ライフサイエンスコーパス: spermatid

1 t germ cells unable to progress beyond round spermatid.

2 the activation signal is transduced into the spermatid.

3 zed within the two huge mitochondria of each spermatid.

4 a centriole-independent compartment in mouse spermatids.

5 g in a decreased number of spermatocytes and spermatids.

6 from the primary spermatocyte stage through spermatids.

7 al region of round, elongating and elongated spermatids.

8 he onset of MSCI and persists in postmeiotic spermatids.

9 marked decrease of the endogenous kinases in spermatids.

10 eiotic spermatocytes and postmeiotic haploid spermatids.

11 rom inactive sex chromosomes in post-meiotic spermatids.

12 ganization property of Brdt in haploid round spermatids.

13 colocalizes with acetylated H4 in elongating spermatids.

14 are unable to mature into spermatocytes and spermatids.

15 s silencing and is activated in post-meiotic spermatids.

16 t4d is highly expressed in spermatocytes and spermatids.

17 meiosis I and after exit from meiosis II, in spermatids.

18 is, and silencing persists into post-meiotic spermatids.

19 Dicer-null pachytene spermatocytes or round spermatids.

20 branous organelles (MOs) in undifferentiated spermatids.

21 control versus Brdt(DeltaBD1/DeltaBD1) round spermatids.

22 '-untranslated region (UTR) typical of round spermatids.

23 ished on the sex chromosomes in post-meiotic spermatids.

24 rise to bi-nucleated or even tetra-nucleated spermatids.

25 in investigating sex chromosome dynamics in spermatids.

26 BK-d results in loss of spermatozoa, but not spermatids.

27 and Mena in the subacrosomal layer of round spermatids.

28 ndergo chromatin reorganization in elongated spermatids.

29 s to repress sex chromosome transcription in spermatids.

30 ion and maintain steady expression levels in spermatids.

31 d primarily in testis, almost exclusively in spermatids.

32 SLY is found in the nucleus and cytoplasm of spermatids.

33 ern of ACROSIN staining as observed in human spermatids.

34 g to substantial spermidine synthesis in the spermatids.

35 are essential for chromatin condensation in spermatids.

36 n the X chromosome remain repressed in round spermatids.

37 ely created from mRNAs that are expressed in spermatids.

38 ospho-GRTH in the chromatoid bodies of round spermatids.

39 retrotransposon LINE1 de-repression in round spermatids.

40 erentiating spermatogonia, spermatocytes and spermatids.

41 ion sequesters RNF8 in the cytoplasm of late spermatids.

42 anscriptionally inactive state in late-stage spermatids.

43 NHE8 localizes to the developing acrosome of spermatids.

44 ls but was undetectable in spermatocytes and spermatids.

45 icing and localizes to giant mitochondria in spermatids.

46 of meiosis as noted by the presence of round spermatids.

47 Here we report that, in Drosophila spermatids, a conserved module of transition zone protei

48 dramatic Se enrichment specifically in late spermatids, a pattern that was not seen in any other ele

49 m and hypomorphic phenotypes being premature spermatid activation and numerous sperm cell defects.

50 appears to be involved in the regulation of spermatid activation during spermiogenesis, with the nul

51 group gene products transduce the signal for spermatid activation initiated by extracellular zinc in

52 d production by meiosis) and spermiogenesis (spermatid activation into actively motile spermatozoa).

53 product functions in the transduction of the spermatid activation signal.

54 ould perturb spermatogenesis, in particular, spermatid adhesion (i.e., inducing apical ES degeneratio

55 tly regulated in adult rat testis to control spermatid adhesion and sperm release at spermiation.

56 hat sFRP1 might be correlated with elongated spermatid adhesion conferred by the apical ES before spe

57 alizes to cytoplasmic structures of maturing spermatids affected in Kdm3a mutant mice, which in turn

58 tis, the CRB3 KD testes displayed defects in spermatid and phagosome transport, and also spermatid po

59 rdt protein was lost in Brdt(BD1/BD1) mutant spermatids and Brdt and Sirt1 overlapped around the chro

60 global overexpression of sex-linked genes in spermatids and either a distorted sex ratio in favor of

61 an absence of mature flagella on elongating spermatids and epididymal sperm.

