ライフサイエンスコーパス: site-specific recombination

1 (via repair of paused replication forks and site-specific recombination).

2 roles in transcriptional regulation and DNA site-specific recombination.

3 and its subsequent deletion by FLP-mediated, site-specific recombination.

4 he attB locus in the S. aurantiaca genome by site-specific recombination.

5 ulation, supercoil and catenane removal, and site-specific recombination.

6 programmed to excise from the chromosome by site-specific recombination.

7 to and out of the bacterial chromosome using site-specific recombination.

8 er-order protein-DNA complexes necessary for site-specific recombination.

9 y inactivating the promoter, for example, by site-specific recombination.

10 y (CHO) cells using Flp recombinase-mediated site-specific recombination.

11 probe the function of accessory proteins in site-specific recombination.

12 -bound alkaline phosphatase reporter gene by site-specific recombination.

13 xtra copies of the transgene were deleted by site-specific recombination.

14 t of recA, suggesting that it is mediated by site-specific recombination.

15 gration and excision are regulated in lambda site-specific recombination.

16 homopolymeric sequence repeats or motifs for site-specific recombination.

17 ls of complexity in the regulation of lambda site-specific recombination.

18 polymerase (T7RNAP), and (iii) FLP-mediated site-specific recombination.

19 nto BAC/PAC vectors through in vivo cre/loxP site-specific recombination.

20 with a linked gene of interest by the use of site-specific recombination.

21 interactions and host enzymes, resulting in site-specific recombination.

22 capable of undergoing growth phase-dependent site-specific recombination.

23 erived from microorganisms mediate efficient site-specific recombination.

24 ems that are associated with replication and site-specific recombination.

25 e selection marker gene by Cre-loxP-mediated site-specific recombination.

26 er of supercoils trapped by lambda integrase site-specific recombination.

27 dye-labeled protein-DNA intermediates in Cre site-specific recombination.

28 assessed using a genetic assay for Cre/loxP site-specific recombination.

29 host, Myxococcus xanthus, by a mechanism of site-specific recombination.

30 data, transcriptomics, synthetic biology and site-specific recombination.

31 s efficient manipulation of targeted loci by site-specific recombination.

32 he plant genome, we used a strategy based on site-specific recombination.

33 nce for the proposed rotational mechanism of site-specific recombination.

34 onase (Xis) protein is required for excisive site-specific recombination.

35 d mosquito chromosomal "docking" sites using site-specific recombination.

36 defective for lysis but fully competent for site-specific recombination.

37 can act as substrates for further rounds of site-specific recombination.

38 roteins act to convert dimers to monomers by site-specific recombination.

39 GI may be acquired or lost by XerCD-mediated site-specific recombination.

40 inus region, ter, where FtsK activates XerCD site-specific recombination.

41 tion in a non-sequence-directed process, and site-specific recombination.

42 ion or more plausibly via integrase-mediated site-specific recombination.

43 inserted into a defined genomic location by site-specific recombination.

44 nsfer gene sequences between vectors through site-specific recombination.

45 DNA that is tagged with sequence targets for site-specific recombination.

46 t)-regulatable promoter through Cre-mediated site-specific recombination.

47 nstead be accomplished by multiple rounds of site-specific recombination.

48 defined DNA segments commonly occur through site-specific recombination, a process of DNA breakage a

49 f plasmid ColE1 are converted to monomers by site-specific recombination, a process that requires 240

50 trated that this sequence was sufficient for site-specific recombination after infection with transdu

51 Site-specific recombination also takes place in females,

52 two mutants, W350A and I353A, cannot perform site-specific recombination although their DNA binding,

53 s genetic processes including homologous and site-specific recombination and DNA replication.

54 ious genetic processes including homologous, site-specific recombination and DNA replication.

55 These include slipped strand mispairing, site-specific recombination and epigenetic regulation me

56 integrated into the gonococcal chromosome by site-specific recombination and may be lost by site-spec

57 roteins that serve as essential cofactors in site-specific recombination and nucleoid organization an

58 ase Integrase (Int) can catalyze integrative site-specific recombination and recombinase-mediated cas

59 were constructed and examined by integrative site-specific recombination and RMCE assays in human cel

60 determine the specific base requirements for site-specific recombination and showed that specificity

61 cting trs sequence is not a prerequisite for site-specific recombination and suggests AAV targeting i

62 Thus, this mechanism serves to regulate Mx8 site-specific recombination and superinfection immunity

63 very, TFOs may be effective tools to promote site-specific recombination and targeted modification of

64 icant impact toward the understanding of AAV site-specific recombination and the development of targe

65 ulatory cofactors in many processes, such as site-specific recombination and the initiation of replic

66 was excised from the chromosome by inducible site-specific recombination and tracked by real-time flu

67 o unrelated systems in Escherichia coli: Xer site-specific recombination and transcriptional regulati

68 From bacterial viruses to humans, site-specific recombination and transposition are the ma

69 ion initiation, transcription regulation and site-specific recombination, and is associated with bact

70 hese expression systems, in combination with site-specific recombination approaches, have also led to

71 quences and chromatin structure required for site-specific recombination are contained within this fr

72 erlying its binding to DNA, DNA bending, and site-specific recombination are fundamentally different

73 small, compact, and simple switches that use site-specific recombination as the key decision point.

