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2 using orthogonal UPLC separation strategies (reversed phase and HILIC) in both positive and negative
3 gradient or a multifactorial combination in reversed phase and ion exchange chromatography (RPLC and
4 ve validated the credentialing platform with reversed-phase and hydrophilic interaction liquid chroma
6 upon eluent composition explains the typical reversed-phase behavior (decreasing in retention followi
9 the sample and purification of oxysterols by reversed phase C18-SPE followed by HPLC-MS/MS analysis.
10 were eluted isocratically within 5 min on a reversed-phase C18 column without interference from endo
11 duced protein digests were separated using a reversed-phase C18 column, partially reduced by post-col
12 and 3D LC-MS/MS separation protocols (all of reversed-phase C18 functionality) to a tryptic digest of
13 of the samples with solid-phase extractions: reversed-phase (C18) and strong cation-exchange (SCX).
15 ne UHPLC separation into 8 fractions using a reversed-phase C4 column led to approximately twice as m
16 arried out by binary gradient technique on a reversed-phase C8 Zorbax column and the detection was ma
18 per demonstrates for the first time that C18 reversed-phase capillary liquid chromatography (Cap-LC)
20 differences in selectivity across a range of reversed-phase chemistries, achieving the purification o
21 The method entails an aqueous extraction and reversed phase chromatographic separation using pentaflu
22 tribute to explain the retention behavior of reversed-phase chromatographic columns when used under h
23 mixture of chemicals in the AF extract with reversed-phase chromatographic fractionation and subsequ
25 iopharmaceutical characterization to enhance reversed-phase chromatographic performance of peptide se
29 ize exclusion chromatography (SEC), ion-pair reversed phase chromatography (IPRP), and hydrophilic in
30 (ACE) in the first separation dimension and reversed phase chromatography (RP) in the second separat
31 y ionizable or retained analytes amenable to reversed phase chromatography and electrospray ionizatio
32 es (aqueous extract) combined with HILIC and reversed phase chromatography and time-of-flight mass sp
35 extracts of apple peels were fractionated by reversed phase chromatography using gradient elution of
37 (salt-free) ion exchange chromatography and reversed phase chromatography-mass spectrometry allowed
39 in both identity as well as purity, ion-pair reversed-phase chromatography (IP-RP) at high temperatur
41 graphy seleno-amino acids were determined by reversed-phase chromatography (RPC) coupled to ICP-MS.
42 rial size exclusion chromatography (sSEC) to reversed-phase chromatography (RPC) expanded coverage of
44 es were isolated using cationic exchange and reversed-phase chromatography and identified by (1)H NMR
45 ample preparation, off-line fractionation by reversed-phase chromatography at pH 10, immobilization o
47 esting times were analyzed using ion-pairing reversed-phase chromatography coupled to an ICPMS/MS det
48 philic interaction liquid chromatography and reversed-phase chromatography enables the investigation
49 collected, pooled together and subjected to reversed-phase chromatography for further purification.
52 ass of cationic ion-interaction reagents for reversed-phase chromatography is introduced in the prese
55 techniques and peptide retention modeling in reversed-phase chromatography to generate a data set suf
56 hylls and bacteriochlorophylls) was based on reversed-phase chromatography with a methanol-acetone gr
58 AH was separated using ultra-filtration and reversed-phase chromatography, and assessment of the fra
59 e to separate the isomers, or who were using reversed-phase chromatography, gave rise to multi-modal
60 oxylic acid cycle (TCA cycle), by mixed-mode reversed-phase chromatography, on a CSH Phenyl-Hexyl col
62 tissue patches were directly collected on a reversed phase column and analyzed using an on-column ex
63 se, cellulase), the compounds separated on a reversed phase column by gradient elution and detected b
65 ographic separation was achieved using a C18 reversed phase column with gradient elution of basic mob
68 d detection of tryptophan are performed on a reversed-phase column with fluorescence detection within
71 sing high performance liquid chromatography (reversed phase columns, UV-Vis diode array detector) at
73 ses strong cation-exchange (SCX) followed by reversed-phase desalting to remove Ficoll, a synthetic p
74 gradient mode were performed both in common reversed-phase eluents and environmental friendly ethano
76 through solid-phase extraction (SPE) with a reversed phase functionalized polymeric sorbent and spik
77 e generation of droplets is also possible in reversed phase gradient elution mode as demonstrated by
78 ing TFA as an acid modifier to a formic acid/reversed phase gradient, providing additional resolving
80 embranes, cation exchange chromatography and reversed phase high performance liquid chromatography wa
81 tion with solid phase extraction followed by reversed phase high performance liquid chromatography.
