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1 are formed from intramolecularly folded RNA single strands.
3 is initiated with end resection, leading to single-stranded 3' ends, which require microhomology ups
4 e CTL weakens the driving forces for forming single-stranded active polymers and suppresses lateral a
5 bias primarily arises during the ligation of single-strand adapters during library preparation, and t
6 nant CinB has nuclease activity against both single-stranded and double-stranded DNA but not RNA unde
7 zymes were active on modified mC residues in single-stranded and double-stranded DNA in vitro, while
8 s mostly on the DNA structure containing the single-stranded and duplex DNA junction with the allowed
9 the 2CNqA fluorophore has a quantum yield in single-stranded and duplex DNA ranging from 10% to 44% a
12 he SRAP domain to interact with a variety of single-strand- and double-strand-containing DNA structur
14 ribe a new set of elegant tools based on the single strand annealing DNA repair system and CRISPR tec
16 damage taking advantage of Rad51-independent single-strand annealing (SSA) assays in the yeast Saccha
19 resection, whereas RNF8 is not required for single-strand annealing repair involving extensive end r
20 omologous recombination mechanisms including single-strand annealing; however, POL32-dependent break-
21 scale, we investigate binding of the E. coli single stranded binding protein to two DNA oligos both i
22 rae specific to chromosome 2 (Chr2) also has single-strand binding activity in the AT-rich region of
24 tified mutations affecting the mitochondrial single-strand binding protein (SSBP1) in 4 families with
25 dentified heterozygous missense mutations in single-strand binding protein 1 (SSBP1) in 5 unrelated f
26 s mutations in nuclear-encoded mitochondrial single-strand binding protein 1 (SSBP1) in patients with
28 validated with experimental data in terms of Single Strand Break (SSB) yields for plasmid and Double
29 e type IA family, they do so by generating a single-strand break in substrate DNA and then manipulati
32 f individuals with hereditary defects in DNA single-strand break repair; a process regulated by poly(
33 1-beta-d-arabinofuranosyl cytosine) to trap single strand breaks that are formed during nucleotide e
34 but remains sensitive to DNA damage, such as single strand breaks, the most frequently-occurring lesi
36 at PARP inhibition will impair the repair of single stranded breaks, causing synthetic lethality in t
37 PNKP is implicated in the repair of both DNA single-strand breaks (SSBs) and DNA double-strand breaks
39 ere, we show induction of oxidized bases and single-strand breaks (SSBs), but not direct double-stran
41 enzymatic activity and traps PARP1 on DNA at single-strand breaks, leading to replication-induced DNA
48 DNA bound snugly between two dimers in which single-stranded casposon end binding resembles that of s
51 ection of two gRNAs, Cas9 protein and a long-single-stranded-circular DNA donor vector (lsscDNA, 6.7
52 r RNAs (circRNAs) are a distinct category of single-stranded, covalently closed RNAs formed by backsp
53 ured the surface coverage for thiol-modified single-strand deoxyribonucleic acid (ssDNA) as anchored
55 deno-associated viruses (AAVs) are typically single-stranded deoxyribonucleic acid (ssDNA) encapsulat
56 application for precise genome editing using single strand DNA oligonucleotides and show that an effi
57 ination with paper-based electrodes, using a single strand DNA relative to H1047R (A3140G) missense m
58 becomes activated and degrades a fluorescent single stranded DNA (ssDNA) reporter present in the assa
59 ) and Minute Virus of Mice (MVM), both T = 1 single stranded DNA viruses, and Bromo Mosaic Virus (BMV
61 red in several cancer genomes were linked to single-strand DNA (ssDNA) intermediates in various proce
62 lex DNAs before and after cleavage of the 5' single-strand DNA (ssDNA) tail by the AdnA nuclease.
63 one Zf-GRF motif mediating interaction with single-strand DNA (ssDNA), whereas the major AP endonucl
66 DNA excision by Exo1, which causes increased single-strand DNA formation, RPA exhaustion, DNA breaks,
68 ocesses after cisplatin treatment, including single-strand DNA repair and Fanconi anemia pathways, su
69 nduced by CRISPR/Cas9, as well as individual single-strand DNA scissions induced by the nickase versi
71 he absence of dNTPs, this leads to excessive single-strand DNA that exceeds the protective capacity o
72 the entropy associated with straightening of single-strand DNA upon nucleoprotein filament formation.
