ライフサイエンスコーパス: shuttle vector

1 t2 gene in an Escherichia coli/S. pneumoniae shuttle vector.

2 he M. smegmatis chromosome, with loss of the shuttle vector.

3 corresponding wild-type allele in trans on a shuttle vector.

4 gene is expressed in trans from a borrelial shuttle vector.

5 were constructed in a temperature-sensitive shuttle vector.

6 g a modification of a highly versatile yeast shuttle vector.

7 ve promoter on an Escherichia coli-S. aureus shuttle vector.

8 artificial chromosome or in a yeast-E. coli shuttle vector.

9 and then delivered into P. haemolytica on a shuttle vector.

10 2, an Escherichia coli-yeast low-copy-number shuttle vector.

11 d into an Escherichia coli-C. acetobutylicum shuttle vector.

12 6 and from an ospC promoter-lacZ fusion on a shuttle vector.

13 by introducing a wild-type copy of irvR on a shuttle vector.

14 r expansions of CAG*CTG repeats present on a shuttle vector.

15 lls, a new genetic assay was created using a shuttle vector.

16 e if the BBE22 gene on lp25 is provided on a shuttle vector.

17 site-specific recombination into a bacterial shuttle vector.

18 plicating Methanosarcina-Escherichia plasmid shuttle vectors.

19 easible to study these events using episomal shuttle vectors.

20 ed the frequency of DNA repair of UV-damaged shuttle vectors.

21 estigations in E. coli using single-stranded shuttle vectors.

22 The GFP shuttle vector also encoded ampicillin resistance and co

23 ietic cells (n = 1,821) by using a bacterial shuttle vector and a comparable analysis of lentiviral v

24 re, and most importantly, by using a peptide shuttle vector and four independent antigens, we demonst

25 G2, were incorporated into a single-stranded shuttle vector and introduced into Escherichia coli or s

26 rmosensitive, Escherichia coli-mycobacterial shuttle vector and introduced into M. smegmatis mc2155.

27 loned into a Methanosarcina-Escherichia coli shuttle vector and mutagenized with hydroxylamine.

28 ecifically into a SV40/BK virus origin-based shuttle vector and replicated in xeroderma pigmentosum c

29 in three CpG methylated target genes: a supF shuttle vector and the cII and lacI transgenes in embryo

30 DPP1(H223A) alleles were cloned into a yeast shuttle vector and then expressed in a dpp1Delta lpp1Del

31 within the supF reporter gene in an episomal shuttle vector and to direct site-specific photoadduct f

32 Here, we construct shuttle vectors and develop methods to transform E. lent

33 DNA transformation methods, reporter genes, shuttle vectors and expression vectors.

34 Plasmid shuttle vectors and insertion cassettes that encode PA(r

35 -BPDE were incorporated into single-stranded shuttle vectors and transfected into simian kidney cells

36 HeLa cells were transduced with an AAV shuttle vector, and integrated proviruses containing fla

37 inal repeat (LTR) of a spleen necrosis virus shuttle vector, and proviruses were recovered from infec

38 In the shuttle vector approach, exogenous gene products that en

39 Replicative errors in this shuttle vector are detected as mutations in a marker gen

40 SV40-based shuttle vectors are popular because of their ease of use

41 These shuttle vectors are useful for genetic analyses, as well

42 Shuttle vectors are useful tools for studying DNA replic

43 e supF gene is not in the same region of the shuttle vector as the T-antigen gene it appears that sec

44 mation for further use of chlamydial plasmid shuttle vectors as genetic tools to understand chlamydia

45 reased the ease with which one can clone the shuttle vectors, as well as screen for co-integrated and

46 Using a shuttle vector assay, we measured mutation rates at repo

47 a mammalian system was tested by SV40-based shuttle vector assay.

