ライフサイエンスコーパス: revertant

1 ting gene expression compared to that in the revertant.

2 toms compared to the wild type or a Us3 null revertant.

3 and NR4A3) were down-regulated in the HeLaHF revertant.

4 us progeny virions than the wild type or the revertant.

5 pared to either the parental virus or clonal revertant.

6 s and also for the presence of neurovirulent revertants.

7 tion results in a severe reduction of Lac(+) revertants.

8 nd enforcing cooperative behavior by killing revertants.

9 5 revertant in a subset of persons with T215 revertants.

10 netically unstable, giving rise to wild-type revertants.

11 ation of the viral DNA in the resulting flat revertants.

12 s exploited to isolate dsbA-independent ccdA revertants.

13 ave characterized two classes of ilvA pseudo-revertants.

14 to replicate productively, and can become WT revertants.

15 RNase mutants in vitro can also generate WT revertants.

16 use models including 3H9, 3H9/56R, and their revertant 3H9GL.

17 providing ORF45 in trans or in an ORF45-null revertant (45STOP.R) virus.

18 of mantled, while restoration in spontaneous revertants accounts for non-Mendelian inheritance.

19 with IPV did not increase the proportion of revertants after OPV administration.

20 s occurs while the cells retain a rod shape, revertant alleles with second-site suppressor mutations

21 sorghum show that, compared to functionally revertant alleles, loss of y1 lines do not accumulate ph

22 The revertant also regained virulence and caused significant

23 sing a combination of structure-function and revertant analyses.

24 In addition, a revertant analysis on mmd1 plants demonstrated that Ds-m

25 anscription factor by positional cloning and revertant analysis.

26 Among the 22 revertant and 29 control samples, UDPS detected a mean o

27 Analysis of MPN142 in a cytadhering revertant and complementation using a recombinant wild-t

28 angement of helices supported by second-site revertant and crosslinking analyses, these residues clus

29 eal-time PCR assay to detect and distinguish revertant and nonrevertant OPV serotype 1 (OPV-1), OPV-2

30 compensatory mutations only in UL96 in this revertant and the specific involvement of functionally d

31 for exon 44 or 45 had an elevated number of revertant and trace dystrophin expression (approximately

32 Analysis of genetic revertants and complementation with wild-type alleles co

33 vent this problem, we performed a screen for revertants and dominant suppressors of the bicaudal phen

34 we constructed similar tryptophan auxotroph revertants and found that the reversion resulted from a

35 hrew infection models relying on CSHBV/MSHBV revertants and human HBV will allow comparative assessme

36 cy with which it excises to produce germinal revertants and in its copy number in the maize genome: J

37 more rapidly advantageous than intermediate revertants and may alter the genetic background to rende

38 L420A revertants and pseudorevertants (L420V and L420I) restor

39 aining pqsD, pqsE, and phnAB occurs in these revertants, and quantitative real-time PCR experiments s

40 We demonstrate that wild-type PyMT revertants are derived from mutations in the hotspot seq

41 e as its sole carbon and energy source, Lac+ revertants arise at a constant rate, a phenomenon known

42 stem in sorghum, we have isolated functional revertants as well as loss-of-function alleles of y1.

43 ial oil displayed a significant reduction of revertants at 0.05 mg/plate, from 21% to 34%.

44 the potential to further produce epithelial revertants autonomously.

45 we also repaired the mutation and obtained a revertant, BAC16-45A66F.

46 titution of BRCA2-deficient cells with these revertant BRCA2 alleles rescued PARP inhibitor sensitivi

47 thymidine analogue mutations (TAMs) and T215 revertants (but not T215F/Y) were found to be highly sta

48 We find that Lac+ revertants can completely account for the increase in be

49 Sequence analysis revealed that these revertants carried mutations in dsbB and that their Ps(+

50 ed from Jackfruit pulp reduced the number of revertants caused by aflatoxin B1 (AFB1) and proliferati

51 We hypothesize that these somatic revertant CD18(+) cytotoxic T lymphocytes (CTLs) may hav

52 Over time the revertant CD45RA(pos) effector cell population is also e

53 Importantly, revertant CD8(+) SAP(+) T cells, but not SAP(-) cells, p

54 tudy, Cd(2+)-resistant MT(-/-) (CdR) and CdR revertant (CdR-rev) cell lines were developed and charac

55 a moderate clinical phenotype, and developed revertant cells after the age of 14 years.

