ライフサイエンスコーパス: sample preparation

1 mode SPE clean-up with different sorbents in sample preparation.

2 tap water, rainwater, and seawater) with no sample preparation.

3 an be visually isolated quickly with minimal sample preparation.

4 ing sugars in potatoes, without the need for sample preparation.

5 d system endurance in combination with minor sample preparation.

6 d blood spot (DBS) samples with virtually no sample preparation.

7 s to investigate embalming materials without sample preparation.

8 pipetting platform was utilized for one-pot sample preparation.

9 itor and report the pH of suspensions during sample preparation.

10 ch as (LA)-ICPMS or coupled to a destructive sample preparation.

11 in a few minutes without additional tailored sample preparation.

12 es rapid, efficient, and highly reproducible sample preparation.

13 (165) concentration in serum without further sample preparation.

14 or SALDI-MS compared well to ultrafiltration sample preparation.

15 r system to conduct measurements without any sample preparation.

16 and digestion benchmarks the quality of the sample preparation.

17 l DNA into a PCR solution using our DM-based sample preparation.

18 alyze multiple cell populations from each EM sample preparation.

19 berglass collection wipes with no additional sample preparation.

20 ysis of biological samples with little or no sample preparation.

21 ntial time and human resources necessary for sample preparation.

22 ds are accessible within minutes without any sample preparation.

23 crowave-induced combustion was evaluated for sample preparation.

24 e entire method takes 5 h, including a 3.5-h sample preparation.

25 otocols, which do not require any additional sample preparation.

26 d, potentially as a result of differences in sample preparation.

27 f biological solid or liquid samples with no sample preparation.

28 th preliminary immunoaffinity chromatography sample preparation.

29 agents and without the digestion step in the sample preparation.

30 sign, production, encapsulation, and dTn-Seq sample preparation.

31 ble to or better than conventional stomacher sample preparation.

32 ns within the normal time scale of MALDI MSI sample preparation.

33 nts of purees can be obtained with a limited sample preparation.

34 o water background and requires little to no sample preparation.

35 ive cells is achieved in minutes without any sample preparation.

36 hemical composition were not affected by the sample preparation.

37 experimentally studied due to challenges in sample preparation.

38 of other liquid foods, without any need for sample preparation.

39 piked control to minimize mispairings due to sample preparation.

40 own of labile auxin-related compounds during sample preparation.

41 ation of analysis to less than 3 h including sample preparation.

42 experimental data in buffer solution with no sample preparation.

43 esidues using simple economic steps of field sample preparation.

44 a flowing process stream, without elaborate sample preparation.

45 down to 10 ng C) without the need of further sample preparation.

46 ons arising from batch effects and different sample preparations.

47 haracterization (variable timing), proteomic sample preparation (5-7 d), mass spectrometric data acqu

48 omplete protocol, including cell culture and sample preparation (6-7 d), SPEED imaging (4-5 h), data

49 -consuming, require bulky equipment, tedious sample preparation, a trained operator, cannot be miniat

50 ing a predefined protocol to standardize the sample preparation, acquisition, and data analysis param

51 ecovery, and artificial modifications during sample preparation also contribute to variability betwee

52 ddress this bottleneck, we combine automated sample preparation, an ultra-fast 84-second LC-MS method

53 With a simple sample preparation and a runtime as low as 11 minutes, o

54 cause this technique eliminates the need for sample preparation and allows the construction of custom

55 Recent advances in sample preparation and analysis have enabled direct prof

56 f encountered food matrices which can affect sample preparation and analysis.

57 -level Pu isotopes in water requires offsite sample preparation and analysis; therefore, new methods

58 The subsequent sample preparation and analytical processes were also fu

59 get glycan derivatization eliminates tedious sample preparation and avoids sample loss.

60 , reproducible, low-cost and high-throughput sample preparation and ChIP analysis of 96 samples (cell

61 However, even with microplate ChIP assays, sample preparation and chromatin fragmentation (which is

62 ocess, extra procedures may be needed during sample preparation and clean-up to address the issue of

63 lity, carryover, ion suppression, as well as sample preparation and consumption.

64 ative and quantitative results, standardized sample preparation and data acquisition are of highest p

65 d internal controls for the quality check of sample preparation and data acquisition, which is partic

66 Despite differences in sample preparation and data analysis, the amounts of dex

67 ructs for this purpose, honed a protocol for sample preparation and developed custom software that an

68 SD MS protein analysis involves only minimal sample preparation and does not require spectral deconvo

69 h/viability assays usually involve laborious sample preparation and expensive equipment or reagents.

