ライフサイエンスコーパス: saporin

1 ain cholinergic deficits (induced by 192 IgG-saporin).

2 VDB) cholinergic neurons produced by 192 IgG-saporin.

3 droxylase antibodies conjugated to the toxin saporin.

4 y lesioned with an immunotoxin (IT), 192 IgG-saporin.

5 cortical infusion of the immunotoxin 192 IgG-saporin.

6 ted with the selective cytotoxin substance P-saporin.

7 n composed of basic fibroblast growth factor-saporin.

8 gic neurons in NDB unilaterally with 192-IgG-saporin.

9 oned with the cholinergic immunotoxin 192IgG-saporin.

10 cortical infusion of the immunotoxin 192 IgG-saporin.

11 rebroventricularly with 4 micrograms 192 IgG-saporin.

12 was found in aged rats treated with 192-IgG-saporin.

13 brain with the ribosome-inactivating protein saporin.

14 scular injection of cholera toxin-conjugated saporin.

15 ansported catecholamine immunotoxin, antiDBH-saporin.

16 ced after a ventricular injection of 192 IgG-saporin.

17 used with the selective cholinotoxin 192 IgG-saporin (0.005 microgram/0.5 microliter/site) into the f

18 re induced in rats with infusions of 192 IgG-saporin (0.1 microg/0.5 microl per side).

19 y nerve growth factor receptor conjugated to saporin (192 IgG-saporin), lesioned rats were processed

20 of the highly selective immunotoxin 192 IgG-saporin (192-sap) into the ventricular system.

21 ulness were not different between hypocretin-saporin, 192 IgG-saporin, or saline-treated rats.

22 Rats received PBS or the immunotoxin 192IgG-saporin (192Sap) intracerebroventricularly at two doses

23 linked to the ribosome-inactivating protein saporin-6 (rFGF2-SAP) on vascular SMC cytotoxicity and n

24 rious concentrations of rFGF2-SAP, FGF2, and saporin-6 (SAP).

25 tibody to DBH coupled by a disulfide bond to saporin (a ribosome inactivating protein), has been show

26 The authors used 192 IgG-saporin, a lesioning agent selective for basal forebrain

27 tion of guanidinoneomycin to the plant toxin saporin, a ribosome-inactivating agent, results in prote

28 ng the intraseptal administration of 192-IgG-Saporin, a specific cholinergic neurotoxin, we have foun

29 We studied the effects of 192 IgG-saporin, a specific immunotoxin for the NGF receptor-pos

30 ith saline or anti-dopamine-beta-hydroxylase-saporin, a toxin that destroys noradrenergic neurons of

31 linked antibodies conjugated to biotinylated saporin, a toxin unable to cross cell membranes.

32 roventricular anti-dopamine beta-hydroxylase/saporin, a treatment that destroys a majority of noradre

33 This protective effect of dermorphin-saporin against SNL-induced pain was blocked by beta-fun

34 made after intrathecal infusion of vehicle, saporin alone, or SP-SAP.

35 tion of 5, 10, and 20 micrograms of anti-DBH-saporin (alpha-DBH-sap) into rats.

36 -hydroxydopamine (6-OHDA), a toxin devoid of saporin, also damaged NTS catecholamine neurons but did

37 eeks after intraventricular injection of 192-saporin, an immunotoxin directed at the low affinity neu

38 a partial immunolesion to CBFNs with 192 IgG-saporin, an immunotoxin selectively taken up by p75NTR-b

39 Data indicated that saporin and abrin II shared one pattern, while ricin and

40 e mu-opioid agonist dermorphin; unconjugated saporin and dermorphin were used as controls.

41 e loss of SPR-positive cells, a conjugate of saporin and the more potent and peptidase-resistant SP a

42 tested in combination with a protein toxin, saporin, and a significant reduction in cell viability w

43 including Ricinus communis agglutinin (RCA), saporin, and abrin II.

