ライフサイエンスコーパス: single molecule

1 roach that combines both drug classes into a single molecule.

2 ive and attractive forces to send or receive single molecules.

3 tantaneous displacements of freely diffusing single molecules.

4 cs now delivers sensors capable of detecting single molecules.

5 and chemical activity from the viewpoint of single-molecule 3D structure determination.

6 Here, we present a 3D single-molecule active real-time tracking method (3D-SMA

7 th healthy and disease samples, we found the single molecule activity distribution of ALP in serum re

8 ide between these approaches, we present the single-molecule adenine methylated oligonucleosome seque

9 The quasi single-molecule amplification achieved via ePCR represen

10 Using single-molecule analyses, we examined the mechanism by w

11 Single molecule analysis reveals that Bad is a processiv

12 e conversion of substrates to products using single molecule analysis.

13 CMG unloading in Xenopus egg extracts using single-molecule and ensemble approaches.

14 Over the last decade, new single-molecule and in vivo biophysical methods have all

15 c fluorescent probe (SiR-PyPDS) that enables single-molecule and real-time detection of individual G4

16 h intermolecular and intramolecular paths in single-molecule and single-stacking thiophene junctions

17 We review evidence from single-molecule and structural studies for force-induced

18 nts, and triplet excited state quenchers for single-molecule and super-resolution imaging.

19 ting temperature (HRM) analysis and use this single-molecule approach to analyze approximately 1.2 mi

20 This study employs an in vitro single-molecule approach to elucidate the mechanism of G

21 Here, we utilize two single molecule approaches, nanodisc-based planar bilaye

22 complexity and backbone flexibility require single-molecule approaches for real-space imaging.

23 Single-molecule approaches further reveal that the prote

24 u(181) concentrations with an ultrasensitive single molecule array (Simoa) approach.

25 Longitudinal plasma NfL was measured using single molecule array (Simoa) technology.

26 sma p-tau181 was measured, using an in-house single molecule array assay.

27 We used a single molecule array immunoassay and log-transformed da

28 Using a single molecule array technology, ultrasensitive immunoa

29 While digital measurement methods such as Single Molecule Arrays (Simoa), or digital ELISA, have m

30 Here, we developed a single molecule assay to directly visualize nucleation o

31 Employing single-molecule assays to analyze the functional and dyn

32 We used in vitro ensemble and single-molecule assays to assess the impact of gRNA stru

33 enabling the simultaneous, high-resolution, single-molecule assessment of chromatin states at multik

34 Single-molecule assessment of replication fork dynamics

35 ombination of live cell imaging and in vitro single molecule binding measurements, we show that tande

36 Using ensemble and single-molecule biochemistry, we show that CtIP also dra

37 r other AAA+ unfoldases, but biochemical and single-molecule biophysical studies indicate that ClpXP

38 g biosensing, single-molecule chemistry, and single-molecule biophysics, as well as in cell biology a

39 We review fluorescent single-molecule biosensors in the second part, highlight

40 3D response of single emitters directly from single-molecule blinking datasets, and therefore allows

41 The conductance through a single molecule can be measured by contacting the molecu

42 Single molecules can now be visualised with unprecedente

43 As the fields of both DNA nanotechnology and single-molecule characterisation intertwine, a feedback

44 a wide range of areas, including biosensing, single-molecule chemistry, and single-molecule biophysic

45 ategy can be used to create highly nonlinear single-molecule circuits.

46 protocol for preparing isolated cobalt oxide single molecules (Co(1)O(x)) and clusters (Co (n) O(y))

47 erization, while stepwise photobleaching and single molecule colocalization may be used to study the

48 wing for rationalizations and predictions of single-molecule conductance measurements in paramagnetic

49 Single-molecule conductivity of the longest oxa[19]helic

50 Using sequential single molecule detection stages, we demonstrate that wh

51 ng, clinical diagnostics and ultra-sensitive single molecule detection.