70 ncreases the time and resources required for sample preparation and experimentation.

71 s chemovars were used to study the effect of sample preparation and extraction methods on terpenoid p

72 To minimize the cost of sample preparation and genotyping, most genebank genomic

73 Advancements in sample preparation and glycopeptide quantification are t

74 and an ability to handle large variations in sample preparation and image collection.

75 ce significantly static contamination due to sample preparation and improved accuracy compared to usi

76 essary for animal studies requires increased sample preparation and instrument time.

77 onance relaxometry (MRR) system with minimum sample preparation and is able to detect very low levels

78 The entire procedure including the sample preparation and LC-MS/MS takes less than 55 min,

79 ly used techniques but require sophisticated sample preparation and long incubation time.

80 thogonal as they utilized entirely different sample preparation and MS analysis workflows, targeted d

81 ased sample collection strategy with offline sample preparation and nanoLC-MS/MS to analyze proteins

82 ges of other common approaches by minimizing sample preparation and preserving endogenous modificatio

83 g an ambient ionisation process to eliminate sample preparation and provide near-instantaneous result

84 stems biology workflow employing plate-based sample preparation and rapid, single-run, data-independe

85 tomated Genetic Analyzer, performs automated sample preparation and RNA extraction, followed by ampli

86 hod employs point-of-use approaches both for sample preparation and sample measurement, demonstrating

87 athogen detection are classified in terms of sample preparation and secondary binding steps.

88 also enabling integration of SPE with other sample preparation and separation methods.

89 hy methods involve multiple dilutions during sample preparation and separation.

90 unt for measurement errors that arise during sample preparation and sequencing.

91 ten confounded with technical artifacts from sample preparation and sequencing.

92 c approaches, together with high-sensitivity sample preparation and tailored statistical data analysi

93 When sample preparation and throughput capabilities are integ

94 molecules under open-air conditions with no sample preparation and very fast sampling times.

95 een reliant upon methods utilizing extensive sample preparations and chromatographic separations and/

96 the need for Protein A purification or other sample preparations and provides unbiased information on

97 of these peptides to an efficient multistep sample preparation, and a micro-LC chromatography.

98 a lack of reference spectra, difficulties in sample preparation, and an absence of two-dimensional (2

99 SPME-GC-MS was used for sampling, sample preparation, and analyses.

100 isk of sample contamination during sampling, sample preparation, and analysis.

101 icant input proteome requirements, laborious sample preparation, and expensive equipment.

102 the analysis process, for example, sampling, sample preparation, and measurement, there is less known

103 eing up of fluorescence channels, simplified sample preparation, and the ability to re-process legacy

104 This paper reports a unified sample preparation approach for high-throughput multi-re

105 hile currently available methods for peptide sample preparation are mostly suitable for ex situ analy

106 and shape, limited training sample size, and sample preparation artifacts.

107 Here, we take a look at the advancements in sample preparation as well as in the development of tech

108 ns in whey, allowing direct analysis without sample preparation at a method runtime of 23 min.

109 cal systems; however, protein solubility and sample preparation before MS remain a bottleneck prevent

110 rs opened new perspectives for fast and easy sample preparation, but this was not fully exploited unt

111 d software on mobile devices, and circumvent sample preparation by directly targeting volatile biomar

112 r measurement of Hcrt1 in CSF with automated sample preparation by solid-phase extraction (SPE).

113 We demonstrate that this sample preparation can be parallelized for 384 samples b

114 nal standards onto the tissue section during sample preparation can be used to improve the mass accur

115 Recent studies have demonstrated that sample preparation can decrease (13) C and (15) N enrich

116 is method of detection also requires minimal sample preparation, can be done in a solution-based form

117 r research applications because of a need of sample preparation, changes of cell wall composition dur

118 r, current approaches require time-consuming sample preparation, chromatographic separations, and con

119 The sample preparation consisted of a solid-liquid extractio

120 by decreasing a major portion of the overall sample preparation cost.

121 traacetic acid (EDTA, metal chelator) during sample preparation could not only increase the extractab

122 typing studies, with detailed information on sample preparation, data acquisition and data modeling.