74 We have used a site-specific recombination assay in Saccharomyces cerev

75 romyces pombe by application of the Cre/loxP site-specific recombination assay.

76 Xer site-specific recombination at dif, a 28-bp site located

77 tes chromosome unlinking by activating XerCD site-specific recombination at dif, located in the repli

78 ZFRs can be tailored to promote site-specific recombination at diverse 'Z-sites', which

79 asmids are integrated into the chromosome by site-specific recombination at one of five different pha

80 integrating into the bacterial chromosome by site-specific recombination at one of two sites, attB1 o

81 evolved regulatory contacts that coordinate site-specific recombination at the C-terminus.

82 ell division to be resolved into monomers by site-specific recombination at the dif locus of Escheric

83 Xer site-specific recombination at the Escherichia coli chro

84 XerCD site-specific recombination at the Escherichia coli dif

85 Xer site-specific recombination at the psi site from plasmid

86 Although site-specific recombination-based cloning systems, such

87 Among the available methods, site-specific recombination-based cloning techniques, wh

88 n of bacteriophage DNA into a host genome by site-specific recombination between 'attachment sites' i

89 Cre recombinase catalyzes site-specific recombination between 34-bp loxP sites in

90 the chromosome of its Streptomyces host, by site-specific recombination between attP (the attachment

91 re-recombinase of bacteriophage P1 catalyses site-specific recombination between DNA fragments contai

92 s temperate bacteriophage varphiC31, promote site-specific recombination between DNA sequences in the

93 The feasibility of site-specific recombination between genomes of different

94 nd mammalian cells, mediating unidirectional site-specific recombination between its attB and attP re

95 cteriophage lambda integrase (Int) catalyzes site-specific recombination between pairs of attachment

96 a conditional origin of replication promotes site-specific recombination between the FRT sites, resul

97 Site-specific recombination between the terminal transge

98 oblast cells, Cre was expressed and mediated site-specific recombination between the two LoxP sites,

99 Cre recombinase catalyzes site-specific recombination between two 34-bp loxP sites

100 Phage integrases mediate efficient site-specific recombination between two different sequen

101 ases are enzymes that mediate unidirectional site-specific recombination between two DNA recognition

102 at inside oriT; the protein also facilitated site-specific recombination between two oriT2 sites.

103 Flp site-specific recombination between two target sites (FR

104 Therefore, Piv may mediate site-specific recombination by a novel mechanism.

105 id procedure for DNA fragment assembly using site-specific recombination by C31 integrase.

106 The RAG1-RAG2 protein complex initiates this site-specific recombination by cutting DNA at specific s

107 gration host factor (IHF) facilitates lambda site-specific recombination by inducing DNA bends necess

108 Site-specific recombination by phages lambda and P22 is

109 determines the outcome of integrase-mediated site-specific recombination by redesign of higher-order

110 Xis modulates the directionality of site-specific recombination by stimulating phage excisio

111 Site-specific recombination by the Cre recombinase takes

112 on sites interspersed with two res sites for site-specific recombination by Tn21 resolvase, in buffer

113 sites interspersed with two 21res sites for site-specific recombination by Tn21 resolvase; inhibitio

114 We show that step-wise, site-specific recombination by XerCD-dif or Cre-loxP can

115 Therefore, Flp-FRT site-specific recombination can be applied to switch RD

116 ually occur in the male Drosophila germline, site-specific recombination can be induced at the ends o

117 e show that, under proper guidance, Cre-loxP site-specific recombination can mediate efficient trans-

118 We conclude that phage integrase-mediated site-specific recombination can produce iPS cells that h

119 mplexes, known as intasomes, is required for site-specific recombination catalysed by bacteriophage L

120 of DNA repair, replication fork restart, and site-specific recombination catalysed by tyrosine recomb

121 Site-specific recombination catalyzed by bacteriophage l

122 Here, we present a topological model of site-specific recombination characterizing all possible

123 the absence of the topoisomerase topoIV, the site-specific recombination complex XerCD- dif-FtsK can

124 ssion vector pDEST17, necessary for in vitro site-specific recombination, contains the sequence AAA-A

125 Escherichia coli Xer site-specific recombination converts chromosomal and pla

126 During the first steps of site-specific recombination, Cre protein cleaves and rel

127 emoved from integrated transgenes in vivo by site-specific recombination demonstrated that MAR sequen

128 n by increasing the affinity of TorI for its site-specific recombination DNA target.

