ライフサイエンスコーパス: site-directed

1 Site-directed acidification of the basic residues within

2 ding positions of Klebsiella oxytoca NasR to site-directed alanine substitutions and measured RNA-bin

3 In this study, we systematically introduced site-directed alterations in individual phosphorylation

4 al structure analysis revealed a dual active site-directed and allosteric inhibition mode of this com

5 Site-directed biopsies revealed enriched MIF and VEGF at

6 led analyses of the PpiD-SecY interaction by site-directed cross-linking revealed that PpiD and the e

7 (SRP), as indicated by in vivo and in vitro site-directed cross-linking.

8 By introducing site-directed Cys residues in bacterial iron transporter

9 -C(3) N(4) ) and carbon cloth (CC), enabling site-directed dendrite-free Li plating.

10 which could offer a simplified solution for site-directed display of small molecule probes and diagn

11 Combined with binding experiments with site-directed ETS1 mutants, the molecular dynamics data

12 complementary polyethylene glycol chain in a site-directed fashion.

13 tif in activated conformation of KvAP-VSD by site-directed fluorescence approaches, using the environ

14 We show that it uses a pac site-directed headful packaging mechanism that results i

15 Site directed incorporation of Cu2+-chelated to a ligand

16 n of the substrate-binding site by an active site-directed inhibitor.

17 We developed a transgenic model using site-directed insertion of the variable domain of a path

18 of this technique for development of active site-directed ligand evolution of peptides, where the nc

19 en the most conserved MMP pair via an active site-directed mechanism of inhibition reported here supp

20 ynamics of biomolecules without the need for site-directed modifications or bio-orthogonal labels.

21 Using site directed mutagenesis and a site-specific phospho-an

22 Site directed mutagenesis of lysine 68 to glutamine (K68

23 Further, site directed mutagenesis of the miR-134 binding site in

24 Site directed mutagenesis results show that residues in

25 well as the AdoMet-binding motif (FXGXG) by site-directed mutagenesis abolished the catalytic activi

26 hese 2 intermediates and iterative rounds of site-directed mutagenesis allowed us to identify 4 of 41

27 etic data and a catalytic model supported by site-directed mutagenesis allows full comparison with di

28 Site-directed mutagenesis allows the generation of novel

29 Kinetic, molecular docking, and site-directed mutagenesis analyses confirmed these compo

30 We have carried out an extensive site-directed mutagenesis analysis of the CD16A receptor

31 Using site-directed mutagenesis and a specific in vitro transc

32 Using domain truncations, site-directed mutagenesis and an unbiased forward geneti

33 Furthermore, transcriptional start analysis, site-directed mutagenesis and bacterial two-hybrid inter

34 scale quantum and classical simulations with site-directed mutagenesis and biophysical experiments, w

35 op is relocated by pressure was validated by site-directed mutagenesis and by inhibition by small pep

36 Results from site-directed mutagenesis and competitive ligand-binding

37 y activity-based protein profiling utilizing site-directed mutagenesis and computational modeling.

38 monstrate that ChABC can be stabilized using site-directed mutagenesis and covalent modification with

39 modeling with biochemical validation through site-directed mutagenesis and enzyme activity assays, we

40 MD) simulations were performed together with site-directed mutagenesis and enzyme kinetic studies to

41 The prediction was validated by site-directed mutagenesis and evaluation of the relative

42 amino acids, which can be altered readily by site-directed mutagenesis and expressed in vitro and in

43 Structural analysis combined with site-directed mutagenesis and functional studies inform

44 utative binding sites of C3G and GSH through site-directed mutagenesis and functional studies.

45 Site-directed mutagenesis and gene rescue studies show t

46 rs in the structure, and verified them using site-directed mutagenesis and in vitro hormone-productio

47 role of these putative glycosylation sites, site-directed mutagenesis and lectin affinity purificati

48 Site-directed mutagenesis and mass spectrometry experime

49 controlled proteolysis, gel electrophoresis, site-directed mutagenesis and microsecond MD simulations

50 brafish and human MR coupled with reciprocal site-directed mutagenesis and molecular dynamic (MD) sim

51 ubstrate and product complexes together with site-directed mutagenesis and molecular dynamics simulat

52 tobacco (Nicotiana tabacum L.) using CRISPR site-directed mutagenesis and overexpression assays.

