ライフサイエンスコーパス: single-turnover

1 THI4p is a suicide enzyme undergoing only a single turnover.

2 h respect to that of the enzyme do involve a single turnover.

3 he sulfide source and is inactivated after a single turnover.

4 verts Cas9's activity from multi-turnover to single turnover.

5 sation of glycine with succinyl-CoA during a single turnover.

6 on yeast, we provide evidence that Dip1 is a single-turnover activator of Arp2/3 complex in vivo, rev

7 ted, the reconstituted enzymes have in vitro single-turnover activities that are 30-120% of that of t

8 sive in the context of a nucleosome, and its single-turnover activity is reduced approximately 500-fo

9 associated with antigen processing (TAP) by single-turnover analyses at single-liposome resolution.

10 Here, using ensemble single turnover and equilibrium experiments, as well as

11 ic step size of TraI was measured under both single turnover and pre-steady-state conditions.

12 the accumulation of three intermediates in a single turnover and the decay of the third is rate-limit

13 G TrCel5A from Trichoderma reesei under both single-turnover and "steady state" conditions.

14 Using single-turnover and multiple-turnover kinetic analyses,

15 enzyme-substrate complex was analyzed using single-turnover and multiturnover kinetic methods.

16 ructural processes in human ribosomes during single-turnover and processive translation reactions.

17 lysis assays, nucleotide-binding assays, and single-turnover and steady-state GTPase assays, we demon

18 er rates we have established a quantitative, single turnover approach to evaluate substituent and sol

19 o hitherto existing steady-state assays, our single-turnover approach uncovers the power stroke in su

20 Using a single-nanoparticle single-turnover approach, we study the redox catalysis o

21 We investigated radical reduction during a single turnover Arg hydroxylation reaction catalyzed by

22 An in vitro real-time single turnover assay for the Escherichia coli Sec trans

23 Analysis of ATP hydrolysis through single turnover assays indicates a surprising substrate

24 Importantly, single turnover assays revealed that the reaction was co

25 Single turnover assays showed that the ATPase activity o

26 In addition, we use pre-steady-state, single turnover assays to directly measure the rate of h

27 Single turnover assays with DHHC2 and DHHC3 demonstrated

28 In single turnover assays, (BIP)(2)B only inhibited unwindi

29 Light-initiated, single-turnover assays have been carried out with the pe

30 Pre-steady-state single-turnover assays on wild-type 9 degrees N polD wer

31 in-BGTP complex decay by 199 -778% in pseudo-single-turnover assays.

32 n steady-state ATPase activity, a lag in the single-turnover ATPase time course, and no DNA packaging

33 that the RIIbeta holoenzyme could undergo a single turnover autophosphorylation with adenosine triph

34 ar Fe(III) concentration, being limited to a single turnover by inefficient product release from the

35 The single-turnover catalytic rate constants and Michaelis c

36 ter the observed rate constants of PRORP2 in single-turnover cleavage assays.

37 cleavage 16- to 23-fold, as measured by the single-turnover cleavage rate constant.

38 kinetics, equilibrium binding energies, and single-turnover cleavage rates of mouse AGO2 RISC.

39 USP2 using stopped-flow florescence under a single-turnover condition.

40 xtracellular K(+) application to EAAC1 under single turnover conditions (K(+) inside) resulted in out

41 Using(18)O-labeled water under single turnover conditions and native high resolution pr

42 Under single turnover conditions in the presence of excess con

43 Under single turnover conditions in the presence of excess enz

44 t sigmoidal kinetics are observed only under single turnover conditions suggests that this conformati

45 Under single turnover conditions with limiting tRNA, aminoacyl

46 version of androstenedione to estrone (under single turnover conditions) generated a progress curve s

47 ce the E.coli trp repressor from dsDNA under single turnover conditions, although the substrate is un

48 a cyt P450:cyt b(5) molar ratio of 1:1 under single turnover conditions, cyt P450 2B4 catalyzes the o

49 In DNA unwinding assays under single turnover conditions, the mutant NS3h exhibited a

50 ter 12-bp substrate is unwound rapidly under single turnover conditions.