44 electively killed by i.c.v. injection of 192-saporin, and cerebellar Purkinje cells which are killed

45 ection of a retrogradely transported form of saporin, and examined the morphology of contralateral SN

46 rats by bilateral local injection of orexin-saporin, and polysomnography was performed to measure ba

47 to dopamine-beta-hydroxylase conjugated with saporin (anti-DBH-SAP) damages catecholamine neurons in

48 NTS DBH neurons with anti-DBH conjugated to saporin (anti-DBH-SAP).

49 e antibody conjugated to the ribosomal toxin saporin (anti-DH-SAP).

50 body to the serotonin transporter (SERT) and saporin (anti-SERT-SAP; 1 microm) to kill serotonergic n

51 Using ribonuclease A (RNase A) and saporin as two representative cytotoxic proteins, the co

52 rtical infusions of the cholinotoxin 192 IgG-saporin, attenuated the beneficial effects of NMDA on dS

53 Using a selective saporin-based lesion, we demonstrate that the loss of gu

54 Using saporin-based lesions and transcriptomics, we investigat

55 pressing mu-opioid receptors with dermorphin-saporin, blocked tactile and thermal hypersensitivity, a

56 RVM dermorphin-saporin, but not dermorphin or saporin, significantly de

57 RVM microinjection of dermorphin-saporin, but not of dermorphin or saporin, in animals pr

58 log [Sar(9), Met(O(2))(11)] Substance P (SSP-saporin) caused negligible nonspecific damage at the inj

59 itioning with anti-CD45 mAbs conjugated with saporin CD45 (CD45-SAP).

60 However, hypocretin-saporin completely eliminated hippocampal theta activity

61 Using an IB(4)-saporin conjugate (IB(4)-SAP), we examined the contribut

62 he neurotoxin saporin (SAP), or the orexin-2-saporin conjugate (OXSAP) in the lateral hypothalamus.

63 region (RTN/Ppy), we injected a substance P-saporin conjugate (SP-SAP; 0.1 pmol in 100 nl) to kill N

64 This ipRGC immunotoxin, consisting of saporin conjugated to a melanopsin polyclonal antibody,

65 ere targeted with a single microinjection of saporin conjugated to the mu-opioid agonist dermorphin;

66 fects of the hypocretin-saporin with another saporin conjugated toxin, 192 IgG-saporin, that lesions

67 (NK1R)-ir and were selectively destroyed by saporin conjugated with an NK1R agonist (SSP-SAP).

68 me inactivation approach through delivery of saporin-conjugated anti-vesicular GABA transporter antib

69 We used a saporin-conjugated antibody against CD11b to reduce the

70 blating microglia in the spinal cord using a saporin-conjugated antibody to Mac1, we demonstrate a ca

71 nally transported catecholamine immunotoxin, saporin-conjugated antiserum to dopamine-beta-hydroxylas

72 s triple lesion of these neurons using three saporin-conjugated neurotoxins.

73 Using HLA-B*14/peptide-saporin-conjugated tetramers, we show that HLA-B*14-EL9

74 th DS (1.0 or 2.0 pmol/200 nl/side) or blank-saporin control (BS, 200 nl/side) into the VTA.

75 cular application of the mitochondrial toxin saporin, coupled to the antibody directed against dopami

76 We show that saporin-coupled tetramers can delete islet-specific gluc

77 ning them with antidopamine beta-hydroxylase-saporin (DBH-SAP) injected via the 4th ventricle.

78 mine beta-hydroxylase antibody conjugated to saporin, DBH-Sap), and measure resulting electrophysiolo

79 injury (SNI) model of neuropathic pain, NPY-saporin decreased mechanical and cold hypersensitivity,

80 NPY-saporin decreased spinal Y1R immunoreactivity but did no

81 192-saporin decreased the number of correct choices and incr

82 Using intrathecal dermorphin-saporin (Derm-sap) to selectively destroy MOR-expressing

83 192 IgG-saporin did not affect the total daily amounts but alter

84 RVM dermorphin or saporin did not alter SNL-induced experimental pain, and

85 RVM dermorphin, saporin, or dermorphin-saporin did not change baseline hindpaw sensitivity to n

86 pressing mu-opioid receptors with dermorphin-saporin did not prevent the onset of SNL-induced tactile

