ライフサイエンスコーパス: site specificity

1 f multiple protein substrates with excellent site specificity.

2 curs in vivo with biological selectively and site specificity.

3 provides a molecular explanation for H3K4me3 site specificity.

4 ues in APOBEC3F that might affect its target site specificity.

5 r no direct influence over V(D)J recombinase site specificity.

6 iles and correlates integrase phylogeny with site specificity.

7 id-phase peptide synthesis (SPPS) with total site specificity.

8 ccessibility may be the prime determinant of site specificity.

9 tranded RNA (ssRNA) that explains Sm binding-site specificity.

10 endonuclease-like domain and generally lack site specificity.

11 emplate, and the P157S mutation altered this site specificity.

12 ing of the hole transport dynamics with base-site specificity.

13 ly alter Ty1 RNA or protein levels or target site specificity.

14 design and selection of proteins with novel site specificity.

15 play important roles in determining binding site specificity.

16 association of specific subunits determines site specificity.

17 o effect upon defensive rage, indicating its site specificity.

18 was no increase in OT release, demonstrating site specificity.

19 the vector terminal repeats with no apparent site specificity.

20 lacks these loops, correspondingly lacks AP site specificity.

21 that cofactor binding contributes to target site specificity.

22 olutionary pathways towards acquiring target site specificity.

23 erol protocol quantifies cholesterol binding site specificity.

24 at the flanks of cognate motifs helps drive site specificity.

25 ping DNA sequence preferences achieve target site specificity.

26 include an RNA binding domain that provides site specificity.

27 rminant for serine-threonine phosphorylation site specificity.

28 within the complex for maximal activity and site specificity.

29 h regard to allosteric activation and active site specificity.

30 ere we examined the molecular basis for this site specificity.

31 differentiation genes may dictate P element site specificity.

32 the ZI or LH had no such effect, indicating site specificity.

33 ismatches near the cleavage site relaxes the site specificity.

34 nctional compromise between pH tolerance and site specificity.

35 an important role in cleavage efficiency and site specificity.

36 alyzing the requirements for RNase J1 target site specificity.

37 he protein surface one at a time with single-site specificity.

38 display enhanced reaction rates and cleavage site specificity.

39 ects and conjugation strategies with greater site-specificity.

40 roperties of CRISPR-Cas integrases including site-specificity.

41 d enabling the prediction of their insertion site specificities.

42 horylate the two proteins but with different site specificities.

43 To further investigate the nature of the site specificity a set of deletion mutants of the 160 bp

44 nts exhibit similar gel mobility and binding site specificity, although a consensus binding site comm

45 id not display clear evidence of DNA binding site specificity, although some binding site preference

46 ce on host health and intriguing patterns of site specificity amidst the lack of dispersal limitation

47 Considerable diversity in DNA binding site specificity among family members is predicted, and

48 Despite the difference in cleavage-site specificity, an increasing number of cellular prote

49 and heterodimers with different DNA binding site specificities and DNA binding affinities.

50 The site specificities and hence functional differences betw

51 encodes a DNA adenine methylase with unusual site specificity and a homopolymer tract that functions

52 ly highly robust but continue to lack active-site specificity and are challenging to rationally impro

53 nance (ssNMR) spectroscopy to quantify, with site specificity and atomic resolution, the orientation

54 d in the generation of HE variants with high site specificity and cleavage activity, to enable genome

55 a single step to overcome restricted active site specificity and confined coordination space at CUS.

56 Here, we examine insertion site specificity and global insertion behavior of two mi

57 The site specificity and integrity of the cis-syn thymine di

58 adaptability to various bacterial hosts, its site specificity and its efficiency.

59 rPAM rankings correlate strongly with target-site specificity and knock-in efficiency, validating its

60 es provide opportunities to establish target site specificity and mechanisms of antivirals.

61 This form of habituation exhibits site specificity and requires protein synthesis because

62 oth 3'-guanine bases are necessary to impart site specificity and slow dissociation kinetics with the

63 ansaminase, undergoes changes in both active site specificity and subcellular localization to switch

64 ith altered population structures, decreased site specificity and temporal stability, and colonizatio

65 Based on its Glu-tRNA primer binding site specificity and the location within the PTN gene, w

66 regulators differ in both their DNA-binding site specificity and their sensory domains.

