ライフサイエンスコーパス: self-splicing

1 se a suicide inhibition mechanism to inhibit self-splicing.

2 ing group stabilization during both steps of self-splicing.

3 at catalyzes the two sequential reactions of self-splicing.

4 re NanGIR1 to group I introns that carry out self-splicing.

5 erved for protein-facilitated group I intron self-splicing.

6 ease reaction analogous to the first step of self-splicing.

7 onsecutive transesterification reactions for self-splicing.

8 group I intron undergoing the second step of self-splicing.

9 processes ranging from DNA synthesis to RNA self-splicing.

10 f the ligated exons after the second step of self-splicing.

11 at the 3'-splice site in the second step of self-splicing.

12 significantly more dynamic and complex than self-splicing.

13 -dependent splicing, relative to the rate of self-splicing.

14 e metal ion cluster, catalyzes both steps of self-splicing.

15 interferes with protein binding but not with self-splicing.

16 ween 4 and 50 mM Mg(2+), where the intron is self-splicing.

17 Exon mutations that inhibit self-splicing 100-fold in vitro were fully rescued when

18 During the initial event in protein self-splicing, a peptide bond to the nitrogen atom of an

19 the tRNA-Leu intron and tested the in vitro self-splicing ability of a diverse collection of these r

20 Thus, self-splicing activity is supported in the mammalian cel

21 HIV-1 TAR element was synthesized using the self-splicing activity of a group I permuted intron-exon

22 an evolutionary process in which the loss of self-splicing activity was compensated by the recruitmen

23 the bI4 group I intron and facilitating its self-splicing activity.

24 ange in the intron core that correlates with self-splicing activity.

25 f a ribozyme (D135) that is derived from the self-splicing ai5gamma intron from yeast mitochondria.

26 at excise themselves at the protein level by self-splicing, allowing the formation of functional, non

27 ively recognizes ligated exons formed during self-splicing and exhibits high fluorescent signal upon

28 To determine their role in self-splicing and in protein-assisted splicing, several

29 The mechanisms underlying the self-splicing and mobility of a few model group I intron

30 the first reports that certain RNAs mediate self-splicing and precursor tRNA processing reactions in

31 on with P10 after the first chemical step of self-splicing and release of the ligated exons after the

32 oup II introns, capable of carrying out both self-splicing and retromobility reactions, is hypothesiz

33 ly active structures that catalyze their own self-splicing and subsequent transposition into DNA.

34 s at the 5'-splice site in the first step of self-splicing, and P10 forms at the 3'-splice site in th

35 recover >60% enzymatic activity upon intein self-splicing at temperatures >59 degrees C.

36 mostable xylanase (XynB) with a thermostable self-splicing bacterial intein to control the xylanase a

37 scrimination of RNA from DNA templates using self-splicing bacterial introns, such that those nucleic

38 unrecognized role of the G.U wobble pair in self-splicing: breaking cooperativity in base pair forma

39 Most mutations at either position inhibit self-splicing, but can be suppressed by CYT-18.

40 est that a high Mg(2+) concentration induces self-splicing by globally stabilizing tertiary structure

41 ates its mutagenic effects by exploiting the self-splicing capabilities of split inteins.

42 des intron sequence, subclass, resident ORF, self-splicing capability, host gene, protein factor(s) i

43 ible reaction analogous to the first step of self-splicing: CCCUCUA (S) + [UC]G right harpoon over le

44 The psbA intron is the first example of a self-splicing chloroplast group II intron from any organ

45 In addition, even the best self-splicing constructs, which produced half as much mR

46 Here we evaluate the self-splicing efficiency of group I introns from transcr

47 by 1,000-fold, both resulting in compromised self-splicing efficiency.

48 protein cofactor of RNA catalysis harbors a self-splicing element called an intein.

49 by concomitant invasion of genes by group II self-splicing elements (which were to become introns in

50 an intron into foreign RNA or DNA by reverse self-splicing, followed by reverse transcription and rec

51 Group I introns are mobile, self-splicing genetic elements found principally in orga

52 There are four major classes of introns: self-splicing group I and group II introns, tRNA and/or

53 The Tetrahymena ribozyme derived from the self-splicing group I intron binds a 5'-splice site anal

54 rahymena L-21 ScaI ribozyme derived from the self-splicing group I intron catalyzes a reversible reac

55 Biochemical reactions catalyzed by a self-splicing group I intron derived from Pneumocystis c

56 A circularly permuted self-splicing group I intron from Anabaena was used to g

57 In the self-splicing group I intron from Tetrahymena thermophil

58 llel with the catalytic strategy used by the self-splicing group I intron from Tetrahymena.

