ライフサイエンスコーパス: sever

1 ing almost-separate membrane compartments is severed.

2 tion between previously associated events is severed.

3 ct versus one where the Pd-Ti interaction is severed.

4 physical steps when the covalent linkage is severed.

5 s inside the cleavage furrow were kinked and severed.

6 a flagellum is regenerated after it has been severed.

7 rm elasticity, thereby accelerating filament severing.

8 sence of calcium, enabling actin binding and severing.

9 in at Ser-3 regulates both actin binding and severing.

10 A subsequent study only reported severing.

11 r filament mechanical properties and promote severing.

12 be, kinetochores shortened after microtubule severing.

13 solin is one of the most potent for filament severing.

14 ing with actin filaments in real time during severing.

15 the first ATPase associated with microtubule severing.

16 2 accelerates depolymerization subsequent to severing.

17 neurons without further lowering microtubule severing.

18 ents, and cortactin attenuates this enhanced severing.

19 ilaments by cofilin to a range favorable for severing.

20 hereas contractile fibers are protected from severing.

21 operative cofilin binding and actin filament severing.

22 continuities in filament topology to enhance severing.

23 bulin tails are necessary and sufficient for severing.

24 molecule was sufficient to enhance filament severing.

25 anisms to prevent indiscriminate microtubule severing.

26 preserves filament number despite sustained severing.

27 ffect on cofilin binding and weakly enhances severing.

28 F/cofilin binding, but dramatically enhances severing.

29 In response to severing, a finite gap forms and is repaired by recruitm

30 ed inhibitor bound to spastin, a microtubule-severing AAA protein, and characterize the residues invo

31 he evolutionarily conserved microtubule (MT)-severing AAA-ATPase enzyme Katanin is emerging as a crit

32 Cofilin-mediated severing accelerates the turnover and spatial reorganiza

33 embers of the cofilin/ADF family of proteins sever actin filaments, increasing the number of filament

34 igh concentrations of yeast or human cofilin sever actin filaments, most likely by competing with cof

35 onally conserved proteins that cooperatively sever actin filaments.

36 Recently, MVT was reported to sever actin filaments.

37 A key function of ADF/cofilin is to sever actin filaments.

38 rylation activates host gelsolin, leading to severed actin filaments and disturbed actin dynamics.

39 This cleaved villin fragment severs actin in an unregulated fashion to initiate the e

40 er cooperativity than wild-type cofilin, and severs actin weakly across a broad range of occupancies.

41 hanges in endocytic trafficking, microtubule severing, actin clearance, and the physical sealing of t

42 a motor activity that assists the necessary severing action, but again the underlying mechanics is n

43 ffinities, and quantified the actin filament severing activities of human Cof1, Cof2, and ADF using i

44 bound phosphomimetic (S3D) cofilin with weak severing activity adopts a unique binding mode that does

45 oth protofilaments, consistent with a higher severing activity at boundaries compared to single cofil

46 We find that free tubulin can inhibit severing activity by interfering with katanin binding to

47 l and activity to deliver the appropriate MT-severing activity during development.

48 In Caenorhabditis elegans, Katanin MT-severing activity is essential for meiotic spindle assem

49 idewalls of single and bundled microtubules, severing activity is restricted to microtubule cross-ove

50 While severing activity might be expected to break down the mi

51 directly targeted katanin and regulated the severing activity of katanin, which cut the cellular mic

52 We quantified the microtubule-severing activity of purified MEI-1/MEI-2 complexes corr

53 understood, but partial loss of microtubule-severing activity resulting from inactivating mutations

54 n fluorescence microscopy experiments, MVT's severing activity was negligible.

55 minant-negative fashion to lower microtubule-severing activity, but others have detrimental effects o

56 In addition to its ATP-dependent severing activity, spastin is an ATP-independent regulat

57 istent with a single cofilin having filament-severing activity.

58 cruits spastin to microtubules and modulates severing activity.

59 n after spinal cord injury through its actin-severing activity.

