ライフサイエンスコーパス: secondary antibody

1 crosslinking veltuzumab or rituximab with a secondary antibody.

2 e cytotoxin saporin either directly or via a secondary antibody.

3 body fragments followed by an anti-human IgG secondary antibody.

4 lonal anti-BrdU antibody and FITC-conjugated secondary antibody.

5 s performed using glucose oxidase-conjugated secondary antibody.

6 orescent label was located on the primary or secondary antibody.

7 onstrated by complexing the Au particle to a secondary antibody.

8 -ir by using a Bodipy fluorophore conjugated secondary antibody.

9 diaminobenzidine and a peroxidase-conjugated secondary antibody.

10 with and anti-TrkA antibody and fluorescent secondary antibody.

11 l antibody to BrdU and a fluorescent-labeled secondary antibody.

12 sing a commercially available antimouse IgG1 secondary antibody.

13 ing the antifumonisin antibody and a labeled secondary antibody.

14 mesenchymal antigens as well as a dye-linked secondary antibody.

15 el via reaction with a suitable biotinylated secondary antibody.

16 eradish peroxidase (HRP-labeled anti-PSA) as secondary antibody.

17 ignal generated via a fluorescently labelled secondary antibody.

18 ntibodies and then with a fluorescent-tagged secondary antibody.

19 olled to prevent detection of mouse IgG by a secondary antibody.

20 noclonal antibody through interaction with a secondary antibody.

21 ion of coating antigen, primary antibody and secondary antibody.

22 measurements of attached DyLight649-labeled secondary antibody.

23 evelation using alkaline phosphatase-labeled secondary antibody.

24 e alkynes on ODFs with azide groups added to secondary antibodies.

25 proteins IL-6 and IL-8 using biotin-labeled secondary antibodies.

26 he hydroxyl-terminated nanocrystals with the secondary antibodies.

27 AM-1) antibodies followed by FITC-conjugated secondary antibodies.

28 t was used to separate fluorescently labeled secondary antibodies.

29 ith diaminobenzidine or by using fluorescent secondary antibodies.

30 yanine-conjugated and fluorescein-conjugated secondary antibodies.

31 of receptor and phosphotyrosine content with secondary antibodies.

32 yanine-conjugated and fluorescein-conjugated secondary antibodies.

33 ated because of cross-reactivity between the secondary antibodies.

34 be quantitatively differentiated by adopting secondary antibodies.

35 smaller label displacement than traditional secondary antibodies.

36 fotyrosine primary and Cy3-labeled antimouse secondary antibodies.

37 jugate as well as affinity bound primary and secondary antibodies.

38 horseradish peroxidase (HRP) labeled on the secondary antibodies.

39 ed by a cocktail of fluorescently conjugated secondary antibodies.

40 by the immunoreaction with the biotinylated secondary antibodies.

41 ody ESH8 followed by a phycoerythrin-labeled secondary antibody; (2) biotinylated C1C2 detected by ph

42 F4 monoclonal antibody and an anti-mouse IgG secondary antibody, a protease-resistant PrP was reporte

43 Secondary antibodies (Ab(2)) attached to carboxylated mu

44 Measurements employed goat antichicken secondary antibodies (Ab(2)) labeled with horseradish pe

45 ring horseradish peroxidase (HRP) labels and secondary antibodies (Ab(2)) linked to carbon nanotubes

46 Biotinylated secondary antibodies (Ab(2)) that bind specifically to s

47 ased on a competitive inhibition format with secondary antibodies (Ab2) conjugated to gold nanopartic

48 tilized the L@MIL-53(Fe)-NH(2) conjugated to secondary antibody (Ab2) as a signaling probe and coreac

49 otinylated-horseradish peroxidase-conjugated secondary antibody, after which sections were reacted wi

50 and unprocessed whole blood samples by using secondary antibodies against human IgE labeled with 40 n

51 enhance the sensor response to biotinylated secondary antibody against CEA.

