ライフサイエンスコーパス: site-specific mutagenesis

1 econdarily to test the functional impacts of site-specific mutagenesis.

2 y fluorescence resonance energy transfer and site-specific mutagenesis.

3 ytic essentiality of Glu-216 was revealed by site-specific mutagenesis.

4 and SAA2 promoters that had been modified by site-specific mutagenesis.

5 ed in combination with urea denaturation and site-specific mutagenesis.

6 ule and is investigated by replacement using site-specific mutagenesis.

7 cicum, respectively, have been identified by site-specific mutagenesis.

8 bamoylase has been replaced by alanine using site-specific mutagenesis.

9 s of the regulatory chain were deleted using site-specific mutagenesis.

10 We tested these predictions using site-specific mutagenesis.

11 on sites in C epsilon 3 were now targeted by site-specific mutagenesis.

12 e when the HIV-1 protease was inactivated by site-specific mutagenesis.

13 the mechanism of its formation was probed by site-specific mutagenesis.

14 ndividually and together were constructed by site-specific mutagenesis.

15 inhibitory site was identified as Thr108 by site-specific mutagenesis.

16 r heterotypic interactions by deletional and site-specific mutagenesis.

17 I-II linker were eliminated by deletion and site-specific mutagenesis.

18 transducer for sensory rhodopsin I (SRI)] by site-specific mutagenesis.

19 oxylase were substituted with alanines using site-specific mutagenesis.

20 surface charge by chemical supercharging or site-specific mutagenesis.

21 ractions by protein-protein crosslinking and site-specific mutagenesis.

22 zation of the MRN complex were identified by site-specific mutagenesis.

23 ure-jump (T-jump) method in conjunction with site-specific mutagenesis.

24 sporter protein were altered individually by site-specific mutagenesis.

25 by introducing cysteines at these sites with site-specific mutagenesis.

26 We constructed by site-specific mutagenesis a mutant, dnaQ926, by changing

27 Site-specific mutagenesis along with the analysis of all

28 Deletion and site-specific mutagenesis analysis identified clustered

29 logy modeling, electrostatic calculation and site-specific mutagenesis analysis to identify a positiv

30 Through site-specific mutagenesis and a pH increase from 8.5 to

31 ple and powerful genome engineering tool for site-specific mutagenesis and allelic replacement in yea

32 Here we demonstrate by site-specific mutagenesis and analysis of phototaxis beh

33 e structural predictions, we show here using site-specific mutagenesis and ATP docking simulations th

34 By site-specific mutagenesis and binding site selection, we

35 are an analogue of phosphorylated CheB using site-specific mutagenesis and chemical modification stra

36 ZFN-mediated site-specific mutagenesis and complete removal of the GF

37 using computer simulations supplemented with site-specific mutagenesis and crosslinking of the alpha9

38 Site-specific mutagenesis and deletion analyses demonstr

39 Site-specific mutagenesis and deletion analysis confirm

40 By site-specific mutagenesis and deletion analysis, we iden

41 rted crystal structure of SarR, we conducted site-specific mutagenesis and demonstrate that K52 resid

42 ese mutations, were created through in vitro site-specific mutagenesis and expressed in Escherichia c

43 have analyzed the role of the CArG boxes by site-specific mutagenesis and find that the three CArG b

44 The third aspect employed site-specific mutagenesis and functional assays.

45 rrel with a central hydrophilic channel, and site-specific mutagenesis and fusion protein analysis de

46 -AAV6-based genome editing tool platform for site-specific mutagenesis and integration of up to more

47 he function of this loop in Csk by extensive site-specific mutagenesis and kinetic studies using phys

48 Site-specific mutagenesis and N-terminal sequencing loca

49 ractionation, and domain-mapping assays with site-specific mutagenesis and purified recombinant prote

50 leotides (TFOs) provide a means for inducing site-specific mutagenesis and recombination at chromosom

51 Recent work has suggested that site-specific mutagenesis and recombination might be ach

52 Site-specific mutagenesis and selective MAPK inhibitors

53 On the basis of site-specific mutagenesis and specific 15N-decoupling, t

54 The work described here used site-specific mutagenesis and spectroscopy to confirm th

55 Site-specific mutagenesis and steady-state kinetic analy

56 This report describes additional site-specific mutagenesis and synthetic peptide inhibiti

57 ila Notch, we conducted deletion mapping and site-specific mutagenesis and then assayed the binding o

58 e VZV and model promoters was examined using site-specific mutagenesis and transfection and superinfe

59 Site-specific mutagenesis and transfection studies revea

60 Here we use site-specific mutagenesis and two-dimensional NMR of l-[

61 nds have been examined with a combination of site-specific mutagenesis and X-ray crystallography.

