ライフサイエンスコーパス: serum-free

1 ollagen accumulation and PAI-1 expression in serum-free, 0.1% ITS+ culture; larger increases in these

2 murine embryonic stem cells were adapted to serum-free 2i medium, leading to a significant decrease

3 urements from mESCs cultured in serum/LIF or serum-free 2i/LIF conditions.

4 ethods by employing the secretion pathway of serum-free adapted human suspension cell lines, such as

5 (but which contains fetal calf serum), and a serum-free alternative, M2 (melanocyte medium), were exp

6 In serum, free and conjugated BPA were detected at sub ng/m

7 orneal stroma and in keratocytes cultured in serum-free and insulin-supplemented media.

8 Here, we have developed a defined, serum-free and low cell-density differentiation program

9 HCR-TG-FRET showed similar performance for serum-free and serum-containing samples without the use

10 We describe here a serum-free, artificial thymic organoid (ATO) system that

11 sk is found at doses that yield undetectable serum free BPA.

12 Serum free-BPA and BPA-glucuronide were quantitated in s

13 associated with a significant deficiency in serum free carnitine (43%; P < 0.001) and elevated acyl

14 protamine (HPF) nanocomplexes were stable in serum-free cell culture medium.

15 is our recommendation that researchers adopt serum-free cell culture methods to reduce animal use in

16 age of bovine stifle joints were cultured in serum-free chemically defined medium.

17 stifle joints and cultured as monolayers in serum-free chemically defined medium.

18 g/mL bFGF, 10 ng/mL PDGF) supplementation of serum-free chondrogenic expansion medium enhances the po

19 Analysis of serum-free conditioned media from three breast cancer ce

20 nalysis was used to detect secreted BMP-4 in serum-free conditioned media of ONH cells and in human O

21 n microvascular endothelial cells (HMECs) in serum-free conditioned medium of glioblastoma cells tran

22 y tandem mass spectrometry, we identified in serum-free conditioned medium of neuroblastoma cells sev

23 stablish these cultures in the fully defined serum-free conditioned medium that is required to sustai

24 protein in HEK293 cells and purified it from serum-free, conditioned medium.

25 ssing prorenin was generated and grown under serum free conditions in a hollow fiber bioreactor.

26 liferation of Chinese hamster ovary cells in serum-free conditions (in the absence of vitronectin).

27 ere observed in SK-N-SH cells under serum or serum-free conditions and in WT or Atg5(-/-) mouse embry

28 Protection from apoptosis under serum-free conditions correlated with bcl-2 transcriptio

29 Moreover, studies conducted under serum-free conditions demonstrated that specific nylon-3

30 entiation, human aortic SMC were cultured in serum-free conditions for 10 days.

31 Serum-free conditions greatly enhanced the sensitivity o

32 ricyte-induced tube maturation under defined serum-free conditions in 3-dimensional matrices.

33 ed with an increase of PFU, especially under serum-free conditions in the later stages of the viral r

34 phosphatidylinositol 3-kinase activity under serum-free conditions in vitro.

35 ly showed primary keratocytes cultured under serum-free conditions to secrete matrix similar to that

36 h replicating P. aeruginosa (strain PA01) in serum-free conditions was developed, and the influence o

37 F-beta, IL-1beta and IL-6, IL-21 or IL-23 in serum-free conditions were necessary and sufficient to i

38 Primary human corneal keratocytes under serum-free conditions were used as a model of corneal st

39 ctive agonist Y6AII induces proliferation in serum-free conditions whereas the AT2R-specific antagoni

40 demonstrated that cell death occurred under serum-free conditions, and that GDNF significantly reduc

41 ere expanded as monolayers, aggregated under serum-free conditions, and transplanted into normoglycem

42 GDNF also stimulated cell proliferation in serum-free conditions, as assessed by the BrdU incorpora

43 fferentiated in the presence of activin A in serum-free conditions, mouse embryonic stem cells effici

44 Even under serum-free conditions, NP cells did not induce autophagy

45 and the cytokines IL-3, IL-9, and IL-6 under serum-free conditions, or by KITLG alone in the presence

46 ibition during staurosporine induction, with serum-free conditions, results in down-regulation of APP

47 ese spheroids were cultured for 3-4 weeks in serum-free conditions, sustaining their phase I enzyme e

48 In serum-free conditions, these cells expressed IalphaI, le

49 dent three-dimensional coculture systems and serum-free conditions, we compared the ability of estrog

50 ut not AdipoR1 alone, supported growth under serum-free conditions, while simultaneous expression of

51 th the lowest IC(50) values below 10nM under serum-free conditions.

