ライフサイエンスコーパス: single guide RNA

1 ruption activity to the previously published single guide RNA.

2 nd lentiviral vectors that express Cre and a single-guide RNA.

3 nd is robust to artifacts of low-specificity single guide RNAs.

4 ivation complexes to target loci by modified single guide RNAs.

5 dels (in vivo); 16 websites used to generate single-guide RNA; 4 websites for off-target effects; and

6 o-associated virus 9) to deliver multiplexed single guide RNAs against Ube2v1 in cardiac-specific Cas

7 ol and optimizing the strategy for designing single-guide RNAs against circRNA BSJ sites, we markedly

8 f rice with BE3 or HF1-BE3 in the absence of single-guide RNA also results in the rise of genome-wide

9 ingle CRISPRi transgene broadly expressing a single guide RNA and a catalytically dead Cas9 fused to

10 diting techniques, including mRNA, Cas9 mRNA/single guide RNA and Cas9 ribonucleoprotein complexes, a

11 s HNH domain (AceCas9-DeltaHNH) bound with a single guide RNA and DNA substrates, one with the correc

12 The drive component, consisting of dsx single guide RNA and DsRed genes, was introduced into th

13 resence of at least one mismatch between the single guide RNA and the non-target gene sequences.

14 d protein 9 (Cas9) complexed with a specific single-guide RNA and immobilized on the transistor to yi

15 gulating HIV transcription, we used specific single-guide RNAs and endonuclease-deficient Cas9 to per

16 can be achieved by electroporation of small single-guide RNAs and ssODN repair templates alone.

17 nes, Cas enzyme variants, engineered sgRNAs (single guide RNAs) and CRISPR editing systems.

18 as9 library that consisted of around 123,000 single-guide RNAs, and profiled genes whose loss in tumo

19 ecies, including antisense oligonucleotides, single-guided RNA, and messenger RNA, in varied lipid ma

20 enables multiplexed gene activation using a single guide RNA array.

21 substantially easier to construct than Cas9 single-guide RNA arrays, facilitating multiplex genome e

22 oteins, prime editing guide RNAs and nicking single guide RNAs as transient ribonucleoprotein complex

23 d in 2011 and was quickly co-opted to create single-guide RNAs as core components of CRISPR-Cas9 tech

24 transactivated >600 LTR12C elements by using single guide RNA-based dCas9-SunTag-VP64, a site-specifi

25 crystal structure of AcrIIA4 in complex with single-guide RNA-bound SpyCas9, thereby establishing tha

26 components containing ABEmax-VRQR-SpCas9 and single-guide RNA by adeno-associated virus serotype 9 in

27 Single-guide RNAs can target exogenous CRISPR-Cas protei

28 multimodal CRISPR screens with robust direct single-guide RNA capture and to clonotype-aware multimod

29 Together with single-guide RNAs, Cas9 also functions as a powerful gen

30 adeno-associated virus serotype 6 vector and single guide RNA/Cas9 ribonucleoprotein complexes.

31 Importantly, the delivery of the single-guide RNA/Cas9 editing complex to the host cell i

32 Indeed, we show here that microinjection of single-guide RNA/Cas9 ribonucleoprotein complexes into f

33 here the Sleeping Beauty (SB) transposon and single guide RNA cassette are nested in an adeno-associa

34 SB-mediated genomic integration of the single guide RNA cassette enables efficient gene editing

35 muscle-specific Cas9 cassette together with single-guide RNA cassettes and, in one approach, a dystr

36 RISPR Associated protein 9) DNA nuclease and single guide RNA components, and differences in the rela

37 We also observed that multiple single-guide RNAs could be expressed in the same transcr

38 of genome-integrated NOT/NOR gates based on single guide RNAs (CRISPR-dCas9) to inform a Bt user con

39 moral injection of nanoparticle-based CRISPR single-guide RNA/CRISPR-associated protein 9 mRNA agains

40 Furthermore, using a single guide RNA, dCas9-SunTag-DNMT3A is able to methyla

41 cipient of Cas9 together with JCPyV-specific single-guide RNA delivered prior to or after JCPyV infec

42 A unique feature of CRISPR-Cas9 is a single-guide RNA directing a Cas9 nuclease toward its ge

43 s for correcting biases due to heterogeneous single-guide RNA efficiency, gene-independent responses

44 We packaged SaCas9 and its single guide RNA expression cassette into a single AAV v

45 This is followed by the introduction of single-guide RNAs flanking the knockin cassette to engin

46 ide an accessible data resource, predesigned single guide RNAs for targeting 3,275,697 ENCODE SCREEN

47 The single guide RNA-formed from the CRISPR RNA (crRNA) and

48 eir ability to join to specific genomic Cas9/single-guide RNA-generated bait DSBs.