56 stible supply of functional patient-specific revertant cells can be obtained--potentially relevant to

57 keratinocyte proliferation, we predict that revertant cells have a selective advantage that allows t

58 When molecularly characterized, revertant cells have rarely exhibited more than one reve

59 ells, and reverted to low methylation in the revertant cells.

60 more slowly for dysfunctional mutants where revertant clones arose.

61 e in humans, is associated with thousands of revertant clones of normal skin that arise from loss of

62 e previously characterized a system in which revertant colonies accumulate slowly and contain cells w

63 ction-only model, the delay in appearance of revertant colonies reflects (1) the time required for in

64 nt chromosomal lac operon gives rise to Lac+ revertant colonies that accumulate over 6 days under sel

65 ed on growth-restricting medium give rise to revertant colonies that accumulate over several days.

66 se parameters are used in a graphic model of revertant colony development, they demonstrate that no i

67 Single-site revertants constructed at these positions suggest that g

68 esidues at E1 position 188 and a second-site revertant containing an E1 K176T mutation.

69 at T cell line led to the emergence of viral revertants containing compensatory mutations not in Gag

70 t RNAP increases several-fold the percent of revertants containing MCMs.

71 amples from untreated persons that lack such revertants ("control" samples).

72 The analysis of the mutants and the revertants demonstrates the importance of a pocket in th

73 trong (Kd 15.2-559 nM), none of the germline revertants displayed any detectable binding to AQP4, rev

74 This mutant, but not its revertant, displays BPES-like conditions such as midface

75 RNA viruses may lead to the accumulation of revertants during manufacture of live viral vaccines, re

76 on in the lac operon (lac-) accumulates Lac+ revertants during prolonged exposure to selective growth

77 Revertant dystrophin fibers, which expressed functional,

78 a growing culture, consistent with Lac+ DNA revertant events.

79 onse regulator gene, while M7 is a wild-type revertant for etaR.

80 Like transferred nTreg and in contrast with revertant Foxp3 cells, Foxp3 iTreg retained CD25 and glu

81 Like transferred nTreg and in contrast with revertant Foxp3- cells, Foxp3+ iTreg retained CD25 and g

82 e highly proliferative tumor cells and their revertants from highly invasive tumor cell populations,

83 The concept of revertant gene mosaicism is also discussed as a potentia

84 c in vivo amelioration of genodermatoses via revertant gene mosaicism will be discussed as a possible

85 ectodermal dysplasias, and the phenomenon of revertant gene mosaicism.

86 eversion frequency and molecular analysis of revertant gene sequences.

87 In contrast, the revertant genome exhibited only a 5-fold reduction in re

88 observations from the full-length mutant and revertant genomes.

89 nt cells have rarely exhibited more than one revertant genotype per patient.

90 titutions resulting in at least 34 different revertant genotypes that restored expression of WASp.

91 A large fraction of these revertant genotypes were also identified in primary T ce

92 Even less obvious, revertant growth may only weakly interfere with vaccine

93 of manufacture, the dynamics of vaccine and revertant growth, plus innate and adaptive immunity elic

94 A ferret-adapted revertant (HA1-H17Y/HA2-R106K) regained airborne transmi

95 The first class of ilvA pseudo-revertants had a mutation in the Phom promoter (P*hom ),

96 By contrast, both revertants had marked effects on G551D-CFTR channel gati

97 All non-wild-type revertants had mutations at T286 and showed defects in b

98 Independent revertants had reproducible expression networks, largely

99 hat observed for genes mutated in PIDs where revertants have not been identified or control genes.

100 It has been reported that ilvA pseudo-revertants having a derepressed hom-thrCB operon appear

101 replication relative to either wild-type or revertant HCMV.

102 The impact of the revertant hematopoietic stem or progenitor cells, partic

103 HA-positive revertant II-3R producing an altered P30 was unexpectedl

104 These revertants implicated a network of interactions that con

105 s or from the primary transmission of a T215 revertant in a subset of persons with T215 revertants.

