ライフサイエンスコーパス: single step

1 ontiguous stereocenters can be prepared in a single step.

2 tion of amphiphilic gradient copolymers in a single step.

3 and structurally diverse NH-azetidines in a single step.

4 andling by preprocessing all replicates in a single step.

5 deposition and patterning are achieved in a single step.

6 for the construction of C(sp)-Si bonds in a single step.

7 nsitive detection of the aptamer target in a single step.

8 heteroaromatic units have been prepared in a single step.

9 nd amplify fluorescence signals in situ in a single step.

10 ing for the fabrication of such devices in a single step.

11 features are difficult to be identified in a single step.

12 alogue of ibogaine that can be prepared in a single step.

13 arget protein purification and labeling to a single step.

14 h a potential to meet the HHH benchmark in a single step.

15 ,3S)-beta-methyltryptophan (beta-MeTrp) in a single step.

16 ghts into their cellular concentrations in a single step.

17 h of sp7a;mstnba with high efficiencies in a single step.

18 sis of hydroxylated 1,3-cyclohexadiones in a single step.

19 bility to generate molecular complexity in a single step.

20 sion of one class of polymer to another in a single step.

21 eation of three new stereogenic centers in a single step.

22 oxidation to install three oxygen atoms in a single step.

23 from relatively simple building blocks in a single step.

24 s to cyclic vinyl ethers in high yields in a single step.

25 , and could be manufactured efficiently in a single step.

26 rosilanes, furnishing heteroarylsilanes in a single step.

27 eta-alkenylated products, respectively, in a single step.

28 ith four contiguous stereogenic centers in a single step.

29 control over a wide range of densities in a single step.

30 simple metal-comonomer exchange process in a single step.

31 G lasers to ablate and ionize particles in a single step.

32 ormation of the two new C-C bonds occur in a single step.

33 ifficult to prepare by previous methods in a single step.

34 s of interest to phospholipid membranes in a single step.

35 rial cytoplasm across the cell envelope in a single step.

36 unusual lysine-to-tryptophan crosslink in a single step.

37 ed with a simple concentric flow device in a single step.

38 e construction of multiple ring systems in a single step.

39 hat can be prepared from primary amines in a single step.

40 of 98% and a purification factor of 63 in a single step.

41 rticular, N-Boc analogue 8 was obtained in a single step.

42 apped or degraded mRNAs can be detected in a single step.

43 number of compounds from other mixtures in a single step.

44 , and antibiotic susceptibility testing in a single step.

45 rmining the average content of ferritin in a single step.

46 nary set formation, or "Merge," evolved in a single step.

47 icles and their simultaneous patterning in a single step.

48 fluorobutenes (E-HFBs) under blue light in a single step.

49 ubtropical peats, by applying either a large single-step (10 degrees C within a day) or a slow rampin

50 ient allenes has been discovered that allows single-step access to 3-functionalized indoles that usua

51 far, a multicomponent reaction that enables single-step access to complex and diverse polysubstitute

52 This reaction opens a one-pot atom-economic single-step access to pharmaceutically targeted phosphor

53 Highlights of the synthesis include single-step access to the central 1,2,4-trisubstituted p

54 edented single-step reaction pathway and the single-step acylation process is rate-determining.

55 n optimum under a given environment and many single-step adaptive ways to reach it; thus, not only sh

56 hree signaling pathways in a straightforward single-step analysis setup, without concurrent use of si

57 resonant devices (CARDs) are fabricated in a single step and unambiguously relate changes in chemires

58 We consider 7 single-step and modular screening methods for identifyin

59 From our extensive experience of single-step and multistep continuous flow synthesis, we

60 Fitness of rescued viruses was assessed in single-step and multistep growth, which revealed that th

61 We describe the simple, scalable, single-step, and polar-solvent-free synthesis of high-qu

62 ation through digital back propagation, or a single step approach based on perturbation methods have

63 yl)benzene (DMU-212) are synthesized by this single-step approach in high yield.