67 ESA isoforms vary greatly in their levels of site specificity and this is likely to be determined by

68 from CTCF to BORIS was suggested to regulate site specificity and timing of epigenetic reprogramming.

69 mics (diGly) to elucidate the ubiquitylation site specificity and topology of PARKIN-dependent target

70 mechanisms governing PP2A dephosphorylation site specificity and tumor suppressor function.

71 revealed that IS1630 exhibits unusual target site specificity and, upon insertion, duplicates target

72 We find strong site-specificity and a hierarchical mechanism for both p

73 PPR proteins, which are factors required for site-specificity and enzymatic editing.

74 ning amino acid into proteins with exquisite site-specificity and homogeneity, overcoming issues asso

75 tabase also serves in the study of integrase site-specificity and its evolution.

76 lysis by LanC cyclases that control both the site-specificity and the stereochemistry of the conjugat

77 approaches are limited by the availability, site specificity, and cross-reactivity of antibodies.

78 exert control over chain topology, substrate-site specificity, and downstream physiological effects t

79 ated by IkappaBalpha, change its DNA-binding site specificity, and endow it with new transactivation

80 (24 of 35 designs, 69%) had the intended new site specificity, and that 14 of the 15 attempted specif

81 contained the recombination synapse and that site specificity, and therefore directionality, is deter

82 bodies provide a good balance of resolution, site-specificity, and proteoform heterogeneity to charac

83 How this site specificity-and, by extension, the myriad effects o

84 mputational techniques predicting TF-binding site specificity are frequently unreliable.

85 The motions, resolved with site-specificity, are dynamically heterogeneous and exhi

86 lower mobility complex with the same binding site specificity as HAF1, was also investigated.

87 Cleavage site specificity as well as the ordered action of prothr

88 ional significance of this variable cleavage site specificity as well as the relative pathological im

89 CAAAT) reveals shared determinants of target site specificity as well as variations in the protein-DN

90 ST25, ST115, ST124, and ST126) demonstrated site specificity, as they were significantly (P < 0.05)

91 than mammals is due in large part to target site specificity at the corresponding nicotinic acetylch

92 Site specificity at this second level is less well under

93 ontribute to the distinct substrate cleavage site specificities between the two proteases.

94 and viral communities, which showed not only site specificity but also individual signatures.

95 transposase proteins that have a common att site specificity, but are distinct in their enhancer spe

96 erred strategies for the expansion of target site specificity by EcoRV.

97 suggesting that, during the evolution of new site specificity by integrases, tropism toward a conserv

98 On this basis, we aimed to determine the site specificity by studying differences in modulation b

99 n the 5' to 3' scanning ability and cleavage site specificity characteristic of full-length RNase E a

100 For the degree of site specificity commonly seen, regulators involved in t

101 enzyme did not exhibit altered DNA cleavage site specificity compared to the wild-type enzyme, it di

102 3 loop, where PGT, 2G12, V3, and CD4 binding site specificities competed.

103 pMT1parS site specificity could be altered to that of either P1 o

104 The site specificity differences between I-CreI and I-MsoI s

105 erential cleavage was not due to a change in site specificity, DNA binding, or religation.

106 tory elements that control the timing, organ-site specificity, extent of gene expression, protein lev

107 un1 MORF2 further interacts with RNA-editing site-specificity factors: ORGANELLE TRANSCRIPT PROCESSIN

108 Its site specificity, favorable kinetics, ease of use, and c

109 The cleavage-site specificities for many proteases are not well under

110 OBEC3F and APOBEC3G that confer their target site specificity for cytidine deamination.

111 A3G has a nucleotide target site specificity for cytosine dinucleotides, though only

112 ally demonstrate the importance of catalytic site specificity for directing protein kinase signal tra

113 Tetrahymena activities is their acetylation site specificity for lysines 5, 8, 12, and 16 of H4 and

114 Site specificity for phosphorylation of four PDKs with u

115 for the rapid (2 h) identification of target-site specificity for protein-nucleic acid interactions a

116 e for histone methylation in determining the site specificity for recruitment.

117 peptide arrays to define the phosphorylation site specificity for the majority of STE20 kinases and c

118 dentify a sequence motif underlying cleavage site specificity for the rhomboid proteases.

119 The 3 oldest lineages show target site specificity for unique locations in the genome and

120 raries, offers very high transcription start site specificity, generates accurate and reproducible pr

121 to determine intrinsic features of substrate site specificity have proven challenging.

