ライフサイエンスコーパス: single channel

1 ices are not yet fully practical as they are single channel.

2 ns is not as straightforward as in CE with a single channel.

3 ay radiatively in unity quantum yield and in single channel.

4 calable throughput of 200,000 cells/min in a single channel.

5 y two landmark genes are designed to share a single channel.

6 A single-channel (2 m x 125 bps) prototype and an 8-channe

7 LSP resonance can be maximized to reach the single channel absorption limit, provided that the scatt

8 By analyzing single-channel activities at limiting calcium concentrat

9 hermore, let-7b induces TLR7/TRPA1-dependent single-channel activities in DRG neurons and HEK293 cell

10 Interestingly, single-channel activity elicited by GABA is similar to t

11 concentration of 6 mmol/L and decreased KATP single-channel activity in beta-cells of control mice bu

12 CFTR intraburst gating, we investigated the single-channel activity of human CFTR at different intra

13 ication of the NO donor NOC-18 increased the single-channel activity of Kir6.2/SUR2A (i.e., the princ

14 with purified human TRPA1 showed substantial single-channel activity only in the presence of protopor

15 P dependence of G551D-CFTR without restoring single-channel activity to wild-type levels.

16 geminal primary sensory neurons, we detected single-channel activity with biophysical and pharmacolog

17 CFTR demonstrated that both mutations have a single-channel amplitude similar to wild type (WT), and

18 Single-channel analyses indicated that the R165A mutatio

19 Single-channel analyses revealed that FMRP loss reduced

20 Single channel analysis showed that this dinucleoside pr

21 Single channel analysis, mutational studies and molecula

22 Single-channel analysis of Mg(2+)-sensitive chimeras and

23 hair cells but extend these data to include single-channel analysis, hair-bundle mechanics, and chan

24 Using single-channel analysis, we show that, even at supraphys

25 channel gating has been characterized at the single channel and macroscopic current levels, there is

26 We characterized single-channel and macroscopic GIRK1/2 currents, and sur

27 o highly efficacious (strong potentiators of single-channel and macroscopic peak responses).

28 chieving the best performance of DBP in both single-channel and multi-channel communication systems,

29 between CAF and HAL is demonstrated in both single-channel and myotube preparations.

30 ults show that the deterministic growth of a single channel based on its local environment can be use

31 Here, we investigate the single-channel behaviour of ovine CFTR and the impact of

32 lical sites in LF showed increasingly longer single-channel blockades as a function of peptide concen

33 lockers are believed to have advantages over single-channel blockers for AF, but their development ha

34 ent deactivation by decreasing or increasing single channel burst duration, respectively.

35 ind Pade (rational-function) approximants to single-channel Ca(2+) and buffer concentrations, extendi

36 tion near a point Ca(2+) source describing a single-channel Ca(2+) nanodomain in the presence of a si

37 tion near a point Ca(2+) source describing a single-channel Ca(2+) nanodomain, in the presence of a s

38 icient approximants that estimate stationary single-channel Ca(2+) nanodomains with great accuracy in

39 Single-channel cell-attached analysis demonstrated that

40 composition (alpha4beta2)2beta2 exhibit low single channel conductance and long mean open lifetime a

41 ophysical characteristics of piezo2, such as single channel conductance and sensitivities to various

42 Neither maneuver modified the single channel conductance but instead increased channel

43 Furthermore, both VDAC anion selectivity and single channel conductance increased with acidification,

44 cial phospholipid membranes, with an average single channel conductance of 0.55 +/- 0.01 nanosiemens

45 ich increased the mean open time but not the single channel conductance of the channel.

46 mechanically activated cation channel with a single channel conductance of ~28.6 pS.

47 omposition (alpha4beta2)2alpha4 exhibit high single channel conductance, brief mean open lifetime, an

48 ate each subunit stoichiometry with a unique single channel conductance, mean open channel lifetime,

49 tinguished from the homodimers by its unique single channel conductance.