52 ody targeting DNA-RNA hybrids and polyclonal secondary antibody, all performed without washing steps.

53 ing capacity (ABC) of a cell population, the secondary antibody amplification (psi), the particle-mag

54 concentration of 10(7)spores/mL, and with a secondary antibody amplification at a concentration of 1

55 After secondary antibody amplification, reactions were visuali

56 equal concentrations (10(7)spores/mL) with a secondary antibody amplification.

57 tected either by using peroxidase conjugated secondary antibodies and developing with diaminobenzidin

58 ndex [IBI], >=2.5) using multiple anti-human secondary antibodies and end-titers were calculated if s

59 l antibodies (MAbs) and fluorescently tagged secondary antibodies and examined by confocal microscopy

60 or pathogen detection that avoids the use of secondary antibodies and is revealed by the photolumines

61 re still limited owing to the requirement of secondary antibodies and negatively charged polymers, an

62 ity was controlled by omission of primary or secondary antibodies and pre-absorption test.

63 e bound bacterial toxins with a biotinylated secondary antibodies and streptavidin-coated MB resulted

64 tting with quantitation using [125I]-labeled secondary antibody and a phosphorimager.

65 lian Na-K-Cl cotransporter and a fluorescent secondary antibody and examined under a fluorescent micr

66 labeled with horseradish peroxidase (HRP) as secondary antibody and H(2)O(2) as the substrate.

67 hworks were treated with a rhodamine-labeled secondary antibody and sectioned, and the number of inge

68 d with labeled antibodies) or incubated with secondary antibody and then flat mounted (retinas from e

69 Glucose oxidase was labeled to the secondary antibody and yielded hydrogen peroxide that ac

70 y antibodies and their corresponding labeled secondary antibodies, and surface-enhanced Raman scatter

71 s against complement components, fluorescent secondary antibodies, and the analysis of >150 images to

72 enzymatically labeled with an HRP-conjugated secondary antibody, and amperometric transduction was pe

73 A secondary antibody (Aptamer-Antibody Assay) or a lectin

74 In general immunoassays, secondary antibodies are covalently linked with enzymes

75 Polyclonal anti-immunoglobulin G (anti-IgG) secondary antibodies are essential tools for many molecu

76 The secondary antibody arm (antibody 679) recognizes a hista

77 pecific receptor molecule and the QD-labeled secondary antibody as a dual (fluorescence cum electroch

78 ein G for conjugation and orientation of the secondary antibody as signal labels.

79 PTHrPs are bound to a secondary antibody attached to a polyhorseradish peroxid

80 electrochemically detectable gold-conjugated secondary antibody (Au-2 degrees Ab).

81 dwiching afterwards with AuNPs modified with secondary antibodies (AuNPs-sECAb) and detected using ch

82 Sensor responses were confirmed using a secondary antibody binding step, similar to the sandwich

83 e cytotoxin saporin either directly or via a secondary antibody, both resulted in prostate cancer cel

84 Imaging secondary antibodies bound to M1-AQP4 allowed us to infe

85 After flushing away excess reagents, secondary antibodies bound to their targets are then det

86 Our system not only eliminates secondary antibodies but also serves as a novel method p

87 nium ester as the chemiluminescent probe and secondary antibody-coated paramagnetic particles for the

88 ellular compartment with fluorescent primary-secondary antibody complexes, total internal reflection

89 competitive immunoassay that is amplified by secondary antibodies conjugated with Au nanoparticles.

90 t the virus env surface protein, followed by secondary antibodies conjugated with colloidal gold part

91 ntibodies specific to the heteroduplexes and secondary antibodies conjugated with HRP onto the surfac

92 Subsequent rinsing, binding of a secondary antibody conjugated to a fluorophore, and a se

93 ke amplification strategy was set up using a secondary antibody conjugated to horseradish peroxidase

94 n anti-LfR antibody that was detected with a secondary antibody conjugated with a red-color fluoresce

95 ssed using a specific primary antibody and a secondary antibody conjugated with Alexa 488 fluorescent

96 fter washing, the sections were treated with secondary antibody conjugated with FITC.