62 on in the size of chromatoid bodies of round spermatids and germ cell apoptosis.

63 stochemistry also in embryonic gonads and in spermatids and granulosa cells of adult testes and ovari

64 FANCI was localized in spermatocytes and spermatids and in the nucleus of oocytes.

65 acrosomal formation, degenerative elongating spermatids and irregular head morphology in postmeiotic

66 The Bsg KO testis lacks elongated spermatids and mature spermatozoa, a phenotype similar t

67 concomitant with defects in the transport of spermatids and phagosomes and a disruption of cell adhes

68 that they appear in elongating to condensing spermatids and predominantly associated with the chromat

69 n all affected men was NOA, but occasionally spermatids and rarely a few spermatozoa in the semen wer

70 and Hnrnpk mRNAs were longer in mutant round spermatids and resulted in reduced protein levels.

71 testis and more specifically in postmeiotic spermatids and sperm cells.

72 e (GRC) is eliminated from somatic cells and spermatids and transmitted via oocytes only.

73 ADAD1, previously shown to localize to round spermatids, and ADAD2 had distinct localization patterns

74 ntially become spermatogonia, spermatocytes, spermatids, and eventually spermatozoa.

75 ntly expressed in round and early elongating spermatids, and Hipk4 knockout males are sterile, exhibi

76 apical ectoplasmic specialization, malformed spermatids, and marked hyperspermia.

77 luding spermatogonia, spermatocytes, haploid spermatids, and spermatozoa, which takes place in the ep

78 d spermatids, apoptosis of spermatocytes and spermatids, and the appearance of numerous round cells i

79 n RNPs from pachytene spermatocytes to round spermatids, and the enrichment of shorter 3' UTR mRNAs i

80 cal alterations during acrosome formation in spermatids, and were significantly different in connecti

81 Tnp1, Spem1, and Catpser3; depletion of late spermatids; and male infertility.

82 ES is restricted to the Sertoli-spermatid (apical ES) interface, as well as the Sertoli

83 chimeras included failure to form elongated spermatids, apoptosis of spermatocytes and spermatids, a

84 progressively repressed as nuclei of haploid spermatids are compacted through a dramatic chromatin re

85 A high proportion of delayed spermatids are eliminated via apoptosis, with a conseque

86 These translation defects in haploid round spermatids are likely indirect, as neither MAEL nor piRN

87 sh with semicystic spermatogenesis, in which spermatids are released into the seminiferous lobule lum

88 oward the apical compartment until elongated spermatids are released into the tubule lumen during spe

89 family of post-meiotic kinases expressed in spermatids, are critical to spermiogenesis, and are requ

90 Coincident with round spermatid arrest, we find reduced mRNA expression of tra

91 of BRD4 with acetylated H4 decreases in late spermatids as acetylated histones are removed from the c

92 s co-localized with SSTK in the cytoplasm of spermatids as they undergo restructuring and chromatin c

93 NA interference created XX animals that made spermatids as well as oocytes, but their spermatids coul

94 required for proper basal body function and spermatid axoneme formation.

95 m functional centrosomes, but centrioles and spermatid basal bodies are short in length.

96 entriolar protein that also localizes to the spermatid basal body.

97 homeodomain transcription factor 1 and round spermatid basic protein 1.

98 Their round spermatids bear "ghost" CBs, whose architecture is great

99 satellite repeats but not drive-insensitive spermatids bearing few or no Rsp satellite repeats.

100 e-to-protamine transition in drive-sensitive spermatids bearing many Rsp satellite repeats but not dr

101 Once spermatids become activated to spermatozoa, the reporter

102 y disappeared at the apical ES in misaligned spermatids before their depletion.

103 y move along the sperm tails, removing inter-spermatid bridges and most of the cytoplasm.

