ライフサイエンスコーパス: shRNA

1 shRNA (short hairpin RNA)-mediated knockdown of talin1 e

2 shRNA HK2 suppression in HK1(-)HK2(+) liver cancer cells

3 shRNA knockdown of XPB confirmed XPB degradation as the

4 shRNA or CRISPR/Cas9-mediated conditional depletion of T

5 shRNA or small-molecule inhibition of PU.1 in AML cells

6 shRNA-mediated knockdown (KD) of Rab27b increased alphas

7 shRNA-mediated knockdown of isoform 2 in BRAFi resistant

8 shRNA-mediated knockdown of Lmo4 in the nucleus accumben

9 shRNA-mediated knockdown of OLIG2 largely reverses abnor

10 shRNA-mediated Mtss1L knockdown in vivo prevented the ex

11 2 silencing, we screened a library of 60,000 shRNAs using a cell line with a MeCP2 reporter on the Xi

12 h TCM from PC-3 cells transfected with GPC-1 shRNA increased the expression of migration markers, end

13 lung cancer cells using CRISPR/Cas9 or Mcl-1 shRNA significantly decreased Akt activity, leading to s

14 LINE-1 shRNAs can abrogate the HUSH-dependent response, while o

15 t model, TICs stably transduced with LIMK1/2 shRNA were implanted intracranially in immunocompromised

16 infusion or GSK-3beta knockdown by GSK-3beta shRNA in the LA attenuated ZIP-induced disruption of lea

17 Using Thbs4(-/-) mice and TSP-4 shRNA, we found that TSP-4 mediated pro-angiogenic funct

18 By testing 4666 shRNAs derived from the CD95 and CD95L mRNA sequences an

19 artially rescued viral replication in Huh7.5-shRNA CypA cells.

20 In DRG neurons, Zdhhc5/8 shRNA knockdown reduced Gp130 palmitoylation and even mo

21 Using a shRNA library targeting 15,000 genes, a functional genom

22 Neurons electroporated with a shRNA targeting IGF-1 receptor failed to migrate to the

23 white adipose tissues, and heart with an AAV-shRNA (Grb14-shRNA) improves glucose homeostasis in diet

24 ociated adult rat DRGs transduced using AAV5-shRNA-fidgetin on a laminin substrate with spots of aggr

25 Accordingly, shRNA-mediated suppression of Atg5, an E3 ubiquitin liga

26 s shuttle vector to deliver microRNA-adapted shRNA via intracranial injection.

27 primary human triple-mutated AML cells after shRNA-mediated HLF knockdown revealed the NOTCH target H

28 all molecules mAR-SLC39A9-shRNA or G(alphai)-shRNA, and not the classic antiandrogens including enzal

29 e performed sequencing-based screening of an shRNA library on a panel of cancer cells of different or

30 target p21RAS directly, here we performed an shRNA library screen of all human kinases and Rho-GTPase

31 target p21RAS directly, here we performed an shRNA library screen of all human kinases and Rho-GTPase

32 stance in colorectal cancer, we performed an shRNA-based loss-of-function genetic screen using a kino

33 Through an shRNA screen, we identified the protein arginine methylt

34 In this report, we used an shRNA-depletion strategy to overcome the oogenesis block

35 Using an shRNA library targeting enzymes in the ubiquitin proteas

36 ong with CRISPR-mediated gene activation and shRNA knockdown of DYRK1A, we show here that chemical in

37 Chromatin analysis and shRNA-mediated gene suppression experiments indicated a

38 AMPKalpha agonist, antagonist, and shRNA were used to explore the downstream targets of Rsp

39 ells attenuated TRAIL-induced apoptosis, and shRNA-mediated HOTAIR knockdown in TRAIL-resistant PANC-

40 ith enhanced proliferation in the brain, and shRNA-mediated suppression of HIF1A or drug inhibition o

41 RP downregulation induced by CRISPR/Cas9 and shRNA techniques resulted in perturbed axonal pathfindin

42 Through CRISPR and shRNA screening, we identified the histone chaperone CAF

43 Neonatal electroporation and shRNA mediated knockdown of Rab27a in dorsal subventricu

44 factor beta (PDGF-B), constitutive HRAS, and shRNA-p53 respectively.

