ライフサイエンスコーパス: reverse-transcribed

1 various intervals, and RNA was isolated and reverse transcribed.

2 d from captured tissue, DNAse I treated, and reverse transcribed.

3 strictly define which TER nucleotides can be reverse transcribed.

4 liced RNA to pgRNA that are encapsidated and reverse transcribed.

5 immediate translation template if not being reverse transcribed.

6 RNA was extracted and reverse transcribed.

7 Total RNA was isolated and reverse-transcribed.

8 is end, human reticulocyte RNA was isolated, reverse transcribed, amplified by PCR and appropriate pr

9 ease controls, and 21 healthy volunteers was reverse transcribed, amplified by polymerase chain react

10 l system for intracellular protein sorting), reverse transcribed, amplified using polymerase chain re

11 ransductance regulator (CFTR) target mRNA is reverse transcribed, amplified, detected, and quantitate

12 tissues or from laboratory strains have been reverse transcribed, amplified, then hybridized to the m

13 e to the 5' end of each transcript, which is reverse transcribed and added to the chromosome.

14 mRNA is then captured on magnetic beads, reverse transcribed and amplified by emulsion V(H):V(L)

15 In the second series of analyses, we reverse transcribed and amplified by PCR RNAs from purif

16 Viral RNAs were reverse transcribed and amplified by PCR, and the produc

17 he citrus weevil, Diaprepes abbreviatus, was reverse transcribed and amplified by PCR.

18 f the CLL B cell were detected when mRNA was reverse transcribed and amplified using c mu- and/or C a

19 After RNA extraction from plasma, HCV RNA is reverse transcribed and amplified using loop-mediated is

20 RNA from human lymphoblastoid cell lines was reverse transcribed and amplified using specific "nested

21 RNA was reverse transcribed and amplified with primers from the

22 normal donors and patients with leukemia was reverse transcribed and analyzed by polymerase chain rea

23 Samples are reverse transcribed and are then amplified by reverse tr

24 ssed pseudogenes are copies of cellular RNAs reverse transcribed and inserted into the nuclear genome

25 oson that replicates via an RNA copy that is reverse transcribed and integrated elsewhere in the plan

26 nct genes, and the resulting chimera is then reverse transcribed and integrated into the genome by th

27 These RNAs can be reverse transcribed and integrated into the genomes of n

28 (DM) and two polymyositis (PM) patients was reverse transcribed and reamplified by semi-quantitative

29 the cesium chloride-guanidine method and was reverse transcribed and semiquantitated with the polymer

30 When we reverse transcribed and sequenced the mRNA of GBE1, we f

31 RNA from KB-V1 multidrug-resistant cells was reverse-transcribed and amplified by using primers deriv

32 tein (MAVS)-dependent RNA sensing pathway or reverse-transcribed and detected via the cGAS-cGAMP-STIN

33 nd isotype-control Ab, and eluted mRNAs were reverse-transcribed and hybridized to an inflammatory-fo

34 ed reporter activity originates from a fully reverse-transcribed and integrated genome.

35 the possibility that SARS-CoV-2 RNAs can be reverse-transcribed and integrated into the DNA of human

36 in reaction (RT-qPCR)-confirmed samples were reverse-transcribed and sequenced on an Illumina MiSeq u

37 The total cellular RNA was reverse-transcribed and the complementary deoxyribonucle

38 the grey fleshfly (Neobellieria bullata) was reversed transcribed and amplified by means of PCR.

39 as extracted from lenses of 12-day-old rats, reverse transcribed, and amplified using polymerase chai

40 Total RNA was harvested, reverse transcribed, and assessed by real-time polymeras

41 ingle-cell suspensions, cells isolated, mRNA reverse transcribed, and barcoded before high-throughput

42 ed children age 3 to 18 years, was purified, reverse transcribed, and biotinylated cRNA hybridized to

43 RNA was extracted, amplified, reverse transcribed, and hybridized to rat cDNA microarr

44 IV Env-pseudotyped lentiviral vectors fused, reverse transcribed, and integrated in resting cells.

45 RNA was isolated and reverse transcribed, and reverse transcription (RT)-PCR

46 i, total RNA was extracted from the thrombi, reverse transcribed, and subjected to amplification with

47 coculture, HAEC poly (A+) RNA was isolated, reverse-transcribed, and the cDNA-amplified (PCR) for a

48 reaction, where RNAs were polyadenylated and reverse-transcribed at the same time.