50 nce regulator (CFTR), a 25% reduction of the single-channel conductance (g) and a approximately 13-fo

51 d previous findings of a 25% decrease of the single-channel conductance (g) in R117H-CFTR, but found

52 n, susceptibility to pharmacological agents, single-channel conductance and Ca(2+) permeability.

53 R proteome, acts to reduce the weighted mean single-channel conductance and calcium permeability of r

54 nnel selectivity filter dramatically reduced single-channel conductance and ion selectivity.

55 Such reduction resulted from decreased single-channel conductance and not in the number of acti

56 or affinity to endogenous agonists, modifies single-channel conductance and shapes response decay kin

57 in AMPAR channel behavior include increased single-channel conductance and weakened block of calcium

58 of Kcv, including K(+) selectivity and high single-channel conductance but reversibly photoactivates

59 The additional decrease in single-channel conductance by MTSEA(+) in five of seven

60 n and sensitivity to inhibition, and altered single-channel conductance compared to human CFTR.

61 The tandem channel has an intermediate single-channel conductance compared with the TRESK and T

62 state MS, isothermal titration calorimetry, single-channel conductance measurements in planar lipid

63 ctuation analysis (SFA), we demonstrate that single-channel conductance of AMPARs in SCs is 8-11 pS,

64 In bilayers, as well as in cells, the single-channel conductance of the complexes depends on t

65 s a change in pharmacological properties and single-channel conductance of the synaptic receptors, co

66 tural basis for gating, ion selectivity, and single-channel conductance properties of GlyR in a lipid

67 First, single-channel conductance was increased.

68 ve to calcium and caffeine, but no change in single-channel conductance was observed.

69 Single-channel conductance was reduced by four-fold in G

70 undle's resting position, (3) a reduction of single-channel conductance, (4) a change in ion selectiv

71 moval, causing changes in current amplitude, single-channel conductance, and EC50 value toward its ag

72 acteristics of their anion transport such as single-channel conductance, outward rectification, and d

73 soma of SNc DA neurons, as well as a smaller single-channel conductance, pointing to a possible diffe

74 mplitude and in the underlying weighted mean single-channel conductance, revealing that GSG1L acts to

75 it, enhances receptor function by increasing single-channel conductance, slowing channel gating, incr

76 ine in the pore-forming region increased its single-channel conductance.

77 on at the plasma membrane and increasing its single-channel conductance.

78 ivation gating and a marked reduction in its single-channel conductance.

79 ; for example, their Ca(2+) permeability and single-channel conductance.

80 conformation to alter calcium permeation and single-channel conductance.SIGNIFICANCE STATEMENT How fo

81 We investigated characteristics of single-channel conductances and opening and closing rate

82 e absence of beta1, all isoforms had similar single-channel conductances and voltage-dependent activa

83 t of mPanx1 shows outward rectification with single-channel conductances of 20 pS for inward currents

84 e three TMC variants studied have comparable single-channel conductances, although the lack of correl

85 Thus single channel current measurements reveal bases for the

86 Functional studies using single channel current recordings of the sensorless pore

87 g its open probability without modifying the single channel current.

88 With the reported method, single-channel current amplitude of native voltage-gated

89 tential of -20 mV, we found that the average single-channel current amplitude was approximately 0.04

90 ccompanied by a significant reduction of the single-channel current amplitude, indicating an interact

91 s is a method that allows measurement of the single-channel current of ion channels even when it is t

92 By using single-channel current recording, we define discrete ass

93 pore-membrane binding and discuss how to use single-channel current recordings and dye flux assays to

94 erminated, which led to the proposal that gA single-channel current transitions result from closed-op

95 mean channel open-time, open probability, or single-channel current.

