ライフサイエンスコーパス: serum-free culture

1 ce of human serum, which was not observed in serum-free culture.

2 ar cells and HBEGF promote their survival in serum-free culture.

3 stimulated for varying times up to 72 hr in serum-free culture.

4 or cell proliferation and differentiation in serum-free culture.

5 le sca-1(+)/c-kit(+)/Gr-1(-) marrow cells in serum-free cultures.

6 human brains and to maintain these cells in serum-free cultures.

7 ny formation in serum-containing, but not in serum-free, cultures.

8 nd retinal ciliary bodies were maintained in serum-free culture and genetically modified by electropo

9 m albumin secretion was increased by 350% in serum-free cultures and by 166% in serum-containing cult

10 -term urea secretion was increased by 79% in serum-free cultures and by 76% in serum-containing cultu

11 me P450IA1 activity was increased by 140% in serum-free cultures and by 820% in serum-containing cult

12 We added radiolabeled scHGF to serum-free cultures and confirmed that tcHGF was being g

13 nsistently across donors in both widely used serum-free cultures and recently reported chemically def

14 donors, were treated with a 110-kDa FN-f in serum-free culture, and expression of various cytokine g

15 ied astrocytes, purified APCs rapidly die in serum-free culture but can be saved by basic fibroblast

16 optic nerve and stimulated to proliferate in serum-free culture by PDGF.

17 n that it uses entirely defined, feeder- and serum-free culture conditions and produces very consiste

18 ue macrophage populations, we have optimized serum-free culture conditions to permit robust survival

19 Supplementing defined stroma- and serum-free culture conditions with recombinant DPT prote

20 Remarkably, under hematopoietic serum-free culture conditions, hematopoietic outgrowth o

21 od after high-salt intake can potentiate, in serum-free culture conditions, the differentiation of fr

22 NAs was assessed, after ET-1 treatment under serum-free culture conditions, with both a formazan assa

23 ach to cultivate primary human ECs (hECs) in serum-free culture conditions.

24 pendently increased viable cell number under serum-free culture conditions.

25 uripotent stem (iPS) cells that uses defined serum-free culture conditions.

26 tress, dependent on the transfection time in serum-free culture conditions.

27 A serum-free culture containing SCF, TPO, FGF-1, angiopoie

28 blood precursors with immobilized Delta-1 in serum-free cultures containing fibronectin and hematopoi

29 Serum-free culture filtrates of six Candida species and

30 he long-term survival of most of the SMNs in serum-free culture for 3 weeks.

31 interleukin-1alpha (IL-1alpha), and IL-3 in serum-free cultures for as few as 48 hours, the number o

32 In serum-free cultures, however, LIF is insufficient to blo

33 In this study, we report that serum-free cultured human monocyte-derived DCs after TLR

34 optic nerve can proliferate indefinitely in serum-free culture if prevented from differentiating; va

35 gic and phenotypic features were observed in serum-free cultures in the presence of FN/PMA.

36 onor corneas of various ages and grown under serum-free (cultured keratocytes) or serum-added (cornea

37 studies, hTCEpi cells were cultured in KGM-2 serum-free culture media containing 0.15 mM calcium.

38 antities of the recombinant NC1 protein from serum-free culture media.

39 Here we show a human-serum-free culture medium (HSF), which was a mixture of

40 Factor VIII expression was performed in serum-free culture medium and in the absence of exogenou

41 medium containing 15% newborn calf serum, in serum-free culture medium containing either activated TG

42 IL-9 was detected in serum-free culture medium harvested from ALK+ ALCL-cell

43 n a defined, essential-fatty-acid-deficient, serum-free culture medium without a feeder layer, that c

44 ature bovine articular cartilage explants in serum-free culture medium.

45 ll development by in vitro colony assay in a serum-free culture medium.

46 ntiation occurred in a clinically applicable serum-free culture model and was not accompanied by apop

47 rized hypertrophic responses in low-density, serum-free cultures of neonatal mouse cardiac myocytes a

48 Treatment of serum-free cultures of osteoblast clones derived from IG

49 on analyses of acutely purified astrocytes, serum-free cultures of primary astrocytes, and xenograft

50 gh cloning frequencies in stromal cell-free, serum-free cultures, permitting this analysis of direct

51 Serum-free cultured rabbit corneal keratocytes and TGFbe

52 Studies using micromanipulation and serum-free culture showed that the effects of sTPOR and

53 In serum-free culture, supplementation of the medium with I

54 In this study, a serum-free culture system and a transplantation assay fo

55 self-renewal of mouse SSCs and established a serum-free culture system for their proliferation in vit

56 ll (DC) differentiation, we have developed a serum-free culture system in which human CD14+ periphera

57 d decades ago, a long-term, feeder cell- and serum-free culture system recapitulating murine epiderma

58 Adapting a line to a serum-free culture system resulted in additional epigene

59 We used a serum-free culture system to study the effect of RA on c

60 ratinocytes cultured in a chemically defined serum-free culture system, devoid of animal-derived feed

61 In an SCF-dependent serum-free culture system, LPA (2.5-10 microM) increased

62 In a minimal, serum-free culture system, the synthetic GC dexamethason

63 Using a serum-free culture system, we discovered that IGF2 can s

64 SF), and exhibit autonomous proliferation in serum-free cultures that is inhibited by anti-IL-3 and a

65 Compared with serum-free cultures, the use of serum resulted in an ave

66 urified populations of CFU-MK were tested in serum-free cultures, these results suggest that SDF-1 di

67 uring marrow CD34+ cells for 7 to 10 days in serum-free cultures was able to expand CFU-Meg approxima

68 8.5 p.c.-ectoplacental cone (EPC) explant in serum-free culture, we have found parathyroid hormone-re

69 m mouse ciliary epithelium and maintained in serum-free culture were genetically modified by electrop

70 ail, we treated neonatal cardiac myocytes in serum-free culture with a combination of the macrophage-

71 er, TKT loss was not induced by treatment of serum-free cultures with a third serum cytokine, transfo

72 TKT loss was induced by treatment of serum-free cultures with the serum cytokines platelet-de

73 TPO in serum-free cultures without any other exogenously added