104 5 is discarded into the residual body during spermatid budding, but a small amount remains in budded

105 ype mice, and in the manchette of elongating spermatids, but in the Meig1 or Pacrg-deficient mice, SP

106 tants, persisting nucleoids are swept out of spermatids by a cellular remodeling process that trims a

107 the reorganization of hyperacetylated round spermatid chromatin.

108 In elongating spermatids, CnnT forms speckles on the giant mitochondri

109 lasm is incomplete in Ube2j1(-/-) elongating spermatids, compromising the release of mature elongate

110 CAPZA3 protein localization was altered in spermatids concurrent with altered localization of a uni

111 revealed BRD4 in a ring around the nuclei of spermatids containing hyperacetylated histones.

112 ade spermatids as well as oocytes, but their spermatids could not activate without the addition of ma

113 P90 and TSSKs in germ cells, a mouse primary spermatid culture model was developed and characterized.

114 cts the metabolic processes occurring in the spermatid cytoplasm but does not lead to a global pertur

115 show elevated aneuploidy in metaphase II and spermatid death.

116 centriole docking to the nucleus, leading to spermatid decapitation as a result of a failure to form

117 ermatogenic and Sertoli cells as well as the spermatid deformities.

118 gonia to clusters of 64 post-meiotic haploid spermatids, demonstrating that RCs are stable and open t

119 By comparing the development of sperm- and spermatid-derived frog embryos, we show that the program

120 The Rfx2-null round spermatids detached from the seminiferous tubules, formi

121 RTH in the structure of the chromatoid body, spermatid development and completion of spermatogenesis

122 ls (LCs) and germ cells and is essential for spermatid development and completion of spermatogenesis.

123 y role as a morphogenetic determinant during spermatid development in the water fern Marsilea vestita

124 mal in the absence of GLD2, but post-meiotic spermatid development rapidly becomes abnormal.

125 es an immune-privileged site for postmeiotic spermatid development to avoid the production of antibod

126 , which we show is functionally required for spermatid development.

127 However, round spermatids did not progress beyond step 6, revealing a n

128 he decrease in centrosomal components during spermatid differentiation (spermiogenesis).

129 nd inactive pools, results in abnormal round spermatid differentiation and impaired fertility.

130 lanogaster, many transcripts needed for late spermatid differentiation are synthesized in pre-meiotic

131 ubiquitin proteasome system are required for spermatid differentiation during spermiogenesis.

132 l regulators of meiosis, RNA turnover during spermatid differentiation, and germ cell-soma communicat

133 Salto has dynamic localization during spermatid differentiation, being progressively relocated

134 (SPDS) inhibitor, was added at the start of spermatid differentiation, the spermatid nuclei remained

135 ribed, including the prior report of Ance in spermatid differentiation, Wolbachia-induced sex-specifi

136 tin condensation, cell cycle progression and spermatid differentiation.

137 g the plasma membrane increase necessary for spermatid differentiation.

138 otic spermatocytes and postmeiotic arrest of spermatid differentiation.

139 cgrba-activated signaling cascade in haploid spermatids directs gene expression and the progression o

140 rved in spermatids, the Brdt(BD1/BD1) mutant spermatids do not undergo apoptosis (on either backgroun

141 n of cell adhesion most notably in elongated spermatids due to a loss of actin-bundling capability at

142 changes are accompanied by a loss of haploid spermatids due to impeded meiosis.

143 d from cell fragments that were discarded by spermatids during spermiation.

144 g accompanied with the transit of developing spermatids during spermiogenesis must be segregated from

145 cellular transformation, during which round spermatids elongate into chromatin-condensed spermatozoa

146 ctin (F-actin)-scaffolded acroplaxome during spermatid elongation and abnormal head morphologies in m

147 roteins (chromatin remodelers, essential for spermatid elongation and completion of spermatogenesis)

148 nslationally activated during the process of spermatid elongation and maturation.