83 gated for their phenolic profile by means of reversed phase high-performance liquid chromatography co
85 planar chromatography, using water-wettable reversed phase high-performance thin-layer chromatograph
87 cids in sour cassava starch wastewater using reversed-phase high performance liquid chromatography (H
88 ere separated and quantified by an isocratic reversed-phase high performance liquid chromatography (H
90 ention times of N-glycopeptides separated by reversed-phase high performance liquid chromatography (R
91 ermination of vitamin E, being comparable to reversed-phase high performance liquid chromatography ch
92 pic labeling followed by analysis via online reversed-phase high performance liquid chromatography co
94 broadly be categorised into normal phase or reversed-phase high performance liquid chromatography.
95 d against immunoaffinity column (IAC) tandem reversed-phase high pressure liquid chromatography with
96 affinity column (IAC), and identification by reversed-phase high pressure liquid chromatography with
99 tudy, we present the development of coupling reversed-phase high-performance liquid chromatography (H
101 These subunits were separated by ion-pair reversed-phase high-performance liquid chromatography (I
102 rometry (ICPMS), coupled to nano ion pairing reversed-phase high-performance liquid chromatography (n
103 rapid determination of phenolic compounds by reversed-phase high-performance liquid chromatography (R
104 iltration, gel filtration chromatography and reversed-phase high-performance liquid chromatography (R
105 se peptide chemistry and characterized using reversed-phase high-performance liquid chromatography an
107 Quantitation of protein concentrations by reversed-phase high-performance liquid chromatography pr
110 y solid-phase peptide synthesis, purified by reversed-phase high-performance liquid chromatography, a
111 tions of the Osborne fractions determined by reversed-phase high-performance liquid chromatography, s
112 imple method was developed using ion-pairing reversed-phase high-performance liquid chromatography-el
113 culating phylloquinone was measured by using reversed-phase high-performance liquid chromatography.
114 ially available labeling kit and isolated by reversed-phase high-performance liquid chromatography.
116 ts of crude Ou-gon extract were separated by reversed-phase high-performance liquid chromatography.
117 mass spectrometry (MS), in combination with reversed-phase high-pressure liquid chromatography (HPLC
119 pounds of non-V. vinifera grapes, using both reversed phase-high performance liquid chromatography (R
120 protein fractions generated were analyzed by reversed phase-high performance liquid chromatography an
121 ed to identify and quantify the saponins and reversed phase-high performance liquid chromatography co
122 ex, and its subsequent detection by Ion-Pair-Reversed Phase-High Performance Liquid Chromatography-Di
123 ased on enzymatic extraction with subsequent reversed-phase-high-pressure liquid chromatography (RP-H
125 and the sterols are identified/quantified by reversed phase HPLC coupled to tandem mass spectrometry
126 AGE modification sites in plasma proteins by reversed phase HPLC mass spectrometry in tryptic plasma
128 s) in plant extracts was developed, based on reversed phase HPLC separation of extract components, fo
129 ylcobalamin (OH-Cbl), were well separated by reversed phase HPLC with a C8-HPLC column as the station
133 nal NMR spectroscopy, mass spectrometry, and reversed-phase HPLC (log k(w)) and in one case by X-ray
134 s with mAb MF6 and subsequent analysis by C8 reversed-phase HPLC and MS/MS spectrometry and (ii) anal
135 The treated samples were characterized using reversed-phase HPLC and size-exclusion HPLC with absorpt
139 ze exclusion chromatography for homogeneity, reversed-phase HPLC for purity (99%), peptide digest LC-
141 ans- and cis-beta-Carotenes were analyzed by reversed-phase HPLC method using a mobile phase containi
143 purification by HPLC, enzymatic hydrolysis, reversed-phase HPLC resolution of the ribonucleosides, a
144 (BioLCCC) describes polypeptide retention in reversed-phase HPLC using the basic principles of statis