49 m of LTR retrotransposition, in which RNA is reverse transcribed away from the chromosomal target sit

50 DNA (cDNA) molecules that were successfully reverse transcribed but to which a second oligonucleotid

51 uential transesterification reactions and is reverse transcribed by the associated intron-encoded pro

52 one strand of a DNA target site and is then reverse transcribed by the associated intron-encoded pro

53 directly into a DNA target site and is then reverse transcribed by the associated intron-encoded pro

54 e splices into a DNA target site and is then reverse transcribed by the intron-encoded protein.

55 directly into a DNA target site and is then reverse transcribed by the intron-encoded protein.

56 pgRNA is reverse transcribed by the viral polymerase into a minus

57 Infected Vero cell and flea RNAs were reverse transcribed by using random hexamers.

58 volves gene conversion and could occur via a reverse-transcribed cDNA intermediate.

59 ogenous immunostimulatory nucleic acids: the reverse-transcribed cDNA of endogenous retroelements.

60 embranes and probed with temporally isolated reverse transcribed cDNAs from HIV-1-infected and TNF-al

61 It is commonly accepted that the reverse-transcribed cellular RNA molecules, called retro

62 This mRNA was reverse-transcribed, cloned and sequenced, confirming th

63 ed with known copy numbers of its respective reverse-transcribed cRNA.

64 The sensitivity of detection with reverse-transcribed cRNAs is as low as 100 copies.

65 s package, modern HERV-K viruses can contain reverse-transcribed DNA (RT-DNA) genomes.

66 retroelements that produce single-stranded, reverse-transcribed DNA (RT-DNA) that is a critical part

67 etrons are bacterial immune systems that use reverse-transcribed DNA (RT-DNA) to detect phage infecti

68 ing RNA (ncRNA) that result in more abundant reverse-transcribed DNA (RT-DNA).

69 f an ancestral intron-containing gene with a reverse-transcribed DNA copy of a fully spliced mRNA.

70 ses (HBVs), which are enveloped viruses with reverse-transcribed DNA genomes, constitute the family H

71 However, early reverse-transcribed DNA products were absent in the lysa

72 fection-first, in unintegrated nuclear viral reverse-transcribed DNA, resulting in its degradation; a

73 n (IFN-I) and cytokines in response to HIV-1 reverse-transcribed DNA.

74 e damage inflicted by APOBEC3 deamination of reverse-transcribed DNA.

75 it to degrade the viral genomic RNA or viral reverse-transcribed DNA.

76 ficient cells, and that Trex1 can metabolize reverse-transcribed DNA.

77 that infect animals as they integrate their reverse-transcribed double-stranded DNA into host chromo

78 esis, in which genetic information in RNA is reverse transcribed faithfully to cDNA for all template

79 expressed during embryogenesis, VHL mRNA was reverse transcribed from 13 fetal tissues (8-10 weeks ge

80 B virus (HBV) and related hepadnaviruses is reverse transcribed from a pregenomic RNA by a viral pol

81 The devised assays can detect ERalpha cDNAs reverse transcribed from as low as 50-100 pg of total RN

82 ented by concentrations of complementary DNA reverse transcribed from messenger RNA.

83 rocessed" and "nonprocessed"; the former are reverse transcribed from mRNA (and therefore have no int

84 epair exonuclease Trex1 that metabolizes DNA reverse-transcribed from ERV.

85 hybridization to complementary DNA samples, reverse-transcribed from polyadenylated RNA obtained fro

86 We show that reverse-transcribed gene transfer can take place in an o

87 1 cytoplasmic entry until integration of the reverse transcribed genome are currently enigmatic.

88 scontinuity at the approximate center of the reverse-transcribed genome.

89 However, a large number of these reverse-transcribed genomes remain unintegrated in the n

90 lian evolution, via genomic integration of a reverse transcribed HAPSTR1 transcript, and has since be

91 erum HBsAg were assessed by (PCR) for either reverse-transcribed HBV RNA, or an intact direct repeat

92 nse EPR-1 riboprobe, and by amplification of reverse-transcribed HD RNA with EPR-1-specific primers.

93 induction by the virus, suggesting that the reverse-transcribed HIV DNA triggers the innate immune r

94 e adapted a strategy to visualize individual reverse-transcribed HIV-1 cDNA molecules and their assoc

95 The process of integrating the reverse-transcribed HIV-1 DNA into the host chromosomal

96 The mandatory integration of the reverse-transcribed HIV-1 genome into host chromatin is

97 NA template of known secondary structure was reverse transcribed in the presence of 8-oxo-dGTP, dPTP

98 nucleotide of the "donor" RNA are frequently reverse transcribed in these jumps, indicating that the

99 with the viral core structures and could be reverse-transcribed in HIV-1 virions and in virus-infect

100 lly, using a novel PCR approach, we detected reverse-transcribed, integrated proviral sequences in TA

101 infection, a template region of this RNA is reverse transcribed into an array of tandem repeats that

102 a 96-well plate, where their transcripts are reverse transcribed into cDNA and labeled with the first

103 d, examined for purity and quality, and then reverse transcribed into cDNA before being subjected to

104 using single-cell RNA-seq (sequencing of RNA reverse transcribed into cDNA) and found that neurogenes

105 ing the unnatural nucleotides is efficiently reverse transcribed into cDNA, and we develop an assay t

106 om enriched Langerhans cell preparations was reverse transcribed into cDNA.

