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1 rate deuteration, but displays a substantial solvent isotope effect.
2 pe effect of 1,002 +/- 0.012 and the inverse solvent isotope effect.
3 ted by the measurements of large solvent and solvent isotope effects.
4 y-state kinetics and deuterium substrate and solvent isotope effects.
5  and thus contribute to the observed kinetic solvent isotope effects.
6 eters k (cat), K(m) , and k (cat)/K(m) ; and solvent isotope effects.
7 in a RNase are coupled and display identical solvent isotope effects.
8 ofiles of wild-type and mutant ribozymes and solvent isotope effects.
9 bstrate binding was manifested by an inverse solvent isotope effect (0.67 +/- 0.15) on kcat/Km.
10                         From a small, normal solvent isotope effect (1.38 +/- 0.04), it was concluded
11 fects in the L --> D direction and increased solvent isotope effects (1.5-2-fold) but unchanged prima
12                                              Solvent isotope effects accompany both phases and are ex
13                                          The solvent isotope effects also suggest that, at least for
14 e reaction was subject to an inverse kinetic solvent isotope effect analogous to that observed in the
15 s process shows an inverse k(H)/k(D) kinetic solvent isotope effect and involves protonation of a nea
16                               (3) Results of solvent isotope effect and proton inventory experiments
17            Kinetic investigations, including solvent isotope effects and enzyme inactivation by N-eth
18   In addition, the mutants display increased solvent isotope effects and increased levels of steady-s
19  followed the Hofmeister series, analysis of solvent isotope effects and of the changes in LCST with
20  rate of YZ(*) decay exhibited a significant solvent isotope effect, and the rate of recombination an
21  use of dead-end and product inhibition, the solvent isotope effect, and the solvent viscosity effect
22 th measurements of rate/pH profiles, kinetic solvent isotope effects, and ion dependence of GTP hydro
23  pH-dependent NMR chemical exchange, kinetic solvent isotope effects, and mutation, we show that a lo
24 is of protein stability, kinetic parameters, solvent isotope effects, and pH-rate profiles for key Sr
25 a) measurements of the active site cysteine, solvent isotope effects, and solvent viscosity effects,
26 eriments, we use pH-rate profiles, deuterium solvent isotope effects, and solvent viscosity measureme
27                                        (iii) Solvent isotope effects are (D)K(assoc) = 0.5 and (D)k(2
28                                              Solvent isotope effects are observed for both wt and T29
29 ted lactate establishes that the primary and solvent isotope effects arise from the same chemical ste
30 nalysis suggests that future measurements of solvent isotope effects as a function of pressure, in th
31  transfer is suggested by multiple substrate/solvent isotope effects, as well as a proton transfer in
32 ly pH-independent (pH 5-9.5), shows no D(2)O solvent isotope effect at pH 7.7, and decreases with inc
33 (+)] + k(2)' ' and shows a significant D(2)O solvent isotope effect at pH 7.7.
34                                          The solvent isotope effects at the pH maxima are 1.1-1.3, in
35                                          The solvent isotope effects at the pH maxima are 1.3-1.4, in
36 t substrate pro-alpha-factor exhibits a weak solvent isotope effect, but neither this isotope effect
37                                The lack of a solvent isotope effect by D2O and the absence of any cha
38                                The deuterium solvent isotope effects confirm the role of the conforma
39 hese experiments include pH profiles and H/D solvent isotope effects consistent with proton transfer,
40                                          The solvent isotope effect (D)2(O)(k(cat)/K(m))(1) = 1.4 +/-
41 ad, speed at low and intermediate loads, and solvent-isotope effects (D2O versus H2O).
42                                          The solvent isotope effect for fumarate reduction in the wil
43 is significantly different from the reported solvent isotope effect for the hydrolysis of sialyglycos
44                 The observed inverse kinetic solvent isotope effect for the reaction of PhXn(+) (k(ob
45                                The deuterium solvent isotope effect for the second-order rate constan
46 , we determined temperature dependencies and solvent isotope effects for the alpha-chymotrypsin-catal
47                                Substrate and solvent isotope effects for the dehydration reactions ha
48                            Deuterium kinetic solvent isotope effects for the human alpha-thrombin-cat
49                                              Solvent isotope effects for the reactions are k(H(2)O)/k
50 state, a result consistent with experimental solvent isotope effects for this enzyme and with establi
51             Additional experiments involving solvent isotope effects, general base mutational analysi
52  through a process involving a rate-limiting solvent isotope effect, generating conformational change
53 ts protonated form and shows a large kinetic solvent isotope effect (H(2)O/D(2)O) of 5.2 +/- 0.5, ind
54                                   Changes in solvent isotope effects (H(2)O/D(2)O) on (19)F chemical
55                        Primary deuterium and solvent isotope effects have now been used to analyze th
56 alize lyase activity with an unusual inverse solvent isotope effect in an engineered microbial system
57 y isotope effects (1.5-2-fold) but unchanged solvent isotope effects in the L --> D direction and inc
58 anistic studies of FIH have revealed inverse solvent isotope effects in the steady-state rate constan
59                                    (18)O and solvent isotope effects indicate differences in the resp
60                                          The solvent isotope effects indicate that enzyme deacylation
61 roposed mechanisms for both high and low pH, solvent isotope effects indicate that proton transfer st
62                                 The observed solvent isotope effect indicates that proton transfer al
63 ependent of pH and did not involve a kinetic solvent isotope effect, indicating that no proton is inv
64 neral base catalysis: a) the k(H2 O)/k(D2 O) solvent isotope effect is 1.4; b) the rate law (hydrolys
65                                          The solvent isotope effect is indistinguishable from unity,
66                                   Further, a solvent isotope effect is not observed for the mutant en
67                                     A finite solvent isotope effect is observed indicating that proto
68                                      A large solvent isotope effect is observed on k(cat) in the pres
69  similarities to mammalian POPs, however the solvent isotope effect (k(0)/k(1)) was 2.2 at both high
70 is observed using PhCDO, and a large inverse solvent isotope effect (k(D)/k(H) approximately 5.9) is
71 gger) = -9 cal mol(-1) K(-1)) and an inverse solvent isotope effect (k(H(2)O)/k(D(2)O) = 0.62).