45 anistically, MST1 interacted with IRAK1, and shRNA-mediated knockdown was sufficient to increase IRAK

46 Mechanistically, OGG1i and shRNA depletion cause S-phase DNA damage, replication st

47 city assays using PrimPol overexpression and shRNA knockdown strains in renal proximal tubular epithe

48 anel, shRNA inactivation of NMD pathway, and shRNA-depletion of RBPs followed by RNA-seq, to identify

49 Through transcriptomic and shRNA knockdown studies, together with analysis of clini

50 Over 80% of multiple-tested siRNAs and shRNAs targeting CD95 or CD95 ligand (CD95L) induce a fo

51 and suppressing the AR with either Enz or AR-shRNA could further increase the olaparib sensitivity to

52 Using FACS-assisted shRNA screens, we identified the cell-surface adhesion r

53 oach for the analysis of proliferation-based shRNA selection strategies and identifies new targets fo

54 Co-administration of TGF-beta shRNA and HBV dual-shRNA decreased HBV DNA, HBV RNA, HBs

55 Silencing NuMA1 or protein 4.1B by shRNA disrupts AIS assembly, but not maintenance.

56 Stable knockdown of ACTN4 by shRNA in HPCs significantly reduces dexamethasone-mediat

57 Knockdown of AhR by shRNA decreased BCRP expression, and this decrease was r

58 ndogenous ADAM15 expression in T47D cells by shRNA reduced endogenous Claudin-1 expression confirming

59 BACH1 depletion by shRNA or degradation by hemin sensitizes cells to ETC in

60 GLUL downregulation by shRNA caused a 40% increase in the methylglyoxal level,

61 ed by overexpression of DUOX1 or enhanced by shRNA-dependent DUOX1 silencing.

62 Inhibition of NRP1 expression by shRNA in both pancreatic and lung cancer cells containin

63 Suppression of PGC-1alpha expression by shRNA in the PGC-1alpha-positive U343MG glioblastoma lin

64 Inhibition of GAP-43 expression by shRNA significantly reduced seizure duration and severit

65 systems using isoform-specific knockdown by shRNA and isoform-specific knockout by CRISPR/Cas9.

66 Here, Abi1 knockdown by shRNA reduced human airway smooth muscle cell migration,

67 Silencing EGFL6 mRNA by shRNA transfection of cancer cells also significantly re

68 letion of JB12 during reductive stress or by shRNA from Huh-7 cells was associated with accumulation

69 inase, with a specific inhibitor TMI-1 or by shRNA knockdown prevented ovarian cancer cells from rele

70 Down-regulation of PKM2 by shRNA blunts cellular responses to shikonin but enhances

71 Targeted depletion of PPM1A by shRNA or inhibition of PPM1A activity by sanguinarine re

72 ional assay revealed that targeting PRKDC by shRNA and NU7026 (specific PRKDC inhibitor) could enhanc

73 rthermore, when dorsal CA1 Ih was reduced by shRNA-HCN1, the CUS-induced behavioral deficits were pre

74 hibitory role for TRAIL-R3/4 was revealed by shRNA knockdown and mAb blockade, showing that these reg

75 eover, blocking the PTN-PTPRZ1 signalling by shRNA or anti-PTPRZ1 antibody potently suppressed GBM tu

76 nes that promote cell death when silenced by shRNA in the presence of G4-stabilising small molecules.

77 I (NDUFV1) and complex II (SDHC) subunits by shRNA in B16rho(0) cells abolished or significantly reta

78 5, whereas ERBB3 dependency was validated by shRNA-mediated silencing.

79 Suppression of TopBP1 by shRNAs impairs HPV genome amplification and activation o

80 Vector carrying shRNA against TGF-beta, though did not inhibit HBV repli

81 Genetic (CD44v6 shRNA) or a small molecule inhibitor (CD44v6 peptide) ta

82 Viral delivery of circ_Lrp6 shRNA prevented intimal hyperplasia in mouse carotids.

83 In contrast, shRNA-mediated targeting of BST2 demonstrated that BST2

84 nd we identified this induction as critical; shRNA-mediated downregulation of p57Kip2, but not the re

85 aightforward, and scalable method to deliver shRNAs into fertilized eggs of the hydrozoan cnidarian H

86 Gene silencing with virally delivered shRNA represents a promising approach for treatment of i

87 Interestingly, two different shRNA screens failed to identify a single TP53 target ge

88 ginine-creatine metabolism by CKMT1-directed shRNAs or by the small molecule cyclocreatine selectivel

89 tions, the robust expression of U6/H1-driven shRNAs can induce toxicity and generate heterogeneous sm

90 dministration of TGF-beta shRNA and HBV dual-shRNA decreased HBV DNA, HBV RNA, HBsAg, HBeAg, and live

91 hms and reliably predicts the most efficient shRNAs for a given gene.

