ライフサイエンスコーパス: scanning mutagenesis

1 one at a time, with Cys or Ala (Cys- and Ala-scanning mutagenesis).

2 ed by cross-competition analysis and alanine-scanning mutagenesis.

3 3 were an engineered salt bridge and leucine scanning mutagenesis.

4 e epitopes was accomplished by using alanine scanning mutagenesis.

5 sidues 195 to 244) was evaluated by cysteine scanning mutagenesis.

6 G2, we performed point mutations and alanine-scanning mutagenesis.

7 f the arm to folding was obtained by alanine scanning mutagenesis.

8 s in mammalian cell membranes using cysteine scanning mutagenesis.

9 asmic tail were identified by single-alanine scanning mutagenesis.

10 d ESCRT pathways, which we tested by alanine-scanning mutagenesis.

11 idues within this loop, we performed alanine-scanning mutagenesis.

12 genesis, carbohydrate shielding, and alanine scanning mutagenesis.

13 , Thr978, as an essential residue by alanine scanning mutagenesis.

14 a interface residues were chosen for alanine scanning mutagenesis.

15 ues, was generated to permit future cysteine-scanning mutagenesis.

16 peptide agonists were studied using alanine-scanning mutagenesis.

17 to GrpE-DnaK binding were probed by alanine-scanning mutagenesis.

18 rol of specific traits, we performed alanine-scanning mutagenesis.

19 tracellular region of DDR1 by using cysteine-scanning mutagenesis.

20 ved switch II residues by performing alanine-scanning mutagenesis.

21 the entire A2 surface when mapped by homolog-scanning mutagenesis.

22 We subjected HD5 to comprehensive alanine scanning mutagenesis.

23 For this study, by employing alanine-scanning mutagenesis, (125)I-SDF-1alpha competition bind

24 ing article, we demonstrate, through alanine-scanning mutagenesis, a key role for extracellular loop

25 this molecular environment, we used alanine-scanning mutagenesis across residues 660 to 680 in the M

26 Alanine-scanning mutagenesis along the entire length of Aichi vi

27 we performed extensive substituted cysteine-scanning mutagenesis analysis of the C-terminal region o

28 Alanine scanning mutagenesis analysis shows that four conserved

29 In combination with alanine scanning mutagenesis and activity measurements we unveil

30 athway signaling, using experimental alanine scanning mutagenesis and also the FTMap method for compu

31 TM3) in NKCC1 using cysteine- and tryptophan-scanning mutagenesis and analyzed our results in the con

32 ab5-p110beta interface, we performed alanine-scanning mutagenesis and analyzed Rab5 binding with an i

33 70 through Ser-293, was analyzed by cysteine-scanning mutagenesis and chemical modification.

34 Using alanine scanning mutagenesis and crystallographic approaches, we

35 dues of a prototypic PPPSP motif via alanine scanning mutagenesis and demonstrate that each of the fi

36 Cysteine scanning mutagenesis and EPR spectroscopy identified Cys

37 TDPVLSALI(152)) in activity by using alanine scanning mutagenesis and examining salt tolerance in sod

38 sing a combination of site-directed cysteine-scanning mutagenesis and fluorescein-5-maleimide labelin

39 g site of AFABP/aP2 a combination of alanine-scanning mutagenesis and fluorescence resonance energy t

40 ARS309, ARS319, ARS606 and ARS607) by linker scanning mutagenesis and found that sequences adjacent t

41 Scanning mutagenesis and functional characterization of

42 ing at different DNA sequences using alanine-scanning mutagenesis and identified several key residues

43 Alanine-scanning mutagenesis and kinetic analysis identified thr

44 Alanine scanning mutagenesis and kinetic analysis revealed that

45 tructure and function, we performed cysteine-scanning mutagenesis and methanethiosulfonate (MTS) acce

46 Peptide alanine-scanning mutagenesis and modeling of receptor-bound sCT

47 Furthermore, scanning mutagenesis and molecular modeling studies of t

48 Alanine-scanning mutagenesis and neutralization escape mapping a

49 esults are consistent with previous cysteine-scanning mutagenesis and NMR analyses of PS1 and provide

50 Consistent with this idea, cysteine-scanning mutagenesis and NMR studies revealed a number o

51 We performed saturated alanine scanning mutagenesis and other amino acid substitutions

52 We used alanine-scanning mutagenesis and patch clamp photometry to study

53 lypeptide sequence have now been analyzed by scanning mutagenesis and SCAM.

