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1 t performance characteristics for SARS-CoV-2 reverse transcription-PCR (RT-PCR) and highlight the imp
4 0%), exhibiting a positive response in 24/24 reverse transcription-PCR (RT-PCR)-confirmed COVID-19 ca
12 immunohistochemistry, Western blotting, and reverse transcription-PCR, we demonstrate the location o
13 shedding was examined via tissue culture and reverse transcription-PCR (RT-PCR) testing of gill mucus
16 as verified by subcellular fractionation and reverse transcription-PCR, single-molecule fluorescence
17 r localization with immunohistochemistry and reverse transcription-PCR and measured olivocochlear fun
24 nin esterase (HE) protein was truncated, and reverse transcription-PCR (RT-PCR) studies confirmed pre
25 te its functionality to perform both PCR and reverse-transcription PCR for lambda phage DNA and H3 in
26 The use of molecular diagnostics, such as reverse transcription PCR or unbiased metagenomic sequen
28 al records of patients with EVD confirmed by reverse transcription PCR hospitalized in the Conakry ar
31 dV species D, type 37 (HAdV-D37), we show by reverse transcription PCR and Sanger sequencing that mRN
32 RNA microarray profiling and validation by reverse transcription PCR reveals that the p53-inducible
35 ption and protein synthesis were detected by reverse transcription-PCR and detection of latency-assoc
36 ion of argonaute and Dicer was determined by reverse transcription-PCR, and expression of protein was
38 amined for the presence of MCMV IE-1 mRNA by reverse transcription-PCR (RT-PCR) with Southern analysi
42 the microarray, the results were verified by reverse transcription-PCR (RT-PCR) detection of 5 gastro
43 proteins containing the WxL domain which, by reverse transcription-PCR (RT-PCR) and genomic analyses,
44 between expression of 732 genes, measured by reverse-transcription PCR, and clinical outcome in 942 p
53 d GII genotypes, were tested by conventional reverse transcription-PCR (RT-PCR)/bidirectional sequenc
54 etect all known TBPVs, based on conventional reverse transcription-PCR (RT-PCR) with degenerate prime
55 outine immunohistochemistry, flow cytometry, reverse transcription-PCR, and immunoblotting methodolog
56 re compared to those of laboratory-developed reverse transcription PCR tests for 498 nasopharyngeal s
57 r previously described poliovirus diagnostic reverse transcription-PCR (RT-PCR) assays to a real-time
58 xpression were quantified by droplet digital reverse transcription-PCR (ddRT-PCR), providing further
59 d transcript profiling and limiting-dilution reverse transcription-PCR (RT-PCR) methodologies to expl
62 iagnosis exist, including pan-Trk IHC, FISH, reverse transcription PCR, DNA-based next-generation seq
64 on were observed, complementary results from reverse transcription-PCR experiments and gel-shift and
65 ent- and postconvalescent-phase samples from reverse transcription-PCR-confirmed cases, including 25
66 In this study, we used immunohistochemistry, reverse transcription PCR, and gene arrays to determine
69 ochemical studies of MM patient bone marrow, reverse transcription-PCR and protein analysis show that
70 We describe the development of a multiplex reverse transcription-PCR (RT-PCR) with Luminex microarr
71 ction influenza A/B virus (FluA/B) multiplex reverse transcription-PCR (RT-PCR) method that amplifies
72 Quantitative PCR and single-cell multiplex reverse transcription-PCR demonstrated message for NBCe1
73 We had recently developed a rapid multiplex reverse transcription-PCR enzyme hybridization assay (Fl
74 Moreover, we describe a sensitive nested reverse transcription-PCR (RT-PCR) assay allowing the ra
76 employed transcriptome sequencing and novel reverse transcription-PCR (RT-PCR) assays to distinguish
77 the results were compared to the results of reverse transcription-PCR (RT-PCR), direct fluorescent a
78 ome more than once, and Sanger sequencing of reverse transcription-PCR (RT-PCR) products indicates th
80 6 months old for whom routine CSF EV and PeV reverse transcription-PCR (RT-PCR) testing was performed
82 and in vivo transcription, RNA purification, reverse-transcription PCR (RT-PCR) and restriction diges
90 say for influenza virus using a quantitative reverse transcription PCR-based endpoint assessment (qPC
91 e marrow chimeras, luminex, and quantitative reverse transcription PCR assays were performed to evalu
93 on CHIKV viral replication and quantitative reverse transcription PCR was used to calculate virus yi
95 cell tumor clinical samples by quantitative reverse transcription PCR analysis revealed that overall
96 th RFS were further examined by quantitative reverse transcription PCR in 291 lung adenocarcinoma tis
97 independent set of specimens by quantitative reverse transcription PCR, we defined negative-associati
101 ancer, we developed a multiplex quantitative reverse transcription PCR method involving the purificat
102 ion of viral RNA using RT-qPCR (quantitative reverse transcription PCR), with many commercial kits no
103 analysis by downstream one-step quantitative reverse transcription PCR (qRT-PCR; SYBR Green chemistry
107 d gene expression changes using quantitative reverse transcription PCR and used the result as referen
109 Validation by comparison with quantitative reverse transcription PCR showed a high correlation coef
126 ice only infrequently, although quantitative reverse transcription-PCR (qRT-PCR) tests indicated earl
127 In situ hybridization, PCR, and quantitative reverse transcription-PCR (qRT-PCR) analyses confirm tha
128 expression microarray data and quantitative reverse transcription-PCR (qRT-PCR) showed that the glob
130 genome expression profiling and quantitative reverse transcription-PCR (qRT-PCR) to monitor the macro
131 l gene expression profiling and quantitative reverse transcription-PCR (RT-PCR) validation indicated
132 Transcriptome analysis and quantitative reverse transcription-PCR showed that the type III secre
133 (5'ppp) RNA in reporter assays, quantitative reverse transcription-PCR (RT-PCR), and IRF3 phosphoryla
134 phoretic mobility shift assays, quantitative reverse transcription-PCR, and transcriptional reporter
136 selected genes were examined by quantitative reverse transcription-PCR (qPCR) to verify microarray re
137 toxin L (SElL), as confirmed by quantitative reverse transcription-PCR (qRT-PCR) and immunoblotting.
