enrichment of large DeltaMGW

76 g computational network analysis, an in vivo reporter assay and physiological validation experiments.

77 S inhibitors using MEIS dependent luciferase reporter assays and analysis in the ex vivo HSC assays.

78 target GPCRs, including in vitro cell-based reporter assays and binding studies.

79 ticoagulant thrombomodulin (TM), as shown by reporter assays and chromatin immunoprecipitation.

80 latory events were assessed using luciferase reporter assays and chromatin immunoprecipitation.

81 c responses were determined using luciferase reporter assays and gene expression.

82 urately predicts translation efficiencies in reporter assays and improves alpha-1-antitrypsin express

83 ico predicted target genes were confirmed in reporter assays and in house-dust-mite (HDM) induced AAI

84 The results of dual-luciferase reporter assays and in vitro studies both indicated that

85 eration, migration, apoptosis and luciferase reporter assays and in vivo xenograft mouse models.

86 s negatively regulated by MYCN in luciferase reporter assays and its synthesis in neuroblastoma cells

87 nomic stability have traditionally relied on reporter assays and on the study of deletions of individ

88 ed with increase in transcript expression in reporter assays and primary samples.

89 UTR-seq, a combination of massively parallel reporter assays and regression models, to survey the dyn

90 Dual luciferase reporter assays and RNA electrophoretic mobility shift a

91 f human UTRN sequences flank luciferase, for reporter assays and the C2C12 cell line for utrophin wes

92 1-2-5p was confirmed using 3'-UTR luciferase reporter assays and Western blot assays.

93 ches, including filter-bonding analysis, GFP reporter assay, and biolayer interferometry we observed

94 Using a combination of bioinformatics, reporter assay, and chromatin immunoprecipitation analys

95 Bioinformatic, luciferase reporter assay, and Western blot analyses identified Rho

96 ISPR interference (CRISPRi), allele-specific reporter assays, and chromatin conformation capture, we

97 ariants, antibody-mediated cell cytotoxicity reporter assays, and Fcgamma receptor-deficient (Fcer1g(

98 mmunoblotting, quantitative RT-PCR, promoter-reporter assays, and reactive oxygen species (ROS) assay

99 were functionally validated using luciferase reporter assays, and their activity was found to be esse

100 qPCR,luciferase reporter assays, and western blot also verified the ATP

101 The results of chromosomally based reporter assays are also more reproducible and more stro

102 A longstanding concern is that reporter assays are mainly implemented on episomes, whic

103 ivate the E-cadherin gene CDH1 in a promoter reporter assay as a measure of EMT reversal.

104 assessed using different T-cell factor (TCF) reporter assays as a readout for Wnt/beta-catenin-depend

105 In summary, a reporter assay based on endogenous genes on chromosome 1

106 ngements in human cells, here we developed a reporter assay based on endogenous genes on chromosome 1

107 n response to TNF was measured by luciferase reporter assays; binding of the NF-kappaB subunit p65 in

108 complementary results that are emerging from reporter assays, biochemical measurements and CRISPR scr

109 re quantified by real-time PCR, immunoblots, reporter assays, biotin-tagged promoter pulldown with pr

110 did not alter transcription in a plant-based reporter assay, but 15 R. microsporus genes were differe

111 Using a high-throughput functional reporter assay called ASSET-seq (ASsay for Splicing usin

112 oth methods, indicating that the cannabinoid reporter assay can be used for an estimation of drug con

113 NA datasets with TFBS prediction and in vivo reporter assays can reveal tissue-specific regulatory in

114 Using reporter assays, ChIP assays, and retinoic acid receptor

115 arallel reporter assays (MPRAs), traditional reporter assays, chromatin conformation capture assays,

116 l-time polymerase chain reaction, luciferase reporter assays, chromatin immunoprecipitation, docking

