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1 the control) and a prototype, and SARS-CoV-2 reverse transcriptase PCR (RT-PCR) results were compared
4 was not detected by Northern blot analysis, reverse transcriptase PCR revealed that these genes are
8 stationary phase in Luria-Bertani broth and reverse transcriptase PCR (RT-PCR) with oligonucleotide
9 human umbilical vein endothelial cells, and reverse transcriptase PCR demonstrated DOCK5 siRNA reduc
10 uenza test was compared to viral culture and reverse transcriptase PCR by the use of three different
14 ere performed using immunohistochemistry and reverse transcriptase PCR and operating characteristics
16 promoter was observed by RLM-5'RACE PCR and reverse transcriptase PCR analyses during expression.
17 acteriology, immunoassays, gel-based PCR and reverse transcriptase PCR, and quantitative real-time PC
20 clinical isolates were analyzed by PCR- and reverse transcriptase PCR-based analyses, respectively.
21 Gene expression studies (lacZ reporter and reverse transcriptase PCR experiments) failed to show ev
22 liquid chromatography-mass spectrometry and reverse transcriptase PCR, including lysozyme, siderocal
27 rleukin-6 (IL-6) secretion by the MMECs, and reverse transcriptase-PCR confirmed drug-related down-re
31 y nested polymerase chain reaction (PCR) and reverse-transcriptase PCR for HHV-6 and HHV-7 and by qua
32 s antigen enzyme-linked immunosorbent assay, reverse transcriptase PCR, the heteroduplex mobility ass
33 rans has been investigated by Northern blot, reverse transcriptase PCR (RT-PCR), primer extension, an
34 ined using immunofluorescence, western blot, reverse-transcriptase PCR, chromatin immunoprecipitation
36 cimens and used as a template to amplify, by reverse transcriptase PCR (RT-PCR), gonococcal genes kno
37 from mice and analyzed histologically and by reverse transcriptase PCR; leukocytes were isolated, sti
39 f RIII/Sa mice for virus characterization by reverse transcriptase PCR (RT-PCR) cloning and sequencin
42 RL), (ii) detection of T. pallidum in CSF by reverse transcriptase PCR, or (iii) new vision loss or h
47 TFF2 expression in tissues was determined by reverse transcriptase PCR, and the inflammatory and prol
49 S rRNA, sdhC, and sdhD genes was examined by reverse transcriptase PCR which showed that these three
50 he expression of these genes was followed by reverse transcriptase PCR throughout the developmental c
52 patterns were confirmed for certain genes by reverse transcriptase PCR and enzyme-linked immunosorben
54 subset of strains that were G nontypeable by reverse transcriptase PCR and found surprisingly that tw
55 Fra-2 cDNA generated from rat osteoblasts by reverse transcriptase PCR was 95% homologous to human Fr
57 20 to 40 copies/ml) but can be quantified by reverse transcriptase PCR (RT-PCR) assays with single-co
60 BVs in 48% (44/92) of the fecal specimens by reverse transcriptase PCR (RT-PCR) and amplicon sequenci
61 etection of ompP2A and ompP2B transcripts by reverse transcriptase PCR indicated that these genes wer
63 ng cancer cell lines for WWOX alterations by reverse transcriptase-PCR, loss of heterozygosity, and m
66 xcess production of glomerular VEGF(165)b by reverse transcriptase-PCR, immunohistochemistry, and ELI
67 PR-1, FPRL-1, and FPRL-2 in SKCO-15 cells by reverse transcriptase-PCR and identified expression of a
69 the full length (TRPM2-L) was determined by reverse transcriptase-PCR, and localization of endogenou
72 KOV3, AD10, A2780 and CP70) was evaluated by reverse transcriptase-PCR, western blots and immunohisto
73 ecipitation of HuR-RNA complexes followed by reverse transcriptase-PCR analysis showed that HuR inter
74 assessed by gene array analysis, followed by reverse transcriptase-PCR confirmation for significant g
75 enome data bases, and a cDNA was isolated by reverse transcriptase-PCR using Arabidopsis genome seque
78 g a specific primer pair to amplify Nanog by reverse transcriptase-PCR, we detected the expression of
80 HT29 colon cancer cells were then probed by reverse transcriptase-PCR, Western Blot analysis, and li
82 ere confirmed in extracts of SCC-13 cells by reverse-transcriptase PCR and by western blot, and by im
84 g a combination of Ca2+ imaging, single-cell reverse transcriptase-PCR and immunostaining, we show th
87 ells by immunohistochemistry and single-cell reverse transcriptase-PCR revealed that pyramidal cells
88 ultidisciplinary approaches from single-cell reverse transcriptase-PCR, mass spectrometry, to ex vivo
90 nes were further analyzed using conventional reverse transcriptase-PCR and real-time quantitative PCR
95 f the env gene, and linkage by long-distance reverse transcriptase PCR established that these predomi
96 ected in the OmcF-deficient mutant by either reverse transcriptase PCR or Northern blot analyses.