87 ction, we employed the neurotoxin dermorphin-saporin (DS) to selectively lesion VTA GABA neurons prio

88 xylase antibody conjugated to the neurotoxin saporin (DSAP) or saline vehicle was microinjected into

89 The toxin-antibody complex anti-d(beta)h-saporin (DSAP) selectively destroys d(beta)h-containing

90 When 25A11 was coupled to the cytotoxin saporin either directly or via a secondary antibody, bot

91 cells in vitro when coupled to the cytotoxin saporin either directly or via a secondary antibody.

92 By 10 d, saporin eliminated staining for choline acetyltransferas

93 One to 12 weeks after injection of SSP-saporin, extracellular electrophysiological field respon

94 njury in rats pretreated with RVM dermorphin-saporin failed to elicit the expected increase in sensit

95 icacy of [Sar(9), Met(O(2))(11)] Substance P-saporin for producing a selective and spatially extensiv

96 ven GABAergic lesions of the MSDB using GAT1-saporin (GAT1-SAP) and examined on spontaneous explorati

97 he selective cholinergic immunotoxin 192 IgG-saporin have demonstrated that lesions of the cholinergi

98 e medial septum had been destroyed by mu P75-saporin, human MGE-like progenitors, but not ventral spi

99 We used the murine-p75-saporin immunotoxin (mu-p75-sap) to induce selective les

100 R agonist dermorphin conjugated to the toxin saporin in the vicinity of ITC neurons caused a 34% redu

101 dermorphin-saporin, but not of dermorphin or saporin, in animals previously undergoing SNL showed a t

102 192 IgG-saporin-induced lesions of basal forebrain cholinergic n

103 Saporin-induced septal lesions produced a significant re

104 Simultaneously, some saporin-injected rats were given implants containing tes

105 RVM dermorphin-saporin injection prevented the maintenance, but not the

106 atment, SNB motoneurons contralateral to the saporin injection were retrogradely labeled with horsera

107 cortical regions was seen 12 weeks after 192-saporin injection with no further change up to 100-week

108 sterone replacement beginning at the time of saporin injection.

109 rvated hippocampus at 4, 8 and 12 weeks post Saporin injection.

110 ons of the anti-neuronal immunotoxin 192-IgG saporin into either the hippocampus or the cingulate cor

111 ts of intraparenchymal injections of 192 IgG-saporin into either the MS or NB on the organization of

112 fferents as a result of infusions of 192 IgG-saporin into the basal forebrain show persistent impairm

113 Local injections of the neurotoxin SP-saporin into the basolateral amygdala (BLA) are reported

114 Injection of 100, 237.5 or 375 ng of 192-saporin into the medial septum produced dose-related def

115 ections of the selective immunotoxin 192 IgG-saporin into the medial septum/vertical limb of the diag

116 infusing the cholinergic immunotoxin 192 IgG-saporin into the NBM.

117 ion of the specific immunotoxin, anti-p75NTR-saporin into the nucleus basalis.

118 Site-specific injection of IgG 192-saporin is a useful approach to explore the functions of

119 192-IgG saporin is an anti-neuronal immunotoxin that combines th

120 Ricin A-chain (RTA) and saporin-L1 (SAP) catalyze adenosine depurination of 28S

121 imics of small oligonucleotide substrates of saporin-L1 are powerful, slow-onset inhibitors when aden

122 We characterized the catalytic properties of saporin-L1 from Saponaria officinalis (soapwort) leaves,

123 Transition state analogues of saporin-L1 have potential in cancer therapy that employs

124 Saporin-L1 inhibition of rabbit reticulocyte translation

125 ave potential in cancer therapy that employs saporin-L1-linked immunotoxins.

