ライフサイエンスコーパス: solubilization

1 digestion (intestinal lipolysis and micellar solubilization).

2 0 and 15%) which were used for beta-carotene solubilization.

3 SMA variants tested in the various steps of solubilization.

4 n that was efficiently isolated by detergent solubilization.

5 ith membrane proteins are based on detergent solubilization.

6 able number of zwitterion moieties for water solubilization.

7 eral transformations, particularly phosphate solubilization.

8 questers the IMP from water and promotes its solubilization.

9 ation promote the speed and yield of vesicle solubilization.

10 as an alternative to extraction and in vitro solubilization.

11 ion is thought to be important for phosphate solubilization.

12 ut these effects are lost upon peptidoglycan solubilization.

13 y all play distinct roles in the kinetics of solubilization.

14 e ED-2003 applied for the first time for QDs solubilization.

15 perties that typically result from detergent solubilization.

16 tetrameric PSI structure prior to detergent solubilization.

17 into the aqueous solution results in partial solubilization.

18 to explain the actual mechanism involved in solubilization.

19 ein machine is tuned for enzymatic cellulose solubilization.

20 sistently reported to require 2-6 m urea for solubilization.

21 raction network that remains after 1% Triton solubilization.

22 or a better suitability for membrane protein solubilization.

23 tion may explain the observed differences in solubilization.

24 ible endothermic transition at all stages of solubilization.

25 wo-state irreversible model at all stages of solubilization.

26 rhodopsin thermal bleaching at all stages of solubilization.

27 ts role in enzymatic and microbial cellulose solubilization.

28 stinguishable for all bR's, and reflected PM solubilization.

29 roteins that are associated with Axl and Mer solubilization.

30 ally with membranes, requiring detergent for solubilization.

31 tabilized hLHR dimers/oligomers to detergent solubilization.

32 t micelles or liposomes for membrane protein solubilization.

33 forms dimers that can be purified following solubilization.

34 t under 170 degrees C is effective on sludge solubilization.

35 nhanced WAS solubilization, with the highest solubilization (0.16 mg chemical oxygen demand (COD)/mg

36 hemical studies focusing on combination drug solubilization, 3-drug PEG-b-PCL micelles were evaluated

37 ligible volatility at room temperature, high solubilization ability, and tunable selectivity.

38 of multiple genes involved in Pi uptake and solubilization, accumulation of organic acids, enhanced

39 applications as membrane transport and salt solubilization agents and sensors.

40 rged as a powerful tool for membrane protein solubilization and analysis.

41 CD and ethanol as cosolvent on complexation, solubilization and antioxidant activity of caffeic acid.

42 anic solvent for simultaneous sample analyte solubilization and AuNPs stabilization.

43 ing to salt stress acclimatization, nutrient solubilization and competitive root colonization.

44 Carbohydrate solubilization and conversion of unpretreated milled bio

45 on of cyclodextrin-based technology for drug solubilization and delivery.

46 surfactant commonly used to improve protein solubilization and digestion efficiency, can lead to in

47 to the duodenum, where it contributes to the solubilization and digestion of lipid-soluble nutrients.

48 The increased solubilization and elimination of DHT corresponded to sl

49 r systems were first characterized for their solubilization and encapsulation of the drug.

50 The rhamnolipid caused a significant solubilization and enhanced biodegradation of PAHs sorbe

51 Because matrix solubilization and increased wall plasticity have not be

52 This potentially results in cholesterol solubilization and leakage from lysosomes.

53 e membrane, which is well below the onset of solubilization and little dependent on incubation time;

54 L)18 gene), (iv) required pU(L)25 for proper solubilization and localization within the viral replica

55 sing results as a pretreatment, with similar solubilization and markedly better dewaterability levels

56 tably in the direct vicinity of the root via solubilization and microbial activity.

57 e protein complexes as well as by the direct solubilization and nanoparticle incorporation of a viral

58 dhesive restorative systems on growth factor solubilization and odontoblast-like cell differentiation

59 n suffer from low accuracy due to incomplete solubilization and potential volatilization, whilst othe

60 Solubilization and proteolytic activation are necessary

61 in right-side-out membrane vesicles or after solubilization and purification in dodecyl-beta-D-maltop

62 can vary depending on the detergent used for solubilization and purification.