64 MS-NaME is a highly scalable single-step approach performed at the genomic DNA level

65 enrichment with probe-overlap (NaME-PrO), a single-step approach with broad genome coverage that can

66 The solid-phase, single-step aptasensor showed a linear range of 0.1-1000

67 In this study, we describe a solid-phase, single-step aptasensor that showed higher performance th

68 The solid-phase, single-step aptasensor was developed based on Au nanocap

69 Hence, such solid-phase, single-step aptasensors pave the path to the development

70 open air without using masks, which offers a single-step arbitrary direct patterning approach for ele

71 tains parameter estimates from raw data in a single step as opposed to many iterations.

72 The single-step assembly of supramolecular complexes contain

73 f Ru into the [Co(3)O(4)] subcluster via the single-step assembly reaction of Co(II)(OAc)(2)(H(2)O)(4

74 s of bulk, insulating Nb2O5 synthesized in a single step at relatively low temperatures suggest that

75 nables the entire process to take place in a single step, at reduced temperatures (<420 degrees C), a

76 amples at predefined intervals and enables a single-step batch correction and quantification for high

77 MgO and CoO/MgO, were shown to be active for single step bi-reforming, the combined steam and dry ref

78 However, kinetic schemes describing these single-step binding events do not explain experimentally

79 nding is often described as two independent, single-step binding events, one high-affinity and one lo

80 Solid-phase, single-step biosensors are crucial for the development o

81 Although high-performance single-step biosensors based on the principle of Forster

82 ein microarray system, Lyme Immunochip, in a single step but interpreted adhering to the standard two

83 e production of beta-carotene by 3-fold in a single step, but also to achieve 2.5-fold improvement in

84 tionality and final ring are introduced in a single step by a redox-neutral acid-catalyzed rearrangem

85 ria, and each protein could be purified in a single step by affinity chromatography.

86 econfigurable morphologies are produced in a single step by exploiting the temperature-sensitive misc

87 ce and dope with Eu(3+) these particles in a single-step by flame synthesis and directly deposit them

88 ith CH4 into liquid fuels and chemicals in a single-step catalytic process that bypasses the producti

89 1-1 produced on wheat bran was purified by a single-step chromatographic procedure.

90 present in the NCBI GenBank and allows for a single step classification of microorganisms as well as

91 Here we report that single-step codelivery of CRISPR/Cpf1 ribonucleoproteins

92 lies on instantaneous spectral cloning and a single-step, coherent field processor.

93 ating transporters and methodology for their single-step conjugation to drugs and probes, and demonst

94 ated, (b) for a specific term, the number of single step connections can be manageable, and

95 ny-guided prevention is efficient under both single-step contact tracing as well as iterative contact

96 io-electrochemical device for spontaneous or single step conversion of biomass into electricity, base

97 The direct and single-step conversion of benzene, ethylene, and a Cu(II

98 g is a multistep process, and failure in any single step could result in unnecessary morbidity and mo

99 The reaction is suitable for single-step creation of adjacent quaternary stereocenter

100 clusion compounds with target molecules in a single-step crystallization, complementing the crystalli

101 It also permits single-step deposition of films of unprecedented thickne

102 osensor is presented here for label-free and single step detection of Bisphenol A (BPA).

103 impedimetric DNA-based aptamer sensor for a single-step detection of B. anthracis spore simulant (B.

104 h selectivity, low cost, rapid response, and single-step detection.

105 ages of the two step digestion strategy over single step digestion with trypsin.

