ライフサイエンスコーパス: reverse transcription

1 ymerase to enable RNA-DNA hybrid binding and reverse transcription.

2 r a positive nasopharyngeal test by PCR with reverse transcription.

3 capsid stabilization, nucleotide import, and reverse transcription.

4 d one that inhibits virus replication during reverse transcription.

5 e the combined fidelity of transcription and reverse transcription.

6 causal link between how CA stability affects reverse transcription.

7 nfections by mutating viral DNA and impeding reverse transcription.

8 genes, using in-droplet single-cell barcoded reverse transcription.

9 cipates primarily in the elongation phase of reverse transcription.

10 in 100% agreement with quantitative PCR with reverse transcription.

11 ng viral cDNA cytosines and interfering with reverse transcription.

12 NA at the site of insertion by target primed reverse transcription.

13 ation into viral particles and inhibition of reverse transcription.

14 reaction is the linear viral DNA produced in reverse transcription.

15 inhibited HIV-1 infection without affecting reverse transcription.

16 ucing misincorporation at these sites during reverse transcription.

17 TRIM5alpha, with the block occurring before reverse transcription.

18 here the viral DNA genome is synthesized via reverse transcription.

19 ol to a level that cannot support productive reverse transcription.

20 ently restricts HIV replication at or during reverse transcription.

21 s of a given type of RNA modification during reverse transcription, allowing for site-specific identi

22 Reverse transcription, an essential event in the HIV-1 l

23 h that combines single-cell picking, lysing, reverse transcription and digital polymerase chain react

24 rotein in the nucleus during infection(5-8), reverse transcription and disassembly of the viral core

25 oteins on single RNA strands by read-through reverse transcription and DNA sequencing.

26 ion dramatically accelerated the kinetics of reverse transcription and initiation of uncoating of the

27 ng and degree of uncoating are important for reverse transcription and integration, the molecular bas

28 plexes, which are early intermediates during reverse transcription and may represent natural A3H subs

29 CA is well established to coordinate reverse transcription and nuclear entry of the virus.

30 The HIV-1 capsid protein (CA) facilitates reverse transcription and nuclear entry of the virus.

31 in activated CD4(+) T cells to inhibit HIV-1 reverse transcription and potently block viral infectivi

32 eaction (PCR) array (n = 8) and validated by reverse transcription and quantitative PCR (n = 40).

33 pendent validation cohort using conventional reverse transcription and quantitative PCR detection.

34 We leveraged reverse transcription and real-time quantitative PCR alo

35 Steady-state kinetic analysis of reverse transcription and RNA primer extension showed th

36 is report, we integrate optically-controlled reverse transcription and single-channel monitoring of L

37 these mutations accelerated the kinetics of reverse transcription and uncoating.

38 Applying quantitative microscopy of HIV-1 reverse-transcription and pre-integration-complexes (RTC

39 acquired through a discriminatory process of reverse-transcription and recombination directed by endo

40 ick examples of various biases introduced by reverse transcription, and alert the "gene expression co

41 including utilization of known host factors, reverse transcription, and uncoating, affect the sensiti

42 Capsid permeabilization and reverse transcription are altered when N57A is incorpora

43 RNA reverse transcription-associated trap sequencing (RAT-se

44 ting that the virion-associated RHA promotes reverse transcription before the virion gains access to

45 , miR-125b down-regulation depended on HIV-1 reverse transcription but not viral DNA integration.

46 The enhancing effect on reverse transcription by IP6 and the consequences of int

47 of tRNA(Lys3), the primer for initiation of reverse transcription, can promote the kissing dimer but

48 f molecular (Southern blot hybridization and reverse-transcription cleaved amplified polymorphic sequ

49 d stability and limits APOBEC3 access to the reverse transcription complex (RTC).

50 The purified HCC EVs are subjected to reverse-transcription droplet digital PCR for quantifica

51 tly, purified recombinant human RHA promoted reverse transcription efficiency under in vitro conditio

52 A are less infectious as a result of reduced reverse transcription efficiency, demonstrating that the

53 fy the mechanism by which RHA enhances HIV-1 reverse transcription efficiency.

54 in 17 min via a portable device integrating reverse transcription, fast thermocycling (via plasmonic

55 ints to regulate the precise timing of HIV-1 reverse transcription following viral entry.