92 Lowering PNPLA3 levels by either shRNA knockdown or proteolysis-targeting chimera (PROTAC

93 contrast, silencing LNK expression by either shRNA or CRISPR-Cas9 potentiates the killing effect of I

94 Silencing of XPO1 by either shRNA or selinexor significantly reduced cellular growth

95 PRMT5 inhibition with either shRNA-mediated knockdown or a specific small molecule PR

96 independent screens, BioID and an Epigenome shRNA dropout screen, to define ZEB1 interactors that ar

97 sing a two-step, in vitro/in vivo epigenomic shRNA inhibition screen, we determine the chromatin remo

98 ected with adeno-associated virus expressing shRNA against Cldn5 caused infiltration of the periphera

99 ed that patient-derived GBM cells expressing shRNAs of VEGF or neuropilin-1 (NRP-1) attenuate cancer

100 efore, in this study, we conducted a focused shRNA screen of chromatin modifying enzymes previously s

101 A reductase-focused shRNA screen in UT-SCC-74B cells similarly identified mi

102 system reduces the coding space required for shRNA expression by >2-fold as compared to the typical U

103 These data present a novel strategy for shRNA delivery using CD30 RNA aptamers to down-regulate

104 AB system outperformed a combination of four shRNAs targeting the PTEN transcript, a construct previo

105 Furthermore, shRNA COASY knockdown disrupted downstream PI3K pathway

106 We identify PRMT5 in an in vivo GBM shRNA screen and show that PRMT5 knockdown or inhibition

107 particularly when delivery of multiple gene/shRNA combinations is required.

108 ncrease of PGC1alpha in response to genetic (shRNA and CRISPR/Cas9) and pharmacologic (crizotinib) in

109 rted, RNAi-mediated silencing in a HepG2/GFP-shRNA RNAi sensor line.

110 AAVs coding for glutaredoxin-1 (Glrx) shRNA successfully inhibited Glrx expression by ~66% in

111 DZ dominant-negative mutant or possibly GODZ shRNA, should be considered a potential alternative ther

112 tissues, and heart with an AAV-shRNA (Grb14-shRNA) improves glucose homeostasis in diet-induced obes

113 alginate bead model, induced short hairpin (shRNA) knockdown or overexpression of MCT1 quantitativel

114 tifibrotic activities of single and dual HBV shRNAs.

115 stabilization accelerated, whereas HIF1alpha shRNA delayed wound closure.

116 However, studies using HK2 shRNA and isogenic HK1(+)HK2(-) and HK1(+)HK2(+) tumor c

117 orylation and fatty acid oxidation using HK2 shRNA and small-molecule drugs, prevented human liver HK

118 s are sensitive to cytostasis induced by HK2(shRNA) knockdown and are also sensitive to synthetic let

119 hality in response to the combination of HK2(shRNA) knockdown, an oxidative phosphorylation (OXPHOS)

120 he BLA with stereotaxic injection of 5-HT2CR shRNA AAV vector decreased vocalizations and anxiety- an

121 Finally, targeted IL8 shRNA inhibited BM-MSC-induced AML survival.

122 ngle amino acid mutations result in impaired shRNA-mediated silencing.

123 Importantly, shRNA-mediated NKX1-2 knockdown in 3T3-L1 preadipocytes

124 and restored reduced filopodia formation in shRNA-induced DAAM2-knockdown podocytes.

125 RAGE knockdown with multiple independent shRNAs in breast cancer cells led to decreased transwell

126 dogenous Aalpha was targeted by a co-induced shRNA, endogenous B subunits were rapidly degraded, resu

127 val of murine PDAC cells, using an inducible shRNA-based system that enables temporal control of Kras

128 hich eEF2K had been depleted by an inducible shRNA.

129 invasion by using the doxycycline-inducible shRNA system.