117 Consistently, reporter assays combined with proteomic and immunopeptid

118 A luciferase reporter assay confirmed miR-466 binding to both MR and

119 77; RNA immunoprecipitation and a luciferase reporter assay confirmed that ilp7 and ilp8 are direct t

120 Bioinformatics analysis and luciferase reporter assay confirmed that miR-466o-3p directly targe

121 A luciferase reporter assay confirmed that the 3'-untranslated region

122 A luciferase-based reporter assay confirmed the reduced ability of p.Asp411

123 Luciferase reporter assays confirmed binding of miR-4715-3p on the

124 Luciferase reporter assays confirmed that chondrocyte enhancers cha

125 Luciferase reporter assays confirmed the interaction between miR-12

126 Luciferase reporter assays confirmed the predicted interactions bet

127 The cannabinoid reporter assay correctly classified the vast majority of

128 ntibody-dependent cell-mediated cytotoxicity reporter assay correlated strongly with protection, sugg

129 lidated the functional role of pieQTLs using reporter assays, CRISPRi, dCas9-tiling guides and Cas9-m

130 ross-population eQTLs and massively parallel reporter assay data show that RAs commonly influence gen

131 The luciferase reporter assay demonstrated binding of HvWRKY23 protein

132 A dual luciferase reporter assay demonstrated that both mutations exert a

133 Luciferase-based reporter assays demonstrated target engagement at low nM

134 Luciferase reporter assays demonstrated that high miR-375 expressio

135 Reporter assays demonstrated that rs9920291 shows alleli

136 Reporter assays demonstrated that these LTR12C elements

137 g bioinformatics analysis and TOP-/FOP-flash reporter assays, demonstrated that the activation of WNT

138 Use of the NanoLuc reporter assay described herein resulted in a considerab

139 the reverse-genetics system and minireplicon reporter assay described in this study should be of valu

140 Using a reporter assay designed to study G4-induced recombinatio

141 ailed to activate transcription from Zp in a reporter assay despite inducing accumulation of HIF-1alp

142 Our data indicate that the stable CB reporter assays detect CB receptor activation by extract

143 Finally, using the reporter assay developed, we find that the histone deace

144 High-throughput reporter assays dramatically improve our ability to assi

145 Using a luciferase reporter assay, EAP1 mutants showed a reduced ability to

146 yse elt-2 gene regulation through transgenic reporter assays, ELT-2 ChIP and characterisation of in v

147 A reporter assay employing newly defined regulatory elemen

148 By employing reporter assays, EMSA, inhibition studies, bisulphite se

149 Nevertheless, designing high-throughput reporter assay experiments such as massively parallel re

150 pha-SYN expression; while exogenous promoter-reporter assays failed to reproduce the similar outcomes

151 Here, we introduce a high-throughput mouse reporter assay for assessing the pathogenic potential of

152 escence recovery after photobleaching (FRAP) reporter assay for axonal translation, we see that trans

153 regulating ECM were evaluated by luciferase reporter assays for activator protein 1 (AP-1) and NF-ka

154 ed in 5' UTRs, we devised massively parallel reporter assays from a synthetic messenger RNA library c

155 The luciferase reporter assay further verified that the miR-103 and miR

156 an cancers using a combination of cell-based reporter assays, genome editing, flow cytometry, and imm

157 BPAF, Coum, 1-BP) of 16 compounds tested by reporter assay had estrogenic activity through mERbeta2.

158 strategies similar to L1 retrotransposition reporter assays have been developed to report replicatio

159 Many studies using reporter assays have demonstrated that 3' untranslated r

160 Functional screens based on reporter assays have previously been of insufficient thr

161 Luciferase reporter assays highlighted a 173-bp region of CXCL8/IL-

162 oyed a Piwi-interacting RNA (piRNA)-targeted reporter assay in Drosophila ovary somatic sheet (OSS) c

163 We developed a two-color fluorescence reporter assay in Escherichia coli to overcome this prob

164 However, using dual-luciferase reporter assay in SH-SY5Y cells we showed that Wnt signa

165 -LacZ transgenic mice and by RARE-Luciferase reporter assays in CD cells, and investigated how this a