56 The initiation phase of HIV reverse transcription has features that are distinct fro

57 anistic basis of how CA stability influences reverse transcription.IMPORTANCE The human immunodeficie

58 corporation of MA-CA did not markedly affect reverse transcription in infected cells, but nuclear ent

59 netics (<5 h) and precedes the completion of reverse transcription in target cells, demonstrating tha

60 ation specificity factor 6 (CPSF6), complete reverse transcription in the nucleus before uncoating, a

61 A hallmark of the initiation step of HIV-1 reverse transcription, in which viral RNA genome is conv

62 7; P = .002) regimens; and in non-nucleoside reverse transcription inhibitor-based (1563, 95% CI 1432

63 The first structure of a reverse transcription initiation complex (RTIC) that tra

64 n (+3), the first major pausing point during reverse transcription initiation.

65 ing mechanisms of nuclear import, uncoating, reverse transcription, integration, and evasion of innat

66 Here, we quantified reverse-transcription intermediates in HIV-1-infected T

67 Reverse transcription is an essential initial step in th

68 cription in target cells, demonstrating that reverse transcription is completed in the nucleus.

69 Reverse transcription is the first step of most analyses

70 this study, we describe the development of a reverse-transcription, long-range polymerase chain react

71 nostic tests using methods such as RT-PCR or reverse transcription loop mediated isothermal amplifica

72 on pads following a pH increase for specific reverse transcription loop-mediated isothermal amplifica

73 Most studies (n = 51) used reverse transcription loop-mediated isothermal amplifica

74 eased testing, we have developed a sensitive reverse-transcription loop-mediated isothermal amplifica

75 dvantages compared with standard heat-labile reverse transcription methods.

76 gation of these two modified residues in the reverse transcription model shows that both mono- or di-

77 and adaptive immune profiling by dual color Reverse-Transcription Multiplex Ligation-dependent Probe

78 steps of HIV-1 infection, such as uncoating, reverse transcription, nuclear import, and transport to

79 to induce capsid disassembly and to inhibit reverse transcription of HIV, while the ability to inhib

80 ty of applications that include single-cycle reverse transcription of long RNAs, dimethyl sulfate mut

81 NTPs, such as the dNTPase SAMHD1 that blocks reverse transcription of retroviruses in macrophages by

82 erscores the concept that core uncoating and reverse transcription of the viral genome are coordinate

83 syndrome, requires several host factors for reverse transcription of the viral genomic RNA (gRNA) in

84 Retroviral infection involves the reverse transcription of the viral RNA genome into DNA,

85 a, whereas the G94D/G116R mutations affected reverse transcription only in the presence of IFN-beta,

86 occurs in the cytoplasm in coordination with reverse transcription or at the nuclear envelope during

87 Reverse transcription PCR (RT-PCR) and sequencing reveal

88 Our results show that quantitative reverse transcription PCR (RT-QPCR) amplification of PPR

89 The current quantitative reverse transcription PCR (RT-qPCR) assay recommended fo

90 previously described multiplex quantitative reverse transcription PCR (RT-qPCR) assays.

91 Quantitative reverse transcription PCR (RT-qPCR) is one of the most e

92 Using Quantitative reverse transcription PCR (RT-qPCR), we show that CHM mR

93 Lastly, quantitative real-time reverse transcription PCR analysis of human epithelial-d

94 ns (Zika cases) were identified by real-time reverse transcription PCR and serology in a community-ba

95 d gene expression changes using quantitative reverse transcription PCR and used the result as referen

96 CoV-2 infection cases confirmed by real-time reverse transcription PCR assay of SARS-CoV-2 RNA.

97 oviral surveillance were tested by real-time reverse transcription PCR by the Instituto Nacional de I

98 The use of molecular diagnostics, such as reverse transcription PCR or unbiased metagenomic sequen

99 on and laboratory tests, typically real-time reverse transcription PCR to detect viral RNA or rapid d

100 ion of viral RNA using RT-qPCR (quantitative reverse transcription PCR), with many commercial kits no

101 iagnosis exist, including pan-Trk IHC, FISH, reverse transcription PCR, DNA-based next-generation seq

102 sitive for Zika virus infection by real-time reverse transcription PCR, including one neonate with mi

103 from the HIV study were tested by real-time reverse transcription PCR.

