ライフサイエンスコーパス: ribonuclease A

1 reduced non-specific displacement by BSA and ribonuclease A.

2 thiols from either dithiothreitol or reduced ribonuclease A.

3 es was demonstrated with a tryptic digest of ribonuclease A.

4 -PLC) has been suggested to resemble that of ribonuclease A.

5 to the one observed earlier for valine 63 in ribonuclease A.

6 H dependence of the folding and unfolding of ribonuclease A.

7 e found in the active site of the prototype, ribonuclease A.

8 process analogous to that used by the enzyme ribonuclease A.

9 catalyzed by specific base, Zn(2+) ions, or ribonuclease A.

10 ical exchange is demonstrated on the protein ribonuclease A.

11 1 snRNP that was sensitive to treatment with ribonuclease A.

12 er similar to that of the catalytic triad of ribonuclease A.

13 mpounds were indeed selective for displacing ribonuclease A.

14 mo-trypsinogen A, it had no interaction with ribonuclease A.

15 lid heavy-chain antibody (VHH) that binds to ribonuclease A.

16 d model protein fragment, the C peptide from ribonuclease A.

17 e extensively studied case of reduced bovine ribonuclease A (13,689 Da), only Asn67 deamidation has b

18 Refolding intermediates of bovine pancreatic ribonuclease A, a model system for this study, are block

19 were conducted using the model protein pairs ribonuclease A/alpha-chymotrypsinogen A and cytochrome C

20 nts were conducted with a mixture containing ribonuclease A and alpha-chymo-trypsinogen A which exhib

21 two protein pairs, alpha-chymotrypsinogen A/ribonuclease A and cytochrome c/lysozyme, using batch an

22 the nonspecific binding of bovine pancreatic ribonuclease A and Escherichia coli lac repressor to dou

23 euterium exchange data from disulfide-intact ribonuclease A and from cytochrome c are discussed to ex

24 is of the [M + 12H]12+ to [M + 5H]5+ ions of ribonuclease A and its N-linked glycosylated analogue, r

25 nt reductive unfolding rates and pathways of ribonuclease A and its structural homologue onconase can

26 ers of [PO(3)]((-)), can bind to the enzymes ribonuclease A and NAD kinase, raising the question of w

27 d lower than the maximum values exhibited by ribonuclease A and ONC, respectively, and there is littl

28 the cleavage reaction is similar to that of ribonuclease A and the arrangement of the active sites i

29 zing acetyl-PEO-biotin labeled peptides from ribonuclease A and the ICAT-labeled proteome of Deinococ

30 of the cspA mRNA was used as a substrate for ribonucleases A and T1, the addition of CspA significant

31 omplexes are treated with elevated levels of ribonucleases A and T1, the nascent transcript can be cl

32 cytochrome c, alpha-chymotrypsinogen A, and ribonuclease A) and six acidic proteins (myoglobin, deox

33 tryptic peptide from unfolded and deamidated ribonuclease A, and a tryptic peptide from calmodulin de

34 Ovalbumin, ribonuclease A, and hemoglobin are used as model systems

35 rial immunity protein Im7, bovine pancreatic ribonuclease A, and human anti-HER2 single-chain Fv anti

36 e I region of poly-l-lysine, concanavalin A, ribonuclease A, and lysozyme show cross-peaks between th

37 or four of the proteins (ubiquitin, eglin c, ribonuclease A, and lysozyme) appear to converge to a co

38 h three reduced secreted proteins (lysozyme, ribonuclease A, and riboflavin binding protein (RfBP)).

39 sozyme, bovine pancreatic trypsin inhibitor, ribonuclease A, and T4 lysozyme) were examined carefully

40 or L in the model protein, bovine pancreatic ribonuclease A, and through analysis of temperature fact

41 tulinum toxin, Staphylococcus enterotoxin B, ribonuclease A, and thyroglobulin.

42 sure-assisted cold denaturation of lysozyme, ribonuclease A, and ubiquitin is presented.