122 With respect to both substrate and ATP-site specificity, highest similarity is observed between

123 ns and T to A transversions with significant site-specificities, i.e., adjacent to T bases at the 3'-

124 DDR protein, our study aids in understanding site specificity in both generating and interpreting chr

125 olution elucidates the (DMA-135)-RNA binding site specificity in cells and provides insights into the

126 that can be liganded by SuTEx fragments with site specificity in lysates and live cells to disrupt pr

127 strate are consistent with the observed high site specificity in spite of sequence tolerance, while t

128 sively in Atf1, and Pcr1 confers DNA-binding site specificity in vivo.

129 understanding of the basis for the observed site-specificity in cancer development.

130 c of eukaryotic viruses, which showed little site-specificity in colonization.

131 w that the SLFN14 endoribonuclease has broad site-specificity in the absence of modified native tRNA,

132 re of diazonium salt-led to a high degree of site-specificity in the conjugation reaction, even in th

133 ed to such applications because of their low site specificity, in vitro activity, and exceptionally b

134 We find that target site specificity is achieved after DNA binding, which oc

135 ctural analysis defines one pathway by which site specificity is augmented for a homing endonuclease.

136 indings further suggest that phosphorylation site specificity is both necessary and sufficient to med

137 nt, but many questions remain on how binding site specificity is governed.

138 The mechanism that determines target site specificity is not well understood.

139 However, the 9NL27 site specificity is relaxed for some substrate sequences

140 residues lowers the Cu-peptide affinity, but site specificity is retained.

141 Active site specificity loop 309-317, which is disordered in su

142 rate is accompanied by closure of the active site specificity loop, whereas the steroid substrate doe

143 to nascent transcripts on chromatin, but its site-specificity mechanism is mostly unknown.

144 These data support a site specificity model of LCR HS-globin gene interaction

145 lat molecules often lack the protein binding site specificity needed to be an effective drug.

146 However, Tnt1 showed neither unique target site specificity nor strong insertion hot spots, althoug

147 ing parameters can determine in vivo binding site specificity observed for multiprotein signaling com

148 le-helical sequences to compare the cleavage-site specificities of 10 MMPs.

149 Furthermore, the contrasting cleavage site specificities of BACE were not simply due to differ

150 These results highlight the substrate and site specificities of HATs in cells, demonstrate the dis

151 structural modules may explain the different site specificities of the eukaryotic type I topoisomeras

152 The site specificity of 9NL27 may be restored by expanding i

153 fer-based oligonucleotide assay to probe the site specificity of A3G.

154 The cleavage site specificity of activated MPP depends more on the le

155 We show that the DNA-binding site specificity of Aiptasia NF-kappaB is similar to NF-

156 bility, applicability to in vivo studies, or site specificity of amino acid incorporation.

157 r its tropism, but is limited by the lack of site specificity of available conjugation strategies.

158 Examination of the active site specificity of C1r using phage library technology r

159 bsence of RRM3, neither the level nor target-site specificity of cDNA integration was altered.

160 poles where, in B.subtilis, it controls the site specificity of cell division.

161 c, beta5c, and beta2i, to compare the active-site specificity of clinical proteasome inhibitors, and

162 e characterized and clearly demonstrated the site specificity of degradation of the gelator by enzyme

163 mplify how oncogenic signaling regulates the site specificity of DNA demethylation to promote tumorig

164 We demonstrate phospho-site specificity of each antibody in a variety of cultur

165 external guide sequences help determine the site specificity of editing.

166 Our results demonstrate novel site specificity of effects of PKC phosphorylation on cT

167 an together account for the nonlinearity and site specificity of formaldehyde-induced nasal SCC in ra

168 This high site specificity of HEs makes them attractive reagents f

169 tion results in a commensurate change in the site specificity of hypermutation in H chain loci, and t

170 Here, we report that active site specificity of inhibitors can also be tuned by the

171 It is widely believed that active site specificity of inhibitors is determined primarily b

172 The site specificity of IS492 insertion was examined in P. a

173 H3 methylation, we studied the kinetics and site specificity of its modification by CARM1.

174 However, the links between site specificity of Keap1 modification by electrophiles

175 method to establish the initial kinetics and site specificity of light-stimulated rhodopsin phosphory

176 The catalytic site specificity of malarial ADAs permits methylthiocofo

177 provides potentially new means of improving site specificity of MS analysis and new means of assigni

178 sequences but has little or no effect on the site specificity of Msx2 binding.