52 nd resumes invasion by the tumor cells under serum-free conditions.

53 acquisition of the ability to proliferate in serum-free conditions.

54 d progenitors to neural stem cells (NSCs) in serum-free conditions.

55 enged with endotoxin-free MSU crystals under serum-free conditions.

56 phagic death, allowing prolonged survival in serum-free conditions.

57 in response to serum and estrogen, and under serum-free conditions.

58 oblasts with organotypic functionality under serum-free conditions.

59 m cell-derived cardiomyocytes under defined, serum-free conditions.

60 langerin(high)-expressing cells but only in serum-free conditions.

61 D PHH spheroid system in chemically-defined, serum-free conditions.

62 activities when compared with high-glucose, serum-free conditions.

63 ended periods when compared to high-glucose, serum-free conditions.

64 FN initiates EMT under serum-free conditions; this response is partially revers

65 ed in primary cultures under wholly defined (serum-free) conditions that we developed for short-term

66 romatin modifying agents and cytokines under serum-free-conditions significantly enhances engraftable

67 nd retinal ciliary bodies were maintained in serum-free culture and genetically modified by electropo

68 n that it uses entirely defined, feeder- and serum-free culture conditions and produces very consiste

69 ue macrophage populations, we have optimized serum-free culture conditions to permit robust survival

70 Supplementing defined stroma- and serum-free culture conditions with recombinant DPT prote

71 Remarkably, under hematopoietic serum-free culture conditions, hematopoietic outgrowth o

72 od after high-salt intake can potentiate, in serum-free culture conditions, the differentiation of fr

73 ach to cultivate primary human ECs (hECs) in serum-free culture conditions.

74 pendently increased viable cell number under serum-free culture conditions.

75 uripotent stem (iPS) cells that uses defined serum-free culture conditions.

76 A serum-free culture containing SCF, TPO, FGF-1, angiopoie

77 studies, hTCEpi cells were cultured in KGM-2 serum-free culture media containing 0.15 mM calcium.

78 IL-9 was detected in serum-free culture medium harvested from ALK+ ALCL-cell

79 ature bovine articular cartilage explants in serum-free culture medium.

80 ntiation occurred in a clinically applicable serum-free culture model and was not accompanied by apop

81 d decades ago, a long-term, feeder cell- and serum-free culture system recapitulating murine epiderma

82 Adapting a line to a serum-free culture system resulted in additional epigene

83 ratinocytes cultured in a chemically defined serum-free culture system, devoid of animal-derived feed

84 In a minimal, serum-free culture system, the synthetic GC dexamethason

85 Using a serum-free culture system, we discovered that IGF2 can s

86 m mouse ciliary epithelium and maintained in serum-free culture were genetically modified by electrop

87 ce of human serum, which was not observed in serum-free culture.

88 ar cells and HBEGF promote their survival in serum-free culture.

89 In this study, we report that serum-free cultured human monocyte-derived DCs after TLR

90 Serum-free cultured rabbit corneal keratocytes and TGFbe

91 human brains and to maintain these cells in serum-free cultures.

92 e colonic or proximal intestinal lineages in serum-free defined conditions.

93 The medium was exchanged for a serum-free defined medium containing corresponding stero

94 om human prenatal donor eyes and cultured in serum-free defined medium containing the commercially fo

95 f total cell RNA from cultures maintained in serum-free defined medium for up to 190 days.

96 RGCs were cultured in serum-free defined medium in 96-well plates.

97 In a serum-free defined medium, OLG undergo apoptosis and dif

98 expansion for >150 population doublings in a serum-free, defined medium and with a doubling time of a

99 ynthemax(R) Surface, for culture of hMSCs in serum-free, defined medium.

100 Through the use of this serum-free, defined system, we demonstrate that pericyte

101 A serum-free differentiation (SFD) medium completely lacki

102 arious developmental stages of a feeder- and serum-free differentiation method and show that the larg

103 Here we report an optimized serum-free differentiation protocol to efficiently produ

104 epared from human donor eyes and cultured in serum-free DMEM.

