ライフサイエンスコーパス: site-directed mutagenesis

1 nt to CAT2 promoter activity was analyzed by site directed mutagenesis.

2 The two activities can be separated by site-directed mutagenesis.

3 o genetically engineer these regions through site-directed mutagenesis.

4 ructure-function relationship of (Pl)EctA by site-directed mutagenesis.

5 ulate the binding interface were verified by site-directed mutagenesis.

6 he channel outer vestibule experimentally by site-directed mutagenesis.

7 ration transfer difference NMR (STD-NMR) and site-directed mutagenesis.

8 was substituted by a cysteine codon through site-directed mutagenesis.

9 ons such as targeted genome modification and site-directed mutagenesis.

10 epair in E coli supports efficient multiplex site-directed mutagenesis.

11 erminants on both allergens was performed by site-directed mutagenesis.

12 tion of these binding sites was confirmed by site-directed mutagenesis.

13 ding site in the ion pore that we confirm by site-directed mutagenesis.

14 genotype 2a-derived HCV clone (Jc1Gluc2A) by site-directed mutagenesis.

15 r export signal, and could be inactivated by site-directed mutagenesis.

16 stion with native mass spectrometry (MS) and site-directed mutagenesis.

17 hains, adding a dynamic dimension to protein site-directed mutagenesis.

18 eir catalytic residues were identified using site-directed mutagenesis.

19 validated the predicted interface in vivo by site-directed mutagenesis.

20 These determinants were resolved further by site-directed mutagenesis.

21 Some of these residues were probed using site-directed mutagenesis.

22 on function and validated six of these using site-directed mutagenesis.

23 ely disassociated to varying extents through site-directed mutagenesis.

24 ed amino acids critical for Opi3 activity by site-directed mutagenesis.

25 lations, kinetic studies, and the results of site-directed mutagenesis.

26 ructure, we identified catalytic residues by site-directed mutagenesis.

27 well as the AdoMet-binding motif (FXGXG) by site-directed mutagenesis abolished the catalytic activi

28 nition and hydrolysis using a combination of site-directed mutagenesis, activity measurements using s

29 Here, employing site-directed mutagenesis, allelic exchange, quantitativ

30 hese 2 intermediates and iterative rounds of site-directed mutagenesis allowed us to identify 4 of 41

31 from a genetically encoded peptide scaffold, site-directed mutagenesis allows for rapid generation of

32 etic data and a catalytic model supported by site-directed mutagenesis allows full comparison with di

33 Site-directed mutagenesis allows the generation of novel

34 In this study, using purified proteins and site-directed mutagenesis, along with fluorescence polar

35 Kinetic, molecular docking, and site-directed mutagenesis analyses confirmed these compo

36 We have carried out an extensive site-directed mutagenesis analysis of the CD16A receptor

37 Using site directed mutagenesis and a site-specific phospho-an

38 Using site-directed mutagenesis and a specific in vitro transc

39 Using domain truncations, site-directed mutagenesis and an unbiased forward geneti

40 Furthermore, transcriptional start analysis, site-directed mutagenesis and bacterial two-hybrid inter

41 scale quantum and classical simulations with site-directed mutagenesis and biophysical experiments, w

42 op is relocated by pressure was validated by site-directed mutagenesis and by inhibition by small pep

43 hese residues between E. coli RF1 and RF2 by site-directed mutagenesis and characterized their prefer

44 Results from site-directed mutagenesis and competitive ligand-binding

45 y activity-based protein profiling utilizing site-directed mutagenesis and computational modeling.

46 monstrate that ChABC can be stabilized using site-directed mutagenesis and covalent modification with

47 In this report, combining single site-directed mutagenesis and double mutant analyses, we

48 pore lumen, which could both be confirmed by site-directed mutagenesis and electrophysiology.

49 modeling with biochemical validation through site-directed mutagenesis and enzyme activity assays, we

50 MD) simulations were performed together with site-directed mutagenesis and enzyme kinetic studies to

51 The prediction was validated by site-directed mutagenesis and evaluation of the relative

52 amino acids, which can be altered readily by site-directed mutagenesis and expressed in vitro and in

53 Through site-directed mutagenesis and functional analysis, we fo

54 Structural analysis combined with site-directed mutagenesis and functional studies inform

55 utative binding sites of C3G and GSH through site-directed mutagenesis and functional studies.