130 Finally, combining AAV-ISL2-shRNA with temozolomide suppressed oligodendroglioma pro

131 onstrated that the deficiency of ATG7 by its shRNA dramatically reduced sphere formation and invasion

132 Furthermore, a synthetic lethal kinome shRNA screen with a pan-ERBB inhibitor, AZD8931, identif

133 We further show using KLF2 shRNA that the inhibitory effect of NMP on endothelial i

134 Lentiviral shRNA knockdown and reconstitution experiments revealed

135 Lentiviral shRNA-mediated knockdown (KD) of PKCiota leads to decrea

136 a-catenin activity in vivo, where lentiviral shRNA depletion of Ror2 expression augmented canonical W

137 e performed a genome-scale pooled lentiviral-shRNA library screen in cells that represent nonmetastat

138 of corrected channel function, or high-level shRNA knockdown of CFTR or F508del-CFTR.

139 Using lentiviral-mediated shRNA knockdown in rat hippocampal neurons, we find that

140 Xenografts expressing MIF-shRNA grew more rapidly with greater angiogenesis and ha

141 Moreover, shRNA-induced Gadd45b knockdown decreases expression of

142 Moreover, shRNA-mediated knockdown of CD38 inhibits mitochondrial

143 he interpretation of the effects of multiple shRNAs over multiple cell line passages.

144 nes located under the GWAS peak via multiple shRNAs, only TMEM175 was found to consistently influence

145 Transducing 3D CPCs with Notch1-shRNA (short hairpin RNA) did not reduce retention, but

146 We found that NRP1 shRNA expressing KRAS (mt) tumor cells caused increased

147 is step enables high-throughput screening of shRNA or CRISPR-Cas9 constructs to identify genes that r

148 Our results suggest that the combination of shRNAs against HBV and TGF-beta could be developed into

149 Lentiviral-mediated delivery of shRNAs was used to modulate expression of 66 genes in as

150 Our detailed protocol for electroporation of shRNAs in H. symbiolongicarpus embryos constitutes an im

151 Here, we used in utero electroporation of shRNAs or LIC functional domains to determine the relati

152 Mice with inducible expression of shRNAs against Anln mRNA developed fewer liver tumors af

153 tion protocol allows for the transfection of shRNAs into hundreds of fertilized H. symbiolongicarpus

154 n inhibitors Iminodyn-22 and Dynole-34-2, or shRNA-mediated downregulation of DNM2, impaired NME's ab

155 Antibody- or shRNA-mediated functional ABCB5 blockade inhibited proli

156 primary fibroblast cells via CRISPR/Cas9 or shRNA recapitulated mTert (-/-) phenotypes of accelerate

157 sion of dominant-negative VAV3 constructs or shRNA-mediated down-regulation of VAV3 expression in bre

158 we showed that although genetic deletion or shRNA-mediated suppression of IGF2BP1 did not affect pri

159 Conversely, genetic or shRNA-mediated conditional KO/knockdown of GSK3beta redu

160 F138 function with a mutant (RNF138-H36E) or shRNA infection significantly upregulates the CaV2.1 pro

161 Pharmacologic inhibition or shRNA knockdown of ILK prevented periostin-induced Akt/m

162 lization in the presence of CK2 inhibitor or shRNA targeting CK2alpha.

163 blocked using pharmacological inhibitors or shRNA knock-down of BK channels.

164 are selective class IIa HDAC inhibitors, or shRNA-mediated knockdown of HDAC5 but not HDAC9 leads to

165 thesis, antagonism of AT1R using losartan or shRNA-mediated knockdown in melanoma cell lines expressi

166 nd inhibition of 5-Lox by Quiflapon/MK591 or shRNA interrupts oncogenic c-Myc signaling and kills ERP

167 Pharmacological or shRNA-mediated inhibition of PLK4, or mutation of the NE

168 on of a GDP-bound Rab5 mutant (Rab5/S34N) or shRNA-mediated knockdown of endogenous Rab5 prevented FA

169 MRTF-A knockdown by siRNA or shRNA impaired TGF-beta1 and TCM induction of alpha-SMA

170 Using siRNA- or shRNA-mediated knockdown, we demonstrate that, in contra

171 Our shRNA/rescue studies revealed that Shank3 binding to bot

172 sources, eCLIP assays for a large RBP panel, shRNA inactivation of NMD pathway, and shRNA-depletion o

173 latin increased protein PARylation and PARP1 shRNA knock-down returned PRPP towards normal, and (2) d

174 n the lentiviral vector that carries a Prkcd shRNA overcame the adverse effects of Prkcd knockdown on

175 ce were injected with scramble shRNA and PRR shRNA and followed for a period of eight weeks.