96 increased opening frequency and open time of single channel currents activated by maximally effective

97 Here we compare agonist-elicited single channel currents from receptors assembled with an

98 flux) increases linearly as the amplitude of single channel currents increases, while the open time f

99 simulations and EAAT2/EAAT4 measurements of single-channel currents and anion/cation selectivities.

100 CAF +/- HAL are studied on RYR1 single-channel currents and HET myotubes to define molec

101 s contain the E160R mutation, whole-cell and single-channel currents are still observed in both the p

102 The amplitudes of their single-channel currents in a physiological concentration

103 On the basis of single-channel currents recorded from the muscle nicotin

104 In the presence of tRNA, blocking events of single-channel currents through the pore formed by recom

105 A1 (hTRPA1) into lipid bilayers and recorded single-channel currents to understand its inherent therm

106 (ii) covalent modulation of macroscopic and single-channel currents using extracellularly and intrac

107 We examined GluN1/GluN2A single-channel currents with kinetic analyses and modeli

108 Irregular voltage-dependent single-channel currents, high gating charges, and effici

109 tance that has precluded direct recording of single-channel currents.

110 Similar differences are also seen in the single-channel currents.

111 method is demonstrated on synthetic and real single-channel data from muscle nicotinic acetylcholine

112 Further kinetic modeling of the single-channel data suggested that the positive and nega

113 article, using a stochastic puff model and a single-channel data-based IP3R model, we establish the d

114 h variable near-infrared/SWIR excitation and single-channel detection, facilitating video-rate multic

115 wever, are limited due to their conventional single-channel detection: one NMR probe exclusively uses

116 f three EFCs embedded in a compact and handy single channel device and we demonstrate for the first t

117 fication of eukaryotic mRNA within a simple, single-channel device for very low RNA concentrations th

118 bridization efficiency compared to that of a single-channel device without adding any complexity to t

119 output relationship have remained inherently single-channel devices (just like their electronic count

120 s that the conformational rearrangement of a single channel directly shortens the tip link.

121 by lowering cell-surface expression, whereas single-channel effects were primarily responsible for re

122 gle-molecule FRET spectroscopic imaging with single-channel electric current recording.

123 Here, using single-channel electrical recording in planar lipid bila

124 y crystallography, native mass spectrometry, single-channel electrical recording, molecular simulatio

125 re by site-specific chemical modification in single-channel electrical recordings and gel electrophor

126 Single-channel electrical recordings of the peptide alam

127 Single-channel electrophysiological recordings in the ce

128 Whole-cell, macropatch, and single-channel electrophysiological recordings indicate

129 Single-channel electrophysiological recordings of hetero

130 Single channel electrophysiology and liposome swelling e

131 easured using liposome permeation assays and single channel electrophysiology differs significantly b

132 We also performed single channel electrophysiology measurements and found

133 ther by conventional ensemble experiments or single-channel electrophysiology method.

134 pid frog Xenopus laevis Using whole-cell and single-channel electrophysiology of Xenopus oocytes expr

135 Molecular dynamics simulations and single-channel electrophysiology reveal a 'pedal bin' me

136 ing the possibility of using this device for single-channel electrophysiology sensing applications.

137 ar the core that influence affinity, and use single-channel electrophysiology to corroborate the resu

138 functional neurotransmitter-binding site and single-channel electrophysiology to measure independentl

139 e receptors distinguish ACh and Cho, we used single-channel electrophysiology to measure resting affi

140 We used single-channel electrophysiology to measure the effects

141 We used single-channel electrophysiology to show that oAbeta(42)

142 ts of ion channel conformational change with single-channel electrophysiology would enable a direct l

143 is similar to that obtained previously using single-channel electrophysiology, and the activation cur

144 ography, molecular dynamics simulations, and single-channel electrophysiology, that CymA forms a mono

145 orded by using 2-electrode voltage clamp and single-channel electrophysiology, whereas radioimmunolab

146 ryonic kidney 293 cells using whole-cell and single-channel electrophysiology.