51 tics with respect to ATP concentration under single turnover conditions.

52 RGS proteins, when assayed in solution under single turnover conditions.

53 A dienolate by solvent-derived protons under single turnover conditions.

54 ompetence and was studied under multiple and single turnover conditions.

55 tant (K(D)) and the Michaelis constant under single-turnover conditions (K(M)*), and those in the (+)

56 by using resonance Raman spectroscopy under single-turnover conditions indicate that an initial brid

57 Kinetic analyses under single-turnover conditions revealed that I-LtrI activity

58 However, under single-turnover conditions that are commonly employed fo

59 binding constant of MPG toward Hx, but under single-turnover conditions there is apparently no effect

60 ed oligodeoxyribonucleotide substrates under single-turnover conditions using rapid-quench flow techn

61 Single-turnover conditions were applied, wherein phospho

62 deuterium isotope effect was observed under single-turnover conditions when CF-DXP was incubated wit

63 change the rate of substrate cleavage under single-turnover conditions, indicating that phosphorylat

64 Under single-turnover conditions, the intermediate formed with

65 Under single-turnover conditions, the ratios of branched to li

66 ed by stopped-flow kinetic experiments under single-turnover conditions.

67 using the rapid quench-flow technique, under single-turnover conditions.

68 ely 500 nM for glucosamine 6-phosphate under single-turnover conditions.

69 examined in various base pair contexts under single-turnover conditions.

70 higher rate than with wild-type mutase under single-turnover conditions.

71 , bacterial translation) under multiple- and single-turnover conditions.

72 drial encoded precursor nad6 t-element under single-turnover conditions.

73 DNAs are unwound with lower amplitudes under single-turnover conditions.

74 ) 1H and 31P{1H} NMR and ESI-MS evidence for single-turnover conversion of 2 into 1, (d) observation

75 Single-turnover cytochrome c reduction data linked these

76 or PheH was determined by global analysis of single-turnover data in the reaction of PheHDelta117, a

77 Our binding and single-turnover data support a conformational rearrangem

78 imeric PvuII endonuclease, we have collected single-turnover DNA cleavage data as a function of Mg(II

79 ssile and nonscissile strands on the rate of single-turnover DNA transesterification and the cleavage

80 , we use FRET to examine such changes during single-turnover EF-G-dependent GTPase on vacant ribosome

81 kinetic constants for Cu(I) turnover and for single-turnover electron transfer from Cu(I) to the enzy

82 ssembled cytochrome b(6)f complexes exhibits single-turnover electron transfer kinetics comparable to

83 erein, we address these open questions using single-turnover electrophysiology, time-resolved step-sc

84 ndonuclease from Streptococcus pyogenes is a single-turnover enzyme that displays a stable product st

85 mation and that the pyrimidine synthase is a single-turnover enzyme.

86 Inteins are single turnover enzymes that splice out of protein precu

87 or tight substrate binding because they are single turnover enzymes with covalently linked substrate

88 Thus, calcium can mediate a single turnover event with either ATP or adenosine-5'-(b

89 Single-turnover excision kinetics showed that in additio

90 of hypoxanthine by AAG but has no effect on single-turnover excision.

91 chytrium limacinum (designated AlACR1) under single-turnover excitation exhibited biphasic decay, the

92 as 44 s(-1) (burst experiment) and 48 s(-1) (single-turnover experiment).