49 70-83) identify a novel mechanism by which a single guide RNA hairpin can specify two uridines adjace

50 active Cas9 protein (dCas9) and programmable single guide RNAs, has emerged as a powerful genetic too

51 econdary structure onto the spacer region of single guide RNAs (hp-sgRNAs) can increase specificity b

52 r the generation of hundreds to thousands of single guide RNAs in pooled format for the production of

53 njection of mouse embryos with Cas9 mRNA and single guide RNAs induces on-target and off-target mutat

54 ssociated virus PHP.eB encoding BE3.9max and single guide RNA installing PRNP R37X resulted in 37% av

55 by co-injection of Cas9 mRNA, eGFP mRNA, and single guide RNAs into fertilized eggs.

56 microinjection of Cas9 protein and synthetic single-guide RNA into the vitellaria of young amictic (a

57 Controlled by recombination, a single guide RNA is stochastically chosen from a set tar

58 The editing directed by a single guide RNA is sufficient to impose a requirement f

59 suggests that these anti-CRISPRs manipulate single guide RNA length, loading or stability.

60 engineering human cytomegalovirus to express single guide RNA libraries directly from the viral genom

61 s9(p300) activator constructs and lentiviral single guide RNA libraries to target DNase I hypersensit

62 Using pooled lentiviral single-guide RNA libraries, we conducted a genome-wide l

63 creening using a ubiquitin regulator-focused single-guide RNA library in HL lines carrying either wil

64 Facile single-guide RNA library synthesis allows CRISPR-Cas scr

65 CRISPR) system, we constructed a genome-wide single-guide RNA library to screen for genes required fo

66 expression in human prostate cancer cells by single guided RNA-mediated targeting activated AKT and i

67 We generated a library of around 117,000 single guide RNA molecules targeting base editors to pro

68 Cas9's ability to be directed by single 'guide RNA' molecules to target nearly any sequen

69 new hypotheses, regardless of variations in single guide RNA optimization, ribonucleoprotein concent

70 Targeting of MAP4K4 with single guide RNAs or a MAP4K4 inhibitor reduced migratio

71 d selecting the optimal base editor (BE) and single-guide RNA pair (sgRNA) for a given target can be

72 Computational prediction of optimal CGBE-single-guide RNA pairs enables high-purity transversion

73 ansfection using electroporation of Cas9 and single guide RNA plasmids.

74 nctional null mutants by expressing just one single-guide RNA, reaching up to 100% editing rate in no

75 the MECP2 promoter by dCas9-Tet1 with target single-guide RNA reactivated MECP2 from Xi in RTT hESCs

76 y target HLA-DRB, -DQB1, and -DPB1 through a single guide RNA recognizing a conserved region in exon

77 S15 and the trans-activating CRISPR RNA or a single guide RNA reduces, but does not abrogate, off-tar

78 Crystal structures of Cas9 bound to single-guide RNA reveal a conformation distinct from bot

79 gineering in human CD4(+) T cells using Cas9:single-guide RNA ribonucleoproteins (Cas9 RNPs).

80 Here, using electroporation of Cas9 nuclease/single-guide RNA ribonucleoproteins and taking advantage

81 nucleic acids, functional protein, and Cas9 single-guide RNA ribonucleoproteins into both adherent a

82 R-Cas families found in nature and tailoring single-guide RNA sequences for Cas9-like effector protei

83 library methodology to simultaneously assess single guide RNA (sgRNA) activity across approximately 1

84 t DNA repair mechanism, was used to generate single guide RNA (sgRNA) activity profiles for both SpCa

85 lly inactive Cas9 (dCas9) and by providing a single guide RNA (sgRNA) against the human Alu retrotran

86 from Streptococcus pyogenes (SpCas9) using a single guide RNA (sgRNA) against the repeat tract.