106 ted phthalimides with aryl halide provides a single step direct access of a wide range of synthetical

107 Analysis of the single-step displacements from ribosome and EF-Tu diffus

108 TTz-bridged compounds are synthesized via a single-step, double condensation/oxidation of dithiooxam

109 hermodynamic equilibrium to be achieved in a single step during intensified continuous operation, whi

110 further reduce mosaicism to levels that make single-step embryo editing in cattle commercially feasib

111 A single step extraction-cleanup procedure using salting o

112 ied BCR sequential extraction method and two single-step extraction methods based on the use of EDTA

113 h the advent of 3D printing, we predict that single-step fabrication of these devices will enable rap

114 a rich source of amine moieties was used for single-step fabrication of ultrasensitive immunosensors

115 lf the time of current protocols, still in a single-step fashion.

116 In this report, we report a facile, single-step, first-time synthesis and in-situ functional

117 ogenous glycosyltransferases, we developed a single-step fluorescent glycan labeling strategy by usin

118 time a new sensing approach carried out in a single step for Escherichia coli O157:H7 detection.

119 cFab scaffold can be easily reformatted in a single step for expression in a bacterial or mammalian h

120 the whole blood (without pretreatment) in a single step for personalized medicine.

121 targeting probes that are (18)F-labeled in a single step for PET imaging of prostate cancer.

122 se microextraction (LPME) were combined in a single step for the first time to realize simultaneous a

123 This method provides both single-step formation of the SnO2 NRs and their integrat

124 form 3,4-dihydroisocoumarin structures in a single step from 2-methyl benzoic acid derivatives.

125 tations of >90% yeast genes are created in a single step from a full-length cDNA library.

126 rbocycles with five stereogenic centers in a single step from achiral and acyclic substrates.

127 ects the formation of C-B and C-N bonds in a single step from aminoboronic esters, which are simple s

128 are obtained in moderate to good yields in a single step from readily available acetophenones, propio

129 4-Fluoropyrazoles are accessible in a single step from readily available aldehyde-derived N-al

130 spirobutyrolactone 14, was synthesized in a single step from salvinorin B and had similar potency an

131 e obtained with excellent stereocontrol in a single step from secondary anilines and aldehydes posses

132 ng access to a 2,5-dihydrofuran product in a single step from simple precursors.

133 e IFE differentiation is best described as a single step gradualistic process with a large number of

134 These lenses are easy to fabricate using single-step grayscale lithography and can be inexpensive

135 s examined in multiround transmission versus single-step growth curves, and reveal a hitherto-unknown

136 istent with the current understanding of the single-step growth cycle, HSV suppresses host DNA synthe

137 Single-step growth experiments in different cell lines d

138 tive of this study is therefore to conduct a single step GWAS (ssGWAS) on the animal polynomial coeff

139 arin) derivatives have been synthesized from single step, high yielding (82-92%) chemistry involving

140 se inhibition in cultured cancer cells using single-step, high-dose selection, these findings highlig

141 ability to develop multidrug resistance in a single step, highlights the challenges in combating this

142 to access aliphatic sulfonamides, applying a single-step hydrosulfamoylation using inexpensive olefin

143 tures were fixed using a new fast and gentle single-step immobilization technique on a hydrophobic gl

144 g so, we developed a conceptually innovative single-step immunosensing platform, avoiding blocking, s

145 hen tested on ancient genomes, outperforms a single-step imputation from genotype likelihoods, sugges

146 acid to an amine has been accomplished in a single step in the synthesis of HIV drug candidate BMS-9

147 Single-step incubation of human and mouse cells with N-T

148 ed genome engineering (CRAGE), which enables single-step integration of large, complex BGC constructs

149 In addition, a single-step integration of semiconducting/transducer pol

150 one) (THP)-PSMA, has a simplified design for single-step kit-based radiolabeling.