166 Reporter assays in Drosophila S2 cells further revealed

167 Cell-based fluorescence reporter assays in Escherichia coli revealed that mutati

168 ChIP and reporter assays in HeLa cells with monoallelic CD177 exp

169 sgenic assays in mice and transduction-based reporter assays in human cell lines (153 enhancers in to

170 The application of massively parallel reporter assays in human induced pluripotent stem cells

171 Using a panel of reporter assays in reconstituted HEK293T/17 cells, we fo

172 an islets, lower transcriptional activity in reporter assays in rodent beta-cells (rat 832/13 and mou

173 Reporter assays in transgenic mice show that the ICR con

174 Overexpression assays in zebrafish and reporter assays in vitro indicated that 4 variants were

175 pecificity of TRUB1 using massively parallel reporter assays in which we monitored Psi levels at thou

176 Transgenic reporter assays in zebrafish confirm enhancer activities

177 d anti-miRs, immunoblotting, dual luciferase reporter assay, in vivo ubiquitination assay, chase assa

178 Bioinformatics analysis and dual-luciferase reporter assay indentified LHX6 as a direct target gene

179 purification (TRAP) and in vitro luciferase reporter assay indicate that mir-92a suppresses expressi

180 Gene reporter assays indicate that relocating Rho target elem

181 Dual luciferase reporter assay indicated that MdCCA1 but not MdCSP3 acti

182 The luciferase reporter assay indicated that the 3' untranslated region

183 RNA sequencing data and a uidA reporter assay indicated that the MybA protein functions

184 Of note, reporter assays indicated that IRF5 re-expression inhibi

185 Luciferase reporter assays indicated that this differential express

186 prediction combined with an in vivo amidase reporter assay indicates that at least 23 proteins are a

187 At the molecular level, luciferase reporter assay indicates that POGZ is a negative regulat

188 tion with low micromolar IC50s in cell-based reporter assays, inhibit Gas6-inducible motility in Axl-

189 a novel lentivirus-based massively parallel reporter assay (lentiMPRA) to directly compare the funct

190 ouse ESCs, we assayed two massively parallel reporter assay (MPRA) libraries composed of binding site

191 e designed and executed a massively parallel reporter assay (MPRA) to investigate the effect of trans

192 In the massively parallel reporter assay (MPRA), short barcodes are counted by seq

193 w silencer activity using massively parallel reporter assay (MPRA).

194 Massively parallel reporter assays (MPRA) enable systematic screening of DN

195 putational approaches and massively parallel reporter assays (MPRA) surveying hundreds of promoters a

196 t an approach integrating massively-parallel reporter assays (MPRA) with cell-type-specific epigenome

197 assay experiments such as massively parallel reporter assays (MPRAs) and similar methods remains chal

198 Massively parallel reporter assays (MPRAs) are an especially versatile tech

199 Massively parallel reporter assays (MPRAs) can measure the regulatory funct

200 Massively parallel reporter assays (MPRAs) can simultaneously measure the f

201 quences using a series of massively parallel reporter assays (MPRAs) coupled with the assay for trans

202 Massively parallel reporter assays (MPRAs) functionally screen thousands of

203 Here, we used massively parallel reporter assays (MPRAs) involving 32,115 natural and syn

204 Using massively parallel reporter assays (MPRAs) to test the activity of 2,464 ca

205 itional genetic analysis, massively parallel reporter assays (MPRAs), traditional reporter assays, ch

206 Reporter assay of MRP3 promoter (ABCC3pr) revealed that

207 We developed a robust cellular cAMP reporter assay of RXFP1 signaling in HEK293 cells transi

208 lutionary sequence analysis with a live-cell reporter assay of TDP43 phase dynamics to identify regul

209 of distance and orientation in plasmid-based reporter assays of gene expression.