138 ied GFP transgene expression by quantitative reverse transcription-PCR (qRT-PCR) and immunohistochemi
139 ion and assembly, as assayed by quantitative reverse transcription-PCR (qRT-PCR) and transmission ele
140 unt of latency as determined by quantitative reverse transcription-PCR (qRT-PCR) of viral DNA in tota
141 aryngeal swabs and evaluated by quantitative reverse transcription-PCR (qRT-PCR) subsequently confirm
142 CL13, and CCL19/21, as shown by quantitative reverse transcription-PCR (qRT-PCR), flow cytometry, and
143 ed by BqsR/BqsS, as measured by quantitative reverse transcription-PCR (qRT-PCR), is PA14_04180, whic
147 expression in H. pylori J99 by quantitative reverse transcription-PCR (RT-PCR), demonstrating signif
149 following genes was measured by quantitative reverse transcription-PCR (RT-PCR): S100A7, IL1B, IL17A,
150 ific expression, as revealed by quantitative reverse transcription-PCR analysis of a large panel of t
151 el of which was interrogated by quantitative reverse transcription-PCR and correlated with cell cultu
153 s were further characterized by quantitative reverse transcription-PCR, Western blot, and flow cytome
162 es employing virB-lacZ fusions, quantitative reverse transcription-PCR, and immunoblot analysis showe
163 med infrared microspectroscopy, quantitative reverse transcription-PCR of cell wall biosynthetic gene
164 re confirmed by using multiplex quantitative reverse transcription-PCR for 16 mRNA targets in an inde
165 nfirmed by protein array and/or quantitative reverse transcription-PCR to be preferentially expressed
166 wide transcriptional profiling, quantitative reverse transcription-PCR, and microRNA analyses were us
167 s confirmed by PCR, sequencing, quantitative reverse transcription-PCR (qRT-PCR), and functional anal
169 by a Yersinia 16S rRNA-specific quantitative reverse transcription-PCR and was detected later by the
170 developed a multiplex one-step quantitative reverse transcription-PCR (qRT-PCR) assay for detection
171 comparing the results with the quantitative reverse transcription-PCR method routinely used in two p
174 of mRNA levels using real-time quantitative reverse transcription-PCR (qRT-PCR) further demonstrated
176 ng DNA microarray and real-time quantitative reverse transcription-PCR (qRT-PCR); these genes include
177 urface antigen, we used var Ups quantitative reverse transcription-PCR (qRT-PCR) and sequencing with
179 speB mRNA level and decay using quantitative reverse transcription-PCR (qRT-PCR) and Northern blot an
186 ource and GlpR, consistent with quantitative reverse transcription-PCR (qRT-PCR) and enzyme activity
187 hony SP/AS, in conjunction with quantitative reverse transcription-PCR (qRT-PCR), to augment or poten
188 oductive tract, as confirmed by quantitative reverse-transcription PCR (qRT-PCR) and immunohistochemi
190 were collected and analyzed by quantitative reverse-transcription PCR and histologic and biochemical
192 s and adenomas were analyzed by quantitative reverse-transcription PCR, single cell RNA sequencing, a
194 ormal tissues were subjected to quantitative reverse-transcription PCR (quantitative RT-PCR) in 3 coh
196 ustom TALE-TFs and TALENs using quantitative reverse-transcription PCR and Surveyor nuclease, respect
199 miR-26a through microarray and quantitative reverse-transcription-PCR (qRT-PCR) experiments as an mi
201 by intron mutagenesis, and semiquantitative reverse transcription-PCR (RT-PCR) showed that iron repr
202 ptional reporter system and semiquantitative reverse transcription-PCR (RT-PCR), we demonstrated that
204 , and sigG were examined by semiquantitative reverse transcription-PCR, and the corresponding sigmaF,
206 dently in a blinded fashion using the SMART, reverse transcription-PCR (RT-PCR), antigen (Ag) testing
208 respiratory conditions using virus-specific reverse transcription-PCR (RT-PCR) assays in addition to
209 e-cell mRNA transcripts by digital, one-step reverse transcription PCR in a simple microfluidic array
211 Assembly of the sequences and subsequent reverse transcription-PCR (RT-PCR) and rapid amplificati
213 viral 757/3139 spliced transcripts by TaqMan reverse transcription-PCR (RT-PCR), localization of infe
