ライフサイエンスコーパス: spacer

1 nterspersed with short, variable sequences ('spacers').

2 g the length of promoter non-optimal -35/-10 spacer.

3 mido)-hexaethyleneglycol] ester (SM(PEG)(6)) spacer.

4 ome oxidase gene subunit II - the intergenic spacer.

5 uid cultures, most bacteria acquire a single spacer.

6 or without (1) an extended, self-immolative spacer.

7 be improved by the use of an electrophoretic spacer.

8 ce and with no need of the long and flexible spacer.

9 to the grid via a polyethylene glycol (PEG) spacer.

10 orphologies that contain cells with multiple spacers.

11 ciation (GBA), and CRISPR-Cas self-targeting spacers.

12 er mechanism that favors shorter repeats and spacers.

13 rnating repeat sequences and foreign-derived spacers.

14 mbly junctions within the trimmed portion of spacers.

15 n DNA acquired from these invaders, known as spacers.

16 and have unusually short CRISPR repeats and spacers.

17 ensures optimal immunity from newly acquired spacers.

18 es flanking less well-conserved 12- or 23-bp spacers.

19 when employing a racemic mixture of organic spacers.

20 carboxyl-decorated tetraphenylethylene (TPE) spacers.

21 he cassettes contain several dozen expressed spacers.

22 Internal Transcribed Spacer 1 (ITS1) amplicons were sequenced by Illumina MiS

23 Genotyping based on internal transcribed spacer 1 (ITS1) and ITS2 of the rRNA operon has played a

24 ra encountered in prior internal transcribed spacer 1 (ITS1)-based surveys.

25 se FAWs on the basis of internal transcribed spacer 1 (ITS1).

26 sectional study, we use internal transcribed spacer 1 amplicon sequencing to evaluate the salivary my

27 of plasmid and bacteriophage origin known as spacers(1-3).

28 The internal transcribed spacer 2 (ITS2) region of the fungal ribosomal repeat wa

29 loci such as 5.8S rRNA, Internal Transcribed Spacer 2, and 28S rRNA regions, which are commonly used

30 ine, benzimidazole, piperidine) and alkylene spacers (2 to 10 methylene groups between carbamate and

31 d/or 28S-internal transcribed ribosomal gene spacer (28S-ITS) fungal PCR.

32 anded casposon end binding resembles that of spacer 3'-overhangs.

33 actors, including the choice of single-chain spacer(6) and extracellular(7) and costimulatory domains

34 ses that could escape targeting by the first spacer acquired.

35 Thus, spacers acquired during inter-species mating could limit

36 Surprisingly, many of the spacers acquired following inter-species mating target s

37 Inter-species mating induces increased spacer acquisition and may result in interactions betwee

38 nding of the molecular mechanisms underlying spacer acquisition and that have revealed a fundamental

39 Furthermore, we observed naive spacer acquisition at a low level in WT Haloferax cells

40 The rate of spacer acquisition depended on sequence elements within

41 eviously acquired spacers promote additional spacer acquisition from the vicinity of the target DNA s

42 We studied Type III spacer acquisition in phage-infected Thermus thermophilu

43 reign transcripts, most contain conventional spacer acquisition machinery found in DNA-targeting syst

44 Here, we utilize the CRISPR-Cas spacer acquisition process to detect DNA acquisition eve

45 RISPR-Cas immune response and correlate with spacer acquisition rates, but not with spacer targeting

46 ich can respond with both single or multiple spacer acquisition schemes to solve challenges presented

47 CD from bacteriophage destruction and CRISPR spacer acquisition to constructive host DNA repair.

48 Our findings have implications for CRISPR spacer acquisition, off-target potential of CRISPR gene

49 rall, our study provides a complete model of spacer acquisition, which can be harnessed for DNA-based

50 se of the CRISPR-Cas immune response: primed spacer acquisition.

51 new spacers leading to high overall level of spacer acquisition.

52 oli host 3'-5' exonucleases DnaQ and ExoT in spacer adaptation and reveal a mechanism by which spacer

53 The polarized nature of spacer adaptation revealed the historical contingency in

54 During spacer adaptation, the Cas1-Cas2 complex selects fragmen

55 patients managed with retained "destination spacers" after the resection of infected joint prosthese

56 viruses present in the same population carry spacers against each other.

57 s using fungal-specific internal transcribed spacer amplicon sequencing of fecal samples.