53 Here we used site-directed mutagenesis and patch-clamp recordings to

54 We used site-directed mutagenesis and patch-clamp recordings to

55 Using site-directed mutagenesis and phosphorylation analysis o

56 onas reinhardtii (CrHydA1) we have conducted site-directed mutagenesis and protein film electrochemis

57 We used site-directed mutagenesis and single-cell patch-clamp to

58 We used site-directed mutagenesis and single-cell patch-clamp to

59 Here, using site-directed mutagenesis and stopped-flow kinetics, we

60 Site-directed mutagenesis and structural modeling analys

61 By site-directed mutagenesis and structure-guided analyses,

62 a molecular mechanism that was supported by site-directed mutagenesis and targeted molecular dynamic

63 d mitochondrial function, as demonstrated by site-directed mutagenesis and use of STAT3 knockout and

64 aging hippocampus, together with an in vitro site-directed mutagenesis approach, we identify loss of

65 rucial for stabilizing mutp53(R175H) using a site-directed mutagenesis approach.

66 To overcome the limitations of existing site-directed mutagenesis approaches, we explored in viv

67 s, size exclusion chromatography, as well as site-directed mutagenesis approaches, we show that the A

68 multiple DNA fragments and did simultaneous site-directed mutagenesis at multiple sites.

69 y determined by cryo-electron microscopy and site-directed mutagenesis at the minus beta2 side conver

70 tigenic analysis of Der p 2 was performed by site-directed mutagenesis based on the x-ray crystal str

71 Site-directed mutagenesis confirmed the essential role t

72 hese mutations into a wt infectious clone by site-directed mutagenesis considerably reduced replicati

73 We used site-directed mutagenesis coupled with direct measures o

74 xtensive structure-activity relationship and site-directed mutagenesis data facilitates the predictio

75 Experimental validation is by in-vitro, site-directed mutagenesis data found in literature.

76 Site-directed mutagenesis data provides an invaluable co

77 Site-directed mutagenesis demonstrated that substitution

78 Furthermore, site-directed mutagenesis determined that C61, C69, C137

79 ne details of IDP-SNP affinity profiles, and site-directed mutagenesis effects with a spatial resolut

80 his study, structural analyses combined with site-directed mutagenesis efficiently improved the funct

81 tential energies and molecular dynamics, and site-directed mutagenesis establish the mechanism of thi

82 ing and antigenic properties, we carried out site-directed mutagenesis experiments at the single-amin

83 potential interaction sites for PI(4,5)P(2) Site-directed mutagenesis experiments confirmed that the

84 four cysteines within the mature peptide and site-directed mutagenesis experiments demonstrated that

85 the catalysis are discussed and compared to site-directed mutagenesis experiments.

86 In silico modeling and site-directed mutagenesis identified charged residues at

87 Systematic site-directed mutagenesis identified the core amino acid

88 Molecular modeling and site-directed mutagenesis implicate several residues aro

89 Combining brain slice studies and site-directed mutagenesis in HEK293T cells, we found tha

90 study, we targeted individual amino acids by site-directed mutagenesis in the [FeFe]-hydrogenase CpI

91 muscle dysfunctions related to mutations or site-directed mutagenesis in titin that alter the I-band

92 estabilization of the coiled-coil domains by site-directed mutagenesis increases the effective diffus

93 Site-directed mutagenesis indicates that tyrosine 645 in

94 -biased multiplex mutagenesis, over existing site-directed mutagenesis methods such as recombineering

95 Site-directed mutagenesis near either FAD produced alter

96 Molecular modeling and site-directed mutagenesis of ARH3 revealed that numerous

97 Site-directed mutagenesis of both cofilin and actin reve

98 n of the redox state of FAD4 or PRXQ through site-directed mutagenesis of conserved cysteine residues

99 resonance (DEER) with functional assays and site-directed mutagenesis of conserved residues to illum

100 sible spectroscopy, heme quantification, and site-directed mutagenesis of histidine residues, we demo