62 3.4 A resolution, complemented by extensive site-specific mutagenesis, and a hybrid model of glucago

63 h stress markers TIA-1, CUGBP1, and ph-eIF2, site-specific mutagenesis, and examinations of RNA-prote

64 dies and later confirmed by crystallography, site-specific mutagenesis, and molecular modeling: an ac

65 Sequence comparisons, the results of site-specific mutagenesis, and tests of chemical stabili

66 ae Gpi3p, as well as Cys301, were altered by site-specific mutagenesis, and the mutant proteins teste

67 ed both its length and flexibility in HSO by site-specific mutagenesis, and the reactivities of the r

68 The current study used a site-specific mutagenesis approach to determine the role

69 denosine (epsilondA) in human cells, a novel site-specific mutagenesis approach was developed, in whi

70 In vitro kinase and site-specific mutagenesis approaches indicate that MST1

71 Using site-specific mutagenesis, Asp51 was mutated both to ala

72 We have developed and validated a novel site-specific mutagenesis assay, termed SSMA-MS, which i

73 in vivo at the level of sensitivity of these site-specific mutagenesis assays.

74 group (Family VI) in the periodic table, the site-specific mutagenesis at the atomic level by replaci

75 ead to increased homologous recombination or site-specific mutagenesis at the repair site.

76 when the proton donor Asp-96 is removed with site-specific mutagenesis, but its rate is restored upon

77 This technique is advantageous over site-specific mutagenesis by allowing side-by-side selec

78 Site-specific mutagenesis by psoralen cross-links was de

79 sharp resonances that have been assigned by site-specific mutagenesis by replacing each 3-fluorotyro

80 N-terminal metal-binding site is disabled by site-specific mutagenesis, can only bind one metal ion.

81 Site-specific mutagenesis combined with functional chara

82 Site-specific mutagenesis combined with Western blot ana

83 Site-specific mutagenesis coupled with enzyme kinetics w

84 Here, we use site-specific mutagenesis, coupled with calorimetric, NM

85 We used site-specific mutagenesis, coupled with phosphorylation

86 y and bay region lesions are correlated with site-specific mutagenesis data.

87 Site-specific mutagenesis demonstrated that the sequence

88 CE1 site-specific mutagenesis determined that it binds NF1-

89 domain swapping and interspecies reciprocal site-specific mutagenesis determined that the phenotypic

90 Sequencing and site-specific mutagenesis determined that the syncytium-

91 mber of gene targeting applications, such as site-specific mutagenesis, development of transgenic ani

92 As verified by site-specific mutagenesis, disulfide linkages can form b

93 Site-specific mutagenesis does not support suggestions m

94 identified amino acid was validated through site-specific mutagenesis, electrophysiology functional

95 Site-specific mutagenesis established that both sites ac

96 h chromatin immunoprecipitation and promoter site-specific mutagenesis evidence linking HMGA1 to repr

97 s dispersion and used the structure to guide site-specific mutagenesis experiments addressing substra

98 Site-specific mutagenesis experiments have confirmed tha

99 nsversions and epsilon dC-->T transitions in site-specific mutagenesis experiments in mammalian cells

100 Supporting this mechanism, site-specific mutagenesis experiments show that ClC-ec1

101 Results from site-specific mutagenesis experiments showed that the ac

102 Previous site-specific mutagenesis experiments showed that when G

103 Results of kinetics and site-specific mutagenesis experiments suggest that this

104 The results were correlated with site-specific mutagenesis experiments that revealed the

105 itions observed 5' to the modified (AFB)G in site-specific mutagenesis experiments.

106 idate the predicted structure with data from site-specific mutagenesis experiments.

