ライフサイエンスコーパス: selection marker

1 lsulfatase-encoding ARYLSULFATASE2 gene as a selection marker.

2 of DNA and simultaneously insert a positive selection marker.

3 antibody after silencing a highly expressed selection marker.

4 ce antigen and a gene encoding the kanamycin selection marker.

5 -DNA with the I-SceI site and an integration selection marker.

6 rylase subunit ApL3 were fused to a negative selection marker.

7 tion despite selection for the cotransfected selection marker.

8 ansfected with a separate plasmid encoding a selection marker.

9 ining the cDNA encoding myoD and a puromycin selection marker.

10 eletion and the hygromycin-resistant gene as selection marker.

11 ators of the rrnB operon, and a tetracycline selection marker.

12 ors has been hampered by the lack of patient selection marker.

13 frequencies >1%, thus obviating the need for selection markers.

14 tion and above-normal expression of positive selection markers.

15 a patients, flanked by positive and negative selection markers.

16 from low efficiency and a resultant need for selection markers.

17 loci and avoid untoward effects of retained selection markers.

18 and for multi-color tagging with orthogonal selection markers.

19 elements together with fluorescent and drug selection markers.

20 ansposon system for seamless removal of dual selection markers.

21 al center B cells and expression of positive selection markers.

22 ps, are few and largely depend on the use of selection markers(8-11).

23 c library we have constructed has a negative selection marker adjacent to the genomic insert, REC scr

24 We also discuss how different selection markers affect vector copy number in transform

25 hod is based on reconstitution of a dominant selection marker after Cre-mediated recombination of Lox

26 Retrofitted fosmid clones carry a selection marker and a phiC31 attB site, which facilitat

27 mproved protocol incorporating a Cas9-linked selection marker and a staggered transfection strategy p

28 ng the mouse genome using the HPRT gene as a selection marker and for transmission at a high frequenc

29 s; this involved using unc-119 as a positive-selection marker and GFP as a counter-selection marker w

30 usly designed for Cre, containing a positive selection marker and PhiC31 driven by a testis-specific

31 The selection marker and reporter (Luc-Ubi-Neo) in the BVDV

32 mfabI expands the limited repertoire of selection markers and allows for more efficient molecula

33 We designed a selection scheme with drug selection markers and Cre/loxP technology which allows s

34 the resulting engineered strain requires no selection markers and is unaffected by plasmid instabili

35 ay serve as a general platform with multiple selection markers and may be particularly useful for lar

36 of prokaryotic origin like vector sequences, selection marker, and reporter genes have been shown to

37 -agent regulations, the number of antibiotic selection markers approved for use in these bacteria is

38 to 20% without selection, and up to 60% when selection markers are used.

39 on efficiency when antibiotic based dominant selection markers are used.

40 Dual-conditional positive/negative selection markers are versatile genetic tools for manipu

41 when using Lymphocyte Activation Gene-3 as a selection marker, as few as 4,000 cells sufficed.

42 ry site (IRES) sequence followed by a Zeocin selection marker at the 3' end of the env sequence.

43 RNA sequencing (scRNA-seq) use a mix of size selection, marker-based white blood cell (WBC) depletion

44 that mfabI is not only an efficient plasmid selection marker, but it also possesses unique activity

45 rom hiPS cells using a triple combination of selection markers--CD34, neuropilin 1, and human kinase

46 Since this selection marker coexisted in trans with the helper viru

47 In contrast, benign selection markers complement GMOs with reduced fitness.

48 methods associated with the use of nutrient selection markers, complicated reporter analysis methods

49 luorescent protein (GFPuv) were constructed; selection markers comprised either mchI, conferring immu

50 Our approach utilizes a dual selection marker consisting of the puromycin resistance

51 plants, but very few of these combine plant selection markers, control of expression domains, access

52 We exploited Sox10 reporter and selection markers created by homologous recombination to

53 e incorporation of a red fluorescent protein selection marker enables combined utilization with widel

54 al transduction and that it can be used as a selection marker for functional heterogeneity of stem ce

55 fetal gonads and can be used as an effective selection marker for germ cell enrichment from different

56 We have also developed a new selection marker for transformation.

57 Selection markers for B. mallei are limited to Km and Ze

58 onally, there are few well developed counter-selection markers for use in Burkholderia.

59 ient, and extensible technology that enables selection marker-free, site-specific genome integration

60 es currently used, however, will introduce a selection marker gene at the modified site.

61 ous recombination followed by removal of the selection marker gene by Cre-loxP-mediated site-specific

62 T-overhang after digestion and the negative selection marker gene ccdB to eliminate the self-ligatio

63 rocess included the removal of a lox-flanked selection marker gene, bar.

64 les of phytochrome B (eYHB) as plant-derived selection marker genes in the model plant Arabidopsis (A

65 (and, at the same time, deletes the original selection/marker genes).

66 nti-EGFR antibodies in mCRC, their role as a selection marker has not been established in randomized

67 limited availability of heterologous counter-selection markers, here we explore novel DNA integration

68 suggests that IMH3 can be used as a dominant selection marker in C. albicans.

69 drial Alternative oxidase1a gene without HPT selection marker in rice enhanced tolerance to Hyg and a

70 the genetic background or the presence of a selection marker in the mutant mice could influence the

71 re no data available for the use of negative selection markers in monocotyledonous species.

72 ilised Flp recombinase to remove the unc-119 selection marker, in somatic cells, producing clean knoc

73 atistical methods for evaluating a treatment selection marker include assessing its prognostic value,

74 an CTLA4-Ig, and the dhfr gene as a positive selection marker is described.

75 Since no selection marker is used, recombinant clones are identif

76 S2 repeat, a TetO repeat, and inteins with a selection marker just downstream of the transcription st

77 Here we report the adoption of a novel selection marker, mfabI (mutant fabI) for plasmid propag

78 method depends on a thermostable endogenous selection marker operating at high temperatures combined

79 However, the existing assays use antibiotic selection markers or fluorescent proteins as reporters;

80 wo X-chromosomal counterselectable (negative selection) markers, PIGA and HPRT1, and additional cell

81 diated excision of the antibiotic-resistance selection marker present on the chromosomally integrated

82 diated excision of the antibiotic-resistance selection marker present on the chromosomally integrated

83 Here, we show how a widely used mammalian selection marker, puromycin N-acetyltransferase, can be

84 refinements in gene-transfer techniques, new selection markers, reliable reporter fusions and success

85 is cassette containing an inverted repeat of selection marker(s) is integrated into the genome.

86 Treatment selection markers, sometimes called predictive markers,

87 The single-gene dual selection marker tetA was translationally fused to green

88 d fly stocks and introduced a novel negative selection marker that drastically reduced the frequency

89 ecombinase target sites, a positive/negative selection marker that preserves the germline capacity of

90 r versions incorporate positive and negative selection markers that enable drug-based enrichment of r

91 Therefore Csf2rb can be used as a negative selection marker to enrich preleukemic progenitor cells

92 t BAC or PAC vectors do not have a mammalian selection marker, transfecting mammalian cells with gene

93 omology-directed repair with rates that make selection markers unnecessary.

94 i when a carbenicillin, but not a kanamycin, selection marker was used.

95 modular two color reporter system flanked by selection markers, we demonstrate that expression hetero

96 ke cells from lung digests using PDGFRB as a selection marker were expanded in culture as previously

97 Trophoblasts and B cells with separate selection markers were fused with polyethylene glycol.

98 sitive-selection marker and GFP as a counter-selection marker which is lost during homologous recombi

99 tep is monitored using positive and negative selection markers, which are the Kanamycin-resistance ge