210 n of the top associated miRNAs by luciferase reporter assays of glucocorticoid-mediated transrepressi

211 combined with proteome arrays and luciferase reporter assays of miR-193a-3p mimic treated cord blood

212 nesis in conjunction with massively parallel reporter assays on 20 disease-associated gene promoters

213 significantly affect coupling efficiency in reporter assays or in ribosome density genome-wide.

214 try, in situ hybridization, and a transgenic reporter assay, our results demonstrate a requirement fo

215 -binding assays and cell-based frameshifting reporter assays reveal a number of key residues within n

216 However, Massively Parallel Reporter Assays reveal that few SNVs can alter the trans

217 Dual-luciferase reporter assay revealed that bta-miR-23a directly target

218 bacteria using a riboswitch-controlled GFPuv-reporter assay revealed that each mutant had a deleterio

219 Luciferase reporter assay revealed that FXR activation inhibited th

220 Luciferase reporter assay revealed that miR-124 post-transcriptiona

221 transfection of miR-1226-3p and a luciferase reporter assay revealed the reduction of AQP5 translatio

222 Chromatin immunoprecipitation and luciferase reporter assays revealed a direct binding of NANOG to a

223 iCLIP and subsequent mRNA reporter assays revealed a function for Hnrnpa1 in the r

224 Bioinformatic analysis and luciferase reporter assays revealed that both O-GlcNAcylation trans

225 Of note, luciferase reporter assays revealed that E2F3 up-regulates CREB exp

226 Genome-wide transcriptome analysis and reporter assays revealed that NeoB specifically inhibits

227 Reporter assays revealed that promoters from different a

228 ays, we focused on E2F2, with the luciferase reporter assay revealing that it was a direct target for

229 Furthermore, Luciferase reporter assay reveals that Honokiol modestly increased

230 Using reporter assays, RNA-seq, ChIP-seq, and loss-of-function

231 is extension and, using in vitro and in vivo reporter assays, show they fall into two functional clas

232 In addition, luciferase reporter assay showed that NFATc4 directly regulated the

233 Our reporter assay showed that volatile anesthetics isoflura

234 Luciferase reporter assays showed higher transcriptional activity (

235 Importantly, reporter assays showed that Gsx2 mediates opposing outco

236 Reporter assays showed that presence of wild-type, but n

237 activity of 69 TE subfamilies by luciferase reporter assays, spanning all major TE classes, and show

238 In reporter assays, subsets of these miRNAs together repres

239 r the experimental design of high-throughput reporter assays, suggesting that the extended sequence c

240 Pase activity of DHX9, and a transcriptional reporter assay suggests Nup98 supports DHX9-stimulated t

241 nced green fluorescence protein (EGFP)-based reporter assay system that allows us to perform efficien

242 ping and with the help of a novel cell-based reporter assay system we characterized the first RhCMV e

243 deep mutational scans and massively parallel reporter assays, test thousands of sequence variants in

244 We present here a novel GFP-based reporter assay that can monitor SL1 trans-splicing in li

245 We developed a novel reporter assay that enabled identification of natural co

246 characterization, typically achieved through reporter assays that test whether a sequence can increas

247 Here, we describe a multiplexed reporter assay (the Ecotox FACTORIAL) enabling parallel

248 riod was shortened as observed by luciferase reporter assays, the levels of the CCA1alpha and CCA1bet

249 ed CRISPR/Cas9 to engineer a transcriptional reporter assay to assist prediction of TNNT2 variant pat

250 Here, we used a massively parallel reporter assay to measure the cis-regulatory consequence

251 a NanoLuc binary technology (NanoBiT)-based reporter assay to screen a small library of aryl-carbon-

252 Using high-throughput luciferase reporter assay to screen for KLF2 activators, we have id

253 Here, we have used various reporter assays to demonstrate translational readthrough

254 We used transgenic reporter assays to determine the functionality of candid

255 Here, we use massively parallel reporter assays to directly measure the transcriptional

256 We used massively parallel reporter assays to perform functional comparisons of two

257 ciated loci, we performed massively parallel reporter assays to screen candidate functional variants