48 After methylation of the shuttle vector at all CpG sequences, 42% of all G-to-T t

49 s PPT from RSVP(A)Z, a replication-competent shuttle vector based on Rous sarcoma virus (RSV), with a

50 f a new Escherichia coli-Treponema denticola shuttle vector based on the naturally occurring spiroche

51 An expression shuttle vector, based on the cryptic plasmid pURB500 and

52 y insertion/deletion mutations detected by a shuttle vector-based assay to a greater extent than loss

53 All previously reported shuttle vector-based methods for investigating the cytot

54 ding 4-fold higher mutation frequencies in a shuttle vector-based mutagenesis assay designed to detec

55 - to 5-fold higher mutation frequencies in a shuttle vector-based mutagenesis assay designed to detec

56 and incorporating it with a widely used supF shuttle vector-based mutagenesis system, we can convenie

57 In this study, using a shuttle vector-based strand-specific PCR-competitive rep

58 hromosome (PAC) clones into a yeast-bacteria shuttle vector by using recombinogenic targeting.

59 While transformation with a shuttle vector carrying ospC under the control of a cons

60 er-defective pRS01 derivative (pM1014) and a shuttle vector carrying the ltrB region, including the L

61 Here, we constructed single-stranded M13 shuttle vectors carrying a (S)G, S(6)mG, or (SO3H)G at a

62 r beta-glucuronidase; E. coli-C. perfringens shuttle vectors carrying the fusions were introduced int

63 Furthermore, we simplify the shuttle vector cloning to one step by incorporation of a

64 ed using pHP1, an Escherichia coli-H. pylori shuttle vector conferring kanamycin resistance.

65 A single-stranded shuttle vector containing 5'TCCTCCTCXCCTCTC (X = dG-AAF,

66 A shuttle vector containing a regulated muscle-specific pr

67 nsformation of a spaP-negative mutant with a shuttle vector containing an internally deleted spaP lac

68 We are currently constructing a shuttle vector containing both the wild-type gapA and cr

69 lia burgdorferi, that was transformed with a shuttle vector containing glpTQ from B. hermsii produced

70 te upstream of the bb0729 coding sequence; a shuttle vector containing the bb0729-cdr operon and upst

71 A shuttle vector containing the CA-11.2A cp32 plasmid main

72 es, gonococci were transformed with a hybrid shuttle vector containing the gfp gene from Aequoria vic

73 The second assay used an SNV-based shuttle vector containing the lacZ alpha gene.

74 s in cells, we constructed a single-stranded shuttle vector containing the lesion in 5'-TGT and 5'-TG

75 mation of B. burgdorferi lacking lp25 with a shuttle vector containing the lp25 gene BBE22 (pBBE22) r

76 Single-stranded shuttle vectors containing (5)(')TCCTCCTCXCCTCTC (X = dG

77 al mutagenesis was significantly higher when shuttle vectors containing dA opposite one of the lesion

78 human cells, replication of single-stranded shuttle vectors containing Fapy*dG is more mutagenic tha

79 Recombinant shuttle vectors containing individual sar promoters upst

80 When the shuttle vector contains a Fapy*dG:dA base pair, as high

81 The shuttle vector contains a promoter-TNR-reporter gene con

82 the combination of fingerprint analysis of a shuttle vector cosmid library and probe hybridization.

83 oaches were feasible with Chlamydia and that shuttle vectors could be selected and maintained within

84 infectious wild-type clone with incompatible shuttle vectors derived from the native plasmids, render

85 ading frames was used to construct a smaller shuttle vector, designated pBSV2.

86 ecoverable, chromosomally based lambda phage shuttle vector designed to report mutations without the

87 as the yeast selectable marker into a set of shuttle vectors designed to express foreign genes in P.

88 ctivity, while complementation with p66 on a shuttle vector did not restore infectivity.

89 A in human cells, we reacted MDA with pSP189 shuttle vector DNA and then transfected them into human

90 ent B. burgdorferi limit transformation with shuttle vector DNA from E. coli, irrespective of its dam

91 q67, whose presence limits transformation by shuttle vector DNA from Escherichia coli.

92 ane segment and subcloned into an adenoviral shuttle vector downstream of a cytomegalovirus promoter

93 Shuttle-vector experiments showed that ascorbate-Cr-DNA

94 n cheX mutant cells were complemented with a shuttle vector expressing CheX.