215 a microarray screen, quantitative real-time reverse transcription PCR (qPCR) confirmed that a histor
216 has relied on complex, multi-step real-time reverse transcription PCR (RT-PCR) assays; an accurate s
218 ns (Zika cases) were identified by real-time reverse transcription PCR and serology in a community-ba
220 oviral surveillance were tested by real-time reverse transcription PCR by the Instituto Nacional de I
222 on and laboratory tests, typically real-time reverse transcription PCR to detect viral RNA or rapid d
223 lated from the MAN and single-cell real-time reverse transcription PCR used to examine gene expressio
224 sitive for Zika virus infection by real-time reverse transcription PCR, including one neonate with mi
227 transcript-selective quantitative real-time reverse transcription-PCR (Q-RT-PCR) assays for the ISG5
229 Analysis of RNA by quantitative real-time reverse transcription-PCR (qRT-PCR) confirmed that the b
230 , immunoblotting, and quantitative real-time reverse transcription-PCR (qRT-PCR) measuring csgA expre
231 In response, we developed two real-time reverse transcription-PCR (rRT-PCR) assays targeting the
232 aboratory-developed DENV multiplex real-time reverse transcription-PCR (rRT-PCR) proved more clinical
234 stic utility of an EV-D68-specific real-time reverse transcription-PCR (rRT-PCR) that was recently de
236 and on day 14 or 35 and tested by real-time reverse transcription-PCR (rRT-PCR), IgM capture enzyme-
237 Rinderpest virus (RPV), based on a real-time reverse transcription-PCR (rRT-PCR)system, was developed
240 ommunity (EPIC) study, we compared real-time reverse transcription-PCR (RT-PCR) and serology for the
242 respiratory syndrome virus (PRRSV) real-time reverse transcription-PCR (RT-PCR) assays for detection
244 6 weeks of travel were tested with real-time reverse transcription-PCR (RT-PCR) assays targeting the
245 o combine and validate HPeV and EV real-time reverse transcription-PCR (RT-PCR) detection assays with
247 Our laboratory currently uses two real-time reverse transcription-PCR (RT-PCR) platforms, the Roche
248 hen mixed with Ag-Path-ID One Step real-time reverse transcription-PCR (RT-PCR) reagents and loaded i
249 KSHV mature microRNA expression by real-time reverse transcription-PCR (RT-PCR) revealed differential
256 Hedgehog signaling as monitored by real-time reverse transcription-PCR analysis of Gli1 mRNA concentr
260 rospinal fluid and identified with real-time reverse transcription-PCR and sequencing, which also yie
262 genes as assessed by quantitative real-time reverse transcription-PCR and Western blot analyses.
264 y identified by the USDA-validated real-time reverse transcription-PCR assay targeting the matrix gen
268 ght polypeptide and microarray and real-time reverse transcription-PCR revealed decreased transcript
270 and the CDC human influenza virus real-time reverse transcription-PCR swine flu panel (CDC rRT-PCR)
271 cDNAs with an improved PV-specific real-time reverse transcription-PCR system and nucleotide sequence
273 anding of the epidemiology of IDV, real-time reverse transcription-PCR was performed on a set of 208
276 up A rotavirus RNA was detected by real-time reverse transcription-PCR, and positive samples were G a
277 pture microdissection coupled with real-time reverse transcription-PCR, we confirmed that co-downregu
281 ure was confirmed by PEDV-specific real-time reverse-transcription PCR (RT-PCR), immunofluorescence a
283 pment and validation of a triplex real-time, reverse transcription-PCR (triplex rRT-PCR) assay for th
290 We validated transcriptional changes using reverse transcription-PCR, and further immunofluorescenc
293 ression of HOXA genes was investigated using reverse transcription-PCR in primary gliomas and gliobla
294 ll agreement, respectively, with a validated reverse transcription-PCR nucleic acid amplification tes
296 rformed (for example, antigen testing versus reverse transcription-PCR testing or influenza A/B testi
298 Inclusion criteria were positive Ebola virus reverse transcription PCR (RT-PCR) test, age >/= 1 y, we
299 stics of three real-time influenza A/B virus reverse transcription-PCR (RT-PCR) assays and two real-t
300 sequence of Aspergillus oryzae together with reverse-transcription-PCR and identified a transcribed s