ライフサイエンスコーパス: secretin

1 of the peripheral structure around the PilQ secretin.

2 as significantly lower and not responsive to secretin.

3 types of large cholangiocytes and respond to secretin.

4 or mediator of FA-induced release of CCK and secretin.

5 shown to interact with the N0 domain of the secretin.

6 of OutC and explore its interaction with the secretin.

7 tase activity and proliferative responses to secretin.

8 cludes glucagon, glucagon-like peptides, and secretin.

9 anion exchanger 2 and AC8, and responded to secretin.

10 enteroendocrine cells expressing the hormone secretin.

11 ith the potential to produce seven different secretins.

12 om the type II and type III secretion system secretins.

13 ead class of outer-membrane portals known as secretins.

14 were used to evaluate the in vitro effect of secretin (100 nM) on proliferation, protein kinase A (PK

15 Normal WT mice were treated with secretin (2.5 nmoles/kg/day by way of osmotic minipumps

16 g CD36 (vs. vector or CD36K/A) released more secretin (3.5- to 4-fold) and CCK (2- to 3-fold), genera

17 eries of 11 truncated and lactam-constrained secretin(5-27) analogues at the prototypic member of thi

18 id administration to CD36 mice released less secretin (-60%) and CCK (-50%) compared with wild-type m

19 Protein kinase A inhibition (H-89) blunted secretin (80%) but not CCK release, which was reduced (5

20 The authors examined the efficacy of secretin, a hormonal agonist for the prototype group B G

21 the postnatal developing period, we analyzed secretin, a neuropeptide, which is expressed significant

22 coined the term 'hormone' with reference to secretin, a substance they found that was produced by th

23 ted, with emphasis on the synchronization of secretin administration and MR image acquisition.

24 gnificantly improved at 2 and 24 hours after secretin administration but not after treatment with pla

25 reatic ADC obtained with DW imaging prior to secretin administration may aid in diagnosis of CP and a

26 assessment of its severity; ADC response to secretin administration may be less useful.

27 C obtained with DW imaging at 3.0 T prior to secretin administration may help diagnose CP; postsecret

28 The technique of secretin administration will be illustrated, with emphas

29 tudies, one before and one after intravenous secretin administration.

30 400 sec/mm(2)) acquired for 15 minutes after secretin administration.

31 To directly explore this, we prepared six secretin analogue probes that simultaneously incorporate

32 Only Cys(2) and Cys(5) secretin analogues exhibited full activity and retained

33 itions 2, 15, 20, 24, and 25 of full agonist secretin analogues.

34 gspE, T2SS genes encoding an outer membrane secretin and a cytoplasmic ATPase, respectively.

35 patial approximation between residue five of secretin and a residue within the proposed third extrace

36 choleresis and cAMP production stimulated by secretin and acetylcholine, but not by forskolin.

37 Likewise, diminished secretin and CCK responses to FA were observed with CD36

38 ion of the enteroendocrine cell markers CCK, secretin and glucagon while expression of a pan-intestin

39 at under basal conditions and in response to secretin and hypotonicity, cysts from PCK rats expanded

40 continuous coupling interface between the OM secretin and IM rings, which is remarkably facilitated b

41 mong residues in the first five positions of secretin and in every position within the receptor extra

42 Immunoreactive secretin and its mRNA were predominantly found in the tu

43 HCO(3) (-) excretion after stimulation with secretin and often present with metabolic alkalosis.

44 ngiocytes secrete bicarbonate in response to secretin and proliferate after bile duct ligation by act

45 ation similar to that of natural full-length secretin and provided insights into why this peptide was

46 rge-charge interactions between this part of secretin and receptor residues in TM5, TM6, ECL2, and EC

47 Numerous polar interactions formed between secretin and the receptor extracellular loops (ECLs) and

48 rt that concurrent mutation of both the T4bP secretin and the retraction ATPase can result in viable

49 Both secretin and vasoactive intestinal polypeptide (VIP) rec

50 patient's tumor tissue was investigated for secretin and VIP.