87 ing guide RNAs (pegRNAs) can be split into a single guide RNA (sgRNA) and a circular RNA RT template

88 acteria Scytonema hofmanni in complex with a single guide RNA (sgRNA) and a double-stranded target DN

89 ystal structures of SaCas9 in complex with a single guide RNA (sgRNA) and its double-stranded DNA tar

90 ase bulge or up to 13 mismatches between the single guide RNA (sgRNA) and its genomic target, which r

91 ile genome-engineering tool that relies on a single guide RNA (sgRNA) and the Cas9 enzyme for genome

92 t sites can be shielded from the active Cas9*single guide RNA (sgRNA) complex through the co-administ

93 e present CRISPR/Cas9 gene editing dogma for single guide RNA (sgRNA) delivery is based on the premis

94 Using hepatic single guide RNA (sgRNA) delivery, we targeted large gen

95 and facilitating improved decision-making in single guide RNA (sgRNA) design.

96 A single guide RNA (sgRNA) directs Cas9 nuclease for gene-

97 In this system, a single guide RNA (sgRNA) directs the endonuclease Cas9 t

98 the DNA sequence features that contribute to single guide RNA (sgRNA) efficiency in CRISPR-based scre

99 Structure-guided single guide RNA (sgRNA) engineering leads to sgRNA-v2,

100 hat utilizes a ribozyme-guide-ribozyme (RGR) single guide RNA (sgRNA) expression strategy with RNA po

101 diting approach in which a Cas9 enzyme and a single guide RNA (sgRNA) form a ribonucleoprotein comple

102 a combined protein (Cas9) and an engineered single guide RNA (sgRNA) genome editing platform that of

103 Streptococcus pyogenes (spCas9) along with a single guide RNA (sgRNA) has emerged as a versatile tool

104 ntent phenotype imaging with high-throughput single guide RNA (sgRNA) identification in individual ce

105 e transcriptomes, nascent transcriptomes and single guide RNA (sgRNA) identities across hundreds of g

106 for purity evaluation of 100nt long, used as single guide RNA (sgRNA) in CRISPR technology, and promo

107 g EGFP-tagged Cas9 and lentivirus encoding a single guide RNA (sgRNA) in primary human lung microvasc

108 lished by microinjection of Cas9 DNA/RNA and single guide RNA (sgRNA) into zygotes to generate modifi

109 simultaneously delivers the Cas9 protein and single guide RNA (sgRNA) is based on DNA nanoclews, yarn

110 e strategy for cloning and sequencing paired single guide RNA (sgRNA) libraries and a robust statisti

111 ng has been constrained by the large size of single guide RNA (sgRNA) libraries and challenges in gen

112 larly Interspaced Short Palindromic Repeats) single guide RNA (sgRNA) libraries targeting entire king

113 using cytosine and adenine base editors and single guide RNA (sgRNA) libraries.

114 ese smORFs, we designed a custom CRISPR/Cas9 single guide RNA (sgRNA) library and screened for smORFs

115 The single guide RNA (sgRNA) of the system recognizes its ta

116 played multiple roles to facilitate Cas9 and single guide RNA (sgRNA) ribonucleoprotein (RNP) deliver

117 ed a bioreducible lipidoid-encapsulated Cas9/single guide RNA (sgRNA) ribonucleoprotein [lipidoid nan

118 lease-dead (d) Cas9 combined with engineered single guide RNA (sgRNA) scaffolds that bind sets of flu

119 R/Cas9 gene editing, we first identified two single guide RNA (sgRNA) sequences located on PD-L1 exon

120 nal activation in plants via engineering the single guide RNA (sgRNA) structure.

121 enes and its simplified derivative, the Cas9/single guide RNA (sgRNA) system, have emerged as potent

122 HOP is a web tool for identifying CRISPR-Cas single guide RNA (sgRNA) targets.

123 vated in vitro by CRISPR/Cas9 editing with a single guide RNA (sgRNA) that does not discriminate betw

124 vage at off-target sites in vitro, we used a single guide RNA (sgRNA) that has been previously shown

125 ed Short Palindromic Repeats system allows a single guide RNA (sgRNA) to direct a protein with combin

126 ial, the CRISPR-Cas9 system utilizes a short single guide RNA (sgRNA) to direct the endonuclease Cas9

127 R-associated (Cas)9 can be programmed with a single guide RNA (sgRNA) to generate site-specific DNA b

128 ions has been found to vary depending on the single guide RNA (sgRNA) used.