72 lso was found to be subject to a substantial solvent isotope effect (kH/kD = 2.7), which is significa
73 solvent effects for 3, including the kinetic solvent isotope effect (KSIE) of 2.18 for methanol, are
74                                      Kinetic solvent isotope effects (KSIEs) for the factor Xa (FXa)-
75         The processes exhibit normal kinetic solvent isotope effects (KSIEs) of 2.0 and 1.8, respecti
76 ated sugar, together with the absence of any solvent isotope effect, lead to the conclusion that hydr
77                       There is no detectable solvent isotope effect (<5%) on any of these measurement
78                                          The solvent isotope effect manifests itself after the precur
79 tments to catalysis accompanying the kinetic solvent isotope effect means that this portion of the pr
80 is of the effects of pH, metal substitution, solvent isotope effects, mutant proteins, and alternativ
81 s proposed as an explanation for the inverse solvent isotope effect observed on V/K(DD-CoA) when eith
82 chloride concentrations with an apparent H/D solvent isotope effect of 0.14 +/- 0.05.
83                             The differential solvent isotope effect of 0.6 is consistent with a direc
84 e extraction of an inverse intrinsic kinetic solvent isotope effect of 1.1 (i.e., (D(2(O)))V/K = 0.9)
85                                          The solvent isotope effect of 1.29 (MeOH/MeOD) for acetyl ch
86 owth phase of OEC photoassembly shows an H/D solvent isotope effect of 1.5 +/- 0.2.
87                                    A kinetic solvent isotope effect of 2.2 indicates that the A245T m
88  hydride transfer and an inverse equilibrium solvent isotope effect of 2.6 (i.e., (D(2(O)))K(s) = 0.4
89 orming G(734)., and the decay exhibits a H/D solvent isotope effect of 3.4, consistent with H-atom tr
90                                      A large solvent isotope effect of approximately 6-8 is also obse
91 ed to zero buffer concentration show a small solvent isotope effect of k(H(2)O)/k(D(2)O) = 1.1, consi
92 sis with a single proton in flight, a normal solvent isotope effect of k(H)/k(D) = 1.5, and when extr
93 d mechanism is implicated through an inverse solvent isotope effect of k(HO(-))/k(DO(-)) = 0.5 and a
94              Finally, at high pH, an inverse solvent isotope effect of k(HO(-))/k(DO(-)) = 0.5 indica
95 e V(max) and the V/K(lactate) values exhibit solvent isotope effects of 1.5.
96                         These values yielded solvent isotope effects of 2 on k(cat) and 0.9 on k(cat)
97             The two phases exhibited kinetic solvent isotope effects of 2.5 and 2.3.
98                                       Normal solvent isotope effects of 4.8 +/- 0.1, 3.1 +/- 0.1, and
99                        The pH-dependence and solvent isotope effects of dealkylation in diastereomeri
100 te-limiting on kcat attenuating the observed solvent isotope effect on beta-lactam formation.
101 e presence of D(2)O revealed a large inverse solvent isotope effect on both inactivation and acetylat
102                             In contrast, the solvent isotope effect on k(c) is 2.4.
103 oyl-L-alanine does not exhibit a significant solvent isotope effect on k(cat) ((H)k/(D)k = 0.96 +/- 0
104 t) decreases for this mutant and the kinetic solvent isotope effect on k(cat), which was 2.0 in WT, i
105                      There is no significant solvent isotope effect on the k(cat)/K(sarc) value.
106 ly deuterated molecules that exploit the H/D solvent isotope effect on the lifetime.
