ライフサイエンスコーパス: reverse genetics

1 All constructs were recovered by reverse genetics.

2 ex followed by analysis of their roles using reverse genetics.

3 Y A(H1N1)pdm09 NA variants were generated by reverse genetics.

4 -CON strain was generated through the use of reverse genetics.

5 these viruses in a laboratory setting using reverse genetics.

6 ion against influenza virus by using in vivo reverse genetics.

7 s A/Ann Arbor/6/60 (AA ca) were generated by reverse genetics.

8 and diatoms 27 (CGLD27), by biochemistry or reverse genetics.

9 e functional analysis of genetic variants in reverse genetics.

10 rotein-protein interaction was studied using reverse genetics.

11 ific SSP functions via peptide treatment and reverse genetics.

12 Using reverse genetics, 3 synergistically acting mutations wer

13 Here we used reverse genetics(5) to remodel the interaction between S

14 Through reverse genetics, a DE gene homologous to Arabidopsis CE

15 Using a combination of reverse genetics, a TPL2 kinase inhibitor and Tpl2(-/-)

16 Using reverse genetics, a VP1 mutation (K244E) was shown to be

17 nt PRRSV with the K59A mutation generated by reverse genetics almost lost the ability to reduce STAT2

18 stomatal movements have been investigated by reverse genetics analyses in Arabidopsis (Arabidopsis th

19 Reverse genetics analyses of PA-X substitutions conserve

20 Reverse genetics analyses showed that the ability to ind

21 Reverse genetics analyses suggest that the structural mo

22 rate the accuracy of the predictions through reverse genetics analysis.

23 A combination of reverse genetics and assays with Ifnar (-/-) mouse model

24 atus SUN genes was investigated by combining reverse genetics and biochemistry.

25 t growth and survival was demonstrated using reverse genetics and complementation studies of h2ax mut

26 ts, specific mutation in the viral genome by reverse genetics and confocal microscopy, here we demons

27 We produced a RV-A16 inoculum using reverse genetics and determined the dose necessary to ca

28 xpressing the HA protein of H5N1 HPAIV using reverse genetics and evaluated the induction of neutrali

29 Using reverse genetics and fixing the CIV-H3N2 hemagglutinin (

30 wer of combining phylogenetic profiling with reverse genetics and gene-regulatory studies to identify

31 We used reverse genetics and heterologous expression to identify

32 diverse parasite developmental stages using reverse genetics and holds great potential to identify n

33 s somatic embryo system and a combination of reverse genetics and microscopy to explore the roles of

34 gh-temporal-resolution micro-array analysis, reverse genetics and mRNA-seq.

35 , we used Arabidopsis (Arabidopsis thaliana) reverse genetics and multivariate long-term time-lapse i

36 al regulators of these 150 genes, so we used reverse genetics and pharmacologic methods to explore re

37 Reverse genetics and rescue of site-directed histidine m

38 tunity to introduce or reinforce concepts of reverse genetics and RNA interference, suppressor screen

39 lity, the MuNoV system-with its native host, reverse genetics, and cell culture systems-will continue

40 mploy comparative genomics, transcriptomics, reverse genetics, and chemical approaches to identify pu

41 perimental approaches including mutagenesis, reverse genetics, and growth kinetics, we found that thi

42 y NA in H9 virus replication was observed by reverse genetics, and recombinant H9N2 viruses with amin

43 YELLOW STRIPE-LIKE5) were further studied by reverse genetics, and their functional roles in the germ

44 role of GP shedding was investigated using a reverse genetics approach by comparing recombinant virus

45 We apply a reverse genetics approach combined with controlled labor

46 A reverse genetics approach confirmed CAT4 to be responsib

47 isolates were searched for novel CFs using a reverse genetics approach followed by phenotypic analyse

48 A reverse genetics approach investigated the function of t

49 A reverse genetics approach on genes responsive to the pri

50 Here we employed a targeted reverse genetics approach to generate a pmpD null mutant

51 s contribute to pathogenesis, we exploited a reverse genetics approach to generate CE(NiP)DeltaP2-5,