129 or DNA element) by pairing of a programmable single guide RNA (sgRNA) with a complementary sequence o

130 The assembly of single guide RNA (sgRNA) with the Cas9 protein may limit

131 hairpin (SBH) structure at the 5' end of the single guide RNA (sgRNA), which abrogates the function o

132 of robust bioinformatics tools for design of single guide RNA (sgRNA), which determines the efficacy

133 editing efficacy is mainly dependent on the single guide RNA (sgRNA), which guides Cas9 for genome c

134 moter mutation to -124C was achieved using a single guide RNA (sgRNA)-guided and catalytically impair

135 r RNA, ABE8s induce no significant levels of single guide RNA (sgRNA)-independent off-target adenine

136 a double cut HDR donor, which is flanked by single guide RNA (sgRNA)-PAM sequences and is released a

137 gions that are complementary to a programmed single guide RNA (sgRNA).

138 by programming the sequence of an associated single guide RNA (sgRNA).

139 -null Cas9 protein or to an aptamer-modified single guide RNA (sgRNA).

140 lly inactive Cas9 protein and a customizable single guide RNA (sgRNA).

141 erest by simply changing the sequence of the single guide RNA (sgRNA).

142 ells as a purified protein in complex with a single guide RNA (sgRNA).

143 KO or floxed) alleles by microinjection of 2 single guide RNAs (sgRNA) and 2 single-stranded oligonuc

144 ts performance still relies on well-designed single guide RNAs (sgRNA).

145 a single dCas9 that recognizes two different single guide RNAs (sgRNA).

146 treptococcus pyogenes Cas9 alone or bound to single-guide RNA (sgRNA) and target DNA revealed a bilob

147 inity with DNA when mismatches to the target single-guide RNA (sgRNA) are present in the sgRNA:DNA he

148 ional transitions in the solution state upon single-guide RNA (sgRNA) binding, which is a critical ea

149 encoding the adenine base editor (ABE) and a single-guide RNA (sgRNA) can correct an A>G splice-site

150 Existing single-guide RNA (sgRNA) design tools mainly depend on a

151 rt threshold distance from PAM into the Cas9/single-guide RNA (sgRNA) interior is hindered.

152 chnique is the delivery of Cas9 nuclease and single-guide RNA (sgRNA) into the specific cell and orga

153 oinjection of a mixture of Cas9 DNA/mRNA and single-guide RNA (sgRNA) into zygotes.

154 Single-guide RNA (sgRNA) is one of the two key component

155 man cytomegalovirus is engineered to express single-guide RNA (sgRNA) libraries directly from its gen

156 election that uses a genome-scale lentiviral single-guide RNA (sgRNA) library.

157 diting platform is simplified by a synthetic single-guide RNA (sgRNA) mimicking the natural dual tran

158 Unlike for single-guide RNA (sgRNA) pooled screening platforms, com

159 vesicles (EVs) as a vehicle to deliver Cas9/single-guide RNA (sgRNA) ribonucleoprotein (RNP) complex

160 We describe a cloning-free single-guide RNA (sgRNA) synthesis, coupled with streaml

161 ne, including target selection; cloning-free single-guide RNA (sgRNA) synthesis; microinjection; vali

162 ansduced with a pooled lentiviral library of single-guide RNA (sgRNA) targeting 19,114 human genes, e

163 red Cas9 activation complexes to investigate single-guide RNA (sgRNA) targeting rules for effective t

164 cles for the delivery of Cas9 nuclease and a single-guide RNA (sgRNA) that enables the controlled sto

165 Using either multiple cleavages induced by a single-guide RNA (sgRNA) that targets multiple chromosom

166 d Adeno-Associated Virus 9 (AAV9) to deliver single-guide RNA (sgRNA) that targets the Myh6 locus exc

167 Here, we modified the CRISPR single-guide RNA (sgRNA) to carry two distinct molecular

168 Similar to SpCas9, NmCas9 is able to use a single-guide RNA (sgRNA) to direct its activity.

169 gulatory sequences to constitutively express single-guide RNA (sgRNA) transcripts.

170 LNPs co-delivering mRNA (mCas9) and single-guide RNA (sgRNA) were successfully formulated an

171 nded DNA at a sequence programmed by a short single-guide RNA (sgRNA), can result in off-target DNA m

172 By simply altering the sequence of the single-guide RNA (sgRNA), one can reprogram Cas9 to targ

173 Cas9 (dCas9) protein assembled with various single-guide RNA (sgRNA), we demonstrated rapid and robu

174 The basic module consists of an inactive single-guide RNA (sgRNA)-like component that is converte

175 Sp Cas9 in the presence or the absence of a single-guide RNA (sgRNA).

176 us aureus Cas9 ortholog (SaCas9) guided by a single-guide RNA (sgRNA).