56 Site-directed mutagenesis and gene rescue studies show t

57 e nucleus of HTB-9 cells, as demonstrated by site-directed mutagenesis and green fluorescent protein

58 Site-directed mutagenesis and homology models show the i

59 rs in the structure, and verified them using site-directed mutagenesis and in vitro hormone-productio

60 role of these putative glycosylation sites, site-directed mutagenesis and lectin affinity purificati

61 Site-directed mutagenesis and mass spectrometry experime

62 controlled proteolysis, gel electrophoresis, site-directed mutagenesis and microsecond MD simulations

63 brafish and human MR coupled with reciprocal site-directed mutagenesis and molecular dynamic (MD) sim

64 ubstrate and product complexes together with site-directed mutagenesis and molecular dynamics simulat

65 tobacco (Nicotiana tabacum L.) using CRISPR site-directed mutagenesis and overexpression assays.

66 Here we used site-directed mutagenesis and patch-clamp recordings to

67 We used site-directed mutagenesis and patch-clamp recordings to

68 Using site-directed mutagenesis and phosphorylation analysis o

69 onas reinhardtii (CrHydA1) we have conducted site-directed mutagenesis and protein film electrochemis

70 The structure is confirmed by site-directed mutagenesis and provides a molecular frame

71 We used site-directed mutagenesis and single-cell patch-clamp to

72 We used site-directed mutagenesis and single-cell patch-clamp to

73 Here, using site-directed mutagenesis and stopped-flow kinetics, we

74 Site-directed mutagenesis and structural modeling analys

75 By site-directed mutagenesis and structure-guided analyses,

76 a molecular mechanism that was supported by site-directed mutagenesis and targeted molecular dynamic

77 y understood, we used its structure to guide site-directed mutagenesis and to dissect its function.

78 We used site-directed mutagenesis and transient-kinetic approach

79 d mitochondrial function, as demonstrated by site-directed mutagenesis and use of STAT3 knockout and

80 Through NMR studies, site-directed mutagenesis, and advanced molecular dynami

81 s with tryptophan fluorescence spectroscopy, site-directed mutagenesis, and antibiotic susceptibility

82 Through a combination of SPOT peptide array, site-directed mutagenesis, and bio-layer interferometry,

83 ormed with the help of isotopic labeling and site-directed mutagenesis, and complemented by integrati

84 nity assays, sugar binding pocket deletions, site-directed mutagenesis, and construction of chimeric

85 have used the chemical modification of Trp9, site-directed mutagenesis, and crystallographic and comp

86 homology modeling, coevolutionary analyses, site-directed mutagenesis, and electrophysiology to exam

87 f homology protein modeling, ligand docking, site-directed mutagenesis, and electrophysiology, we sho

88 particularly strong interactions, performed site-directed mutagenesis, and expressed the mutants in

89 of methods including chemical modification, site-directed mutagenesis, and fluorescent spectroscopy,

90 lec-7, we carried out in silico analysis and site-directed mutagenesis, and found a new sialic acid-b

91 e mapping was performed by pepscan analysis, site-directed mutagenesis, and hydrogen/deuterium exchan

92 from crystal structures, molecular docking, site-directed mutagenesis, and kinetic and thermodynamic

93 ction, were deduced from crystal structures, site-directed mutagenesis, and kinetic and thermodynamic

94 SAR) of Tg for SPCA1a by in silico modeling, site-directed mutagenesis, and measuring the potency of

95 tion-quantitative polymerase chain reaction, site-directed mutagenesis, and micrococcal nuclease assa

96 mbination of NMR, fluorescence spectroscopy, site-directed mutagenesis, and thermodynamics to elucida

97 vity relationships, using electrophysiology, site-directed mutagenesis, and voltage-clamp fluorometry

98 Remarkably, biophysical approaches, site-directed mutagenesis, and X-ray crystallography unc

99 Using MS analysis, site-directed mutagenesis, and X-ray structural data ana

100 aging hippocampus, together with an in vitro site-directed mutagenesis approach, we identify loss of

101 rucial for stabilizing mutp53(R175H) using a site-directed mutagenesis approach.