63 he loss of the lipid bilayer during membrane solubilization and purification.

64 Styrene-maleic acid copolymers allow for solubilization and reconstitution of membrane proteins i

65 otocol of frontal cortex processing includes solubilization and reduction/alkylation of proteins in t

66 etic formulation additives preserve the drug solubilization and release characteristics of the parent

67 One such mechanism is the microbial-driven solubilization and remineralization of complex forms of

68 ethod in combination with sequential protein solubilization and shotgun two-dimensional liquid chroma

69 This raises the question of how Hh solubilization and spreading is achieved.

70 ipod amphiphiles are designed to promote the solubilization and stabilization of intrinsic membrane p

71 tin matrix exhibited remarkably low collagen solubilization and sustained the bulk elastic properties

72 Inefficient starch solubilization and the inability to standardize sugar co

73 in (AFABP/aP2) facilitates the intracellular solubilization and trafficking of lipids within the aque

74 actic acid) (PEG-b-PLA) micelles affect drug solubilization, and a paclitaxel (PTX) loaded-PEG-b-PLA

75 and may be necessary for protein extraction, solubilization, and denaturation; however, their presenc

76 , polyethylene glycol (PEG) groups for water solubilization, and either primary amines or biotin grou

77 d in translation, stress protection, protein solubilization, and metabolism.

78 n of elution buffers, detergents for protein solubilization, and stabilizers to maintain complex inte

79 he PM, KRAS undergoes cycles of endocytosis, solubilization, and vesicular transport to the PM.

80 variables: the support medium; gas molecule solubilization; and the catabolic population.

81 mong the variables studied, starch standard, solubilization approach, and wavelength detection affect

82 Type of starch standard, solubilization approach, iodometric reagents, and wavele

83 on tissue retrieval by biopsy and subsequent solubilization, are limited in terms of accurate represe

84 tic formulations that greatly enhance tissue solubilization as well as preserve bioactivity of solubi

85 n mixtures were not amenable to Triton X-100 solubilization at one or more temperatures.

86 PG) showed better association with cell wall solubilization at RT than the enzymes involved in arabin

87 cellulose deconstruction and lignocellulose solubilization at simulation conditions, enabling a high

88 ield of: (a) disintegration degree [DD (%)] (solubilization), (b) filtration constant [F(c) (cm(2)/mi

89 sulfate (SDS) decellularization or stepwise, solubilization-based antigen removal using amidosulfobet

90 is not known how SMA composition affects the solubilization behavior of SMA.

91 Concomitant with matrix solubilization, beta-expansin treatment induced creep, r

92 anced paclitaxel (PTX) to humans due to drug solubilization, biocompatibility, and dose escalation.

93 with ripening appeared not related to pectin solubilization but to weakening of glycan bonding and lo

94 nt, there is a large increase in the overall solubilization, but the nanotubes are suspended as bundl

95 Cellulose solubilization by a Cel48S and Cel48Y double knockout wa

96 ence that CipA plays a key role in cellulose solubilization by C. thermocellum, and it raises interes

97 ted enzymes that could not be refolded after solubilization by denaturation.

98 by differential centrifugation and membrane solubilization by digitonin.

99 est that chaotropes primarily induce protein solubilization by direct binding to concavity in the mol

100 This was achieved after solubilization by dodecylphosphocholine and purification

101 Homogalacturonan solubilization by pectate lyase and calcium chelation gr

102 Interestingly, membrane solubilization by some detergents is relatively slow and

103 We have studied the solubilization by Triton X-100 of binary mixtures compos

104 e aqueous phase, probably by suppressing the solubilization capability of the micelles.

105 s and poly(2-oxazine)s with respect to their solubilization capacity for two extremely water insolubl

106 perature indicated the greater triglycerides solubilization capacity of lecithin based microemulsions

107 It was found that the solubilization capacity of the micellar carrier systems

108 sm, which is significantly stronger than the solubilization caused by the co-solvent effect.

109 ipid biosurfactant is more efficient for the solubilization compared to the synthetic surfactants.