177 ites based on complementarity to a complexed single-guide RNA (sgRNA).

178 skeletal muscle myoblast cell lines using a single-guide RNA (sgRNA).

179 Using single-guide RNA (sgRNA)/dCas9 and small interfering RNA

180 using extracellular vesicles (EVs) carrying single-guide RNA (sgRNA): CRISPR-Cas9 ribonucleoprotein

181 repeats (CRISPR)-based genetic screens using single-guide-RNA (sgRNA) libraries have proven powerful

182 Phosphorothioate (PS) modifications in single-guided RNA (sgRNA) are crucial for genome editing

183 merary guanine nucleotides at the 5' ends of single guide RNAs (sgRNAs) account for diminished CRISPR

184 ounts of Cas9-encoding mRNA and multiplexing single guide RNAs (sgRNAs) allowed for phenocopy of know

185 r with bacteria expressing promoter-specific single guide RNAs (sgRNAs) also allows activation of gen

186 tion, yet defining effective combinations of single guide RNAs (sgRNAs) and base editors remains chal

187 vely on the relative abundance of integrated single guide RNAs (sgRNAs) between populations, which do

188 four, ten, and up to twenty-four multiplexed single guide RNAs (sgRNAs) can induce mutations in 90% o

189 dies have shown that the use of 5' truncated single guide RNAs (sgRNAs) can reduce the rate of unwant

190 We identify single guide RNAs (sgRNAs) capable of silencing the larg

191 Single guide RNAs (sgRNAs) designed against Xanthomonas

192 ry mouse immune cells, we used high-fidelity single guide RNAs (sgRNAs) designed with an sgRNA design

193 ort that chemical alterations to synthesized single guide RNAs (sgRNAs) enhance genome editing effici

194 recombination (HR), we designed a series of single guide RNAs (sgRNAs) flanking the mutation and pro

195 In addition, infected cells expressing single guide RNAs (sgRNAs) for both of these genes displ

196 ide access to a database of over 3.4 million single guide RNAs (sgRNAs) for iSTOP (sgSTOPs) targeting

197 tures to accurately predict highly effective single guide RNAs (sgRNAs) for targeting nuclease-dead C

198 GRIBCG identifies single guide RNAs (sgRNAs) for use with Streptococcus py

199 terspaced short palindromic repeats (CRISPR) single guide RNAs (sgRNAs) from a single RNA polymerase

200 encing by synthesis (SBS) to identify 80,408 single guide RNAs (sgRNAs) in 10,366,390 cells-over an o

201 t (CRISPR)-based knockout by analysis of 373 single guide RNAs (sgRNAs) in 6 cells lines and show tha

202 as9) from Streptococcus pyogenes loaded with single guide RNAs (sgRNAs) in mouse embryonic stem cells

203 We also create a library of single guide RNAs (sgRNAs) predicted to generate 52,034

204 ptococcus pyogenes Cas9 DNA endonuclease and single guide RNAs (sgRNAs) produced using T7 RNA polymer

205 However, variable activity across different single guide RNAs (sgRNAs) remains a significant limitat

206 Targeting SURF4 with multiple independent single guide RNAs (sgRNAs) resulted in intracellular acc

207 and +55 enhancers with 3xNLS-SpCas9 and two single guide RNAs (sgRNAs) resulted in superior HbF indu

208 Cas9 RNA-guided endonuclease, together with single guide RNAs (sgRNAs) specific for E6 or E7, is abl

209 ivering a single Cas9 enzyme and two or more single guide RNAs (sgRNAs) targeted to distinct genomic

210 In MAD-DASH, Cas9 is complexed with single guide RNAs (sgRNAs) targeting adapter dimer ligat

211 we generated a focused sublibrary containing single guide RNAs (sgRNAs) targeting hundreds of genes a

212 emarkably, we recovered multiple independent single guide RNAs (sgRNAs) targeting three different mem

213 These applications require the design of single guide RNAs (sgRNAs) that are efficient and specif

214 es within native crRNA:tracrRNA duplexes and single guide RNAs (sgRNAs) that direct Cas9 endonuclease