97 proteolysis of VAMP2 as a marked decrease in secondary antibody-conjugated colloidal gold particles t

98 and neuraminidase, coupled with appropriate secondary antibody conjugates.

99 nt HEV infection in patients with primary or secondary antibody deficiency.

100 nt add further evidence linking clozapine to secondary antibody deficiency.

101 the basis for a mechanistic understanding of secondary antibody diversification and the subsequent ge

102 antigen-specific B cells undergo a phase of secondary antibody diversification in germinal centers (

103 subsequent binding of a fluorophore-labeled secondary antibody enables quantitation of the captured

104 (1 microL for the antigen, 1 microL for the secondary antibody-enzyme conjugate, and 200 nL for the

105 and specificity, followed by binding with a secondary antibody-enzyme conjugate.

106 rescein-labeled antibodies and enzyme-linked secondary antibodies for quantification of purified CTB.

107 CDK phosphorylation, and a Europium-labeled secondary antibody for signal detection.

108 NTs are modified and then decorated with the secondary antibody for tau protein.

109 ong-lived sources of rapid, isotype-switched secondary antibody-forming cell (AFC) responses.

110 toreactivity, yet, in an autoimmune context, secondary antibody gene rearrangements might also contri

111 specific antibodies followed by binding of a secondary antibody horseradish peroxidase (HRP) complex

112 s (CNSs) labeled with horseradish peroxidase-secondary antibodies (HRP-Ab2).

113 d with a mixture of conventional HRP-labeled secondary antibodies (HRP-anti-human IgG/IgM/IgA mixture

114 modify the binding of subsequent primary or secondary antibodies; (ii) the IgM MAbs bind primarily t

115 agnetic beads and incubated with primary and secondary antibodies in bulk and then arrayed in a 96-we

116 inity-purified antibodies ordinarily used as secondary antibodies in immunodetection protocols were b

117 ochemical methods using a peroxidase-labeled secondary antibody in albino mice revealed heavy labelin

118 sters is detectable using the anti-mouse IgG secondary antibody in the absence of the 3F4 monoclonal

119 xidase (HRP) was used as label on detection (secondary) antibodies in a sandwich immunoassay scheme.

120 ycoerythrin), and goat anti-mouse polyclonal secondary antibody (indodicarbocyanin).

121 apical PM was confirmed by microinjection of secondary antibodies into the bile canalicular-like spac

122 The amount of the bound secondary antibody is directly proportional to the conce

123 Secondary antibodies labeled with conventional organic d

124 ike RBD and human ACE2, and its detection by secondary antibodies labeled with NanoLuc luciferase fra

125 Secondary antibodies labeled with two spectrally distinc

126 detection assay of GNPs-antiENaC bound to a secondary antibody labeled with a fluorophore.

127 A secondary antibody labeled with dyes/quantum dots (QDs)

128 PCE, where tau protein was sandwiched with a secondary antibody labeled with horseradish peroxidase (

129 rmed with electron microscopy and the use of secondary antibody labeled with horseradish peroxidase.

130 ated emission depletion nanoscopy, we imaged secondary antibody labeling of monoclonal AQP4-IgGs with

131 ell, (2) primary antibody labeling well, (3) secondary antibody labeling well, and (4) readout buffer

132 , respectively, compared to the conventional secondary antibody labeling.

133 clusters, and estimate the average number of secondary antibody labels per cluster.

134 no mass amplification and with sandwich-type secondary antibody mass amplification.

