ライフサイエンスコーパス: somitic

1 the presomitic mesoderm, we suggest that the somitic aberrations are founded in the disruption of the

2 conserved transcription factors involved in somitic and cardiac mesoderm development in diverse bila

3 ral to the chordamesoderm, embryoids display somitic and intermediate mesoderm, with beating cardiac

4 re we determine the relative contribution of somitic and lateral plate mesoderm to the avian scapula

5 visualization of the cryptic border between somitic and lateral plate populations reveals the dynami

6 the large expanse of superficial presumptive somitic and lateral-ventral mesoderm that initially sepa

7 e ectopically expressed noggin in developing somitic and limb bud mesoderm and observed phenotypes co

8 a component of the splenius muscle, of mixed somitic and nonsomitic origin.

9 Time-lapse recordings show that presumptive somitic and notochordal cells move out of the roof of th

10 ctivity alone is sufficient for lateral fast somitic and pectoral fin fibre formation from the latera

11 zebrafish myod gene delays and reduces early somitic and pectoral fin myogenesis, reduces miR-206 exp

12 Nearby somitic and prechordal mesoderm becomes recruited into t

13 Using microdissected embryonic tissue from somitic and presomitic mesodermal tissue, we identified

14 othelial cells that developed from migratory somitic angioblasts, and assembly of these vessels is li

15 and limb vessels are recognized by mammalian somitic angioblasts.

16 r in the limb bud, and in the development of somitic borders.

17 t of stripes that corresponds to prospective somitic boundaries, suggesting that Notch signaling with

18 x3ILZ/ILZ mutants, which do not form ventral somitic buds.

19 eral plate mesoderm, together act to pattern somitic cell fate.

20 on of the embryo or for the establishment of somitic cell lineages.

21 : the dorsal compartment, formed from purely somitic cell populations; and the ventral compartment co

22 nitors (NMPs) as they commit into neural and somitic cell types.

23 /VASP, alpha5beta1-integrins or talin in the somitic cells abolished the FN pillars, indicating that

24 genic expression of lh3 in a small subset of somitic cells adjacent to where neural crest cells switc

25 ate, in migrating neural crest cells, and in somitic cells along the migratory pathway.

26 icken embryos were generated that consist of somitic cells carrying transgenes expressing one of the

27 ecies, the superficially derived presumptive somitic cells come to lie in the medial region of the pr

28 ed that Shh induces a factor(s) that renders somitic cells competent to chondrify in response to subs

29 In contrast, migrating lateral somitic cells crossing the somitic frontier do not maint

30 In zebrafish, most somitic cells give rise to long muscle fibers that are a

31 t revealing a very different distribution of somitic cells in the scapula than previously reported.

32 Grafted somitic cells in the vicinity of the neural tube maintai

33 occurs suggests that superficial presumptive somitic cells in X. laevis ingress into the deep region

34 By contrast, ectopic somitic cells located distal to the neural tube and in t

35 ion impairs FN matrix assembly, spreading of somitic cells on FN and autophosphorylation of FAK, sugg

36 In contrast to somitic cells that express Lbx1, lateral rectus precurso

37 ion is required to prevent overcommitment of somitic cells to a myogenic fate.

38 PKA (dnPKA) in wild-type embryos causes all somitic cells to develop into slow muscle fibers.

39 uction of Nkx3.2 maintains the competence of somitic cells to initiate chondrogenesis in response to

40 to reveal the precise contributions made by somitic cells to the ribs and associated tissues of the

41 and quantified the contribution of embryonic somitic cells to these progenitor populations.

42 s did not migrate into the branchial arches, somitic cells were capable of migrating and were incorpo

43 Chick somitic cells were labeled by using replication-defectiv

44 n a region known to give rise to prospective somitic cells, and following their movement as they unde

45 ke manner to elicit differing responses from somitic cells, and that Pax3 and Nkx3.2 set up mutually

46 demonstrate that Shh is a potent mitogen for somitic cells, supporting the idea that it may serve to

47 phore patterning depends upon the underlying somitic cells, the myotomal derivatives of which--both s

48 a6b resulted in abnormal somite shape, fewer somitic cells, weaker or absent myf5 expression, and red

49 ntrast, in the lateral myotome and migratory somitic cells, which require the expression of MyoD, exp

50 the specification and migratory behaviour of somitic cells.