43 REACH lattice dynamics model of crystalline ribonuclease A are also in satisfactory agreement with t

44 peratures of both hen egg white lysozyme and ribonuclease A are sensitive to the PA of the PIL as muc

45 apparatus with both stationary phases using ribonuclease A as a model protein and applied potentials

46 Using ribonuclease A as a model protein system, we demonstrate

47 ction time were systematically studied using ribonuclease A as a model protein.

48 dative folding pathways of bovine pancreatic ribonuclease A at pH 8.0 and 25 degrees C involve a pre-

49 ulation study of water hydrating the protein Ribonuclease A, at a series of temperatures in cluster,

50 validated under column conditions, with the ribonuclease A being displaced and the alpha-chymotrypsi

51 ll-characterized proteins (bovine pancreatic ribonuclease A, bovine pancreatic trypsin inhibitor, and

52 netics of disulfide-intact bovine pancreatic ribonuclease A by fluorescence-detected stopped-flow tec

53 tudies of the glycation of the model protein ribonuclease A by glucose and ribose leading to the form

54 sinogen A, while no binding was observed for ribonuclease A, confirming that protein-displacer bindin

55 nthesis that involves the ECERIFERUM7 (CER7) ribonuclease, a core subunit of the exosome.

56 Five proteins (ribonuclease A, cytochrome c, lysozyme, myoglobin, bovin

57 ly(A) polymerase, and PARN [poly(A)-specific ribonuclease], a deadenylase.

58 Myoglobin, lysozyme, beta-lactoglobulin, ribonuclease A, E-cadherin 5, and concanavalin A were co

59 models of ribonuclease cleavage and for the ribonuclease A enzyme itself, based on our studies of th

60 ole of four epididymis-specific noncanonical ribonuclease A family genes in regulating fertility and

61 that bond, analogous to the mechanism of the ribonuclease A family of protein enzymes and heavily mod

62 s subdomains on the rate-determining step in ribonuclease A folding and on the physical structure-for

63 Ribonuclease A had no antiviral activity even at approxi

64 the oxidative refolding of bovine pancreatic ribonuclease A has been characterized.

65 (Uvf) of disulfide-intact bovine pancreatic ribonuclease A has been monitored by circular dichroism

66 A variant of bovine pancreatic ribonuclease A has been prepared with seven amino acid s

67 For nontarget components of the mixture (ribonuclease A, holotransferrin, and apomyoglobin), the

68 nucleoprotein complexes become resistant to ribonuclease A hydrolysis.

69 on model proteins, such as cytochrome c and ribonuclease A, identified a limited number of peptide c

70 e show that rabbit antibodies to MG-modified ribonuclease A identify proteins modified by the Maillar

71 able-pressure NMR data for bovine pancreatic ribonuclease A in 2H2O at pH 2.0 and 295 K yielded the f

72 egation line observed for both ovalbumin and ribonuclease A in ammonium sulfate, interpreted theoreti

73 pon unfolding of model proteins lysozyme and ribonuclease A, in solutions containing varying cosolute

74 state of disulfide-intact bovine pancreatic ribonuclease A is a heterogeneous mixture of unfolded sp

75 state of disulfide-intact bovine pancreatic ribonuclease A is a heterogeneous mixture of unfolded sp

76 We show that the oxidative refolding of ribonuclease A is catalyzed by this system in a quinone-

77 a K(o)T indicates that the denatured form of ribonuclease A is more compressible than the native form

78 ermine if the native-like intermediate IN of ribonuclease A is on or off-pathway.

79 atalysis of RNA 2'-O-transphosphorylation by ribonuclease A is proposed to involve electrostatic stab

80 escence unfolding phase of bovine pancreatic ribonuclease A is studied by stopped-flow kinetics and s

81 olding of disulfide-intact bovine pancreatic ribonuclease A is used as an example to illustrate the k

82 studies of the homologous protein guinea pig ribonuclease A; it is proposed here that Lys113 in the l

83 so, it was also found that ligand binding to ribonuclease A led to changes in alpha, suggesting a bur

84 es, with an ancient evolutionary link to the ribonuclease A-like superfamily.

85 for five model proteins (ubiquitin, eglin c, ribonuclease A, lysozyme, and cytochrome c).