52 form DNA concentration, size separation, and single-molecule detection all in one platform.

53 ancements obtained routinely enable few- and single-molecule detection.

54 e performance of modern integrated circuits, single-molecule devices must be designed to exhibit extr

55 ctories without the need for solving complex single-molecule differential equations has the potential

56 Here we introduce single-molecule displacement/diffusivity mapping (SMdM),

57 he SSB-IDL fusions are readily visualized in single-molecule DNA replication reactions.

58 equipment, paving the way to cost-effective single-molecule DNA sequencing, capable of handling wide

59 nhibiting these two important enzymes with a single molecule, either in vitro or in vivo.

60 A single-molecule electret is a long-sought-after nanoscal

61 The signature of a single-molecule electret is the switching between two el

62 In vitro single-molecule experiments confirmed that yeast condens

63 However, the methodology to perform single-molecule experiments remains relatively inaccessi

64 ng of high-throughput data across a range of single-molecule experiments.

65 Here, we adapt single molecule FISH techniques to demonstrate the prese

66 We use single-molecule FISH (smFISH) and MATLAB to visualize an

67 Combining biochemical and single molecule fluorescence approaches, we show that S1

68 Here we have adapted single molecule fluorescence in situ hybridization (smFI

69 Moreover, single molecule fluorescence microscopy reveals that DNA

70 Here we have used single molecule fluorescence resonance energy transfer (

71 Using single molecule fluorescence, time-lapse TIRF microscopy

72 Here we apply single-molecule fluorescence (Forster) resonance energy

73 3, Cy5, Cy3B) are the most utilized dyes for single-molecule fluorescence and localization-based supe

74 Here, we use single-molecule fluorescence and optical tweezers studie

75 We report a parallelized, real-time single-molecule fluorescence assay for protein interacti

76 In line with previous NMR and single-molecule fluorescence experiments, we observe tra

77 Single-molecule fluorescence imaging and time-dependent

78 Live-cell single-molecule fluorescence imaging of G4s was carried

79 Earlier single-molecule fluorescence imaging of the archaeal mod

80 In this work, we employ single-molecule fluorescence imaging to investigate the

81 e gaps, we utilized quantitative multiplexed single-molecule fluorescence in situ hybridization (smFI

82 Here, we use a combination of single-molecule fluorescence in situ hybridization (smFI

83 tools, such as single-cell transcriptomics, single-molecule fluorescence in situ hybridization and h

84 Mutagenesis screens paired with single-molecule fluorescence in situ hybridization direc

85 Using confocal microscopy and single-molecule fluorescence in situ mRNA hybridization

86 Here, we used single-molecule fluorescence in-situ hybridization (smFI

87 Single-molecule fluorescence intensity analysis of CaMKI

88 an imaging scheme that correlates cryogenic single-molecule fluorescence localizations with CET reco

89 eIF5B on the ribosome recently identified by single-molecule fluorescence measurements, we determine

90 time resolutions, such as those afforded by single-molecule fluorescence measurements.

91 Traces from single-molecule fluorescence microscopy (SMFM) experimen

92 Here we use single-molecule fluorescence microscopy to observe indiv

93 Utilizing single-molecule fluorescence on Cy5 and Cy5B, transient-

94 ver three helical periods (100-130 bp) using single-molecule fluorescence resonance energy transfer (

95 tic tweezers, atomic force microscopy (AFM), single-molecule fluorescence resonance energy transfer (

96 Here, by quantifying single-molecule fluorescence resonance energy transfer b

97 extract kinetic interaction parameters from single-molecule fluorescence resonance energy transfer t

98 s structure, we were guided by findings from single-molecule fluorescence spectroscopy and molecular

99 Here, we used single-molecule fluorescence spectroscopy to track the c

100 We outline single-molecule fluorescence techniques that can identif

101 Multiplex single-molecule fluorescent in situ hybridization (smFIS

102 Atomic force microscope (AFM) based single molecule force spectroscopy (SMFS) and a quartz c

103 Single molecule force spectroscopy (SMFS) reveals that t

104 sidues out of 647 residues) when examined by single molecule force spectroscopy in vitro displays the

105 upled kinetics of ring-opening are probed by single molecule force spectroscopy, and mechanical degra

106 es to organic and inorganic substrates using single-molecule force spectroscopy (SMFS).