147 d spectroscopic analyses of heme-binding and single channel experiments identified Cys628 and His648

148 nel in a mixture, and the results agree with single-channel experiments and extended molecular dynami

149 is study, molecular dynamics simulations and single-channel experiments of VDAC-1 show agreement for

150 icant as suggested by molecular dynamics and single-channel experiments.

151 ructure can sustain ion currents measured in single-channel experiments.

152 River segments with a single channel flowing across the valley bottom export D

153 DASC)", that resolves ACs from CCPs based on single channel fluorescent movies.

154 ave established that C-family HCOs contain a single channel for uptake from the bacterial cytoplasm o

155 blies incorporating the crown ethers work as single channels for the selective transport of K(+) or R

156 d channel approach was adapted utilizing all single channels from RGB, HSV, and CieLab color space an

157 whether Tric-a gene knockout (KO) alters the single-channel function of skeletal RyR (RyR1).

158 Here, we compared single-channel gating behavior of two natural Ca(V)1.3 s

159 The detailed single-channel gating kinetics of mouse pannexin 1 (mPan

160 pore space, while the second stage follows a single-channel growth regime.

161 In symmetrical 150 mm K(+) , single channels had a conductance close to 250 pS, withi

162 ture dependence of conductance measured on a single channel in 1 M KCl shows an increase characterize

163 achieve unity radiative decay of excitons in single channel in comparison to other types of neutral l

164 mizole was still effective in blocking TRPC5 single channels in excised inside-out membrane patches,

165 iP, revealing a trimeric cylinder that forms single channels in phospholipid bilayers.

166 f the SPAC at 1.5 T compared with use of the single-channel inflatable endorectal balloon coil.

167 es on the isolated vagus and patch clamp and single-channel inside-out experiments showed that the ef

168 The transition to a single channel is consistent with the observed bottlenec

169 witching between gating modes with different single-channel kinetics and open probability.

170 nhanced CaM-dependent RyR2 inhibition at the single channel level compared with CaM-WT.

171 At the single channel level, UBP684 produced a dramatic reducti

172 conducted electrophysiological recordings on single-channel level and did not find any differences.

173 High-resolution analyses at the single-channel level showed that, when open, R1389H CaV2

174 At the single-channel level, activation by orthosteric and allo

175 However, at the single-channel level, chronic co-incubation greatly incr

176 At the single-channel level, KCNQ1 antibodies increase the open

177 At the single-channel level, MBED mimicked the agonistic action

178 At the single-channel level, oxidation of the cardiac ryanodine

179 At the single-channel level, the weak agonism is revealed by th

180 At the single-channel level, we observed reduced unitary conduc

181 Single-channel lifetime experiments, molecular dynamics

182 The single-channel lifetime was found to increase monotonica

183 The average single-channel lifetimes in the two-component bilayers (

184 This increase is most pronounced for the single-channel LZ complexity measure, and hence for temp

185 r improved detection sensitivity compared to single channel measurements and that increased signal co

186 ated by Ca(2+) and did not conduct Ca(2+) in single channel measurements.

187 G4941D did not noticeably conduct Ca(2+) in single-channel measurements.

188 ctor of up to 20 were achieved compared with single-channel measurements.

189 te' is larger than can be accounted for by a single channel model, but can be explained by coupling o

190 Single channel models propose that this occurs through t

191 Whereas current single channel models would need extending to incorporat

192 tically-controlled reverse transcription and single-channel monitoring of L-DNAs to develop a multipl

193 s novel methodology, we achieve simultaneous single-channel multiplexing and enhanced quantification

194 simulated world and are supplied with only a single channel of communication.

195 ch superior sensing behavior compared to the single-channel ones, with the capability to not only ide

196 subunit, calstabin1, and displayed increased single channel open probability (Po).

