ライフサイエンスコーパス: serum-free media

1 s and their ability to grow in suspension or serum free media.

2 with FBS for effective normalization of the serum free media.

3 ined, even when these cells were cultured in serum-free media.

4 fection using standard cell culture media or serum-free media.

5 ter monolayer expansion, and after 1 week in serum-free media.

6 hneider insect (S2) cells, and purified from serum-free media.

7 and at 5 DPS media were replaced with fresh serum-free media.

8 n synthase kinase-3 (GSK3) inhibitor, and in serum-free media.

9 olated rabbit keratocytes plated in defined, serum-free media.

10 ic phenotype and appearance when cultured in serum-free media.

11 tes also cleave IgG in both growth media and serum-free media.

12 roliferation and motility when maintained in serum-free media.

13 st model, cells were grown in low density on serum-free media.

14 WNT signaling, cell growth, and survival in serum-free media.

15 ptosis and that apoptosis was potentiated in serum-free media.

16 and 100 micrograms/ml) for up to 48 hours in serum-free media.

17 m is 22 days) were incubated in hormone- and serum-free media.

18 ry explants were generated and maintained in serum-free media.

19 or patient-derived tumor organoids formed in serum-free media.

20 and then TGF-beta2 was added for 24 hours in serum-free media.

21 al cells results in sustained cell growth in serum-free media, a predisposition to develop hyperplasi

22 ectin (VN), or collagen (CL) in supplemented serum-free media alone or with TGF-beta1 or fibroblast g

23 proteoglycans synthesized by keratocytes in serum-free media also more closely resembled that of ker

24 s can be continuously propagated in defined, serum-free media and 5% oxygen without specialized growt

25 d (G12V)Ha-ras or (Q61K)N-ras proliferate in serum-free media and have constitutive MAPK activity.

26 atal neurons grown in low density culture on serum-free media and in the absence of glia die within 3

27 -alpha) in fetal bovine serum-containing and serum-free media and were analyzed by Wright's stain for

28 sham-operated rats, cultured on Matrigel in serum-free media, and briefly exposed to rat cytomegalov

29 ing synthetic multifunctionalized hydrogels, serum-free media, and densely seeded good manufacturing

30 roliferation induced by nonconducting EAG in serum-free media, and EAG increased p38 MAP kinase activ

31 sal-like phenotype in vitro when cultured in serum-free media, and undergoes phenotypic changes consi

32 Furthermore, cultured RGC maintained in serum-free media are also C1q and C3 immunoreactive, dem

33 well tissue culture plates and maintained in serum-free media at 37 degrees C.

34 ly isolated chondrocytes died when plated in serum-free media at low density on poly-L-lysine, but sh

35 f complement on CD19 loss was examined using serum-free media, C3- and C5-deficient sera, and a C5-bl

36 Also, serum-free media conditioned by transiently compressed g

37 CSp-EMVs were isolated from serum-free media conditioned for 3 days by cardiospheres

38 ult (12 weeks) rat ganglia and maintained in serum-free media containing glucose (10-100 mM) in the p

39 her concentrations of PLL (1.5 microg/mL) in serum-free media during initial FE-PLL complex formation

40 l as acutely isolated astrocytes cultured in serum-free media, failed to respond to 5-HT by changes i

41 cubated in either control, acid, or alkaline serum-free media for 12 h.

42 Confluent cells were exposed to serum-free media for 24 h, then incubated in either cont

43 SC were cultured as free-floating pellets in serum-free media for 3 weeks.

44 e agonists/antagonists to their receptors in serum-free media for 5-7 days.

45 Serum-free media for CHO-K1 cells require putrescine sup

46 systematic, species-specific optimization of serum-free media for immortalized lamb muscle cells (ILM

47 uccessful use of an ITS+ Premix-supplemented serum-free media for prolonged islet culture and its com

48 ddition of TGF-beta1 to endothelial cells in serum-free media further potentiated the induction of ap

49 TRA-1-60(-)/SSEA4(-)/SOX1(+) cells grown in serum-free media give rise to multipotent NSCs with an e

50 ultured in gas-permeable bags containing 1-L serum-free media, granulocyte colony-stimulating factor,

51 rapidly increased after exposure of cells to serum-free media, heat shock, or staurosporine.

52 n as VEGFA) and dickkopf homolog 1 (DKK1) in serum-free media, human embryonic-stem-cell-derived embr

53 an pancreatic cells were proliferated with a serum-free media in monolayer cultures through multiple

54 ed marker shows spheroid colony formation in serum-free media in vitro, as well as tumorigenic abilit

55 neration lentiviral vector in FreeStyle 293 (serum-free media) in suspension.

56 NA levels in neuroblastoma cells cultured in serum-free media increased after 8 to 16 hours in BDNF.

57 The serum-free media-induced decrease in permeability was co

58 incubation of mouse embryonic fibroblasts in serum-free media induces caspase-3 activation, an effect

59 TGF-beta1 to vascular smooth muscle cells in serum-free media inhibited apoptosis.

60 Culturing human islets in Memphis serum-free media (M-SFM) is associated with excellent po

61 In defined, serum-free media, NHBEs undergo mucosecretory differenti

62 Exposing cells to serum-free media on their basolateral side significantly

63 However, when cells were grown with either serum-free media or at high densities, CDK2 levels decli

64 and express five-fold fewer IGF-IRs, die in serum-free media or following exposure to metabolic stre

65 In serum-free media, p50E4F accelerated E1A-induced apoptos

66 ated dishes and cultured for up to 7 days in serum-free media, platelet derived growth factor BB (PDG

67 However, culture in serum-free media, presence of nerve growth factor, or ad

68 o the apoptotic effect of PI3K inhibition in serum-free media, reflecting the heterogeneous nature of

69 ures of hepatocytes and endothelial cells in serum-free media seeded under 95% oxygen maintain functi

70 ent of cells with tyrphostin AG879 prevented serum-free media (SFM) induction of cell proliferation.

71 The development of serum-free media (SFM) is critical for advancing the sca

72 commercial media, such as BalanCD and CHO-S serum-free media (SFM)-II, as well as in a defined serum

73 were cultured in parallel in (A) CMRL + ITS (serum-free media; SFM) or (B) CMRL +10% fetal bovine ser

74 Growth in either serum-containing or serum-free media supplemented with GM-CSF and IL-4 yield

75 results demonstrate that fiber hydrogel and serum-free media synergize to provide an optimal environ

76 Here, we define in serum-free media the minimal factor requirement controll

77 Several studies employed serum-free media to analyze secreted proteins, but it ha

78 338) with a series of chemically defined and serum-free media to induce formation of posterior foregu

79 Pellet cultures of hCSSC in serum-free media upregulated the expression of keratocyt

80 The f-HICF serum free media was prepared, optimised, and tested wit

81 This increased proportion of KS synthesis in serum-free media was caused by a moderate increase in KS

82 media and forms neurospheres in supplemented serum-free media was developed from retinal tumors isola

83 cells, NGF's ability to promote survival in serum-free media was reduced.

84 NTM5 and GTM3) human TM cells conditioned in serum-free media were incubated in the absence or presen

85 an as keratan sulfate (KS), whereas cells in serum-free media were quiescent, appeared dendritic, and

86 aT-AR) displayed autonomous proliferation in serum-free media when compared with controls (HaCaT-NIE)

87 ts before SCT, which were cultured 4 days in serum-free media with hematopoietic growth factors.

88 growth factor and hematopoietic cytokines in serum-free media, yielded a precursor population enriche