54 s of the D-loop (in solution) using cysteine scanning mutagenesis and site-directed labeling.

55 EGF for these three antibodies using alanine-scanning mutagenesis and structural analyses.

56 velope function has been examined by alanine scanning mutagenesis and subsequent characterization of

57 velope function has been examined by alanine-scanning mutagenesis and subsequent characterization of

58 enous Cys residues were subjected to Ala/Thr scanning mutagenesis and subsequent exposure to affinity

59 ed this region to both alanine- and arginine-scanning mutagenesis and tested mutants for DNA binding

60 glucose transporter was analyzed by cysteine-scanning mutagenesis and the substituted cysteine access

61 elix in this model, was examined by cysteine-scanning mutagenesis and the substituted cysteine access

62 hypothetical model, was examined by cysteine-scanning mutagenesis and the substituted cysteine access

63 n 5-HT3A subunit using alanine- and arginine-scanning mutagenesis and the substituted cysteine access

64 Using scanning mutagenesis and total internal reflection fluor

65 Using in vitro assembly, alanine scanning mutagenesis, and biophysical analyses, we have

66 Using an in vitro assembly assay, alanine scanning mutagenesis, and biophysical techniques (circul

67 ystallography, shotgun reversion/replacement scanning mutagenesis, and computational analysis, we des

68 mbination of cysteine cross-linking, alanine-scanning mutagenesis, and computational simulations, tha

69 mbrane binding properties, performed alanine-scanning mutagenesis, and identified residues important

70 l analyses, peptide binding analysis, linker-scanning mutagenesis, and nuclear magnetic resonance (NM

71 ng computational docking algorithms, alanine scanning mutagenesis, and surface plasmon resonance, we

72 guide, we undertook a comprehensive alanine-scanning mutagenesis approach at the TCR-pMHC-I interfac

73 A glutamine scanning mutagenesis approach reveals that Asp91, His93,

74 Here we used a scanning mutagenesis approach to identify residues in th

75 In this work, we have used an alanine scanning mutagenesis approach to identify whether both c

76 Here, we use a systematic alanine scanning mutagenesis approach to understand the role of

77 ins at this interface, identified by alanine scanning mutagenesis, are conserved among DSX homologs.

78 ese differences, we employed a "phylogenetic scanning mutagenesis" assay and identified a cluster of

79 the in vivo role of the linker using alanine scanning mutagenesis, assaying stressosome output in B.

80 it interactions of K33, we performed alanine-scanning mutagenesis at charged residues in the membrane

81 rom loss-of-binding studies using an alanine scanning mutagenesis-based epitope mapping approach.

82 logous urea binding site, horizontal alanine scanning mutagenesis between p51 residues Lys-275 and Th

83 er tested the relevance of this model by Ala-scanning mutagenesis, but only one of five substitutions

84 We performed extensive scanning mutagenesis by changing every amino acid residu

85 r variant previously stabilized by extensive scanning mutagenesis) by more than 30 degrees C and 11 d

86 The use of a panel of agonists and scanning mutagenesis can define the critical binding reg

87 grammes for performing computational alanine-scanning mutagenesis (CASM) to guide experiments.

88 Here we show that Trp scanning mutagenesis combined with fluorescence spectros

89 Alanine scanning mutagenesis confirms that these residues are im

90 Here, we use scanning mutagenesis coupled with reporter assays, bioch

91 RON2L1-bound forms complemented with alanine scanning mutagenesis data reveal an unexpected architect

92 Based on the scanning mutagenesis data, refined models, and additiona

93 channels, GLIC and ELIC, was examined by Ala scanning mutagenesis, deletion mutations, and mutant cyc

94 Alanine scanning mutagenesis demonstrated that positively charge

95 Scanning mutagenesis demonstrates that basic and hydroph

96 Alanine scanning mutagenesis determined that the key residues in

97 Using a combined approach of scanning mutagenesis, electrophysiology, chemical ligand

98 beta-sheets, we performed extensive alanine-scanning mutagenesis experiments on the single-layer bet

99 Fine-scale deletion and scanning mutagenesis experiments with 1 tandem repeat un

100 Here, using topology predictors, cysteine-scanning mutagenesis, expression of GFP-tagged protein v

101 to homology models of hERG1 corroborated the scanning mutagenesis findings.