197 es reduced whole cell currents by decreasing single channel open probability without loss of surface

198 ng a slowing of deactivation, an increase in single channel open probability, or a reduction in C-typ

199 a cytosolic GOF mutation was highly active (single-channel open probability >0.3) in the absence of

200 Single-channel open probability (P(o)) of neuronal SLO-2

201 cells exhibited reduced Ca(2+) sensitivity, single-channel open probability and tamoxifen sensitivit

202 is mutation profoundly decreased the maximum single-channel open probability of homomeric GlyRs (to 0

203 s chloride sensitivity, activation rate, and single-channel open probability of SLO-2.

204 pically determined by comparing steady-state single-channel open probability or macroscopic peak resp

205 F, whereas ryanodine receptor expression and single-channel open probability were increased.

206 M but not alpha-actinin-1 binding, decreased single-channel open probability, gating charge movement,

207 ay time course of sIPSCs and potentiation of single-channel open probability.

208 e that utilizes multiple exponentials to fit single-channel open time (OT) and closed time (CT) distr

209 ryanodine-receptor channel type-2), and RyR2 single-channel open-probability were significantly incre

210 We used single-channel optical analysis of the prototypic transm

211 built on volume visualization pipelines for single channel or red-green-blue (RGB) channels have bec

212 eneration is occurring either via a dominant single channel or via both channels but with comparable

213 In contrast, GABA cannot elicit single-channel or macroscopic currents of alpha7 or the

214 d determine intermolecular interactions from single-channel or multicolor confocal laser-scanning mic

215 These first recordings of single-channel Orai1 currents reveal unexpected dynamics

216 -Orai1 fluorescence transients correspond to single-channel Orai1 responses.

217 experimentally measured correlations between single-channel OTs and CTs.

218 by STIM1, exhibit repetitive fluctuations in single-channel output.

219 Currents were measured using whole cell and single channel patch clamp techniques.

220 le-averaged spectroscopic imaging or only by single channel patch-clamp electric recording methods.

221 Here, using a mitochondrial single-channel patch clamp and cyclophilin D (CypD)-defi

222 As shown in single-channel patch clamp experiments on HEK293 cells,

223 d mutation were studied using whole-cell and single-channel patch recordings.

224 Single-channel patch-clamp and four-channel fluorescence

225 Single-channel patch-clamp recordings confirmed that the

226 Single channel permeation of the larger GSH anion was lo

227 v7 channel PMs are shown to recapitulate the single-channel permeation and pharmacological specificit

228 ing its performance to that of a calibrated, single-channel pipet in volume delivery experiments.

229 ring multiple, time-consuming motions with a single-channel pipet or device designs limited to the co

230 dress this problem using the high-resolution single-channel planar lipid bilayer technique.

231 ducting electrode, one electrical lead and a single-channel potentiostat.

232 ultaneous topography-activity mapping with a single channel probe.

233 n expression, plasma membrane insertion, and single channel properties remain unaffected, indicating

234 erations in both cell-surface expression and single-channel properties accounted for the reduction in

235 ar cation (TRIC) channels but their distinct single-channel properties and physiological regulation h

236 eract with FMRP; this interaction alters the single-channel properties of the Slack channel.

237 By electrical recordings, we measured the single-channel properties that revealed the autonomous a

238 Single channel recording indicated that coexpression of

239 Single-channel recording identified that burst length fo

240 Single-channel recording in a heterologous system reveal

241 e similar inhibitory effects on RyR2 using a single-channel recording of RyR2 activity in artificial

242 ted into lipid bilayers, and investigated by single-channel recording under various activating condit

243 Our results from fluctuation analysis, single-channel recording, and kinetic modeling, suggest

244 Here, using optical single-channel recording, we track multiple isolated ele

245 individual CFTR Cl(-) channels, we performed single-channel recording, whereas to assess entire CFTR

246 In single channel recordings (100 nM cytoplasmic [Ca(2+)] +

247 Optical and electrophysiological single channel recordings confirm that these newly inser

248 akes unambiguous interpretation of data from single channel recordings difficult.