58 ylum Bacteroidetes as demonstrated by CRISPR spacer analysis and by analysis of genes acquired by pha

59 A monolithically integrated optical spacer and a reflector further boost the enhancement.

60 pecific for the target, followed by a carbon spacer and a sequence specifically designed not to bind

61 oreactivity increased with the length of the spacer and correlated well with the rate constants estim

62 Spacer and repeat profiles agree qualitatively with thos

63 odeling approach, Ni/Ni/Ni/PMN-PT with Cu as spacer and Terfenol-D/CoFeB/Ni/PMN-PT.

64 al viruses carry mini-CRISPR arrays with 1-2 spacers and preceded by leader sequences but devoid of c

65 idual CRISPR RNAs that each include a single spacer, and direct Cas proteins to complementary sequenc

66 apturing short invader DNA sequences, termed spacers, and incorporating them into the CRISPR loci of

67 termed prespacers, into the CRISPR array as spacers, and interference, the targeted degradation of D

68 ers that conform to a so-called stickers-and-spacers architecture.

69 mulations of model proteins with sticker-and-spacer architectures shed light on the formation of biom

70 During the immunization step, new spacers are acquired by the CRISPR machinery, but the mo

71 be coupled with primed adaptation, in which spacers are acquired from DNA surrounding the targeted p

72 strain is exposed to a microbial sample and spacers are acquired from transferred plasmids and perma

73 ngle site, we demonstrate that inter-species spacers are common.

74 ax volcanii and Haloferax mediterranei, that spacers are indeed acquired chromosome-wide, although a

75 Spacers are interspersed between stickers and their pref

76 Spacers are transcribed and the resulting CRISPR (cr)RNA

77 Next, during the targeting phase, spacers are transcribed into CRISPR RNAs (crRNAs) that g

78 Spacers are transcribed into guide RNAs that direct the

79 Spacers are transcribed into small RNA molecules that gu

80 ort pieces of the invader's genome, known as spacers, are captured and integrated into the CRISPR loc

81 rt sequences from the phage genome, known as spacers, are inserted between the CRISPR repeats.

82 These sequences, known as spacers, are transcribed and processed into small CRISPR

83 e (DSSO) to better understand the effects of spacer arm modulation on MS-cleavability, fragmentation

84 ntain cassettes of viral DNA elements called spacers as a memory of past infections.

85 fore, CRISPR immune cells acquire additional spacers at the same time as they destroy the infecting v

86 to gold nanoparticles (AuNPs) using a thiol spacer attached to 5' end of the hpDNA.

87 ollowed by 16S gene and internal transcribed spacer-based microbiota sequencing.

88 demonstrate that incorporating a nonadhesive spacer between (KY) repeats can mimic the hidden length

89 The spacer between 5S and 18S rRNA genes, especially the reg

90 equence repeats to the docking strand, and a spacer between the docking strand and the affinity agent

91 ticle spacing through changes to the polymer spacer between the hydrogen bonding groups and the nanop

92 Here, we show that the length of the spacer between the SD sequence and P-site codon strongly

93 vations suggest that during translation, the spacer between the SD sequence and P-site codon undergoe

94 ination rate with the introduction of an hBN spacer between WSe(2) and MoS(2), Auger-type and exciton

95 exes (TTCs) containing different-length mRNA spacers between RNAP and the ribosome active-center P si

96 netration, while the use of optimized-length spacers between the Cu-chelating group and the Abeta-int

97 e sites, alleviating the need for dielectric spacers between the metal and 2DC layer.

98 ional and taxonomic profiles for 2.9 million spacers by aligning the spacer content to each sample's

99 zone functionalized with a C6 alkyl thiolate spacer (C6 HAT) was characterized on a number of metal s

100 ve been conducted, showing that the thin GaN spacer can effectively balance the hole concentration be

101 at single-bp changes, including in the 12RSS spacer, can significantly and selectively alter PC forma

102 PR machinery, but the molecular mechanism of spacer capture remains enigmatic.

103 s viruses carrying protospacers matching the spacers catalogued in the CRISPR arrays.