51 rotein did not bind or signal in response to secretin and was secreted from transfected MiaPaCa-2 cel

52 dynamic function of type II secretion system secretins and challenge recent studies reporting monomer

53 Cyclic AMP was increased using forskolin or secretin, and Ca(2+) was increased using acetylcholine (

54 protein-coupled receptors (beta-adrenergic, secretin, and cholecystokinin) induces translocation of

55 ase chain reaction, the expression levels of secretin, and VIP were measured.

56 are widely conserved, including NTPases and secretins, and on proteins that are system specific.

57 36 was immunodetected on apical membranes of secretin- and CCK-positive EECs and colocalized with cyt

58 Secretins are bacterial outer membrane proteins that are

59 Secretins are major components of type II and III secret

60 l transformation machineries, outer membrane secretins are suggested to form a multimeric pore for th

61 However, why secretins are toxic to psp null strains, whereas some ot

62 Secretins are versatile outer membrane pores used by man

63 yndrome (WDS) and achlorhydria and establish secretin as a diarrheogenic hormone.

64 Here, we report a new function of secretin as a survival factor for neural progenitor cell

65 ides strong support for the potential use of secretin as a therapy for ductopenic liver diseases.

66 dria in this patient may have been caused by secretin as originally proposed in 1968 and that secreti

67 functional response to the administration of secretin, as depicted on MRCP images, will be illustrate

68 sistent with the notion that the dodecameric secretin assembles as a hexamer of dimers to ensure corr

69 One such protein, which we name T4P secretin-associated protein (TsaP), was identified as a

70 PulC HR domains binding independently at the secretin base.

71 diate cholangiocyte secretion in response to secretin, beta-adrenergic agonists, or changes in [HCO(3

72 The secretin, beta-glucokinase, insulin I, and insulin II ge

73 ied in BECs and contributes to secretion via secretin binding basolateral receptors and increasing [c

74 r oligomerization interface had no effect on secretin binding parameters, it reduced the ability of s

75 constraints were used to refine our model of secretin bound to its receptor, now bringing ECL3 above

76 trom structure, and interrogate dynamics, of secretin bound to the SecR:Gs complex.

77 Nevertheless, some bacteria have secretins but no Psp system.

78 rosis were evaluated as well as secretion of secretin (by cholangiocytes and S cells), expression of

79 These results imply that secretins can direct their own targeting, have complex d

80 xtrudes substrates through an outer membrane secretin channel using a pseudopilus.

81 s the phage through the outer membrane pilus secretin channel.

82 These secretin channels have large periplasmic N-terminal doma

83 id hormone receptor 1 (PTH1R) belongs to the secretin class of G protein coupled receptors (GPCRs) an

84 erium Thermus thermophilus requires a unique secretin complex comprising six stacked rings, a membran

85 The secretin complex of Thermus thermophilus is an oligomer

86 enetically widely conserved component of the secretin complex that co-occurs with genes for T4P in Gr

87 nd suggest that TsaP functions to anchor the secretin complex to the peptidoglycan.

88 on a novel structure of the DNA translocator secretin complex, PilQ, in Thermus thermophilus HB27 com

89 ) through the large, oligomeric, ring-shaped secretin complex.

90 raction of YscD with the outer membrane YscC secretin complex.

91 is activated by mislocalized outer-membrane secretin components of protein export systems and is ess

92 Secretins constitute a superfamily of proteins that asse

93 or by a mutation in the outer-membrane pilus secretin CpaC stimulates early initiation of chromosome

94 inylation of rat cerebellar slices, we found secretin decreased cell-surface Kv1.2 levels by modulati

95 We found that secretin-deficient mice exhibit decreased numbers of Brd

96 ArcB was not required for secretin-dependent induction of psp gene expression.

97 es cholangiocyte growth, direct evidence for secretin-dependent proliferation is lacking.

98 ed to refine our evolving molecular model of secretin docked at the intact receptor, which for the fi

99 Mutations in the secretin domain disrupted the crown and abolished DNA up

100 eriplasmic domain of six stacked rings and a secretin domain in the outer membrane.