101 re analysis of PcUP1 with bound ligands, and site-directed mutagenesis of key residues provide additi

102 Site-directed mutagenesis of lsBSH demonstrated that Cys

103 Site-directed mutagenesis of NEM1 and SPO7, coupled with

104 stal structure of the AT1R was used to guide site-directed mutagenesis of outward-facing hydrophobic

105 Site-directed mutagenesis of phosphorylated residues rev

106 structure and function of CHT7, we performed site-directed mutagenesis of previously identified phosp

107 Site-directed mutagenesis of PxaTPS8 revealed several ca

108 Site-directed mutagenesis of RadH was used to identify c

109 Site-directed mutagenesis of RCAR1 showed that its tyros

110 Using site-directed mutagenesis of residues in both proteins,

111 Site-directed mutagenesis of residues near the predicted

112 lico findings is validated experimentally by site-directed mutagenesis of the key CcmK2 residue Serin

113 Site-directed mutagenesis of the phytaspase cleavage sit

114 Site-directed mutagenesis of the Sp1-binding site within

115 By exploiting site-directed mutagenesis of the specific amino acids we

116 affect the activity of G. lovleyi NrfA, and site-directed mutagenesis of this arginine reduced enzym

117 Site-directed mutagenesis of Thr-48 to Ala (T48A) to pre

118 riments, the use of amino acid blockers, and site-directed mutagenesis of UCP1, we propose a mechanis

119 Here, we performed site-directed mutagenesis on five envelope (E) protein a

120 In this study, we made site-directed mutagenesis on the surface-exposed hydroph

121 ties of truncated MT2 that were generated by site-directed mutagenesis or Gibson assembly master mix,

122 Past studies, based on site-directed mutagenesis or solvatochromism of the isol

123 oteins by top-down MS, NMR spectroscopy, and site-directed mutagenesis revealed specific and well-con

124 Molecular modeling/simulation coupled with site-directed mutagenesis revealed that Asp(147) or Asn(

125 Site-directed mutagenesis revealed that GRK2 Ser-685 pho

126 Site-directed mutagenesis revealed that Leu(46) and Phe(

127 Site-directed mutagenesis showed that Ser-491 is essenti

128 Site-directed mutagenesis showed that the relevant sites

129 Site-directed mutagenesis studies revealed four substitu

130 Mossbauer spectroscopy measurements and the site-directed mutagenesis studies show that the red Fur

131 Extensive site-directed mutagenesis studies supported the importan

132 Site-directed mutagenesis studies were consistent with b

133 In this study, using computational and site-directed mutagenesis studies, we demonstrate the pr

134 ombined in silico hA(3)AR-homology model and site-directed mutagenesis study was performed to demonst

135 Site-directed mutagenesis supports a model in which aden

136 Site-directed mutagenesis targeting these tyrosine resid

137 cDNAs were constructed by site-directed mutagenesis that encode PORB mutant protei

138 Furthermore, we show by site-directed mutagenesis that tyrosine (Y382-384) withi

139 Site-directed mutagenesis then revealed that a previousl

140 n of CD2AP, and phospho-CD2AP antibodies and site-directed mutagenesis to define the specific phospho

141 GAT1 in MGAT4D-L transfectants, we performed site-directed mutagenesis to determine requirements for

142 m different biological kingdoms and employed site-directed mutagenesis to elucidate the molecular bas

143 searches for candidate exosites, we utilized site-directed mutagenesis to identify a new exosite in c

144 6 ECR with high-nanomolar affinity, and used site-directed mutagenesis to identify a patch of conserv

145 In the current study, we use site-directed mutagenesis to identify K(100) and K(188)

146 evan-type fructooligosaccharide and utilized site-directed mutagenesis to identify residues involved

147 Using site-directed mutagenesis to test multiple potential sit

148 We used X-ray crystallography and site-directed mutagenesis to unravel the molecular and s

149 ceptor, and validated this interface through site-directed mutagenesis together with functional and c

150 he effect on STAT4 function was evaluated by site-directed mutagenesis using a lymphoblastoid B cell

151 Site-directed mutagenesis was performed by partial gene

152 Site-directed mutagenesis was subsequently used to ident

153 Site-directed mutagenesis was used to create orthogonal

154 -Golgi enzyme alpha2,6-sialyltransferase and site-directed mutagenesis was used to exchange individua

155 Site-directed mutagenesis was used to investigate the re

156 Site-directed mutagenesis was utilized to assess the rol

157 test the transition state model, we combined site-directed mutagenesis with kinetic experiments, yiel

158 mic interactions to validation using data on site-directed mutagenesis' effect on capsid assembly.