52 Here, we utilized a reverse genetics approach to generate recombinant JUNV (

53 We took a reverse genetics approach to investigate whether GPX3 wo

54 In this study, a reverse genetics approach was used to generate infectiou

55 A reverse genetics approach with genes responsive to the p

56 We took a reverse genetics approach, consisting of knock-out and c

57 Using a reverse genetics approach, our study clearly indicates t

58 We took a reverse genetics approach, using CRISPR/Cas9 to generate

59 Using a reverse genetics approach, we show that disruptions in t

60 Using a reverse genetics approach, we showed that, depending on

61 A reverse-genetics approach has been used to probe the mec

62 -interacting host proteins in planta using a reverse-genetics approach revealed a complex, molecular

63 Here, we used a reverse-genetics approach to understand high temperature

64 Using a reverse-genetics approach, we show that genes controllin

65 his study, we developed, for the first time, reverse genetics approaches for the generation of recomb

66 st-growing needs in functional genomics, two reverse genetics approaches have been established: web-b

67 terizations by biochemical, biophysical, and reverse genetics approaches provide a better understandi

68 These reverse genetics approaches represent an excellent tool

69 e novel and represent attractive targets for reverse genetics approaches to determine their roles in

70 We used bioinformatics and reverse genetics approaches to study the expression and

71 Using reverse genetics approaches, the NSGT1-mediated glycosyl

72 Using independent forward and reverse genetics approaches, we demonstrate that PfAP2-G

73 Using pharmacological and reverse genetics approaches, we show that NAD-induced RO

74 of generating recombinant TCRV (rTCRV) using reverse genetics approaches, which paves the way to stud

75 We used reverse-genetics approaches to rescue a battery of recom

76 Reverse genetics based on a tandem minus-strand compleme

77 The first successful attempts to apply reverse genetics based on the available metagenomic info

78 Here we exploited a reverse genetics-based replication-deficient BTV serotyp

79 Here we report the development of a novel reverse genetics-based system that allows the study of E

80 oaches, including site-directed mutagenesis, reverse genetics-based virus recovery, expression and ch

81 ining transcriptome and expression data with reverse genetics, biochemistry, and metabolite profiling

82 hnologies have vastly increased the power of reverse genetics but selection of candidate genes, from

83 is a model organism particularly suited for reverse genetics, but this inherent characteristic limit

84 findings have important implications for how reverse genetics can be scaled in culturable Plasmodium

85 A reverse genetics cell-based evaluation of genes linked t

86 A reverse genetics, cell-based, Good Manufacturing Practic

87 of these specific mutations into IAV through reverse genetics confirmed their roles in resistance to

88 In this work, we have used reverse genetics coupled to biochemical and physiologica

89 bimolecular fluorescence complementation and reverse genetics) demonstrated that the calmodulin isofo

90 Remarkably, reverse-genetics-derived cell culture-adapted PEDVAVCT12

91 We sequentially passaged reverse-genetics-derived PR8 viruses with the K165E anti

92 Each mutant was successfully recovered by reverse genetics except for the one involving Ng6, which

93 In line with this notion, reverse genetics experiments supported an essential role

94 Reverse-genetics experiments demonstrated that the N145K

95 e Ca(2+) binding site in the viral genome by reverse genetics failed to allow recovery of viable viru

96 Although reverse genetics failed to identify a function for TBAs/

97 bat influenza H18N11 virus, we propagated a reverse genetics-generated H18N11 virus in Madin-Darby c

98 om the swine isolates in their pathogenesis, reverse genetics-generated reassortants between the swin

99 accines using inactivated wild-type virus or reverse genetics-generated vaccines containing the hemag

100 ants received a single dose of a cell-based, reverse-genetics, Good Manufacturing Practices-produced

101 Although reverse genetics has been used to elucidate the function

102 ere, we describe how functional genomics and reverse genetics have contributed to our understanding o

103 Reverse genetics have facilitated genome-scale knockout

104 us from bats or to generate these viruses by reverse genetics have failed to date.