75 eation rate of ScDmc1 results from its lower single-stranded DNA (ssDNA) affinity, compared to that o
78 he AID/APOBEC enzymes deaminate cytosines in single-stranded DNA (ssDNA) and play key roles in innate
79 replication by excising oxidized bases from single-stranded DNA (ssDNA) and unhooking interstrand cr
80 ere, three different methods that can detect single-stranded DNA (ssDNA) are utilized to identify the
81 The XPA protein functions together with the single-stranded DNA (ssDNA) binding protein RPA as the c
82 d-specific excision that begins at a distant single-stranded DNA (ssDNA) break and proceeds back past
83 hemically, human RAD51 polymerises faster on single-stranded DNA (ssDNA) compared to double-stranded
85 f a DSB, controlling the formation of the 3' single-stranded DNA (ssDNA) filament needed for recombin
87 dimeric protein that potentially binds with single-stranded DNA (ssDNA) in a manner similar to human
88 n is a highly dynamic process that generates single-stranded DNA (ssDNA) in the genome as 'transcript
90 induces the generation of large stretches of single-stranded DNA (ssDNA) intermediates that are rapid
92 In the marine environment, only a few lytic single-stranded DNA (ssDNA) phages have been isolated an
94 ceeds via binding of RPA, RAD51, and DMC1 to single-stranded DNA (ssDNA) substrates created after for
97 t of DNA-RNA hybridization and the displaced single-stranded DNA (ssDNA), have been identified in bac
98 ) for the label-free electrical detection of single-stranded DNA (ssDNA), in-solution- and on-chip-hy
99 ins engineered for large-scale production of single-stranded DNA (ssDNA), we probed the substrate spe
100 py structure of human CST bound to telomeric single-stranded DNA (ssDNA), which assembles as a decame
103 is a bifunctional enzyme that contains both single-stranded DNA (ssDNA)-specific nuclease and motor
107 le-stranded DNA-binding proteins (SSBs) bind single-stranded DNA and help to recruit heterologous pro
108 e Pif1, which tracks in a 5'-3' direction on single-stranded DNA and plays a role in genome maintenan
112 ivity, which is alleviated by binding of the single-stranded DNA binding protein, RPA, to the exclude
113 ic target sites and report that simultaneous single-stranded DNA break formation at donor and accepto
115 J and RAP80 exposed to MMC are attributed to single-stranded DNA created by Mre11 and CtIP nucleases.
116 erfacial potential changes were measured for single-stranded DNA detection with an unprecedented zept
117 ki2-like nucleic acid helicase that provides single-stranded DNA for alkylation damage repair by the
118 nthesis downstream of the lesion, creating a single-stranded DNA gap that is repaired primarily in an
121 vely charged DNA binding protein J guide the single-stranded DNA genome into the icosahedral capsid.
122 tructures of Pf4 with and without its linear single-stranded DNA genome, and studied Pf4 assembly int
123 geminiviruses (plant viruses with circular, single-stranded DNA genomes) are the major group of emer
125 which long distance binding (25 angstrom) of single-stranded DNA in an allosteric site primes the act
126 nded sites and also at AT-rich regions where single-stranded DNA is exposed during origin opening.