97 omas and breast cancer as measured by either reverse transcriptase PCR or nucleic acid sequence-based
98 a-Ala NMTase were used to design primers for reverse transcriptase-PCR, and several cDNA clones were
104 ltiple assays including immunocytochemistry, reverse transcriptase PCR, microarray profiling, acetylc
107 domains of intermediate filament proteins in reverse transcriptase-PCR experiments to identify and cl
108 , all of which were validated by independent reverse-transcriptase PCR experiments, with validation r
109 esolution) time points using immune markers, reverse-transcriptase-PCR (RT-PCR), and gene array appro
111 developed and validated a one-step multiplex reverse transcriptase PCR (RT-PCR) to simultaneously det
112 atory-developed test (modified CDC 2019-nCoV reverse transcriptase PCR [RT-PCR] assay with RNA extrac
114 This study demonstrates the application of reverse transcriptase PCR and RNA interference screens a
116 ew peptides were identified by sequencing of reverse transcriptase-PCR products obtained from oral mu
117 expertise requirements limit the utility of reverse transcriptase-PCR methods for rapid diagnostics.
118 etected by either DNA microarray analysis or reverse transcriptase PCR in MCMV-infected mouse fibrobl
119 r localization using immunohistochemistry or reverse transcriptase PCR and assessed histopathology, c
120 lation being identified by flow cytometry or reverse transcriptase-PCR for T-cell receptor usage or b
121 real-time polymerase chain reaction (q-PCR), reverse-transcriptase PCR (qRT-PCR) and quantitative nuc
122 post-symptom onset or post-initial positive reverse transcriptase PCR (RT-PCR) result were 92.9% (78
123 ervals on the basis of expression profiling, reverse transcriptase-PCR, haplotypes, and sequence anal
124 is observation was verified with qualitative reverse transcriptase-PCR and ELISA in human endothelial
134 L-6, a microarray approach, and quantitative reverse transcriptase PCR (q-RT-PCR), 5 target genes (tu
137 Using Northern blotting and quantitative reverse transcriptase PCR, we measured the kinetics of e
139 w cytometry-based IFN bioassay, quantitative reverse transcriptase PCR (RT-PCR), and immunoassays.
140 ication of bacteriophage MS2 by quantitative reverse transcriptase PCR (qRT-PCR) could be improved fr
142 s was independently verified by quantitative reverse transcriptase PCR and in situ hybridization.
143 A validation was carried out by quantitative reverse transcriptase PCR in 2 different set of samples.
144 genome and TAg transcription by quantitative reverse transcriptase PCR in cultured tumor cells in vit
146 e transcripts were evaluated by quantitative reverse transcriptase PCR in PCa cell lines and circulat
147 and 30 miRNAs was confirmed by quantitative reverse transcriptase PCR in samples from set 1 and set
150 in-embedded tissues of NSCLC by quantitative reverse transcriptase PCR to determine its clinicopathol
153 NA and/or translational levels, quantitative reverse transcriptase PCR (qRT-PCR) and polysome analyse
156 , we have used GeneChips and/or quantitative reverse transcriptase PCR to study UTI89 recovered from
157 of simplex and duplex real-time quantitative reverse transcriptase PCR (qRT-PCR) assays for the detec
159 ital PCR (ddPCR), and real-time quantitative reverse transcriptase PCR (RT-qPCR) from nine human cell
160 ses were evaluated by real-time quantitative reverse transcriptase PCR and immunoassays, and the quan
163 ityper technology and real-time quantitative reverse transcriptase PCR, respectively, indicates more
164 nscript profiling and follow-up quantitative reverse transcriptase PCR (qRT-PCR), reporter fusion, an
169 enome microarray, combined with quantitative reverse transcriptase PCR (qRT-PCR) and RNA sequencing (
174 and flow cytometry, as well as quantitative reverse transcriptase-PCR analysis, we found that endoge
175 ssion results were validated by quantitative reverse transcriptase-PCR (QRT-PCR), and results at the
176 cholic acid, were determined by quantitative reverse transcriptase-PCR (RT-PCR) and immunohistochemis
179 The finding was confirmed by quantitative reverse transcriptase-PCR, western blotting, and immunoh
182 crosslink immunoprecipitation, quantitative reverse transcriptase-PCR (qRT-PCR) and immunoblot exper