60 tions, independent of microtubule-binding or severing activity.

61 nce of glutamylation for spastin microtubule severing activity.

62 katanin concentration on katanin binding and severing activity.

63 microtubule response to stress by increasing severing activity.

64 ed on viable muscle tissue, and to stimulate severed afferent nerve fibers to provide somatosensory f

65 the nucleus, suggesting that dicentrics are severed after actomyosin ring contraction.

66 tastrophic mode of disassembly, not enhanced severing alone.

67 presented to our emergency department with a sever and sharp left iliac fossa pain.

68 Long-term implant of MOSD to mice with severed and anastomosed C7 nerve is proven to be both sa

69 s while simultanteously being stochastically severed and capped along their lengths, and disassembled

70 ranes, the encircled area of membrane can be severed and internalized within the lumen as a fragment

71 raft formation when the vascular strands are severed and reconnected.

72 form as diffusing oligomers, indicating that severing and debranching are important steps in the disa

73 whose primarily function is to regulate the severing and depolymerization of actin filaments.

74 by phosphorylating cofilin to inhibit actin severing and depolymerization.

75 y ordered pathway to induce highly efficient severing and disassembly of actin filaments.

76 esults establish a theoretical basis for how severing and dynamics together can serve to nucleate new

77 in understanding the molecular mechanisms of severing and growth promotion that provide insight into

78 hat this allosteric network is essential for severing and is a hotspot for HSP mutations.

79 spastin remodels microtubule arrays through severing and its mutation is the most common cause of he

80 on of microtubule lengths in the presence of severing and microtubule dynamic instability.

81 oles in actin dynamics by mediating filament severing and polymerization.

82 vimentin IFs (VIFs) undergo rearrangement by severing and reannealing, but direct subunit exchange wi

83 into individual subunits; they recompose by severing and reannealing.

84 This effect of severing and regrowth by spastin on the microtubule leng

85 n turn recruits AIP1, which rapidly triggers severing and remains bound to the newly generated barbed

86 nt for regulation of the roles of cofilin in severing and stabilizing actin filaments.

87 gth distribution and showed that the rate of severing and the speed of microtubule growth are the dom

88 n tails are the most effective at inhibiting severing, and that detyrosinated alpha-tubulin tails are

89 ough a combination of barbed end elongation, severing, and WH2 motif-mediated depolymerization.

90 ion acts as a rheostat and tunes microtubule severing as a function of glutamate number added per tub

91 f rat (either sex) GCs with apical dendrites severed at different locations.

92 Preferential severing at actin-cofilactin boundaries of buckled filam

93 continuities required for efficient filament severing at boundaries.

94 r hand, it has been suggested that selective severing at microtubule crossovers could facilitate the

95 al between initial binding on a filament and severing at the same location.

96 plored how axotomy changes the physiology of severed axons and adjacent uninjured "bystander" neurons

97 onally recovered at later time points, while severed axons degenerated via alpha/Armadillo/Toll-inter

98 The regrowth of severed axons is fundamental to reestablish motor contro

99 Remarkably, severed axons morphologically preserved by axon death pa

100 NS fiber tracts is accompanied by failure of severed axons to regenerate and results in lifelong func

101 onal activation of different axonal mRNAs as severed axons transition from injury to regenerative gro

102 Following acute neural injury, severed axons undergo programmed Wallerian degeneration

103 active zones are preferentially removed from severed axons within hours after injury and that deprivi

104 in Drosophila ensheathing glia responding to severed axons.

105 ant reduction in the regrowth lengths of the severed axons.

106 scade required for proper glial clearance of severed axons.

107 e protease cascade degraded filamin, thereby severing bonds between the cytoskeleton and tissue facto

108 ction in cofilin occupancy inhibits filament severing, but this hypothesis has remained untested.

109 , is that cross-linked filament networks are severed by cofilin far more efficiently than nonconnecte

110 The mutant filaments are more readily severed by cofilin.

111 p1 does not cap the barbed ends of filaments severed by cofilin.