51 lls but not in the ones containing wild-type somitic cells.

52 cle fibers, which constitute the majority of somitic cells.

53 hh signaling elicit differing responses from somitic cells: the lowest level of Shh signaling allows

54 al motor axons make incorrect decisions at a somitic choice point.

55 hh signals are required only transiently for somitic chondrogenesis in vitro, and act to provide a wi

56 chondrocytes, a mechanism resembling in vivo somitic chondrogenesis that is not recapitulated with TG

57 ns as a transcriptional repressor to promote somitic chondrogenesis.

58 Nkx3.2 is critical for this factor to induce somitic chondrogenesis.

59 tch/Delta pathway components and oscillating somitic clock genes that are thought to control segmenta

60 The notochord runs along the length of the somitic compartment and is flanked on either side by som

61 o play a role in mechanically elongating the somitic compartment.

62 pression in ventro-caudal and ventro-rostral somitic compartments in the mouse embryo.

63 cle precursors can be attributed to distinct somitic compartments which are laid down prior to overt

64 Two earlier-formed somitic compartments, the sclerotome and myotome, intera

65 spatial regularity of anterior and posterior somitic compartments.

66 Second, the segmentation of the somitic component of the blade is partially lost; and th

67 ian scapula is lateral plate derived and the somitic contribution to the scapular blade is significan

68 As expected, neural crest and some somitic derivatives are identified.

69 the embryonic neural tube, neural crest, and somitic derivatives, contains two DNA-binding domains, a

70 e embryonic somites based on fate mapping of somitic derivatives.

71 lie initially in the lateral portion of the somitic dermomyotome and subsequently migrate to their t

72 on view of the molecular genetics underlying somitic development in mammals.

73 ate and cardiac mesoderm and favored instead somitic differentiation.

74 Somitic endothelial precursors are uncommitted to formin

75 Within the somitic environment, Hh signals restrict hypaxial develo

76 Neither the promoter nor region B can direct somitic expression independently, indicating that the in

77 Somitic expression occurs in the earliest myoblasts that

78 It is therefore possible that the somitic expression of cEbf1 is controlled by Shh signals

79 nts confirmed our hypothesis that the medial somitic expression of cEbf1 is regulated by Shh from the

80 Forced expression of Nkx3.2 blocks somitic expression of the dermomyotomal marker Pax3 both

81 anonical signalling by Wnt16 is required for somitic expression of the Notch ligands deltaC (dlc) and

82 or Wnt and Sonic hedgehog signals can induce somitic expression of the paired box transcription facto

83 Here, we demonstrate that somitic expression of the transcription factor Nkx3.2 is

84 iously demonstrated that Wnt16 regulates the somitic expression of two Notch ligands, deltaC (dlc) an

85 the primitive mechanism of direct epithelial somitic extensions to derive the muscles of the fin.

86 reserve the composition and character of the somitic extracellular matrix containing fibronectin, fib

87 Somitic Fgf10 expression, which is required for normal m

88 and hypomorphic Fgf10 mutants, in which the somitic Fgf10 gradient is shortened dorsally and less ov

89 We propose that the intra-somitic Fgf10 gradient, together with ventral elongation

90 r of mutants with different perturbations of somitic Fgf10 gradients for the presence of WNT signals

91 To test whether somitic FGF10 is required for the formation of these two

92 We correlate increasing levels of somitic FGF10 with progressive maturation of the surface

93 e ectoderm, and show that full expression of somitic Fgf10, co-regulated by GLI3, is required for the

94 migrating lateral somitic cells crossing the somitic frontier do not maintain donor Hox expression bu

95 these anatomical compartments is termed the somitic frontier.

96 the choice point in approximately 40% of the somitic hemisegments and an approximately 150% increase

97 erfaces of cranial and spinal nerves, and in somitic/intersomitic regions along the presumptive spina

98 In all three species, somitic, lateral, and ventral mesoderm move into the dee

99 sue does not form somites, yet expresses the somitic markers Lbx1, Pax7 and Paraxis in a regionalised

100 the neural tube maintained expression of the somitic markers Pax3, MyoD and Pax1.