107 In this work, we employ single-molecule force spectroscopy and molecular dynamic

108 ions, from using as a molecular anchorage in single-molecule force spectroscopy studies of protein me

109 Here, we have used single-molecule force spectroscopy to show that the mech

110 Using single-molecule force spectroscopy, we also define the r

111 Here, we employ single molecule Forster resonance energy transfer (FRET)

112 To test this hypothesis we use single molecule Forster Resonance Energy Transfer (smFRE

113 ons at time scales overlapping with in vitro single-molecule Forster (fluorescence) resonance energy

114 ng a bulk fluorescence dequenching assay and single-molecule Forster resonance energy transfer (smFRE

115 s of MMP1 on fibrin without crosslinks using single-molecule Forster Resonance Energy Transfer (smFRE

116 Using biochemical and single-molecule Forster resonance energy transfer (smFRE

117 binding domain of human FoxP1 by integrating single-molecule Forster resonance energy transfer and hy

118 Binding analysis and single-molecule Forster Resonance Energy Transfer confir

119 Single-molecule Forster resonance energy transfer experi

120 n which unfolding properties are observed by single-molecule Forster resonance energy transfer measur

121 The use of single-molecule Forster resonance energy transfer offers

122 Using the conformation-sensitive single-molecule Forster resonance energy transfer techni

123 We fitted the histograms of single-molecule Forster resonance energy transfer values

124 observe the NHEJ synapsis by pol mu using a single molecule FRET (smFRET) assay where we can measure

125 uctural organization of these proteins using single molecule FRET and small angle X-ray scattering.

126 Single-molecule FRET analysis reveals that the riboswitc

127 Employing a single-molecule FRET assay to probe the folding status o

128 Here, we used single-molecule FRET assays with a nanodisc membrane rec

129 Here, we combine single-molecule FRET experiments and molecular dynamics

130 Single-molecule FRET experiments that observe end synaps

131 Using single-molecule FRET investigations, we show that in the

132 ed to as "fingers closing." Here, we applied single-molecule FRET to measure distance changes associa

133 dissect kinetic interaction parameters from single-molecule FRET trajectories.

134 c-based planar bilayer electrophysiology and single-molecule FRET, to address the relationship betwee

135 We show that single-molecule HRM provides insight into specific and n

136 d is particularly detrimental when analyzing single-molecule images for 3-dimensional localization mi

137 Single molecule imaging of p44/p62 complexes without XPD

138 (2020) use an innovative single-molecule imaging approach in yeast cells to measu

139 two types of nanopores were deduced from the single-molecule imaging data.

140 Furthermore, single-molecule imaging directly demonstrates that proce

141 hesized dyes are modifiable and suitable for single-molecule imaging in biological and medical scienc

142 Here, we use live-cell single-molecule imaging in human cells to determine rate

143 We demonstrated single-molecule imaging of a model tumor marker (EGFR) o

144 Using these ligands, we achieved single-molecule imaging of mu-ORs on the surface of livi

145 Single-molecule imaging shows that msp300 is associated

146 Using single-molecule imaging, we demonstrate that both conden

147 By next integrating spectrally resolved single-molecule imaging, we show that this localized dif

148 that expressing these nanobodies coupled to single-molecule imaging-amenable tags could allow superr

149 Using fluorescent single-molecule in situ hybridization, we showed that di

150 , Western blotting, and both chromogenic and single-molecule in situ hybridizations, we validated AKI