102 during activation were analyzed by cysteine-scanning mutagenesis followed by disulfide cross-linking

103 Isolated A2 was subjected to alanine-scanning mutagenesis followed by expression of a set of

104 Thus phylogeny-based scanning mutagenesis has allowed us to identify novel ro

105 of both peptides, generated using positional scanning mutagenesis, have decreased trypsin affinity, w

106 ificity of antibodies was studied by homolog-scanning mutagenesis (HSM) with single human domain huma

107 Alanine-scanning mutagenesis identified a functional TILRR mutan

108 Electron microscopy combined with scanning mutagenesis identified ab8 interactions with al

109 Alanine-scanning mutagenesis identified an acidic-residue putati

110 Ala scanning mutagenesis identified mutants defective in sub

111 Using 2C6 and variants, alanine scanning mutagenesis identified three amino acids (aa 59

112 tative translocation pathway, using cysteine-scanning mutagenesis in conjunction with solvent-accessi

113 ructural model, was investigated by cysteine-scanning mutagenesis in conjunction with the substituted

114 Alanine scanning mutagenesis in sGC indicates that the H-NOXA do

115 Using alanine scanning mutagenesis in the activity subdomain of RID(Vc

116 lowing this discovery, we performed cysteine-scanning mutagenesis in the EpoR juxtamembrane and trans

117 Here, by using histidine scanning mutagenesis in the Shaker K channel, we identif

118 Using homology-scanning mutagenesis in which domains of GLUT1 are subst

119 Using homology-scanning mutagenesis in which GLUT1 domains are substitu

120 Using alanine-scanning mutagenesis, in cellulo bioluminescence resonan

121 diversity of novel insertions by insertional-scanning mutagenesis (InScaM).

122 In experimental protein engineering, alanine-scanning mutagenesis involves the replacement of selecte

123 Combinatorial scanning mutagenesis libraries were prepared in which CD

124 Here, scanning mutagenesis located a tryptophan-tolerant regio

125 Using alanine-scanning mutagenesis, loss-of-function recombinant prote

126 Alanine scanning mutagenesis maps a novel epitope to a shallow g

127 ract and bind alpha2-AP, and used an alanine-scanning mutagenesis method to select residues having hi

128 Alanine scanning mutagenesis of 12 Arg/Lys residues of exosite 2

129 We now use cysteine-scanning mutagenesis of 14 TM IV residues, bioluminescen

130 We have performed surface acidic-to-alanine-scanning mutagenesis of 3C to identify the surface of 3C

131 Alanine-scanning mutagenesis of 48 amino acids combined with enz

132 Ala-scanning mutagenesis of 97 residues covering 53% of the

133 s, based on the knowledge available from Cys-scanning mutagenesis of a prototypic homolog.

134 In this report we have used alanine-scanning mutagenesis of a putative coiled coil at the C-

135 We thus performed extensive alanine scanning mutagenesis of A1AR-ECL2 to explore the role of

136 We used positional scanning mutagenesis of alpha-conotoxin PeIA, which targ

137 the first complete charge-cluster-to-alanine scanning mutagenesis of an ARP and compared the results

138 Alanine scanning mutagenesis of areas flanking Leu(172), Thr(281

139 In this work, we performed alanine scanning mutagenesis of aromatic residues located in tra

140 We performed alanine scanning mutagenesis of AuIB and alpha3beta4 nAChR, homo

141 Alanine-scanning mutagenesis of B. subtilis alphaCTD uncovered r

142 o test this possibility, we employed alanine scanning mutagenesis of CB1 EC2 and identified two disti

143 Here, using a 2-electrode voltage clamp and scanning mutagenesis of channels heterologously expresse

144 rents elicited by cystine and neutralization-scanning mutagenesis of conserved protonatable residues,

145 Charged-to-alanine scanning mutagenesis of CSM4 yielded one allele, csm4-3,

146 Alanine-scanning mutagenesis of DC-SIGN revealed that highly con

147 idues of claudin-2 by comprehensive cysteine-scanning mutagenesis of ECL1.