93 Single turnover experiments at alkaline pH have revealed

94 Single turnover experiments reveal that the rate of tran

95 In single turnover experiments using plasmid substrates, I-

96 Single turnover experiments utilizing substrate 1 gave f

97 Single turnover experiments were conducted using 4'-amin

98 In single turnover experiments with NOHA, NO forms only in

99 Single turnover experiments with substrate 1A produced a

100 1)) substrates, much slower than k(max) from single turnover experiments, confirming that AP-DNA rele

101 Heavy LDH has slowed chemistry in single turnover experiments, supporting a contribution o

102 tically active in steady-state catalysis and single-turnover experiments and displays the same regios

103 and measurements of catalysis were taken in single-turnover experiments by observing the visible abs

104 ady-state kinetic isotope effects (KIEs) and single-turnover experiments further confirm that slow st

105 Single-turnover experiments further showed that both the

106 However, when cell extracts were tested in single-turnover experiments in vitro, where redox protei

107 Single-turnover experiments of this reduced CntB species

108 Surprisingly, single-turnover experiments revealed that Fpg-catalyzed

109 Single-turnover experiments show that the rate-limiting

110 It is also shown in single-turnover experiments that the DHP Fe(IV)O interme

111 ue, we performed engineered footprinting and single-turnover experiments to determine where and how S

112 This was further confirmed by single-turnover experiments using radiolabeled [(32)P]A5

113 Pre-steady state burst and single-turnover experiments using radiolabeled [1-(14)C]

114 Single-turnover experiments were performed in the presen

115 igh-pressure NMR reactor (WiHP-NMRR) enables single-turnover experiments with active mixing; such exp

116 In single-turnover experiments, very small amounts of DHD w

117 We further investigated processivity using single-turnover experiments, which show that SNAREs can

118 Single turnover flash experiments and biochemical analys

119 city on Q(A)(-) reoxidation kinetics after a single turnover flash, S-state transitions, and O(2) rel

120 Photoactivation using single turnover flashes revealed D1-D170H and D1-D170V,

121 e delivery of electrons to PSI in saturating single-turnover flashes) showed a strong correlation in

122 ystem II reaction centre is excited by three single-turnover flashes.

123 earlier studies by others, we have developed single-turnover fluorescence stopped-flow methods that a

124 Using single-turnover fluorescence stopped-flow methods, here

125 C2394A mutation did not decrease the rate of single-turnover GTP hydrolysis, the >20-fold increase in

126 Single turnover GTPase assays reveal that R9AP co-locali

127 rnover assays, FTIR spectroscopy depicts the single turnover GTPase reaction without nucleotide excha

128 ) release is essentially rate-determining in single-turnover GTPase.

129 oupled to helicase movement, we measured the single turnover helicase translocation-dissociation kine

130 This is the first single-turnover imaging of a molecular catalyst by fluor

131 our method, we systematically determined the single-turnover incorporation kinetics of all four nativ

132 We describe a method for measuring the single-turnover incorporation kinetics of non-fluorescen

133 the burst amplitude and k(pol), the rate of single-turnover incorporation.

134 Unlike conventional single-turnover intermediate trapping experiments, these

135 In depth single turnover kinetic analysis revealed that H285D is

136 Single turnover kinetic analysis revealed the Holliday j

137 good as the archetypal HJ resolvase RuvC in single turnover kinetic analysis.

138 y-state kinetic analysis, and the k(chem) in single turnover kinetic analysis.

139 clease has been analyzed in steady-state and single turnover kinetic assays and in equilibrium DNA bi

140 Finally, single turnover kinetic assays identify DNA binding as t

141 Using single turnover kinetic assays, we found that HIV-1 WT R

142 Multiple and single turnover kinetic data support a mechanism where a

143 onducted steady-state, pre-steady-state, and single turnover kinetic evaluation of OGG1 in alternate

144 Single turnover kinetic experiments reveal that the reac

145 he enzyme to catalyze the complete reaction, single turnover kinetic experiments were performed.

146 Here we show, using single turnover kinetic methods, that monomers of PcrA a

147 nsfer to tRNA(Tyr) has been determined using single turnover kinetic methods.

148 Steady-state and single turnover kinetic studies of these variants, combi

149 Single turnover kinetic studies on a construct where the

150 Direct measurement in single turnover kinetic studies show that pyrophosphate

151 Single turnover kinetic studies showed that CTP is incor

152 perchlorate in acetonitrile were measured in single turnover kinetic studies.