149 Slx/Slxl1 deficiency leads to delay in spermatid elongation and sperm release.

150 e two postmeiotic transitions: initiation of spermatid elongation and spermatozoa release.

151 entiation, meiotic initiation, initiation of spermatid elongation, and release of spermatozoa.

152 ed extensive germ cell apoptosis and blocked spermatid elongation, resulting in severe oligozoospermi

153 ing and is also capable of inducing elongate spermatid exfoliation through its disruptive effects on

154 We find that asun spermatocytes and spermatids exhibit drastic reduction of perinuclear dyne

155 pporting Sertoli cells, we show that haploid spermatids express the homolog of the tetrapod LHCGR (Lh

156 convergent acquisition and amplification of spermatid-expressed gene families on the X and Y chromos

157 mosomes that multiple families of sex-linked spermatid-expressed genes are highly amplified in Mus mu

158 stinctive simultaneous amplification seen in spermatid-expressed genes.

159 expansion of ampliconic sequence containing spermatid-expressed genes.

160 These multi-nucleated spermatids fail to undergo normal differentiation, leadi

161 t activate to crawling spermatozoa, although spermatids from mutant males activate through a pathway

162 cell division and the production of haploid spermatids from the tetraploid primary spermatocytes via

163 In postmeiotic round spermatids, genomic compartmentalization increases and g

164 modulations alter 3'-UTR processing in round spermatids; importantly, the BD1 is essential for these

165 d their abrupt disappearance from developing spermatids in a process requiring the mitochondrial nucl

166 zation in cultured cells and is expressed in spermatids in mouse testes.

167 expressed predominantly in spermatocytes and spermatids in mouse, and are reduced in men with impaire

168 T2 correlated with chromatin condensation of spermatids in murine testes.

169 tively expressed mainly by spermatocytes and spermatids in seminiferous tubules of the testis.

170 rough the steps of meiosis to generate round spermatids in testes of rats treated with an acute dose

171 er plasticity to support the transport of 1) spermatids in the adluminal compartment and 2) preleptot

172 seminiferous epithelium, and lack of mature spermatids in the epididymis.

173 TB) must remain intact during the transit of spermatids in the seminiferous epithelium, which is asso

174 immunodetected bound to the Lhcgrba of free spermatids in the SLL, showing that circulating gonadotr

175 pressed in pachytene spermatocytes and round spermatids in the testis.

176 f elongating/elongated spermatids (step 8-19 spermatids) in the epithelium.

177 matocytes, as well as in round and elongated spermatids, in normal human testes.

178 severe disruption of the actin cones of the spermatid individualization complex.

179 male flies are sterile and exhibit complete spermatid individualization defects.

180 disrupts the final stage of spermatogenesis, spermatid individualization, and causes male sterility.

181 The associated process, termed spermatid individualization, is facilitated by the apopt

182 rile, which is due to defects in meiosis and spermatid individualization, phenotypes that are also ob

183 hat dmPTB expression is necessary for proper spermatid individualization, the terminal step necessary

184 the actin cones that mediate D. melanogaster spermatid individualization.

185 implicated in Drosophila male fertility and spermatid individualization.

186 males are sterile due to specific defects in spermatid individualization.

187 bs1 genetically interacts with pelota during spermatid individualization.

188 lto/CG13164, involved in nuclear shaping and spermatid individualization.

189 horing junction restructuring at the Sertoli-spermatid interface induced by adjudin which mimics junc

190 n-rich adherens junction at the Sertoli cell-spermatid interface) to coordinate cellular events acros

191 most notably at the apical ES at the Sertoli-spermatid interface, and expressed stage-specifically du

192 ES at the Sertoli cell-elongating/elongated spermatid interface, which is known as apical ES and pos

193 morphological differentiation of the haploid spermatid into spermatozoa.

194 ) fuse with the plasma membrane to transform spermatids into fertilization-competent spermatozoa.

195 ot disrupt meiosis or the differentiation of spermatids into mature sperm.

196 spermiogenesis, the differentiation of round spermatids into mature spermatozoa.