107 ated from 200-1,000 laser-captured HCAEC and reverse transcribed into cDNA.

108 RNA was extracted and reverse transcribed into complementary DNA, which was th

109 and ddm1rdr6 mutants in which genomic RNA is reverse transcribed into complementary DNA.

110 se controls, and total RNA was extracted and reverse transcribed into complementary DNA.

111 RNA was extracted from all samples and reverse transcribed into complementary DNA; V1-V2 region

112 Then, 220 known miRNAs are reverse transcribed into corresponding cDNAs by stem-loo

113 s, based on prokaryotic retrons(1), that are reverse transcribed into DNA and integrated into the gen

114 RNA enclosed inside a capsid shell, must be reverse transcribed into double-stranded DNA and release

115 shorter species of spliced RNA, which can be reverse transcribed into double-stranded DNA before viri

116 s type 1 (HIV-1) requires that its genome be reverse transcribed into double-stranded DNA for product

117 owing cell entry, the RNA genome of HIV-1 is reverse transcribed into double-stranded DNA that ultima

118 After infection, the viral RNA is reverse transcribed into double-stranded DNA, which is t

119 , a template noncoding RNA that is partially reverse transcribed into RT-DNA, and a toxic effector.

120 XNAzymes are reverse-transcribed into cDNA using XNA-dependent DNA po

121 of the human immunodeficiency virus (HIV) is reverse-transcribed into DNA and integrated into the hos

122 directly into DNA target sites and are then reverse-transcribed into genomic DNA by the associated i

123 PolyA+ mRNA from the TGs of each group was reverse transcribed, labeled with 32P, incubated on a 11

124 ese cells of different confluence states was reverse transcribed, labeled, and used to hybridize 10K

125 For DNA microarray analysis, total RNA is reverse-transcribed, labeled by incorporating fluorescen

126 RNA was extracted, amplified, reverse-transcribed, labeled, and hybridized with whole

127 is and by cloning a portion of the cDNA from reverse transcribed MG-63 cell RNA.

128 nd 40 control individuals by PCR analysis of reverse transcribed mRNA from blood leucocytes.

129 tern could be caused by gene conversion with reverse-transcribed mRNA (i.e., retroprocessing).

130 Reverse-transcribed mRNA is PCR amplified with primers s

131 PCR amplification of reverse-transcribed mRNA using gene-specific primers dem

132 Polymerase chain reaction amplification of reverse-transcribed mRNA using murine CYP2C-specific pri

133 d polymerase chain reaction amplification of reverse-transcribed mRNA, respectively.

134 However, the majority of reverse-transcribed, nuclear-imported viral genomes rema

135 ence from its 3' UTR, we believe while being reverse transcribed onto the chromosome end.

136 on of essential 5' sequence after the RNA is reverse transcribed onto the chromosome.

137 ells with rigorous quantitative, competitive reverse transcribed PCR (QC-RT-PCR) that enabled the com

138 els of CD40L-specific mRNA, as determined by reverse transcribed PCR analysis (RT-PCR) using CD40L-ho

139 Comparison of the genomic sequence to a reverse transcribed PCR product and tomato cDNA confirme

140 By a modified reverse transcribed PCR, we found that miR-143 specifica

141 (TNF-alpha) gene expression, as detected by reverse-transcribed PCR and by enzyme-linked immunosorbe

142 Total nucleic acid was extracted from DBS, reverse transcribed, PCR amplified, and analyzed by popu

143 DNA sequencing of reverse transcribed-PCR products encompassing the corres

144 Limiting-dilution reverse-transcribed PCRs confirmed down-regulation of th

145 The mRNA transcripts are then reverse-transcribed, physically linked to their partners

146 imeric full-length HIV-1 clones derived from reverse-transcribed plasma viral RNA and proviral LTRs.