104 Here we demonstrate that the selection of spacer cations (i.e., selective fluorination of phenethy

105 so far proven inaccessible using traditional spacer cations due to either intrinsic instability or fo

106 work provides key missing information on how spacer cations exert influence on desirable electronic p

107 s are formed by the incorporation of organic spacer cations into three-dimensional (3D) double perovs

108 rangements and orientational disorder of the spacer cations result in orientational degeneracy and di

109 ferred molecular configuration of the chiral spacer cations, R-(+)- or S-(-)-1-(1-naphthyl)ethylammon

110 osts a second nucleobase at Calpha through a spacer chain with a triazole linker.

111 n the ratio of molar fluxes and the ratio of spacer channel ion concentrations, regardless of operati

112 hallower dependence on the number of proline spacers compared to the analogous electron transfer (ET)

113 perform deep sequencing of the CRISPRome-all spacers contained in a microbiome-associated with hypert

114 es during reimplantation was 9.5% for cement spacers containing a glycopeptide (27/284) (with or with

115 nd the transcripts are processed to generate spacer-containing mature CRISPR-RNAs that are employed a

116 iles for 2.9 million spacers by aligning the spacer content to each sample's metagenome and correspon

117 des according to cas sequence similarity and spacer content.

118 her appropriate changes in the nature of the spacer could modulate the intramolecular BP/Thy photorea

119 Using multi-spacer CRISPR arrays, we achieved simultaneous multiplex

120 s based on 16S rDNA and internal transcribed spacer deep sequencing.

121 We have designed and used four different spacers, denoted A-D, to connect two pentacenes and to p

122 which does not cleave proteoglycans, caused spacer-dependent reductions in versicanase activities.

123 The arrays containing spacers derived from foreign DNA are transcribed, and th

124 Acquiring foreign spacer DNA into the CRISPR locus is an essential primary

125 binding VWF, the ADAMTS13 cysteine-rich and spacer domain exosites bring enzyme and substrate into p

126 rk on ADAMTS-13 our results suggest that the spacer domain hypervariable loops may exercise significa

127 bstitution of two hypervariable loops in the spacer domain of ADAMTS-5 (residues 739-744 and 837-844)

128 Deletion of the spacer domain reduced versicanase activity of ADAMTS-5 1

129 9-Val-730 and Glu-732-Ala-733 in the ADAMTS7 Spacer domain, which was corroborated by N-terminal sequ

130 tion was reported for the acquisition of new spacers during CRISPR adaptation, and prokaryotic genome

131 us subtilis in a forward osmosis system with spacers during the first 4 h of biofilm formation.

132 eats-CRISPR-associated proteins (CRISPR-Cas) spacers elucidated lineage-specific virus-host dynamics

133 Sequencing and analysis of acquired spacers enables identification of the transferred plasmi

134 The spacer-encoded self-assembly process was investigated by

135 cts derived from the 5'-external transcribed spacer (ETS) of 45S pre-rRNA, as MTR4 does.

136 hanism ensuring acquisition of RNA-targeting spacers exists.

137 e 1 in rats, the introduction of a methylene spacer facilitated prodrug activation, but parent exposu

138 This allows to create a Si(111)|organic-spacer|Fc hybrid interface, where the ferrocene moiety i

139 id structures known as R-loops at intergenic spacers flanking nucleolar rRNA genes.

140 a 3-(aminomethyl)piperidinium (3AMP) organic spacer for the fabrication of mixed Pb/Sn-based perovski

141 single sampling site dwarf the diversity of spacers from all available Sulfolobales isolates and dis

142 ms encode a reverse transcriptase to acquire spacers from foreign transcripts, most contain conventio

143 o <<primed adaptation>> - acquisition of new spacers from in cis located sequences.

144 tally investigate the properties of galfenol/spacer/galfenol structures in which the compositions of

145 The phenylene spacer groups limit proton mobility and enable access to

146 tivity relationship investigation of various spacers guided by molecular modeling studies helped to i

147 and the blue InGaN quantum well, a thin GaN spacer has been introduced prior to the blue InGaN quant

148 d to the piperidine amine (i.e., lacking the spacer) have decreased binding affinity and residence ti

149 Sonication culture of cement spacers identifies a similar proportion of patients with

150 We investigated the fungal intergenic spacer (IGS) sequence in combination with nanopore seque

151 Beside acting as a physical spacer in between electrons and holes, interlayers shoul

152 define the role of antibiotics in the cement spacer in relation to reimplantation cultures and subseq

153 ent-controllable thickness of the dielectric spacer in the metamaterials.