59 inii is a mammalian pathogen that contains a self-splicing group I intron in its large subunit rRNA p

60 equence involved in rDNA-homing of a mobile, self-splicing Group I intron in Physarum was exploited.

61 Here we report the insertion of a self-splicing group I intron in the coding sequence of t

62 A potential RNA target in C. albicans is the self-splicing group I intron in the LSU rRNA precursor.

63 The P4-P6 domain RNA from the Tetrahymena self-splicing group I intron is an independent unit of t

64 The discovery of the RNA self-splicing group I intron provided the first demonstr

65 the CTD of an ancestral fungal mtTyrRS and a self-splicing group I intron was "fixed" by an intron RN

66 Self-splicing group I introns come in two flavours - tho

67 ribe the presence and characteristics of two self-splicing group I introns in the sole 23S rRNA gene

68 nas reinhardtii, the psbA gene contains four self-splicing group I introns whose rates of splicing in

69 Self-splicing group I introns, like other large catalyti

70 amino acids (Orf142) is interrupted by three self-splicing group I introns, providing the first examp

71 P. carinii, but not humans, contain RNA self-splicing group I introns, so these functionally imp

72 ucleotide bulge are conserved in many of the self-splicing group I introns.

73 n imaging study, we selected a member of the self-splicing group II intron family, which is hypothesi

74 tion pathway executed by the spliceosome and self-splicing group II intron ribozymes has prompted the

75 ver thirty years ago, after the discovery of self-splicing group II intron RNAs, the snRNAs were prop

76 ly, we have designed a derivative of a yeast self-splicing group II intron that is able to catalyze t

77 Self-splicing group II introns are found in bacteria and

78 Self-splicing group II introns have emerged as promising

79 erial endosymbiosis through fragmentation of self-splicing group II introns into a dynamic, protein-r

80 Self-splicing group II introns may be the evolutionary p

81 are adjacent folded hairpin substructures of self-splicing group II introns that appear to interact w

82 studied intensely is domain 5 (D5) from the self-splicing group II introns, which is at the heart of

83 he NMR and crystal structures of domain 5 of self-splicing group II introns.

84 We cloned the self-splicing group II Ll.LtrB intron from Lactococcus l

85 terminus of domain 6, during the folding of self-splicing group IIB intron constructs.

86 An in vitro form of the self-splicing group-I intron interrupting the Azoarcus t

87 In the first step of self-splicing, group I introns utilize an exogenous guan

88 ted for the recent, horizontal transfer of a self-splicing, homing group II intron from a cyanobacter

89 ns had surprisingly strong effects on Cr.LSU self-splicing; however, splicing of all but the P6 mutat

90 Self-splicing in optimal Mg2+ (>/= 150 mM) was tenfold s

91 The absence of introns that are not self-splicing in prokaryotes and several other lines of

92 adenosine base positions that compromise RNA self-splicing in the bI5 group I intron.

93 ied extensively under optimal conditions for self-splicing in vitro (42 degrees C and high magnesium

94 ron in vivo, although most mutations reduced self-splicing in vitro.

95 U) and ND1 introns, which are not detectably self-splicing in vitro.

96 long exon sequences are a major obstacle for self-splicing in vitro.

97 Both introns were found to be self-splicing in vivo and in vitro even though the termi

98 es a reaction analogous to the first step of self-splicing, in which a 5'-splice site analogue (S) an

99 The structures reveal dynamics during self-splicing, including large conformational changes of

100 gher Mg(2+) concentrations were required for self-splicing, indicating that compaction occurs before

101 They have also been identified in self-splicing inteins (protein introns).

102 Thus, this mobile, self-splicing intron also contains its own promoter for

103 h the spliceosome evolved from a prokaryotic self-splicing intron and an associated protein, it is a

104 ing and reverse cyclization reactions of the self-splicing intron from Tetrahymena thermophila.

