ライフサイエンスコーパス: serotyping

1 l composition forms the basis for Salmonella serotyping.

2 ) and pertussis toxin (ptxA) genotyping, and serotyping.

3 ational Reference Center for Pneumococci for serotyping.

4 isolates tested in comparison to traditional serotyping.

5 reatly reduce our reliance upon conventional serotyping.

6 ts in complete concordance with conventional serotyping.

7 rphism in cps genes and correlates well with serotyping.

8 on reference laboratory for confirmation and serotyping.

9 ting ability of PFGE was better than that of serotyping.

10 ), rapid amplified polymorphic DNA, and Lior serotyping.

11 e a simple and stable surrogate for capsular serotyping.

12 tate epidemiological studies of pneumococcal serotyping.

13 pulsed-field gel electrophoresis (PFGE) and serotyping.

14 There are several methods available for serotyping.

15 ns were also characterized by auxotyping and serotyping.

16 ys and whole-genome sequencing were used for serotyping.

17 nd 99% (99% to 100%) specificity compared to serotyping.

18 mical tests and lipopolysaccharide O-antigen serotyping.

19 cificity versus results of standard Quellung serotyping.

20 ods were used for pneumococcus isolation and serotyping.

21 serovars of H. parasuis, for rapid molecular serotyping.

22 ere processed for pneumococcal isolation and serotyping.

23 sed VT detection by 31.5% over that by latex serotyping.

24 FGE), multilocus sequence typing (MLST), and serotyping.

25 E, 99.7%; MEE, 99.4%; ribotyping, 98.8%; MAb serotyping, 75.8%; MAb serotyping and/or serosubtyping 9

26 resis, pulsed-field gel electrophoresis, and serotyping, 84% (52/62) of the isolates available for st

27 Although molecular serotyping added 7% (737/11,224) serotyping data, the in

28 lationship could not have been determined by serotyping alone (P = 0.320), demonstrating the value of

29 om amplified polymeric DNA (RAPD) and Penner serotyping analyses.

30 2016, 13 468 (94.9%) were characterized with serotyping and 12 235 (86.2%) with antibiotic susceptibi

31 uality control (QC) program for pneumococcal serotyping and antibiotic susceptibility testing was inc

32 Serotyping and antibiotic susceptibility were performed

33 demonstrate a high degree of correlation of serotyping and antimicrobial susceptibility testing resu

34 As part of ongoing national surveillance, serotyping and antimicrobial susceptibility testing were

35 Pneumococcal serotyping and antimicrobial susceptibility testing were

36 Phenotypic serotyping and antimicrobial susceptibility testing were

37 monocytogenes isolates were characterized by serotyping and automated EcoRI ribotyping.

38 To improve sensitivity and specificity of serotyping and for early detection, a multiplex PCR assa

39 e data from PCR serotyping with conventional serotyping and found that PCR serotyping was nearly as d

40 t the first distribution using their current serotyping and genotyping methods.

41 lar techniques were more discriminatory than serotyping and identified differences between some isola

42 L. monocytogenes is usually characterized by serotyping and is subtyped by using pulsed-field gel ele

43 emonstrate concordance between real-time PCR serotyping and latex agglutination.

44 North America (mainly the United States) by serotyping and MLST and to investigate associations betw

45 Our study demonstrates the use of serotyping and MLST to differentiate pathogenic from com

46 break-associated cases were identified using serotyping and molecular subtyping procedures.

47 Serotyping and molecular typing by pulsed-field gel elec

48 Meningococcal isolates were identified by serotyping and multilocus enzyme electrophoresis.

49 Serotyping and multilocus sequence typing (MLST) are pri

50 Serotyping and other phenotypic methods are often used t

51 vestigations and could complement or replace serotyping and other subtyping methods.

52 %) had concordant results between phenotypic serotyping and PCR.

53 While serotyping and phage typing have been used widely to cha

54 ted a genetic linkage analysis that included serotyping and phenotyping 421 individuals from 39 famil

55 water-borne or food-borne diarrhea, such as serotyping and pulsed-field electrophoresis.