129 s are critical intermediates for homologous, site-specific recombination, DNA repair, and replication

130 an be efficiently excised by Flp recombinase site-specific recombination, either when Flp is expresse

131 the initial (rate-limiting) step involves a site-specific recombination event involving plasmid-enco

132 tion of this cluster is missing because of a site-specific recombination event that occurred between

133 every generation by a highly choreographed, site-specific recombination event that replaces one MAT

134 Prophage excision involves a second site-specific recombination event, in which the sites ge

135 acterial hosts through an integrase-mediated site-specific recombination event.

136 Both the integrative and excisive site-specific recombination events are catalyzed by the

137 ased system with potential for regulation of site-specific recombination events at the protein level

138 f Int are presumed to be important for these site-specific recombination events for several reasons:

139 of the SXT element shares many features with site-specific recombination found in lambdoid phages.

140 transfer, without requiring transposition or site-specific recombination functions.

141 Inactivation of hmuT in C. diphtheriae by site-specific recombination had no effect on hemin utili

142 A strategy involving homologous and site-specific recombination has been devised by which si

143 mpted the development of several strategies (site-specific recombination, homologous recombination, t

144 Many natural systems of site-specific recombination impose sophisticated regulat

145 osomal integration of the element occurs via site-specific recombination in a 17 bp sequence found in

146 Flp catalyzes site-specific recombination in a highly sequence-specifi

147 volved clones, GinL7C7, catalyzed efficient, site-specific recombination in a variety of sequence con

148 e recombinase gamma delta resolvase performs site-specific recombination in an elaborate synaptic com

149 thered to a TFO domain were found to mediate site-specific recombination in an intracellular SV40 vec

150 n shown in numerous instances to mediate lox site-specific recombination in animal and plant cells.

151 In addition, results obtained using site-specific recombination in bacteria and chromosome c

152 nce the original description of Cre mediated site-specific recombination in bacteriophage P1, the Cre

153 Xer site-specific recombination in Escherichia coli converts

154 ncoded IntX integrase acts to limit excisive site-specific recombination in lysogens carrying a singl

155 ated into genomic loxP sites by Cre-mediated site-specific recombination in mammalian cells.

156 a method that combines the genetic power of site-specific recombination in order to selectively "tag

157 an be used to obtain rapid, highly regulated site-specific recombination in P. falciparum, capable of

158 The FLP recombinase promotes site-specific recombination in the 2 micrometer circle o

159 omotes efficient reciprocal and conservative site-specific recombination in vertebrate cells and in S

160 shown to be involved in bacteriophage lambda site-specific recombination in vivo, enhancing the level

161 liday junction intermediates of phage lambda site-specific recombination in vivo.

162 ith a sporozoite cDNA library and cloned via site-specific recombination into a bacterial shuttle vec

163 As a tool in directed genome manipulations, site-specific recombination is a double-edged sword.

164 fusions to the lac operon using FLP mediated site-specific recombination is described.

165 Site-specific recombination is involved in processes ran

166 Catalysis of site-specific recombination is preceded by the formation

167 Site-specific recombination is responsible for a broad r

168 Site-specific recombination is the basis for male recomb

169 DNA and important cellular processes such as site-specific recombination is very limited.

170 monella chromosome via an integrase-mediated site-specific recombination mechanism.

171 tegrase encoded within the specific PAI by a site-specific recombination mechanism.

172 ocated within 5 bp, providing evidence for a site-specific recombination mechanism.

173 ate into a specific genome target site via a site-specific recombination mechanism.

174 ggesting that IS492 transposition involves a site-specific recombination mechanism.

175 an use this program to compute and visualize site-specific recombination mechanisms that accommodate

176 Prophage excision occurs through site-specific recombination mediated by a prophage-encod

177 ocations induced by two distinct mechanisms, site-specific recombination mediated by Cre or non-homol

178 cles than in vitro DNA shuffling and, unlike site-specific recombination methods, allows for recombin

179 Xer site-specific recombination occurred relatively efficien

180 Although Int-mediated site-specific recombination occurs between attP and eith

181 Site-specific recombination of a disrupted gidA gene int

182 on host factor (IHF) protein is required for site-specific recombination of bacteriophage lambda DNA.