176 In vivo, targeting RAGE shRNA knockdown in human and mouse breast cancer cells,

177 RARG shRNA approaches in non-malignant (RWPE-1 and HPr1-AR) a

178 nt mice which express tetracycline-regulated shRNAs broadly targeting the main components of the HIF/

179 Using retroviral shRNA knockdown, we have demonstrated that these JAK inh

180 s with DNA-repair-focused small hairpin RNA (shRNA) and CRISPR (clustered regularly interspaced short

181 ens in the CNS using both short hairpin RNA (shRNA) and CRISPR libraries.

182 However, short hairpin RNA (shRNA) and CRISPR-mediated targeting of ISG15 indicated

183 We show that short hairpin RNA (shRNA) depletion of TET2 results in a decrease in latenc

184 ells, ZIP12 deficiency by short hairpin RNA (shRNA) depletion or CRISPR/Cas9 genome editing resulted

185 l interfering RNA (siRNA)/short hairpin RNA (shRNA) gene knockdown and clustered regularly interspace

186 ositive vesicles based on short hairpin RNA (shRNA) gene silencing and the colocalization of LAMP-1,

187 hile PTEN repression with short hairpin RNA (shRNA) improves regeneration but to a lesser extent, lik

188 t depletion of EYA1 using short hairpin RNA (shRNA) in breast cancer cells destabilizes the Myc prote

189 TIM1 antibodies and STIM1 short hairpin RNA (shRNA) in wild-type VSMCs, and was absent in TRPC1(-/-)

190 5gamma by transfection of short hairpin RNA (shRNA) or in which the gene had been knocked out (DeltaG

191 duced with Trpm4-targeted short hairpin RNA (shRNA) progressively decreased the tidal volume of breat

192 comprehensive genome-wide short hairpin RNA (shRNA) screen identified >350 genes modulating lipotoxic

193 resistance in an in vivo small hairpin RNA (shRNA) screen of 730 genes deleted in prostate cancer.

194 Through a short hairpin RNA (shRNA) screening, we identified single-stranded DNA bind

195 enous NFAT3 expression by short hairpin RNA (shRNA) significantly inhibited tumor cell proliferation,

196 ers and cognate-inducible short hairpin RNA (shRNA) targeted against the reporter coding region, we h

197 l vector, which encodes a short hairpin RNA (shRNA) targeting BCL11A mRNA embedded in a microRNA (shm

198 tiviral vector encoding a short hairpin RNA (shRNA) targeting Grb2 decreased the expression of Grb2 a

199 ial compensation, we used short hairpin RNA (shRNA) to acutely knock down Sgce in the adult mouse and

200 r was utilized to deliver short hairpin RNA (shRNA) to CD30(+) T cells to target retinoic acid recept

201 s (AAV) vector expressing short hairpin RNA (shRNA) to knock down the translation of GLP1R mRNA (GLP1

202 roblasts using lentiviral short hairpin RNA (shRNA) transduction.

203 Using small hairpin RNA (shRNA), which was validated in cultured neuronal cells,

204 We demonstrate that short hairpin RNA (shRNA)-mediated knockdown of Megf10, as well as overexpr

205 Indeed, short-hairpin RNA (shRNA)-mediated knockdown of p62 impaired breast cancer

206 Similarly, small hairpin RNA (shRNA)-mediated reduction of IL6 or LIF in CA-MSC partia

207 significantly reversed by short hairpin RNA (shRNA)-mediated selective depletion of DPYSL2A.