146 lmethoxycarbonyl chemistry, characterized by reversed-phase HPLC, and matrix-assisted laser desorptio
147 hioarsenolipids showed much sharper peaks on reversed-phase HPLC, which facilitated their resolution
149 oked olives were monitored by HPLC/MS-MS and reversed-phase-HPLC methods along different procedures:
151 igosaccharides, separated by size exclusion, reversed phase ion-pairing chromatography, and chip-base
154 We have analyzed GAGs in C. elegans using reversed-phase ion-pairing HPLC, mass spectrometry and i
155 present the development and application of a reversed-phase lauryl methacrylate-based monolith, forme
156 metabolites can be efficiently separated by reversed phase LC and ionized by electrospray ionization
158 e the elution strength of aqueous eluents in reversed phase LC is the application of high temperature
159 luate its performance, we analyzed data from reversed phase LC-MS and hydrophilic interaction chromat
160 0 to 100% MeOH and analyzed with untargeted reversed phase LC-MS showed that the highest number of m
161 by the combination of online two-dimensional reversed-phase LC (2D-LC) operated in high and low pH bu
162 ork, we coupled strong cation exchange (SCX)-reversed-phase LC (RPLC) to CZE-MS/MS for large-scale ph
163 romatographic retention on the commonly used reversed-phase LC columns and the resulting severe ioniz
164 poor chromatographic retention properties in reversed-phase LC, the complex biological matrices, and
169 modifiers for the online coupling of high pH reversed phase liquid chromatography (HPH-RPLC) in the f
170 5 kDa proteolytic fragments were analyzed by reversed phase liquid chromatography (LC) coupled online
171 he first dimension ((1)D) followed by low pH reversed phase liquid chromatography (LPH-RPLC) in the s
176 hic separation techniques, such as nanoscale reversed phase liquid chromatography and capillary elect
177 ed batch experiments with size-exclusion and reversed phase liquid chromatography and in situ infrare
178 nitines was assessed by off-line coupling to reversed phase liquid chromatography coupled to time-of-
180 are compared to those derived by denaturing reversed phase liquid chromatography using an oa-ToF MS
181 rvone, is performed by using on-line coupled reversed phase liquid chromatography with gas chromatogr
183 up level of analysis, its complementarity to reversed phase liquid chromatography, and its hyphenatio
184 utilizing differential isotope labeling and reversed phase liquid chromatography-tandem mass spectro
185 The following isocratic high-performance reversed-phase liquid chromatographic conditions were so
187 profile is commonly performed by ion-pairing reversed-phase liquid chromatography (IPRP) with a mobil
188 italizes on multidimensional high-resolution reversed-phase liquid chromatography (LC) separation for
189 s indicated that incorporation of m-NBA into reversed-phase liquid chromatography (LC) solvents impro
191 capillary zone electrophoresis (CZE)-MS and reversed-phase liquid chromatography (LC)-MS, and then f
192 ne method combining size-exclusion (SEC) and reversed-phase liquid chromatography (RP-HPLC) using a n
193 ophilic interaction chromatography (HILIC) x reversed-phase liquid chromatography (RP-LC) separation
194 hilic interaction chromatography (HILIC) and reversed-phase liquid chromatography (RP-LC) were employ
195 ated the utility of SERS in conjunction with reversed-phase liquid chromatography (RP-LC), for the de
196 erformed using various custom-made prototype reversed-phase liquid chromatography (RPLC) columns rang
197 adequate removal of the stationary phase of reversed-phase liquid chromatography (RPLC) columns.
199 The fractions were separated further by reversed-phase liquid chromatography (RPLC) coupled with
200 ds mostly rely on gas chromatography (GC) or reversed-phase liquid chromatography (RPLC) coupled with
201 ne digestion, followed by a ((2)D) on-column reversed-phase liquid chromatography (RPLC) for reductio
205 Despite recent technological advances in reversed-phase liquid chromatography (RPLC)-mass spectro
206 f ECM proteins, and rapid digestion prior to reversed-phase liquid chromatography (RPLC)-MS analysis.