148 Alanine-scanning mutagenesis of FNR amino acid residues 181 to 1

149 Here, we used scanning mutagenesis of hERG1 to identify the molecular

150 Alanine scanning mutagenesis of hVbeta2.1 wild-type and mutated

151 Alanine-scanning mutagenesis of HVEM was used to further define

152 Results from alanine-scanning mutagenesis of hydrophobic residues in the N te

153 nd receptor activation, we performed alanine scanning mutagenesis of loop residues and assessed the i

154 Using alanine scanning mutagenesis of MBM1, we found that the hydropho

155 Alanine scanning mutagenesis of MPER indicates that it has a reg

156 Alanine-scanning mutagenesis of predicted catalytic residues sho

157 Scanning mutagenesis of putative residues responsible fo

158 Alanine-scanning mutagenesis of Qtip shows that its localization

159 tor activity were assessed following alanine scanning mutagenesis of residues 555-571 that border or

160 Alanine scanning mutagenesis of residues 64-70, within full-leng

161 Scanning mutagenesis of residues in the region preceding

162 f Escherichia coli, by an extensive cysteine scanning mutagenesis of residues likely involved in liga

163 Alanine-scanning mutagenesis of residues predicted to line a tra

164 TCR, complemented with computational alanine-scanning mutagenesis of SEA, SEB, SEC3, SEE, and SEH.

165 Using alanine-scanning mutagenesis of sigma32 and in vivo and in vitro

166 other plant species and a saturating NAAIRS-scanning mutagenesis of SNI1 identified regions in SNI1

167 ues to CNTO607 binding, we performed alanine-scanning mutagenesis of the A-D region of IL-13.

168 We combined alanine-scanning mutagenesis of the A1AR second extracellular lo

169 Alanine scanning mutagenesis of the alpha-region reveals that fi

170 Alanine-scanning mutagenesis of the alternatively spliced AT-hoo

171 Alanine scanning mutagenesis of the C-terminal region of TnrA id

172 Alanine scanning mutagenesis of the conserved residues of this l

173 In this study, we performed alanine scanning mutagenesis of the ExsA alpha-helix (residues 1

174 eras between these OCLN proteins and alanine scanning mutagenesis of the extracellular domains of OCL

175 Alanine-scanning mutagenesis of the intracellular C terminus ide

176 Alanine scanning mutagenesis of the LIM interaction domain of LD

177 However, tryptophan and cysteine scanning mutagenesis of the M4 segment, as well as recov

178 Further, scanning mutagenesis of the N terminus of Kvbeta1.3 reve

179 Alanine-scanning mutagenesis of the Na(v)1.6 N terminus demonstr

180 inant mutant CSFVs was created using alanine scanning mutagenesis of the p7 gene harboring sequential

181 iochemical mechanism by carrying out alanine-scanning mutagenesis of the PKR activation domain of PAC

182 Ala-scanning mutagenesis of the pore domain of Kv1.5 identif

183 Proline-scanning mutagenesis of the predicted helix revealed tha

184 Scanning mutagenesis of the SH2-pseudokinase domain link

185 mutagenesis results, we performed an alanine scanning mutagenesis of the TLR2 DD loop and part of the

186 Scanning mutagenesis of the two TMs identifies residues

187 with the membrane, we carried out a cysteine-scanning mutagenesis of the whole motif M240-K257.

188 role in recognition, we carried out homolog-scanning mutagenesis of the zinc-binding domain of DNA p

189 In the present study, we show by Ala-scanning mutagenesis of the ZPI-binding interface, toget

190 Alanine-scanning mutagenesis of these nine side chains shows tha

191 We performed alanine-scanning mutagenesis of this motif ((14)TFPLF(18)) in Um

192 Alanine scanning mutagenesis of this region revealed that a sing

193 Alanine-scanning mutagenesis of this region suggested that repla

194 We performed alanine scanning mutagenesis of this region, and we quantified i

195 Alanine scanning mutagenesis of three to five consecutive cytopl

196 ay of ion conductance, we performed cysteine scanning mutagenesis of transmembrane domain three follo

197 al role of this region, we performed "random scanning mutagenesis" of 11 motif B residues and screene