104 Reverse-transcription PCR confirmed altered splicing in

105 s and adenomas were analyzed by quantitative reverse-transcription PCR, single cell RNA sequencing, a

106 xpression were quantified by droplet digital reverse transcription-PCR (ddRT-PCR), providing further

107 aryngeal swabs and evaluated by quantitative reverse transcription-PCR (qRT-PCR) subsequently confirm

108 s confirmed by PCR, sequencing, quantitative reverse transcription-PCR (qRT-PCR), and functional anal

109 and on day 14 or 35 and tested by real-time reverse transcription-PCR (rRT-PCR), IgM capture enzyme-

110 In this study, reverse transcription-PCR (RT-PCR) and fluorescence-acti

111 t performance characteristics for SARS-CoV-2 reverse transcription-PCR (RT-PCR) and highlight the imp

112 Many commercial SARS-CoV-2 reverse transcription-PCR (RT-PCR) assays have received

113 In the analytic stage, real-time reverse transcription-PCR (RT-PCR) assays remain the mol

114 However, as molecular assays such as reverse transcription-PCR (RT-PCR) have increased the se

115 Traditional reverse transcription-PCR (RT-PCR) identification of kno

116 Our laboratory currently uses two real-time reverse transcription-PCR (RT-PCR) platforms, the Roche

117 We tested 125 patients who tested reverse transcription-PCR (RT-PCR) positive for SARS-CoV

118 ome more than once, and Sanger sequencing of reverse transcription-PCR (RT-PCR) products indicates th

119 Predeparture serological and viral reverse transcription-PCR (RT-PCR) testing along with re

120 6 months old for whom routine CSF EV and PeV reverse transcription-PCR (RT-PCR) testing was performed

121 piratory syndrome coronavirus 2 (SARS-CoV-2) reverse transcription-PCR (RT-PCR) testing.

122 viral 757/3139 spliced transcripts by TaqMan reverse transcription-PCR (RT-PCR), localization of infe

123 0%), exhibiting a positive response in 24/24 reverse transcription-PCR (RT-PCR)-confirmed COVID-19 ca

124 Currently, several quantitative reverse transcription-PCR (RT-qPCR) assays are being use

125 pment and validation of a triplex real-time, reverse transcription-PCR (triplex rRT-PCR) assay for th

126 Quantitative reverse transcription-PCR and tandem mass spectrometry p

127 We developed a novel reverse transcription-PCR assay targeting HHV-6B U38, wh

128 ll agreement, respectively, with a validated reverse transcription-PCR nucleic acid amplification tes

129 rformed (for example, antigen testing versus reverse transcription-PCR testing or influenza A/B testi

130 (Ct) values provided by multiplex real-time reverse transcription-PCR testing.

131 ent- and postconvalescent-phase samples from reverse transcription-PCR-confirmed cases, including 25

132 chemistry, polychromatic flow cytometry, and reverse transcription-PCR.

133 Validation by quantitative reverse-transcription-PCR in an additional 40 patients w

134 lution and an RNA extraction step to perform reverse transcription polymerase chain reaction (PCR) is

135 capacity for specialized nucleic acid-based reverse transcription polymerase chain reaction (PCR) te

136 Microarray, quantitative reverse transcription polymerase chain reaction (qRT-PCR

137 ence, immunohistochemistry, and quantitative reverse transcription polymerase chain reaction (qRT-PCR

138 ermal amplification (LAMP), and quantitative reverse transcription polymerase chain reaction (qRT-PCR

139 Quantitative Reverse transcription polymerase chain reaction (qRT-PCR

140 g with three-dimensional analysis, real-time reverse transcription polymerase chain reaction (real-ti

141 lness (ARFI) and clinician-ordered real-time reverse transcription polymerase chain reaction (rRT-PCR

142 ess, testing influenza positive on real-time reverse transcription polymerase chain reaction (rRT-PCR

143 Group 1 patients had reverse transcription polymerase chain reaction (RT-PCR)

144 est CT for COVID-19, results of chest CT and reverse transcription polymerase chain reaction (RT-PCR)

145 PEH and staff were tested for SARS-CoV-2 by reverse transcription polymerase chain reaction (RT-PCR)

146 ples were obtained from all participants for reverse transcription polymerase chain reaction (RT-PCR)

147 rimary diagnostic tool currently employed is reverse transcription polymerase chain reaction (RT-PCR)

148 COVID-19 and correlate these with real time reverse transcription polymerase chain reaction (RT-PCR)

149 Reverse transcription polymerase chain reaction (RT-PCR)

150 nocytochemistry, kallikrein 8 (KLK8) mRNA by reverse transcription polymerase chain reaction (RT-PCR)

151 hough nucleic acid detection methods such as reverse transcription polymerase chain reaction (RT-PCR)