101 s and Starling in 1902 of the first hormone, secretin, emerged from earlier observations that a respo

102 Threshold values of non-secretin-enhanced ADC for pancreatitis discrimination we

103 Mean nonenhanced and maximum secretin-enhanced ADCs were higher in patients without C

104 d to define which patients will benefit from secretin-enhanced MR imaging for their treatment plannin

105 Finally, the indications for secretin-enhanced MRCP will be discussed to define which

106 In this review, the technique of secretin-enhanced MRCP, which has the aim to depict the

107 Proteins of the secretin family form large macromolecular complexes, whi

108 Class B1 (secretin family) G protein-coupled receptors (GPCRs) mod

109 Amino-terminal regions of secretin-family peptides contain key determinants for bi

110 s, implying that it forms the outer membrane secretin for PGA.

111 Secretins form multimeric channels across the outer memb

112 similarly independent of the pilin-extruding secretin formed by PilQ and of the hydrophobic-agent eff

113 patic bile duct units after stimulation with secretin, forskolin, HCO(3)(-)/CO(2), cholinergic agonis

114 differences in the architecture of the T4aP secretin from the type II and type III secretion system

115 entified a novel protein required for normal secretin function in P. aeruginosa.

116 s protein is essential for expression of the secretin gene in the murine intestine, and yet it is a r

117 well-supported gene phylogeny of the entire secretin gene superfamily.

118 A secretion-deficient mutant lacking the secretin gene, ysaC, is defective in replication within

119 ynergistically activate transcription of the secretin gene.

120 eptide competition experiments, and chimeric secretin-GLP1 receptor constructs to establish that this

121 Among the vasoactive intestinal peptide/secretin/glucagon family of related peptides, pituitary

122 eptide 1 and 2 (GLP1 and GLP2) belong to the secretin/glucagon/VIP superfamily of peptides and GLP1 a

123 The class B secretin GPCR (SecR) has broad physiological effects, wi

124 The outer membrane secretin, GspD, forms the channels, through which folded

125 Our studies demonstrate that secretin has important implications for neurogenesis in

126 , particularly those for cholecystokinin and secretin, have been better characterized as to the molec

127 Administration of secretin improves noninvasive imaging of the pancreatic

128 The exogenous administration of secretin improves the visualization of pancreatic ducts

129 determine the cryoEM structure of the VcPilQ secretin in amphipol to ~2.7 angstrom.

130 hizophrenia and indicate a possible role for secretin in modulating cerebellar-mediated classically c

131 ular, ExsB promotes the assembly of the T3SS secretin in the bacterial outer membrane, highlighting t

132 n architecture and gene neighborhood of T4aP secretins in Proteobacteria in comparison with VcPilQ.

133 In vitro, secretin increased proliferation, PKA activity, and ERK1

134 Mislocalized secretins induce psp gene expression, and kill psp null

135 In vivo application of secretin induced a marked urinary alkalization, an effec

136 expressed by large cholangiocytes) regulates secretin-induced choleresis.

137 imulated secretion of pancreatic enzymes and secretin-induced gastrointestinal motility, which are me

138 Inter-EEC communication is exemplified by secretin-induced GLP-1 secretion.

139 m after secretin stimulation consistent with secretin-induced secretion.

140 in genes such as PA0943 may cause increased secretin-induced stress to which P. aeruginosa cannot re

141 aeruginosa might deal with the potential for secretin-induced stress without a Psp system.

142 expression and in facilitating tolerance to secretin-induced stress.

143 ivated psp gene expression in the absence of secretin-induced stress.

144 ne expression and to support survival during secretin-induced stress.

145 ned their ability to support survival during secretin-induced stress.

146 These results define the mechanism of secretin-induced urinary HCO(3) (-) excretion, explain m

147 mouse models, we elucidated the mechanism of secretin-induced urinary HCO(3) (-) excretion.

148 Percentage increase in ADC after secretin injection and time to peak ADC did not vary amo

149 (2)) performed serially for 10 minutes after secretin injection.

150 ne our understanding of the structure of the secretin-intact receptor complex and provide new insight

151 Secretin is a hormone that stimulates the exocrine pancr

152 Secretin is a pleiotropic neuropeptide hormone that belo

153 cerebellum and recent findings indicate that secretin is endogenously released in the cerebellum, whe

154 atic gland, induced by the administration of secretin, is described.

155 trointestinal system, it has been found that secretin itself acts as a neuropeptide in the CNS.