208 th high confidence and high throughput using reversed-phase liquid chromatography (RPLC)-tandem mass
211 nteraction liquid chromatography (HILIC) and reversed-phase liquid chromatography (RPLC)] together wi
212 pipeline that combines superficially porous reversed-phase liquid chromatography (SPLC), Fourier tra
213 d/l-peptide epimers were separated by online reversed-phase liquid chromatography and fragmented by c
214 ties increased by up to 100-fold compared to reversed-phase liquid chromatography and hydrophilic int
216 r multilinear double pH/solvent-gradients in reversed-phase liquid chromatography are developed by di
217 rs Nochowski from 2012 and 2013 season using reversed-phase liquid chromatography combined with negat
218 een light/heavy pairs under various gradient reversed-phase liquid chromatography conditions, major c
219 pe stripping of human skin, were analyzed by reversed-phase liquid chromatography coupled to high-res
223 -cut multidimensional strong-cation-exchange-reversed-phase liquid chromatography proteomics analysis
224 ple cleanup in a 96-well-plate format before reversed-phase liquid chromatography tandem mass spectro
225 latform encompassing separation via ion-pair reversed-phase liquid chromatography using monolithic st
226 lkaline hydrolysis (1 h at 37 degrees C) and reversed-phase liquid chromatography with negative elect
227 ituted in 25muL acetonitrile and analyzed by reversed-phase liquid chromatography with tandem mass sp
229 nd development of these materials for use in reversed-phase liquid chromatography, wide adoption cont
230 protein groups were identified in single-run reversed-phase liquid chromatography-electrospray ioniza
232 vity in the separation of intact proteins by reversed-phase liquid chromatography-mass spectrometry (
233 olecule is not attainable using conventional reversed-phase liquid chromatography-mass spectrometry m
234 ics and amine metabolomics analyses via nano reversed-phase liquid chromatography-tandem mass spectro
235 ILIC-MS/MS) for polar pesticides, and; (iii) reversed-phase liquid chromatography-tandem mass spectro
240 separation of longer peptides, combined with reversed phase-liquid chromatography (RP-LC) using colum
241 flight MS, and hydrophilic interaction- and reversed phase-liquid chromatography-quadrupole time-of-
243 selectivity compared to conventional bonded reversed-phase materials, along with good peak shape and
246 lid-phase extraction columns consisting of a reversed phase, mixed-mode anion exchange, and mixed-mod
250 -affinity flow configuration hyphenated with reversed phase nanoflow chromatography and coupled with
251 olumn in the first dimension for enrichment, reversed phase nanoLC column in the second dimension for
253 iter scale, by using a single octanol-filled reversed-phase, octadecylsilane-modified (C18-silica) ch
254 00 cm columns fabricated with 5 mum diameter reversed phase particles and integrated electrospray emi
255 d into their corresponding enantiomers under reversed-phase, polar organic and normal-phase condition
257 mode column that has both anion-exchange and reversed-phase properties was used in the first dimensio
258 rotein-level alterations, typically by using reversed-phase protein arrays or mass spectrometry, has
261 using an orthogonal strategy in which both a reversed phase (RP) C18 column and a zwitterionic hydrop
265 syl labeled metabolites can be captured on a reversed phase (RP) trap column for large volume injecti
267 ssed three liquid chromatographic platforms: reversed phase (RP), aqueous normal phase (ANP), and hyd
268 We combined NMR spectroscopy, preparative reversed-phase (RP) chromatography, atomic force microsc
269 ter-soluble metabolites depending on whether reversed-phase (RP) or hydrophilic interaction liquid ch
270 nteraction liquid chromatography (HILIC) and reversed-phase (RP) separation allows a target analysis
271 tide separation modes applied in proteomics: reversed-phase (RP) separations with different pH, hydro
272 ides and components via cation-exchange (CX) reversed-phase (RP) SPE with strategically regulated pH
273 hilic interaction chromatography (HILIC) and reversed-phase (RPLC) chromatography within one analytic
274 ration with 2 retention time segments, while reversed-phase separation was accomplished within 5.5 mi
278 nalytes are preconcentrated onto a dedicated reversed-phase solid-phase extraction (Oasis PRIME-HLB)
279 s, samples (20 mL) were concentrated using a reversed-phase solid-phase extraction (SPE) cartridge, f
280 cross-links as nucleosides, enrichment by a reversed-phase solid-phase extraction column, and nanoLC
282 from analyzed samples by means of polymeric reversed phase Strata X solid phase extraction (SPE) car
283 hanol/water 80:20 (v/v), and cleaned up by a reversed phase/strong cation exchange solid phase extrac
284 extracted sample was chromatographed using a reversed phase system involving an Atlantis T3-C18 colum
288 g power (0.375nm) was further purified using reversed-phase UFLC and subjected to matrix assisted las
289 lyzed applying complementary HILIC and C(18) reversed-phase UHPLC-MS untargeted metabolomic assays.
290 PE) step, and the analytes were separated by reversed-phase ultra high performance liquid chromatogra
291 analysis (EDA) was carried out by combining reversed-phase ultra performance liquid chromatography f
292 of biliverdin were subsequently annotated by reversed-phase ultra-high performance liquid chromatogra
294 ethanesulfonate, (3) sequential ion-exchange/reversed-phase (ultra) high-performance liquid chromatog
295 these less commonly described conjugates by reversed-phase ultrahigh performance liquid chromatograp
296 re trapped online and then analyzed using an reversed-phase ultrahigh-performance liquid chromatograp
298 approach was based on scaling a conventional reversed-phase UPLC-MS method for urinary profiling from
300 trimodal phase incorporating polar embedded reversed phase, weak anion exchange, and strong cation e