86 threitol (DTT)-dependent reduction of native ribonuclease A, microbial ribonuclease, and pancreatic t

87 olomyoglobin, as well as native lysozyme and ribonuclease A, nevertheless, TFE stabilizes native apom

88 The published data on kinetic effects with ribonuclease A of substituting thiophosphate groups for

89 est (by microinjection with ricin A chain or ribonuclease A) of the inducer or either of the fusion p

90 eight cysteine residues of reduced unfolded ribonuclease A or to site-specific locations using appro

91 Four model proteins (ribonuclease A, ovalbumin, myoglobin, BSA) were separate

92 It has been reported that His-119 of ribonuclease A plays a major role as an imidazolium ion

93 ments of the global dynamics of lysozyme and ribonuclease A powders.

94 nsions of a denatured protein, fully reduced ribonuclease A (r-RNase A), have been measured using syn

95 haracteristics of reduced and carboxamidated ribonuclease A (RCAM RNase) were determined for transfer

96 ats and nonrepeating sequences, form stable, ribonuclease A-resistant structures.

97 n a disulfide-bond-containing loop region of ribonuclease A results in the localized modulation of pr

98 For ribonuclease A, results obtained in both water and deute

99 oxidative folding of both bovine pancreatic ribonuclease A (RNase A) and a 58-72 fragment thereof fr

100 in (BSA) and fibrinogen), including enzymes (ribonuclease A (RNase A) and alkaline phosphatase).

101 uantitative noncovalent interactions between ribonuclease A (RNase A) and cytidylic acid ligands (2'-

102 The interactions between bovine pancreatic ribonuclease A (RNase A) and its RNA substrate extend be

103 The interaction between bovine pancreatic ribonuclease A (RNase A) and its RNA substrate extends b

104 Using ribonuclease A (RNase A) and saporin as two representati

105 o the oxidative folding of bovine pancreatic ribonuclease A (RNase A) and show that des[40-95] and de

106 Ribonuclease A (RNase A) and the ribonuclease inhibitor

107 miting millisecond motions in wild-type (WT) Ribonuclease A (RNase A) are modulated by histidine 48.

108 Ribonuclease A (RNase A) can make multiple contacts with

109 Ribonuclease A (RNase A) catalyzes the cleavage of RNA a

110 Bovine pancreatic ribonuclease A (RNase A) cleaves this substrate with a k

111 es have shown that divalent anion binding to ribonuclease A (RNase A) contributes to RNase A folding

112 The dimer of bovine pancreatic ribonuclease A (RNase A) discovered by Crestfield, Stein

113 It belongs to the bovine pancreatic ribonuclease A (RNase A) family and exhibits ribonucleol

114 inophilic leukocytes that is a member of the ribonuclease A (RNase A) family of ribonucleases.

115 three-disulfide mutant of bovine pancreatic ribonuclease A (RNase A) from the fully reduced unfolded

116 The regeneration of bovine pancreatic ribonuclease A (RNase A) from the reduced to the native

117 During the regeneration of bovine pancreatic ribonuclease A (RNase A) from the reduced to the native

118 Bovine pancreatic ribonuclease A (RNase A) has a conserved His ...

119 idative folding pathway of bovine pancreatic ribonuclease A (RNase A) has been examined at various pH

120 Ribonuclease A (RNase A) has long been known to spontane

121 tes formed during the oxidative refolding of ribonuclease A (RNase A) have been characterized.

122 nd [C65S, C72S] mutants of bovine pancreatic ribonuclease A (RNase A) have been studied.

123 The crystal structure of ribonuclease A (RNase A) in complex with pdUppA-3'-p [5'

124 vated by two different schemes to immobilize ribonuclease A (RNase A) in either a preferred orientati

125 P93A, P114A, and P117A) of bovine pancreatic ribonuclease A (RNase A) in which each mutant has one of

126 Bovine pancreatic ribonuclease A (RNase A) is a 124-residue enzyme that co

127 Bovine pancreatic ribonuclease A (RNase A) is a distributive endoribonucle

128 The active-site cleft of bovine pancreatic ribonuclease A (RNase A) is lined with cationic residues

129 three-disulfide mutants of bovine pancreatic ribonuclease A (RNase A) missing the 65-72 disulfide bon