106 cytomegalovirus (HCMV) mutants harboring the revertant in UL30 (W781V) and UL54 (W780V) DNA polymeras

107 cytomegalovirus (HCMV) mutants harboring the revertant in UL30 (W781V) and UL54 (W780V) DNA polymeras

108 roportion (RP), defined as the percentage of revertants in a sample, differed by < or =10% in 21/25 (

109 , both mutants were more attenuated than the revertants in intranasal and intraperitoneal mouse model

110 sence of wide arrays of individual genotypic revertants in WAS patients and offer opportunities for f

111 mlo-11 resistance and recovered susceptible revertants in which restoration of Mlo function was acco

112 Forty-four were first-site revertants in which the original mutation was changed ba

113 These revertants included pseudorevertants containing acidic o

114 Finally, analysis of nonsense mutation revertants indicates that Polzeta can simultaneously int

115 enhanced virus replication in vivo, and the revertants induced higher-level serum and mucosal antibo

116 However, after considerable delay lpg2(-)REV revertant-infected mice exhibited lesions, and amastigot

117 e significantly higher than in studies using revertant insects.

118 at a second locus during selection for Lac+ revertants is also independent of the proximity of the l

119 The occurrence of revertants is considered rare, and the underlying geneti

120 utations but that the nature of the selected revertants is influenced by both the viral background an

121 lix in wild-type TpoR and in the second-site revertants is likely associated with its strong preferen

122 Using a set of parental, gene-disrupted, and revertant isogenic clones, we found that RESA plays a ma

123 Transcriptome analysis of prion-resistant revertants, isolated from highly susceptible cells, reve

124 dons within the K1 ORF (KSHV-K15xSTOP), or a revertant K1 virus (KSHV-K1REV).

125 gnificantly lower level than its BAC-derived revertant (KSHVdLZRev) or KSHVWT (BAC36) in HEK 293T cel

126 Revertants (L420) or pseudorevertants (L420V and L420I)

127 zed by the development of white, genetically revertant macules in red, diseased skin.

128 In one class of revertants, Miranda still binds Staufen/oskar mRNA compl

129 Revertant mosaicism (RM) is a naturally occurring phenom

130 This case represents a novel mechanism of revertant mosaicism and is an example of "natural gene t

131 episodes in infancy, harbored hematopoietic revertant mosaicism by uniparental disomy of 7q, with lo

132 o MDS with -7/del(7q), whereas hematopoietic revertant mosaicism commonly ameliorated clinical manife

133 rameshift mutations in the high frequency of revertant mosaicism in IWC.

134 Here we describe a disseminated pattern of revertant mosaicism observed in 6 patients with Kindler

135 Clinically, revertant mosaicism was associated with milder disease,

136 cell therapy, innovations in cancer biology, revertant mosaicism, and induced pluripotent stem cell t

137 Spontaneous gene repair, also called revertant mosaicism, has been documented in several gene

138 lopment of hundreds of normal skin spots via revertant mosaicism.

139 on of low-grade constitutional, somatic, and revertant mosaicism; and provided evidence of a mutation

140 Unexpectedly, the myristyl group of a revertant mutant with normal PM targeting properties (V7

141 n a deletion veA (DeltaveA) strain to obtain revertant mutants (RM) that regained the capability to p

142 agenesis in a DeltaveA strain and identified revertant mutants able to synthesize ST, among them RM1.

143 and molecular characterization of one of the revertant mutants, RM3, revealed that a point mutation o

144 rature, CFTR-targeting drugs and second-site revertant mutation R1070W.

145 in vivo in a WAS patient with a spontaneous revertant mutation, indicating that altered Treg fitness

146 7% harboring T215Y/F and 2.7% harboring T215 revertant mutations (T215rev).

147 Here, we investigate the effects of the revertant mutations G550E and 4RK (the simultaneous disr

148 Thus, the revertant mutations G550E and 4RK alter the gating patte

149 To learn how revertant mutations influence G551D-CFTR function, we st

150 3(GPC/VGKS) by introducing the corresponding revertant mutations K465V and G467K within GP2 of rCl-13

151 is ATP independent, we investigated whether revertant mutations restore ATP dependence to G551D-CFTR

152 T215 revertant mutations such as T215C/D/E/S that evolve from

153 mpound heterozygous mice invariably acquired revertant mutations that restored cysteine 181.

