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1 direct DNA sequencing using a capillary DNA sequencer.
2 gle run of the 454 Life Sciences (Roche) FLX sequencer.
3 of the isolated DNA by a massively parallel sequencer.
4 segregating line are analyzed on a capillary sequencer.
5 d polymerase chain reaction and an automated sequencer.
6 patible with any four-color fluorescence DNA sequencer.
7 onal sample capacity on a commercial protein sequencer.
8 on on an ABI 310 Genetic Analyzer or ABI 377 Sequencer.
9 l format liquid handler and an automated DNA sequencer.
10 mine their elution profile on an ABI protein sequencer.
11 s is accomplished with a dual dye Li-cor DNA sequencer.
12 tion fragments using an automated Li-cor DNA sequencer.
13 acid, blocked with OPA, and reapplied to the sequencer.
14 are determined using an ABI377 automated DNA sequencer.
15 repeat unit differences on an automated DNA sequencer.
16 t further purification to an automated Edman sequencer.
17 analyzed and quantified on an automated DNA sequencer.
18 as sequenced using an automated fluorescence sequencer.
19 low-cost Oxford Nanopore Technologies MinION sequencer.
20 quired using a four-color slab gel automated sequencer.
21 nd libraries which were sequenced on a MiSeq sequencer.
22 ly phase 2-200 kb targets using a short-read sequencer.
23 ing targeted enrichment on an Illumina MiSeq sequencer.
24 -by-step manually or using a next-generation sequencer.
25 d equipment, as well as access to a Nanopore sequencer.
26 ng with long-read sequencing with a portable sequencer.
27 using the Oxford Nanopore Technologies' Mk1B sequencer.
28 million cDNA reads per run on the Sequel IIe sequencer.
29 ng on an Oxford Nanopore Technologies MinION sequencer.
30 sing the Oxford Nanopore Technologies MinION sequencer.
31 for the Oxford Nanopore Technologies MinION sequencer.
32 anscripts and sequenced on a high-throughput sequencer.
33 epending on the number of passes through the sequencer.
34 rial 16S rRNA genes were analyzed on a MiSeq sequencer.
35 t by laboratories with access to a capillary sequencer.
36 velet transform of read information from the sequencer.
37 sequencing; "DIP-SC-seq") on the Ion Proton sequencer.
38 ects and sequenced them on an Illumina MiSeq sequencer.
39 ere pooled and sequenced with the 454 GS FLX sequencer.
40 cost reductions afforded by next generation sequencers.
41 rt-read data sets, all generated by Illumina sequencers.
42 metagenomic datasets from different type of sequencers.
43 o third- and fourth-generation automated DNA sequencers.
44 quality values that come along with Beckman sequencers.
45 with different sizes can be resolved by DNA sequencers.
46 d elements presents special problems for DNA sequencers.
47 n is compatible with most Applied Biosystems sequencers.
48 rovement in the performance of automated DNA sequencers.
49 le-stranded M13mp18 template and ABI 373 DNA sequencers.
50 med using both Illumina HiSeq X10 and MinION sequencers.
51 bilities that threaten the security of these sequencers.
52 ure of DNA sequencing errors incurred on DNA sequencers.
53 d by Pacific Biosciences and Oxford Nanopore sequencers.
54 ling compared to traditional high-throughput sequencers.
55 o physical limits on molecular throughput of sequencers.
56 RNAs and RNA Sequins with nanopore long-read sequencers.
57 ay be implemented in practice using nanopore sequencers.
58 next generation sequencing and/or capillary sequencers.
59 ring the volume of data produced by Illumina sequencers.
60 use of the multiplexing functionality of the sequencers.
61 mples at low cost on desktop next-generation sequencers.
63 uated the method by using the Illumina MiSeq sequencer (40 smear-positive respiratory samples obtaine
64 series of barcoded sequencing using the GS20 Sequencer (454/Roche), we found that over 99.8% of obtai
65 an be used to assess, calibrate, and monitor sequencer accuracy, and to computationally suppress sequ
66 ique feature of Oxford Nanopore Technologies sequencers, adaptive sampling, allows precise DNA molecu
67 ital abnormalities using the MinION nanopore sequencer and a novel computational pipeline-NanoSV.