95 cDNA was cloned into the pac.Ad5.CMV.K-N.pA shuttle vector for generation of replication-deficient a

96 availability of a new selectable marker and shuttle vector for genetically dissecting B. burgdorferi

97 wild-type transgenic mice carrying a lambda shuttle vector for mutation detection.

98 ggest that E faecalis could act as a natural shuttle vector for the wide dissemination of pX3_NDM-5 p

99 Shuttle vectors for facile gap repair cloning and integr

100 plasmids provided an opportunity to develop shuttle vectors for transformation of rickettsiae.

101 We further constructed single-stranded M13 shuttle vectors harboring individual diastereomers of N(

102 e functional studies, a gene trap retrovirus shuttle vector has been developed to disrupt genes expre

103 del system with an Epstein-Barr virus- based shuttle vector have been characterized.

104 st, several autonomously replicating plasmid shuttle vectors have been constructed based on the natur

105 we show that, when BBK32 was produced from a shuttle vector in an otherwise nonadherent high-passage

106 Bypass of Fapy.dG in a shuttle vector in COS-7 cells produces G --> T transvers

107 of a supF reporter gene contained in a SV40 shuttle vector in mammalian cells.

108 somally integrated, recoverable lambda phage shuttle vector in mouse fibroblasts.

109 ments were carried out using double-stranded shuttle vectors in HeLa cell nuclear lysates and in HEK

110 Each shuttle vector includes an MCS and a selectable antibiot

111 introduction of the wlbpe or wlbbr loci on a shuttle vector into the three delta wlb mutants restored

112 The shuttle vector is propagated in cultured cells, then rec

113 one complication with the use of SV40-based shuttle vectors is the interaction of cellular p53 prote

114 By engineering appropriate recombinant shuttle vectors, it is feasible to examine mutations by

115 Here, we utilized a shuttle vector method to examine the efficiency and fide

116 plication in Escherichia coli by employing a shuttle-vector method.

117 ate mobilization by pAD1 when ligated to the shuttle vector pAM401; however, it was not mobilized by

118 nt which contains all three fsr genes in the shuttle vector, pAT18.

119 The shuttle vector pAvCvSv-hTopIIalpha was constructed and c

120 plementing the pgaABCD locus in trans in the shuttle vector pBAD18kan-ori, plasmid Deltapga-c, restor

121 l regions of infectivity were restored via a shuttle vector pBBE22 with or without an intact copy of

122 The recombinant shuttle vector pBBE22, which includes the virulence dete

123 hat have increased transformability with the shuttle vector pBSV2 were recently constructed by inacti

124 f transformation by electroporation with the shuttle vector pBSV2, an autonomously replicating plasmi

125 e comaintained in B. burgdorferi with extant shuttle vector pCE320, which has a replication origin de

126 ted recombination between the yeast-bacteria shuttle vector, pClasper, and various P1 clones to trans

127 nomic DNA selectively into a yeast-bacterial shuttle vector, pClasper, by recombinogenic targeting in

128 The shuttle vector, pDLT44, for M. maripaludis JJ was constr

129 cloned into the Escherichia coli-Bacteroides shuttle vectors pFD288 and pFD340 and was expressed in B

130 quences inserted using Cre recombinase and a shuttle vector, pFloxin.

131 nonmobilizable Bacteroides-Escherichia coli shuttle vector pGAT400DeltaBglII using a functional assa

132 ragilis LV23 by using the transfer-deficient shuttle vector pGAT400DeltaBglII.

133 nonmobilizable Escherichia coli-Bacteroides shuttle vector pGAT400DeltaBglII.

134 be transformed with chlamydial plasmid-based shuttle vectors pGFP::SW2 and pBRCT.