101 but show no ectopic or enhanced notochord or somitic markers.

102 sgene activity to the heart and the tail pre-somitic mesenchyme.

103 sis as a product of morphogenesis of the pre-somitic mesoderm (PSM) and the periodicity of a molecula

104 enesis, epithelial somites form from the pre-somitic mesoderm (PSM) in a periodic manner.

105 vefronts have now been identified in the pre-somitic mesoderm (PSM), there is not yet conclusive evid

106 ntrolateral lip of wing-level somites or pre-somitic mesoderm (segmental plate), myotome development

107 X-ADAM 13 is localized to somitic mesoderm and cranial neural crest cells during g

108 which direct translation of pattern from the somitic mesoderm and de novo cell and tissue interaction

109 While adam13 is expressed in the somitic mesoderm and in neural crest cells, but not in a

110 ctivation and inhibition of apoptosis in the somitic mesoderm and in repression of Pax7 during neural

111 ntisense oligonucleotides that entirely lack somitic mesoderm and in somite segmentation mutants that

112 During later stages, most of the somitic mesoderm and neural tissue circumscribe the dors

113 een widely separated germ layers, namely the somitic mesoderm and the endoderm, in quail embryos.

114 ans; instead, their basal ends adhere to the somitic mesoderm as a coherent layer, taking on somitic

115 cked somitic subtypes, CDX2 was required for somitic mesoderm but blocked cardiac differentiation.

116 ral fate, then further patterned to generate somitic mesoderm by signals from the gastrula organizer.

117 orm normally in tor mutant embryos, although somitic mesoderm defects are apparent later, when cells

118 continues, and the anterior notochordal and somitic mesoderm differentiate in the pre-involution mar

119 ion marginal zone, posterior notochordal and somitic mesoderm do not differentiate.

120 ere loss of sclerotomal gene expression, and somitic mesoderm from these embryos cannot activate scle

121 In order to test whether segmental plate or somitic mesoderm has the ability to migrate in a cranial

122 e the chondrocyte differentiation program in somitic mesoderm in a bone morphogenetic protein (BMP)-d

123 vertebrates, the dorsoventral patterning of somitic mesoderm is controlled by factors expressed in a

124 al alternating rostral-caudal pattern of the somitic mesoderm is disrupted, suggesting that intersomi

125 Dorsoventral polarity of the somitic mesoderm is established by competitive signals o

126 issues from these explants, we find paraxial somitic mesoderm is nearly twice as stiff as either the

127 Thus, the ;Hox code' in somitic mesoderm is the result of the distinct, graded e

128 very of an activated form of beta-catenin to somitic mesoderm mimics the effects of Wnt-1, demonstrat

129 mechanisms for fin development originated in somitic mesoderm of early vertebrates, and that the orig

130 of molecular clocks and gradients in the pre-somitic mesoderm of numerous vertebrate species has adde

131 Comparing the notochord and adjacent somitic mesoderm reveals that extension can be regulated

132 gnized by mammalian angioblasts derived from somitic mesoderm through analysis of mouse-avian chimera

133 r, Wnt-1 appears to be unable to convert all somitic mesoderm to a dermomyotomal fate.

134 streak, resulting in a lateral expansion of somitic mesoderm to form multiple columns.

135 at key to this interaction is the ability of somitic mesoderm to repress Fgf8 transcription in the pr

136 mary and secondary myotubes are derived from somitic mesoderm, a presumption, which up until now, has

137 nstrating that Wnt-1 likely acts directly on somitic mesoderm, and not through adjacent tissues via a

138 es expression of cartilage-specific genes in somitic mesoderm, but also promotes the proliferation an

139 ting of the deep neural plate and underlying somitic mesoderm, but lacking a midline, show bipolar, m

140 Pax3 is a transcription factor expressed in somitic mesoderm, dorsal neural tube and pre-migratory n

141 he hatching gland, head mesoderm, notochord, somitic mesoderm, endoderm and ventral aspect of the neu

142 As a result, the derivatives of the somitic mesoderm, especially the axial skeleton, are sev

143 in spadetail mutants that have very reduced somitic mesoderm, in no tail mutants injected with spade

144 e the chondrocyte differentiation program in somitic mesoderm, indicating that DNA binding by Nkx3.2

145 hesis in the embryo, particularly within the somitic mesoderm, lateral mesoderm, kidney, heart, and s

146 e axial skeleton, which develops solely from somitic mesoderm, patterning of the rib cage is complica

147 an, in turn, induce robust chondrogenesis in somitic mesoderm, provided that BMP signals are present.