56 y, and the results were compared to those of serotyping and pulsed-field gel electrophoresis (PFGE).

57 Serotyping and pulsed-field gel electrophoresis were per

58 inical isolates of Listeria monocytogenes by serotyping and restriction fragment length polymorphism

59 Serotyping and serogrouping of Salmonella isolates was p

60 ater discriminatory abilities than MAb-based serotyping and serosubtyping or ITS PCR-RFLP.

61 ive methods: monoclonal antibody (MAb)-based serotyping and serosubtyping, DNA macrorestriction analy

62 ied polymorphic DNA assay (two primers), and serotyping and serosubtyping.

63 and serosubtype determination) by MAb-based serotyping and serosubtyping.

64 Serotyping and susceptibility testing of isolates were p

65 Isolates underwent serotyping and susceptibility testing.

66 al and pleural fluid) and were available for serotyping and susceptibility testing.

67 ns the extensive cross-reactivity based upon serotyping and the lack of consistent association of giv

68 DNA microarray was used for molecular serotyping and to infer genetic lineage (Global Pneumoco

69 nd ST74 genomes, highlighting challenges for serotyping and vaccine development, and has implications

70 demonstrated cyclomodulin cytotoxicity, and serotyping and whole genome sequencing were consistent w

71 asopharyngeal isolates were characterized by serotyping and whole-genome sequencing.

72 typing of Salmonella is better than that of serotyping and/or PFGE typing.

73 ibotyping, 98.8%; MAb serotyping, 75.8%; MAb serotyping and/or serosubtyping 97.5%; and ITS PCR-RFLP,

74 loci distinguished by serological reactions (serotyping) and by differences in the amino acid sequenc

75 strains undistinguishable by PFGE typing and serotyping), and it may be of value during investigation

76 entiated several strains undifferentiable by serotyping, and 78 distinct PFGE types were identified a

77 Pulsed-field gel electrophoresis (PFGE), serotyping, and antibiotic susceptibility testing were p

78 Automated blood culture, manual speciation, serotyping, and antimicrobial susceptibility testing wer

79 by pulsed-field gel electrophoresis (PFGE), serotyping, and antimicrobial susceptibility testing.

80 ut different from the results of traditional serotyping, and five isolates (1.3%) generated false-neg

81 ence genotyping, hemagglutination testing, O serotyping, and PCR-based phylotyping.

82 pharmacogenomic (PGx) markers, blood antigen serotyping, and polygenic risk scores in 100 individuals

83 ased surveillance associated with gonococcal serotyping, and provides useful information about the mo

84 ility, pneumococcal surface protein A (PspA) serotyping, and pulsed-field gel electrophoresis (PFGE)

85 d by culture, qPCR, whole genome sequencing, serotyping, and reverse transcription qPCR.

86 in reaction (qPCR), whole genome sequencing, serotyping, and reverse-transcription qPCR.

87 es were indistinguishable by PFGE, somatic O serotyping, and sequencing of the 582-bp region of the f

88 The rapid isolation, serotyping, and vaccine matching of FMD virus from disea

89 rol and Prevention for species confirmation, serotyping, and whole genome sequencing of select isolat

90 oung asymptomatic carriers in Chile, we used serotyping, antibiotic susceptibility testing, and genot

91 ary 2003 and June 2004 were characterized by serotyping, antibiotic susceptibility testing, and pulse

92 ins PIA and PIB of Neisseria gonorrhoeae are serotyping antigens for the serovar classification syste

93 Serotyping, antimicrobial susceptibility testing, and wh

94 The isolates were characterized by serotyping, antimicrobial-susceptibility testing, phage

95 commercial Salmonella somatic and flagellar serotyping antisera to in-house-prepared antisera from t

96 To develop a PCR- and sequence-based serotyping approach, we integrated available data source

97 As the development of molecular serotyping approaches is critical for Salmonella spp., w

98 22/50 [16 to 44%]), and the three different serotyping approaches that used broth enrichment increas