135 omatis, serovar L2, with either the original shuttle vector (pGFP::SW2) or a derivative of pGFP::SW2

136 ,000-member human genomic library in the PAC shuttle vector pJCPAC-Mam2 that can be propagated in bot

137 e T. denticola flgE gene was cloned into the shuttle vector pKMCou, and the vector was transformed in

138 We have devised a shuttle vector plasmid assay that reports the stability

139 Utilizing the pSP189 shuttle vector plasmid we found that these ternary DNA a

140 ased retroviral shuttle vector, RSVP (RCASBP shuttle vector plasmid), containing either the zeocin or

141 Using a shuttle vector plasmid, pSP189, cell lines from three pa

142 t cells in the supF marker gene carried in a shuttle vector plasmid.

143 eplication yielded very few of these deleted shuttle vector plasmids (15%).

144 The mutation frequency of pS189 shuttle vector plasmids is higher in human oral keratino

145 e and the lst gene were each cloned into the shuttle vector pLS88 after polymerase chain reaction amp

146 serotype 5a strain J45 were cloned into the shuttle vector pLS88 and electroporated into 4074Deltacp

147 H. somnus 129Pt was electrotransformed with shuttle vector pLS88 containing lob-2A, its LOS electrop

148 The gram-positive shuttle vector pMAD was used as the backbone for an inte

149 A shuttle vector, pMAX-121, was generated that contains el

150 n genes were cloned into the nisin-inducible shuttle vector pMSP3545, nisin induction of holin and ly

151 plasmid library was constructed by using the shuttle vector pOLYG.

152 c integration are being investigated using a shuttle vector, propagated as a stable episome in cultur

153 recombination between the original BAC and a shuttle vector providing the mutation.

154 and gadd45, and the mutation frequency of a shuttle vector pS189 in normal human oral keratinocytes,

155 uced by 4-HNE-dG adducts in the supF gene of shuttle vector pSP189 replicated in human cells.

156 The shuttle vector pSP189, containing the supF gene, was tre

157 gene in Lactococcus in the high-copy-number shuttle vector pTRKH2 were unsuccessful.

158 A new Streptomyces-Escherichia coli shuttle vector, pUCS75, has been constructed to permit f

159 In contrast to other commonly used shuttle vectors, pUCS75 retains the primary site for sec

160 ed mouse Burkitt lymphoma (BL) harboring the shuttle vector pUR288, which includes a lacZ reporter ge

161 generated PCTs that harbored the transgenic shuttle vector, pUR288, with a lacZ reporter gene for th

162 rted into the Escherichia coli-Streptococcus shuttle vector pVA838.

163 e analysed the replication of the SV40-based shuttle vector pZ189 in four types of cells.

164 this concern, we have modified an SV40-based shuttle vector, pZ189, by exchanging the wt T-antigen fo

165 This shuttle vector, pZ402, provides us with a tool to study

166 in SV40-based replication, we constructed a shuttle vector, pZ402, that contains a mutation in SV40

167 in the CA repeat tracts of the out-of-frame shuttle vector pZCA29 and further promoted instability o

168 d two versions of an RCASBP-based retroviral shuttle vector, RSVP (RCASBP shuttle vector plasmid), co

169 Deleting part of this ORF abolishes the shuttle vector's ability to replicate in T. thermophilus

170 ound in human gastrointestinal tumors and in shuttle vector studies, where the human p53 gene-contain

171 gene in human gastrointestinal tumors and in shuttle vector studies.

172 r confirm vector integration, we developed a shuttle vector system and isolated and sequenced rAAV ve

173 n of diverse rickettsiae was achieved with a shuttle vector system based on R. amblyommii plasmids pR

174 Such a trackable E.coli /human cell line shuttle vector system capable of carrying >100 kb of gen

175 silon dA was studied using a single-stranded shuttle vector system in several E. coli strains and in

176 To study these, we used a shuttle vector system in which murine leukemia virus (ML

177 This new shuttle vector system should prove useful in characteriz

178 We developed a shuttle vector system to analyze the effect of Vpr upon

179 rt experiments in which we used a retroviral shuttle vector system to introduce and characterize targ