68 or liquid storage, and read using a nanopore sequencer and a novel, minimal preparation protocol.
69 the method using the ABI 373A automated DNA sequencer and accompanying Genescan/Genotyper software r
70 fragments separated on an ABI 377 automated sequencer and analyzed with Genescan version 2.1 softwar
71 of patient genome sequencing with a nanopore sequencer and demonstrate the value of long-read sequenc
73 laboratory with access to a high-throughput sequencer and high-power computing can adapt this protoc
76 ragment size are generated by an ABI 377 DNA sequencer and the GeneScan analysis software and then pr
77 croscope head was placed in an automated DNA sequencer and translated across a 21-cm-wide gel plate i
78 tive, base-by-base error predictors for this sequencer and used a variant of the phred binning algori
79 rate to be ~ 10 per million (pm) and 1.4% of sequencers and 2.7% of flow cells have error rates > 100
80 the review presents a look at available DNA sequencers and array technology and concludes with a loo
82 al genomes have been sequenced by short read sequencers and have resulted in a mix of contigs that de
83 to molecule length across different Illumina sequencers and illustrate the impacts on interpretation
84 s of the reads produced by second generation sequencers and is essential for de novo assembly of geno
85 characterized in commercially available DNA sequencers and showed uniform electrophoretic mobilities
87 ranes for chemical separations and molecular sequencers and to even mimic neuromorphic computing elem
89 he read (base quality scores reported by the sequencer) and the alignment (number of matches, mismatc
91 ons can be loaded directly onto an automated sequencer, and the number of alleles, allele size range,
92 fferent types of data produced by second-gen sequencers, and the latest assembly algorithms designed
93 , Santa Monica, Calif.) with the ABI 377 DNA sequencer (Applied Biosystems Inc.), the HIV PRT GeneChi
94 g ladders were analyzed using an ABI 373 DNA sequencer (Applied Biosystems, Foster City, CA, USA).
95 ach, the optics built into a high-throughput sequencer are used to visualize in vitro binding of a pr
100 uence data files produced by MegaBace or ABI sequencers as well as Staden SCF trace files and plain t
101 plification and loading onto next-generation sequencers, as well as computational normalization, can
102 Glycomic analysis was performed using DNA sequencer associated fluorophore associated capillary el
103 Glycomic analysis was performed using DNA sequencer-associated fluorophore-associated capillary el
107 that capture the error modes of the nanopore sequencer before running it through a sequence aligner.
108 f colony sequencing with the capillary array sequencer, both the front end and the back end of DNA se
109 sequence short DNA molecules on a long-read sequencer by randomly ligating them to form long molecul
113 The massive capacity of next-generation sequencers can be harnessed for sequencing specific geno
116 lays MinKNOW (the software that controls ONT sequencers) classifications on the signal trace and can
117 N-terminally blocked can be removed from the sequencer, cleaved with acetic acid, blocked with OPA, a
118 ing sequencing resources and availability of sequencers critical factors for conducting deep transcri
120 es the effective analysis of targeted clonal sequencer data without dedicated computational infrastru
121 d clean sequencing data on a fluorescent DNA sequencer, eliminating the false terminations and backgr
124 The Oxford Nanopore Technologies MinION sequencer enables the selection of specific DNA molecule
125 tosampler attaches to a standard ABI Procise sequencer, enabling a single-sample cartridge to hold up
126 ipment beyond a low-overhead Oxford Nanopore sequencer, enabling most labs to flexibly process hundre
127 oyed nanopore adaptive sampling (NAS), an on-sequencer enrichment method that selects for target DNA
128 ch institutions in 18 countries revealed the sequencer error rate to be ~ 10 per million (pm) and 1.4
130 ms and insertions/deletions (indels), and by sequencer errors make alignment a difficult and computat
131 alm of education, portable tools such as DNA sequencers facilitate in situ hands-on training in real-
132 is made compatible with the Roche/454 Genome Sequencer FLX Titanium next-generation sequencing techno
134 ectability technology enabled through MinION sequencer for a natural low biomass setting, we characte
136 Nanopore Technologies (ONT) MinION long-read sequencer for routine WGS by sequencing the reference sa