147 MDR1 gene, a single product was detected by reverse-transcribed polymerase chain reaction (RT-PCR) f

148 ses of lymphoblast mRNAs from 2 patients and reverse-transcribed polymerase chain reaction (RT-PCR) o

149 Microarray and real-time reverse-transcribed polymerase chain reaction analysis o

150 Real-time reverse-transcribed polymerase chain reaction analysis r

151 Using semiquantitative multiplex reverse-transcribed polymerase chain reaction we determi

152 than Epo-responsive cells using quantitative reverse-transcribed polymerase chain reaction.

153 1 gene expression, confirmed by quantitative reverse-transcribed polymerase chain reaction.

154 lowed by nested polymerase chain reaction or reverse-transcribed polymerase chain reaction.

155 fibroblast cells using the semi-quantitative reverse transcribed-polymerase chain reaction (RT-PCR) a

156 d for TCR Vbeta usage by mAb staining and/or reverse transcribed-polymerase chain reaction analysis,

157 is enzyme is required for the integration of reverse transcribed proviral DNA into the host cell's ge

158 This mRNA was reverse transcribed, radiolabeled, and then each complem

159 ding a P450 monooxygenase was amplified from reverse transcribed rat heart and liver total RNA by pol

160 essary and sufficient for the integration of reverse-transcribed retroviral DNA into the host cell DN

161 PCR amplification of reverse transcribed RNA determined that granulomas expre

162 nts directly from genomic DNA, as opposed to reverse transcribed RNA from muscle biopsies, we have de

163 rom several granuloma CD4+ T cell lines; yet reverse transcribed RNA from T cell-depleted, dispersed

164 Differential display of reverse transcribed RNA was used to identify novel genes

165 Semi-quantitative PCR of reverse-transcribed RNA (RT-PCR) analysis revealed that

166 Using PCR to amplify reverse-transcribed RNA from bag cell clusters, we ident

167 Direct sequencing of reverse-transcribed RNA from blood plasma from three of

168 ssed by mRNA sequencing, quantitative PCR on reverse-transcribed RNA, flow cytometry, and immunohisto

169 ker of recent nuclear import of full-length, reverse-transcribed RNA, was detected in the biopsies, s

170 o the hypothesis that they were derived from reverse-transcribed RNA.

171 containing the viral integrase (IN) and the reverse transcribed (RT) copy DNA (cDNA).

172 The ligated products are reverse-transcribed, slightly amplified and cleaved with

173 A majority of the cells reverse transcribed the HIV-1 RNA, and a minority expres

174 Once reverse transcribed, the pegRNA extension functions as a

175 dermal keratinocytes, and normal human skin, reverse transcribed to cDNA and amplified by the polymer

176 otal RNA was extracted from single utricles, reverse transcribed to cDNA and the cDNA amplified by PC

177 RNA was extracted from renal biopsies and reverse transcribed to cDNA which was used as template f

178 RNA was extracted from leukocytes and reverse transcribed to cDNA, and the PCR was amplified f

179 pylori-positive gastric biopsy specimens was reverse transcribed to cDNA.

180 RNA was isolated from the urinary cells and reverse transcribed to complementary DNA.

181 elements produce an RNA intermediate that is reverse transcribed to DNA and inserted in a new genomic

182 pgRNA is then reverse transcribed to double-stranded DNA (dsDNA) withi

183 fter infection, the retroviral RNA genome is reverse transcribed to generate a linear cDNA copy, then

184 ted, ligated to an adapter at its 3' end and reverse transcribed to introduce a non-cognate nucleotid

185 s an multicopy single-stranded DNA, which is reverse transcribed to produce multiple copies of single

186 viruses is packaged into capsids where it is reverse transcribed to yield mature DNA genomes.

187 Purified RNA is then reverse-transcribed to produce cDNA, with SHAPE-modified

188 9 kb FBN1 coding segment were amplified from reverse-transcribed total fibroblast RNA.

189 arallels mammalian adaptive immunity through reverse-transcribed vDNA circles and the systemic dissem

190 iral replication requires the integration of reverse-transcribed viral cDNA into a cell chromosome.

191 IN recognizes and cleaves the ends of reverse-transcribed viral DNA and directs its insertion

192 phase of its life cycle upon integration of reverse-transcribed viral DNA into host chromatin.

193 Integration of the reverse-transcribed viral DNA into host chromosomes is a

194 Integration of the reverse-transcribed viral DNA into the host genome is an

195 tly utilise nucleosomes for insertion of the reverse-transcribed viral DNA.

196 ion of retroviruses via deamination of newly reverse-transcribed viral DNA.

197 en B-cell mRNA from these five patients were reverse transcribed with C gamma-specific primers and th

198 The attached linear intron RNA is then reverse transcribed, yielding an intron cDNA whose free