99 However, existing methods for serotyping are limited in their ability to identify co-c

100 f identification, including biochemistry and serotyping as well as whole-genome sequencing analyses,

101 The multiplex serotyping assay consists of 24 assays specific for 36 s

102 The specificity of the serotyping assay is fully characterized with pneumococci

103 ) is an automated, monoclonal antibody-based serotyping assay that uses culture lysates of Streptococ

104 es the need to validate any new pneumococcal serotyping assay with a large number of clinical isolate

105 lysate preparation protocol and a multiplex serotyping assay.

106 ype compared to their corresponding monoplex serotyping assay.

107 Triplex serotyping assays also showed 100% agreement for each se

108 Advantages over other serotyping assays are described.

109 ranged from 11 to 130 CFU/mL for the triplex serotyping assays.

110 test were sent for confirmatory testing and serotyping at the Hinton State Laboratory Institute (HSL

111 Compared to serotyping, both tests had 96% sensitivity; specificity

112 as pulsed-field gel electrophoresis and O:H serotyping but more convenient.

113 t a level comparable to that of conventional serotyping, but at a fraction of both the cost and time

114 ate, could be used as a simple surrogate for serotyping by clinical microbiology laboratories that ar

115 Molecular serotyping by microarray was used to determine pneumococ

116 For molecular serotyping by microarray, we used the BUGS Bioscience Se

117 nwatch vs MinION-ONT sequencing coupled with serotyping by Pathogenwatch from pneumococcal assembled

118 re were presumed to be pneumococci underwent serotyping, capsular staining, pulsed-field gel electrop

119 This system should be very useful in serotyping clinical isolates for evaluating pneumococcal

120 his TAC method yields fast and comprehensive serotyping compared to the standard method and may be us

121 Repeated serotyping confirmed only 69.0% of isolates.

122 re subjected to bacteriological examination, serotyping, congo-red binding assay, antibiogram-testing

123 Our results show that comparable serotyping data can be obtained in different laboratorie

124 Serotyping data showed an incidence rate of 51.2% for no

125 nes isolates agreed with slide agglutination serotyping data, and 100 previously uncharacterized isol

126 h molecular serotyping added 7% (737/11,224) serotyping data, the inability to resolve 40% of samples

127 veillance and disease control in addition to serotyping data.

128 Serotyping dengue virus (DENV) from suspect human specim

129 ted for the C-prM protocol for detecting and serotyping dengue viruses.

130 rmed using Whole-Genome Sequencing (WGS) for serotyping, determination of virulence factors, antimicr

131 cribes SerotypeFinder, an essential guide to serotyping E. coli in the 21st century.

132 Rep-PCR typing uncovered several historical serotyping errors and provided presumptive serotype assi

133 al pathogenic E. coli (ExPEC) and superseded serotyping for certain isolates with ambiguous or non-H7

134 he inhibition ELISA method will be useful in serotyping for epidemiological studies, assessing virule

135 gical grouping, T-agglutination pattern, and serotyping for M protein or opacity factor.

136 Serotyping for myositis-specific/myositis-associated aut

137 Serotyping forms the basis of national and international

138 al swabs for pneumococcal identification and serotyping from residents of all ages at 8 rural village

139 In silico serotyping from WGS has not yet been applied to long-rea

140 However, serotyping generally requires either latex agglutination

141 a general design strategy for detecting and serotyping genetically diverse viruses.

142 This molecular serotyping/genotyping scheme is well suited to rapid cha

143 A pneumococcal serotyping/genotyping system (PSGS) was developed based

144 Confirmatory identification and serotyping/grouping of Streptococcus pneumoniae, N. meni

145 Where possible, serotyping/grouping was performed to determine the speci

146 r polymerase chain reaction; where possible, serotyping/grouping was performed.

