ライフサイエンスコーパス: reverse genetic

1 ific SSP functions via peptide treatment and reverse genetics.

2 All constructs were recovered by reverse genetics.

3 ex followed by analysis of their roles using reverse genetics.

4 Y A(H1N1)pdm09 NA variants were generated by reverse genetics.

5 -CON strain was generated through the use of reverse genetics.

6 these viruses in a laboratory setting using reverse genetics.

7 e functional analysis of genetic variants in reverse genetics.

8 rotein-protein interaction was studied using reverse genetics.

9 Here we used reverse genetics(5) to remodel the interaction between S

10 Through reverse genetics, a DE gene homologous to Arabidopsis CE

11 Using a combination of reverse genetics, a TPL2 kinase inhibitor and Tpl2(-/-)

12 Using reverse genetics, a VP1 mutation (K244E) was shown to be

13 nt PRRSV with the K59A mutation generated by reverse genetics almost lost the ability to reduce STAT2

14 Reverse genetic analyses also show that phy mutants of t

15 ng Rhizobium legume symbiosis (RLS)(8) or by reverse genetic analyses of differentially expressed can

16 and gene expression in expanding leaves, and reverse genetic analyses of homologous NS1 target genes

17 stomatal movements have been investigated by reverse genetics analyses in Arabidopsis (Arabidopsis th

18 Reverse genetics analyses of PA-X substitutions conserve

19 Reverse genetics analyses showed that the ability to ind

20 Reverse genetic analysis has failed to reveal abnormal p

21 This system now allows reverse genetic analysis of nairoviruses without the nee

22 Through reverse genetic analysis of the functions of four ripeni

23 The GWAS data were used to guide reverse genetic analysis, which found effectors of ABA a

24 d regulation and have also been subjected to reverse genetic analysis.

25 wn and unknown genes to identify targets for reverse genetic analysis.

26 rate the accuracy of the predictions through reverse genetics analysis.

27 Our reverse genetic and cell biology experiments suggest tha

28 Reverse genetic and chromatin immunoprecipitation-PCR ap

29 These allow robust infection and reverse genetic and immunization studies of laboratory a

30 A combination of reverse genetics and assays with Ifnar (-/-) mouse model

31 t growth and survival was demonstrated using reverse genetics and complementation studies of h2ax mut

32 ts, specific mutation in the viral genome by reverse genetics and confocal microscopy, here we demons

33 We produced a RV-A16 inoculum using reverse genetics and determined the dose necessary to ca

34 xpressing the HA protein of H5N1 HPAIV using reverse genetics and evaluated the induction of neutrali

35 Using reverse genetics and fixing the CIV-H3N2 hemagglutinin (

36 We used reverse genetics and heterologous expression to identify

37 diverse parasite developmental stages using reverse genetics and holds great potential to identify n

38 s somatic embryo system and a combination of reverse genetics and microscopy to explore the roles of

39 gh-temporal-resolution micro-array analysis, reverse genetics and mRNA-seq.

40 , we used Arabidopsis (Arabidopsis thaliana) reverse genetics and multivariate long-term time-lapse i

41 al regulators of these 150 genes, so we used reverse genetics and pharmacologic methods to explore re

42 Reverse genetics and rescue of site-directed histidine m

43 Using reverse-genetic and chemical-genetic approaches, we dete

44 lity, the MuNoV system-with its native host, reverse genetics, and cell culture systems-will continue

45 mploy comparative genomics, transcriptomics, reverse genetics, and chemical approaches to identify pu

46 y NA in H9 virus replication was observed by reverse genetics, and recombinant H9N2 viruses with amin

47 YELLOW STRIPE-LIKE5) were further studied by reverse genetics, and their functional roles in the germ

48 -genome sequencing, providing a platform for reverse genetic applications.