126 CD117-antibody-drug-conjugate (CD117-ADC) to saporin leads to > 99% depletion of host HSCs, enabling

127 The present experiments used the 192IgG-saporin lesion model of AD to evaluate the actions of ga

128 misphere and a 192 immunoglobulin G (192IgG)-saporin lesion of cholinergic neurons in the contralater

129 creased rapid-eye-movement sleep in orexin-B saporin lesioned rats supporting its potential therapeut

130 efulness was also not affected in hypocretin-saporin lesioned rats.

131 ceived intracerebroventricular injections of saporin (lesioned) or saline (controls) and were tested

132 ctor receptor conjugated to saporin (192 IgG-saporin), lesioned rats were processed simultaneously wi

133 quently, these results support the use of SP-saporin lesions as a useful technique to study the role

134 subgroups in the BLA might be targeted by SP-saporin lesions has not been established.

135 f the nBM cholinergic system through 192 IgG-saporin lesions impairs early acquisition of learning se

136 iments, the performance of rats with 192 IgG-saporin lesions of both hippocampal and neocortical chol

137 Prior studies showed that 192 IgG-saporin lesions of cholinergic input to the hippocampus

138 Rats with 192 IgG-saporin lesions of the NBM were assessed for perseverati

139 Rats received unilateral sham or 192 IgG-saporin lesions of the NBM.

140 Rats with 192 IgG-saporin lesions of the nucleus basalis magnocellularis (

141 Rats with bilateral 192 IgG-saporin lesions to all basal forebrain cholinergic nucle

142 Rats with bilateral 192 IgG-saporin lesions to the nucleus basalis magnocellularis (

143 Hence, SP-saporin lesions would ablate all interneurons in the BLA

144 al nerve ligation, and this was abolished by saporin linked anti-p75(NTR) treatment.

145 Others received intrathecal injection of saporin linked to an antibody to the neurotrophin recept

146 the mitotoxin basic fibroblast growth factor-saporin more than 10-fold, thus allowing tumor cell kill

147 d either by microinjecting NPY conjugated to saporin (NPY-SAP) bilaterally into the Arc to kill NPY r

148 ntracerebroventricular injections of 192 IgG-saporin, NPY-immunolabeled neurons in the hilus of the d

149 SI cholinergic lesions, induced with 192 IgG saporin, on behavioral measures of aversive states in ra

150 B(4)-SAP-treated, but not control (saline or saporin only), rats.

151 other protein synthesis inhibitors, such as saporin or cycloheximide.

152 rmance in this task, the immunotoxin 192 IgG-saporin or its vehicle was infused into the area of the

153 uction in cell viability was measured versus saporin or photosensitiser treatment alone.

154 reatment of A549 cells or control cells with saporin or Pseudomonas exotoxin A whose intracellular me

155 wing intraseptal injection of either 192-IgG-saporin or saline, testing began in a battery of behavio

156 Treatment with isolectin B4 (IB4)-saporin or SSP-saporin (which deplete IB4(+) and peptide

157 activation is not dependent upon reduction (saporin) or only partially dependent upon it (Pseudomona

158 RVM dermorphin, saporin, or dermorphin-saporin did not change baseline h

159 ifferent between hypocretin-saporin, 192 IgG-saporin, or saline-treated rats.

160 ucleus region was destroyed with substance P-saporin prior to lentivirus injection into the rostral v

161 croinjection of a conjugate of native SP and saporin produced significant nonspecific damage at conce

162 s, although intraseptal injection of 192-IgG-saporin produced similar reductions of ChAT activity, pe

163 However, the BF-saporin rats were hypersensitive to oxotremorine-induced

164 Intracortical infusions of 192 IgG-saporin reduced basal cortical ACh efflux by 47% of sham

165 192 IgG-saporin reduced theta activity, a finding consistent wit

166 septal inputs using the immunotoxin 192 IgG-saporin reduces the number of interneurons containing ne

167 The degree of lesion produced by 192 IgG-saporin relative to controls was compared using three in

168 he selective cholinergic immunotoxin 192 IgG-saporin resulted in a >80% decrease in the number of lar