105 A combination of targeted mutagenesis and reverse genetics identified the RNA-binding region that

106 2 genes of the novel H7N9 viruses created by reverse genetics in an important mammalian host model.

107 Elucidation of gene function by reverse genetics in animal models frequently is complica

108 enome-wide association studies (GWAS)-guided reverse genetics in Arabidopsis thaliana, we discovered

109 We used reverse genetics in combination with biochemical methods

110 man disease) demonstrates the power of mouse reverse genetics in mutational analysis of human genetic

111 s and, therefore, change the way we will use reverse genetics in the future.

112 Using forward and reverse genetics in zebrafish, we show that disrupting t

113 ed signaling assays, confocal microscopy and reverse genetics/in vivo infection.

114 tions with mutant viruses generated by using reverse genetics indicated that the paired mutation of r

115 Reverse genetics is a gene-driven approach that comprise

116 Through forward and reverse genetics it was shown that AtADS2 is involved in

117 /60 ca virus were generated by plasmid-based reverse genetics (Jap/57 ca, mal/78 ca, and sw/06 ca, re

118 This novel in vivo reverse genetics method is a potentially suitable delive

119 uman parainfluenza virus type 2 (hPIV2) by a reverse genetics method of recombinant virus production.

120 between the two proteins by biochemical and reverse genetics methods paves the way for rational drug

121 defense 1 (nad1) mutant was identified using reverse genetics methods.

122 We therefore adapted a reverse-genetics minigenome (MG) rescue system based on

123 This study describes the generation, using reverse genetics, of three different recombinant influen

124 With the use of reverse genetics on an avian H5N1 virus, we found that f

125 tended to other viruses for which convenient reverse genetics or lentiviral surface display systems a

126 on (YKOC), which has enabled high-throughput reverse genetics, phenotypic screenings and analyses of

127 Application of a CRISPR-Cas9 reverse-genetics pipeline enabled insertional mutagenesi

128 le ligation-free cloning to rapidly generate reverse-genetics plasmids, which can be used for the res

129 In addition, the reverse genetics platform of the PC22A strain was furthe

130 In summary, we have developed two efficient reverse genetics platforms to facilitate functional char

131 To demonstrate the utility of the two reverse genetics platforms, two mutant alleles were isol

132 The reverse genetics process produced infectious virus that

133 Further GWAS-guided reverse genetics promises to find additional effectors o

134 In this study, we combined in vitro assays, reverse genetics, quantitative N-terminomics, transcript

135 dy, we immunized mice with whole inactivated reverse genetics reassortant (RG) viruses expressing HA

136 With reverse genetics reassortants and point mutants of the t

137 The vL126A mutant PRRSV generated by reverse genetics replicated at a lower rate, and the tit

138 cation, our findings also show that reovirus reverse genetics rescue is enhanced 100-fold by the NP86

139 mise as a cancer therapy, efficient reovirus reverse genetics rescue will accelerate production of re

140 mediary between functional genomics data and reverse-genetics resources for the genetic dissection of

141 2A or D62A E181A mutations into VHSV-IVb via reverse genetics resulted in viruses that replicated eff

142 Forward and reverse genetics revealed new information regarding unde

143 Reverse genetics revealed that val1 mutant seeds accumul

144 loropsis oceanica (NoDGAT2s or NoDGTTs), via reverse genetics, revealed that NoDGAT2A prefers saturat

145 libraries of mutant influenza viruses using reverse genetics (RG) and selected resistant variants in

146 oal, we first established a highly efficient reverse genetics (RG) system for AHSV serotype 1 (AHSV1)

147 e virus (BTV) has been studied by an in vivo reverse genetics (RG) system identifying the importance

148 stablished an efficient simplified rotavirus reverse genetics (RG) system that uses 11 T7 plasmids, e