128 nvolves introducing multiple, short pairs of single-stranded DNA overhangs to components of the struc
129 nthesized oligonucleotides or amplicon-based single-stranded DNA probes and validated the technique o
130 relies on the immobilization on a surface of single-stranded DNA probes that bind complementary targe
131 d complex thereafter nonspecifically cleaves single-stranded DNA reporter probes labeled with a fluor
133 esection of broken ends to generate long, 3' single-stranded DNA tails, annealing of complementary se
134 res formed by an RNA:DNA hybrid and unpaired single-stranded DNA that represent a source of genomic i
138 s endocytose Pf, and internalization of this single-stranded DNA virus results in phage RNA productio
141 nt R-loops (RNA-DNA hybrids with a displaced single-stranded DNA) create DNA damage and lead to genom
143 ES acts at replication forks, binds PCNA and single-stranded DNA, and generates a DNA-protein crossli
144 NA) could be used as a magnified analogue of single-stranded DNA, and that two meta-DNAs that contain
145 They exhibit 5' exonuclease activity on single-stranded DNA, hydrolyzing it at the acidic pH ass
146 an entry site for Exo1 in vitro to generate single-stranded DNA, which would be susceptible to both
147 A Polymerase Cofactor), preferentially binds single-stranded DNA, yet contains a structural fold dist
148 ted to a handful of microorganisms for which single-stranded DNA-annealing proteins (SSAPs) that prom
150 CST (CTC1-STN1-TEN1) proteins, which form a single-stranded DNA-binding complex, localize at stalled
151 on between Escherichia coli RNase HI and the single-stranded DNA-binding protein (SSB) in this proces
153 of core subunits of LMO2 and LDB1 as well as single-stranded DNA-binding protein (SSBP) cofactors and
154 overcome by the DNA-binding activity of the single-stranded DNA-binding protein RPA, efficient DNA r
156 107Q amino acid changes in the mitochondrial single-stranded DNA-binding protein, a crucial protein i
158 eavage can be rapidly digested by a 3' to 5' single-stranded DNA-specific exonuclease, indicating Cas
164 egrases innovated from double-strand- toward single-strand-DNA recombination through the acquisition
168 However, because of saturation and because a single-stranded donor DNA replaces the original allele,
171 obases predominantly occurs when DNA becomes single-stranded during replication and transcription.
173 In this report, we examined the roles of single-stranded exposed guanosines at NC binding sites i
174 eq) to generate a high-resolution profile of single-stranded genomic RNA inside viral particles.
175 res show how CHMP1B first polymerizes into a single-stranded helical filament, shaping membranes into
176 n and action appear to occur on the entering single strand in the recipient, early in conjugal transf
178 MTases yield promiscuous m6A modification of single strands, in the context SAY (where S = C or G and
179 DNA origami, in which a long template DNA single strand is folded by many short DNA oligonucleotid
181 ally, and much to our surprise, we also find single-stranded loops of minimal G4s within individual L
182 y to an RNAG-quadruplex structure that lacks single-stranded loops, double-stranded stems, or junctio
186 abditis elegans protein related to the human single-stranded nucleic acid-binding protein Pur-alpha,
188 sly described cytidine deaminases operate on single-stranded nucleic acids(3), their use in base edit
190 insulators, and semiconductors all within a single strand of fiber, an entire world of functionality
194 generation of simple knock-in alleles using single-stranded oligodeoxynucleotide (ssODN) repair temp
196 and deletion events, maintains or increases single-stranded oligodeoxynucleotides mediated precise g
199 metry, were used to show that binding of the single-strand oligonucleotide d[TTAGGGTTAG] to POT1 is b
200 broblasts, which were edited with TALENs and single-strand oligonucleotide to introduce a P663-L663 s
201 e precisely incorporate exogenously supplied single-stranded oligonucleotide donor (ssODN) at the DNA
202 e demonstrate the dual sgRNA approach with a single-stranded oligonucleotide donor is highly specific
203 the cell cycle, are present predominantly as single-stranded oligonucleotides and are suitable substr
204 in three K-12 E coli strains using multiple single-stranded oligonucleotides containing homological
205 e generated in Escherichia coli using mutant single-stranded oligonucleotides to target PCR-derived l
206 ic acid (PNA) scaffolds for the detection of single-stranded oligonucleotides, in particular microRNA
209 rchitecture comprised of a T7 promoter and a single-stranded overhang domain (ss-dsDNA), can unlock d
210 s via T-junctions formed by hybridization of single-stranded overhangs on the linkers with the loops.