105 Control cultures were exposed only to serum-free DMEM.

106 All cytokines were diluted in serum-free DMEM.

107 RCKs cultured in serum-free DMEM/F12 without frequent changes of medium m

108 CM was collected from 72 h serum-free DPSC cultures and neurotrophic factors; nerve

109 As a result of this muscle diversion, serum-free FA and ketone bodies rose much less after fas

110 mediated by beta-arrestin1, but lowering of serum free fatty acid levels is not.

111 nicotinic acid, although the improvement in serum free fatty acid levels was similar to that observe

112 visceral fat were positively correlated with serum free fatty acid levels, suggesting that collagen a

113 temperature at 4 degrees C, despite elevated serum free fatty acid levels.

114 ontrol mice showed a significant increase in serum free fatty acids (FFAs) and decrease in subcutaneo

115 licing with the levels of plasma glucose and serum free fatty acids (FFAs) in three independent studi

116 Serum free fatty acids (FFAs) profile is highlighted in

117 Serum free fatty acids increased by 10 micromol/L in the

118 cantly reduced, medium chain fatty acids and serum free fatty acids were elevated.

119 es of FGF21 improve glucose tolerance, lower serum free fatty acids, and lead to weight loss in obese

120 ve lipase activity results in a reduction of serum free fatty acids, leading to improved peripheral i

121 that is associated with increasing level of serum-free fatty acids and worsening glycemic control.

122 This was associated with increased serum free-fatty acids, reduced total white adipose, and

123 the COMECS protocol, using a feeder-free and serum-free (FFSF) culture system.

124 Using serum-free fully-defined culture medium formulations, we

125 The aNSCs proliferated when cultured in serum-free growth media on peptide-modified vmIPNs with

126 t were preservable for at least 3 weeks in a serum-free, high-calcium medium and, with brief trypsin/

127 lity of HCEC aggregates was preservable in a serum-free, high-calcium, but not low-calcium, medium fo

128 factors and cytokines, in combination with a serum-free, hormonally defined medium (HDM) tailored for

129 CL wear) before and after PA exposure and in serum-free human corneal epithelial cell culture (hTCEpi

130 referred to as FGRS), and then propagated on serum-free instructive vascular niche monolayers to indu

131 elial cells and cell line were cultured in a serum-free keratinocyte medium and DMEM/F12 medium conta

132 r these clinical programs and that comprises serum-free Kubota's Medium (KM) supplemented with 10% di

133 decreased ex vivo tissue contraction down to serum-free levels.

134 tein electrophoresis with immunofixation and serum free light chain (FLC) analysis were performed on

135 The serum free light chain (FLC) assay quantitates free kapp

136 g multiple myeloma (SMM), as detected by the serum free light chain (FLC) assay, indicates an increas

137 ospectively evaluated serial paraprotein and serum free light chain (FLC) measurements and found that

138 The median IgM paraprotein was 8 g/L and serum free light chain (FLC) ratio was abnormal in 77 (8

139 rapy (bortezomib-based in 58%), 67% achieved serum free light chain (FLC) response, including very go

140 Serum free light chain (sFLC) assays are well establishe

141 Alternatively, serum free light chain (sFLC) measurements have better s

142 Serum free light chain (sFLC) ratio was 0.0001.

143 at the relative clinical influence is of the serum free light chain ratio (sFLCr) and bone marrow (BM

144 sion in myeloma has been defined by a normal serum free light chain ratio (SFLCR) in addition to the

145 Combination of elevated serum free light chain, M-spike, and GEP70 risk score id

146 difference between involved minus uninvolved serum free light chains (dFLC) has been established as a

147 nal B cells from normal B cells, we measured serum free light chains (FLCs).

148 In patients with < 50% reduction of serum free light chains (sFLCs) after 3 cycles, chemothe

149 ed plasma cell dyscrasia (difference between serum free light chains [dFLC]) >180 mg/L as an overall

150 identify gene defects and the measurement of serum free light chains to identify secondary hypogammag

151 s and immunofixation, as well as analyses of serum free light chains, should also be performed to ide

152 phoresis in 21 patients (77.8%), and only by serum free light-chain analysis in 6 patients (22.2%).