154 ion; and (c) introduction of solubilizing or revertant mutations to stabilize F508del NBD1 reduced it

155 efects in CFTR processing and function using revertant mutations.

156 The enrichment of WASP+-revertant NK cells indicates that WASP provides a select

157 SP), and NK cells contained both mutated and revertant (normal) sequences.

158 A spontaneous drug-resistant revertant of BamA(DeltaR44) was found to carry an A18S s

159 study, we isolated dLeuR, a growth-competent revertant of dLeu.

160 A partial revertant of H358-G200 cells had reduced levels of RRM1

161 ale was brought up by the observation of the revertant of SCIDX1 and ADA deficiency as a kind of natu

162 Furthermore, in vivo a Gal(+) revertant of this mutant outcompeted the galETKM deletio

163 Revertants of a colcemid-resistant Chinese hamster ovary

164 In the first study reported here, revertants of a set of cosQ mutants were screened for su

165 This was found through analysis of revertants of a severely defective mutant of murine hepa

166 Sequence analysis of 40 degrees C revertants of Alb/ts/nsp5/V148A identified primary rever

167 re PCR positive contained varying amounts of revertants of all 3 poliovirus serotypes.

168 -of-function alleles of egl-30 as intragenic revertants of an egl-30 reduction-of-function mutation.

169 Analysis of multiple second-site revertants of CCA4 revealed mutations in both the M prot

170 HeLaHF cells are transformation revertants of cervical cancer HeLa cells and have lost a

171 e this chromosome loss phenotype, intragenic revertants of fla8-1, fla8-2, and fla10-14 were generate

172 In contrast, spontaneous motile revertants of fliL cells that regained motility yet prod

173 Partial framework revertants of HIV-1 broadly neutralizing Abs may present

174 Finally, the analysis of the second-site revertants of K377C reveals that mutation of Ile-22 (in

175 Analysis of second-site revertants of MDelta2 revealed mutations in the carboxy-

176 The revertants of shortened H protein mutants fell into two

177 Revertants of single-amino-acid substitution mutants wer

178 Motile (Mot+) revertants of the -38C:T mutant were isolated and charac

179 stigate this budding defect, we selected for revertants of the E2-H348/352A double mutant.

180 t the findings of phenotypic (not genotypic) revertants of the ext3 mutant and in-depth analysis incl

181 140A) was isolated from antibiotic-resistant revertants of the hypersensitive TolC(R367H) mutant.

182 ow isolated multiple independent second-site revertants of the loop 1 insertion mutant, and we used r

183 Mapping of revertants of the resulting chimeric viruses provided ev

184 involves monitoring the appearance of Lac(+) revertants of the strain FC40 under starvation condition

185 By selecting revertants of the temperature sensitive and paclitaxel h

186 iochemical and molecular analyses of several revertants of the w4-m allele, we have shown that the W4

187 Revertant OPV-1 and nonrevertant OPV-2 and -3 were detec

188 Revertant OPV-1 was found in stool at 7 and 9 weeks, and

189 215Y or T215F was not detected in any of the revertant or control samples; however, 4 of 22 revertant

190 taining mutants; rather, only true wild-type revertants or a virus, G50U/C47A, containing a second si

191 nsmitted T215Y variants by the more fit T215 revertants or from the primary transmission of a T215 re

192 relative to two independently produced vLIP revertants or parental virus.

193 Additionally, in some revertant patches, mitotic recombination generated areas

194 ) and HCAs, measured as the log of histidine revertants per nanomole of amine, log m, in Salmonella t

195 s direct mutagens (10(-7) mol/plate), with a revertants percentage reduction of 39% and 40%, respecti

196 terizing the molecular lesions that confer a revertant phenotype.