159 nition and hydrolysis using a combination of site-directed mutagenesis, activity measurements using s

160 Here, employing site-directed mutagenesis, allelic exchange, quantitativ

161 In this study, using purified proteins and site-directed mutagenesis, along with fluorescence polar

162 Through NMR studies, site-directed mutagenesis, and advanced molecular dynami

163 s with tryptophan fluorescence spectroscopy, site-directed mutagenesis, and antibiotic susceptibility

164 ormed with the help of isotopic labeling and site-directed mutagenesis, and complemented by integrati

165 nity assays, sugar binding pocket deletions, site-directed mutagenesis, and construction of chimeric

166 have used the chemical modification of Trp9, site-directed mutagenesis, and crystallographic and comp

167 homology modeling, coevolutionary analyses, site-directed mutagenesis, and electrophysiology to exam

168 f homology protein modeling, ligand docking, site-directed mutagenesis, and electrophysiology, we sho

169 particularly strong interactions, performed site-directed mutagenesis, and expressed the mutants in

170 lec-7, we carried out in silico analysis and site-directed mutagenesis, and found a new sialic acid-b

171 e mapping was performed by pepscan analysis, site-directed mutagenesis, and hydrogen/deuterium exchan

172 from crystal structures, molecular docking, site-directed mutagenesis, and kinetic and thermodynamic

173 tion-quantitative polymerase chain reaction, site-directed mutagenesis, and micrococcal nuclease assa

174 mbination of NMR, fluorescence spectroscopy, site-directed mutagenesis, and thermodynamics to elucida

175 vity relationships, using electrophysiology, site-directed mutagenesis, and voltage-clamp fluorometry

176 Remarkably, biophysical approaches, site-directed mutagenesis, and X-ray crystallography unc

177 Through site-directed mutagenesis, based on a modeled structure,

178 In this study, using site-directed mutagenesis, biochemical and biophysical t

179 By combining site-directed mutagenesis, biochemical assays, and spect

180 We performed site-directed mutagenesis, cellular localization experim

181 Here, using several approaches, including site-directed mutagenesis, Click O-GlcNAc labeling, immu

182 rroborated with X-ray co-crystallography and site-directed mutagenesis, collectively reveal the struc

183 n CYP2B enzymes using X-ray crystallography, site-directed mutagenesis, deuterium-exchange MS, isothe

184 In this study, using COS-7 cells, site-directed mutagenesis, electrophysiological measurem

185 Using a combination of site-directed mutagenesis, electrophysiology, and modeli

186 oscopy, cryo-EM, and CD analyses, along with site-directed mutagenesis, enzymatic assays, and molecul

187 Here, using recombinant protein expression, site-directed mutagenesis, fluorimetric and biochemical

188 Here, we used site-directed mutagenesis, glutathione S-transferase pul

189 Here, using site-directed mutagenesis, GTP hydrolysis assays, coimmu

190 ere, using an array of techniques, including site-directed mutagenesis, immunoblotting, FRET, and pro

191 Combined with in silico modeling, site-directed mutagenesis, inhibition experiments and ce

192 study, using statistical coupling analysis, site-directed mutagenesis, isothermal calorimetry, small

193 Using site-directed mutagenesis, kinetic assays, and quantitat

194 ue 17 from the N terminus from Thr to Asn by site-directed mutagenesis, making it constitutively inac

195 Here, we combine electrochemistry, site-directed mutagenesis, molecular dynamics and quantu

196 Here, using site-directed mutagenesis, molecular modeling, and in ce

197 crystallography, in vitro enzyme assays and site-directed mutagenesis, of the bacillaene synthase de