130 oxidative regeneration of bovine pancreatic ribonuclease A (RNase A) proceeds through des-[40-95] RN

131 Select members of the bovine pancreatic ribonuclease A (RNase A) superfamily are potent cytotoxi

132 The ribonuclease A (RNase A) superfamily has been the subjec

133 in human angiogenin (hANG), a member of the ribonuclease A (RNase A) superfamily known to be involve

134 pread and functionally varied members of the ribonuclease A (RNase A) superfamily provide an excellen

135 an amphibian member of the bovine pancreatic ribonuclease A (RNase A) superfamily, is in phase III cl

136 neurotoxin (EDN), a protein belonging to the ribonuclease A (RNase A) superfamily, which has recently

137 f the polypeptide chain of bovine pancreatic ribonuclease A (RNase A) that are critical for stabilizi

138 Mutants of bovine pancreatic ribonuclease A (RNase A) that contain four of the eight

139 Ribonuclease A (RNase A) undergoes more rapid conformati

140 ar dynamics simulations of bovine pancreatic ribonuclease A (RNase A) up to its melting temperature (

141 The evolution of the ribonuclease A (RNase A) vertebrate-specific enzyme fami

142 3'-phosphate (pTppAp) with bovine pancreatic ribonuclease A (RNase A) was characterized by calorimetr

143 n the dissociation of enzymatic product from ribonuclease A (RNase A) was investigated by creation of

144 Rapid single-turnover kinetics of ribonuclease A (RNase A) was measured with better than m

145 The dynamic properties of the enzyme ribonuclease A (RNase A) were investigated through the u

146 s (space group P3(2)21) of bovine pancreatic ribonuclease A (RNase A) were prepared at a pH of 5.5 in

147 setrade mark, a homolog of bovine pancreatic ribonuclease A (RNase A) with high conformational stabil

148 Onconase, a homolog of ribonuclease A (RNase A) with low ribonucleolytic activi

149 rvested from wild-type mice generated CML on ribonuclease A (RNase A), a model protein, by a pathway

150 was compared with that of the parent enzyme ribonuclease A (RNase A), and a model was devised to ass

151 We now demonstrate that a model protein, ribonuclease A (RNase A), exposed to free L-serine and H

152 its structural homologue, bovine pancreatic ribonuclease A (RNase A), has been isolated and characte

153 enin (Ang), a homologue of bovine pancreatic ribonuclease A (RNase A), is a potent inducer of blood v

154 Onconase (ONC), a homologue of ribonuclease A (RNase A), is in clinical trials for the

155 enin (ANG), a homologue of bovine pancreatic ribonuclease A (RNase A), promotes the growth of new blo

156 In bovine pancreatic ribonuclease A (RNase A), the His...Asp dyad is composed

157 used to analyze salt effects on catalysis by ribonuclease A (RNase A), which is a cationic enzyme tha

158 ONC is a homolog of ribonuclease A (RNase A).

159 re critical for catalysis of RNA cleavage by ribonuclease A (RNase A).

160 ytic mechanism highly reminiscent of that of ribonuclease A (RNase A).

161 iewed and illustrated with bovine pancreatic ribonuclease A (RNase A).

162 sis reactions catalyzed by bovine pancreatic ribonuclease A (RNase A).

163 e regeneration pathways of bovine pancreatic ribonuclease A (RNase A).

164 for inhibition of the enzymatic activity of ribonuclease A (RNase A).

165 , turkey ovomucoid third domain (OMTKY3) and ribonuclease A (RNase A).

166 y the backbone dynamics of bovine pancreatic ribonuclease A (RNase A).

167 nt in reduced and unfolded bovine pancreatic ribonuclease A (RNase A).

168 nant HsQSOX1 is highly active toward reduced ribonuclease A (RNase) and dithiothreitol but shows a >1

169 e (LiP) was examined using bovine pancreatic ribonuclease A (RNase) as a polymeric lignin model subst

170 g an Amadori intermediate in the reaction of ribonuclease A (RNase) with ribose for rapid studies of

171 ffusivity of the positively charged protein, ribonuclease A (RNase), in solutions of dextrans of vari

172 g the refolding of reduced bovine pancreatic ribonuclease A (RNase).