49 tant strains, now available for forward- and reverse-genetic applications, and the introduction of a

50 Using a reverse genetic approach and metabolic flux analysis, we

51 Thus, this advance allows a reverse genetic approach in the axolotl and will undoubt

52 We used a reverse genetic approach to disrupt laminin expression i

53 We used a reverse genetic approach to identify suppressors of ref4

54 Importantly, through a reverse genetic approach we demonstrate that the replica

55 Taking a structure-led reverse genetic approach, in both infectious virus and s

56 By using a reverse genetic approach, we characterized the role of t

57 In a reverse genetic approach, we examined two independent he

58 role of GP shedding was investigated using a reverse genetics approach by comparing recombinant virus

59 We apply a reverse genetics approach combined with controlled labor

60 A reverse genetics approach confirmed CAT4 to be responsib

61 isolates were searched for novel CFs using a reverse genetics approach followed by phenotypic analyse

62 A reverse genetics approach investigated the function of t

63 A reverse genetics approach on genes responsive to the pri

64 s contribute to pathogenesis, we exploited a reverse genetics approach to generate CE(NiP)DeltaP2-5,

65 Here, we utilized a reverse genetics approach to generate recombinant JUNV (

66 In this study, a reverse genetics approach was used to generate infectiou

67 We took a reverse genetics approach, consisting of knock-out and c

68 We took a reverse genetics approach, using CRISPR/Cas9 to generate

69 Using a reverse genetics approach, we show that disruptions in t

70 Using a reverse genetics approach, we showed that, depending on

71 -interacting host proteins in planta using a reverse-genetics approach revealed a complex, molecular

72 Here, we used a reverse-genetics approach to understand high temperature

73 Using a reverse-genetics approach, we show that genes controllin

74 the possibility of employing similar in vivo reverse genetic approaches for the generation of other v

75 Within the past 20 years, reverse genetic approaches have allowed direct determina

76 roughput sequencing has resulted in powerful reverse genetic approaches in polyploid crops.

77 Using a combination of forward and reverse genetic approaches on this line, we show here th

78 reeding or metabolic engineering) and enable reverse genetic approaches toward understanding the phys

79 2011 and 2012 demonstrated that forward and reverse genetic approaches were feasible with Chlamydia

80 Integrative bioinformatics and reverse genetic approaches were used to identify and val

81 We combined forward and reverse genetic approaches with chromatin immunoprecipit

82 Combining reverse genetic approaches, physiological methods, yeast

83 chemical, physiological, bioinformatics, and reverse genetic approaches, we analyzed how the flux of

84 Using forward and reverse genetic approaches, we identified two mutations

85 was explored by pharmacological and forward/reverse genetic approaches.

86 mplementation and overexpression assays, and reverse genetic approaches.

87 his study, we developed, for the first time, reverse genetics approaches for the generation of recomb

88 terizations by biochemical, biophysical, and reverse genetics approaches provide a better understandi

89 These reverse genetics approaches represent an excellent tool

90 We used bioinformatics and reverse genetics approaches to study the expression and

91 Using independent forward and reverse genetics approaches, we demonstrate that PfAP2-G

92 Using pharmacological and reverse genetics approaches, we show that NAD-induced RO

93 of generating recombinant TCRV (rTCRV) using reverse genetics approaches, which paves the way to stud

94 Since 1999, various forward- and reverse-genetic approaches have uncovered nearly 200 gen

95 Using reverse-genetic approaches, we demonstrate that, along w

96 We used reverse-genetics approaches to rescue a battery of recom

97 Reverse genetics based on a tandem minus-strand compleme

98 The first successful attempts to apply reverse genetics based on the available metagenomic info

99 oaches, including site-directed mutagenesis, reverse genetics-based virus recovery, expression and ch

100 ining transcriptome and expression data with reverse genetics, biochemistry, and metabolite profiling

101 hnologies have vastly increased the power of reverse genetics but selection of candidate genes, from

102 is a model organism particularly suited for reverse genetics, but this inherent characteristic limit

103 findings have important implications for how reverse genetics can be scaled in culturable Plasmodium