156 glucagon receptor (GCGR) are members of the secretin-like class B family of G-protein-coupled recept

157 They suggested a secretin-like hormone of islet cell origin explains WDS

158 rk, we used two high-affinity, full-agonist, secretin-like photolabile probes having sites for covale

159 from ligand associations with rhodopsin- and secretin-like receptors.

160 hodopsin-like), 5 belong to the B-family (or secretin-like), and 2 are leucine-rich repeat-containing

161 etin as originally proposed in 1968 and that secretin may act as a diarrheogenic hormone.

162 This suggests secretin may act in part by suppressing Kv1.2.

163 constitutive homodimers, we explored whether secretin might dock across both protomers of the complex

164 our data suggest that the mechanism by which secretin multimers kill psp null cells is by causing a p

165 Our results indicate that secretin neuropeptidergic signaling is involved in regul

166 These data are consistent with a model of secretin occupying a single secretin receptor protomer w

167 multimeric outer membrane secretion channel (secretin) of the Flp pilus biogenesis apparatus, we obse

168 porting monomers as the basic subunit of the secretin oligomer.

169 effects of vasoactive intestinal peptide and secretin on intra-acinar cell adenosine 3',5'-cyclic mon

170 The effect of secretin on PC excitability is not yet known, but, like

171 2 cells stably expressing CD36 did not alter secretin or CCK release, consistent with a minimal effec

172 rate and interaction with the outer membrane secretin OutD.

173 ence of any transcriptional response to XcpQ secretin overproduction.

174 iated secretion of cholecystokinin (CCK) and secretin, peptides released by enteroendocrine cells (EE

175 The outer membrane secretin PilQ forms a homododecameric ring through which

176 hereby the N-terminal domains of the central secretin PilQ shift by ~30 A, and two periplasmic gates

177 is pilus colocalizes with the outer membrane secretin PilQ.

178 o thread this DNA through the outer membrane secretin, PilQ.

179 amentous phage infection (specifically phage secretin pIV) and by other membrane-damaging agents.

180 -mer InvG, a member of the broadly conserved secretin pore family).

181 s not yet known, but, like Kv1.2 inhibitors, secretin potently increases inhibitory input to PCs.

182 s including gastrin-, glucagon/GLP-1-, CCK-, secretin-producing cell populations and an increase of s

183 becomes dispensable for cholecystokinin and secretin production.

184 ly expressed transcription factor Sp1 to the secretin promoter by NeuroD represents a distinct mechan

185 roteins and facilitates Sp1 occupancy of the secretin promoter in vivo.

186 thway is not required for targeting the BfpB secretin protein of the EPEC T4P to the OM and describe

187 structure has similarities with that of the secretin protein, PilQ, which mediates the transition of

188 gh an outer membrane (OM) pore formed by the secretin protein.

189 itive to the mislocalization of pore-forming secretin proteins.

190 during the mislocalization of outer membrane secretin proteins.

191 during the mislocalization of outer membrane secretin proteins.

192 mbly of one such protein, the outer membrane secretin PulD of the bacterial type II secretion system.

193 embrane-spanning C domain in the dodecameric secretin PulD, the founding member of this class, preven

194 The outer membrane complex includes the secretin PulD, with all domains modelled, and the piloti

195 mulates ductal secretion by interacting with secretin receptor (SR) activating cyclic adenosine 3',5'

196 Activation of the secretin (Sct)/secretin receptor (SR) axis, expressed only by cholangio

197 way and is closely associated with increased secretin receptor (SR) expression.

198 eptide hormone, secretin (SCT) that binds to secretin receptor (SR), is a key mediator in cholangiocy

199 ence complementation to demonstrate that the secretin receptor associates specifically with RAMP3, bu

200 When these peptides probed 61 human secretin receptor cysteine-replacement mutants, a broad

201 on adrenomedullin activity, with increasing secretin receptor expression competing for RAMP3 associa

202 Similarly, secretin receptor expression had functional effects on a

203 As the secretin receptor forms constitutive homodimers, we expl

204 merization of the Class II G protein-coupled secretin receptor has been reported, but the molecular b

205 entified a novel abnormal spliceoform of the secretin receptor in pancreatic and bile duct cancers an