135 ube (SWNT) forest platforms with multi-label secondary antibody-nanotube bioconjugates for highly sen

136 w that antibody cross-linking (anti-MOG plus secondary antibody) of MOG on the surface of OLs results

137 NA whose outer-layer arms were hybridized to secondary antibody-oligonucleotide conjugates.

138 ody and Horse Radish Peroxidase (HRP) tagged secondary antibody on the surface of GCE/f-MWCNT-Chit@Th

139 and horseradish peroxidase (HRP)-conjugated secondary antibody onto AgNPs-rGO modified-SPEs to fabri

140 cept, the coupling of enzymatic reporters to secondary antibodies or antigens often results in low yi

141 ompetitive immunoassays without the need for secondary antibodies or further labeling.

142 ather lengthy and involve the use of labeled secondary antibodies or other agents to provide a signal

143 ng the antibody to the beads with Protein L, secondary antibody, or streptavidin: the high-stability

144 ive immunoassay format without the need of a secondary antibody, or washing steps.

145 through of primary and peroxidase-conjugated secondary antibodies over the course of 3 min enhanced p

146 Cross-linking with secondary antibody overcame the inhibitory effect of ant

147 gnal response from label-free, sandwich with secondary antibody (pAb), and pAb functionalized with ir

148 icted by the limited availability of primary/secondary antibody pairs.

149 luorescent probe, covalently attached to the secondary antibody, plays a crucial role of indicating c

150 Analyte proteins are captured by secondary antibody-poly-HPR (horseradish peroxidase) bio

151 Unexpectedly, the anti-FLAG secondary antibody produced positive results with CaLas

152 me epitopes combined with a goat anti-rabbit secondary antibody produced very strong purple color in

153 cale in Escherichia coli and could thus make secondary antibody production in animals obsolete.

154 say where an alkaline phosphatase conjugated secondary antibody reacts with p-aminophenyl phosphate (

155 commercially available and species-specific secondary antibody reagents.

156 nventional horseradish-peroxidase-conjugated secondary antibodies, regardless of the target molecules

157 genes, which underlie the generation of the secondary antibody repertoire in B lymphocytes.

158 sfully primed the mice to mount an effective secondary antibody response after a single boost with TB

159 ulates the target epitope specificity of the secondary antibody response and has wide-reaching conseq

160 (FH)) cells play an essential role to induce secondary antibody response by providing help to memory

161 y than IgM among ZIKV patients, suggesting a secondary antibody response to assay antigens following

162 Secondary antibody response to rechallenge in passively

163 cterial numbers in a manner reminiscent of a secondary antibody response to rechallenge.

164 intravenous injection and does not induce a secondary antibody response when given to mice previousl

165 3d with low C3d/PPS14 ratios had an enhanced secondary antibody response.

166 al boosting, generated a strong long-lasting secondary antibody response.

167 centers and a detectable, although reduced, secondary antibody response.

168 rized to hFVIII generated normal primary and secondary antibody responses after immunization with the

169 reviously vaccinated participants manifested secondary antibody responses after receipt of low-dose A

170 y primary IgM antibody responses followed by secondary antibody responses associated with immune memo

171 ed the induction of long-lasting primary and secondary antibody responses post-RABV immunization.IMPO

172 lder avian influenza H5 strain might lead to secondary antibody responses to a single dose of more cu

173 can have distinct effects on the primary and secondary antibody responses to a T-cell-independent typ

174 We now show that TCI induces secondary antibody responses to coadministered antigens

175 uring efalizumab treatment, both primary and secondary antibody responses to phiX174, including IgM/I

176 Some studies also described secondary antibody responses upon exposure.

177 influenza but failed to generate protective secondary antibody responses when challenged with influe

178 survival, (2) inhibition of primary but not secondary antibody responses, and (3) minimal drug toxic

179 tolerized mice exhibited normal primary and secondary antibody responses, suggesting that transient

180 line precursors, account for the majority of secondary antibody responses, while most primary-derived

181 lammatory context is required to induce B-1a secondary antibody responses.

182 anoparticles containing [Ru(bpy)(3)](2+) and secondary antibodies (RuBPY-silica-Ab(2)) are employed i

183 AM1 antibody and then incubated with labeled secondary antibody showed selective staining of retinal

184 ecognition event was displayed using labeled secondary antibody solution and subsequent signal amplif

185 as a nano-dispersed label were conjugated to secondary antibodies specific to anti-porcine IgG.