179 ed sensitivity by exploiting the element and site specificity of near-edge soft X-ray absorption spec

180 in rescue mice to mediate sperm binding, the site specificity of O-glycosylation in both native mZP3

181 Kinetic analysis of the site specificity of p300 autoacetylation reveals a class

182 d p38 phosphorylate the same substrates, the site specificity of phosphorylation can differ as shown

183 in vitro by monitoring the stoichiometry and site specificity of phosphorylation using a combination

184 To demonstrate the site specificity of PKA phosphorylation and its effect o

185 hile revealing a previously unknown cleavage site specificity of Prep when processing certain multi-p

186 scribe a method for determining the cleavage site specificity of proteolytic enzymes that involves po

187 plex virus type 1 and type 2 is the anatomic site specificity of recurrence.

188 radiol dioxygenases provides a rationale for site specificity of ring cleavage.

189 icult to reconcile with the relaxed cleavage site specificity of RNase E, the endonuclease most impor

190 In the present report, the site specificity of the cPAG with respect to the control

191 The magnitude and site specificity of the dopaminergic response may depend

192 ly much higher doses, further confirming the site specificity of the effects.

193 ture, and the length measurements proved the site specificity of the protein in the complex.

194 This step could be crucial for the site specificity of the recombination event that leads t

195 In Saccharomyces cerevisiae, the target site specificity of the retrotransposon Ty1 appears to i

196 We also compare the site specificity of the three major transposons used in

197 a SCN-H-O-Al complex, suggesting the surface site specificity of these experiments.

198 irement for NK cell activity may explain the site specificity of these parasites.

199 that using Mn(2+) as a cofactor, processing site specificity of these substrates was also maintained

200 Thus, the active site specificity of these two protein phosphatases is de

201 ptor positions and explains the unusual dual site specificity of this kinase.

202 The high site specificity of this lateral transfer reaction, term

203 We examined the site specificity of three sigma factors (RpoD/sigma(70),

204 proposed to limit the S1' substrate binding site specificity of thrombin to small polar P1' residues

205 This suggests that the differences in DNA site specificity of topoisomerase II poisoning by XK469

206 The mechanism behind the site specificity of trigeminal nerve injury is still unc

207 The site specificity of TTLL enzymes and their biochemical i

208 is had no effect on incidence, frequency, or site specificity of tumors initiated by the Apc mutation

209 All the site specificity of V(D)J recombination, including the 1

210 Site specificity of VT induction underscores the role of

211 efficiency of cleavage at the desired target site, specificity of cleavage (i.e. rate of cleavage at

212 Encouraged by this finding, the site- specificity of NBD-Bu has been explored through a

213 s has proven to be strongly dependent on the site-specificity of chiral molecules on plasmonic nanopa

214 tal arches, which may be responsible for the site-specificity of dental erosion, and that pellicle do

215 for Na+, indicating that the changes in S1' site-specificity of K60fA thrombin did not result from a

216 We examined the site-specificity of T cell receptor (TCR) repertoires ac

217 To address the site-specificity of the C=O stretches near 1800 cm(-1),

218 In this study the site-specificity of the mature cross-links in human bone

219 his methodology was applied to uncover novel site-specificity of the O-linked glycosylated OmpA/MotB

220 to improve the efficiency and to modify the site-specificity of these enzymes.

221 leolytic cleavages without altering cleavage site specificity or interacting detectably with substrat

222 tivity but did not alter autophosphorylation site specificity or sites of phosphorylation in a robust

223 anded and diversified with demonstrable body site specificity (P < 0.001, R(2) = 0.189) but without d

224 re with surprisingly weak interaction and no site specificity (partition constant, Kp approximately 3

225 for describing transcription factor binding site specificity patterns.

226 pproach proteomic identification of cleavage site specificity (PICS), we identified 286 peptidic clea

227 main of APOBEC3F, exhibits a distinct target site specificity (preferring Y-C dinucleotides) in order

228 ers with functionalized surface ligands with site specificity, putting new ligands in specific positi

229 ication techniques, in particular, achieving site-specificity, reaction efficiency and versatility.

230 h it recognizes its RNA targets and achieves site specificity remain elusive.