104 A reverse genetics cell-based evaluation of genes linked t

105 A reverse genetics, cell-based, Good Manufacturing Practic

106 The efficacy of these reverse-genetic chemical probes has been demonstrated in

107 of these specific mutations into IAV through reverse genetics confirmed their roles in resistance to

108 In this work, we have used reverse genetics coupled to biochemical and physiologica

109 bimolecular fluorescence complementation and reverse genetics) demonstrated that the calmodulin isofo

110 Remarkably, reverse-genetics-derived cell culture-adapted PEDVAVCT12

111 Reverse genetic experiments found additional VLCFA and l

112 e Ca(2+) binding site in the viral genome by reverse genetics failed to allow recovery of viable viru

113 Although reverse genetics failed to identify a function for TBAs/

114 bat influenza H18N11 virus, we propagated a reverse genetics-generated H18N11 virus in Madin-Darby c

115 om the swine isolates in their pathogenesis, reverse genetics-generated reassortants between the swin

116 accines using inactivated wild-type virus or reverse genetics-generated vaccines containing the hemag

117 ants received a single dose of a cell-based, reverse-genetics, Good Manufacturing Practices-produced

118 ere, we describe how functional genomics and reverse genetics have contributed to our understanding o

119 Reverse genetics have facilitated genome-scale knockout

120 us from bats or to generate these viruses by reverse genetics have failed to date.

121 A combination of targeted mutagenesis and reverse genetics identified the RNA-binding region that

122 Elucidation of gene function by reverse genetics in animal models frequently is complica

123 enome-wide association studies (GWAS)-guided reverse genetics in Arabidopsis thaliana, we discovered

124 We used reverse genetics in combination with biochemical methods

125 s and, therefore, change the way we will use reverse genetics in the future.

126 Using forward and reverse genetics in zebrafish, we show that disrupting t

127 ed signaling assays, confocal microscopy and reverse genetics/in vivo infection.

128 tions with mutant viruses generated by using reverse genetics indicated that the paired mutation of r

129 e to rescue HAZV from cDNAs, thus permitting reverse genetic interrogation of the HAZV replication cy

130 Reverse genetics is a gene-driven approach that comprise

131 In reverse genetic knockout (KO) studies that aim to assign

132 This novel in vivo reverse genetics method is a potentially suitable delive

133 uman parainfluenza virus type 2 (hPIV2) by a reverse genetics method of recombinant virus production.

134 between the two proteins by biochemical and reverse genetics methods paves the way for rational drug

135 defense 1 (nad1) mutant was identified using reverse genetics methods.

136 We therefore adapted a reverse-genetics minigenome (MG) rescue system based on

137 This study describes the generation, using reverse genetics, of three different recombinant influen

138 With the use of reverse genetics on an avian H5N1 virus, we found that f

139 tended to other viruses for which convenient reverse genetics or lentiviral surface display systems a

140 on (YKOC), which has enabled high-throughput reverse genetics, phenotypic screenings and analyses of

141 Application of a CRISPR-Cas9 reverse-genetics pipeline enabled insertional mutagenesi

142 In addition, the reverse genetics platform of the PC22A strain was furthe

143 The reverse genetics process produced infectious virus that

144 In this study, we combined in vitro assays, reverse genetics, quantitative N-terminomics, transcript

145 dy, we immunized mice with whole inactivated reverse genetics reassortant (RG) viruses expressing HA

146 The vL126A mutant PRRSV generated by reverse genetics replicated at a lower rate, and the tit

147 cation, our findings also show that reovirus reverse genetics rescue is enhanced 100-fold by the NP86

148 mise as a cancer therapy, efficient reovirus reverse genetics rescue will accelerate production of re

149 opics that are discussed include Arabidopsis reverse genetic resources, stock centers, databases and

150 Comprehensive reverse genetic resources, which have been key to unders

151 mediary between functional genomics data and reverse-genetics resources for the genetic dissection of

152 2A or D62A E181A mutations into VHSV-IVb via reverse genetics resulted in viruses that replicated eff