138 d a commercial zero-mode waveguide-based DNA sequencer for use as a versatile instrument for single-m
145 h was adapted to the Hewlett-Packard G 1009A sequencer, has been shown to identify two or three cycle
147 N50 >= 100 kb) produced from Oxford Nanopore sequencers have improved genome assemblies in recent yea
148 sequencing modification sites on commercial sequencers have not been developed beyond the epigenetic
151 sequences using a capillary electrophoresis sequencer in a manner that allows high-throughput nucleo
152 evaluated the performance of the MinION DNA sequencer in-flight on the International Space Station (
153 technologies exist, the ubiquity of Illumina sequencers in sequencing core facilities and the high ca
154 hod of transcriptome sequencing for Illumina sequencers in which the reverse transcription reaction i
155 our experience of using the MinION, a mobile sequencer, in a 13-week academic course for undergraduat
156 features for the industrial scale PromethION sequencer, including standard and "barcode-aware" adapti
157 Oxford Nanopore Technologies (ONT) portable sequencer is a promising platform for cost-effective app
158 -read single-molecule Oxford Nanopore MinION sequencer is able to identify and quantify complex isofo
159 triction endonucleases, and an automated DNA sequencer is employed to determine the size of the label
160 accuracy of base calls produced by Illumina sequencers is adversely affected by several processes, w
161 uences in these regions with next generation sequencers is challenging, and requires a different set
162 Oxford MinION, the first commercial nanopore sequencer, is also the first to implement molecule-by-mo
163 use the ONT MinION, an error-prone long-read sequencer, is associated with little to no capital cost.
164 rnative, Oxford Nanopore Technologies MinION sequencer, is quickly gaining popularity because of the
165 s, etc), instrumentation (mass spectrometer, sequencer), keywords and other provided annotations.
166 ection method using multicapillary automated sequencers, known as conformation-sensitive capillary el
167 able on either benchtop or portable nanopore sequencers, making this method directly applicable for d
169 acy, coupled with higher throughput nanopore sequencers, mean that human genome sequencing at scale i
170 by two PCR-based methods, the "long distance sequencer" method and the "promoter finder" method.
171 with the low-cost, portable next generation sequencer MinION from Oxford Nanopore Technologies had s
172 rmatics workflows using a long-read nanopore sequencer (MinION) for Y. pestis (6.5 h) and B. anthraci
173 designed to distinguish true mutations from sequencer misreads and PCR misincorporations, we achieve
174 wed by sequencing on a 454 Life Sciences FLX sequencer, most sequence reads represented selection tar
176 g was performed using an Illumina NextSeq500 sequencer on the strain both with and without exposure t
177 g was performed using an Illumina NextSeq500 sequencer on the strain with and without exposure to mer
178 procedures for preparing DNA for running on sequencers or subsequent analysis; it also includes info
180 preparation methods for the portable MinION sequencer (Oxford Nanopore Technologies) and the Illumin
181 S. cerevisiae DNA samples showed that MinION sequencer (Oxford Nanopore Technologies) can unequivocal
182 ts and high sequencing error rates of modern sequencers present new computational challenges in data
183 followed by fragment size analysis on a DNA sequencer produces profiles for targeted genes, which ca
184 ia and the Applied Biosystems 373A automatic sequencers, producing data that is comparable with cycle
185 ntified by analysis of the Edman degradation sequencer product because the palmitoylated sequencer pr
186 sequencer product because the palmitoylated sequencer products were lost during the final derivatiza
187 e the front-end tasks to capillary-array DNA sequencers, protocols for directly sequencing the plasmi
188 ase color reads produced by lifetech's SOLiD sequencer provide unreliable results when translated to
190 G6 and three other ET primers on a capillary sequencer provided DNA sequences with 99% accuracy in th
191 The performance of this miniaturized DNA sequencer provides a benchmark for predicting the ultima
192 cal run with these ET primers on a capillary sequencer provides DNA sequences with 99% accuracy in th
196 prototype microfluidic system for preparing sequencer-ready DNA libraries for analysis by Illumina s
197 lecular reaction, Reflex, to derive shorter, sequencer-ready, daughter polymerase chain reaction prod