183 n the CDK2 and CDK1 genes resulted in a >85% site-specific recombination of Neo-resistant clones vers

184 cis selfishness and trans retaliations; (5) site-specific recombination of plasmid dimers is equival

185 inases that mediate integrative and excisive site-specific recombination of temperate phage genomes.

186 ologous DNA double-stranded break repair and site-specific recombination of V(D)J gene segments.

187 This nonhomologous, site-specific recombination of viral DNA with the human

188 We used site-specific recombination on a dispensable chromosome

189 This model studies generic site-specific recombination on arbitrary twist knot subs

190 Site-specific recombination on supercoiled circular DNA

191 Site-specific recombination on supercoiled circular DNA

192 te-specific recombination and may be lost by site-specific recombination or natural transformation.

193 The site-specific recombination pathway by which the bacteri

194 al cleavage and strand exchange steps in the site-specific recombination pathway.

195 mbda integrase (Int) catalyzes at least four site-specific recombination pathways between pairs of at

196 acteriophage lambda integrase catalyzes four site-specific recombination pathways with distinct prote

197 ediates in both homologous recombination and site-specific recombination performed by tyrosine recomb

198 Site-specific recombination plays key roles in microbe b

199 Details of the site-specific recombination processes have been revealed

200 duction of a bacteriophage lambda lysogen, a site-specific recombination reaction excises the phage g

201 apeptides which inhibit different steps in a site-specific recombination reaction mediated by the bac

202 The Hin recombinase catalyzes a site-specific recombination reaction that results in the

203 ed, and its ability to participate in a full site-specific recombination reaction was reduced only sl

204 In a subsequent site-specific recombination reaction, a gene of interest

205 gene segments in developing lymphocytes by a site-specific recombination reaction, V(D)J recombinatio

206 lex regulatory patterns associated with this site-specific recombination reaction.

207 expressing cells, allowing assessment of the site-specific recombination reaction.

208 For the site-specific recombination reactions catalyzed by the b

209 new proteins that confer directionality upon site-specific recombination reactions encoded by plasmid

210 A fundamental step in site-specific recombination reactions involves the forma

211 owever, what makes the reaction unique among site-specific recombination reactions is that the first

212 liday junctions are central intermediates in site-specific recombination reactions mediated by tyrosi

213 is the site at which RipX and CodV catalyze site-specific recombination reactions required for norma

214 ubstrates and products of integrase-mediated site-specific recombination reactions results in a singl

215 s lambda and P22 share similarities in their site-specific recombination reactions.

216 ns critically involved in DNA homologous and site-specific recombination, repair, and replication.

217 ase-induced homologous repair we introduce a site-specific recombination signal onto the Y chromosome

218 The dif locus is a site-specific recombination site located within the term

219 In this report, we describe a simple site-specific recombination (SSR) strategy that simultan

220 nts into plants is to utilize one of several site-specific recombination (SSR) systems, such as Cre/

221 Gene targeting and site-specific recombination strategies allow the precise

222 The new work of reveals a new site-specific recombination strategy to establish lysoge

223 e NTE function in vivo, we used the cre/loxP site-specific recombination strategy to generate mice wi

224 gton disease (HD), we have used the Cre/loxP site-specific recombination strategy to inactivate Hdh e

225 such as ColE1, are resolved to monomers by a site-specific recombination system (Xer-cer) whose activ

226 he combination of the Escherichia coli XerCD site-specific recombination system and a protein, FtsK,

227 Circularization of T-DNA by the FLP/FRT site-specific recombination system and/or homologous rec

228 romosome architecture using the phage lambda site-specific recombination system as a probe.

229 We established a conditional site-specific recombination system based on dimerizable

230 A site-specific recombination system catalyses this dimer-

231 Similar experiments with the FLP/FRT site-specific recombination system failed to demonstrate

232 ed the feasibility in Arabidopsis of using a site-specific recombination system FLP/FRT, from the 2 m

233 ort on adapting the P1 bacteriophage CRE-lox site-specific recombination system for the elimination o

234 The Cre/loxP site-specific recombination system has been applied in v

235 The cre-loxP site-specific recombination system has been used success

236 al application of the split intein-regulated site-specific recombination system in building reversibl

237 The placement of the cre/lox site-specific recombination system in many locations in

238 phiRv1 element does indeed encode an active site-specific recombination system in which an integrase

239 The bacteriophage P1 Cre/loxP site-specific recombination system is a useful tool in a

240 e requirements for intasome formation in the site-specific recombination system of bacteriophage HP1.