110 of IMPs by diluting them from their initial solubilization condition into different detergents.

111 ing and enrichment by optimizing washing and solubilization conditions and implementing sample fracti

112 ns of two biosensor approaches for screening solubilization conditions for G-protein-coupled receptor

113 ecies are only observed under less favorable solubilization conditions, consistent with aggregation o

114 Once the system melted down, solubilization could occur.

115 rm of plots, that we have called temperature-solubilization diagrams.

116 nd oxidation of Fe in modulating trace metal solubilization, dispersion, and plant uptake.

117 via conventional n-dodecyl-beta-D-maltoside solubilization (DM-PSI) and a, to our knowledge, new det

118 To understand the pathways of iron solubilization during atmospheric transport, we exposed

119 nced SGD altered mercury partitioning and/or solubilization dynamics in coastal waters.

120 contribute further understanding of struvite solubilization, element release and secondary oxalate fo

121 he natural membrane environment by detergent solubilization eliminates most receptor activities, pres

122 s combinations of biochemical techniques for solubilization, enrichment, and immunoprecipitation.

123 become the dominant oligomers after peptide solubilization, even at low (1 muM) concentrations and s

124 bound Rab GTPases and, thus, is not merely a solubilization factor.

125 nfolding is a more important phenomenon than solubilization for increased DNPH labeling.

126 was comparable with that by the conventional solubilization formulation (Taxol(R)), but yielded less

127 sidues of SpoIVFB were required for improved solubilization from membranes by Pro-sigma(K)(1-126) and

128 -126) parts contributed to improving SpoIVFB solubilization from membranes, but only the sigma(K) par

129 ust deposition as well as the extent of iron solubilization from the dust once it enters the ocean.

130 to study the structure of an IMP, detergent solubilization from the membrane is usually the first st

131 loss, resulting in an enhancement of antigen solubilization from the surface of the patch, and increa

132 ta-expansin were negative and polysaccharide solubilization had weak temperature dependence, which in

133 Solubilization has been assayed in the 4-50 degrees C ra

134 The thermodynamic details of solubilization have been extensively investigated, but t

135 n formation followed by selective (Ld phase) solubilization in a model system with a complex biologic

136 e in the study of membrane proteins is their solubilization in a stable and active conformation when

137 geneous, monodisperse, and well-folded after solubilization in Anzergent 3-14 at the analyzed concent

138 anhydride-based amphiphilic polymers for QDs solubilization in aqueous media.

139 The origin of resistance to detergent solubilization in certain membranes, or membrane compone

140 or studying intact membrane proteins involve solubilization in detergent.

141 solated from primary alphabeta T cells after solubilization in digitonin.

142 of bioactives from lipid droplets, and their solubilization in mixed micelles.

143 lated proteins, as measured by resistance to solubilization in nonionic detergent and by copatching w

144 rs to enhance both polarization (via monomer solubilization in surfactant micelles) and adherence of

145 Lower metal solubilization in the bulk soils should translate into r

146 e probability of achieving the required drug solubilization in the colon with the CCSD concept and th

147 of osteoclast-derived MMP-7 to promote RANKL solubilization in the tumor-bone microenvironment was ex

148 By testing lyc concentrations solubilization in tomato seed oil and E:Z ratios of 75:2

149 thesis, stability in the membrane and, after solubilization, in detergents are not well understood fo

150 Unexpectedly, we observed that detergent solubilization induces the formation of fully active Fts

151 Protein solubilization is a key step in mass spectrometry-based

152 ize aggregates in the effluent show that the solubilization is based on a sub-CMC aggregate-formation

153 f action of SMA copolymers in which membrane solubilization is mainly driven by the hydrophobic effec

154 lipids at pH > pHagg, we found that membrane solubilization is promoted by a low charge density and b

155 tor profiles while avoiding crosslinking and solubilization issues.