112 When dendritic microtubules were severed by laser-based microsurgery, we detected equal n

113 er, when the feedback loop is intermittently severed by sensorimotor distractions.

114 nterface increase Ca(2+)-independent F-actin severing by A1-A3, albeit at a lower efficiency than obs

115 py, we show that Crn1 enhances Cof1-mediated severing by accelerating Cof1 binding to actin filament

116 lular actin networks requires actin filament severing by actin-depolymerizing factor (ADF)/Cofilin pr

117 ose a possible mechanism behind the enhanced severing by AIP1.

118 less stable than WT and more susceptible to severing by cofilin.

119 icrotubules by MAP65-1 confers resistance to severing by inhibiting the binding of KTN1 and identifie

120 nse in Arabidopsis thaliana depends on their severing by katanin at crossovers.

121 nt with cooperativity between actin filament severing by myosin-induced forces and by gelsolin.

122 the actin cytoskeleton and on actin filament severing by the regulatory protein cofilin.

123 the biophysical basis of the actin filament severing by these proteins.

124 group and 12/50 (24.0%) each in moderate and sever categories.

125 tration, appreciably enhanced actin filament severing caused by HMM-induced forces at 1 mM MgATP, an

126 and AIP1, which both mediate actin filament severing, contribute to stereocilia length maintenance.

127 protein subunits and cofilin mutations with severing defects, but no genetic interaction with deleti

128 e found that three factors contribute to the severing deficiency of S3D cofilin.

129 Cofilin-1-severing/depolymerization activity is negatively regulat

130 the bud neck, explaining how cytokinesis can sever dicentrics near centromeres.

131 Here the authors study how severed distal axons signal back to the cell body to ind

132 Reduced severing due to microtubule bundling by MAP65-1 correlat

133 atalytic (p60) and regulatory (p80) subunits severs dynamic microtubules to modulate several stages o

134 by regulatory proteins can modulate both the severing efficiency and location along filaments.

135 dges of extracellular matrix that ligate the severed ends of the spinal cord.

136 The latter form a microtubule-severing enzyme complex that regulates microtubule dynam

137 The microtubule-severing enzyme katanin (KTN1) regulates the organizatio

138 Experiments have identified the microtubule-severing enzyme katanin as a central player in controlli

139 trol of cortical microtubule dynamics by the severing enzyme KATANIN became vital when XyGs were pert

140 und that interaction between the microtubule-severing enzyme spastin and the ESCRT protein IST1 at ER

141 on appears to be mediated by the microtubule-severing enzyme spastin, which is dysfunctional in some

142 he hereditary spastic paraplegia microtubule severing enzyme spastin.

143 y the previously uncharacterized microtubule-severing enzyme, Fidgetin-like 2 (FL2), as a fundamental

144 eddine et al. describe a role for a novel MT-severing enzyme, fidgetin-like 2 (FL2), in directional m

145 Microtubule-severing enzymes - katanin, spastin, fidgetin - are rela

146 se results are distinct from those for other severing enzymes and suggest a scheme for regulation of

147 ially regulating the activity of microtubule-severing enzymes and the movement of molecular motors th

148 Microtubule-severing enzymes are critical for the biogenesis and mai

149 Microtubule severing enzymes implement a diverse range of tissue-spe

150 Effective siRNA nanoparticle targeting of MT-severing enzymes offers promise of controlled and target

151 ve been shown to be targeted for removal via severing enzymes or self-repair.

152 owth promotion that provide insight into how severing enzymes shape microtubule networks.

153 er of cellular activities is the microtubule-severing enzymes, yet little is known about how they fun

154 idation of actin allows for cofilin-mediated severing even in the presence of inorganic phosphate.

155 of microtubules, resulting from microtubule-severing events, may play a role in endosomal tubulation

156 plains in quantitative detail the results of severing experiments and use it to make predictions that

157 n is insufficient to describe the results of severing experiments, in which a flagellum is regenerate

158 was associated with activation of the actin severing factor cofilin.