173 e pairings in two unfolded reduced proteins: ribonuclease A (RNase, four disulfide bonds and 105 disu

174 g a protein corona structure consisting of a ribonuclease-A (RNase-A) on the particle surfaces.

175 p-aminobenzamidine (pABA), bovine pancreatic ribonuclease A (RNaseA), and uridine-3'-phosphate (3'UMP

176 rotein model mixture comprised of ubiquitin, ribonuclease A (RNaseA), cyclophilin A (CypA), and bovin

177 r model protein systems including ubiquitin, ribonuclease A (RNaseA), cyclophilin A (CypA), and bovin

178 kinetics, as demonstrated by the binding of ribonuclease A (RNaseA, 13.7 kDa) with cytidine nucleoti

179 panning the N-terminal 20 residues of bovine ribonuclease A (S peptide).

180 Analysis of 1.57 nmol of ribonuclease-A shows high sensitivity in one- and two-di

181 sodium chloride for six proteins (ovalbumin, ribonuclease A, soybean trypsin inhibitor, lysozyme, and

182 glyceraldehyde-3-phosphate dehydrogenase and ribonuclease A, substrates for CMA.

183 eless as effective as 3-MHD in cross-linking ribonuclease A suggested that protein lysine condensatio

184 ibility of the region to ribonuclease V1 and ribonuclease A suggesting the geometry formed by the rep

185 tations in Angiogenin (ANG), a member of the Ribonuclease A superfamily (also known as RNase 5) are k

186 Angiogenin, a member of the ribonuclease A superfamily (RNase A), cleaves tRNAs to g

187 onuclease cluster, a group of eight distinct ribonuclease A superfamily genes that are more closely r

188 Angiogenin (hANG), a member of the Ribonuclease A superfamily has angiogenic, neurotrophic

189 bonucleases (EARs), which are members of the ribonuclease A superfamily with known antipathogen activ

190 tations in angiogenin (ANG), a member of the ribonuclease A superfamily, are associated with amyotrop

191 Onconase (P-30 protein), an enzyme in the ribonuclease A superfamily, exerts cytostatic, cytotoxic

192 n dielectric constant; (4) the pKa shifts in ribonuclease A that result from phosphate binding are re

193 helix formation in the isolated C-peptide in ribonuclease A, there is growing evidence that a signifi

194 Addition of animal-derived ribonuclease A to degrade RNA impurities is not recommen

195 Model proteins were studied: ribonuclease A, trypsin inhibitor, and carbonic anhydras

196 When compared with other amphibian ribonucleases, a typical pattern of cysteine residues is

197 After the recent discovery of a ribonuclease A unfolding intermediate, we investigated t

198 ermally induced unfolding of lysozyme and of ribonuclease A was determined by means of differential s

199 bovine serum albumin, and bovine pancreatic ribonuclease A, we demonstrate that DOPA2 cross-linking

200 somerase IIalpha interaction is sensitive to ribonuclease A, we explored whether the RHA-topoisomeras

201 Previous data on cold-denatured ribonuclease A were reevaluated and compared to known fo

202 glyceraldehyde-3-phosphate dehydrogenase and ribonuclease A when the reaction was supplemented with B

203 This report focuses on regions of ribonuclease A where the rate constant enhancements are

204 w-folding and fast-folding forms of unfolded ribonuclease A, which led to the understanding that prol

205 (Rana pipiens) contain another homologue of ribonuclease A, which we named Amphinase (Amph).

206 lectivity, displacing essentially all of the ribonuclease A while displacing minimal alpha-chymotryps

207 etreatment of glyceraldehyde-3-phosphate and ribonuclease A with BOH increased their rate of degradat

208 n-6 with barium chloride, and the binding of ribonuclease A with cytidine 2'-monophosphate within rea

209 The disulfide-reduced form of bovine ribonuclease A, with the Cys thiols irreversibly blocked

210 Three tyrosine-to-phenylalanine mutants of ribonuclease A (Y25F, Y92F, and Y97F) are investigated f