197 ndividual vaccinees, it can acquire specific revertant point mutations, leading to vaccine-associated

198 nd a second antibiotic suppressing the small revertant population may be superior to alternatives suc

199 Here we identified a partial revertant population of the L. major lpg2- mutants (desi

200 In vivo-isolated motile revertants possessed an identical, single extragenic mut

201 ed the amounts of adaptive his, met, and leu revertants produced by the B. subtilis YB955 parental st

202 These revertants produced more pyocyanin and had increased lev

203 When reversion was compared, the revertant proportion (RP), defined as the percentage of

204 We show that revertant RDEB keratinocytes expressing functional C7 ca

205 U residues were identified at the 3' ends of revertants recovered from Huh7 cells transfected with an

206 able microtubule levels, and virus-sensitive revertants recovered from the mutant line showed restora

207 Interestingly, rNS3-5B-L726P (revertant) replicated with the same efficiency as the rN

208 Spontaneous revertants restored for flagellar biosynthesis, gene exp

209 Together the revertants reveal specific and interconnected aspects of

210 he FeoB2-deficient mutant, and the same-site revertant revealed that the mutant had a significantly d

211 ion by extensive mutagenesis and analyses of revertants revealed that two consecutive C residues (C(9

212 les from untreated persons that contain T215 revertants ("revertant" samples) compared with samples f

213 , and compared it with that of a spontaneous revertant (RF111).

214 eceptor with relatively high efficiency, the revertant RGD viruses utilized either the alpha(V)beta(1

215 cularly infected mice compared to the NgK or revertant (RgK) virus.

216 Characterization of the mutant and revertant RNA molecules and the corresponding viruses co

217 In 6 of 22 (27%) revertant samples and in 4 of 29 control samples (14%; P

218 vertant or control samples; however, 4 of 22 revertant samples had one or more T215 revertants that w

219 ure to detect viruses with T215Y/F in the 22 revertant samples in this study may result from the over

220 eated persons that contain T215 revertants ("revertant" samples) compared with samples from untreated

221 By selecting revertants, second-site mutations were identified for on

222 Further investigation revealed that a revertant selected for growth on pyruvate regained the i

223 d sewage and to distinguish nonrevertant and revertant serotypes and demonstrated that OPV continues

224 ses of the fliL parent strain and its motile revertants showed that they result from mutations alteri

225 the reversion mechanism in all investigated revertant skin spots.

226 Analysis of revertant SL-1 mutant viruses revealed that a compensato

227 on in ichthyosis with confetti suggests that revertant stem cell clones are under strong positive sel

228 binant ORF134-deleted strain and the derived revertant strain suggested that cyprinid herpesvirus 3 I

229 th either the parental or the reconstituted (revertant) strain.

230 hyde, than biofilms formed by the parent and revertant strains (P < 0.0001), demonstrating that the e

231 our patient the functional advantage of the revertant T cells in the context of persistent CMV infec

232 l patients from the same family who also had revertant T lymphocytes that showed selective in vivo ad

233 rienced progressive clinical improvement and revertant T-cell mosaicism.

234 sel branches are few and upright in the wab1 revertant tassel and have an increased branch angle in t

235 No fibril was formed by a revertant that exhibits the stable wild-type GB1 fold or

236 s RNAs that produced some plaques from which revertants that always restored the Arf activation prope

237 hat appear during lactose selection are true revertants that arise in a single step from Lac- cells,

238 Thus vaccine revertants that delete or inactivate the transgene may e

239 ral independent, genetically stable germline revertants that lacked the duplicated wild-type sequence

240 Here, we have isolated and characterized revertants that rescued the fusion and growth defects of

241 the left end, and was rapidly supplanted by revertants that restored asymmetry.

242 rees C cold-sensitive phenotype and selected revertants that restored vector productivity.

243 of 22 revertant samples had one or more T215 revertants that were detected by UDPS but not by direct

244 ro Unlike wild-type virus or a gH-containing revertant, the gH-null mutant was unable to infect any o

245 In the second class of ilvA pseudo-revertants, the thrR gene encoding a putative DNA-bindin

246 in the UL15 gene restored the ability of the revertant to cleave and package viral DNA.

247 Swiss mice than that of wild-type virus, and revertants to the wild type could be detected by PCR clo

248 es symport; how some multiple mutants become revertant transporters; the raised export rate and affin

249 number and delayed appearance of two of the revertant types.