153 horesis, immunofixation electrophoresis, and serum free light-chain analysis were performed on all se

154 We did a serum free light-chain assay on all samples with suffici

155 Using serum-free light chain for assessing response, 77% of pa

156 e the need for BM studies; 10% with a normal serum-free light chain ratio had BM plasma cells more th

157 dding a requirement for normalization of the serum-free light chain ratio to negative immunofixation

158 nce in involved amyloidogenic and uninvolved serum-free light chains (dFLC) < 10 mg/L (low dFLC respo

159 Strategies to rapidly remove nephrotoxic serum-free light chains combined with novel antimyeloma

160 yields CD34(+) cells that can be expanded in serum-free liquid culture into large numbers of megalobl

161 o promote survival of neurons cultured under serum-free, low-insulin conditions, with FGF-2 surprisin

162 In a serum-free matrix, the median LOD and LOQ for cytokine p

163 commercial media, such as BalanCD and CHO-S serum-free media (SFM)-II, as well as in a defined serum

164 ectin (VN), or collagen (CL) in supplemented serum-free media alone or with TGF-beta1 or fibroblast g

165 Furthermore, cultured RGC maintained in serum-free media are also C1q and C3 immunoreactive, dem

166 CSp-EMVs were isolated from serum-free media conditioned for 3 days by cardiospheres

167 ult (12 weeks) rat ganglia and maintained in serum-free media containing glucose (10-100 mM) in the p

168 SC were cultured as free-floating pellets in serum-free media for 3 weeks.

169 Serum-free media for CHO-K1 cells require putrescine sup

170 TRA-1-60(-)/SSEA4(-)/SOX1(+) cells grown in serum-free media give rise to multipotent NSCs with an e

171 an pancreatic cells were proliferated with a serum-free media in monolayer cultures through multiple

172 ed marker shows spheroid colony formation in serum-free media in vitro, as well as tumorigenic abilit

173 NA levels in neuroblastoma cells cultured in serum-free media increased after 8 to 16 hours in BDNF.

174 incubation of mouse embryonic fibroblasts in serum-free media induces caspase-3 activation, an effect

175 Exposing cells to serum-free media on their basolateral side significantly

176 ures of hepatocytes and endothelial cells in serum-free media seeded under 95% oxygen maintain functi

177 results demonstrate that fiber hydrogel and serum-free media synergize to provide an optimal environ

178 Here, we define in serum-free media the minimal factor requirement controll

179 338) with a series of chemically defined and serum-free media to induce formation of posterior foregu

180 Pellet cultures of hCSSC in serum-free media upregulated the expression of keratocyt

181 media and forms neurospheres in supplemented serum-free media was developed from retinal tumors isola

182 NTM5 and GTM3) human TM cells conditioned in serum-free media were incubated in the absence or presen

183 ts before SCT, which were cultured 4 days in serum-free media with hematopoietic growth factors.

184 neration lentiviral vector in FreeStyle 293 (serum-free media) in suspension.

185 ing synthetic multifunctionalized hydrogels, serum-free media, and densely seeded good manufacturing

186 roliferation induced by nonconducting EAG in serum-free media, and EAG increased p38 MAP kinase activ

187 sal-like phenotype in vitro when cultured in serum-free media, and undergoes phenotypic changes consi

188 f complement on CD19 loss was examined using serum-free media, C3- and C5-deficient sera, and a C5-bl

189 n as VEGFA) and dickkopf homolog 1 (DKK1) in serum-free media, human embryonic-stem-cell-derived embr

190 ated dishes and cultured for up to 7 days in serum-free media, platelet derived growth factor BB (PDG

191 However, culture in serum-free media, presence of nerve growth factor, or ad

192 growth factor and hematopoietic cytokines in serum-free media, yielded a precursor population enriche

193 and then TGF-beta2 was added for 24 hours in serum-free media.

194 ined, even when these cells were cultured in serum-free media.

195 fection using standard cell culture media or serum-free media.

196 ter monolayer expansion, and after 1 week in serum-free media.

197 n synthase kinase-3 (GSK3) inhibitor, and in serum-free media.