107                 AdoMetDC exhibits an inverse solvent isotope effect on the single-turnover kinetics,
108 e and 3.5 for the flavin domain, whereas the solvent isotope effect on this kinetic parameter is 1.0
109 rium oxide as the solvent, consistent with a solvent isotope effect on V(max) of 2.6 observed in stea
110 e measured for these substrates that reflect solvent isotope effects on hydrogen atom transfers that
111       This interpretation is strengthened by solvent isotope effects on k(2) that are largely tempera
112                                              Solvent isotope effects on k(c)/K(m) for hydrolysis of g
113                     Furthermore, significant solvent isotope effects on k(cat) ((D)k(cat)) for Y345F
114         Proton inventory studies demonstrate solvent isotope effects on k(cat) and k(cat)/K(m)(,PEP).
115                                  Significant solvent isotope effects on kcat (k0/kn approximately 1.6
116                                  The lack of solvent isotope effects on kcat/Kpeptide for both peptid
117                   Here, we show strong water solvent isotope effects on the aryl diazonium reaction w
118  viscosity effects, 18O isotope effects, and solvent isotope effects on the kinetic parameter Vmax/Km
119  system, including unprecedented H(2)O/D(2)O solvent isotope effects on the O(2)(a(1)Delta(g)) format
120                                  The lack of solvent isotope effects on the quantum yields excludes a
121                                Substrate and solvent isotope effects on the transient kinetics of red
122                                              Solvent isotope effects on V and V/K were unity, consist
123                             The large normal solvent isotope effects on V/K ((D)(2)(O)V/K) and V ((D)
124 salt, and does not exhibit a primary kinetic solvent isotope effect over the range of pH and cation c
125 lytic rate, coupling efficiency, and kinetic solvent isotope effect parameters, highlighting an impor
126                                          The solvent isotope effects present in the thermodynamic bin
127                                          The solvent isotope effect results also suggest that with ke
128                                 Furthermore, solvent isotope effects reveal a normal k(H 2O)/ k(D 2O)
129             Analysis of gate-open mutant and solvent isotope effects revealed that substrate gating,
130 e use of dead-end and product inhibition and solvent-isotope effect reveals that both domains catalyz
131 ger solution made up from heavy water, whose solvent isotope effect should reduce the probability, th
132                         pH-rate profiles and solvent isotope effects show that deprotonation of the p
133 re was a significant (k(cat))(H)/(k(cat))(D) solvent isotope effect (SIE) for unphosphorylated Tie2 (
134 strate concentrations, a substantial inverse solvent isotope effect (SIE) is observed on V(max)/K(M)
135                                              Solvent isotope effect (SIE) measurements revealed inver
136             The unusual and dramatic inverse solvent isotope effect (SIE) observed for the Q697E muta
137 rnover over a wide pH-range, with an inverse solvent isotope effect (SIE) of k(cat) observed ((D(2)O)
138                                 Steady-state solvent isotope effect (SIE) studies showed an inverse S
139 rmed by a temperature-dependent, k(cat)/K(M) solvent isotope effect (SIE), which indicated a hydrogen
140 emperature kinetic isotope effects (KIE) and solvent isotope effects (SIE), we demonstrate that both
141                                              Solvent isotope effects (SIEs) on k(cat) are similar in
142                             Strongly inverse solvent isotope effects (SIEs) result from inverse lag t
143                                In this work, solvent isotope effects (SIEs) were used as a direct mec
144 as investigated using steady-state kinetics, solvent isotope effects (SIEs), and competitive oxygen k
145 f a beta-silyl carbocation intermediate, and solvent isotope effect studies indicate that this cation
146                                              Solvent isotope effect studies show that there is no rat
147                    This is proven by kinetic solvent isotope effect studies which show that a primary
148 n-metal-based reactivity, we present kinetic solvent isotope effect studies, variable time normalizat
149                This analysis is augmented by solvent isotope effect studies.
150                           This result, and a solvent isotope effect, suggests that a catalytic step i
151 otope effect at pH < 8 can be explained by a solvent isotope effect that affects the free energy chan
152 is, may yield precise estimates of intrinsic solvent isotope effects that are not fully expressed on
153                                      Kinetic solvent isotope effect values of kH/kD = 1.3 +/- 0.1 (P
154 ng steps in the oxidative half-reaction, the solvent isotope effect, viscosity effect, and O-18 isoto
155                                      A large solvent isotope effect was found, and the pKa value was
156 presence of both divalent metal ions, and no solvent isotope effect was measured on either "burst" ph
157 rs k cat, K m, and k cat/ K m along with the solvent isotope effect were examined for the Fe-type NHa
158 DH (NADD) kinetic isotope effect and the D2O solvent isotope effect were increased in dual-label expe
159 ffect, and the rate of recombination and the solvent isotope effect were pH independent from pH 5.0 t
160                                              Solvent isotope effects were also determined.
161                       Product inhibition and solvent isotope effects were also investigated and could
162        In the high-torque, low-speed regime, solvent isotope effects were found to be small; in the l
163                                              Solvent isotope effects were measured during steady-sate
164                                      Similar solvent isotope effects were observed in the pre-steady-
165                 In the current study, pH and solvent isotope effects were utilized to probe the mecha
166 peroxide intermediate and an inverse kinetic solvent isotope effect, which indicates that this proton
167                           Measurement of the solvent isotope effect with deuterated lactate establish
168 ined and show a significantly larger kinetic solvent isotope effect, with an overall magnitude of 10

 
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