105 BCV-1 has at least three types of introns, a self-splicing intron in a transcription factor-like gene

106 PBCV-1 has at least two types of introns--a self-splicing intron in a transcription factor-like gene

107 A self-splicing intron in the tRNA(fMet) gene of Synechocy

108 Insertion of a self-splicing intron into nickase blocks interference de

109 The Tetrahymena pre-rRNA self-splicing intron is shown to function in the unnatur

110 f substrate by the ribozyme derived from the self-splicing intron of Tetrahymena thermophila involves

111 the P4-P6 domain of the Tetrahymena group I self-splicing intron to yield the folding transition tim

112 The Tetrahymena thermophila group I self-splicing intron was the first ribozyme to be discov

113 There is a group I self-splicing intron within the gene encoding the D1 pro

114 mains 5 and 6 of the yeast ai5gamma group II self-splicing intron, revealing an unexpected two-nucleo

115 r interaction within the Tetrahymena group I self-splicing intron.

116 transpoviron, and the previously identified self-splicing introns and inteins constitute the complex

117 me (145-192 kilobases), with high density of self-splicing introns and inteins.

118 CPR organisms often have self-splicing introns and proteins encoded within their

119 Mechanistic comparisons between group II self-splicing introns and the spliceosome are therefore

120 Group II self-splicing introns are phylogenetically diverse retro

121 How homing endonucleases and self-splicing introns associate to form a composite self

122 omparative sequence analysis to show that in self-splicing introns belonging to subgroup IIB, the sit

123 Group II self-splicing introns catalyze autoexcision from precurs

124 Group I self-splicing introns catalyze their own excision from p

125 Our results show that both these non-self-splicing introns form most of the short range pairi

126 Interference analysis of the group I self-splicing introns from Tetrahymena and Azoarcus indi

127 potential for antisense targeting of group I self-splicing introns in fungal pathogens.

128 sted the presence of at least two additional self-splicing introns in this phage genome.

129 sive and ongoing horizontal gene transfer of self-splicing introns into extant fungal populations.

130 a genome editing method that embeds group 1 self-splicing introns into repetitive genetic elements t

131 Selfish elements in the form of self-splicing introns occur in many organellar genomes,

132 The active site of group I self-splicing introns occurs at the interface of two pro

133 rita namibiensis, regularly contain numerous self-splicing introns of variable length.

134 route to branched RNA that does not require self-splicing introns or spliceosomes would substantiall

135 Self-splicing introns populate several highly conserved

136 of retrotransposons are thought to be mobile self-splicing introns that actively propagate themselves

137 , revealing a striking diversity of reactive self-splicing introns with great promise as antifungal d

138 tured RNA elements that humans lack, such as self-splicing introns within key housekeeping genes in t

139 phates in a homologous structure of Group II self-splicing introns, long proposed to be the ribozyme

140 (ORFs) are frequently inserted into group I self-splicing introns.

141 teins that are encoded by genes with mobile, self-splicing introns.

142 e with homology to the maturases of group II self-splicing introns.

143 ns of genetic diversity in exons that border self-splicing introns.

144 introns, including spliceosomal and group II self-splicing introns.

145 hinery for both the spliceosome and group II self-splicing introns.

146 tential binding of drugs that target group I self-splicing introns.

147 ion, kinetic analysis reveals that AV intron self-splicing is activated only at elevated temperatures

148 However, we find that the extent of self-splicing is greatly influenced by sequences flankin

149 fied an allosteric group I ribozyme, wherein self-splicing is regulated by a distinct riboswitch clas

150 l tyrosyl-tRNA synthetase that also promotes self-splicing of group I intron RNAs by stabilizing the

151 Reverse self-splicing of group I introns has been demonstrated i

152 Self-splicing of introns post-transcription yields scarl

153 Self-splicing of Tetrahymena pre-rRNA proceeds in two co

154 We examined the role of omegaG in self-splicing of the 249-residue group I intron of the A

155 Self-splicing of the group I IVS from Tetrahymena thermo

156 ing experiments were performed and confirmed self-splicing of the introns.

157 re two distinct pathways for group II intron self-splicing: one involves 2'-OH attack and another inv

158 teins are mobile genetic elements capable of self-splicing post-translationally.