183 condition and degree of injury, quantitative reverse transcriptase-PCR (qPCR) and ANOVA were used to
191 al models using flow cytometry, quantitative reverse-transcriptase PCR (qRT-PCR), and RNA-Seq for PD-
192 d gene-expression changes using quantitative reverse-transcriptase PCR (qRT-PCR), immunofluorescence,
193 a finding that was confirmed by quantitative reverse-transcriptase-PCR, immunohistochemistry (IHC), a
199 cation of both DNA and mRNA targets [in situ reverse transcriptase-PCR (RT-PCR)], from frozen or para
203 d followed intensively with a supersensitive reverse transcriptase PCR assay with a lower limit of qu
205 A fluorogenic-probe hydrolysis (TaqMan)-reverse transcriptase PCR assay for classical swine feve
206 ng pathways using DNA microarray technology, reverse transcriptase-PCR, Western blot, 3-dimensional r
212 genotype, and the vaccine-specific real-time reverse transcriptase PCR (rRT-PCR) assay described by F
213 values noted on serial qualitative real-time reverse transcriptase PCR (rRT-PCR) of respiratory speci
215 design of an internally controlled real-time reverse transcriptase PCR (rRT-PCR) that detects all fou
216 cular assays, such as conventional real-time reverse transcriptase PCR (rRT-PCR), detect total RNA in
217 retrograde dye techniques and with real-time reverse transcriptase PCR (RT-PCR) analyses of single-ne
218 We have developed and evaluated a real-time reverse transcriptase PCR (RT-PCR) assay for the detecti
221 escence antibody testing (DFA) and real-time reverse transcriptase PCR (RT-PCR) using the CDC assay.
225 e microarray results, we performed real-time reverse transcriptase PCR analyses for a selection of th
233 ensitive and specific quantitative real-time reverse transcriptase PCR assay to detect the H274Y muta
237 rral laboratories were screened by real-time reverse transcriptase PCR assays using different primers
238 Microarray data were validated by real-time reverse transcriptase PCR for genes encoding early growt
239 rmulated as a one-tube, multiplex, real-time reverse transcriptase PCR for serotype identification.
242 umigatus genome was analyzed using real-time reverse transcriptase PCR in different environmental con
244 al gene expression by quantitative real-time reverse transcriptase PCR revealed that pretreatment of
246 en assay was compared to Cepheid's real-time reverse transcriptase PCR RSV analyte-specific reagents.
257 dynamic range for our quantitative real time reverse transcriptase-PCR approach, particularly for the
258 bsence of 4E-BP1, as determined by real time reverse transcriptase-PCR assays and promoter assays for
259 d a medium-throughput quantitative real time reverse transcriptase-PCR platform for the analysis of t
265 ed the upregulation of 14 genes by real-time reverse transcriptase-PCR and confirmed Act-induced prod
267 gh glucose, which was confirmed by real-time reverse transcriptase-PCR and RNase protection analysis.
268 vels in kidney cortex, measured by real-time reverse transcriptase-PCR and Western blot analysis, res
269 r), were confirmed by quantitative real-time reverse transcriptase-PCR as up-regulated in subjects wi
273 d pandemic H1N1 virus infection by real-time reverse-transcriptase PCR or viral culture; a probable c
275 rmore, microarray and quantitative real-time reverse-transcriptase-PCR (qRT-PCR) analyses revealed de
277 anscriptome analysis, quantitative real-time reverse-transcriptase-PCR, and quantitative immunohistoc
278 subjected to diagnostic tests for BVDV--two reverse transcriptase PCR (RT-PCR) assays, two commercia
284 s measured on a population of cells by using reverse transcriptase PCR, microarrays or high-throughpu
285 mRNA levels from tissues are measured using reverse transcriptase PCR, microarray analysis or high-t
290 and blood taken for Ebola confirmation using reverse-transcriptase-PCR (RT-PCR) and for haematologica
292 ncement, we assessed cytokine production via reverse transcriptase PCR and enzyme-linked immunosorben
296 d Delta fosB probes was verified by in vitro reverse transcriptase-PCR amplification to a single frag
300 All specimens were tested for influenza with reverse-transcriptase PCR, and if the result was positiv