153 Forward and reverse genetics revealed new information regarding unde

154 Reverse genetics revealed that val1 mutant seeds accumul

155 loropsis oceanica (NoDGAT2s or NoDGTTs), via reverse genetics, revealed that NoDGAT2A prefers saturat

156 libraries of mutant influenza viruses using reverse genetics (RG) and selected resistant variants in

157 oal, we first established a highly efficient reverse genetics (RG) system for AHSV serotype 1 (AHSV1)

158 stablished an efficient simplified rotavirus reverse genetics (RG) system that uses 11 T7 plasmids, e

159 e recently developed plasmid-based rotavirus reverse genetics (RG) system to generate recombinant vir

160 An entirely plasmid-based reverse genetics (RG) system was recently developed for

161 An entirely plasmid-based reverse genetics (RG) system was recently developed, ope

162 e amino acid substitution in NA generated by reverse genetics (rg) that is associated with NAI resist

163 ecombinant wild-type (WT) virus generated by reverse genetics (rg-WT): rg-H274Y > rg-WT > rg-I222T >

164 rain A/chicken/Guangdong/1/1996 generated by reverse genetics (rg; rgGD/96), A/chicken/Legok/2003 (Le

165 ssessed due to the lack of a fully tractable reverse-genetics (RG) system for rotaviruses.

166 t virus with the E119A mutation generated by reverse genetics [rg-E119A], rg-D198E, rg-I222T, rg-H274

167 Using a reverse genetic screen in yeast, we identify Cue2 as the

168 We used a reverse genetic screen to identify Recognition of XopQ 1

169 regulate neuromotor function in a Drosophila reverse genetics screen.

170 ing of barcoded mutants unlocks the power of reverse genetic screening for a malaria parasite and wil

171 n malaria parasites is hampered by a lack of reverse genetic screening methods.

172 nclude that CRISPR can be used as a powerful reverse genetic screening strategy in vivo in a vertebra

173 Previously, forward and reverse genetic screens demonstrated a requirement for p

174 neered, they can be used for high-throughput reverse genetic screens to help functionally annotate th

175 We use the Haplobank in reverse genetic screens to investigate the temporal reso

176 Both forward and reverse genetic strategies have been used intensively to

177 Using a reverse genetic strategy in A. thaliana, we identified P

178 accumulation as a basis for a combined GWAS-reverse genetic strategy revealed the broad natural vari

179 Phenome-wide association is a novel reverse genetic strategy to analyze genome-to-phenome re

180 g molecular genetic tools, a large number of reverse genetic studies have propelled the use of this m

181 Reverse genetic studies implicate both Brugia osm-9 and

182 ification of candidate genes for forward and reverse genetic studies into the molecular mechanisms of

183 AV-mediated SpCas9 genome editing can enable reverse genetic studies of gene function in the brain.

184 Previous reverse genetic studies suggested that the roles of VRN1

185 esis is a powerful tool for both forward and reverse genetics studies.

186 Here, in a reverse-genetics study with mouse hepatitis coronavirus,

187 Collectively, the reverse genetic system and reporter virus provide key re

188 Here, we exploited a reverse genetic system for an enteric CoV, porcine epide

189 We report a reverse genetic system for SARS-CoV-2.

190 Results of minigenome assays and an EBOV reverse genetic system rescue support a role for both th

191 This ZIKV reverse genetic system, together with mouse and mosquito

192 combinations at these three residues with a reverse genetics system and then investigated the molecu

193 There is no reverse genetics system available for the current epidem

194 lopment and characterization of a novel ZIKV reverse genetics system based on a 2015 isolate from Pue

195 e enhancing reovirus rescue from the current reverse genetics system by 100-fold.