198 Using site-directed mutagenesis, optical trap-based force spec

199 ge of in vitro and rat in situ preparations, site-directed mutagenesis, patch-clamp, nerve recordings

200 n glycosyltransferase structural biology and site-directed mutagenesis, pathway engineering, and deox

201 Using site-directed mutagenesis, purified recombinant proteins

202 t and its implications for heme transfer via site-directed mutagenesis, resonance Raman (RR), hydroge

203 mate at 1.94 angstrom resolution, along with site-directed mutagenesis, revealed how the active site

204 C-infected patients (n = 168), together with site-directed mutagenesis, revealed Nef position 9 as a

205 ogical and biochemical approaches, including site-directed mutagenesis, reverse genetics-based virus

206 ere, using several approaches, including MS, site-directed mutagenesis, siRNA-mediated gene silencing

207 T TtCE15A and variants in which we, by using site-directed mutagenesis, substituted residues suggeste

208 Using site-directed mutagenesis, surface plasmon resonance, an

209 Using site-directed mutagenesis, we confirmed that the predict

210 Using truncation mutants and site-directed mutagenesis, we define the inhibitory face

211 Using site-directed mutagenesis, we demonstrate that the M159I

212 Through site-directed mutagenesis, we demonstrate that these res

213 Using site-directed mutagenesis, we demonstrated that the cons

214 Using site-directed mutagenesis, we determined that glycosylat

215 nce resonance energy transfer technique, and site-directed mutagenesis, we examined the domains invol

216 with purified and recombinant proteins, and site-directed mutagenesis, we examined the role of a put

217 Using site-directed mutagenesis, we found that mutations predi

218 Using site-directed mutagenesis, we further defined this addit

219 Using site-directed mutagenesis, we generated FOXM1 mutant pro

220 gh a combination of in silico prediction and site-directed mutagenesis, we have mapped an exosite to

221 sing homology modeling of ENaC structure and site-directed mutagenesis, we identified a cleft region

222 Here, by using site-directed mutagenesis, we identified Arg-8 of subuni

223 Using in silico docking and site-directed mutagenesis, we identified Asp(230) in the

224 Using site-directed mutagenesis, we identified serine-465 as t

225 In addition, using site-directed mutagenesis, we identify those amino acids

226 Through extensive site-directed mutagenesis, we map phosphorylation to the

227 Using site-directed mutagenesis, we mutated the specific sites

228 in interaction and kinetic assays as well as site-directed mutagenesis, we provide further insight in

229 using protein-lipid co-flotation assays and site-directed mutagenesis, we report that the N-region o

230 ioinformatics-based approaches combined with site-directed mutagenesis, we show here that SLFN11 is p

231 nalyses, molecular dynamics simulations, and site-directed mutagenesis, we show that 8-NBD-cGMP inhib

232 Here, using site-directed mutagenesis, we show that Asn-26 in the mo

233 Through site-directed mutagenesis, we show that three amino acid

234 Using site-directed mutagenesis, we validated those surfaces i

235 ructure, we identified catalytic residues by site-directed mutagenesis.

236 The two activities can be separated by site-directed mutagenesis.

237 ructure-function relationship of (Pl)EctA by site-directed mutagenesis.

238 o genetically engineer these regions through site-directed mutagenesis.

239 ulate the binding interface were verified by site-directed mutagenesis.

240 he channel outer vestibule experimentally by site-directed mutagenesis.

241 ration transfer difference NMR (STD-NMR) and site-directed mutagenesis.

242 was substituted by a cysteine codon through site-directed mutagenesis.

243 ons such as targeted genome modification and site-directed mutagenesis.

244 erminants on both allergens was performed by site-directed mutagenesis.

245 tion of these binding sites was confirmed by site-directed mutagenesis.

246 ding site in the ion pore that we confirm by site-directed mutagenesis.

247 genotype 2a-derived HCV clone (Jc1Gluc2A) by site-directed mutagenesis.

248 epair in E coli supports efficient multiplex site-directed mutagenesis.

249 These determinants were resolved further by site-directed mutagenesis.

250 Some of these residues were probed using site-directed mutagenesis.

251 on function and validated six of these using site-directed mutagenesis.

252 ely disassociated to varying extents through site-directed mutagenesis.