102 To overcome the limitations of existing site-directed mutagenesis approaches, we explored in viv

103 s, size exclusion chromatography, as well as site-directed mutagenesis approaches, we show that the A

104 ne chemical labeling, mass spectrometry, and site-directed mutagenesis approaches, we show that thira

105 from a unique cysteine residue introduced by site-directed mutagenesis) as free-radical trapping 'tag

106 , as pinpointed by computational results and site-directed mutagenesis at Ala33.

107 multiple DNA fragments and did simultaneous site-directed mutagenesis at multiple sites.

108 y determined by cryo-electron microscopy and site-directed mutagenesis at the minus beta2 side conver

109 tigenic analysis of Der p 2 was performed by site-directed mutagenesis based on the x-ray crystal str

110 Through site-directed mutagenesis, based on a modeled structure,

111 In this study, using site-directed mutagenesis, biochemical and biophysical t

112 A combination of site-directed mutagenesis, biochemical and electrophysio

113 By combining site-directed mutagenesis, biochemical assays, and spect

114 We performed site-directed mutagenesis, cellular localization experim

115 models, NMR titrations, DNA-binding studies, site-directed mutagenesis, charge distribution, and sequ

116 Here, using several approaches, including site-directed mutagenesis, Click O-GlcNAc labeling, immu

117 rroborated with X-ray co-crystallography and site-directed mutagenesis, collectively reveal the struc

118 KEY MESSAGE: Successful site-directed mutagenesis combined with in silico modeli

119 Truncation mutations and site-directed mutagenesis confirmed that the KH domain i

120 Site-directed mutagenesis confirmed the essential role t

121 The molecular dynamics simulation and site-directed mutagenesis confirmed the important roles

122 Site-directed mutagenesis confirms the observed interact

123 hese mutations into a wt infectious clone by site-directed mutagenesis considerably reduced replicati

124 We used site-directed mutagenesis coupled with direct measures o

125 Using a combination of site-directed mutagenesis, DA-uptake, and cross-linking

126 xtensive structure-activity relationship and site-directed mutagenesis data facilitates the predictio

127 Experimental validation is by in-vitro, site-directed mutagenesis data found in literature.

128 Site-directed mutagenesis data provides an invaluable co

129 Site-directed mutagenesis demonstrated that substitution

130 Furthermore, site-directed mutagenesis determined that C61, C69, C137

131 n CYP2B enzymes using X-ray crystallography, site-directed mutagenesis, deuterium-exchange MS, isothe

132 mic interactions to validation using data on site-directed mutagenesis' effect on capsid assembly.

133 ne details of IDP-SNP affinity profiles, and site-directed mutagenesis effects with a spatial resolut

134 his study, structural analyses combined with site-directed mutagenesis efficiently improved the funct

135 In this study, using COS-7 cells, site-directed mutagenesis, electrophysiological measurem

136 Using a combination of site-directed mutagenesis, electrophysiology, and modeli

137 oscopy, cryo-EM, and CD analyses, along with site-directed mutagenesis, enzymatic assays, and molecul

138 tential energies and molecular dynamics, and site-directed mutagenesis establish the mechanism of thi

139 ing and antigenic properties, we carried out site-directed mutagenesis experiments at the single-amin

140 potential interaction sites for PI(4,5)P(2) Site-directed mutagenesis experiments confirmed that the

141 four cysteines within the mature peptide and site-directed mutagenesis experiments demonstrated that

142 the catalysis are discussed and compared to site-directed mutagenesis experiments.

143 g-range coupling effects and predictions for site-directed mutagenesis experiments.