169 of these neurons by the selective toxin SSP-saporin resulted in a complete disruption of ejaculatory

170 ugation of the sialoside probes to the toxin saporin resulted in toxin uptake and toxin-mediated kill

171 a relatively small concentration of 192 IgG-saporin, resulted in a significant impairment in sustain

172 CD11b and the ribosome-inactivating protein saporin, resulted in reduced microglia staining, reducti

173 rathecal administration of an NPY-conjugated saporin ribosomal neurotoxin that spares the central ter

174 l injection of specific cholinotoxin 192-IgG saporin (SAP) +/- intraperitoneal injection of N-[chloro

175 cholaminergic neurons) was done by injecting saporin (SAP) conjugated with a selective NK1R agonist [

176 e produced by bilateral infusions of 192 IgG-saporin (SAP) into the basal forebrain and/or 6-hydroxyd

177 The plant cytotoxin saporin (SAP) is a potent ribosome-inactivating protein.

178 received injections of 100 or 375 ng 192-IgG saporin (SAP) or artificial CSF (C) into the medial sept

179 Rats were administered 192-IgG saporin (SAP) or vehicle into the medial septum-vertical

180 e, zebrafish Kiss1 peptide was conjugated to saporin (SAP) to selectively inactivate Kiss-R1-expressi

181 forebrain cholinergic neurons by conjugating saporin (SAP), a ribosome-inactivating protein, to a rat

182 A single dose of the immunotoxin, CD45-saporin (SAP), enabled efficient (>90%) engraftment of d

183 Rats were administered saline, 192-IgG saporin (SAP), or kainic acid (KA) into the MSDB and the

184 ry secretion, we injected DSAP, unconjugated saporin (SAP), or saline bilaterally into the paraventri

185 er phosphate-buffered saline, the neurotoxin saporin (SAP), or the orexin-2-saporin conjugate (OXSAP)

186 jugated to the ribosome-inactivating protein saporin (SAP).

187 ing either quisqualic acid (QUIS) or 192 IgG-saporin (SAP).

188 d to the ribosome-inactivating protein (RIP) saporin (SAP).

189 n for neurokinin-3 expressing neurons [NK(3)-saporin (SAP)] into the rat arcuate nucleus.

190 iving intrahippocampal injections of 192 IgG-saporin (SAP-HPC), fimbria-fornix lesions (FF), or sham

191 gated soluble DC-HIL receptor with the toxin saporin (SAP; DC-HIL-SAP) and showed it to bind activate

192 192-IgG-saporin selectively destroys cholinergic neurons and ter

193 aged rats, intraseptal injection of 192-IgG-saporin selectively reduced ChAT activity in the hippoca

194 Rats treated with OX7-saporin showed impaired reacquisition of excitatory cond

195 VM dermorphin-saporin, but not dermorphin or saporin, significantly decreased cells expressing mu-opi

196 he selective cytotoxin conjugate substance P-saporin (SP-SAP).

197 We injected (2 x 100 nl) (a) substance P-saporin (SP-SAP; 1 microm) to kill NK1R-expressing neuro

198 arrier impermeable toxin, stable substance P-saporin (SSP-SAP), which ablates cells expressing NK rec

199 Injection of the neurotoxin saporin-substance P (SSP-SAP) into the retrotrapezoid nu

200 igands and the ribosome inactivating protein saporin that specifically target Substance P receptor-ex

201 th another saporin conjugated toxin, 192 IgG-saporin, that lesions only the cholinergic neurons in th

202 ed against dopamine beta hydroxylase (DbetaH-saporin), the analgesic and sedative actions of N(2)O we

203 l immunotoxin was constructed by conjugating saporin, the ribosome-inactivating protein toxin, to an

204 When conjugated to the model toxin saporin, three of our nanobodies caused growth inhibitio

205 e used cholera toxin B-subunit conjugated to saporin to demyelinate the rat lumbar spinal cord, remov

206 th a single RVM microinjection of dermorphin-saporin to eliminate cells that drive descending facilit

207 anti-dopamine-beta-hydroxylase conjugated to saporin to lesion hindbrain catecholamine neurons.