147 ecies-specific polymerase chain reaction and serotyping/grouping were conducted to determine specific

148 Serotyping/grouping, antimicrobial susceptibility testin

149 ion could be a valid and reliable method for serotyping H. influenzae if the test was performed corre

150 Although serotyping has been a useful tool, this method can be in

151 Serotyping has been of great value in understanding the

152 Serotyping has been used in the past to assist epidemiol

153 ccessible, and more recently developments in serotyping have focused on molecular techniques.

154 ce analyses of rep-PCR and AFLP with somatic serotyping indicate that, in general, somatic serotyping

155 Serotyping indicated that 12 of 15 cases tested were cau

156 erotyping indicate that, in general, somatic serotyping is a poor indicator of genetic relatedness am

157 Serotyping is a universally accepted subtyping method fo

158 Latex serotyping is accurate in identifying vaccine serotypes

159 rol of the hundreds of antisera required for serotyping is difficult and time-consuming.

160 Accurate serotyping is essential to monitor the changes in the se

161 it is useful for a laboratory where standard serotyping is not available.

162 Antibody-based serotyping is unworkable when specimens are urine or vag

163 types of data for DQA1 and DRB1 showed that serotyping led to generally lower estimates of S.

164 (DBCT) identification method with Lancefield serotyping (LS).

165 Here, we employed a novel serotyping method and NMR spectroscopy to examine clinic

166 have recently developed a rapid pneumococcal serotyping method called "multibead assay" based on a mu

167 , a rapid and simple LAMP-based pneumococcal serotyping method has been developed.

168 To develop a scalable and portable serotyping method that overcomes these challenges, we em

169 it the role of this gene in a sequence-based serotyping method.

170 resents an attractive alternative to current serotyping methods and may allow for improved acquisitio

171 Traditional serotyping methods are cumbersome and insufficient for d

172 Conventional serotyping methods are laborious and expensive.

173 era and highlight the idea that conventional serotyping methods are not capable of recognizing all pu

174 Conventional serotyping methods assume that each serotype is a geneti

175 Because classic pneumococcal serotyping methods cannot distinguish between serotypes

176 the identification of the best pneumococcal serotyping methods for carriage studies.

177 hat can help with the selection of molecular serotyping methods to be used by different laboratories.

178 Conventional serotyping methods using rabbit polyclonal or mouse mono

179 To conclude, all three serotyping methods were highly concordant in identifying

180 is that not all of the available alternative serotyping methods were included.

181 Twenty pneumococcal serotyping methods were used to test 81 laboratory-prepa

182 For the alternate serotyping methods, the overall sensitivity ranged from

183 isolates tested in parallel with traditional serotyping methods.

184 e variants indistinguishable by conventional serotyping methods.

185 Isolates were characterised by serotyping, multilocus sequence typing, and antimicrobia

186 Using serotyping, multilocus sequence typing, and whole-genome

187 Serotyping, multiple high-resolution molecular genotypin

188 sequence data whilst preserving the best of serotyping nomenclature.

189 ethods and the reference method (traditional serotyping of >100 colonies from each sample).

190 HLA class I serotyping of 158 chronically infected patients revealed

191 Serotyping of 178 patient isolates revealed that 34% had

192 ELISA serotyping of 89 of 101 L. monocytogenes isolates agreed

193 Serotyping of Actinobacillus pleuropneumoniae is based o

194 mponent of the thermostable antigen used for serotyping of C. jejuni.

195 A method is described for the serotyping of Cryptococcus neoformans based on direct an

196 Serotyping of DV is accomplished by a judicious design o

197 lent mismatch discrimination ability permits serotyping of DVs in one tube with excellent sensitivity

198 n ultrasensitive assay for the detection and serotyping of DVs is described in the report.

199 Serotyping of GBS isolates is important for understandin

200 Serotyping of GBS isolates was done in South Africa.

201 for the rapid detection, identification, and serotyping of human adenoviruses.

202 clinical laboratory isolation, and continued serotyping of isolates in public health laboratories.