144 Here, using recombinant protein expression, site-directed mutagenesis, fluorimetric and biochemical

145 Here, we used site-directed mutagenesis, glutathione S-transferase pul

146 Here, using site-directed mutagenesis, GTP hydrolysis assays, coimmu

147 ic pH-driven hENT3 nucleoside transport with site-directed mutagenesis, homology modeling, and [(3)H]

148 In silico modeling and site-directed mutagenesis identified charged residues at

149 ly, and computational analysis together with site-directed mutagenesis identified five basic residues

150 Systematic site-directed mutagenesis identified the core amino acid

151 ere, using an array of techniques, including site-directed mutagenesis, immunoblotting, FRET, and pro

152 Molecular modeling and site-directed mutagenesis implicate several residues aro

153 study, we addressed these questions by using site-directed mutagenesis in combination with enzymatic,

154 Combining brain slice studies and site-directed mutagenesis in HEK293T cells, we found tha

155 study, we targeted individual amino acids by site-directed mutagenesis in the [FeFe]-hydrogenase CpI

156 muscle dysfunctions related to mutations or site-directed mutagenesis in titin that alter the I-band

157 estabilization of the coiled-coil domains by site-directed mutagenesis increases the effective diffus

158 Computational modeling and site-directed mutagenesis indicated that the mode of fat

159 Site-directed mutagenesis indicates that tyrosine 645 in

160 Combined with in silico modeling, site-directed mutagenesis, inhibition experiments and ce

161 Site directed mutagenesis is widely used to understand t

162 study, using statistical coupling analysis, site-directed mutagenesis, isothermal calorimetry, small

163 Using site-directed mutagenesis, kinetic assays, and quantitat

164 By site-directed mutagenesis, L249P was identified as the c

165 ue 17 from the N terminus from Thr to Asn by site-directed mutagenesis, making it constitutively inac

166 -biased multiplex mutagenesis, over existing site-directed mutagenesis methods such as recombineering

167 Compared with alternative site-directed mutagenesis methods, our protocol requires

168 Here, we combine electrochemistry, site-directed mutagenesis, molecular dynamics and quantu

169 Here, using site-directed mutagenesis, molecular modeling, and in ce

170 Site-directed mutagenesis near either FAD produced alter

171 Site directed mutagenesis of lysine 68 to glutamine (K68

172 Further, site directed mutagenesis of the miR-134 binding site in

173 Furthermore, site-directed mutagenesis of a predicted ETS-binding sit

174 Site-directed mutagenesis of any of the two conserved ca

175 Molecular modeling and site-directed mutagenesis of ARH3 revealed that numerous

176 Site-directed mutagenesis of both cofilin and actin reve

177 n of the redox state of FAD4 or PRXQ through site-directed mutagenesis of conserved cysteine residues

178 resonance (DEER) with functional assays and site-directed mutagenesis of conserved residues to illum

179 Site-directed mutagenesis of GluN1-Glu781 reduced the po

180 sible spectroscopy, heme quantification, and site-directed mutagenesis of histidine residues, we demo

181 re analysis of PcUP1 with bound ligands, and site-directed mutagenesis of key residues provide additi

182 Site-directed mutagenesis of lsBSH demonstrated that Cys

183 Site-directed mutagenesis of NEM1 and SPO7, coupled with

184 stal structure of the AT1R was used to guide site-directed mutagenesis of outward-facing hydrophobic

185 Site-directed mutagenesis of phosphorylated residues rev

186 structure and function of CHT7, we performed site-directed mutagenesis of previously identified phosp

187 ucture models such as interpreting/designing site-directed mutagenesis of proteins.

188 Site-directed mutagenesis of PxaTPS8 revealed several ca

189 Site-directed mutagenesis of RadH was used to identify c

190 Site-directed mutagenesis of RCAR1 showed that its tyros

191 Furthermore, by site-directed mutagenesis of residues at the trimerizati

192 Using site-directed mutagenesis of residues in both proteins,

193 Site-directed mutagenesis of residues near the predicted

194 zyme encoded by CHO1 Truncation analysis and site-directed mutagenesis of the CHO1 promoter indicated

195 of N-terminal receptor peptides followed by site-directed mutagenesis of the cleavage sites links re

196 lico findings is validated experimentally by site-directed mutagenesis of the key CcmK2 residue Serin