241 A site-specific recombination system that probes the relat

242 ctivation in plastids that uses the CRE/loxP site-specific recombination system to create a translata

243 eno-associated virus (AAV) with the Cre-loxP site-specific recombination system to selectively knock

244 h tight gpt expression and used the Cre/loxP site-specific recombination system to swap the gpt gene

245 losis prophage-like element phiRv1 encodes a site-specific recombination system utilizing an integras

246 haracterization of plant genes, the Cre-loxP site-specific recombination system was adapted to make r

247 The yeast FLP/FRT site-specific recombination system was used to excise an

248 By using the CRE-lox site-specific recombination system we have deleted clpP1

249 es by making use of the bacteriophage lambda site-specific recombination system.

250 by modules derived from the yeast "Flp/FRT" site-specific recombination system.

251 excision of SCCmec is mediated by an unusual site-specific recombination system.

252 excision with the P1 bacteriophage Cre-loxP site-specific recombination system.

253 Many natural DNA site-specific recombination systems achieve directionali

254 Site-specific recombination systems could allow highly e

255 For nearly 15 years, the use of site-specific recombination systems in plants has focuse

256 ates that, following the use of the Cre/loxP site-specific recombination systems in vivo, it is prude

257 An essential feature of many site-specific recombination systems is their ability to

258 Cre/lox site-specific recombination systems provide important to

259 Although some site-specific recombination systems simply involve bindi

260 hnology can also be used in combination with site-specific recombination systems to facilitate the st

261 By using two site-specific recombination systems, we tied all biologi

262 "combined step" method that makes use of two site-specific recombination systems: one for integrating

263 rsions on attached-XY chromosomes by FLP-FRT site-specific recombination technology followed by irrad

264 avoids embryonic lethality, we used Cre/lox site-specific recombination technology.

265 DivIVA or GFP molecular tag fusions based on site-specific recombination technology.

266 t mediate DNA relaxation, DNA transposition, site-specific recombination, telomere resolution, RNA sp

267 00-fold less proficient in supporting lambda site-specific recombination than wild-type cells.

268 This review focuses on conservative site-specific recombination that generates reversible DN

269 In Cre site-specific recombination, the synaptic intermediate i

270 uption of wobble base pairing of SsrA due to site-specific recombination, thus disrupting the trans-t

271 d recombinational cloning that uses in vitro site-specific recombination to accomplish the directiona

272 Here we used fluorescence microscopy and site-specific recombination to characterize interactions

273 We use piggyBac FLP-FRT site-specific recombination to create deletions and dupl

274 hybrid vector system utilizes Cre-mediated, site-specific recombination to excise an EBV episome fro

275 nked by prophage att sites can be excised by site-specific recombination to generate non-replicating

276 We used Cre/loxP site-specific recombination to genetically measure the m

277 showed that infecting P22 phages can perform site-specific recombination to its maximum efficiency in

278 plasts of the two plants were fused to allow site-specific recombination to join a promoter from toba

279 Bacteriophage lambda uses site-specific recombination to move its DNA into and out

280 Using site-specific recombination to place different construct

281 of a novel technology that utilizes in vitro site-specific recombination to provide a robust and flex

282 ParA mediates site-specific recombination to resolve plasmid multimers

283 rmed by Cre protein and target DNA restricts site-specific recombination to sequences containing dyad

284 ma gondii strain that will permit the use of site-specific recombination to study the host-parasite i

285 in a variety of cellular processes including site-specific recombination, transcription, and DNA repl

286 We propose the term "tRNA-mediated site-specific recombination" (tRNA-SSR) for this mechani

287 nt here a general model for integrase family site-specific recombination using the geometric relation

288 TangleSolve is a program for analysing site-specific recombination using the tangle model.

289 Induced site-specific recombination was also seen when the bis-P

290 Reciprocal and conservative site-specific recombination was observed in 54% of cells

291 Site-specific recombination was used in vivo to produce

292 atory elements in maintenance of repression, site-specific recombination was used to uncouple preasse

293 CAI7 (ade2/ade2), previously constructed by site-specific recombination, was avirulent in immunosupp

294 Using site-specific recombination, we created a nonreplicating

295 Peptide inhibitors of phage lambda site-specific recombination were previously isolated by

296 ised from and integrated into the genome via site-specific recombination, whereas pandemic Aux3 recom

297 ver, expression was restored by Cre-mediated site-specific recombination, which excised the loxP-kanM

298 bilizing completely, P elements will undergo site-specific recombination with the homologous chromoso

299 We found that excision of ICEBs1 occurs by site-specific recombination within 60 bp direct repeats

300 smotic protection (K(+) transport gene), and site-specific recombination (xerD gene).