180 Here we have used a shuttle vector system to isolate and analyze 977 unique

181 Site-specific mutation was demonstrated in a shuttle vector system using nitrogen mustard-conjugated

182 We describe here a shuttle vector system, pBOMB4, that permits expression o

183 However, we have developed and report a shuttle vector system, pGFLEX, that provides high-level

184 Herein, we used shuttle vector technology and demonstrated that deficien

185 ed a new method employing NGS, together with shuttle vector technology, to have a multiplexed and qua

186 previously described pBSV2 as a backbone, a shuttle vector, termed pKFSS1, which carries the aadA op

187 n size in E. colicells.We describe a new PAC shuttle vector that can be propagated in both bacterial

188 approached, in which a recombinant bacmid, a shuttle vector that can be propagated in both Escherichi

189 an example we present a phagemid retroviral shuttle vector that can be used to achieve stable expres

190 uction of MIN6 cells with an adenovirus gene shuttle vector that expressed human STIM1 Immunoprecipit

191 ASP-1 in serum resistance, we also created a shuttle vector that expresses CRASP-1 from the native B.

192 A shuttle vector that is capable of replicating in Actinob

193 denticola, an Escherichia coli-T. denticola shuttle vector that renders T. denticola resistant to co

194 erase chain reaction and used to construct a shuttle vector that replicates in Escherichia coli and B

195 ucted experiments with a CpG-methylated supF shuttle vector that was irradiated with UVB and then inc

196 d for modifying BACs by using a novel set of shuttle vectors that contain the R6Kgamma origin for DNA

197 an potentially serve as a preferred host for shuttle vectors that express recombinant proteins, inclu

198 intracellular gene targeting in the episomal shuttle vector, the psoralen-PNA-induced mutation freque

199 /H2O2-induced DNA damage upon passage of the shuttle vector through human fibroblasts.

200 argeted using a novel adeno-associated virus shuttle vector to deliver microRNA-adapted shRNA via int

201 Here we have used a CpG-methylated shuttle vector to derive mutational spectra of copper/H2

202 29S6/SvEvTac strain in a bacterial/mammalian shuttle vector to facilitate functional gene studies.

203 of a genomic library in a his3(+)-containing shuttle vector to facilitate the cloning of genes by com

204 s, we have utilized a recombinant AAV (rAAV) shuttle vector to identify circular AAV intermediates fr

205 eotides were inserted into a single-stranded shuttle vector to investigate mutagenic specificities of

206 rgdorferi infectious cycle, we constructed a shuttle vector to selectively displace lp38 from the B.

207 als include the use of replication-defective shuttle vectors to deliver exogenous genes and replicati

208 ative to R. monacensis, was still present in shuttle vector transformed R. monacensis at a level simi

209 i5 as a donor virus and recombined it with a shuttle vector via a loxP site.

210 y to the introduction of currently available shuttle vectors via electroporation.

211 mus transformants replicate any newly-formed shuttle vectors via introduced thermophilic oris.

212 In our previous study, a shuttle vector was developed as a useful tool for engine

213 hen the Arabidopsis cDNA cloned in a special shuttle vector was expressed in a S. pombe mutant defici

214 were completely abolished when the adducted shuttle vector was replicated in cells lacking nucleotid

215 The shuttle vector was stable in E. coli under ampicillin se

216 Using a recombinant shuttle vector, we have demonstrated that circularized r

217 In the plasmid assays, Bacteroides-derived shuttle vectors were tested for transfer from P. gingiva

218 omers inserted into single-stranded phagemid shuttle vectors were used to transform E. coli or to tra

219 We have constructed an episomal shuttle vector which can transfer large (>100 kb) human

220 ithine transcarbamylase in a yeast/bacterial shuttle vector, which can be stably maintained in E. col

221 These targets were incorporated into oriP shuttle vectors, which replicate episomally in human lym

222 plasmid gene function, we generated plasmid shuttle vectors with deletions in each of the eight ORFs

223 cause they can be deleted from plasmid-based shuttle vectors with no apparent effect on vector mainte

224 AL1 in the multicopy yeast/ Escherichia coli shuttle vector YEpRF1.