197 Here, using site-directed mutagenesis of the mitochondrial COX1 gene

198 Site-directed mutagenesis of the phytaspase cleavage sit

199 Site-directed mutagenesis of the Sp1-binding site within

200 By exploiting site-directed mutagenesis of the specific amino acids we

201 affect the activity of G. lovleyi NrfA, and site-directed mutagenesis of this arginine reduced enzym

202 Site-directed mutagenesis of Thr-48 to Ala (T48A) to pre

203 riments, the use of amino acid blockers, and site-directed mutagenesis of UCP1, we propose a mechanis

204 crystallography, in vitro enzyme assays and site-directed mutagenesis, of the bacillaene synthase de

205 Here, we performed site-directed mutagenesis on five envelope (E) protein a

206 In this study, we made site-directed mutagenesis on the surface-exposed hydroph

207 Using site-directed mutagenesis, optical trap-based force spec

208 ties of truncated MT2 that were generated by site-directed mutagenesis or Gibson assembly master mix,

209 Past studies, based on site-directed mutagenesis or solvatochromism of the isol

210 ge of in vitro and rat in situ preparations, site-directed mutagenesis, patch-clamp, nerve recordings

211 n glycosyltransferase structural biology and site-directed mutagenesis, pathway engineering, and deox

212 Site-directed mutagenesis, protein biochemical, and stru

213 Using site-directed mutagenesis, purified recombinant proteins

214 t and its implications for heme transfer via site-directed mutagenesis, resonance Raman (RR), hydroge

215 Site directed mutagenesis results show that residues in

216 oteins by top-down MS, NMR spectroscopy, and site-directed mutagenesis revealed specific and well-con

217 Molecular modeling/simulation coupled with site-directed mutagenesis revealed that Asp(147) or Asn(

218 Site-directed mutagenesis revealed that GRK2 Ser-685 pho

219 Site-directed mutagenesis revealed that Leu(46) and Phe(

220 Site-directed mutagenesis revealed that the McpM precurs

221 mate at 1.94 angstrom resolution, along with site-directed mutagenesis, revealed how the active site

222 C-infected patients (n = 168), together with site-directed mutagenesis, revealed Nef position 9 as a

223 Site-directed mutagenesis reveals that this interaction

224 ogical and biochemical approaches, including site-directed mutagenesis, reverse genetics-based virus

225 Mass spectrometry and site-directed mutagenesis showed that chemically distinc

226 Site-directed mutagenesis showed that Ser-491 is essenti

227 Site-directed mutagenesis showed that the relevant sites

228 ere, using several approaches, including MS, site-directed mutagenesis, siRNA-mediated gene silencing

229 Site-directed mutagenesis studies revealed four substitu

230 Mossbauer spectroscopy measurements and the site-directed mutagenesis studies show that the red Fur

231 Extensive site-directed mutagenesis studies supported the importan

232 Site-directed mutagenesis studies were consistent with b

233 In this study, using computational and site-directed mutagenesis studies, we demonstrate the pr

234 ombined in silico hA(3)AR-homology model and site-directed mutagenesis study was performed to demonst

235 T TtCE15A and variants in which we, by using site-directed mutagenesis, substituted residues suggeste

236 Mechanistic studies using site-directed mutagenesis suggest that, following initia

237 Site-directed mutagenesis supports a model in which aden

238 Using site-directed mutagenesis, surface plasmon resonance, an

239 Site-directed mutagenesis targeting these tyrosine resid

240 cDNAs were constructed by site-directed mutagenesis that encode PORB mutant protei

241 Site-directed mutagenesis that swapped residue 153 betwe

242 Furthermore, we show by site-directed mutagenesis that tyrosine (Y382-384) withi

243 Site-directed mutagenesis then revealed that a previousl

244 Site-directed mutagenesis to block HspB1 phosphorylation

245 n of CD2AP, and phospho-CD2AP antibodies and site-directed mutagenesis to define the specific phospho

246 GAT1 in MGAT4D-L transfectants, we performed site-directed mutagenesis to determine requirements for

247 SagS domain was used as constructs and site-directed mutagenesis to elucidate how SagS performs

248 m different biological kingdoms and employed site-directed mutagenesis to elucidate the molecular bas