159 tin nucleation and elongation, INF2 can also sever filaments and accelerate their depolymerization.

160 However, Cof2 and ADF severed filaments much more efficiently than Cof1 at bot

161 Cofilin promotes phosphate dissociation and severs filaments, generating new pointed ends and render

162 lands at the same time that rising sea level severed former dispersal routes.

163 aments, many clathrin-coated pits fail to be severed from the membrane, causing accumulation of large

164 tracted by approximately 100 mum after being severed from the neurons, indicating considerable steady

165 ethinus) cvs. Chanee and Monthong fruit were severed from the tree during 14 day intervals, from 10 w

166 ssembly function of Vps4 and the microtubule-severing function of spastin, as well as potentially kat

167 Severing habenular efferents to the IPN, or only those f

168 standing of cooperative binding and filament severing has been impeded by a lack of structural data d

169 s critical to boost ADF/cofilin's ability to sever highly connected filament networks in cells.

170 engthening and healing after the material is severed, ii) bulk property changes are spatially confine

171 ticularly under confinement, emphasizes that severing in cells is likely to be influenced by multiple

172 across neighboring microtubules, modulating severing in trans.

173 n MAP65-1 inhibits KTN1-mediated microtubule severing in vitro.

174 zation via filament disassembly although not severing, in contrast to previous reports.

175 d SUN-domain protein Sad1 remains stable but severs interphase centromere-LINC contacts.

176 We demonstrate that cofilin-mediated severing is a single-timescale mode of stress relaxation

177 Although microtubule severing is fundamental to many dynamic neural processes

178 nts by polymerization, depolymerization, and severing is important for cell locomotion, membrane traf

179 Microtubule severing is important for regulating various aspects of

180 Severing is inhibited at bundled microtubule segments an

181 is for these activities and how they lead to severing is unknown.

182 Thus microtubule severing may not be as reduced as previously assumed in

183 amin displayed the capacity to constrict and sever membrane tubes.

184 re an ancient system that buds membranes and severs membrane necks from their inner face.

185 nzymes spastin, katanin, and fidgetin, which sever microtubule polymers into shorter fragments.

186 ynamics promote microtubule regrowth so that severed microtubule fragments grow, leading to an increa

187 In this work, we used laser ablation to sever microtubules attached to a merotelic kinetochore,

188 a novel M187 spastin isoform that is able to sever microtubules.

189 ing multiple aspects of cellular dynamics by severing microtubules.

190 The AAA ATPase katanin severs microtubules.

191 tase (GTPase) dynamin-related protein (Drp1) severs mitochondria at endoplasmic reticulum (ER)-mitoch

192 ents with similar kinetics, yet Cof2 induced severing much more rapidly than Cof1, decreasing the tim

193 After coaptation of the severed nerve ends, fine wire electrodes were implanted

194 e' of new tissue, which forms to reconnect a severed nerve.

195 ves and from the wound and distal regions of severed nerves.

196 l for neurobiology, used to ablate cells and sever neurites in-vivo.

197 After AIP1 binding to a filament, severing occurred with a delay of 0.7 s.

198 Filament severing occurs preferentially at boundaries between bar

199 ween bare and decorated actin segments where severing occurs preferentially.

200 The severing of actin filaments is mainly achieved by cofili

201 gle-particle tracking reveals that increased severing of actin filaments reduces the spatial confinem

202 Arg greatly potentiates cofilin severing of actin filaments, and cortactin attenuates th

203 as been shown in vitro that cofilin-mediated severing of Arp2/3 actin networks results in the generat

204 ways that cooperatively enable the efficient severing of cytoplasmic connections between dividing dau

205 so, there is growing evidence that decreased severing of microtubules does not fully explain HSP-SPG4

206 ing are likely caused not only by diminished severing of microtubules, but also by neurotoxicity of m

207 karyotic cells by driving the remodeling and severing of microtubules, which are cytoskeletal polymer

208 n motor activity leading to enhanced F-actin severing of possible physiological relevance.