151 Here, we provide a noise-free single-molecule interaction simulation (SMIS) tool to gi

152 Here single-molecule intermolecular FRET measurements of wild

153 e strong length dependence of conductance in single-molecule junctions with the same building blocks,

154 Single-molecule kinetic analysis demonstrates that the I

155 or protein folding and unfolding, from which single-molecule kinetic and thermodynamic information ab

156 Using these single molecule kinetics, we characterize the different

157 silico methods were further employed to add single molecule level details on the potential interacti

158 uiring vibrational absorption spectra at the single molecule level, a benchmark for bulk sample chara

159 absorption spectra and chemical maps at the single molecule level, at high throughput on a second ti

160 trolled study of biomolecular complex at the single molecule level.

161 f the signal-to-background ratio at both the single-molecule level and the ensemble level in the SWIR

162 Watching reactions on the single-molecule level provides access to this otherwise

163 on and observed its dynamics directly at the single-molecule level with total internal reflection flu

164 amics and rapid detection of proteins at the single-molecule level, but also opens new avenues toward

165 ffusion of alkaline phosphatase (ALP) at the single-molecule level, in sharp contrast to the ~20% enh

166 ly labeled DNA with the T4 DNA ligase on the single-molecule level.

167 ics of Tpm1.8 on single filaments and at the single-molecule level.

168 tivity of individual Twinkle hexamers at the single-molecule level.

169 Single-molecule-level measurements are bringing about a

170 olution yet capable of activating T cells at single-molecule levels when membrane-associated.

171 ramatically altered at both the ensemble and single-molecule levels.

172 Super-resolution imaging based on single molecule localization allows accessing nanometric

173 The resolution and accuracy of single-molecule localization microscopes (SMLMs) are rou

174 Implementations such as single-molecule localization microscopy (SMLM) and minim

175 the extent to which points are clustered in single-molecule localization microscopy data is vital to

176 e designed to render large three-dimensional single-molecule localization microscopy datasets.

177 Single-molecule localization microscopy is a powerful to

178 t limitation in the advancement of live-cell single-molecule localization microscopy is the high exci

179 roves quality and conditions for regular and single-molecule localization microscopy on live-cell sam

180 Single-molecule localization microscopy with the new ten

181 We annotate the position of PopZ with single-molecule localizations and confirm its position w

182 essibility of individual chromatin fibers, a single-molecule long-read accessible chromatin mapping s

183 Single-molecule long-read DNA sequencing with biological

184 iad investigations uncovering the actions of single molecules, macromolecular structures, and integra

185 The Fe(18)Dy(6) CCC is a Single Molecule Magnet with the highest nuclearity among

186 ime to study the vibrational properties of a single-molecule magnet (SMM) incorporating Dy(III) , nam

187 f organometallic ligand for high-performance single-molecule magnets.

188 nchorage, we demonstrate the applications in single-molecule manipulation studies of the temperature

189 eans of extending the concentration range of single molecule measurements into the cellular regime wh

190 Our real time single molecule measurements of full length p53 tetramer

191 We directly compared the FRET results with single-molecule mechanical events examined by optical tr

192 We report a single-molecule mechanistic investigation into 2-cyanobe

193 tometry as an accurate, rapid and label-free single molecule method complementary to existing DNA cha

194 The single-molecule methods that we discuss provide unpreced

195 Single-molecule methods using recombinant proteins have

196 Two of these were employed in single-molecule microscopy (SMM) experiments to investig

197 Here we present live-cell single-molecule microscopy measurements indicating that

198 We designed single-molecule molecular inversion probes for target se

199 Real-time single-molecule monitoring captures folding riboswitches

200 Single-molecule motility assays show that KIF3AC moves p

201 d stopped-flow fluorescence spectroscopy and single-molecule motility assays to delineate the chemome

202 ted this computational tool against in vitro single-molecule nanoindentation of histone variant nucle

203 ndamental operational principles of the main single-molecule nanomechanical techniques, placing parti

204 pid-protein, and lipid-dye interactions with single-molecule, nanoscale resolution.