198 WNT signaling, cell growth, and survival in serum-free media.

199 After challenge in serum free medium, CPCs treated with the 3 microRNA cock

200 division (P = 0.01), increased migration in serum-free medium (72 +/- 18 migrated cells versus 33 +/

201 Rat aortic ECs (n=30) or serum-free medium (controls; n=29) were seeded endovascu

202 ed cell cycle entry by >5-fold compared with serum-free medium (from 13.5 to 78%), but at the single

203 inar-like phenotype when the [Ca(2+)] in the serum-free medium (keratinocyte growth medium, KGM) was

204 +) cells, Dox(-)cells, or an equal volume of serum-free medium after surgically induced myocardial in

205 thus containing a mixture of them all, or in serum-free medium alone.

206 -derived stem cells to naive pluripotency in serum-free medium alone.

207 ostate development, fetal UGSs were grown in serum-free medium and 5 alpha dihydrotestosterone (DHT)

208 ptides prepared from M. arthritidis grown in serum-free medium and also from a 41-kDa known bioactive

209 and cantilever substrates with a DETA SAM, a serum-free medium and refined culture techniques.

210 as the spherogenicity of single CRC cells in serum-free medium and the size of the side population (S

211 HCECs were cultured in serum-free medium and treated with 0 or 10 ng/mL TGFB1 o

212 Transformed RGC-5 cells were cultured in serum-free medium and were treated with 0.5 muM to 2.0 m

213 0 and Rdh16 mRNA in HepG2 cells incubated in serum-free medium by inhibiting transcription and destab

214 by substratum-independent pellet culture in serum-free medium containing ascorbate.

215 A serum-free medium containing SCF, TPO, and FGF-1 or Flt3

216 m of hyaluronans and Kubota's medium (KM), a serum-free medium designed for endodermal stem/progenito

217 T. denticola grown in a serum-free medium did not exhibit increased susceptibili

218 Over more than 3 weeks, cells grown in serum-free medium expanded more than 800,000-fold, and 8

219 tes were incubated in an amino acid-free and serum-free medium for 3 hours prior to onset of anoxia.

220 endothelial cells (bAECs) were incubated in serum-free medium for 6 h before addition of 50 nmol/l f

221 of sEVs secretion from TK6 cells cultured in serum-free medium for a culturing period from 1 to 48 h.

222 re isolated from human tracheas and grown in serum-free medium for one week.

223 CD133+ FACS-sorted cells cultured in serum-free medium form 3-fold more neurospheres than do

224 and 39 + or - 13% in the Dox(+), Dox(-), and serum-free medium groups, respectively (P<0.05 for the d

225 cells within six CRC lines form spheroids in serum-free medium in suspension.

226 orescence protein mice and grown for 48 h in serum-free medium in the presence or absence of Ang II.

227 The addition of exogenous FA in serum-free medium increased oxLDL binding and uptake to

228 TGF-beta1 alone or combined with PDGF-BB in serum-free medium induces a temporally correct expressio

229 ncompatible 3F7.A10 hybridoma cells grown in serum-free medium mounted strong anti-Id responses.

230 were added individually to cells growing in serum-free medium next to controls in medium supplemente

231 This set-up included use of serum-free medium of defined composition with supplement

232 oblasts (HCFs) were cultured in supplemented serum-free medium on VN or collagen (CL) with 1 ng/mL tr

233 A serum-free medium revealed two effects of TNFalpha: (1)

234 Nanog enables somatic cell reprogramming in serum-free medium supplemented with LIF, a culture condi

235 When grown in a serum-free medium supplemented with starch, M. arthritid

236 tant was able to grow to a higher density in serum-free medium than the wild-type strain, mimicking t

237 ent characteristics (HRA-19) and developed a serum-free medium that induces endocrine, mucous and abs

238 st-selection HMEC, that is, cells grown in a serum-free medium that overcame stasis via silencing of

239 rugs--ferumoxytol, heparin and protamine--in serum-free medium to form self-assembling nanocomplexes

240 moxifen or dexamethasone in phenol red-free, serum-free medium to measure the steady-state levels of

241 olyethylene glycol hydrogels and cultured in serum-free medium to model cellular interactions within

242 -human CD28 antibodies for 72 hours in AIM V serum-free medium to obtain T cell-conditioned medium, f

243 uman CB-derived CD34+ cells were cultured in serum-free medium together with SCF, TPO, FGF, with or w

244 CD36-mediated uptake in serum-free medium was very low but greatly increased whe

245 d from rabbit corneal stroma and plated in a serum-free medium were treated with FGF-2/heparin or TGF

246 unstimulated T cells which were cultured in serum-free medium with circadian clock reporter systems.