153 The single-turnover kinetic analysis also establishes that t

154 Using single-turnover kinetic analysis, we found that I174S ex

155 Single-turnover kinetic assays showed that Pol lambda bi

156 We used steady state, pre-steady state, and single-turnover kinetic assays to show that the multiple

157 Single-turnover kinetic data for the E288K variant show

158 ia coli has been studied by steady-state and single-turnover kinetic experiments for the 1-deoxy-d-xy

159 Unexpectedly, single-turnover kinetic experiments of the coupled PRODH

160 We have also applied single-turnover kinetic experiments with spectroscopic d

161 By contrast, steady-state and single-turnover kinetic measurements indicate that bioti

162 In single-turnover kinetic measurements, multiphasic reacti

163 Employing single-turnover kinetic methods, we examined human DNA p

164 Single-turnover kinetic parameters were obtained for the

165 nts, both the pre-steady state burst and the single-turnover kinetic parameters were similar to those

166 Single-turnover kinetic studies indicate that CmlA is fu

167 Moreover, single-turnover kinetic studies revealed that while POP5

168 Remarkably, single-turnover kinetic studies show that the enzyme cat

169 For MeP substitutions at the +1 position, single-turnover kinetic studies showed that the activati

170 In single-turnover kinetic studies, we show that the Y955C

171 Single turnover kinetics for the aminoacylation of tRNA(

172 It was found that the single turnover kinetics of the parent zeolite crystal p

173 Analysis of single turnover kinetics revealed a transition state int

174 se variants as recombinant proteins and used single turnover kinetics to characterize their abilities

175 Here, we apply transient state, single turnover kinetics to investigate the ATP-dependen

176 riants by antibiotic susceptibility testing, single turnover kinetics, and RNA affinity-binding assay

177 he k(cat) was 1.8 +/- 0.1/s as determined by single turnover kinetics, and the K(m) was 0.20 +/- 0.07

178 namics of OGG1 mutants were characterized by single-turnover kinetics and stopped-flow kinetics with

179 e post-transfer editing activity in LeuRS by single-turnover kinetics demonstrates that the rate-limi

180 In this article, single-turnover kinetics have been measured on a series

181 Real-time single-turnover kinetics reveal size-, catalysis-, and m

182 The specificity constants determined using single-turnover kinetics showed that uracil and hypoxant

183 nd steady-state experiments, we show here by single-turnover kinetics that minihelices are insufficie

184 We used single-turnover kinetics to characterize the reactions o

185 gation of the mechanism of inhibition, using single-turnover kinetics with polymerase in excess of DN

186 rlie sequence discrimination by MED1, we did single-turnover kinetics with the isolated, recombinant

187 Single-turnover kinetics, fluorescence anisotropy, and s

188 Here, using ThrRS as an example, rapid single-turnover kinetics, mutagenesis, and solvent isoto

189 Using a combination of steady-state and single-turnover kinetics, we probe substrate association

190 ydration (pK(a) = 7.6) are needed to fit the single-turnover kinetics.

191 ivated rate of P(i) release were measured by single-turnover kinetics.

192 Analysis of a single turnover L-Arg hydroxylation reaction revealed th

193 py, kinetic simulation, isotope entrainment, single-turnover labeling ((10)B/(2)H), and density funct

194 een 1 (lambda(max) = 440, 611, 747 nm) under single-turnover-like conditions at -78 degrees C leads t

195 rod) provide more convincing high-resolution single-turnover mapping results and clearly prove the tw

196 Single-turnover maximal rates of reaction are similar wi

197 d by the catch mechanism, were determined by single turnover measurements on myosin-bound ADP.

198 ent to catalysis along with steady state and single turnover measurements.

199 3B, and performed bisulfite sequencing-based single-turnover methylation analysis on both complexes.

200 Kinetic analysis of single-turnover nick sealing by ChVLig-AMP underscored t

201 methods to analyze the kinetic mechanism of single-turnover nick sealing by EcoLigA-AMP.

202 py was used to study heme transitions during single turnover NOHA reactions.