249 nalysis of sugar translocation obtained from single channel recordings indicated that the Trp(136) mu

250 Our results are the first to demonstrate single channel recordings of piezo2.

251 Single channel recordings revealed reduced voltage/Ca(2+

252 Single channel recordings revealed the conductance of th

253 Single channel recordings showed that acidification did

254 Finally, our concentration jump and single channel recordings suggest that GluN1-GluN2B-GluN

255 nhibition of parvalbumin neurons, as well as single channel recordings, indicates that heteromeric al

256 In single channel recordings, significant differences in th

257 , as inferred by [(3)H]ryanodine binding and single channel recordings.

258 studied using rapid agonist application, and single channel recordings.

259 MET channel unitary conductance, assayed by single-channel recordings and by measurements of current

260 different Fluc channel homologues is seen in single-channel recordings as long-lived nonconducting ev

261 means of electrophysiological whole-cell and single-channel recordings as well as Ca(2+)-imaging and

262 hat the information obtained in steady-state single-channel recordings can be used to forecast modula

263 Single-channel recordings confirmed the voltage dependen

264 Single-channel recordings demonstrated a leftward shift

265 ased BK channel open probability measured in single-channel recordings from Npas1-tdTm neurons.

266 Although macroscopic and single-channel recordings have provided significant info

267 Single-channel recordings in CA3 pyramidal neurons revea

268 Moreover, single-channel recordings indicate that a previously unr

269 Single-channel recordings indicated that the GORK S722E

270 Computational methods and single-channel recordings indicated that the open G4941K

271 Kinetic analysis of single-channel recordings made with monobody on both sid

272 e, we use direct monobody-binding assays and single-channel recordings of a Fluc channel homolog to r

273 hole-cell patch clamp experiments as well as single-channel recordings on Chinese hamster ovary cells

274 Single-channel recordings reveal a significant decrease

275 Single-channel recordings show only whether the channel

276 Whole cell and single-channel recordings showed EQQ and EQQQQ to have i

277 this family, we explored by macroscopic and single-channel recordings whether the inhibitory neurotr

278 By Phi analysis based on single-channel recordings, we observed a temporal sequen

279 Using statistical criteria of the single-channel regime and pulsed excitations of the diso

280 In this single-channel regime, the disordered sample can be trea

281 ic transport (currents) can be measured with single channel resolution, imaging their structure and i

282 In cardiomyocytes, consistent with our single channel results, we show that Zn(2+) modulates bo

283 Tracking single channels revealed transient visits (~100 ms) of p

284 Based on single-channel reversal potential measurements, NMCCs ar

285 ons epitaxially grown on silicon carbide are single-channel room-temperature ballistic conductors on

286 t hemisphere (mid OTS) was consistent with a single channel, showing (i) limited spatial selectivity,

287 Comparisons of whole-cell and single-channel SLO-2 currents in native neurons and musc

288 aired with CAD association, support multiple single-channel states.

289 Single-channel studies of the read-through proteins of E

290 es of potentiation as determined in previous single-channel studies.

291 ty to examine the impact of dysfunction of a single channel subtype in detail in vivo.

292 compressing the incoming plane-waves into a single channel through the transfer function of the rada

293 gically diverse, ranging from through-going, single channels to discontinuous, multi-channelled syste

294 Single-channel translocations of a 10-residue, guest-hos

295 [Formula: see text] binding analysis and a single-channel voltage clamp were utilized to measure en

296 ngs of channel population ensembles and also single channels, we examine regulation of the Ca(2+)- an

297 Both single channels, which reveal no change in the maximum c

298 Our chip consists of a single channel with several inline pocket structures.

299 th functional studies of oppositely oriented single channels within the same lipid bilayer.

300 ent manner and reduced the mean open time of single channels without affecting their conductance, arg