107 Each of the mutant enzymes was re-created by site-specific mutagenesis, expressed, purified, and kine

108 f the glycosylation recognition motifs using site-specific mutagenesis, followed by cryoelectron micr

109 I gene promoter was analyzed by deletion and site-specific mutagenesis following transient transfecti

110 erminal glycine of the sorghum C4 isoform by site-specific mutagenesis (G961(A/V/D)) and truncation (

111 Site-specific mutagenesis has been used to alter a porti

112 Site-specific mutagenesis has been used to change the co

113 Site-specific mutagenesis has been used to introduce sin

114 Site-specific mutagenesis has been used to replace His-4

115 Incorporation of 3-fluorotyrosine and site-specific mutagenesis has been utilized with Fourier

116 over the distance between the two sites, but site-specific mutagenesis has failed to reveal residues

117 Previously, site-specific mutagenesis has yielded reaction centers c

118 that expresses a functional CXCR4 receptor, site-specific mutagenesis, hybrid CXCR3/CXCR4 receptors,

119 Site-specific mutagenesis identified His-20 as the proxi

120 Site-specific mutagenesis identifies that the lysine res

121 This model was used to select residues for site-specific mutagenesis in an effort to identify resid

122 troduction of STAT3 DNA-binding sequences by site-specific mutagenesis in an immunostimulatory gene p

123 hand, has been utilized for the induction of site-specific mutagenesis in plants.

124 n of the size of two hydrophobic residues by site-specific mutagenesis in SLO reduces the reaction ra

125 Site-specific mutagenesis indicates that residue threoni

126 By site-specific mutagenesis it was shown that hydrophobic

127 Finally, a combination of site-specific mutagenesis, mass spectrometry, and in sil

128 eine6 with adjacent serines (rSP-C[Ser]2) by site-specific mutagenesis minimized aggregation of rSP-C

129 The combination of site specific mutagenesis of the three ZDHHC6 palmitoyla

130 modified, psoralen-linked TFOs that mediate site-specific mutagenesis of a chromosomal gene in livin

131 Site-specific mutagenesis of a GATA transcription factor

132 Site-specific mutagenesis of all amino acids comprising

133 iants of DNA or RNA were used as primers for site-specific mutagenesis of bacteriophage f1.

134 scriptional activity in reporter assays, and site-specific mutagenesis of c-Fos and Nfat elements abr

135 Using site-specific mutagenesis of channel subunits, ENaC expr

136 Site-specific mutagenesis of cloned TCR genes and transf

137 The results of overexpression and site-specific mutagenesis of CnRHO1 in C. neoformans and

138 ic analysis of other superfamily members and site-specific mutagenesis of E. coli MurG, this structur

139 Site-specific mutagenesis of each of the HNF-3 binding s

140 However, site-specific mutagenesis of eight His residues to Gln i

141 Site-specific mutagenesis of either of the TTF-1 binding

142 ed these interactions using a combination of site-specific mutagenesis of Escherichia coli DNA polyme

143 Finally, site-specific mutagenesis of functional NF-kappaB sites

144 ructed 15 conservative alterations of PII by site-specific mutagenesis of glnB and also isolated thre

145 des when Cys452 was replaced with alanine by site-specific mutagenesis of human PKCepsilon or a const

146 and bovine RNase A, has been investigated by site-specific mutagenesis of human RI and Ang.

147 Site-specific mutagenesis of individual residues identif

148 Here, we describe a protocol using site-specific mutagenesis of individual residues respons

149 analyzing mutations generated by random and site-specific mutagenesis of luxI.

150 Site-specific mutagenesis of Lys-179 in motif I abolishe

151 Sequence analysis and site-specific mutagenesis of N-tropic MLVs identified a

152 In this work we have performed site-specific mutagenesis of potential N-linked glycosyl

153 Site-specific mutagenesis of predicted catalytic residue

154 Through a programme of random and site-specific mutagenesis of rhII we have gained a bette

155 e enzymatic parameters of RrmJ and conducted site-specific mutagenesis of RrmJ.

156 The effect of eliminating phosphorylation by site-specific mutagenesis of serines 58 and 61 on the fu

157 double-nicking CRISPR/Cas9 system to conduct site-specific mutagenesis of SNCA in these cells, genera

158 tes should improve, allowing confirmation by site-specific mutagenesis of surface-exposed residues.