151 Their versatile use is demonstrated by single-step labelling of DNA, antibodies and proteins, a

152 A single-step lateral flow immunoassay (LFIA) was develope

153 molecules from an advanced intermediate in a single-step led to completely controlled introduction of

154 htforward fabrication, generally requiring a single-step lithography, and the possibility of vertical

155 This work points towards single step manufacturing of microstructured fibers usin

156 This entirely reagentless and single step marker quantification is relevant to the dri

157 ar cloning workflows, including a Cas9-based single-step marker-less and scar-less genomic integratio

158 ds, the fabrication method relies on simple, single-step, maskless tabletop laser processing of silic

159 tially promising approach to sensors for the single-step measurement of specific proteins and peptide

160 A single-step mechanism involving electron transfer from D

161 A single-step metabolite extraction with internal standard

162 Most importantly, this is a single-step metal chalcogenide formation process that is

163 The single-step method coupled with the super-sensitive AID

164 We demonstrate this single-step method experimentally in the visible optical

165 Here, we introduce a facile single-step method in which bicontinuous interfacially j

166 Recently, we reported on a single-step method integrating recombinant protein purif

167 rates the super-sensitive AID system and the single-step method provides a powerful tool for elucidat

168 scription factors, we previously developed a single-step method to generate glutamatergic neurons fro

169 multiple sets of chromosomes, we utilized a single-step method where CRISPR/Cas9-based gene knockout

170 Here we present a single-step method, named REPLACR-mutagenesis (Recombine

171 rmers onto the core of an optical fiber in a single step, mimicking the 'campanile' near-field probes

172 A single-step Mo-flux controlled nucleation and growth pro

173 Our data support a single-step model of HCV IRES recruitment to 40S subunit

174 The single-step modification of GSPE offered a simple, ultra

175 ) resulted in no treatment discontinuations; single-step (n = 5) and double-step (n = 24) dosing enta

176 ids and panicoids took place via independent single-step nested chromosome fusion events after the tw

177 ed inertial microfluidics technology enables single-step neutrophil isolation (>90% purity) without i

178 ed to read out lineage relationships after a single step of clonal barcoding.

179 binders from oligonucleotide libraries in a single step of partitioning in which protein-bound and u

180 ellagitannins from wine was simplified to a single step of solid phase extraction (SPE) using size e

181 that signal integration does not occur at a single step of the pathway.

182 anosheet hybrid was synthesized via a facile single-step one-pot hydrothermal method.

183 plex samples, such as serum) and reversible, single-step operation.

184 e we address these limitations by developing single-step optimized inducible gene knockdown or knocko

185 was possible to reduce sodium by 10% in one single step or to replace 30% of NaCl by KCl without a n

186 try processes are currently often limited to single steps or short sequences due to the complexity in

187 hain reaction (PCR) for the toxin gene(s) or single-step PCR on liquid stool samples have the best te

188 lecular cloning procedures to a single-tube, single-step PCR, performed in <2 hours from setup to tra

189 Previously, this artifact was measured using single-step photobleaching.

190 g-term engraftment and could therefore allow single-step prenatal treatment of congenital hematologic

191 The single-step preparation method for phosphaviologens desc

192 Combination of the three single steps (preparation of the organometallic compound

193 In cytosensors fabrication simple single-step procedure was used which includes coating pe

194 al the presence of large macromolecules in a single-step procedure.

195 C with nickel-NTA was successfully used as a single step process for the purification of miraculin fr

196 ed 3D printer nozzle and directly drawn in a single step process.

197 rgy consumption, increases productivity in a single-step process (lower capital and operating costs)

198 reported here can be easily synthesized in a single-step process and also exhibit outstanding sensing

199 Under mild conditions, the single-step process starts as a carbene O-H insertion re

200 is understanding, we show how to employ this single-step process to produce layered structures in sof

201 es, by using a simple, lithography-free, and single-step process.

202 challenging transformations not observed in single-step processes, and to encourage application of t

203 Here, we demonstrate a facile single-step processing route for the development of bimo

204 Single-step production from a Good Manufacturing Practic

205 s were evaluated as potential substrates for single-step production.