198 tem; it can align the reads from a human WGS sequencer run-over 500 million 150nt paired-end reads-in
199 tained from gel filtration necessitated dual sequencer runs of radioactive peptides, one for sequence
200 by gel filtration on Sephadex LH60 and dual sequencer runs, positioned the 3H-labeled palmitoylated
203 labeled PCR primers and the Perkin-Elmer DNA sequencer so that unknown-specimen fingerprints are iden
205 thms and the recent development of long-read sequencers, split mapping will soon be the preferred met
209 enomic sequencing based on new generation of sequencers, such as the 454-sequencing system provides a
210 MinION is a USB-connected, portable nanopore sequencer that permits real-time analysis of streaming e
211 s principle has been implemented in portable sequencers that use enzymes to move DNA or RNA through h
213 ify the millions of reads output from modern sequencers, the combination of incomplete databases, sim
214 assays take advantage of Illumina short-read sequencers, the predominant short-read sequencing techno
215 ed down to 1-bp resolution with a commercial sequencer, thereby reconciling haplotype-phased chromoso
218 ore Technologies (ONT) is the first nanopore sequencer to be commercialized and is now available to e
219 ploy a platform based on a modified benchtop sequencer to conduct a massively parallel aptamer screen
221 2019) repurpose a common next-generation DNA sequencer to enable high-throughput protein biochemical
222 onucleotide primers of a next-generation DNA sequencer to function as both a capture and sequencing s
223 rimers and amplicon sizing on a Sanger-style sequencer to generate fluorescent PCR ribotyping data.
224 high-throughput short-read second-generation sequencer to generate over 100 kb of long-range sequenci
225 Here, we use the Oxford Nanopore MinION sequencer to identify 7,899 'full-length' isoforms expre
226 ucts of the reactions were analysed on a DNA sequencer to identify the presence of two or three copie
227 te-converted fragment library with the SOLiD sequencer to investigate genome-wide methylation levels.
228 e genome human FASTQ from Illumina's NovaSeq sequencer to less than 7 GB, around 1.6x smaller than pr
229 RAP) assay by adapting a high-throughput DNA sequencer to quantify the binding of fluorescently label
231 , we use the MinION single-molecule nanopore sequencer to uncover CN heterogeneity in clonal populati
233 n these hackathons, the students used MinION sequencers to generate and analyze their own data and ga
234 rom paired samples and sequenced on Illumina sequencers to high coverage depths of ~ 930X (tumor) and
236 ed a manifold learning algorithm called "the Sequencer" to simultaneously analyze thousands of seismo
237 matically, by using an ALF Express automatic sequencer, to confirm the mycoplasma species and to iden
239 s with a PE Applied Biosystems automated DNA sequencer, two independent incision events, one in each
240 ic value cannot be obtained from acquiring a sequencer unless it is accompanied by an equal investmen
243 2000s greatly accelerated development of DNA sequencers, ushering in the era of "Next Generation Sequ
244 mized the performance of the MinION nanopore sequencer using M13 genomic DNA and used expectation max
245 obust analysis tools for next-generation DNA sequencers using the functional programming philosophy o
247 subsequently been implemented on fluorescent sequencers we felt that there was a need to develop and
250 sequencing a common DNA library on different sequencers, we demonstrate that sequencers with high err
251 read length of 485 bp, and ABI3700 capillary sequencers, we have generated 449,234 nonredundant mouse
252 As commercially available high-throughput sequencers were first released less than 15 years ago, w
254 wn as phasing, is achievable on a short-read sequencer when using a library preparation method that c
255 rors that occur in the sequencing instrument/sequencer, which is critical for next-generation sequenc
256 torage system that uses error-prone nanopore sequencers, while still producing error-free readouts wi
257 vided DNA sequences on a four-color capilary sequencer with 100% accuracy in the first 500 bases.
258 esolution in a capillary electrophoresis DNA sequencer with laser-induced fluorescence detection.
259 on different sequencers, we demonstrate that sequencers with high error rates have reduced overall se
260 Using Illumina Novaseq X Plus and ONT P2 sequencers with R10.4.1 chemistry, we set a new benchmar
261 (LIMS) system for Oxford Nanopore Technology sequencers, with real-time metrics and analysis availabl