159 However, site-specific mutagenesis of the -35 element of the puta

160 Internal deletion and site-specific mutagenesis of the 100-bp fragment identif

161 , and these activities were distinguished by site-specific mutagenesis of the active site residues of

162 Using site-specific mutagenesis of the bovine protein and expr

163 Site-specific mutagenesis of the carboxyl-terminal serin

164 rogressive deletional analyses together with site-specific mutagenesis of the E-selectin promoter ind

165 Site-specific mutagenesis of the first GATA1 binding sit

166 By site-specific mutagenesis of the gene 8 mRNA, residues a

167 Site-specific mutagenesis of the hmfB gene cloned from t

168 Site-specific mutagenesis of the identified PON1 residue

169 Nuclease mapping and site-specific mutagenesis of the minimal RNA sequence de

170 anslational regulation of gp63 expression by site-specific mutagenesis of the predicted catalytic/zin

171 Site-specific mutagenesis of the predicted protease acti

172 e triphosphate (dNTP) using a combination of site-specific mutagenesis of the protein and atomic subs

173 Site-specific mutagenesis of the putative phosphorylatio

174 Site-specific mutagenesis of the start codons for ExsB a

175 ansfection of miRNA mimics or inhibitors and site-specific mutagenesis of their 3'-untranslated regio

176 Site-specific mutagenesis of these cysteine residues on

177 We have carried out site-specific mutagenesis of these five histidine residu

178 ith furin-specific inhibitor experiments, by site-specific mutagenesis of Tractin constructs expresse

179 Site-specific mutagenesis of two identified stress resis

180 Site-specific mutagenesis of two pairs of residues in th

181 c activity of the protein was inactivated by site-specific mutagenesis or inhibited by zinc chelation

182 reover, depletion of Src via gene silencing, site-specific mutagenesis, or pharmacological inhibition

183 Site-specific mutagenesis performed at positions 182 and

184 5 in M. tuberculosis and M. bovis BCG, using site-specific mutagenesis, promoter fusions and reverse-

185 alysis, genome mining, isotope labelling and site-specific mutagenesis rationalize the commonality in

186 of modification is at Tyr105 on the basis of site-specific mutagenesis results.

187 Site-specific mutagenesis revealed that a single lysine

188 Site-specific mutagenesis revealed that arginine at posi

189 ated ATPase activity of hSWS1-SWSAP1 through site-specific mutagenesis, revealing that DNA with an ex

190 gle disulfide bonds in kappa-bungarotoxin by site-specific mutagenesis reveals a differential role fo

191 A manipulations such as directional cloning, site-specific mutagenesis, sequence insertion or deletio

192 ve charge at the active center is removed by site-specific mutagenesis share this characteristic of t

193 Extensive site-specific mutagenesis shows that ice-binding activit

194 Site-specific mutagenesis shows that Thr-213 is catalyti

195 Site-specific mutagenesis shows the HA1 Asp-225-->Asn su

196 Site-specific mutagenesis studies demonstrate that amino

197 cations at multiple sites, including Trp(72) Site-specific mutagenesis studies have suggested, but ha

198 Our deletion and site-specific mutagenesis studies identify serines 158 a

199 Site-specific mutagenesis studies indicate that oxazolon

200 Previous site-specific mutagenesis studies of PYP in our laborato

201 DNA replication past 1,N(2)-epsilon G and site-specific mutagenesis studies on mammalian cells hav

202 Further site-specific mutagenesis studies revealed that the alte

203 Subsequent site-specific mutagenesis studies showed that mutations

204 Site-specific mutagenesis studies suggest that a C-termi

205 Site-specific mutagenesis studies suggest that Glu-4 is

206 The results are discussed in the context of site-specific mutagenesis studies which reveal that the

207 Site-specific mutagenesis suggested the involvement of c

208 Site-specific mutagenesis suggests that this is due to a

209 Here we exploit an in vivo, site-specific mutagenesis system to create short inserti

210 Site-specific mutagenesis that altered surface residues

211 We demonstrate by phosphopeptide mapping and site-specific mutagenesis that rhIKK-i and rhTBK-1 are p

212 ared from a set of rNspA variants created by site-specific mutagenesis, the most important contacts b

213 he reaction conditions for these enzymes and site-specific mutagenesis to alter end product specifici

214 porcine submaxillary mucin were modified by site-specific mutagenesis to assess the roles of individ

215 pa B by using TNFs that has been designed by site-specific mutagenesis to bind either the p60 (R32W;

216 We used limited site-specific mutagenesis to characterize the strongest

217 ction of many transcription factors, we used site-specific mutagenesis to delineate the roles of thes