123 The performances of the sample preparation disc for SALDI-MS and HILIC-MS were a

124 Moreover, sample preparation does not require any digestion steps

125 LSSV does not require the extensive sample preparation (e.g., ultrafiltration or centrifugat

126 plied on our own NTA and SSA workflows where sample preparation efficiency and potential sources of e

127 Sample preparation encompassed solid-phase extraction, l

128 orm that included continuous water sampling, sample preparation (extraction), and analysis for the de

129 Harvesting period, maturity stage, sample preparation, extraction technique, and solvent ty

130 The low cost, easy sample preparation, fast response and high reproducibili

131 patient antibodies, along with the necessary sample preparation for accurate diagnoses.

132 ice (PepS) automating and accelerating blood sample preparation for bottom-up MS-based proteomics ana

133 reby providing access to highly reproducible sample preparation for common biological assays such as

134 ocal microscopy images, including optimizing sample preparation for fixed and live cells, choosing th

135 NO(2)(-) oxidation by H(2)O(2) as a part of sample preparation for HPIEC-HRMS was elaborated.

136 However, sample preparation for in situ Cryo-ET is labour-intensi

137 With improvements in the automation of sample preparation for LC-MS analysis, a challenging nex

138 of food contact materials, the importance of sample preparation for nontarget screening should be add

139 Sample preparation for our device is also straightforwar

140 s commonly used in the standard procedure of sample preparation for proteomic analysis.

141 ted out and verified with chemical analysis, sample preparation for smaller microplastic analysis is

142 te developed methodology, including a single sample preparation for the vitamins simultaneous analysi

143 al specimens is the current gold standard in sample preparation for ultrastructural analysis in X-ray

144 Established sample preparations for DNP SENS experiments on NPs requ

145 However, the sample preparations for such imaging are often onerous,

146 proposed methodology is a low cost, fast and sample preparation free methodology to highlights the EV

147 Sample preparation from human and plant specimens is a t

148 Sample preparation from minute amounts of serum was perf

149 The sample preparation generally requires only few steps; ho

150 ns provide both qualitative and quantitative sample preparation guidelines that increase the chances

151 esults show that mechanical treatment during sample preparation has a profound effect on the melting

152 Sample preparation has been the critical step that shoul

153 be developed for integrated in vivo sampling/sample preparation has been thoroughly optimized with re

154 such as time-consuming process, complicated sample preparation, high consumption of reagents and nee

155 tential to advance in situ RCA toward easier sample preparation, higher-order multiplexing, autofluor

156 ixtures in a single run without any previous sample preparation (i.e., derivatization).

157 ion, staining) that originate as a result of sample preparation; (ii) biological heterogeneity (e.g.

158 -EM single-particle analysis workflow (e.g., sample preparation, image acquisition and processing, an

159 Here, we present a complete multi-sample preparation, imaging, processing and analysis wor

160 omography phase mapping requires no specific sample preparation, in particular polishing or surface f

161 The very simple sample preparation included analytes extraction with aci

162 The sample preparation includes a washing step, allowing to

163 s are the norm but typically involve lengthy sample preparation including tissue homogenization, whic

164 untargeted metabolomics protocols, including sample preparation, instrumentation, data processing, et

165 f the limit of detection, the integration of sample preparation into the device and hence analysis di

166 The on-MALDI-target sample preparation is a single-step protocol with high d

167 When sample preparation is accounted for, cell-specific rates

168 ter mixture, from aquaculture, without prior sample preparation is demonstrated.

169 As cryo-SOFI does not require special sample preparation, it is fully compatible with conventi

170 This can make sample preparation iterative, challenging and time consu

171 The Illumina TruSeq stranded mRNA Sample Preparation kit (TruSeq) requires abundant starti

172 buffers into eluent when several commercial sample-preparation kits are used following manufacturer

173 Improper sample preparation leads to detrimental cascades, result

174 This two-step sample preparation led to excellent extraction efficienc

175 r ultrastructure but requires time-consuming sample preparation, limiting throughput.

176 s on instrumentation that requires extensive sample preparation, long run times, and is destructive t

177 of the analysis and minimal requirement for sample preparation make PSI-MS a promising avenue for fu

178 only applied in microplastic research during sample preparation, may also be mistaken for PE.

179 We report a miniaturized filter aided sample preparation method (micro-FASP) for low-loss prep

180 timescales, through an optimal design of the sample preparation method and AFM parameters for faster

181 Here we report a time-resolved sample preparation method for cryo-electron microscopy (

182 hilized, isothermal assays with a simplified sample preparation method independent of nucleic acid ex

183 A new sample preparation method is proposed for the extraction

184 However, this lipid-based approach used a sample preparation method that required more than a work

185 lved terahertz spectroscopy along with a new sample preparation method to determine the photoconducti

186 s spectrometry (MS/HRMS) assay with a simple sample preparation method was developed.