154 PhiNM1, PhiNM4 and Phi12 can recombine with spacers in either chromosomal or plasmid-borne CRISPR lo

155 mportant for selection and processing of new spacers in naive adaptation, was also essential for prim

156 For TIRs and TDRs, GRF identifies spacers in the middle and mismatches/insertions or delet

157 Whether and how pre-existing spacers in type II systems affect the acquisition of new

158 inherited cytoplasmic markers (COI and rbcLS spacer) in the tissue of the hybrid.

159 n to crRNA is determined to be universal and spacer-independent for enhancing the sensitivity and spe

160 n of 2 mm spermidine quenched the off-target spacer insertion rate by a factor of 20-fold, and, in th

161 alone reverse transcriptase or its fusion to spacer integrase Cas1.

162 lays a potential role in the fidelity of the spacer integration also in vivo.

163 and Cas2 are directed to catalyze full-site spacer integration at specific DNA stretches where guani

164 over a 30 base pair DNA region for accurate spacer integration at the CRISPR repeat.

165 anism that ensures efficient and directional spacer integration in many CRISPR systems.

166 Here, we investigate spacer integration in vitro using proteins from Pyrococc

167 to coordinate directional and site-specific spacer integration into the CRISPR locus to ensure preci

168 Multiple spacer integration is a rare event which significance fo

169 put sequencing, we identified high frequency spacer integration occurring at the same CRISPR repeat b

170 hat purified Cas1 and Cas2 proteins catalyze spacer integration with high specificity for CRISPR repe

171 I (LAttice simulation engine for Sticker and Spacer Interactions) that enables the calculation of ful

172 Cas2 are sufficient to accurately integrate spacers into a minimal CRISPR locus.

173 Integration of spacers into CRISPR loci requires the Cas1/Cas2 integras

174 nce from the invader's genome, known as the 'spacer', into the CRISPR locus.

175 short segments of foreign genetic material (spacers) into the CRISPR locus.

176 he immunity provided by the initial acquired spacer is easily overcome by escaper phages.

177 s rely on sequencing of internal transcribed spacer (ITS) amplicons.

178 anger sequencing of the internal transcribed spacer (ITS) and D1/D2 regions of the ribosomal gene for

179 h great accuracy, while internal transcribed spacer (ITS) and D1/D2 sequencing were successfully empl

180 isolated, and a fungal internal transcribed spacer (ITS) clone library survey was performed on dust

181 alysis of the ribosomal internal transcribed spacer (ITS) from specimens obtained from humans, domest

182 umina sequencing of the internal transcribed spacer (ITS) gene.

183 ) rRNA genes and fungal Internal Transcribed Spacer (ITS) rDNA sequences, samples contained high dive

184 well as in the nuclear internal transcribed spacer (ITS) region and 28 S rRNA.

185 e-the nuclear ribosomal internal transcribed spacer (ITS) region-and offers all ~1 000 000 public fun

186 Importantly, the spacer keeps particles away from both the air-water inte

187 variants provides a continuous source of new spacers leading to high overall level of spacer acquisit

188 Increasing the spacer length beyond 6 nt destabilizes mRNA-tRNA-ribosom

189 rrelation between the functional potency and spacer length of the ligands was observed, an observatio

190 The dimeric capsular assemblies display the spacer-length-dependent self-sorting behavior in a four-

191 Cross-linking with reagents that differ in spacer lengths and targeting residues increases the spat

192 al sigma-factor regulons, along with various spacer lengths between the -10 and -35 elements.

193 s with the lipid backbone of an incorporated spacer lipid.

194 y by approximately 13-fold, with the highest spacer load present in the oral microbiome.

195 h numerous overlapping genes, highly reduced spacers, loss of all cis-spliced introns, and shrunken p

196 H(2))(2) (+); A' = ammonium cation acting as spacer; M = Ge(2+), Sn(2+), Pb(2+); and X = Cl(-), Br(-)

197 be 'naive', i.e. independent of any existing spacer matches, or it can be 'primed', i.e. spurred by t

198 o infect the more generalist hosts with many spacers matching many viruses).

199 MGE carrying type I-B CRISPR-Cas systems and spacers matching other thaumarchaeal iMGE, suggesting an

200 ost contains a CRISPR-Cas system with CRISPR spacers matching protospacers within the inverted duplic

201 the development of a numerical stickers-and-spacers model that enables predictions of full binodals

202 Careful design of the spacer moieties linking the dipolar chromophores within

203 Using an optimized ratio of PNA with a spacer molecule (MCH), the lowest limit of detection (Lo

204 inorganic layer number and length of organic spacer molecule affect stability.