152 Presence of COVID-19 was confirmed by reverse transcription polymerase chain reaction (RT-PCR)

153 ied by using low-dose chest CT and real-time reverse transcription polymerase chain reaction (RT-PCR)

154 cute respiratory syndrome coronavirus 2 is a reverse transcription polymerase chain reaction (RT-PCR)

155 llowed reovirus persistence (by quantitative reverse transcription polymerase chain reaction (RT-qPCR

156 0, at one center (223 patients with positive reverse transcription polymerase chain reaction [RT-PCR]

157 also produced mRNA of sufficient purity for reverse transcription polymerase chain reaction amplific

158 We used real-time reverse transcription polymerase chain reaction analysis

159 Quantitative reverse transcription polymerase chain reaction analysis

160 t significantly up-regulated by quantitative reverse transcription polymerase chain reaction analysis

161 quantified in acute-phase serum by real-time reverse transcription polymerase chain reaction and anal

162 ion analysis was assessed using quantitative reverse transcription polymerase chain reaction and reve

163 amining expression profiles (by quantitative reverse transcription polymerase chain reaction and RNAs

164 mical staining, immunoblot, and quantitative reverse transcription polymerase chain reaction for mark

165 ographs positive for COVID-19, patients with reverse transcription polymerase chain reaction results

166 nosis is made by detection of SARS-CoV-2 via reverse transcription polymerase chain reaction testing,

167 circRNA to linRNA determined by quantitative reverse transcription polymerase chain reaction varied a

168 ere acute respiratory syndrome coronavirus 2 reverse transcription polymerase chain reaction was nega

169 mined by Western blot, RT-qPCR (quantitative reverse transcription polymerase chain reaction), ELISA,

170 analyzed by histology, immunohistochemistry, reverse transcription polymerase chain reaction, and imm

171 and spheroids were analyzed by immunoblots, reverse transcription polymerase chain reaction, and liq

172 reparations, mRNA expression was measured by reverse transcription polymerase chain reaction, and PDE

173 Western blot, quantitative reverse transcription polymerase chain reaction, immunof

174 A reverse transcription polymerase chain reaction-based im

175 that compared the odds of vaccination among reverse transcription polymerase chain reaction-confirme

176 hort of hospitalized patients with real-time reverse transcription polymerase chain reaction-confirme

177 ut disease and were analyzed by quantitative reverse transcription polymerase chain reaction.

178 lloproteinase (TIMP)-1 were obtained through reverse transcription polymerase chain reaction.

179 tometry, immunoblots, immunofluorescence, or reverse transcription polymerase chain reaction.

180 avirus G and P genotypes were assigned using reverse transcription polymerase chain reaction.

181 atory cell markers by real-time quantitative reverse transcription polymerase chain reaction.

182 tested for influenza and RSV using real-time reverse transcription polymerase chain reaction.

183 Reverse transcription polymerase chain reactions and seq

184 Using HBoV1 serology, reverse-transcription polymerase chain reaction (PCR) fo

185 uorescence, immunoblots, and/or quantitative reverse-transcription polymerase chain reaction (qRT-PCR

186 The proportion of patients with real-time reverse-transcription polymerase chain reaction (RT-PCR)

187 ty has been used in scenarios of constrained reverse-transcription polymerase chain reaction (RT-PCR)

188 bulin (Ig)G and IgM serology and traditional reverse-transcription polymerase chain reaction (RT-PCR)

189 RFI who had testing for influenza viruses by reverse-transcription polymerase chain reaction (RT-PCR)

190 Reverse-transcription polymerase chain reaction (RT-PCR)

191 Reverse-transcription polymerase chain reaction (RT-PCR)

192 We enrolled quarantined people with reverse-transcription polymerase chain reaction (RT-PCR)

193 ere acute respiratory syndrome coronavirus 2 reverse-transcription polymerase chain reaction (RT-PCR)

194 We performed reverse-transcription polymerase chain reaction analyses

195 nd 6 days postvaccination were quantified by reverse-transcription polymerase chain reaction and area

196 1mut transcript levels (TLs) by quantitative reverse-transcription polymerase chain reaction and eval

197 Swabs were tested for PeV by reverse-transcription polymerase chain reaction and geno

198 by histology, immunofluorescence, xMAP, and reverse-transcription polymerase chain reaction and orga

199 tested 126/127 residents for SARS-CoV-2 via reverse-transcription polymerase chain reaction and perf

200 Influenza reverse-transcription polymerase chain reaction and Resp

201 We designed a reverse-transcription polymerase chain reaction assay th

202 oad using cycle threshold (C(T)) values from reverse-transcription polymerase chain reaction assays a

203 s were screened using previously established reverse-transcription polymerase chain reaction assays.