203 Based on steady state and single-turnover nuclease assays, we have assigned the ra

204 We collected time courses for single turnover nucleotide incorporation reactions over

205 Pre-steady-state single-turnover nucleotide incorporation assays were per

206 The single turnover of (1R)(+)-camphor-bound oxyferrous cyto

207 ent state, the maximum rate constant for the single turnover of DHT (k(trans)) was determined to be 0

208 s oxide reductase (N2OR) that is observed in single turnover of fully reduced N2OR with N2O.

209 spanning the HRM-containing tail or after a single turnover of heme at the core.

210 te rapidly produces an incipient core from a single turnover of iron, upon which subsequent Fe(II) is

211 mation of E.S reaction intermediate during a single turnover of the catalytic cycle appears to provid

212 The amounts of glycine formed after single turnovers of different preformed binary complexes

213 in tarantula leg muscle fibers by observing single turnovers of the fluorescent nucleotide analog 2'

214 d using a luciferase readout to evaluate the single-turnover PCSK9 proteolytic event.

215 that the iron remains ferric throughout this single turnover "peroxide shunt" reaction.

216 The single turnover pH dependence of pre-tRNA cleavage revea

217 Single-turnover pre-steady-state kinetics also showed th

218 Kinetic measurements on single-turnover processes in laccase established fast ty

219 ivity and a 2 x 10(-2) s-1 fast phase during single-turnover processing.

220 Binding analysis indicates that this single-turnover property is caused by tight binding to n

221 ffect of E6APC820A on wild-type activity and single-turnover pulse-chase kinetics.

222 Using tRNAs that are proflavin labeled and single-turnover rapid kinetics assays, we identify one o

223 The single turnover rate of 4-CB and ATP to form 4-CB-AMP an

224 is-309 to Asn and Asp-210 to Ala reduced the single turnover rate of incision 5 ' to AP sites by at l

225 However, the maximal single turnover rate of the FEN EXO reaction also yields

226 rts of the human enzyme, indicated a reduced single turnover rate.

227 However, the single-turnover rate constant for alkylation does depend

228 contrast with the steady-state reaction, the single-turnover rate constant for dansyl-GCVLS alkylatio

229 ,N(6)-ethenoadenine (epsilonA) from DNA with single-turnover rate constants that are 2.9 x 10(5)-fold

230 Single-turnover rate constants were slowed for heavy PNP

231 on 5 ' to AP sites is so fast that a maximal single-turnover rate could not be measured using rapid m

232 he R258A mutant exhibited an increase in the single-turnover rate of correct nucleotide insertion.

233 s the human enzyme seems to restore both the single turnover rates and narrow distribution of fast dy

234 rates reduced Km and increased multiple- and single turnover rates of endonucleolytic hydrolysis at n

235 aps of 30 nucleotides or fewer at comparable single-turnover rates.

236 G1,6bisP (12% at approximately 0.1 s) in the single turnover reaction carried out with excess betaPGM

237 Presteady state single turnover reaction data indicated that the second

238 The single turnover reaction initiated by adding dioxygen to

239 A single turnover reaction is observed with this ligand th

240 Single turnover reaction rates reveal a salt-dependent i

241 However, when the single-turnover reaction between (18)O2-P and NH2-CAM is

242 The concentration dependence of the single-turnover reaction further reveals that the glutam

243 ples recovered at defined time points in the single-turnover reaction indicate that styrene oxide syn

244 Here, mutagenesis combined with single-turnover reaction kinetics demonstrate that point

245 in the rcf1Delta and rcf2Delta strains, the single-turnover reaction of CytcO with O2 was incomplete

246 stigated the kinetics of ligand binding, the single-turnover reaction of CytcO with O2, and the linke

247 dent reactions, but this variant catalyzed a single-turnover reaction producing a 0.8:1 ratio of prod

248 e diiron site allows for 50% of a productive single-turnover reaction.