196 The robust reverse genetics system described will be a valuable too

197 A newly described single-plasmid reverse genetics system for noroviruses has the potentia

198 The establishment of a plasmid only-based reverse genetics system for rotaviruses by several Japan

199 We generated a reverse genetics system for the live-attenuated MV vacci

200 A novel reverse genetics system for three representative DWV var

201 Using a reverse genetics system of a prototypic arenavirus, Pich

202 Using a reverse genetics system of a prototypic arenavirus, Pich

203 ces, we first established a highly efficient reverse genetics system that increased rescue titers by

204 We developed a reverse genetics system to create a mutant of RRV (RRV(V

205 We utilized a reverse genetics system to generate a GFP reporter virus

206 this study, we employed a helper virus-based reverse genetics system to identify NSP2 gene regions th

207 We have developed a reverse genetics system to produce live recombinant PICV

208 mmal switch in vitro, we first established a reverse genetics system to rescue UUKV with a genome clo

209 Recently, a highly efficient reverse genetics system was developed that allows geneti

210 Moreover, the reverse genetics system we constructed will be helpful f

211 hese results indicate that the BAC-based MHV reverse genetics system will be useful for studies of JH

212 This optimized henipavirus reverse genetics system will facilitate future investiga

213 This improved RV reverse genetics system will facilitate study of RV biol

214 roup GII.3 strain U201 RNA, generated from a reverse genetics system, also does not induce an IFN res

215 sing Norwalk virus stool RNA transfection, a reverse genetics system, IFN neutralization reagents, an

216 Using a reverse genetics system, recombinant hMPVs (rhMPVs) lack

217 Compared to the classical reverse genetics system, the "eight-in-one" bacmids (bcm

218 Using the Pichinde virus (PICV) reverse genetics system, we analyzed the effects of alan

219 provided by organisations in DR Congo and a reverse genetics system, we generated an authentic Ebola

220 Using the SARS-CoV reverse genetics system, we generated and characterized

221 Using a reverse genetics system, we generated recombinant RSV (r

222 Using the eight-plasmid reverse genetics system, we rescued wild-type SIV A/swin

223 Using the reverse genetics system, we show that it is possible to

224 virus (PICV) GP expression vector and a PICV reverse genetics system, we systematically characterized

225 e amino acid mutation at this site through a reverse genetics system.

226 terial artificial chromosome (BAC)-based MHV reverse genetics system.

227 o better study IDV at the molecular level, a reverse-genetics system (RGS) is urgently needed, but to

228 In summary, the reverse-genetics system and minireplicon reporter assay

229 coexisting DWV genotypes, thereby devising a reverse-genetics system for an invertebrate RNA virus qu

230 elp such investigations, we have developed a reverse-genetics system for UUKV that permits manipulati

231 Utilizing a reverse-genetics system recently developed, we report th

232 was evident after the development of the BTV reverse-genetics system that allows the introduction of

233 a minigenome replication assay and a robust reverse-genetics system that can be used to further stud

234 Here, we have developed a plasmid-based IDV reverse-genetics system that can generate infectious vir

235 We established a reverse-genetics system to recover UUKV entirely from cD

236 By using the reverse-genetics system, we identified that 4 amino acid

237 Using an RV reverse-genetics system, we show that compared to the pa

238 king advantage of a modified fully tractable reverse-genetics system.

239 nological advances in glycan binding assays, reverse genetic systems for influenza and in X-ray cryst

240 We constructed reverse genetic systems for JHM.SD and JHM.WU and, utili

241 would be facilitated by the establishment of reverse genetics systems for the genetic manipulation of

242 Finally, we outline the importance of reverse genetics systems that can swiftly characterize f

243 Therefore, the use of reverse genetics systems to engineer viruses lacking NSs

244 Here we report the development of reverse genetics systems to study STFSV replication and

245 We tested the role(s) of ICAN using reverse genetic techniques to diminish its putative ion

246 In this study we use reverse genetic techniques to target putative iron-acqui

247 Reverse genetics techniques have advanced to encompass t

248 To date, several reverse genetics techniques have been reported that harn

249 Reverse genetics techniques produced a RV-A16 inoculum t

250 We used reverse genetics techniques to replace EBOV GP with its

251 Using reverse genetics techniques, we have developed a live-at

252 loned, and inoculum virus was produced using reverse genetics techniques.