203 We confirmed serotyping of isolates with sequencing of wcjE alleles.

204 ed incidence rates and adjusted rate ratios, serotyping of isolates, and comparison of secular trends

205 Serotyping of L. borgpetersenii isolates identified sero

206 Microbiol 49:3766-3770, 2011) for molecular serotyping of S. flexneri This study was performed by se

207 oratory (Baltimore, MD) for confirmation and serotyping of S. flexneri; one-third of isolates were se

208 Serotyping of Salmonella has been an invaluable subtypin

209 Isolation and serotyping of Salmonella were performed according to the

210 advances in the molecular identification and serotyping of Streptococcus pneumoniae are useful for cu

211 Accurate serotyping of Streptococcus pneumoniae remains important

212 Molecular serotyping of the 450 generated genomes identified a div

213 occal genes, non-culture-based detection and serotyping of upper respiratory tract (URT) samples can

214 iable, a feature used for identification and serotyping of various GAS strains.

215 The PCR serotyping on stool achieved 93% (89% to 96%) sensitivit

216 HLA-A and HLA-B serotyping on URD was provided by the registries.

217 ing Vibrio parahaemolyticus strains involves serotyping or detection methods based on assessment of t

218 ledge about its genetic diversity comes from serotyping or ompA genotyping, no quantitative assessmen

219 Serotyping PCR primers were designed from variable regio

220 We used O:K:H;F serotyping, PCR-based genomic fingerprinting, pulsed-fie

221 The methods employed were somatic O serotyping, PCR-restriction fragment length polymorphism

222 Commonly used subtyping methods, such as serotyping, phage typing, ribotyping, and pulsed-field g

223 nd provides a national reference service for serotyping pneumococcal isolates in England and Wales.

224 In addition to serotyping, pneumococcal isolates causing meningitis wer

225 The technical difficulties of serotyping, primarily in antiserum production and qualit

226 as described recently, identification of PCR serotyping primers would further increase the ease and a

227 efficient enzyme-linked immunosorbent assay serotyping protocol was described recently, identificati

228 lly related pharyngeal isolates by M protein serotyping, pulsed field gel electrophoresis (PFGE), and

229 monocytogenes strains were characterized by serotyping, pulsed-field gel electrophoresis (PFGE), and

230 m in the United States were characterized by serotyping, pulsed-field restriction analysis, ribotypin

231 However, serotyping reagents are not widely available, and many i

232 yped, and some may cross-react with multiple serotyping reagents.

233 35B, one isolate failed to bind to critical serotyping reagents.

234 Here we report, from the 15 serotyping reference strains, the DNA sequences of the l

235 o led to replacement with new strains; thus, serotyping remains important for vaccine-related disease

236 We propose that real-time PCR serotyping represents an attractive alternative to curre

237 ontrast to Ab responses to whole flagella (H serotyping), responses to flagellin monomers displayed o

238 served complete concordance between capsular serotyping results and wciP pyrosequencing among 210 iso

239 aluated the concordance between pneumococcal serotyping results by latex agglutination, whole-genome

240 , rendering them dysfunctional; inconclusive serotyping results due to nonspecific cross-reactions; o

241 Serotyping results were consistent between latex aggluti

242 ere analyzed by mPCR and compared to the IHA serotyping results with 90% concordance (excluding those

243 sis and thus likely represent false-positive serotyping results.

244 luster analysis and compared to conventional serotyping results.

245 or certain isolates with ambiguous or non-H7 serotyping results.

246 es not included in the PSGS yielded negative serotyping results.

247 Serotyping revealed that 16 of 18 ABPA patients were eit

248 is study was to evaluate a real-time PCR for serotyping S. flexneri and to use whole-genome sequencin

249 resolve discrepancies in slide agglutination serotyping (SAST) results from state health departments

250 e biotyped, serotyped by slide agglutination serotyping (SAST), and evaluated using PCR capsule typin

251 overing all serotypes, with both the current serotyping scheme and the standardised EnteroBase core-g

252 This serotyping scheme has led to the definition of over 2500

253 We describe an adaptation of the Penner serotyping scheme in which passive hemagglutination has

254 By contrast, the serotyping scheme is affected by horizontal gene transfe

255 taining the integrity of the Kauffmann-White serotyping scheme, thus providing backwards-compatible e

256 i is the major serodeterminant of the Penner serotyping scheme.