148 flanked laterally by superficial presumptive somitic mesoderm, the anterior tip of which also appears

149 tially from the area of Hensen's node to the somitic mesoderm, the intermediate mesoderm, and then to

150 tially from the area of Hensen's node to the somitic mesoderm, the intermediate mesoderm, and then to

151 rder in axolotl is congruent between LPM and somitic mesoderm, unlike in chicken and possibly other a

152 abeled cells from the prospective notochord, somitic mesoderm, ventrolateral mesoderm, neural ectoder

153 mesoderm, but not older Cerr1-nonexpressing somitic mesoderm, was able to mimic the anterior neurali

154 The thoracic skeleton is derived from both somitic mesoderm, which forms the vertebral bodies and r

155 neural crest-derived structures, as well as somitic mesoderm-derived structures.

156 r role in patterning the dermomyotome of the somitic mesoderm.

157 gene expression patterns observed in the pre-somitic mesoderm.

158 is by derepressing the expression of Sox9 in somitic mesoderm.

159 repressed by such signals and accelerated by somitic mesoderm.

160 ploys SFRP2 to reduce WNT1/4 activity in the somitic mesoderm.

161 and notochord promote chondrogenesis of the somitic mesoderm.

162 ions and that this effect is mediated by the somitic mesoderm.

163 or condensation and/or epithelization of the somitic mesoderm.

164 ression was limited to the lateral plate and somitic mesoderm.

165 m formation including absence of most of the somitic mesoderm.

166 de and appears to pre-configure the emerging somitic mesoderm.

167 eganglionic projections is influenced by the somitic mesoderm.

168 nes involved in specification of ventral and somitic mesoderm.

169 quires guidance cues located in the adjacent somitic mesoderm.

170 tissues, like the neural crest (NC) and pre-somitic mesoderm.

171 rives dynamic gene expression within the pre-somitic mesoderm.

172 or tendon arise from either neural crest or somitic mesoderm.

173 rtilage and tendon lineages arising from the somitic mesoderm.

174 itic mesoderm as a coherent layer, taking on somitic morphology while still a part of the archenteron

175 t perlecan is essential for the integrity of somitic muscle and developmental angiogenesis and that e

176 rally located secondary tail containing both somitic muscle and notochord.

177 ed locus with enhancer activity in zebrafish somitic muscle and spinal cord, an activity that is abol

178 r for those cells to induce the formation of somitic muscle and the pronephros.

179 ng heart, while high levels of expression in somitic muscle are maintained.

180 at early stages, but exacerbates the loss of somitic muscle caused by lack of Myod.

181 king planar cell polarity (PCP) signaling in somitic muscle cells also results in non-segmental neura

182 led to strong deficits in notochord but not somitic muscle development.

183 cdk5 kinase activity is required for normal somitic muscle development; expression of cdk5 DN result

184 attern, we ectopically overexpressed GDNF in somitic muscle during the period of motor axon outgrowth

185 ce the formation of posterior structures and somitic muscle in neighboring cells.

186 The patterning of vertebrate somitic muscle is regulated by signals from neighboring

187 pression of cdk5 DN results in disruption of somitic muscle patterning, accompanied by stunting of th

188 s differs from the marker sets indicative of somitic muscle precursors.

189 euron while GDNF is expressed in the ventral somitic muscle that it innervates.

190 lly derived tissues such as neurectoderm and somitic muscle to develop.

191 te progeny to the neural tube, notochord and somitic muscle, as well as other identified cell types w

192 pleural cavities before the migration of the somitic myoblasts.