187 A low-cost, reflective sample preparation method with stable particle mounting

188 This paper describes a simple, fast sample preparation method without the need for sample fr

189 A sample preparation method, QuEChERS extraction combined

190 For this purpose, a bulk sample-preparation method was developed, allowing a high

191 ication of membrane components, and then the sample preparation methodologies and data analysis strat

192 ses of pesticide in fruits using QuEChERS as sample preparation methodology.

193 imized by testing different types of fibers, sample preparation methods and amounts, extraction tempe

194 because of inherent limitations of existing sample preparation methods and instrumentation.

195 Our results demonstrate that sample preparation methods may significantly impact the

196 For example, we do not know the optimal sample preparation methods or imaging conditions to coun

197 complex instrumentation involving extensive sample preparation methods, especially when sensing is p

198 Sample preparation methodsforcereal digestion were evalu

199 igh sensitivity, fast response time, minimal sample preparation, miniaturization and ability for real

200 When fully integrated with a sample preparation module, this diagnostic system will e

201 ochemical analysis are its low cost, minimal sample preparation, non-destructive nature and substanti

202 estigations because of its low cost, minimal sample preparation, non-destructive nature and substanti

203 synthetic urine in less than 25 min without sample preparation nor concentration.

204 n overview of NA-POCT platforms in regard to sample preparation of NA, NA amplification, NA detection

205 ing optimized proteoliposome isolation, cryo-sample preparation on graphene grids, and an efficient p

206 t manner without the need for time-consuming sample preparation or derivatization.

207 No sample preparation or internal standards were needed for

208 detection is often a necessary and critical sample preparation or purification step in many lab-on-a

209 o a person's metabolism without the need for sample preparation or sample collection.

210 Combined with the simple sample preparation, our method represents a valuable too

211 a is polluted with off-sample ions caused by sample preparation, particularly by the MALDI (matrix-as

212 pectrometry (MS)-based proteomics, including sample preparation, peptide separation, and data analysi

213 cibility with regards to both instrument and sample preparation performance.

214 However, nucleic acid (NA) sample preparation preceding dNAAT is generally laboriou

215 affinity capture was employed as a universal sample preparation procedure applicable to both full-len

216 A fast sample preparation procedure based on use of infrared (I

217 odologies, a consistent instrument model and sample preparation procedure is no longer a requirement.

218 m cell suspension without needing additional sample preparation procedures (e.g., molecular tagging)

219 t matrix) often utilized for optimization of sample preparation procedures and also instrumental cond

220 response testing methods by both simplifying sample preparation procedures and making a benchtop read

221 ct for the difference between instruments or sample preparation procedures.

222 The sample preparation process takes 2-3 d, whereas the time

223 The whole sample preparation process was carried out at less than

224 A systematic sample preparation process was optimized, which comprise

225 Following nanoPOTS sample preparation, protein digests from single cells we

226 th a prototype effective and straightforward sample preparation protocol and delivers reliable peptid

227 as all of the advantages of the filter aided sample preparation protocol are maintained.

228 We optimized the sample preparation protocol by using a set of 6 mobile c

229 The sample preparation protocol included a simple and rapid

230 In the present work, sample preparation protocol is reported for the simultan

231 analytical modalities through this universal sample preparation protocol offers the ability to study

232 An efficient sample preparation protocol with 80% acetonitrile as the

233 ches by speeding up the classic filter aided sample preparation protocol, FASP, from overnight to 2.5

234 The two labs used the same silylation sample preparation protocols but different instrumentati

235 and technique-demanding than other advanced sample preparation protocols in the field.

236 owever, requires narrowly-defined and strict sample preparation protocols.

237 onventional separation columns or additional sample preparation protocols.

238 Sample preparation, quantitative LC-tandem MS (LC-MS/MS)

239 Sample preparation relies on immunoextraction in 96-well

240 ing, need relatively large equipment, demand sample preparation, require a skilled operator, and lack

241 uent (demonstrating the reduction of tedious sample preparation requirements for biological samples p

242 hes will be useful to diversify the range of sample preparation schemes and analytical methods enable

243 According to the type of sample preparation, sensitivity of the experiment, labor

244 f food and their interactivity, an effective sample preparation should be employed to extract these c

245 igher sensitivity and repeatability, minimum sample preparation, simplicity, and portability.