186 dingly through a multiplex immunoassay using secondary antibodies specific to each patterned primary

187 "anchors," which were functionalized with a secondary antibody specific to the Fc region of the prim

188 osensor horseradish peroxidase (HRP) labeled secondary antibodies, specifically interacting with the

189 Immunogold and alkaline phosphatase secondary antibody staining both show antigen in seconda

190 RS staining (unlabelled primary and labelled secondary antibody) techniques were employed.

191 edly labeling target proteins with a pair of secondary antibodies that bind to each other.

192 ecognition domains and recognition sites for secondary antibodies that can decrease the signal of imm

193 a recombinant K39 antigen and detected with secondary antibodies that were conjugated with cleavable

194 In the presence of secondary antibodies the analytical sensitivity was impr

195 iomolecular recognition of these branches by secondary antibodies, the 'extensions' on the cytoskelet

196 d with an anti-gp75 antibody and fluorescent secondary antibody, the cells are then fixed to prevent

197 fic antibodies and then with gold-conjugated secondary antibodies; the thin sections were examined by

198 shes survival of germinal center B cells and secondary antibody titers.

199 The assay uses infrared-labeled secondary antibodies to detect phospho-ERK, and the sign

200 GFAP and detected with fluorescently labeled secondary antibodies to quantify co-localization with th

201 ed with horseradish peroxidase (HRP) labeled secondary antibodies to the trans-membrane protein CD44.

202 By employing a more massive secondary antibody to amplify the signal arising from th

203 with the Ru-silica@Au nanocomposite labeled secondary antibody to form a sandwich-type immunocomplex

204 d CD31 and LYVE-1 followed by Cy3-conjugated secondary antibody to quantify corneal blood and lymphat

205 A or 20 min by ICGA without species-specific secondary antibodies under field conditions, thus allowi

206 Next, we show that all secondary antibodies used in FRACTAL need to be mutually

207 and a fluorescein-isothiocyanate-conjugated secondary antibody was performed on cryostat sections of

208 lubilized, and then immunoprecipitation with secondary antibody was performed.

209 abbit anti-iNOS antibody was employed as the secondary antibody, was developed.

210 s amplification of gold-nanoparticle labeled secondary antibodies, we establish a detection limit of

211 antibodies bound to the surface-immobilized secondary antibodies, we observed this binding via chang

212 Then, the peroxidase-labeled secondary antibodies were added to bind these primary an

213 Afterward, fluorescein-conjugated secondary antibodies were applied to the wire for quanti

214 tached), and alkaline phosphatase-conjugated secondary antibodies were bound to the analytes attached

215 Secondary antibodies were fluorescein isothiocyanate-con

216 Secondary antibodies were fluorescein-conjugated anti-mo

217 in was placed on test line; species specific secondary antibodies were placed on the control line of

218 Multiple ODF-tagged secondary antibodies were then used to mark primary anti

219 Selected sets of the differently labeled secondary antibodies were then used to simultaneously ma

220 Protein immune detection requires secondary antibodies which must be carefully selected in

221 e conventional IHC process using dye-labeled secondary antibodies (which already has a built-in signa

222 captured analytes labeled with biotinylated secondary antibodies, which then capture streptavidin-po

223 A secondary antibody, which was specific to the adenovirus

224 ti-5'-bromo-2'-deoxyuridine antibodies using secondary antibodies with red and green fluorescence, re

225 rget miRNA was accomplished by labeling such secondary antibodies with the horseradish peroxidase (HR

226 o the immunochemical complex by labeling the secondary antibody with a magnetically susceptible, micr

227 beads as support of immunological chain and secondary antibody with alkaline phosphatase as immunolo

228 ng) or using a more robust reporter (e.g., a secondary antibody with biotin-streptavidin).