205 CH(3)(CH(2))(5)NH(3)(+), C6) as the organic spacer molecules between the inorganic slabs, creating t

206 The increase in the length of the organic spacer molecules did not affect their optical properties

207 RC2 engages two nucleosomes separated by one spacer nucleosome, is a preferred low-energy configurati

208 lly reduce knockdown to a modest degree, but spacer nucleotides 15-21 are largely intolerant of targe

209 of the epitope, affinity and immobilization spacer of PBs as well as the influence of stop-flow dyna

210 RNA, D2-D3 of 28S rRNA, internal transcribed spacer of rRNA, mitochondrial cytochrome oxidase subunit

211 ized on the silicon, via two alkyl molecular spacers of different length.

212 was not influenced by the type of resin, the spacer, or the N-substituent.

213 Chemical tuning of spacer organic cations has attracted great interest due

214 r adaptation and reveal a mechanism by which spacer orientation is defined in E. coli.

215 ects the PAM sequence, which helps to define spacer orientation.

216 resulted in acquisition of large numbers of spacers originating from the entire genomic DNA.

217 turn led to preferred integration of single spacers over two transposon ends.

218 We observed that spacer patterns are established early during the CRISPR-

219 d Gag processing: the cleavage of the capsid-spacer peptide 1 (CA-SP1) intermediate to mature CA.

220 t that spans the junction between the CA and spacer peptide 1 (SP1) subunits of Gag, and interfere wi

221 ly conserved positions within the capsid and spacer peptide 1 domains of Gag that confer resistance.

222 e capsid (CA) domain of Gag and the flanking spacer peptide SP1 that stabilize VLPs, but much less is

223 Increasing its N-tail length with uncharged spacer peptides led to YidC dependence and eventually Yi

224 ad group and an antagonist-like oxypropylene spacer possess significant intrinsic activity for the ac

225 ere, we demonstrate that previously acquired spacers promote additional spacer acquisition from the v

226 ynamic functional droplets formed by sticker-spacer proteins.

227 he positional mismatch tolerance of observed spacer-protospacer pairs.

228 We amplified the internal transcribed spacer region 2 of the nuclear ribosomal operon to ident

229 sequencing) and fungal (internal transcribed spacer region amplicon sequencing) communities across re

230 ationship between the structure of the AdE1A spacer region and oncogenicity of HAdVs, the structures

231 The oncogenic spacer region is alpha-helical, which contrasts with pre

232 genicity related to the presence of a unique spacer region located between conserved regions 2 and 3

233 on the sequence of the internal transcribed spacer region of fungal ribosomal RNA encoding genes dem

234 ion between P54nrb and RNase H1 requires the spacer region of RNAse H1, while the P54nrb core domains

235 ering a hairpin secondary structure onto the spacer region of single guide RNAs (hp-sgRNAs) can incre

236 ng of the ribosomal RNA internal transcribed spacer region to examine the microdiversity within this

237 cal or very similar to the adenovirus 12 E1A spacer region were determined and found to be alpha-heli

238 ely, atpE genes and the internal transcribed spacer region.

239 uence, belonging to the Internal Transcribed Spacers region, was used to design the real-time PCR det

240 ions based on ribosomal internal transcribed spacer regions (ITS1/2) and expanded protein functional

241 ble predictive algorithms, the structures of spacer regions from other E1As were also examined, and t

242 for 5, we sequenced the internal transcribed spacer regions, and for 4 others the whole genomes.

243 Targeting by these virus-borne spacers represents a distinct mechanism of heterotypic s

244 antimicrobial suppression after destination-spacer retention did not significantly prevent reinfecti

245 Structures of TTCs containing longer spacers reveal a new state compatible with NusG bridging

246 somal assembly - the 5' external transcribed spacer ribonucleoprotein - provides a mechanism for how

247 the presence of the 5' external transcribed spacer RNA and all ribosomal RNA domains.