156 -ionic detergent, Triton X, induced biphasic solubilization kinetics of surface-immobilized vesicles

157 d that sonication, meant to increase protein solubilization, leads to artifacts of metal loss from me

158 to TP_SFE_CO(2) data confirmed that partial solubilization mainly depended on oil availability vs. l

159 We describe a microwave-assisted protein solubilization (MAPS) method to dissolve proteins in rea

160 ) suggest that PuO(2).xH(2)O((am)) reductive solubilization may be important in reducing environments

161 n into the lipid membrane and that efficient solubilization may be improved by a more homogenous dist

162 e most commonly used vortex-assisted protein solubilization method, MAPS reduces the solubilization t

163 in aqueous solution), superior to many other solubilization methods, and provided sustained release (

164 sed as the geometry for 3D citrate-phosphate solubilization models.

165 Aqueous solubilization, necessary for many types of biophysical

166 ees C, thus for some SM-cholesterol mixtures solubilization occurred both above and below 25 degrees

167 ond part, the interplay between the micellar solubilization of (pro)vitamins (beta-carotene or retiny

168 absence of DMRB, EDTA facilitated reductive solubilization of 89% (without AQDS) to 98% (with AQDS)

169 e the fact that all particles are created by solubilization of a common FC/PL membrane domain.

170 Here we report that solubilization of a GPCR, type II cannabinoid receptor C

171 Therefore, the surfactant-induced membrane solubilization of a negatively charged phospholipid (DPh

172 et protein is isolated by homogenization and solubilization of a source (e.g., cell line) followed by

173 The solubilization of A(2A)R showed extraction efficiencies

174 nditions for the overexpression and membrane solubilization of an MsrQ-GFP fusion and set up a purifi

175 hat acidification to pH 2.0 achieved good Zn solubilization of around 70%, but only 3-7% of Cu was be

176 AG-F) showed greater ability in reducing the solubilization of C-terminal cross-linked telopeptide (I

177 xperimental data demonstrating that enhanced solubilization of cellulose can be achieved by the THF-w

178 The CK1 inhibitors (DRB and CK1-7) and solubilization of CK1 restored microtubule sliding in pf

179 be considered when using a protein-assisted solubilization of CNT in water for biomedical applicatio

180 nactive RAB6_T27N mutant led to an increased solubilization of COH1 from lipid membrane preparations.

181 Our results indicate that solubilization of crystalline cellulose by C. thermocell

182 m that CipA is, in fact, necessary for rapid solubilization of crystalline cellulose, the gene was de

183 The comparison of the in vitro data on solubilization of Cu NPs and in vivo data on Cu accumula

184 Attempts were also made to enhance the solubilization of curcumin in aqueous equimolar mixed su

185 o the inner monolayer cause relatively rapid solubilization of detergent-saturated bilayers.

186 e acids (BAs), whose primary function is the solubilization of dietary lipids for digestion and absor

187 We found excellent solubilization of different 3rd generation taxoids irres

188 as other micelle forming detergents, is the solubilization of different membrane proteins.

189 ipid surfactant at sub-CMC concentrations on solubilization of dodecane in porous media under dynamic

190 Herein, enabled by effective solubilization of ECM proteins using our recently develo

191 ApoA-I-mediated spontaneous solubilization of either multilamellar or unilamellar ve

192 per, we report on the successful loading and solubilization of EpoB in a poly(d,l-lactic-co-glycolic

193 cinic acid (EDDS) leads to stabilization and solubilization of Fe at natural pH.

194 tein size and m (FA+pH) We then measured the solubilization of fibrils formed from different FapC var

195 stances (EPS) and the subsequent release and solubilization of fixed metals.

196 Furthermore, UNC119 facilitated solubilization of G(t) from dark-adapted rod OS membrane

197 etreatments should be applied to enhance the solubilization of holocelluloses and increase their furt

198 gressed recently from methods for the simple solubilization of hydrophobic drugs to 'smart' carriers

199 centration and temperature on hydrolysis and solubilization of hydroxypropylated starches were examin

200 ere we devise a general strategy for in vivo solubilization of IMPs in structurally relevant conforma

201 This technique, called SIMPLEx (solubilization of IMPs with high levels of expression),

202 Solubilization of integral membrane proteins in aqueous

203 l-length, monomeric BAK by mutagenesis-based solubilization of its C-terminal alpha-helical surface.

204 own ethers have been used for increasing the solubilization of KF during several decades.