215 ial CTCF loop anchors in K562 cells were not single guide RNAs (sgRNAs) that disrupted gene expressio

216 performing CRISPRi with a series of mutated single guide RNAs (sgRNAs) that modulate gene expression

217 the assay by showing that the expression of single guide RNAs (sgRNAs) that target genes encoding kn

218 cells with pools of either single or double single guide RNAs (sgRNAs) to downregulate individual ge

219 ver a CRISPR plasmid DNA expressing Cas9 and single guide RNAs (sgRNAs) to the liver that directly ta

220 Three individual single guide RNAs (sgRNAs) were designed per gene to fac

221 activation unless multiple promoter-specific single guide RNAs (sgRNAs) were used.

222 tigate conformational changes of two 100 mer single guide RNAs (sgRNAs) with complex HOS and aggregat

223 Compared with single guide RNAs (sgRNAs), pegRNAs have an additional 3

224 AAV5)-packaged S. pyogenes Cas9 (SpCas9) and single guide RNAs (sgRNAs), we observed ~19%-26% Tomato-

225 293T cells, in combination with 12 different single guide RNAs (sgRNAs).

226 can be targeted to specific genomic loci by single guide RNAs (sgRNAs).

227 eening typically uses large genomic pools of single guide RNAs (sgRNAs).

228 ering high quantities of biologically active single guide RNAs (sgRNAs).

229 otides disrupting the secondary structure of single-guide RNAs (sgRNAs) and also via their combinatio

230 e strategy to integrate genes encoding Cas9, single-guide RNAs (sgRNAs) and barcoded reporter librari

231 ognition of specific DNA sequences by CRISPR single-guide RNAs (sgRNAs) and fluorescent-protein-fused

232 methods suffer from interference between the single-guide RNAs (sgRNAs) and from limited gene targeti

233 nd rigorously predicts off-target binding of single-guide RNAs (sgRNAs) and TALENs.

234 Here, we show that some single-guide RNAs (sgRNAs) can induce exon skipping or l

235 ir of dCas9-fluorescent proteins and cognate single-guide RNAs (sgRNAs) efficiently labeled several t

236 Variants of single-guide RNAs (sgRNAs) for four endogenous loci were

237 cross a genome by selecting sets of specific single-guide RNAs (sgRNAs) for labeling multiple loci of

238 To address this challenge, we design single-guide RNAs (sgRNAs) integrated with up to 16 MS2

239 A revised CBE single-guide RNAs (sgRNAs) scoring system was developed

240 Coinjection of Cas9 mRNA and single-guide RNAs (sgRNAs) targeting Tet1 and Tet2 into

241 comparable to wild-type SpCas9 with >85% of single-guide RNAs (sgRNAs) tested in human cells.

242 sitivity to BCL-X(L) inhibitor ABT-263 using single-guide RNAs (sgRNAs) that induce loss-of-function

243 However, our understanding of how to select single-guide RNAs (sgRNAs) that mediate efficient Cas9 a

244 ide library of CRISPR interference (CRISPRi) single-guide RNAs (sgRNAs) that target human pseudogene

245 ciently targeted to genomic loci by means of single-guide RNAs (sgRNAs) to enable genome editing.

246 Viral delivery of single-guide RNAs (sgRNAs) to transgenic plants that sta

247 using CRISPR interference, coexpressing many single-guide RNAs (sgRNAs) triggers genetic instability

248 enes using CRISPR interference and series of single-guide RNAs (sgRNAs) with systematically modulated

249 lindromic repeats (CRISPR)-Cas9 and pairs of single-guide RNAs (sgRNAs), but can be inefficient and i

250 ethod (SITE-Seq), using Cas9 programmed with single-guide RNAs (sgRNAs), to identify the sequence of

251 transcripts of viral RNAs (MS2 and PP7) and single-guide RNAs (sgRNAs), which when co-expressed with

252 ine using a genome-scale library with 67,405 single-guide RNAs (sgRNAs).

253 ent mixture model for the log-fold change of single-guide RNAs (sgRNAs).

254 sine stretches to the 5'-end of conventional single-guide RNAs (sgRNAs).

255 plants using RNA virus-mediated delivery of single-guide RNAs (sgRNAs).

256 ough engineering Streptococcus pyogenes Cas9 single-guide RNAs (sgRNAs).