206 the epidermal growth factor receptor and the secretin receptor in the presence and absence of their a

207 onstrate that the agonist occupied wild-type secretin receptor is predominantly in a guanine nucleoti

208 ogical FRET was utilized to demonstrate that secretin receptor oligomerization occurred at the cell s

209 with a model of secretin occupying a single secretin receptor protomer within the homodimeric recept

210 in cancer, we evaluated whether an abnormal secretin receptor spliceoform were present, characterize

211 of exogenous RAMP transfection, although the secretin receptor trafficks normally to the cell surface

212 r specimens and no normal tissue expressed a secretin receptor variant with exons 3 and 4 deleted.

213 gy model of the amino-terminal domain of the secretin receptor was developed using the NMR structure

214 ibrosis transmembrane conductance regulator+/secretin receptor+) and activated phenotype (increased e

215 The secretin receptor, a prototypic family B G protein-coupl

216 ibrosis transmembrane conductance regulator, secretin receptor, and nuclear receptors.

217 ical sodium-dependent bile acid transporter, secretin receptor, cilia and cystic fibrosis transmembra

218 (CD44, CD24, EpCAM, aquaporin 5, claudin-4, secretin receptor, claudin-7, V-ros sarcoma virus oncoge

219 on of PKCbetaII; and (iii) de novo expressed secretin receptor, cystic fibrosis transmembrane regulat

220 specific high-affinity dimeric state of the secretin receptor, which may be typical of family B G pr

221 hly aggressive malignancies that evolve from secretin receptor-rich ductular cells.

222 at the prototypic member of this family, the secretin receptor.

223 abeling a single protomer of the homodimeric secretin receptor.

224 calcitonin receptor with no activity at the secretin receptor.

225 the proposed third extracellular loop of the secretin receptor.

226 (1) secrete bicarbonate by interaction with secretin receptors (SRs) through activation of cystic fi

227 Since secretin receptors activate PKA, we tested the hypothesi

228 beta-Intercalated cells express basolateral secretin receptors and apical CFTR and pendrin.

229 Secretin receptors are also expressed both in PC dendrit

230 expressing wild-type and chimeric calcitonin-secretin receptors.

231 d sequencing of labeled wild-type and mutant secretin receptors.

232 The physiological significance of secretin-regulated Kv1.2 endocytosis is supported by our

233 activate PKA, we tested the hypothesis that secretin regulates Kv1.2 trafficking in the cerebellum.

234 ecretion of the peptides cholecystokinin and secretin, regulation of hepatic lipoprotein output, acti

235 study to investigate whether synthetic human secretin (RG1068)-stimulated MRCP detects pancreatic duc

236 dividuals with schizophrenia received either secretin (RG1068; 20 microg/kg [N=15]) or a saline place

237 erium Neisseria gonorrhoeae, the native PilQ secretin ring embedded in OM sheets is surrounded by an

238 Angiotensin (ANGII) and secretin (SCT) share overlapping, interdependent osmoreg

239 The gastrointestinal peptide hormone, secretin (SCT) that binds to secretin receptor (SR), is

240 Activation of the secretin (Sct)/secretin receptor (SR) axis, expressed on

241 In contrast, the secretin SctC (YscC) and the major export apparatus comp

242 ines replacing each residue in this motif of secretin (sec), Phe(6), Thr(7), and Leu(10), and cystein

243 Complexes of secretin (SecR) and angiotensin 1a (Atr1a) receptors hav

244 The secretin/secretin receptor (SR) axis is up-regulated by

245 Secretin/secretin receptor signaling mediates biliary da

246 roteins from another species, alleviated its secretin sensitivity.

247 em and the TrkA potassium transporter caused secretin sensitivity.

248 ityustoxin-Kalpha, or of the Kv1.2 regulator secretin, significantly enhances acquisition of eyeblink

249 dase activity and physiological responses to secretin, somatostatin and vascular endothelial growth f

250 Significantly higher expression of secretin, SR, and TGF-beta1 was observed in PSC patient

251 Our aim was to determine the role of the secretin/SR axis in activation of biliary fibrosis in an

252 The secretin/SR axis plays a key role in regulating the bili

253 It is not known whether the secretin/SR axis plays a role in subepithelial fibrosis

254 antagonist (Sec 5-27) was used to block the secretin/SR axis.