206 The on-MALDI-target sample preparation is a single-step protocol with high derivatization efficiency

207 ng allows quantifying multiple analytes in a single step, providing advantages over individual testin

208 The single step pyrolytic synthesis was simple to perform wh

209 drolysis of ghrelin follows an unprecedented single-step reaction pathway and the single-step acylati

210 The single-step reaction pathway for the acylation is remark

211 This is the first time demonstrating that a single-step reaction pathway is possible for an ester hy

212 ng altered reaction pathway, compared to the single-step reaction, constitute the key advantages of t

213 ized using a one-pot hydrothermal method via single-step reaction.

214 These redefined modules can be used for single-step reactions or in multistep processes, where m

215 Kinetic studies of single-step reactions suggest that following invader bin

216 vel reagent was prepared on 200-g scale in a single step, reacts in the title reaction under mild con

217 The GNPs/rGO composite was synthesized by a single-step reduction of graphene oxide and HAuCl4 solut

218 tails an electrochemical methodology for the single-step reduction of TPPO to TPP using an aluminum a

219 In addition, a single step rephasing of the UK10K panel, using rare var

220 We found that in single-step replication experiments, the final yields of

221 A single-step sample treatment, for the simultaneous extra

222 ary with phage display and used to develop a single-step sandwich-type noncompetitive immunocomplex a

223 A quantitative, single-step, self-assembly of a shape-persistent monomer

224 -1.00 and specificity was 0.92-1.00; and for single step: sensitivity was 0.86-0.92 and specificity w

225 e we demonstrate a new class of reagentless, single-step sensors for the detection of proteins and pe

226 Using this method, a single-step separation factor up to 261 was obtained for

227 to form right-handed Z-DNA structures with a single-step sharp kink.

228 tion and Cas13-based detection to occur in a single step, simplifying assay preparation and reducing

229 rapid triplet pair formation in the case of single-step singlet fission.

230 Through single-step solid-state reactions, a series of novel bic

231 s into an organic semiconductor matrix via a single-step solution processing deposition of a two comp

232 say, capable of providing CDI diagnosis in a single-step solution.

233 res and surface charges were developed via a single-step spinning process without any post-treatment.

234 d palladium nanoparticles (Pd-BNP) catalyzed single-step, stereoselective domino synthesis of symmetr

235 A single-step, stereoselective protocol for the synthesis

236 hy for the print of the features, and only a single-step surface pretreatment is needed.

237 sing a monodomain magnetoelectric state with single-step switching is desirable.

238 90 degrees in-plane Co moment rotation upon single-step switching that is reproducible for hundreds

239 A single-step synthesis of 4-hydroxy-functionalized bi-ary

240 Low-cost, single-step synthesis of hollow nanotubes of N-doped car

241 A novel single-step synthesis of positively charged Pd NPs and o

242 omplex minerals and could be exploited for a single-step synthesis of van der Waals heterostructures,

243 ods of preparing Ga-ZSM-5 and reveal direct (single step) synthesis leads to optimal catalysts compar

244 The direct, single-step, synthesis is based on repetitive branching

245 The versatile single-step synthetic method uses a preformed aminoisoin

246 ic iron oxide nanoparticles (MNPs) through a single-step synthetic route involving the simultaneous n

247 In this work, a single-step, system agnostic nonlinearity compensation a

248 e conversion (DNMC) has been recognized as a single-step technology that directly converts methane in

249 These operations are completed in just one single step, thanks to the physical computing with Ohm's

250 he acquisition of the dimerization domain, a single step that can in principle account for the diverg

251 c single-element structures synthesized in a single step that generate electrical current when altern

252 O(2), this protocol allows to insert, in one single step, the desired carbon tag into carboxylic acid

253 of inexpensive common materials applied in a single step, the present scalable approach eliminates ma

254 Surprisingly, we find that during a single step, the TH stochastically hops multiple times b

255 After a simple single-step thermomechanical programming process, the fi

256 tural RNAs (rRNA, tRNA) of both species in a single step, this approach enables analysis of very low-

257 The methodology allows in one single step to immobilize, with precise control of surfa

258 functionalized expanded [11]helicene and its single-step transformation into two structurally and fun

259 Here, we are reporting a single-step transformation of N-protected alpha,beta-uns

260 lts reported here demonstrate the facile and single-step transformation of N-protected alpha,beta-uns

261 Although single-step transformations in continuous-flow systems a

262 A mild, operationally simple, and single-step transition-metal-free protocol for the synth

263 est (QFT-GIT) (2-step TST/QFT); and third, a single-step TST plus QFT-GIT (TST/QFT).