257 s assembling thousands of vectors expressing single-guide RNAs (sgRNAs).

258 limited by reliance on indirect indexing of single-guide RNAs (sgRNAs).

259 atile systems, based on in vitro transcribed single-guide-RNAs (sgRNAs) and fluorescently-tagged reco

260 as9 (dCas9) coupled with a telomere-specific single-guide RNA (sgTelo) can be used to model the BFB c

261 study, we clone CRISPR/cas9 constructs with single-guide RNAs specifically targeting biogenesis proc

262 We leveraged the single guide RNA structure as an engineered moiety for g

263 can be completed within ~2 weeks, including single-guide RNA synthesis, early embryo microinjection,

264 study we identify an adenine base editor and single-guide RNA system that can efficiently correct thi

265 eptococcus pyogenes was pre-complexed with a single guide RNA targeting downstream of the ubiquitousl

266 d by TRBO-G-3'gGFP expressing GFP and also a single guide RNA targeting the mgfp5 gene in the Nicotia

267 ulating messenger RNA for Cas9 protein and a single guide RNA targeting TTR.

268 We developed a CRISPR screen using ~18,000 single guide RNAs targeting >700 kilobases surrounding t

269 nockout (PigGeCKO) library containing 85,674 single guide RNAs targeting 17,743 protein-coding genes,

270 ive of this, our data show the enrichment of single guide RNAs targeting 28 glycolysis-related genes

271 CRISPR activation of TTN using single guide RNAs targeting either the TTN promoter or r

272 activation, we designed a library of CRISPR/single guide RNAs targeting known and potential PIP(3)-b

273 cell leukemia (ATL) and found enrichment of single guide RNAs targeting PRDM1, which encodes B lymph

274 tion of a lentivirus expressing an ABE and a single-guide RNA targeting a de novo nonsense mutation i

275 V) vectors encoding nuclease-dead Cas9 and a single-guide RNA targeting CUG repeats results in the ex

276 based delivery of mRNA encoding an ABE and a single-guide RNA targeting PCSK9, a negative regulator o

277 Here, we design genome-scale libraries of single-guide RNAs targeting 7.2 megabases of sequence in

278 eport a method involving libraries of paired single-guide RNAs targeting both ends of an NCRE as a sc

279 Our custom library knockout screens revealed single-guide RNAs targeting Pten, Fbxw7, and genes encod

280 iated viruses expressing CRE recombinase and single-guide RNAs targeting Rpsa, we knocked out Rpsa in

281 We applied CHANGE-seq to 110 single guide RNA targets across 13 therapeutically relev

282 genome editing), a tool comprising a dynamic single-guide RNA that senses miRNA complexed with Argona

283 ssociated virus that encodes both Cas9 and a single-guide RNA that targets the mutation.

284 ce by inserting into their terminator region single-guide RNAs that activate downstream circuits.

285 n the Caenorhabditis elegans germ line using single-guide RNAs that are expressed from a U6 small nuc

286 ctive Cas9 (dCas9), VP64 transactivators and single-guide RNAs that target the Lama1 promoter.

287 p in this process is the in silico design of single guide RNAs to efficiently and specifically target

288 rmed by delivering reagents such as Cas9 and single guide RNAs to explants in culture.

289 system analog found in prokaryotes, allows a single-guide RNA to direct a CRISPR-associated protein (

290 MIC-Drop uses multiplexed single-guide RNAs to generate biallelic mutations in inj

291 gnostic nature of this workflow by utilizing single-guide RNA, transposon, and ethyl-methyl sulfonate

292 a cytomegalovirus promoter, and an optimized single guide RNA under control of a U6 promoter were del

293 ity at sites targeted by imperfectly matched single guide RNAs was observed, suggesting that while th

294 ) complex of Cas9 protein and a PR3-specific single guide-RNA was transfected into human CD34(+) hema

295 ence, and the folding stability of the whole single guide RNA, we devised a unified, physical model t

296 a previously undescribed approach involving single guide RNA, we successfully removed large genome r

297 lent edits, PE6 variants and proximal 'dead' single-guide RNAs-we increased correction efficiencies f

298 Streptococcus pyogenes Cas9 endonuclease and single guide RNAs were cointroduced with or without DNA

299 th DNA on their exteriors and preloaded with single-guide RNA were synthesized and evaluated for thei

300 nce of two sub-regions in the seed region of single guide RNA which extends the established biologica