204 testing is less expensive than quantitative reverse-transcription polymerase chain reaction but has

205 emiologic data were linked to EBOV real-time reverse-transcription polymerase chain reaction cycle th

206 Patients with negative results of reverse-transcription polymerase chain reaction for seve

207 etransplant blood and bone marrow samples by reverse-transcription polymerase chain reaction in 107 p

208 tumor vs nontumor tissues were quantified by reverse-transcription polymerase chain reaction in fecal

209 person linked to the chalet with a positive reverse-transcription polymerase chain reaction sample f

210 020, with COVID-19 confirmation at real-time reverse-transcription polymerase chain reaction screenin

211 patients suspected of having COVID-19, using reverse-transcription polymerase chain reaction test or

212 COVID-19 on frontal chest radiographs, with reverse-transcription polymerase chain reaction test res

213 wed normal cell counts, a negative result on reverse-transcription polymerase chain reaction testing,

214 We blindly tested 60 reverse-transcription polymerase chain reaction Zika-pos

215 llected from mice and analyzed by histology, reverse-transcription polymerase chain reaction, enzyme-

216 Indeed, we demonstrated by reverse-transcription polymerase chain reaction, flow cy

217 sis validation was performed using real-time reverse-transcription polymerase chain reaction, Western

218 esulted from routine isolation and real-time reverse-transcription polymerase chain reaction-based te

219 n this retrospective study, 48 patients with reverse-transcription polymerase chain reaction-confirme

220 We estimated VE against medically attended reverse-transcription polymerase chain reaction-confirme

221 ded 70 lung lobes of 14 patients who died of reverse-transcription polymerase chain reaction-confirme

222 s In this retrospective study, patients with reverse-transcription polymerase chain reaction-confirme

223 as detected using semiquantitative real-time reverse-transcription polymerase chain reaction.

224 onized with vs without CoPEC by quantitative reverse-transcription polymerase chain reaction.

225 er (m)RNAs in the gingiva were determined by reverse-transcription polymerase chain reaction.

226 dization assay proved to be more robust than reversed transcription polymerase chain reaction (the cu

227 ive diagnosis of SARS-CoV-2 infection is the reverse transcription- polymerase chain reaction assay (

228 quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR

229 h clinical suspicion of COVID-19 and in whom reverse transcription-polymerase chain reaction (RT-PCR)

230 Reverse transcription-polymerase chain reaction (RT-PCR)

231 Reverse transcription-polymerase chain reaction (RT-PCR)

232 a great complement to the standard method of reverse transcription-polymerase chain reaction (RT-PCR)

233 GF mRNA levels were measured by quantitative reverse transcription-polymerase chain reaction (RT-qPCR

234 RNA in agricultural soils using quantitative reverse transcription-polymerase chain reaction (RT-qPCR

235 f the viral load, determined by quantitative reverse transcription-polymerase chain reaction analysis

236 le threshold (Ct) of an Ebola virus-specific reverse transcription-polymerase chain reaction assay an

237 sed using cycle threshold (Ct) values from a reverse transcription-polymerase chain reaction assay ap

238 tory test result for SARS-CoV-2 confirmed by reverse transcription-polymerase chain reaction assay.

239 and NR4A nuclear receptors was evaluated by reverse transcription-polymerase chain reaction in 305 m

240 The primary outcome measure was a positive reverse transcription-polymerase chain reaction test for

241 Using RNAseq analysis and quantitative reverse transcription-polymerase chain reaction, we demo

242 on of inflammatory cytokines was measured by reverse transcription-polymerase chain reaction.

243 -1 recruits human tRNA(Lys3) to serve as the reverse transcription primer via an interaction between

244 ypes of primers, namely, generic or specific reverse transcription primers, specific PCR primers pair

245 uding cellular tRNAs, which are exploited as reverse transcription primers.

246 targeted RNA-seq that uses complex pools of reverse-transcription primers to enable sequencing enric

247 across a 10,000 base viral genome during the reverse transcription process.

248 ral core, prior to the accumulation of viral reverse transcription products in the target cell.

249 mRNA synthesis.IMPORTANCE The fates of HIV-1 reverse transcription products within infected cells are

250 CR, whole genome sequencing, serotyping, and reverse transcription qPCR.

251 d intended-target and off-target genes using reverse transcription quantitative PCR.