209 nce of the experimentally observed selective severing of the "crossing" microtubule at crossovers, an

210 abscission checkpoint that prevent premature severing of the bridge connecting cells at the end of ce

211 are recruited to the midbody and direct the severing of the intercellular bridge.

212 gon gerardii and several AM fungi in intact, severed or prevented CMNs.

213 branch formation, or cofilin-mediated actin severing or how cortactin influences these reactions of

214 nt branch retraction caused by laser-induced severing or nocodazole-induced microtubule depolymerizat

215 ups to control their transfer chain and stop sever outbreaks in early stages of their appearance.

216 ir cells and the auditory nerve, effectively severing part of the connection between the ear and the

217 We mimicked an economic 'crash', manually severing part of the fungal network (Rhizophagus irregul

218 the filament and revealed different filament severing pattern of stationary and HMM propelled filamen

219 After surgical repair of traumatically severed peripheral nerves, associated muscles are paraly

220 ht on the early immune response processes in severed peripheral nerves, we performed genome-wide tran

221 ll-free system to show that ANKZF1 and Vms1p sever polypeptidyl-tRNAs on RQC complexes by precisely c

222 ond lasers simultaneously, we could scan and sever posterior lateral microtubule neurons [posterior l

223 nalyzed at single-event resolution, the tube-severing process displayed long-lived, highly constricte

224 ses of fluorescently labeled AIP1 during the severing process of cofilin-decorated actin filaments.

225 in filaments facilitates access of the actin-severing protein Adf1 and subsequent filament disassembl

226 By using mutants of the fission yeast actin severing protein Adf1, we observed that contracting AMRs

227 n as SPG4) gene that encodes the microtubule-severing protein called spastin, are the most common cau

228 sin Cdc8, bundling protein fimbrin Fim1, and severing protein coffin Adf1, we examined how their pair

229 defect in mouse mutants that lack the actin-severing protein cofilin 1 (CFL1).

230 e lamellipodium where it activates the actin-severing protein cofilin [6, 7].

231 The mechanistic role of the actin filament severing protein cofilin is now firmly established; howe

232 factor ARHGEF1, MLC20 , MYPT-1 and the actin-severing protein cofilin, but not of RhoA, ROCK2 or c-Sr

233 of Rac1 and its downstream target, the actin-severing protein cofilin, in alcohol consumption prefere

234 star, the orthologue of the vertebrate actin-severing protein Cofilin, to regulate F-actin levels and

235 properties and modulation by the regulatory severing protein cofilin.

236 increased activity of the filamentous actin severing protein cofilin.

237 tigen receptor (BCR) by activating the actin-severing protein cofilin.

238 RPGR interacts with and activates the actin-severing protein gelsolin, and that gelsolin regulates a

239 ion-regulating proteins, including the actin-severing protein gelsolin, to disrupt actin filaments an

240 toplasmic gelsolin (cGSN), an abundant actin-severing protein involved in the depolymerization of act

241 identify a critical role for the microtubule-severing protein katanin p60 in regulating neuronal prog

242 Increased activity of the microtubule severing protein katanin scales the X. tropicalis spindl

243 Cofilin is an actin filament severing protein necessary for fast actin turnover dynam

244 s in the SPAST gene encoding the microtubule-severing protein spastin account for most HSP cases.

245 bule array by overexpressing the microtubule-severing protein Spastin or by inhibiting the C. elegans

246 he SPAST gene, which encodes the microtubule-severing protein spastin, are the most common cause of h

247 two isoforms (M1 and M87) of the microtubule-severing protein spastin, is the chief gene mutated in h

248 Fidgetin is a microtubule-severing protein that pares back the labile domains of m

249 suppresses expression of gelsolin, an actin-severing protein, and rescues spine deficits found in Ts

250 The severing protein, cofilin, renders filaments more compli

251 tin dynamics in photoreceptors via the actin-severing protein, gelsolin.