255 In perfused cortical collecting ducts, secretin stimulated pendrin-dependent Cl(-)/HCO(3) (-) e

256 icipates in intracellular pH homeostasis and secretin-stimulated biliary bicarbonate secretion.

257 SR, CFTR, and Cl(-) /HCO 3- AE2 and ablated secretin-stimulated biliary secretion in these cells.

258 his modulation was functionally biased, with secretin-stimulated calcium responses unaffected, wherea

259 ked down by short hairpin RNA, and basal and secretin-stimulated cAMP levels (a functional index of b

260 ssion of CFTR, Cl(-) /HCO3 (-) AE2, AC8, and secretin-stimulated cAMP levels.

261 ted negative allosteric modulatory impact on secretin-stimulated cAMP responses at SecR.

262 There were no observed changes in secretin-stimulated cAMP, intracellular calcium, ERK1/2

263 Melatonin inhibits biliary growth and secretin-stimulated choleresis in cholestatic bile-duct-

264 cytes and reduced large IBDM; (ii) decreased secretin-stimulated choleresis; and (iii) reduced ERK1/2

265 the percentage of apoptotic cholangiocytes; secretin-stimulated choleresis; and extracellular signal

266 n carriers by pancreatic imaging studies and secretin-stimulated duodenal juice sampling.

267 The secretin-stimulated duodenal juice was studied using cyt

268 cytokines and increased MAPK activity in the secretin-stimulated duodenal juice.

269 (3D)-cultured H69 cholangiocytes blocked the secretin-stimulated expansion of cystic structures devel

270 sulfonic acid disodium salt hydrate) blocked secretin-stimulated fluid accumulation in PCK but not in

271 Corticosteroids increase secretin-stimulated pancreatic bicarbonate concentration

272 assay and performed quantitative imaging of secretin-stimulated pancreatic fluid secretion.

273 Secretin stimulates biliary proliferation by down-regula

274 Secretin stimulates ductal secretion by interacting with

275 activity was observed in the duodenum after secretin stimulation consistent with secretin-induced se

276 (11)C-acetate PET studies with secretin stimulation show potential as a noninvasive met

277 After secretin stimulation testing, the plasma gastrin level r

278 After secretin stimulation, the k1 and k2 significantly increa

279 ricted transcriptional response to prolonged secretin stress in Y. enterocolitica.

280 vocal evidence that PspA is not required for secretin-stress tolerance.

281 f 9 (55%) patients of group 2 had a negative secretin test at hospital discharge.

282 as nonsteroidal anti-inflammatory drugs and secretin, there are currently no universally accepted ag

283 int for docking the amino-terminal region of secretin to its receptor core.

284 In vivo administration of secretin to normal WT mice increased ductal mass.

285 inding parameters, it reduced the ability of secretin to stimulate intracellular cAMP.

286 ich has a novel all-beta-fold, followed by a secretin/TonB, short N-terminal subdomain at the C termi

287 ablished a correlation between the amount of secretin toxicity in a psp null strain and the extent of

288 tion that PspA is not involved in mitigating secretin toxicity.

289 we used sequences of all human rhodopsin and secretin-type G protein-coupled receptors as bait to ret

290 c amylase and fluid secretion in response to secretin, VIP and forskolin through cAMP/PKA pathway act

291 pic neuropeptide hormone that belongs to the secretin/VIP/glucagon peptide family.

292 Secretin was docked to this model using seven sets of sp

293 cture outside the outer membrane capping the secretin was found not to be part of PilQ.

294 The RcpA secretin was necessary for wild-type abundances of RcpB

295 Although secretin was originally isolated in the gastrointestinal

296 Secretin was selected because eye-blink conditioning dep

297 tants, trafficked to the cell surface, where secretin was shown to bind and elicit cAMP production.

298 brane via a large translocation channel, the secretin, which typically adopts a dodecameric structure

299 of the N-terminal periplasmic domain of the secretin XcpQ from Pseudomonas aeruginosa, revealing a t

300 The ring-shaped secretin YscC at the outer membrane was stretched by 30-