252 site densities as low as 1 Pf/uL blood using reverse transcription quantitative PCR.

253 ory markers in the lung tissue lysates using reverse transcription quantitative polymerase chain reac

254 tested for the presence of SARS-CoV-2 using reverse transcription quantitative polymerase chain reac

255 RNA expression was quantified with reverse transcription quantitative polymerase chain reac

256 -1) , 1, 2.5, 5, and 10 mM) OCCM-30 cells by reverse transcription quantitative polymerase chain reac

257 50 samples that were detected positive using reverse transcription quantitative polymerase chain reac

258 as evaluated at mRNA and protein levels with reverse transcription quantitative polymerase chain reac

259 vailable for detection and quantification is reverse transcription quantitative polymerase chain reac

260 Samples were analyzed for norovirus RNA by reverse transcription quantitative polymerase chain reac

261 n components using Western blot analysis and reverse transcription quantitative polymerase chain reac

262 secreted in supernatants were quantified by reverse-transcription quantitative polymerase chain reac

263 m nasal swabs was quantified over time using reverse-transcription quantitative polymerase chain reac

264 y immunoplaque assay; 5.1 log10 copies/mL by reverse-transcription quantitative polymerase chain reac

265 and screened for HRV and HEV using real-time reverse-transcription quantitative polymerase chain reac

266 Reverse-transcription quantitative real-time polymerase

267 ent and prepared for immunohistochemical and reverse transcription, quantitative polymerase chain rea

268 rol groups were identified by microarray and reverse transcription-quantitative PCR (qRT-PCR) in the

269 the performance with that of ultrasensitive reverse transcription-quantitative PCR (uRT-qPCR) using

270 ure, high-performance liquid chromatography, reverse transcription-quantitative PCR, and genomic sequ

271 One-step growth, reverse transcription-quantitative PCR, and Western blot

272 was confirmed using immunohistochemistry and reverse transcription-quantitative PCR.

273 Reverse transcription-quantitative polymerase chain reac

274 -specific enzyme-linked immunosorbent assay, reverse transcription-quantitative polymerase chain reac

275 le specific genes were confirmed by means of reverse transcription-quantitative polymerase chain reac

276 from a nasopharyngeal (NP) swab followed by reverse transcription-quantitative polymerase chain reac

277 Virus detection (reverse transcription-quantitative real-time PCR [RT-qPC

278 U38 transcripts were detected by RNA-seq or reverse transcription-real-time quantitative PCR (RT-qPC

279 t existing diagnostic methods, an isothermal reverse transcription-recombinase polymerase amplificati

280 itive, robust, reliable, and highly specific reverse transcription-RPA technique coupled with a later

281 This assay combined advantages of reverse transcription (RT) loop-mediated isothermal ampl

282 able for analysis by techniques that rely on reverse transcription (RT) such as RT-qPCR and RNA-Seq.

283 ediated isothermal amplification (LAMP), and reverse transcription (RT).

284 RS-CoV-2) infection by quantitative PCR with reverse transcription (RT-qPCR) typically involves bulky

285 We have devised a "chromatin-RNA in situ reverse transcription sequencing" (CRIST-seq) approach t

286 this process, we used 'chromatin RNA in situ reverse transcription sequencing' (CRIST-seq) to profile

287 Without Dbr1 activity, HIV-1 reverse transcription stalls, consistent with blockage o

288 The nucleocapsid conformation depends on the reverse transcription status of the genome, which in tur

289 the effectiveness and reproducibility of the reverse transcription step has not been evaluated.

290 eplacing the enzymatically biased RNA-to-DNA reverse transcription step of RT-qPCR with a rapid nucle

291 retroviruses and retrotransposons includes a reverse transcription step, wherein dsDNA is synthesized

292 The method requires no reverse transcription step.

293 a relatively short read length and demand a reverse-transcription step, preventing effective charact

294 der in vitro conditions that mimic the early reverse transcription steps prior to capsid core uncoati

295 lfite, leading to a deletion signature after reverse transcription, supporting the RBS-Seq report.

296 sion composed of a primer binding site and a reverse-transcription template.

297 uncoating has been shown to be important for reverse transcription, the molecular mechanisms that lin

298 (2019), is a new technology that uses reverse transcription to "write" programmed sequence cha

299 orted efficient, capsid-dependent endogenous reverse transcription to produce double-stranded DNA gen

300 otinylated nucleotide is incorporated during reverse transcription, which greatly facilitates the pro