198 Here, we performed comprehensive alanine-scanning mutagenesis on csrA of E. coli and tested the 5

199 Towards this end, we performed alanine scanning mutagenesis on selected residues in the amino t

200 In this report, we conduct alanine-scanning mutagenesis on the 14 other conserved sites usi

201 S4A in these processes, we conducted alanine scanning mutagenesis on the C-terminal acidic domain of

202 ctors that govern MCP assembly by performing scanning mutagenesis on the surface residues of PduA, a

203 This model was used to design cysteine-scanning mutagenesis on transmembrane (TM) segments 3 an

204 120, and its footprint as defined by alanine-scanning mutagenesis overlaps that of b12.

205 resolution structure of APETx2 combined with scanning mutagenesis revealed a cluster of aromatic and

206 Alanine scanning mutagenesis revealed a requirement for the KRRR

207 Alanine-scanning mutagenesis revealed five additional residues i

208 Alanine scanning mutagenesis revealed four classes of mutants: m

209 Alanine scanning mutagenesis revealed that amino acids K54, R55,

210 Alanine-scanning mutagenesis revealed that an F protein with a s

211 Alanine-scanning mutagenesis revealed that residues 1773, 1777,

212 Alanine-scanning mutagenesis revealed that the affinities of the

213 Alanine scanning mutagenesis revealed that the K/R-R/x-x-E/D mot

214 Alanine-scanning mutagenesis revealed the molecular basis of res

215 Scanning mutagenesis reveals that both activators and re

216 with GP, here we used comprehensive alanine-scanning mutagenesis (shotgun mutagenesis), neutralizati

217 Scanning mutagenesis showed that while all contacting Ty

218 In vitro scanning mutagenesis strategies are valuable tools to id

219 ing mutations was generated using an alanine-scanning mutagenesis strategy.

220 Scanning mutagenesis studies have identified key residue

221 Alanine scanning mutagenesis studies revealed that the Thr-35 re

222 Alanine-scanning mutagenesis studies targeting residues in the h

223 270A provides an ideal scaffold for cysteine scanning mutagenesis studies.

224 , L312, A313, and A316, were identified in a scanning mutagenesis study of the BK (Ca(2+)-activated,

225 rformed a comprehensive deletion and alanine scanning mutagenesis study of this protein in the contex

226 d curcumin (CCM), here we perform an alanine scanning mutagenesis study.

227 ntact residues within aa 412 to 423, alanine-scanning mutagenesis suggested that one subset, which in

228 kinase as obtained from full-protein alanine-scanning mutagenesis (systematic mutation) studies, as w

229 he cytoplasm, and with the use of GPS-linker scanning mutagenesis, the C-terminal portion of SadB was

230 imately 300 site-directed mutants by Ala/Leu scanning mutagenesis, the expression of each mutant in m

231 cid transporter were determined via cysteine-scanning mutagenesis, thiol modification, and in silico

232 Following systematic cysteine-scanning mutagenesis, thiol-specific modification of sev

233 Deletion or alanine- scanning mutagenesis through this domain significantly a

234 e structure of ENaC, we performed tryptophan-scanning mutagenesis throughout alphaM1 (residues 110-13

235 Scanning mutagenesis throughout much of the ectodomain f

236 We used scanning mutagenesis throughout the TM5-TM6 linker, a re

237 for Ubc9 was mapped by deletion and alanine-scanning mutagenesis to a consensus motif for SUMOylatio

238 e correlation spectroscopy (FCS) and alanine-scanning mutagenesis to characterize the interactions of

239 (USPs) and used phage display and saturation scanning mutagenesis to comprehensively map functional e

240 t bound Gbetagamma were subjected to alanine scanning mutagenesis to determine their relevance to the

241 In this work, we used alanine-scanning mutagenesis to elucidate the structural basis f

242 We performed cysteine-scanning mutagenesis to examine the role of TMD8 residue

243 In this work we use alanine-scanning mutagenesis to explore the contributions of 29

244 First, we used scanning mutagenesis to identify and compare patterns of

245 We have used structure-directed alanine scanning mutagenesis to identify determinants in these d

246 lyzed by combining triple and single alanine scanning mutagenesis to identify individual residues req