149 e recently developed plasmid-based rotavirus reverse genetics (RG) system to generate recombinant vir

150 An entirely plasmid-based reverse genetics (RG) system was recently developed for

151 An entirely plasmid-based reverse genetics (RG) system was recently developed, ope

152 e amino acid substitution in NA generated by reverse genetics (rg) that is associated with NAI resist

153 ecombinant wild-type (WT) virus generated by reverse genetics (rg-WT): rg-H274Y > rg-WT > rg-I222T >

154 rain A/chicken/Guangdong/1/1996 generated by reverse genetics (rg; rgGD/96), A/chicken/Legok/2003 (Le

155 ssessed due to the lack of a fully tractable reverse-genetics (RG) system for rotaviruses.

156 t virus with the E119A mutation generated by reverse genetics [rg-E119A], rg-D198E, rg-I222T, rg-H274

157 regulate neuromotor function in a Drosophila reverse genetics screen.

158 Reverse genetics showed functional redundancy of NHX1 an

159 esis is a powerful tool for both forward and reverse genetics studies.

160 h linker, a finding consistent with those of reverse genetics studies.

161 Here, in a reverse-genetics study with mouse hepatitis coronavirus,

162 Technical advances in reverse-genetics, such as RNA interference (RNAi), have

163 combinations at these three residues with a reverse genetics system and then investigated the molecu

164 There is no reverse genetics system available for the current epidem

165 lopment and characterization of a novel ZIKV reverse genetics system based on a 2015 isolate from Pue

166 e enhancing reovirus rescue from the current reverse genetics system by 100-fold.

167 The robust reverse genetics system described will be a valuable too

168 We constructed a reverse genetics system for avian paramyxovirus serotype

169 A newly described single-plasmid reverse genetics system for noroviruses has the potentia

170 To this end, we developed a plasmid-based reverse genetics system for Orsay virus by creating tran

171 an paramyxoviruses (APMVs), we constructed a reverse genetics system for recovery of infectious recom

172 The establishment of a plasmid only-based reverse genetics system for rotaviruses by several Japan

173 A reverse genetics system for the highly virulent NDV stra

174 We generated a reverse genetics system for the live-attenuated MV vacci

175 A novel reverse genetics system for three representative DWV var

176 This is the first plasmid-based virus reverse genetics system in the metazoan C. elegans.

177 Here we describe a recombinant human RSV reverse genetics system in which the red fluorescent pro

178 Using a reverse genetics system of a prototypic arenavirus, Pich

179 Using a reverse genetics system of a prototypic arenavirus, Pich

180 ces, we first established a highly efficient reverse genetics system that increased rescue titers by

181 We developed a reverse genetics system to create a mutant of RRV (RRV(V

182 We utilized a reverse genetics system to generate a GFP reporter virus

183 this study, we employed a helper virus-based reverse genetics system to identify NSP2 gene regions th

184 We have developed a reverse genetics system to produce live recombinant PICV

185 A reverse genetics system to rescue infectious Lujo virus

186 mmal switch in vitro, we first established a reverse genetics system to rescue UUKV with a genome clo

187 Recently, a highly efficient reverse genetics system was developed that allows geneti

188 Moreover, the reverse genetics system we constructed will be helpful f

189 hese results indicate that the BAC-based MHV reverse genetics system will be useful for studies of JH

190 This optimized henipavirus reverse genetics system will facilitate future investiga

191 This improved RV reverse genetics system will facilitate study of RV biol

192 roup GII.3 strain U201 RNA, generated from a reverse genetics system, also does not induce an IFN res

193 sing Norwalk virus stool RNA transfection, a reverse genetics system, IFN neutralization reagents, an

194 Using a reverse genetics system, recombinant hMPVs (rhMPVs) lack

195 Compared to the classical reverse genetics system, the "eight-in-one" bacmids (bcm

196 Using the Pichinde virus (PICV) reverse genetics system, we analyzed the effects of alan

197 Using an IBV reverse genetics system, we demonstrated that the templa

198 provided by organisations in DR Congo and a reverse genetics system, we generated an authentic Ebola

199 Using the SARS-CoV reverse genetics system, we generated and characterized

200 Using a reverse genetics system, we generated recombinant RSV (r

201 Using the eight-plasmid reverse genetics system, we rescued wild-type SIV A/swin

202 Using the reverse genetics system, we show that it is possible to

203 virus (PICV) GP expression vector and a PICV reverse genetics system, we systematically characterized

204 e amino acid mutation at this site through a reverse genetics system.