211 nces of all lengths and types (5' and 3') of single-stranded overhangs, if present, on each DNA fragm
213 uding dengue virus and Zika virus, contain a single-stranded positive sense RNA genome that encodes v
216 s a specialized machinery that converts long single-stranded precursors into small RNAs of ~25-nucleo
217 unfolding and POT1 binding to the resultant single-strand provided an excellent quantitative fit to
218 and in human A549 cells, appending a 3'-end single-stranded pyrimidine (3'-ssPy) can strongly enhanc
219 pare the impact of two different methods for single-strand recovery on the efficiency of aptamer enri
221 subunits bind sequence-nonspecifically to a single-stranded region and, on breathing of the inhibito
223 l tisB standby site consists of the expected single-stranded region, but surprisingly, also a 5'-end
225 olving Watson-Crick base pairing between the single-stranded regions of a bulged helix and a hairpin
226 protein that nonspecifically recognizes the single strand RNA (ssRNA) of viral or nonviral origin.
228 et al. demonstrate that Piezo1 in ECs senses single-strand RNA (ssRNA) from intestinal microbiota to
232 report the asymmetric reconstruction of the single-stranded RNA (ssRNA) content in one of the three
233 Viruses in the Tombusviridae family have single-stranded RNA (ssRNA) genomes with T=3 icosahedral
234 aded from the periphery, TLR7 detected viral single-stranded RNA and triggered immune responses that
236 structures of apo-D1D2 and post-unwound D1D2:single-stranded RNA complex, and the structure of a D2:d
237 sslinks to specific regions characterized by single-stranded RNA flanked by structured elements and t
238 eceptor 8 (TLR8) recognizes pathogen-derived single-stranded RNA fragments to trigger innate and adap
240 Leviviruses are bacteriophages with small single-stranded RNA genomes consisting of 3-4 genes, one
243 ch is valid with a culturable positive-sense single-stranded RNA human virus, coxsackievirus B5, by a
244 of stroke-inducible tRF-22-WE8SPOX52 using a single-stranded RNA mimic induced down-regulation of imm
245 glia of live zebrafish brains, we found that single-stranded RNA of bacterial origin acts as a PAMP a
246 A bacteriophage Phi6, and the positive-sense single-stranded RNA poliovirus (PV) and human rhinovirus
247 isms that are different from the established single-stranded RNA recognition mode of PPR motifs.
249 KH0, KH1, and KH2 domains bind weakly to the single-stranded RNA sequences suggesting that they may h
250 tures known for their modular recognition of single-stranded RNA sequences with each motif in a tande
251 Here we demonstrate that wild-type FUS binds single-stranded RNA stoichiometrically in a length-depen
252 i YbeY exhibits endoribonuclease activity on single-stranded RNA substrate but not on the double-stra
255 proach that accurately tracks positive-sense single-stranded RNA virus inactivation without relying o
258 ) susceptibility compared with other enteric single-stranded RNA viruses (e.g., Echovirus 12, feline
261 ite the threat to human health posed by some single-stranded RNA viruses, little is understood about
262 ic arthropod-borne viruses (arboviruses) are single-stranded RNA viruses, the most common of which in
264 st resiquimod (R848; a synthetic analogue of single-stranded RNA) or saline by nasal spray to healthy
266 se testes, most piRNAs are derived from long single-stranded RNAs lacking annotated open reading fram
273 The decay pathway is independent of specific single-stranded sequences, as regulation is maintained i
275 e results establish YbeY as a zinc-dependent single-strand specific endoribonuclease that functions i
277 tivates CMGs to form helicases that encircle single-strand (ss) DNA and initiate bidirectional forks.
278 y member proteins can deaminate cytosines in single-strand (ss) DNA, which restricts human immunodefi
281 in of clusters are lesions in long tracts of single-stranded (ss) DNA damaged by apolipoprotein B mRN
283 We found that the addition of mitochondrial single-stranded (ss) DNA-binding protein both influences
288 ZIKV 3'UTRs occur predominantly in unpaired, single-stranded structural context, thus corroborating e
289 fers DNA or RNA substrates with TTGT or UUGU single-stranded tails and that the KH domain is critical
292 e, the complementarity of the invader to the single-stranded toehold provides the free energy bias of
293 RNA, we use antisense oligos to hybridize to single-stranded transcripts exposed on the surface of hu
294 chestrated by the Z ring, which is formed by single-stranded treadmilling protofilaments of FtsZ.
296 he first PCR cycle, cannot be corrected with single-strand UMIs and impose fundamental limits to UMI-
298 discerning details such as helical grooves, single-strand versus double-strand crossovers, backbone