248 (D), thrombospondin-1 (T), Cys-rich (C), and spacer (S) domains (proximal domains), followed by 7 T a

249 (D), thrombospondin-1 (T), Cys-rich (C), and spacer (S) domains, followed by 7 additional T domains a

250 Chiral organic dyes comprising a donor (D), spacer (S), primary acceptor (A1), chiral bridge (B(S)),

251 Off-target integration of a new spacer sequence outside canonical CRISPR arrays has been

252 The 25 million CRISPR spacers sequenced from a single sampling site dwarf the

253 d and processed into individual CRISPR RNAs, spacer sequences guide Cas effector nucleases to destroy

254 rally defends against RNA phages(8), type VI spacer sequences have exclusively been found to match th

255 Our data demonstrate that spacer sequences not only specify the targets of Cas nuc

256 The taxonomy of spacer sequences parallels that of their source communit

257 ave very large genomes and since having more spacers should confer a better memory, it is puzzling th

258 Structures of TTCs containing short spacers show a state incompatible with NusG bridging and

259 d leader-side insertion before proceeding to spacer-side insertion.

260 and could perform independent leader-side or spacer-side insertions, although the leader-side inserti

261 bling leader-side integration and subsequent spacer-side integration.

262 gh the leader-side insertion was faster than spacer-side.

263 s, each containing a Zn(2+)-binding G5 and a spacer subdomain, is responsible for Zn(2+)-dependent as

264 CRATES allows assembly of repeat-spacer subunits using defined assembly junctions within

265 Furthermore, the PEG spacer successfully reduces nonspecific binding, enablin

266 r genetic and genomic evidence suggests that spacer-targeted elements preferentially associate with l

267 with spacer acquisition rates, but not with spacer targeting efficiency.

268 CRISPR spacer targeting indicates that Lak phages infect bacter

269 Cells with spacers targeting a subset of phage transcripts survived

270 This process is driven by foreign DNA spacer (termed protospacer) selection and integration me

271 orinated chain made of a propyl hydrogenated spacer terminated by a perfluorinated core of various le

272 93-111 in SNAP25 act as a flexible molecular spacer that ensures efficient coupling of the SNAP25-zDH

273 imR binds to an unusual operator with a long spacer that interacts nonspecifically with the receptor

274 y, in full-length Bs-FtsZ, the CTL acts as a spacer that spatially separates the CTP sticker from the

275 Surprisingly, many archaea possess spacers that match chromosomal genes of related species,

276 Spacers that protect the host from the phage demonstrate

277 g 2 naltrexone units through a rigid pyrrole spacer, the bivalent ligand norbinaltorphimine was forme

278 ERS (E = EC, R = Repeating sequence, and S = Spacer), these strategies can be easily integrated into

279 have a second nucleobase attached via amide spacer to a side chain at Cgamma on the repeating aeg un

280 t the crRNA-guided acquisition of additional spacers to achieve a more rapid and robust immunization

281 t are brought about by imperfect matching of spacers to the integrated phage sequences (prophages).

282 The effect of ethyne, ethene, and phenyl spacer units between the radical center and the incipien

283 vestigate DNA elements that control accurate spacer uptake in the type II-A CRISPR locus of Streptoco

284 in a metered-dose inhaler with or without a spacer, variability in peak expiratory flow of more than

285 Overall, a glycopeptide in the cement spacer was not associated with a lower failure rate (18%

286 were deleted, uptake of correctly processed spacers was observed, indicating that none of these inte

287 ver, the addition of a polyethylene glycol 8 spacer weakened affinity for albumin in each case.

288 In the absence of selection spacers were acquired from both strands of phage DNA, in

289 plantation, and cases without data on cement spacers were excluded.

290 te N,C-chelate organoboron dyes with alkynyl spacers were synthesized by Heck alkynylation.

291 Spacers were varied with respect to the core unit as wel

292 Immune memories are stored in the form of 'spacers' which are short DNA sequences that are captured

293 ribosomal RNA at the 5' external transcribed spacer, which directs the early association of assembly

294 ion depended on sequence elements within the spacer, which in turn determined the abundance of differ

295 sition of short viral DNA sequences known as spacers, which are transcribed into short RNA guides to

296 hat shape the distribution of newly acquired spacers, which is observed to be uneven, are poorly unde

297 on one receptor but having different peptide spacer with Avitag sequence necessary for their immobili

298 equence analysis of the internal transcribed spacer with or without D1/D2 ribosomal RNA regions.

299 or (D-A-D) systems based on a pyrimidine pai-spacer with various substituents at the C-2 position has

300 n turn determined the abundance of different spacers within the adapted population.