247 We used alanine-scanning mutagenesis to identify regions of human MD-2 t

248 We used NMR experiments and alanine scanning mutagenesis to identify residues in the zinc bi

249 In this study, we used alanine scanning mutagenesis to identify the key residues in thi

250 This study applied alanine-scanning mutagenesis to investigate the role of the comp

251 We then used alanine scanning mutagenesis to locate the epitopes.

252 Herein, we used structure-guided alanine-scanning mutagenesis to map the functional epitope and p

253 To address this question, we used cysteine scanning mutagenesis to monitor aqueous accessibility of

254 We used alanine scanning mutagenesis to replace selected amino acids in

255 estigate this hypothesis, we applied Ala/Val scanning mutagenesis to the S4-S5 linker and the post-PV

256 The current study used alanine scanning mutagenesis to understand the selectivity profi

257 Here, we utilized "homology scanning" mutagenesis to identify beta tail mutants sele

258 om peptide phage display mapping and alanine scanning mutagenesis, to identify residues in the HCV E2

259 First, 32 alanine-scanning mutagenesis variants of dystrophin R16-17 indic

260 Alanine-scanning mutagenesis was applied to the corresponding Ar

261 mportant for Ste2p function, so a systematic scanning mutagenesis was carried out in which 46 residue

262 entered around the sequence "SQELD." Alanine scanning mutagenesis was carried out on the T(35)IXXSQ E

263 Alanine scanning mutagenesis was performed for the residues of 5

264 Systematic alanine scanning mutagenesis was performed on the substrate-bind

265 Alanine scanning mutagenesis was performed to assess the roles o

266 fluenza virus 5 F protein TM domain, alanine scanning mutagenesis was performed.

267 Alanine-scanning mutagenesis was previously performed to identif

268 Alanine-scanning mutagenesis was previously performed to identif

269 Cys-scanning mutagenesis was then performed in which individ

270 In this study, alanine scanning mutagenesis was used in combination with determ

271 Alanine-scanning mutagenesis was used to confirm that the newly

272 Alanine scanning mutagenesis was used to determine the role of e

273 Here, scanning mutagenesis was used to examine the effects of

274 In this study, charged to alanine-scanning mutagenesis was used to generate conditional-le

275 of Dbp5 in Saccharomyces cerevisiae, alanine scanning mutagenesis was used to generate point mutants

276 Alanine-scanning mutagenesis was used to identify YscF mutants t

277 Transposon-mediated saturation linker scanning mutagenesis was used to isolate fully replicati

278 Alanine scanning mutagenesis was used to map the specific amino

279 oQ in the physiological homodimer, disulfide-scanning mutagenesis was used.

280 Using alanine-scanning mutagenesis we found four residues, all located

281 Through extensive deletion and alanine-scanning mutagenesis we have mapped key residues for int

282 By performing alanine-scanning mutagenesis we identified a dilysine sequence (

283 Here, by using scanning mutagenesis we identify critical residues in hu

284 By scanning mutagenesis, we define individual amino acids r

285 Using alanine scanning mutagenesis, we demonstrate that the binding si

286 Using alanine-scanning mutagenesis, we have made mutations in 79 of 31

287 Using linker scanning mutagenesis, we have narrowed down minimal Ter1

288 Using alanine-scanning mutagenesis, we identified two clusters of resi

289 Using alanine-scanning mutagenesis, we probed the roles of two active-

290 Using alanine scanning mutagenesis, we show that a pore helix residue

291 Using alanine scanning mutagenesis, we show that two distinct regions

292 The results obtained using Trp scanning mutagenesis were confirmed using Cys residues l

293 es using peptide phage-display libraries and scanning mutagenesis, which suggested a significantly en

294 Here scanning mutagenesis with alanine and proline was perfor

295 s of ICMT critical for activity, we combined scanning mutagenesis with methyltransferase assays.

296 We have performed cysteine-scanning mutagenesis with serine or valine replacement t

297 has been systematically examined by alanine scanning mutagenesis, with subsequent characterization o

298 We next performed Cys-scanning mutagenesis within the region of amino acids 35

299 Alanine scanning mutagenesis within the region of amino acids 50

300 d of free Cys as the template to perform Cys-scanning mutagenesis within these regions.