208 69(-/-) reporter mice and short hairpin RNA (shRNA)-mediated silencing and miR-155 transfection appro

209 ls stably transduced with small hairpin RNA (shRNA)-STAT3 into immunodeficient mice revealed a decrea

210 Using short hairpin RNAs (shRNAs) against VASH1, VASH2, and SVBP, we showed that b

211 as confirmed by distinct short hairpin RNAs (shRNAs) and high sensitivity of the cell lines to AZD805

212 Short hairpin RNAs (shRNAs) are effective in generating stable repression of

213 B, DDB1 or DCAF11, using short hairpin RNAs (shRNAs) attenuated the ubiquitination level of p21(Cip1)

214 studies have shown that short hairpin RNAs (shRNAs) can induce gene-specific knockdowns in two cnida

215 as engineered to produce short hairpin RNAs (shRNAs) corresponding to the Aedes aegypti orthologs of

216 , Aurora B knock down by short-hairpin RNAs (shRNAs) suppresses AKT/GSK3beta/Snail1 signaling, revers

217 nsequences of expressing short hairpin RNAs (shRNAs).

218 ct potent microRNA-based short hairpin RNAs (shRNAs).

219 on was knocked down with short hairpin RNAs (shRNAs).

220 ate consisting of CD30 aptamer and RORgammat shRNA sequences was synthesized and was transcribed CD30

221 d and was transcribed CD30 aptamer-RORgammat shRNA chimera (CD30-AshR-RORgammat).

222 The RORgammat shRNA moiety of CD30-AshR-RORgammat chimera was cleaved

223 tic C57BL/6 mice were injected with scramble shRNA and PRR shRNA and followed for a period of eight w

224 n the zebrafish eye as compared to scrambled shRNA control (p = 0.0005).

225 pes of ovarian cancer cells expressing SHMT1 shRNAs.

226 We now show these si/shRNAs kill cancer cells through canonical RNAi by targe

227 , and used molecular genetics methods (siRNA/shRNA gene knockdown and CRISPR-mediated transcriptional

228 2 cell-derived xenografts expressing SLC13A5-shRNA in nude mice.

229 9 signaling with small molecules mAR-SLC39A9-shRNA or G(alphai)-shRNA, and not the classic antiandrog

230 Indeed, astrocyte-specific shRNA- and CRISPR/Cas9-driven gene inactivation combined

231 ntibody or deletion of ApoE via its specific shRNA.

232 tions of activating TopBP1 protein, specific shRNAs, or chemical inhibitors for ATR, ATM, and/or DNA-

233 We have discovered using stable shRNA gene suppression that GnT-III expression controls

234 sensor GFP-PLCdelta1-PH was reduced by STIM1 shRNA and absent in TRPC1(-/-) cells.

235 Knockout of NF-kappaB subunits, shRNA-mediated knockdown of GATA-6, or pharmacologic inh

236 so provide visual confirmation of successful shRNA transfection inside embryos through electroporatio

237 RISPR/Cas9 or stably-expressed TDP2-targeted shRNA and transfection of various TDP2 mutants to show t

238 Mice transduced with Trpc3-targeted shRNA survived with no changes in breathing.

239 on mitochondrial phenotype, we utilized Taz-shRNA knockdown (Taz(KD) ) mice, which exhibit defective

240 SPR system also worked more effectively than shRNAs for Pten repression in rat neural crest-derived P

241 te1 can result from chronic stress, and that shRNA-reduced Ate1 increases cellular resistance to stre

242 Importantly, we demonstrated that shRNA-mediated downregulation of Mitf expression or inhi

243 l culture models of human OCCC, we find that shRNA silencing of SPINK1 sensitizes tumor cells to anoi

244 Finally, we found that shRNA knockdown of STAG2 and SMC1A causes aberrant expre

245 rroborating these findings, we observed that shRNA-mediated knockdown of RAC1 reduces cell proliferat

246 rroborating these findings, we observed that shRNA-mediated knockdown of RAC1 reduces cell proliferat

247 Here we show that shRNA-mediated depletion of the m(6)A-forming enzyme MET

248 The shRNA library was designed to target a subset of genes p

249 The shRNA-mediated knockdown of Rbcn3beta/WDR7 redistributed

250 More importantly, the shRNA-mediated knockdown of atypical PKCtau reversed NT-

251 gle-cell RNA sequencing (scRNA-seq) with the shRNA screen to investigate the mechanism of action of t

252 Finally, cells electroporated with the shRNA targeting IGF-1 receptor were unable to form an ax

253 This was validated through shRNA-mediated knockdown of the target protein, HNF1beta

254 Ahigh-throughput shRNA library screen in IRF8 expression-restrictive cell

255 eloid leukemia cell line K562 in response to shRNA knockdown of the RNA editing enzyme ADAR1.

256 global gene expression signature similar to shRNA depletion of MSI2.