249 In contrast, established rates for single turnover reactions between purified MtrC and Fe(I

250 Single turnover reactions carried out with betaPGM, [(14

251 Single turnover reactions of [(14)C]betaG1,6bisP with ex

252 Single turnover reactions of [14C]ATP and phosphate, car

253 The kinetics of single turnover reactions of the two intermediates with

254 Subsequent single turnover reactions were monitored spectrophotomet

255 examined the reactive form of 2-KPCC and its single turnover reactions with a suicide substrate and C

256 of HPPD accumulates two transient species in single turnover reactions with the native substrate HPP.

257 ation and N-hydroxyarginine (NOHA) oxidation single turnover reactions, and in the O(2) reactivity of

258 are somewhat smaller than those obtained in single turnover reactions.

259 Single-turnover reactions at 0 degrees C gave styrene ox

260 Furthermore, pseudo-single-turnover reactions between catalytically relevant

261 into a bisthiocarboxylic acid species by two single-turnover reactions in which sacrificial desulfuri

262 Single-turnover reactions of N2OR for Cu(Z) and Cu(Z)* p

263 Single-turnover reactions of the first and second half r

264 Single-turnover reduction of cytochrome c by CPR (k(max)

265 e further show that electron transfer during single-turnover reduction of O2 is limited by proton tra

266 ubstrate concentration and operate in a near single-turnover regime in vitro.

267 easured the spectral changes associated with single-turnover reoxidation by O(2) of substrate-reduced

268 s of three different sizes in real time with single-turnover resolution, we observe clear size-depend

269 We demonstrate that single-turnover ribosome-dependent EF-G GTPase proceeds

270 delayed to the third flash during a train of single-turnover saturating flashes, the pattern of O2 em

271 In this work, a detailed 3D single molecule, single turnover sensitive fluorescence microscopy study

272 copy has been used to image and characterize single turnover sites at catalytic surfaces, but is rest

273 ity of R14-RBD/GL on RGS4 is not apparent in single-turnover solution GAP assays with purified Gialph

274 both receptor-stimulated GTPase activity and single turnover, solution-based GAP assays suggested a c

275 that can be farnesylated by FTase only under single-turnover (STO) conditions.

276 tion to the carboxylases was investigated in single turnover stopped flow and quench flow measurement

277 Single turnover stopped-flow absorbance experiments indi

278 the transfer reaction was investigated using single turnover stopped-flow and quench-flow assays.

279 Single turnover stopped-flow experiments were used to id

280 (nNOSox) have been similarly investigated by single-turnover stopped-flow and rapid-freeze quench EPR

281 te of the flavin intermediate (Dks = 2.3) in single-turnover stopped-flow experiments using (R)-[2-2H

282 Single-turnover stopped-flow kinetics experiments demons

283 Single turnover studies confirm that the reaction of the

284 Single turnover studies on HIV reverse transcriptase sug

285 Here we report single turnover studies that determine and compare the k

286 n (L-Arg --> NOHA or NOHA --> citrulline) in single turnover studies, but the W66F mutant showed a 2.

287 However, rapid mix single-turnover studies showed that T201A T4moH had a fa

288 Consistent with an "iso" mechanism, single-turnover studies with dGTP and 8-oxo-dGTP hydroly

289 The single-turnover time courses, measured for the first par

290 d was also observed using a minimal in vitro single-turnover transcription assay, revealing that this

291 the ligand binding domain using an in vitro single-turnover transcriptional termination assay, compl

292 uterium kinetic isotope effects (KIEs) using single turnover transients shows 2- to 4-fold increase i

293 eaction to synchronize the ribosome during a single-turnover translation.

294 both protein molecules in the dimer during a single turnover, traversing from the FAD domain of one m

295 mbination of site-specific DNA footprinting, single-turnover unwinding assays, and unique fluorescenc

296 transfer by intact cells, using turnover and single turnover voltammetry.

297 Correlation analyses of single-turnover waiting times further reveal activity fl

298 of the arylated enzyme intermediate during a single turnover was measured for wild-type and Glu232Asp

299 the (D)k(lim) (ratio of the maximum rates of single turnover) were 1.06 and 2.06, respectively.

300 Steady-state activity, regiospecificity, and single-turnover yields were also determined for the T201