159 large family of phylogenetically widespread self-splicing protein catalysts that colonize diverse ho

160 Self-splicing protein elements (inteins) are attractive

161 Inteins are self-splicing protein elements found in viruses and all

162 Inteins, self-splicing protein elements, interrupt genes and prot

163 We redirected self-splicing protein inteins to create 'tadpoles', chim

164 Inteins (self-splicing 'protein introns') might offer a solution

165 the relationship between Hh proteins and the self-splicing proteins (i.e., proteins containing intein

166 The protein environment within self-splicing proteins appears to redirect the actions o

167 Inteins are phylogenetically diverse self-splicing proteins that are of great functional, evo

168 Self-splicing proteins, called inteins, are present in m

169 The percentage of transcripts that underwent self-splicing ranged from 0 to 50%, depending on the con

170 r for each splicing step and has a decreased self-splicing rate in vitro.

171 site for the first step of the RNA-catalyzed self-splicing reaction and thus is a model of a potentia

172 ate our understanding of the ribozyme to the self-splicing reaction and to further the mechanistic di

173 is also a suicide inhibitor of the intron's self-splicing reaction in vitro.

174 The transition state of the group I intron self-splicing reaction is stabilized by three metal ions

175 we are able to extend the mechanism for the self-splicing reaction of this intron by proposing two d

176 As part of its self-splicing reaction, this ribozyme catalyzes phosphor

177 s and raises new questions about the overall self-splicing reaction.

178 tore host protein function through a protein self-splicing reaction.

179 been used to dissect aspects of the group I self-splicing reaction.

180 helix that position the P1 substrate for the self-splicing reaction.

181 Self-splicing reactions also accompany the maturation of

182 n contrast to results reported for analogous self-splicing reactions using a Tetrahymena ribozyme.

183 I intron promotes catalysis and coordinates self-splicing reactions, we determine the structures of

184 chanism are conserved between Hh-C17 and the self-splicing regions of inteins, permitting reconstruct

185 e the 5'-exon-intron recognition duplex of a self-splicing ribozyme as a model system to study the in

186 The Tetrahymena ribozyme is a model group I self-splicing ribozyme that has been shown to be useful

187 Group II introns are self-splicing ribozymes and mobile genetic elements.

188 Group II introns are ubiquitous self-splicing ribozymes and retrotransposable elements e

189 Our findings indicate that some self-splicing ribozymes are not selfish elements but are

190 Group I self-splicing ribozymes commonly function as components

191 Group II introns are self-splicing ribozymes that catalyze their own excision

192 Group II introns are self-splicing ribozymes that share a reaction mechanism

193 intron ribozymes and a catalytically active self-splicing RNA containing full-length intron and shor

194 Folding of the Tetrahymena self-splicing RNA into its active conformation involves

195 The Tetrahymena thermophila self-splicing RNA is trapped in an inactive conformation

196 Group II introns are self-splicing RNA molecules that also behave as mobile g

197 Group II introns are self-splicing RNA molecules that are of considerable int

198 We show that the Tetrahymena self-splicing RNA partitions into a population that rapi

199 zoarcus tRNA(Ile) is an exceptionally stable self-splicing RNA.

200 ng 43B site; and (c) the group I intron is a self-splicing RNA.

201 Group II introns are self-splicing RNAs found in eubacteria, archaea, and euk

202 Small molecule targeting of self-splicing RNAs like group I and II introns has been

203 Self-splicing RNAs must evolve to function in their spec

204 Group II introns are self-splicing RNAs that are commonly found in the genes

205 Since the discovery of self-splicing RNAs, it has been suspected that the snRNA

206 yme catalyzing 5' maturation of tRNAs) and a self-splicing rRNA of Tetrahymena, respectively.

207 Use of the naturally split, self-splicing Synechocystis sp. PCC6803 DnaE intein perm

208 The self-splicing system that we describe should facilitate

209 2.8 A crystal structure of one domain of the self-splicing Tetrahymena group I intron was reported.

210 reporter ribozyme constructs consist of the self-splicing Tetrahymena thermophila group I intron rib

211 of these oligonucleotides inhibit precursor self-splicing via a suicide inhibition mechanism.

212 e tested for their ability to inhibit intron self-splicing via a suicide inhibition mechanism.

213 e binding steps of Tetrahymenagroup I intron self-splicing, which have positive or zero DeltaH terms,

214 strongly in CBP2-dependent splicing than in self-splicing, yield a cost for protein facilitation of