257 igens (fliC and fljB) of the Kauffmann-White serotyping scheme.

258 fied antigenic determinants for serogroup 11 serotyping sera and highlight the idea that conventional

259 We also show that serotyping sera and monoclonal antibodies specific for 9

260 We examined the binding of serotyping sera to serogroup 11 polysaccharides by using

261 A combination of ribotyping and serotyping showed that two bovine isolates were indistin

262 r laboratories quality reagents suitable for serotyping strains of salmonellae.

263 Serotyping Streptococcus pneumoniae is a technique gener

264 In the course of large-scale serotyping studies in which fluorescent antibody stainin

265 Data developed from mouse serotyping studies performed >45 years ago, and multiloc

266 previously devised monoclonal antibody-based serotyping system (346 CS6As compared).

267 Our serotyping system can identify not only all the serotype

268 antigenic component of the classical Penner serotyping system distinguishing Campylobacter into >60

269 acterized a rapid semiautomated pneumococcal serotyping system incorporating a pneumococcal lysate pr

270 sibility of developing an improved molecular serotyping system, which would greatly assist diagnosis

271 Serotyping the organism is important in studying the epi

272 In comparison with conventional F serotyping, the assay was extremely sensitive and specif

273 For E. coli, based on serotyping, the ExPEC4V and ExPEC10V vaccines under inve

274 olates have traditionally been classified by serotyping, the serologic identification of two surface

275 exneri WGS data provided both genome-derived serotyping, thus supporting backward compatibility with

276 e used multilocus sequence typing (MLST) and serotyping to build a phylogenetic framework for pneumoc

277 polymerase chain reaction-based assays and O serotyping to define ExPEC-associated traits were perfor

278 rapid, sensitive, and specific screening and serotyping tools for epidemiological studies of dengue v

279 Traditional methods of serotyping using culture and serum neutralization are ti

280 outine analysis of Listeria monocytogenes by serotyping using traditional agglutination methods is li

281 According to serotyping, virulence genotyping, and random amplified p

282 fied by UAD and by Quellung reaction and PCR serotyping was 70/86 (81.4%).

283 Cytomegalovirus (CMV) serotyping was assessed by enzyme-linked immunosorbent a

284 Serotyping was done by latex agglutination and genome se

285 h conventional serotyping and found that PCR serotyping was nearly as discriminatory as conventional

286 Since viral titers were too low, complete serotyping was not possible.

287 was nearly as discriminatory as conventional serotyping was.

288 For genomic serotyping, we applied the PneumoCaT pipeline to whole-g

289 For phenotypic serotyping, we used a 13-valent latex kit (Statens Serum

290 Streptococcus pneumoniae carriage and serotyping were assessed using culture-based methods (Qu

291 gens in the United States, serosubtyping and serotyping were done on 444 NMSB strains isolated in the

292 n the well-established utility of Salmonella serotyping when integrated into a platform of WGS-based

293 nzyme-linked immunosorbent assay (ELISA) for serotyping which is sensitive and specific compared to t

294 In addition to serotyping, which is subject to false-positive results,

295 We compared the data from PCR serotyping with conventional serotyping and found that P

296 ples and/or rectal swabs underwent molecular serotyping with cycle threshold (Ct) values provided by

297 d pipelines: Illumina sequencing followed by serotyping with PneumoCaT, SeroBA, and Pathogenwatch vs

298 59 [85.5%] of 69 isolates) was identified by serotyping (with 85.5% of the isolates being 19A or 19F)

299 This method will find utility in high-volume serotyping work.

300 g sera and expertise needed for conventional serotyping yet has the modest equipment necessary for DN