218 sion in Nicotiana benthamiana leaf cells and site-specific mutagenesis to determine how TGB protein i

219 We employed both random and site-specific mutagenesis to determine the function of a

220 n this region has therefore been examined by site-specific mutagenesis to determine which residues ac

221 the use of Raman spectroscopy combined with site-specific mutagenesis to document the existence of f

222 Here we use site-specific mutagenesis to eliminate the strong proxim

223 We have used site-specific mutagenesis to elucidate the limit domain

224 We used site-specific mutagenesis to evaluate the contributions

225 As proof-of-principle, we used site-specific mutagenesis to examine the role of NEMO in

226 Our results provide the structural basis for site-specific mutagenesis to further improve the binding

227 Here, we used deletion and site-specific mutagenesis to generate mutant Nef protein

228 steine residue near the rim of the P-site by site-specific mutagenesis to generate recombinant human

229 -S clusters and which can be changed through site-specific mutagenesis to glycine residues, and the u

230 In the current study we combined random and site-specific mutagenesis to identify amino acid residue

231 th strains expressing fHbp polymorphisms and site-specific mutagenesis to identify residues that are

232 We used site-specific mutagenesis to make substitutions for thes

233 Site-specific mutagenesis to prevent proteolytic process

234 , I133L and L140A, were made individually by site-specific mutagenesis to produce two mutant proteins

235 We used site-specific mutagenesis to reduce the subsite specific

236 Here, we used site-specific mutagenesis to target the coiled-coil hept

237 e catalytic role of R277 was investigated by site-specific mutagenesis together with chemical rescue

238 e was altered to a glycine residue by guided site-specific mutagenesis using a synthetic oligonucleot

239 Oligonucleotide-directed site-specific mutagenesis was carried out on pyruvate de

240 Oligonucleotide-directed site-specific mutagenesis was carried out on pyruvate de

241 Oligonucleotide-directed site-specific mutagenesis was carried out on pyruvate de

242 Oligonucleotide-directed site-specific mutagenesis was carried out on pyruvate de

243 In this report site-specific mutagenesis was employed to analyze the na

244 Site-specific mutagenesis was employed to change, one at

245 Site-specific mutagenesis was performed on the latter RN

246 In this study site-specific mutagenesis was used to assess the contrib

247 Site-specific mutagenesis was used to convert bacterial

248 Site-specific mutagenesis was used to create CD44 mutant

249 ular loops exhibited amino acid differences, site-specific mutagenesis was used to exchange the key A

250 Site-specific mutagenesis was used to investigate the ro

251 Using localized and site-specific mutagenesis we have identified a functiona

252 By site-specific mutagenesis we previously demonstrated tha

253 By using site-specific mutagenesis we showed that an active-site

254 Based on site specific mutagenesis, we propose that phospho-Ser17

255 By site-specific mutagenesis, we changed the lysines of the

256 Through site-specific mutagenesis, we defined the following sets

257 Furthermore, using site-specific mutagenesis, we demonstrated that the firs

258 Using site-specific mutagenesis, we demonstrated that Tyr(233)

259 Through site-specific mutagenesis, we examined the determinants

260 By site-specific mutagenesis, we found that the tyrosine 24

261 Using confocal microscopy and site-specific mutagenesis, we have determined that the p

262 Using site-specific mutagenesis, we identified in the earliest

263 Using oligonucleotide-directed site-specific mutagenesis, we introduced base substituti

264 In this study, by sequence alignment and site-specific mutagenesis, we located a substrate-dockin

265 Using deletion and site-specific mutagenesis, we report that IL-17-mediated

266 Using site-specific mutagenesis, we show here that the amino-a

267 Using site-specific mutagenesis, we showed that the conserved

268 based codon-specific random mutagenesis, and site-specific mutagenesis were performed to construct a

269 Deletion and site-specific mutagenesis were used to identify multiple

270 responsible residue is usually identified by site-specific mutagenesis, which may be time-consuming.

271 Using a combination of random and site-specific mutagenesis with zinc K-edge extended X-ra

272 Site-specific mutagenesis within M2 revealed that D137 a

273 We have used site-specific mutagenesis within the extracellular domai

274 ct to A-MuLV receptor function by performing site-specific mutagenesis within this region of Pit2.