193 ratogenic doses of DI at the early stages of somitic myogenesis (embryonic day 8.5) exhibited an incr

194 Here, we show that somitic myogenesis is under negative regulation as well;

195 ube, notochord, and surface ectoderm promote somitic myogenesis.

196 erm and identify Pax-3 as a key regulator of somitic myogenesis.

197 Pax3 and Pax7 are expressed by somitic myogenic progenitors and are critical myogenic d

198 to form in vertebrate embryos appear in the somitic myotome.

199 at govern postimplantation expression in the somitic myotomes and the limb bud AER.

200 expressed in dorsal neural tube, can induce somitic Noggin expression.

201 We previously reported that the somitic Notch ligands, Dlc and Dld, are essential for HS

202 at somatic mutations in a progenitor cell of somitic origin may act on a background of germline haplo

203 We show that this restriction to the somitic paraxial mesoderm correlates well with the abili

204 erm, remaining excluded from head, heart and somitic paraxial mesoderm territories.

205 early mesoderm mainly at the expense of the somitic paraxial mesoderm.

206 ow that median fins arise predominantly from somitic (paraxial) mesoderm, whereas paired appendages d

207 dermomyotomal population of cells defined by somitic pax3/7 expression.

208 ucleotides (MOs) phenocopies the b567 mutant somitic phenotype, indicating that her1 and her7 are nec

209 endothelial cells are derived from a common somitic precursor.

210 henotype is due to the fact that some of the somitic precursors are not able to leave the tailbud or

211 , and the periodicity of their expression in somitic precursors mirrors that of Notch signaling-relat

212 ion and BMP inhibition are both required for somitic precursors to exit the tailbud.

213 Within somitic precursors, HRT1 and HRT3 exhibited dynamic expr

214 on assays demonstrated that Cerr1-expressing somitic-presomitic mesoderm, but not older Cerr1-nonexpr

215 hypaxial signals places En1 into the epaxial somitic programme.

216 n of the receptor tyrosine kinase met within somitic regions fated to give rise to appendicular muscl

217 It has remained unclear, however, how these somitic requirements are connected to the later emergenc

218 rest cells entering the rostral half of each somitic sclerotome but avoiding the caudal half.

219 The somitic shape changes cause the adjacent notochord to se

220 was assessed by perturbing its expression in somitic stage chick embryos using a Paraxis-specific ant

221 s showed phenotypes involving alterations in somitic structure and muscle fiber organization, as well

222 phenotypic severity involving alterations in somitic structure and muscle fibre organization as well

223 atenin-expressing cluster can give rise to a somitic structure.

224 ral mechanism for the specification of other somitic subcompartments.

225 How this key somitic subdivision is established remains poorly unders

226 quired for cardiac specification but blocked somitic subtypes, CDX2 was required for somitic mesoderm

227 uced mesoderm patterned into cardiac but not somitic subtypes.

228 supporting its implication in the control of somitic symmetry.

229 cx), a bHLH transcription factor, marks this somitic tendon progenitor population at its inception, a

230 e somite, and propose that the domain of the somitic tendon progenitors is regulated both by the rest

231 ganized streams before migrating through the somitic territory.

232 Misexpression of Nkx3.2 in somitic tissue confers a prochondrogenic response to BMP

233 chord and floor plate confer a competence in somitic tissue for subsequent BMP signals to induce chon

234 by local inductive signals, suggesting that somitic tissue may be fixed at an early point in their d

235 We propose a model in which signalling from somitic tissue promotes the differentiation of the spina

236 Somitic tissue transplanted between axial levels retains

237 raxial mesoderm differentiated normally into somitic tissue, but failed to segment properly.

238 sion of the transcription factor Sox9 in the somitic tissue.

239 and concomitant loss of pronephric duct and somitic tissue.

240 al role in patterning cell fates in adjacent somitic tissue.

241 d endothelial gene expression, and a loss of somitic tissue.

242 Our findings suggest that chondrogenesis of somitic tissues can be divided into two separate phases:

243 Integrated analysis of the somitic transcriptional and chromatin activities identif

244 pression of endogenous E2 activity increased somitic VEGF expression and vascular target regulation,