264 LTBI diagnoses decreased with the single-step TST/QFT (26.5%) compared with the 2-step TST

265 (4, 5), whereas "potential inversion" (i.e., single-step two-electron transfer; 8-10) is changed to s

266 ndritic block copolymers to be prepared in a single step under ambient conditions.

267 nd one C-X (X = C, N, O) bond formation in a single step under uniform reaction conditions.

268 e corresponding C(sp(3))-CF(3) products in a single step using a bench-stable, commercially available

269 FOS was purified in a single step using gel filtration matrix, Bio-Gel P2.

270 e hDelta29-3-ABHD6 variant was purified in a single step using Immobilized Metal Affinity Chromatogra

271 alpha-amino sp(3) carbon-hydrogen bonds in a single step, using isotopically labeled water (D2O or T2

272 dily available materials, and generate, in a single step, valuable building blocks that would be diff

273 We present a single-step, versatile strategy for the selective modifi

274 istically, this transformation proceeds in a single step via a concerted Meisenheimer-type transition

275 oxidation state side chains introduced in a single step via a late-stage double Stille coupling.

276 appropriately modified powerful penultimate single-step vindoline-catharanthine coupling strategy th

277 Herein, we report an advantageous single-step, wash-free, and real-time bacterial detectio

278 into lateral flow devices, using a scalable, single-step, weaving-based manufacturing approach.

279 ly substituted aziridines were obtained in a single step when enolates of alpha-bromo esters were emp

280 ite membranes were prepared through a facile single-step wire-wound rod coating procedure in which in

281 ining P-C, P-Si, P-Ge, and P-P bonds using a single step with a base-stabilized phosphorus atom.

282 ectly recovered from highly viscous CEW in a single step with a high yield of 94.3% and a purificatio

283 fibres (Tg = 567 degrees C) were drawn in a single step with high aspect ratios (>10(4)); such nanow

284 bered carbo- and heterocyclic molecules in a single step with high efficiency and perfect atom econom

285 to fused heterocyclic lactone scaffolds in a single step with moderate functional group tolerance and

286 f two contiguous tertiary stereocenters in a single step with moderate to high levels of diastereocon

287 The process occurs in a single step with no Mg(2+) ion activating the nucleophil

288 site-selective protein/DNA conjugation in a single step with outstanding selectivity and efficiency.

289 tify 15 different mycotoxins from maize in a single step with satisfactory linearities (0.986-0.999),

290 ociation of the stable complex occurred in a single step with the activation energy independent of te

291 ne system was evaluated to extract REEs in a single step with the feed and strip solutions circulatin

292 pectively, delivers 2-aroylbenzoxazoles in a single step with yields up to 85%.

293 CeO(2):Eu(3+) nanocrystals are combined in a single-step with larger, non-responsive Y(2)O(3):Tb(3+)

294 ting opportunity to tailor dosage forms in a single-step with minimal excipients and operations.

295 function X OR (Y NOR Z) to be performed in a single-step with three memristive switches.

296 be smoothly reduced to vicinal diamines in a single step, with high chemoselectivity.

297 fy the major NPC building blocks - each in a single step, with native elution and, as demonstrated, i

298 g from 1 to 10(7)CFU/10mL were detected in a single step without any preprocessing.

299 jugation proceeds rapidly at neutral pH in a single step without removing any charges.

300 D graphene preparation and patterning into a single step without the need for wet chemical steps.