205 terial artificial chromosome (BAC)-based MHV reverse genetics system.

206 nt Orsay virus, were generated by use of the reverse genetics system.

207 o better study IDV at the molecular level, a reverse-genetics system (RGS) is urgently needed, but to

208 In summary, the reverse-genetics system and minireplicon reporter assay

209 coexisting DWV genotypes, thereby devising a reverse-genetics system for an invertebrate RNA virus qu

210 elp such investigations, we have developed a reverse-genetics system for UUKV that permits manipulati

211 Utilizing a reverse-genetics system recently developed, we report th

212 was evident after the development of the BTV reverse-genetics system that allows the introduction of

213 a minigenome replication assay and a robust reverse-genetics system that can be used to further stud

214 Here, we have developed a plasmid-based IDV reverse-genetics system that can generate infectious vir

215 We established a reverse-genetics system to recover UUKV entirely from cD

216 By using the reverse-genetics system, we identified that 4 amino acid

217 Using an RV reverse-genetics system, we show that compared to the pa

218 ts, which facilitate recovery of MNV using a reverse-genetics system.

219 king advantage of a modified fully tractable reverse-genetics system.

220 would be facilitated by the establishment of reverse genetics systems for the genetic manipulation of

221 The development of reverse genetics systems for wild-type strains of CDV an

222 ic biology approaches and the development of reverse genetics systems has allowed the rapid and relia

223 Finally, we outline the importance of reverse genetics systems that can swiftly characterize f

224 Using reverse genetics systems that we have recently developed

225 Therefore, the use of reverse genetics systems to engineer viruses lacking NSs

226 Here we report the development of reverse genetics systems to study STFSV replication and

227 Reverse genetics techniques have advanced to encompass t

228 To date, several reverse genetics techniques have been reported that harn

229 Reverse genetics techniques produced a RV-A16 inoculum t

230 We used reverse genetics techniques to replace EBOV GP with its

231 Using reverse genetics techniques, we have developed a live-at

232 Using plasmid-based reverse genetics techniques, we have developed a single-

233 loned, and inoculum virus was produced using reverse genetics techniques.

234 Furthermore, using reverse genetics technology, we evaluated its role in PE

235 MPV-C) as a bivalent vaccine candidate using reverse genetics technology.

236 strain and thermolabile LaSota strain using reverse genetics technology.

237 lycoproteins B (gB) and D (gD) of ILTV using reverse genetics technology.

238 ectious laryngotracheitis virus (ILTV) using reverse genetics technology.

239 TILLING) provides a nontransgenic method for reverse genetics that is widely applicable, even in spec

240 tial enrichment, bioinformatics analysis and reverse genetics that these RNA segments are bound to th

241 Here, we show, using reverse genetics, that a death-specific protein (DSP; pr

242 Using reverse genetics, the impact on growth and polymerase ac

243 Using biochemical assays and reverse genetics, the importance of conserved nsp7 and n

244 In this study, we implemented reverse genetics to address these obstacles with a multi

245 We have used reverse genetics to assess the significance of this regi

246 In this study, we utilized forward and reverse genetics to attempt to define which aspects of t

247 ic basis of high virulence in trout, we used reverse genetics to create chimeric VHSVs in which viral

248 The combination of GWAS and reverse genetics to efficiently identify new effector ge

249 hat combines existing metagenomics data with reverse genetics to engineer reagents to evaluate emerge