253 accines are available and the development of reverse genetic technologies has raised the prospect of

254 Furthermore, using reverse genetics technology, we evaluated its role in PE

255 MPV-C) as a bivalent vaccine candidate using reverse genetics technology.

256 strain and thermolabile LaSota strain using reverse genetics technology.

257 tial enrichment, bioinformatics analysis and reverse genetics that these RNA segments are bound to th

258 In this study, we implemented reverse genetics to address these obstacles with a multi

259 In this study, we utilized forward and reverse genetics to attempt to define which aspects of t

260 ic basis of high virulence in trout, we used reverse genetics to create chimeric VHSVs in which viral

261 hat combines existing metagenomics data with reverse genetics to engineer reagents to evaluate emerge

262 This study is the first of its kind using reverse genetics to evaluate the contribution of a C. tr

263 Here we use reverse genetics to examine the role of mutations in vir

264 genetic basis for these differences, we used reverse genetics to generate a series of reassortants of

265 in the context of the intact virus, we used reverse genetics to generate Junin viruses (Candid #1 is

266 uenced sorghum mutant resource, we performed reverse genetics to identify eight genes potentially aff

267 ion, and establish a paradigm for the use of reverse genetics to inform response activities in an out

268 We used RNA interference-based reverse genetics to inhibit the production of a structur

269 e of sigma1 conformational mobility, we used reverse genetics to introduce cysteine mutations that ca

270 We coupled bioinformatics analysis with reverse genetics to introduce mutations into RSV's negat

271 Here we employed reverse genetics to investigate the role of Arabidopsis

272 We then used reverse genetics to rescue a recombinant LCMV (rLCMV) co

273 Here, we use reverse genetics to study the role of SSP myristoylation

274 We used reverse genetics to test the role of the small calcium-b

275 RNA interference (RNAi) is a valuable reverse genetics tool used in functional genomics, but r

276 Yet, the paucity of reverse genetic tools for choanoflagellates has hampered

277 Here, by exploiting reverse genetic tools optimized for primary human blood

278 a sequenced genome and the establishment of reverse genetic tools, the monarch butterfly has emerged

279 By using biochemical and reverse genetics tools, we have obtained strong evidence

280 al and chemical mutagenesis and move towards reverse-genetic tools as targeted genome editing.

281 otein synthesis within the first 2 days of a reverse genetics transfection.

282 ted (ca) H3N8 vaccine virus was generated by reverse genetics using the wild-type (wt) hemagglutinin

283 Reverse genetics utilizing a full-length infectious clon

284 Reverse genetic viruses were generated from the classica

285 sm and LD initiation and assembly.IMPORTANCE Reverse genetics was used to generate a recombinant rota

286 The Orsay virus reverse genetics we established will serve as a fundamen

287 Here, using reverse genetics, we comprehensively defined the impact

288 Using SHFV reverse genetics, we confirmed critical roles of nsp1bet

289 By using reverse genetics, we engineered recombinant PIV5-EGFP vi

290 Using reverse genetics, we engineered Ubl mutant viruses and f

291 Using reverse genetics, we examined the roles of six selected

292 By using reverse genetics, we found that a single amino acid at p

293 Using reverse genetics, we generated a rotavirus with a phosph

294 Using MHV reverse genetics, we generated a series of mutant viruse

295 Using reverse genetics, we generated a ToV-PLP knockout recomb

296 Using reverse genetics, we have generated a lead candidate onc

297 Using CRISPR/Cas9 and RNAi reverse genetics, we show that TRH-like neuropeptides, t

298 Structure guided design and reverse genetics were used to sequentially transplant la

299 irst was the advent of molecular biology and reverse genetics, which enabled the cloning and manipula

300 oduce a live attenuated candidate vaccine by reverse genetics with the hemagglutinin and neuraminidas