205 lk-level accessibility measurements, observe single-molecule nucleosome and transcription factor prot

206 results provide missing connections between single-molecule observations and chromosome-scale organi

207 g challenges in separations is possible with single-molecule observations of solute-stationary phase

208 We observe a single molecule of CDTa bound to a CDTb heptamer.

209 Next, we show that the affinity of a single molecule of profilin to the polyproline tracts is

210 Here, we use single-molecule optical tweezers measurements to compare

211 and properties of materials at the level of single molecules or small interacting complexes of molec

212 In this paper, we utilize SMOLM, single-molecule orientation localization microscopy, to

213 Long-read, single-molecule PacBio sequencing allows the identificat

214 dissipate acoustic energy at the level of a single molecule/particle.

215 arated by 8-15 years and used small pool and single-molecule PCR in 43 DM1 patients.

216 Single molecule photobleaching is a powerful technique t

217 Single-chromophore single-molecule photocatalysts for the conversion and st

218 cryptography, we have successfully conducted single-molecule photon-correlation experiments of 2, sho

219 Single molecule photophysical studies of these biocompat

220 novative sensing strategies that combine the single-molecule precision with the accuracy of ensemble

221 distort the fluorescent signal emitted from single-molecule probes, which rapidly degrades resolutio

222 To this end, several new platforms based on single-molecule protein-sequencing approaches have been

223 We extended a single-molecule pull-down technique previously used to s

224 ncluding chemically induced dimerization and single-molecule pulldown, we revealed that increasing th

225 atistical gamma work distribution applied to single molecule pulling experiments.

226 Based on the largest single-molecule R-loop dataset to date, we show that ind

227 Single-molecule R-loop footprinting coupled with PacBio

228 eneration sequencing techniques, such as the Single Molecule Real Time technique from PacBio and the

229 patible with both Oxford Nanopore and PacBio Single-Molecule Real-Time (SMRT) sequencing, lrTAPS dete

230 Using whole-genome single-molecule real-time (SMRT) sequencing, we characte

231 s adenine methyltransferase footprinting and single-molecule real-time DNA sequencing to natively and

232 nce gene enrichment sequencing (RenSeq) with single-molecule real-time sequencing of PacBio for 18 ac

233 Subsequent single-molecule real-time sequencing uncovers the cytosi

234 thogenicity island of 60 such isolates using single-molecule, real-time (SMRT) sequencing technology

235 can QPM line K0326Y, which is assembled from single-molecule, real-time shotgun sequencing reads coll

236 ctures for encoded fluorescence detection of single-molecule recognition events and multiplexed discr

237 s of complex molecular assemblies at or near single molecule resolution.

238 Visualising their dynamics in live cells at single-molecule resolution is essential to elucidate the

239 We tracked H2A.Z in living yeast at single-molecule resolution, and found that H2A.Z evictio

240 d six-plex detection of the STI targets with single-molecule resolution.

241 es that can be probed with nanoscale or even single-molecule resolution.

242 y complex samples heightens the relevance of single-molecule results to industrial applications.

243 bp mRNA transport deficits were confirmed by single molecule RNA FISH.

244 resolution chromatin immunoprecipitation and single-molecule RNA analysis, we found that silencing es

245 Here, we use single-cell RNA sequencing and single-molecule RNA FISH to provide a systematic molecul

246 ucleome architectures (MINA), and sequential single-molecule RNA FISH.

247 d polymers are mucoadhesive as documented in single-molecule scale (AFM), bulk solution phase (FRAP),

248 on methods that have not been studied at the single-molecule scale can be advanced, using chiral chro

249 d Rhodamine 6G by the Al-QS was driven up to single molecule sensing (femtomolar concentration).