231 -affinity binding of RNA, but achieving high site specificity remains a challenging objective.

232 agents with the intent of improving reaction site specificity/reproducibility and ultimately reducing

233 ] conformational specificity and CD4-binding site specificity, respectively) that recapitulate 95% of

234 ion has limited applicability due to lack of site-specificity resulting in the heterogeneity of conju

235 nd AG-to-AA mutations, it had a novel target site specificity, resulting in introduction of GC-to-AC

236 ) side chain in catalysis, inhibition, and P-site specificity, Ser(53) was replaced with threonine, g

237 e not accurate in depicting the true binding site specificities, since these methods rely upon the as

238 s and antibodies suffer limitations, such as site-specificity, stoichiometry and hydrolytic instabili

239 ly expressed and share very similar cleavage site specificities, suggesting that they may be function

240 effect on activity but no effect on cleavage site specificity, suggesting that CSP41 has no sequence

241 This study explores the site specificity (sulfur vs the Fe-Fe bond) of oxygenati

242 material for gas pollutants, with adsorption site specificity taken into account.

243 ansgene appeared to exhibit primary sequence site specificity, targeting a gGATcc-like motif.

244 hat plants possess a protease with substrate-site specificity that is functionally equivalent to cert

245 TOP-53 exhibits a DNA cleavage site specificity that is identical to that of etoposide.

246 To test site specificity, the effect of previous IS was examined

247 However, this mutation did affect the P-site specificity; the glycine mutant can more readily ph

248 Because AID has no known target-site specificity, there have been efforts to identify no

249 a substantial element of antibody combining site specificity, there is scant evidence for V(H) gene

250 ial OSTs follow their own rules for acceptor-site specificity, thereby expanding the glycoengineering

251 gn to engineer HE variants with novel target site specificities to facilitate genome engineering.

252 roximately 20 bp) DNA target sites with high site specificity to catalyze the lateral transfer of par

253 er with local tertiary structure, can confer site specificity to cleavage of cellular and viral subst

254 The findings suggest the possibility of site specificity to environmental influences, as sites i

255 We show that the free PSD binds with site specificity to MARCKS, suggesting that long-range i

256 Notably, the reaction exhibits remarkable site-specificity to cleave peptide bonds at the C-termin

257 nd yet all enzymes display similar catalytic site specificity toward phospholipids with different pol

258 disadvantageous and that the relaxed active-site specificity toward the G-dTTP mispair may be associ

259 pipi*, S2) is investigated with element- and site-specificity using broadband soft X-ray pulses produ

260 NSC 339004), were compared for DNA cleavage site specificity, using purified human topoisomerase IIa

261 ubunit 3G, and speculate about the basis for site specificity vis-a-vis generalized mutagenesis.

262 Validation of probe targets and "G" and "H" site specificity was carried out using a series of compe

263 Low integration site specificity was discerned.

264 3 of HCV were identified, and their cleavage site specificity was investigated.

265 Site specificity was then restored by linking programmab

266 a general strategy to engineer new HE target site specificities, we used RosettaDesign (RD) to genera

267 triplets for maximal activity and 5' splice site specificity, we determined that these elements bind

268 To gain further insight into K-site specificity, we examined the reactions of dansyl-ep

269 accumulation in gene-poor regions and target site specificities were also identified.

270 ally, structural characteristics and binding-site specificity were examined using ion mobility mass s

271 mutations occurring at T bases with the same site-specificities were more prevalent after PUVA-II tre

272 A wide range of rates and site-specificities were observed by these enzyme forms s

273 ne resulted in a relaxation of recombination site specificity, whereas a combination of positive and

274 hts differences and similarities in cleavage site specificities which could be leveraged to develop s

275 his set to 89 cases of independently evolved site specificity, which showed strong bias for the tmRNA

276 that was predicted to display altered target site specificity, while maintaining wild-type binding af

277 endonucleases undergo changes in recognition site specificity will facilitate efforts to engineer pro

278 Ciprofloxacin shows a variation in its site specificity with an apparent preference for G bases

279 We suggest that cleavage site specificity with short DNA duplexes is determined b

280 signal, at WNT16, displayed remarkable bone-site-specificity with no association with hip fractures.

281 eine-substituted peptide in order to develop site specificity within a designed system.

282 Site specificity within the rSC was demonstrated by targ