249 Here, we have made use of site-directed mutagenesis to examine the contribution of

250 searches for candidate exosites, we utilized site-directed mutagenesis to identify a new exosite in c

251 6 ECR with high-nanomolar affinity, and used site-directed mutagenesis to identify a patch of conserv

252 In the current study, we use site-directed mutagenesis to identify K(100) and K(188)

253 Lon constructs, bioinformatics analysis, and site-directed mutagenesis to identify Lon domains and re

254 evan-type fructooligosaccharide and utilized site-directed mutagenesis to identify residues involved

255 AR1 complex, we used truncation variants and site-directed mutagenesis to investigate domains and res

256 In this report we used site-directed mutagenesis to substitute specific amino a

257 Using site-directed mutagenesis to test multiple potential sit

258 We used X-ray crystallography and site-directed mutagenesis to unravel the molecular and s

259 e (EPR) spectroscopic methods, combined with site-directed mutagenesis, to determine the mechanism of

260 ceptor, and validated this interface through site-directed mutagenesis together with functional and c

261 he effect on STAT4 function was evaluated by site-directed mutagenesis using a lymphoblastoid B cell

262 Site-directed mutagenesis was performed by partial gene

263 Site-directed mutagenesis was subsequently used to ident

264 Site-directed mutagenesis was used to create orthogonal

265 -Golgi enzyme alpha2,6-sialyltransferase and site-directed mutagenesis was used to exchange individua

266 Site-directed mutagenesis was used to investigate the re

267 Site-directed mutagenesis was used to show that the NADH

268 Site-directed mutagenesis was utilized to assess the rol

269 Using site-directed mutagenesis, we confirmed that the predict

270 Using truncation mutants and site-directed mutagenesis, we define the inhibitory face

271 Using site-directed mutagenesis, we demonstrate that the M159I

272 Through site-directed mutagenesis, we demonstrate that these res

273 Using site-directed mutagenesis, we demonstrated that the cons

274 Using site-directed mutagenesis, we determined that glycosylat

275 equence alignment, pH-activity profiles, and site-directed mutagenesis, we evaluated a series of acti

276 nce resonance energy transfer technique, and site-directed mutagenesis, we examined the domains invol

277 with purified and recombinant proteins, and site-directed mutagenesis, we examined the role of a put

278 Using site-directed mutagenesis, we found that mutations predi

279 r fulcrum." While validating the model using site-directed mutagenesis, we found that the Tyr-542 res

280 Using site-directed mutagenesis, we further defined this addit

281 Using site-directed mutagenesis, we generated FOXM1 mutant pro

282 gh a combination of in silico prediction and site-directed mutagenesis, we have mapped an exosite to

283 sing homology modeling of ENaC structure and site-directed mutagenesis, we identified a cleft region

284 Here, by using site-directed mutagenesis, we identified Arg-8 of subuni

285 Using in silico docking and site-directed mutagenesis, we identified Asp(230) in the

286 Using site-directed mutagenesis, we identified serine-465 as t

287 In addition, using site-directed mutagenesis, we identify those amino acids

288 Through extensive site-directed mutagenesis, we map phosphorylation to the

289 Using site-directed mutagenesis, we mutated the specific sites

290 in interaction and kinetic assays as well as site-directed mutagenesis, we provide further insight in

291 using protein-lipid co-flotation assays and site-directed mutagenesis, we report that the N-region o

292 ioinformatics-based approaches combined with site-directed mutagenesis, we show here that SLFN11 is p

293 nalyses, molecular dynamics simulations, and site-directed mutagenesis, we show that 8-NBD-cGMP inhib

294 Here, using site-directed mutagenesis, we show that Asn-26 in the mo

295 Through site-directed mutagenesis, we show that three amino acid

296 Using site-directed mutagenesis, we validated those surfaces i

297 Finally, in vitro PLK1 kinase assays and site-directed mutagenesis were employed to demonstrate t

298 subcellular localization were identified by site-directed mutagenesis, which identified serine resid

299 test the transition state model, we combined site-directed mutagenesis with kinetic experiments, yiel

300 ionary lineage analysis and structure-guided site-directed mutagenesis with large-scale functional si