250 in exploring its applications, ranging from single-molecule sensing to single-cell imaging, has been

251 As a result, they generally do not achieve single-molecule sensitivity, and they require two high-a

252 o detection of biological molecules offering single-molecule sensitivity.

253 patial resolution, dynamic range, or lack of single-molecule sensitivity.

254 live and fixed cells with high contrast and single-molecule sensitivity.

255 SAMOSA is a high-throughput single-molecule sequencing method that combines adenine

256 Using single-molecule/single-cell measurements, we find that i

257 a nucleotide resolution transcriptome-wide, single molecule SM-PAT-seq method.

258 and can be useful electrode substrates for (single molecule) spectroelectrochemistry research.

259 New approaches in single molecule spectroscopy and microscopy are able to

260 s a critical step towards the untethering of single molecule spectroscopy.

261 Single-molecule spectroscopy allows us to quantify the e

262 ibers that were long thought to be chains of single molecules stacked in one dimension (1D).

263 sion can achieve such compaction, but recent single-molecule studies (Golfier et al., 2020) observed

264 Some single-molecule studies have, however, suggested that th

265 This single-molecule study in a well-defined environment prov

266 STORM, laying the foundation for accelerated single-molecule super-resolution imaging of large swaths

267 Single-molecule super-resolution microscopy has develope

268 e tremendous progress, the full potential of single-molecule super-resolution microscopy is yet to be

269 which we identify here by three-dimensional single-molecule superresolution imaging.

270 Here, we used quantitative, single-molecule superresolution microscopy to study TNFR

271 The I-V curves of this single-molecule system also exhibit memristive character

272 olid-state nanopores represents a label-free single-molecule technique that may be used to measure bi

273 We developed a single-molecule technique using styrene maleic acid lipi

274 We can use single-molecule techniques - ranging from electron and f

275 nce, or be altered by, DNA-binding proteins, single-molecule techniques are increasingly employed.

276 Here, we demonstrate detection of single-molecule thiol-disulfide exchange using a label-f

277 seconds, and thereby enables time-resolved, single-molecule, through-space probing of RNA folding us

278 social information processing, which allow a single molecule to encode a diverse array of ethological

279 tial step in the scale-up of QI effects from single molecules to parallel arrays of molecules.

280 Here, we review a broad spectrum of single-molecule tools and techniques such as optical and

281 Using single molecule total internal reflection fluorescence m

282 Single molecule tracking experiments show that the loss

283 Polarization-dependent single molecule tracking was employed to simultaneously

284 Characterizing histone H2B movements by single-molecule tracking (SMT), we resolved chromatin do

285 Using the sensor together with single-molecule tracking and computational modeling, we

286 Here, we combine single-molecule tracking and super-resolution microscopy

287 outlook for super-resolution microscopy and single-molecule tracking as tools to assess condensates

288 challenges for biochemical studies that the single-molecule tracking can overcome for other insolubl

289 Single-molecule tracking of hTR at telomeres shows that

290 Finally, we use single-molecule tracking to characterize the dynamics of

291 Using single-molecule tracking, we demonstrate that HU exhibit

292 Using single-molecule tracking, we found that both translating

293 for 3-dimensional localization microscopy or single-molecule tracking.

294 Both the histogram and single-molecule trajectories reveal an unexpected high-d

295 C) that accelerates unsupervised analysis of single-molecule trajectories.

296 e buckling times for RNA and DNA revealed by single-molecule tweezers experiments, are currently lack

297 acterium ulcerans ZTP riboswitch, we apply a single-molecule vectorial folding (VF) assay in which an

298 of metalloporphyrins into the formation of a single-molecule wire in a nanoscale gap.

299 the deposit was close to that of a Co(1)O(x) single molecule with only one cobalt ion, the minimum un

300 ge collections (>3000 molecules mum(-2) ) of single molecules with nanoscale resolution remains elusi