205 describes procedures for (i) the preparation/solubilization of lignin and tannin, (ii) the phosphityl

206 The kinetics of micellar solubilization of lipophilic micronutrients (bioaccessib

207 Azo-aided top-down proteomics enables the solubilization of membrane proteins for comprehensive ch

208 cal research that can offer a detergent-free solubilization of membrane proteins maintaining their na

209 f a sample processing workflow allows entire solubilization of membrane proteins.

210 The solubilization of membranes by detergents is critical fo

211 uitously expressed in all species to aid the solubilization of misfolded proteins, protein degradatio

212 ability of detergents for the extraction and solubilization of Mistic-tagged membrane proteins.

213 ivo evidence for Shh shedding and subsequent solubilization of N-terminal-truncated proteins.

214 less selective but show an enhanced overall solubilization of nanotube material.

215 al yield in the extract, probably due to the solubilization of other substances.

216 he HDL particles produced by apoA-I-mediated solubilization of phospholipid (PL)/free (unesterified)

217 PIP2 also increased the spontaneous solubilization of phospholipid liposomes by apoA1.

218 HDL particles created by either spontaneous solubilization of phospholipid multilamellar vesicles or

219 tenna sizes was achieved with mild detergent solubilization of photosynthetic membranes and fractiona

220 nisms contributing to chemical and enzymatic solubilization of plant cell walls is critical for enabl

221 This study measured reductive solubilization of plutonium(IV) hydrous oxide (Pu(IV)O(2

222 pressurized water extraction showed a high solubilization of protein material up to 95% of inserted

223 n infected cells, Y451A and P452A diminished solubilization of pU(L)6, reduced incorporation of the p

224 In summary, we propose that the solubilization of RANKL by MMP-7 is a potential mechanis

225 and quantifying the surfactant insertion and solubilization of SLBs offers additional insights into t

226 wed that CAPB could dramatically enhance the solubilization of soluble proteins (PN), polysaccharides

227 lead to the solute concentration gradients: solubilization of solutes by micelles and differential a

228 measurement of optical density loss and the solubilization of substrate.

229 ocatalytic saccharification through complete solubilization of the biomass, including the lignin frac

230 Solubilization of the C18 fundamental circuit of alpha-g

231 Solubilization of the Cu NPs applied to the leaves was a

232 ia coli, the substrate dramatically improved solubilization of the enzyme from membranes with mild de

233 Upon dodecylmaltoside solubilization of the membrane fraction, Cx26M34A and Cx

234 erfacial Marangoni flows induced by micellar solubilization of the oil phase.

235 esence of pectin from the fruit hampered the solubilization of the phenolic compounds (with exception

236 The results suggest that solubilization of the polysulfur linkage is a key parame

237 finally, the peptides destabilize and induce solubilization of the supported membranes.

238 d for optimization of the SMA technology for solubilization of the wide variety of cell membranes fou

239 r was used to effect a non-detergent partial solubilization of thylakoids from spinach.

240 Detergent solubilization of thylakoids post cross linking and blue

241 y of lecithin-based microemulsion system for solubilization of triglycerides which can potentially be

242 Dendrimerization as a method of solubilization of UCNPs opens up numerous possibilities

243 ified beta-expansin from maize pollen led to solubilization of wall matrix polysaccharides, dominated

244 e copper binding sites, effects of detergent solubilization on activity and crystal structures, and t

245 entrations of monovalent salt can induce the solubilization or crystallization of NPs and proteins.

246 The approach did not require any protein solubilization or purification and may be a general appr

247 ermine its utility as a pretreatment (sludge solubilization) or postreatment (organic matter removal)

248 esult in composition change, area expansion, solubilization, or the formation of protrusion features

249 served profound synergistic and antagonistic solubilization patterns for the coformulation of the two

250 Studied variables were protein solubilization pH (acid vs. alkaline) and blending step

251 After tissue solubilization, photo-tagged proteins are isolated and i

252 that these changes were due to differential solubilization, photodegradation, evaporation, and/or bi

253 udy was to develop and evaluate a novel drug solubilization platform (so-called solid nanodispersion)