250 This study is the first of its kind using reverse genetics to evaluate the contribution of a C. tr

251 Here we use reverse genetics to examine the role of mutations in vir

252 tail in infectious virus production, we used reverse genetics to generate a recombinant influenza B v

253 Here, we used reverse genetics to generate a replication-incompetent i

254 genetic basis for these differences, we used reverse genetics to generate a series of reassortants of

255 Using reverse genetics to generate isogenic viruses with mutan

256 in the context of the intact virus, we used reverse genetics to generate Junin viruses (Candid #1 is

257 uenced sorghum mutant resource, we performed reverse genetics to identify eight genes potentially aff

258 ion, and establish a paradigm for the use of reverse genetics to inform response activities in an out

259 We used RNA interference-based reverse genetics to inhibit the production of a structur

260 mmodate additional genetic sequence, we used reverse genetics to insert duplications (analogous to sy

261 e of sigma1 conformational mobility, we used reverse genetics to introduce cysteine mutations that ca

262 entify determinants of transmission, we used reverse genetics to introduce gene segments of an early

263 We coupled bioinformatics analysis with reverse genetics to introduce mutations into RSV's negat

264 Here we employed reverse genetics to investigate the role of Arabidopsis

265 g polymorphism on viral fitness, we utilized reverse genetics to produce recombinant viruses encoding

266 r-wild-type (wt) MHV phenotypes when used by reverse genetics to replace its counterpart in the MHV g

267 To this end, we utilized reverse genetics to rescue a pathogenic MACV from cloned

268 We then used reverse genetics to rescue a recombinant LCMV (rLCMV) co

269 Here, we use reverse genetics to study the role of SSP myristoylation

270 We used reverse genetics to test the role of the small calcium-b

271 RNA interference (RNAi) is a valuable reverse genetics tool used in functional genomics, but r

272 revealed several novel HR-related genes, and reverse genetics tools will allow us to understand the r

273 By using biochemical and reverse genetics tools, we have obtained strong evidence

274 otein synthesis within the first 2 days of a reverse genetics transfection.

275 mutagenesis approach combining whole-genome reverse genetics using a set of tagged transposons and i

276 ted (ca) H3N8 vaccine virus was generated by reverse genetics using the wild-type (wt) hemagglutinin

277 Reverse genetics utilizing a full-length infectious clon

278 Reverse genetics was employed to examine the role of the

279 sm and LD initiation and assembly.IMPORTANCE Reverse genetics was used to generate a recombinant rota

280 The Orsay virus reverse genetics we established will serve as a fundamen

281 Here, using reverse genetics, we comprehensively defined the impact

282 Using SHFV reverse genetics, we confirmed critical roles of nsp1bet

283 By using reverse genetics, we engineered recombinant PIV5-EGFP vi

284 Using reverse genetics, we engineered Ubl mutant viruses and f

285 Using reverse genetics, we examined the roles of six selected

286 By using reverse genetics, we found that a single amino acid at p

287 Using reverse genetics, we generated a panel of recombinant hM

288 Using reverse genetics, we generated a rotavirus with a phosph

289 Using MHV reverse genetics, we generated a series of mutant viruse

290 Using reverse genetics, we generated a ToV-PLP knockout recomb

291 Using reverse genetics, we have generated a lead candidate onc

292 Using reverse genetics, we identified 2C residues that are nec

293 Here, using reverse genetics, we show that the K165E HA mutation dra

294 Using reverse genetics, we show that the ZIFL1.1 transporter r

295 Using CRISPR/Cas9 and RNAi reverse genetics, we show that TRH-like neuropeptides, t

296 Using reverse genetics, we showed ATF-2 to be functionally req

297 Viruses constructed by reverse genetics were engineered to contain converse PB2

298 Structure guided design and reverse genetics were used to sequentially transplant la

299 irst was the advent of molecular biology and reverse genetics, which enabled the cloning and manipula

300 oduce a live attenuated candidate vaccine by reverse genetics with the hemagglutinin and neuraminidas