208 tion of sleep drive by administering 192-IgG-saporin to lesion the BF cholinergic neurons and then me

209 ventricular infusions of the immunotoxin OX7-saporin to selectively destroy Purkinje cells throughout

210 Targeting saporin to Siglec-8 consistently caused extensive cell d

211 We used NMB-saporin to specifically eliminate NMBR-expressing neuron

212 ith the cytotoxin, saporin, using either CCK-saporin to target CCK receptor expressing cells, or derm

213 CCK receptor expressing cells, or dermorphin-saporin to target MOR expressing cells, resulted in conc

214 Anti-dopamine beta-hydroxylase-saporin toxin (DbetaH-SAP) was used to selectively lesio

215 For this purpose, saporin toxin conjugated to an antibody against DbH was

216 s of the stria terminalis (BNST), vehicle or saporin toxin conjugated to an antibody against dopamine

217 We utilize a saporin toxin conjugated to the hypocretin receptor bind

218 re eliminated by previous infusion of 192IgG-saporin toxin into the medial septum.

219 of either phosphate buffer (PBS) or 192 IgG-saporin (Toxin), 3.6 micrograms rat-1, a cholinergic imm

220 hese multivalent ligands with auristatin and saporin toxins are efficiently internalized via hCD22 re

221 t analgesia, was markedly attenuated in both saporin-treated groups.

222 gonadally intact saporin-treated males, and saporin-treated males who had been castrated 6 weeks pre

223 ed in normal control males, gonadally intact saporin-treated males, and saporin-treated males who had

224 SSP-saporin-treated rats exhibited relatively normal respons

225 issues from these DLF-lesioned or dermorphin-saporin-treated SNL rats did not exhibit enhanced capsai

226 DbetaH-saporin treatment eliminated nearly all of the catechola

227 havioral tests analyzed, intraseptal 192-IgG-saporin treatment had no effect in mature animals.

228 Intrathecal bombesin-saporin treatment reduced the number of GRPR+ neurons by

229 Ten days after 192 IgG-saporin treatment, ChAT activity decreased to 35-50% of

230 Four weeks after saporin treatment, SNB motoneurons contralateral to the

231 d cell lesion in the RVM with the cytotoxin, saporin, using either CCK-saporin to target CCK receptor

232 The ribosome-inhibiting protein saporin was conjugated to a Siglec-8-specific antibody t

233 ance P and the ribosome-inactivating protein saporin was infused into the spinal cord, it was interna

234 n selective for cholinergic neurons, 192 IgG-saporin, was examined in rats trained to perform 2 versi

235 ntracerebroventricular injections of 192 IgG-saporin were trained on object discrimination problems a

236 e selective cholinergic immunotoxin, 192-IgG-saporin, were investigated in mature (6-month-old) and a

237 he diagonal band of Broca, made with 192 IgG-saporin, were not impaired in acquiring the same olfacto

238 ellar Purkinje cells which are killed by OX7-saporin, were not killed by alpha-DBH-sap.

239 urons with intrathecal injection of bombesin-saporin, whereas intrathecal GRP-induced itch response r

240 tment with isolectin B4 (IB4)-saporin or SSP-saporin (which deplete IB4(+) and peptidergic nociceptor

241 anding specificity against another biotoxin, saporin, which has mechanism of action similar to ricin.

242 -saporin, which is targeted at Thy1, and 192-saporin, which is targeted at the low affinity neurotrop

243 They are OX7-saporin, which is targeted at Thy1, and 192-saporin, whi

244 We contrast the effects of the hypocretin-saporin with another saporin conjugated toxin, 192 IgG-s

245 Conjugation of the plant toxin saporin with basic fibroblast growth factor has increase

246 ndicating specific interaction of dermorphin-saporin with the mu-opioid receptor.