252 l importance of cofilin, a filamentous actin-severing protein, in actin dynamics and pathogen-trigger

253 reduce the levels of fidgetin, a microtubule-severing protein.

254 uced by muscle contractions relayed by actin-severing proteins and from formin homology 2 domain-cont

255 Actin-severing proteins such as cofilin and contractile myosin

256 ontrol, based on depolymerizing kinesins and severing proteins, have been studied extensively, positi

257 the ADF/cofilin-induced torque increases the severing rate constant 100-fold.

258 ited at bundled microtubule segments and the severing rate of nonbundled microtubules is reduced by M

259 roteins cofilin, profilin, and formin, which sever, recycle, and assemble filaments, respectively.

260 Microtubule severing regulates cytoskeletal rearrangement underlying

261 e filaments, promote branching, and increase severing requires the internal (I/L)WEQ actin-binding do

262 optic nerve crush injury, lengthy growth of severed retinal ganglion cell (RGC) axons occurs only in

263 Axonal regeneration from the severed roots into the SC could be seen by biotinylated

264 lial cells in zebrafish form a bridge across severed spinal cord tissue and facilitate regeneration.

265 c environment, the capacity to reconnect the severed spinal cord via neural stem cell grafts, and mod

266 re silenced or when other sensory organs are severed, suggesting that increased sleep after injury is

267 led scrunching and thereby storing energy to sever the bonds that hold the enzyme at the promoter.

268 o core cognitive capacities even before they sever the link between nonhuman primate vocalizations an

269 y represent promising therapeutic targets to sever the link between obesity and T2DM.

270 s the forces that extract tubulin dimers and sever the microtubule.

271 ting, whereas ESCRT-III and Vps4 function to sever the neck of the forming ILVs.

272 e have altered backbone dynamics, completely severing the allosteric signal yet remarkably, generatin

273 n endocytosis at two different levels by (1) severing the bonds between the PM adaptor proteins Sla2

274 eled aminoacyl disrupts tRNA-T-box stacking, severing the central spine and blocking gene expression.

275 with Cuscuta australis and with and without severing the connection (allowing or preventing integrat

276 le to be extracted from udon or bread witout severing the disulfide bonds under reducing condition.

277 Severing the kinase module from Mediator by removing the

278 However, severing the petiole and applying high-pressure gas coul

279 This approach severs the link between the resonance bandwidth and the

280 Autocatalysis severs the protein into a large membrane-anchored beta s

281 Cs) following optic nerve crush (ONC), which severs their axons and leads to death of ~80% of RGCs wi

282 is phenotype by showing that, in addition to severing, these enzymes modulate microtubule dynamics by

283 lessly transmitted to a smart-phone or cloud sever through the Wi-Fi connection for visualizing the t

284 only with other cooperative individuals and severing ties with everyone else.

285 ods and subject matter in academic work, and severing ties with it may be impossible.

286 Using our system, we specifically sever titin by digestion with TEV protease, and find tha

287 of RNA from the nucleus to the cytoplasm is severed under stress.

288 Here, we investigate the activity of katanin severing using a GFP-labeled human version.

289 New growth at barbed ends generated by severing was blocked specifically in the presence of all

290 e understand key attributes of INF2-mediated severing, we do not understand the mechanism by which IN

291 AIP1 binding and severing were detected preferentially at the boundary be

292 s and enhances actin filament disassembly by severing, which is modulated by tropomyosin.

293 ification rate is predicted to increase with severing, which is, to our knowledge, a new result.

294 n is mediated by branch-specific microtubule severing, which results in local disassembly of the micr

295 ensions, which predicts the importance of MT severing, which we confirm experimentally.

296 We find that local curvature favors severing, while tension surprisingly has no effect on co

297 is predicted to enhance cofilactin filament severing with minimal effects on cofilin occupancy, wher

298 ycolysis and the TCA cycle can be completely severed without affecting normal or neoplastic prolifera

299 Severing would reduce average microtubule length and lif

300 Live calcium imaging in severed zebrafish neurons and temporally controlled phar