257 n be visualized in single cells subjected to shRNA knockdown or CRISPR-Cas9 gene editing.

258 ules, including tretinoin, metformin, or TR4-shRNAs, all led to increase the sunitinib sensitivity to

259 med MDA(w)-tumors with a lentiviral-TRAF3IP2-shRNA not only regressed their size, but also prevented

260 ective effects were observed after transient shRNA-mediated silencing of Rps19, but not several other

261 overexpression or silencing (piLenti-TWIST1-shRNA-GFP), respectively, further confirmed improved neo

262 In this study, we employed an unbiased shRNA screening coupled with ultra-fast sequencing to sc

263 t NOX1 plays in colon cancer growth, we used shRNA to decrease NOX1 expression stably in HT-29 human

264 Using shRNA knockdown, we confirmed c-Myc regulation of expres

265 Using shRNA knockdown, we validate KDM8's functions as a regul

266 Using shRNA to knockdown Diaph1 or SMIFH2 to target Diaph1 act

267 Using shRNA, we established cultured mouse podocytes with beta

268 Knocking down Cav-1 using shRNA in HEK cells expressing the familial AD-linked APP

269 tagonists or genetic targeting of A2AR using shRNA.

270 te of wound healing while its ablation using shRNA reduced healing compared to matched controls.

271 down of ACVR1C in injected tumor cells using shRNA also resulted in a 54% reduction in tumor dissemin

272 1-loss in patient-derived glioma cells using shRNA increases self-renewal, heightens cell invasion, a

273 nerated in SUM149 and BT549 TNBC cells using shRNA lentiviral vectors.

274 ctive reduction of Ephexin5 expression using shRNA in the dentate gyrus of presymptomatic adolescent

275 d to telomeres, while SIRT6 inhibition using shRNA or a deacetylase-inactive mutant (SIRT6(H133Y)) sh

276 genetic downregulation of the receptor using shRNA potently suppressed invasion, growth, survival, an

277 hetic lethal genes is tested in silico using shRNA and drug screening data from cancer cell line data

278 In this study, using shRNA- and CRISPR/Cas9-mediated gene silencing and knock

279 USP13 shRNA knockdown increases alpha-synuclein ubiquitination

280 TRX1 inhibition via shRNA or a phase I-approved inhibitor, PX-12 (untested i

281 In vivo TRX1 inhibition via shRNA or PX-12 reverses the castration-resistant phenoty

282 Conversely, acute suppression of RNF145 via shRNA-mediated knockdown, or chronic inactivation of RNF

283 his question, we used a combination of viral shRNA and conditional mutation to produce cell-specific

284 ubiquitylate the water channel AQP2 in vitro shRNA knockdown of CHIP in CCD cells increased AQP2 prot

285 of the ABO gene was assessed using in vitro shRNA-mediated knockdown of gene expression in the murin

286 In vivo shRNA knockdown of Ptprb in the cleared mammary fat pad

287 shment of the hypoxia-sensing machinery when shRNA expression was discontinued.

288 Using a combined pooled-genome wide shRNA library screen and global proteomic profiling, we

289 A genome-wide shRNA screen identified the transcriptional coactivators

290 ss in mammalian cells, we used a genome-wide shRNA screen in K562 cancer cells to identify genes that

291 duced senescence, we performed a genome-wide shRNA screen in primary human fibroblasts expressing OSK

292 Here, in a genome-wide shRNA-based screen, we identified nuclear export factor

293 H2.35 liver cells with shRNA knockdown of ANLN formed tumors more slowly in FRG

294 e specifically target the GSK3 isoforms with shRNA knock-down in mouse hippocampus and with novel iso

295 Targeting ITPKB with shRNA or its small-molecule inhibitor resulted in attenu

296 ide lipase (ATGL) in human pseudoislets with shRNA targeting ATGL (shATGL) increased triglycerides (T

297 Knockdown of AR with shRNAs and a new generation anti-androgen drug, Enzaluta

298 iched for PCFUs, and virally transduced with shRNAs to knock down GLIS3 and other proteins.

299 in K14-Cre;Wnt10a(flox/flox) molars by Wnt4 shRNA adenovirus and kidney capsule grafts, the root fur

300 stent with a cell-autonomous role of Zfp423, shRNA-mediated knockdown of Zfp423 in myoblasts inhibits