250 to identify genome differences among vaccine revertants, vaccine strains, and field isolates, whole-g

251 Here, we show that hepatotropic revertant variants may be selected from these in vitro i

252 S3-negative MDV was completely restored in a revertant virus (20US3*) expressing a US3 protein with a

253 racteristics similar to those of SPPV-SA and revertant virus (RvKLP).

254 nization with IPV and OPV has on shedding of revertant virus by healthy infants.

255 ction into human fibroblast cells, whereas a revertant virus readily produced viral plaques and, subs

256 ing the immune response to this virus with a revertant virus that can persist, we were able to dissec

257 A revertant virus that restored the expression of the meq

258 Importantly, the revertant virus that restored the expression of vIL-8 ge

259 P, RRV-GFP, wild-type (WT) RRV H26-95, and a revertant virus using traditional plaque assays, as well

260 The gK-V5-TEV, R-gK-V5-TEV (revertant virus), and gDDeltaTEV viruses exhibited simil

261 shuffled RTA constructs did not yield any WT revertant virus, a sharp contrast to WT virus contaminat

262 of DNA cleavage were comparable to those for revertant virus, deletion of M140 resulted in a signific

263 electron microscopy showed that, compared to revertant virus, the number of double-membrane vesicles

264 ine (IPV) resulted in faster accumulation of revertant virus, thus potentially increasing the risk of

265 plaque size is reduced compared to that of a revertant virus.

266 operties compared to RRV-GFP, WT RRV, or the revertant virus.

267 tic growth defect that was not observed in a revertant virus.

268 th either parental BAC20 virus or the 20US3* revertant virus.

269 the appearance of fluorescence indicating a revertant virus.

270 Phenotypically revertant viruses arose after continued passage in cultu

271 P112A affected virion assembly, we selected revertant viruses for these two mutants.

272 ansmission of ChimeriVax vaccine recombinant/revertant viruses in nature is minimal.

273 Despite the presence of virulent revertant viruses in some live-attenuated vaccines, dise

274 ns and illustrate the expanding diversity of revertant viruses in this population.

275 A3 genes has been independently deleted, and revertant viruses in which the genes have been re-introd

276 Replication-competent, revertant viruses rescued from dicistronic HRV-14 RNAs c

277 Revertant viruses that formed plaques on Vero cells were

278 Nonetheless, revertant viruses that restored the WT CA sequence and h

279 Four viable second-site revertant viruses were isolated from three different rep

280 ailed to rescue rPIV5-P-T286E virus, several revertant viruses were obtained.

281 was reduced compared to that of wild-type or revertant viruses when the expression of only a single g

282 not support viral RNA replication, and only revertant viruses with a restored wild-type arginine or

283 Nevertheless, unlike parental and revertant viruses, the mutants induce moderate levels of

284 or differences in egress for the mutant and revertant viruses.

285 and virus shedding compared to parental and revertant viruses.

286 Like the lpg2 parent, the lpg2(-)REV revertant was unable to synthesize LPG2-dependent PGs in

287 A collection of photosynthetic revertants was obtained from Delta26pAtE, and gel blot h

288 ntified by flow cytometry to have 10% to 15% revertant, WAS protein-expressing lymphocytes in his blo

289 However, two (33.3%) germline autoantibody revertants were polyreactive and four (66.7%) were autor

290 We show that engineered intermediate revertants were viable but had no increased replication

291 this system, simple excisions produce purple revertants, whereas deletions of host or transposon sequ

292 s well as cAMP levels, are elevated in these revertants, while Pseudomonas quinolone signal (PQS) pro

293 Revertant wild-type PyMT (containing nine cytosines) was

294 Notably, a germline revertant with mature CDR3s neutralizes 12% of viruses a

295 We identified eleven second-site revertants with improved virus growth and mutations in t

296 tance results in an overwhelming majority of revertants with MCMs.

297 h AID results in a high percentage of Kan(R) revertants with MCMs.

298 The E89 mutants yielded revertants with second-site substitutions within regions

299 A series of partial revertants within the mutated LAGLIDADG region are shown

300 compared with a normal-colony variant (NCV) revertant, yet the SCV presented the hallmarks of S. aur