246 The aim of our study was to improve sample preparation so that MSI could provide comprehensi

247 The sample preparation step involved membrane bag liquid-pha

248 The method required a simple sample preparation step that consisted of dissolution or

249 ith pattern recognition methods, without any sample preparation step.

250 However, such approach requires several sample preparation steps and a dedicated laboratory envi

251 The assay does not require off-chip sample preparation steps and minimizes human involvement

252 scheme involves the use of minimum reagents/sample preparation steps, has appreciable response in pr

253 t force nonoptimal modifications to upstream sample preparation steps, limit the throughput of high-v

254 med without the use of an organic solvent or sample preparation steps, with only the sample dilution

255 ls be sacrificed and tend to involve tedious sample preparation steps.

256 evant and does not require any enrichment or sample preparation steps.

257 s time-consuming and labor-intensive offline sample preparation steps.

258 ose directly from human whole bloodthout any sample preparation steps.

259 ersal" indicator, although considerations in sample preparation, storage, and applicability are discu

260 rfering compounds found in urine necessitate sample preparation strategies that are currently not sui

261 Here, we present two sample preparation strategies to approach the wide dynam

262 However, many of these devices require sample preparation such as plasma separation to remove c

263 m (FIB) has a broad scope of applications in sample preparation such as protective layer deposition,

264 own platform by incorporating a microfluidic sample preparation system, termed nanoPOTS (nanodroplet

265 The sample preparation takes various time frames, ranging fr

266 anually, thus creating the need for a simple sample preparation technique and a facile coupling strat

267 digestion procedure is chosen as an optimal sample preparation technique for the TXRF analysis of te

268 Here, we explore sample preparation techniques relevant to a range of cli

269 Different sample preparation techniques, e.g. suspension, open ves

270 nalyses of interacting microbes among tested sample preparation techniques.

271 PE) is a general preconcentration method for sample preparation that can be performed on a variety of

272 hy (nanoLC) and, for histone proteins, a 2-d sample preparation that includes histone purification, d

273 Because of the simplicity of the sample preparation, the small quantity of material neede

274 ogy is simple and does not require extensive sample preparation, the throughput of such an approach i

275 ally available generic reagents and requires sample preparation time of less than 7 h.

276 nchmarking studies, OTTO showed accuracy and sample preparation times comparable to manual qPCR.

277 ng because of the difficulties in optimizing sample preparation to acquire critical MS data and detec

278 ry, fluorescent cell barcoding and automated sample preparation to characterize ex vivo signaling net

279 py, this protocol typically takes 2-3 d from sample preparation to data acquisition, with an addition

280 automated workflow for CIU experiments, from sample preparation to data interpretation using online s

281 terogeneous biological mixtures, methods for sample preparation to detect (1)H-, (13)C-, (15)N-, and

282 This study also examined the effect of the sample preparation to determine destructive factors infl

283 PCR solution, enabling direct transfer from sample preparation to dNAAT.

284 ctrometry (1D LC-MS/MS) workflow (i.e., from sample preparation to HCP identification) to be complete

285 detection as well as demonstrates SPME as a sample preparation tool for nucleic acid analysis in pla

286 ny target panel of genes without specialized sample preparation using any computer and a suitable GPU

287 rce of gelatin with 100% success without any sample preparation using FTIR-ATR technique.

288 f thiol-containing polymer chain-ends during sample preparation using THF as solvent.

289 greatly reduced instrument-to-instrument and sample preparation variabilities.

290 Sample preparation was carried out in a 10 000 class cle

291 le laboratory gloves or reagents used during sample preparation was investigated.

292 tracts from reference bacterial strains, and sample preparation was optimized using mouse brain tissu

293 The crucial step in the sample preparation was the total dissolution/reprecipita

294 To monitor detector performance and sample preparation, we use egg white as an external cont

295 nucleic acid extraction is the first step of sample preparation, which remains one of the main challe

296 or less may be achieved by combining careful sample preparation with improved instrumentation.

297 Here, we combine MIL-based sample preparation with isothermal amplification and det

298 This study demonstrated that the new sample preparation with magnetic nanoparticles could pot

299 By combining optimized sample preparation with subatmospheric pressure MALDI, w

300 ring each step of a conventional in-solution sample preparation workflow using bicinchoninic acid (BC