247 dipose-derived stem/stromal cells (ADSCs) in serum-free medium with efficiencies >90%.

248 They grew as neurospheres in serum-free medium with epidermal growth factor and fibro

249 om collagenase A digestion were preserved in serum-free medium with low or high calcium for up to 3 w

250 The model uses serum-free medium, a nonbiological substrate N-1[3(trime

251 eatment with HES1 shRNA, cell aggregation in serum-free medium, and a mixture of soluble factors furt

252 Mechanical activation of mTOR occurred in serum-free medium, indicating that it is independent of

253 In serum-free medium, most antibiotics (except polymyxins)

254 rom rabbit corneal stroma, and cultured in a serum-free medium, pretreated or not treated with JNK in

255 They clonogenically expand on plastic and in serum-free medium, tailored for endodermal progenitors,

256 so inhibited the growth of Hep3BX cells in a serum-free medium, which correlated with depressed level

257 acrine cells were cultured at low density in serum-free medium, with and without peptide trophic fact

258 ng additionally significantly more active in serum-free medium.

259 aline-like translucent cartilage particle in serum-free medium.

260 cells form neurospheres when transferred to serum-free medium.

261 t neurospheres in growth factor supplemented serum-free medium.

262 land epithelial cells and to culture them in serum-free medium.

263 um treated with methyl-beta-cyclodextrin, or serum-free medium.

264 enosine nucleotides cannot support growth in serum-free medium.

265 were cultured in high-density monolayers in serum-free medium.

266 rabbit corneal keratocytes were cultured in serum-free medium.

267 posure to IL-1beta with or without NS-398 in serum-free medium.

268 nd keratocan) were expressed and secreted in serum-free medium.

269 an astrocyte feeder layer and maintained in serum-free medium.

270 x 10(11)M) with or without NS-398 (10 nM) in serum-free medium.

271 ly become mature podocytes by culturing in a serum-free medium.

272 ((3)H-adenosine) in low-physiologic-glucose serum-free medium.

273 ccumulation, promoting cell proliferation in serum-free medium.

274 he antiproliferative effects of rapamycin in serum-free medium.

275 nonadjuvanted, isologous mAbs purified from serum-free medium.

276 sa association with both substrates, only in serum-free medium.

277 wild-type mice were avulsed and cultured in serum-free medium.

278 Our approach provides a serum-free method for differentiation and long-term main

279 The current serum-free nasal polyp organ culture model allows physio

280 In serum-free neuronal differentiation media, a peak level

281 roma by collagenase digestion were plated in serum-free or insulin-, bFGF/heparin sulfate (HS)-, TGF-

282 TGF-beta1 or -2 up-regulates dye coupling in serum-free primary cultures of chick lens epithelial cel

283 Using serum-free primary cultures of chick lens epithelial cel

284 and contact-independent growth and promoted serum-free proliferation of human cells.

285 Here we present an efficient, serum-free protocol for directed differentiation of huma

286 the PSM-like cells, providing an efficient, serum-free protocol for the generation of striated, cont

287 Importantly, using a serum-free protocol, we successfully generated insulin-p

288 free media (SFM)-II, as well as in a defined serum-free, putrescine-containing medium for at least 9

289 why necrotizing infections mainly develop in serum-free spaces (eg, pulmonary alveoli) and open optio

290 ogenitors can be expanded under nonadherent, serum-free, sphere-forming conditions.

291 a that can form nonadherent melanospheres in serum-free stem cell medium, mimicking aggressive malign

292 MEMO and NEUMO, and an antioxidant rich and serum free supplement called SOS.

293 ned a new neuronal medium (BrainPhys basal + serum-free supplements) in which we adjusted the concent

294 in a completely defined, growth factor- and serum-free system by temporal modulation of regulators o

295 his study was conducted to develop a simple, serum-free system to propagate and sustain human RPE in

296 decrease 1.89 mU/L, 1.39-2.39; p<0.0001) and serum free T(4) concentrations decreased from 9.5 pmol/L

297 mature human endothelial cells cultured in a serum-free three-dimensional matrix composed of a mixtur

298 In nonhyperthyroid cats, serum free thyroxine (fT(4)), total T(4) (TT(4)), total

299 mentation did not affect blood lymphocyte or serum free thyroxine concentrations.

300 Serum free thyroxine levels may be below the reference r