254 ydrotrope at any proportion, show unexpected solubilization power and allow strange but yet unexplain

255 maceutical toxicity profiles, and their high solubilization power of both polar and nonpolar compound

256 ) as sodium salt is commonly used in protein solubilization prior to tryptic digestion, but the prese

257 several advantages, including improved drug solubilization, prolonged circulation, reduced immunogen

258 retinoid-based therapies, including improved solubilization, prolonged circulation, reduced toxicity,

259 number of protein-containing micelles after solubilization, providing the number of reconstituted ch

260 We review examples of solubilization, purification, long-term stabilization of

261 s of a combination of selective Triton X-114 solubilization, radiolabeling with [(3)H]palmitic acid,

262 exhibited a 100-fold reduction in cellulose solubilization rate, although it was eventually able to

263 -I is the main determinant of in vitro lipid solubilization rates, whereas loss of cellular lipid rel

264 mple vial containing proteins suspended in a solubilization reagent is placed inside a domestic micro

265 ability initiate a response resulting in the solubilization, reduction, and uptake of soil iron.

266 CXCL13 solubilization requires the protease cathepsin B that cl

267 oduced the effect that small improvements in solubilization resulted in significant potentiation of t

268 issue culture cells, we performed a polysome solubilization screen and identified conditions that dis

269 ane proteins pertaining to their extraction, solubilization, stabilization, and crystallization, as w

270 , quantification of hydroxyproline (collagen solubilization), static and dynamic nanomechanical analy

271 access the real-time kinetics and elementary solubilization steps of sub-micron sized vesicles, which

272 potato starch standards, and utilize similar solubilization strategies, but had not been comprehensiv

273 t lens studies along with human lens protein solubilization studies were conducted.

274 tion, compared to other conventional protein solubilization techniques.

275 e significant increase in the lower critical solubilization temperature (LCST) of PNIPAM observed upo

276 We performed medium-scale expression and solubilization tests on 351 rationally selected membrane

277 st, 'Karantoki' showed more extensive pectin solubilization than 'Morphitiki'.

278 ups turned out to provide significantly less solubilization than tert-butyl groups in these compounds

279 tein solubilization method, MAPS reduces the solubilization time significantly, increases the amount

280 lipid/detergent 1:20 mol ratio) and extended solubilization times (24-48 h) certain mixtures were not

281 on-degradative epitope recovery and membrane solubilization to enable the detection of a multitude of

282 polymeric ligands for quantum dot (QD) water solubilization to yield biocompatible and derivatizable

283 in detergent micelles, suggesting detergent solubilization traps the protein in a nonactivatable sta

284 ysis, nuclei isolation/washing and chromatin solubilization under mild conditions are bypassed to avo

285 The results hold promise for polysaccharide solubilization under mild conditions, as well as for new

286 erol mixtures were special in that detergent solubilization was accompanied, for certain temperatures

287 o engage in mixed-mode mechanisms for solute solubilization was established.

288 of these processes, we conclude that matrix solubilization was linked closely to changes in wall pla

289 Without AQDS, bioreductive solubilization was slower ( approximately 22 days) and l

290 ein crosslinking followed by ionic detergent solubilization, we show that Hsp93 directly binds to the

291 neral adding high-melting lipids limited the solubilization, whereas the addition of low-melting lipi

292 odegradation of soil-sorbed PAHs by micellar solubilization, which increase the cell exposure to the

293 drogen bonding, polar, and ionic) for solute solubilization while keeping it intact during analyte el

294 After capping with a multilayer shell and solubilization with a multidentate ligand, this class of

295 pe receptor, in the absence of ligand, after solubilization with a variety of detergents.

296 tokes radius of 6.3 nm can be obtained after solubilization with the non-ionic detergent C(12)E(8), f

297 ocedure consisting of lysozyme treatment and solubilization with the nonionic detergent n-dodecyl-bet

298 Cytosolic tau was resistant to solubilization with urea and Triton X-100, indicating th

299 up to 2.13 mg N/L substantially enhanced WAS solubilization, with the highest solubilization (0.16 mg

300 We propose here a general principle for solubilization without conventional surfactants: the bal