258 We then used in vivo reporter assays to test the tissue-specificity of these

259 es using in vivo methods (massively parallel reporter assays) to formulate quantitative models that m

260 Using 3'UTR-luciferase reporter assays, translational regulation of TEX14 was d

261 Luciferase reporter assays using 3'-UTRs of predicted miR-509-3p ta

262 Reporter assays using USP11-WT versus a binding pocket-d

263 effectively repress Wnt pathway activity in reporter assays using Wnt target gene promoters.

264 Additionally, the outcome of the cannabinoid reporter assay was compared to the gold standard (LC-MS/

265 Finally, a 45-pathway reporter assay was performed in knockdown cells.

266 , via RNA immunoprecipitation and luciferase reporter assay, we confirmed that LOC553103 directly bin

267 By luciferase gene reporter assay, we discovered that GAS5 may act as a spo

268 Working with the Cne Prp8 intein in a reporter assay, we find that the biologically relevant d

269 Finally, using an NFAT reporter assay, we identified a polyclonal antibody that

270 By using a massively parallel reporter assay, we identified cis-regulatory sequences i

271 ombining computational modeling and the BiLC reporter assay, we identified several novel small-molecu

272 protein immunoprecipitation, and luciferase reporter assay, we investigated how rates of mRNA transl

273 sing cAMP measurements and a transcriptional reporter assay, we observed that several constrained ago

274 Using a utrophin 5'3'UTR reporter assay, we performed a high-throughput screen (H

275 Using a cell-based reporter assay, we screened C4a against a panel of both

276 etry, bioinformatics analysis and luciferase reporter assay, we showed that miR-K6-5p directly target

277 Using a luciferase reporter assay, we undertook a small-molecule high-throu

278 Using reporter assays, we confirm that several pMEIs associate

279 Here, using a panel of reporter assays, we demonstrate that mating pheromone st

280 Using luciferase reporter assays, we found that miR-495 directly targeted

281 Through quantitative proteomics and reporter assays, we found that the UBE3A(T485A) protein

282 terferon stimulating response element (ISRE) reporter assays, we identified a bis-aryl sulfonamide be

283 ed gene knockdowns, and FRET-based biosensor reporter assays, we investigated the role of PLCdelta4 i

284 Here, using luciferase-based reporter assays, we provide evidence that NRP1 regulates

285 Using GUS and GFP reporter assays, we reveal their distinct or overlapping

286 Using reporter assays, we show that LREs are both required and

287 Due to intrinsic limitations of heterologous reporter assays, we sought to develop a gene editing app

288 precipitation, cell invasion, and luciferase reporter assays; we measured gene expression, binding ac

289 genome-wide small interfering RNA screen and reporter assay were used to identify host proteins requi

290 Luciferase reporter assays were performed with HT29 cells.

291 Luciferase reporter assays were used to confirm Notch1 target gene

292 n, lentiviral overexpression, and Luciferase reporter assays were used to gain insight into the mecha

293 arget prediction modules and dual-luciferase reporter assays were used to identify potential mRNA tar

294 ncreased mRNA stability, as predicted by the reporter assay, while also markedly decreasing transcrip

295 be assessed in a high-throughput fluorescent reporter assay with rVSV-SARS-CoV-2 S, and neutralizatio

296 2, and p53 pathways was compared by in vitro reporter assays with and without the pre-extraction of P

297 Our complementary reporter assays with BDNF promoter constructs indicate t

298 thod, Reg-Seq, that links massively parallel reporter assays with mass spectrometry to produce a base

299 Luciferase reporter assays with WT and mutant 3'UTRs of CYCLIN D1 a

300 In a dual-luciferase reporter assay, WNV NS1 significantly inhibited the acti