253 ed amino acids critical for Opi3 activity by site-directed mutagenesis.

254 lations, kinetic studies, and the results of site-directed mutagenesis.

255 Transfection of a NME1 site-directed mutant lacking histidine protein kinase ac

256 al and genetic data could be used to predict site-directed mutant phenotypes, which may broaden the c

257 f CbiK(P) was studied by constructing eleven site-directed mutants and determining their chelatase ac

258 rystal structure and biochemical analysis of site-directed mutants identifies a catalytic histidine a

259 Analysis of additional site-directed mutants in the background of the D506N:D57

260 However, catalytically inactive site-directed mutants of BoGH9 fail to complement the de

261 Functional characterization of site-directed mutants targeting the substrate- and NAD(H

262 Using defined synthetic acceptors and site-directed mutants to assign the activities of the Wb

263 C50 and C61 of CpeB was also explored using site-directed mutants, revealing that linkage at the A r

264 .99- angstrom resolution and assayed several site-directed mutants.

265 plementation studies using LtgG containing a site directed mutation in aspartate 343, confirmed the e

266 by a wealth of functional data generated by site-directed mutation experiments.

267 molecular agonist was initially indicated by site-directed mutation studies and activating peptides d

268 tructs encoding RBD1 protein truncations and site-directed mutations demonstrated that the TMH domain

269 esidue in the beta3 S-x(3)-A-x(3)-I motif by site-directed mutations is sufficient to induce alphavbe

270 otrimer for chemotaxis was demonstrated with site-directed mutations of key amino acid residues in C.

271 Analysis of transposase proteins containing site-directed mutations revealed the importance of the c

272 ermined the efficiency of DeGeRing to induce site-directed mutations with and without a phenotype in

273 n combination with a series of deletions and site-directed mutations, we determined that the first 22

274 h Thr78-to-Ala (T78A) or Thr78-to-Ser (T78S) site-directed mutations.

275 pound that is structurally similar to active-site directed NAMPT inhibitors and blocks binding of the

276 he viraemic timepoint were sequenced using a site-directed next-generation sequencing assay for the r

277 Site-directed nucleases (SDNs) used for targeted genome

278 on ethanol consumption was evaluated through site-directed pharmacology.

279 Furthermore, using an active-site-directed photoprobe with double cross-linking moiet

280 rapies have created new opportunities to use site-directed, programmable drug delivery systems to tre

281 ucleosomes and validated these structures by site-directed protein cross-linking and hydroxyl radical

282 nsive cytotoxic chemotherapy with or without site-directed radiotherapy and allogeneic hematopoietic

283 analyses confirmed these compounds as active site-directed reversible inhibitors of mPTPB.

284 Site-directed RNA editing might provide a safer or more

285 Here, we apply the approach of site-directed RNA editing to repair, at the mRNA level,

286 Here, we present the application of a site-directed screening technique based on disulfide tra

287 havior by using atomic-level simulations and site-directed spectroscopy to reveal the effects of phos

288 Site directed spin labeling EPR and DEER (double electro

289 Site-directed spin labeling (SDSL) ESR is a valuable too

290 c resonance (EPR) spectroscopy, coupled with site-directed spin labeling (SDSL), is a useful method f

291 ces 6 and 7 at the cytoplasmic surface using site-directed spin labeling and double electron-electron

292 proach to overcome this problem by utilizing site-directed spin labeling and electron paramagnetic re

293 veraging sparse distance data gathered using site-directed spin labeling and electron paramagnetic re

294 This model is supported by site-directed spin labeling and electron paramagnetic re

295 membrane-reconstituted synaptotagmin 1 using site-directed spin labeling in which we characterize the

296 c resonance spectroscopy in combination with site-directed spin labeling, we show that familial PD-as

297 l dynamics of the C-terminus of EcMscL using site-directed spin labelling electron paramagnetic reson

298 In this study, we used site-directed spin-labeling electron paramagnetic resona

299 1 together with STAM1 activates FAK, we used site-directed spin-labeling EPR spectroscopy-based studi

300 assembled to homogeneity and investigated by site-directed spin-labeling with pulse-dipolar electron-