197 ically to regulate the conversion of sessile spermatids into motile spermatozoa, implicating PI(3,4,5

198 red for postmeiotic differentiation of round spermatids into sperm.

199 compromising the release of mature elongate spermatids into the lumen of the seminiferous tubule.

200 Development from round into elongated spermatids is abrogated in Tdrd6(-/-) mice.

201 ure in the nuclei of spermatocytes and round spermatids is essential for correct splicing and the pro

202 m2 mRNA in pachytene spermatocytes and round spermatids is essential for their timely translation at

203 herin, whereas at later stages, apoptosis of spermatids is observed.

204 In vitro incubation of flatfish spermatids isolated from the SLL with rLh specifically p

205 ions in transcription and histone removal in spermatids, it is unknown whether other BET family prote

206 racterized by depletion of spermatocytes and spermatids leading to oligoteratozoospermia or azoosperm

207 , disruption of the blood-testis barrier and spermatid loss.

208 wever, only punctate expression of the round spermatid marker SP-10 in the acrosomal granule of germ

209 During spermatid maturation into sperm, these genes lose H3K4me

210 dividualization complex that is required for spermatid maturation, thereby impairing spermatogenesis.

211 tin remodeling events that take place during spermatid maturation.

212 lved in germ stem/progenitor cells (CDKN2B), spermatid maturation/spermiogenesis (metalloproteinase a

213 dramatically in spermatogenous cells as the spermatids mature.

214 at the apical ES was shown to correlate with spermatid movement and proper spermatid orientation.

215 expressed in spermatogonia, spermatocytes or spermatids, neither spermatogenesis nor fertility were a

216 salto-deficient spermatids show coiled spermatid nuclei at late maturation stages and stalled i

217 ved that a prominent feature of the aberrant spermatid nuclei is a fragmented chromocenter, a structu

218 the start of spermatid differentiation, the spermatid nuclei remained round, centrin failed to local

219 ead and flagellar formation was disrupted in spermatids of MEIG1-deficient mice.

220 not changed in the few remaining elongating spermatids of Meig1-deficient mice.

221 to the manchette in the remaining elongating spermatids of Pacrg-deficient mice, indicating that PACR

222 s of Piwi-interacting small RNAs abundant in spermatids of the adult mouse testis.

223 In the elongating spermatids of wild-type mice, PACRG colocalizes with alp

224 Chromatoid bodies (CBs) are characteristic spermatid organelles, which were suggested to function i

225 Strikingly, spermatid orientation, alignment and release, as well as

226 correlate with spermatid movement and proper spermatid orientation.

227 NF45 are conserved in two related proteins, spermatid perinuclear RNA-binding protein (SPNR) and zin

228 own these polarity and PCP proteins modulate spermatid polarity and adhesion via their effects on mic

229 is in vivo was knockdown by RNAi, defects in spermatid polarity and adhesion, as well as spermatid tr

230 pithelial cycle, participating in conferring spermatid polarity and cell adhesion in the testis.

231 spermatid and phagosome transport, and also spermatid polarity due to a disruption of F-actin organi

232 seemed to normally undergo spermatogenesis (spermatid production by meiosis) and spermiogenesis (spe

233 ly abundant in spermatocytes and early round spermatids, regardless of the type of the genomic sequen

234 ion specifically in the germline rescued the spermatid-related phenotypes, suggesting a cell autonomo

235 to be capable of inducing BTB remodeling and spermatid release across the epithelium.

236 la melanogaster, individualization of sister spermatids requires the formation of specialized actin c

237 il to either progress to anaphase or attempt spermatid-residual body partitioning.

238 etached from the nucleus in asun postmeiotic spermatids, resulting in abnormalities later in spermato

239 mmunoprecipitation followed by sequencing in spermatids revealed enrichment of BRD4 and acetylated hi

240 distal and proximal centrioles), but insect spermatids seem to contain only one centriole, which fun

241 aux mutant spermatids show a deficit in formation of the plasma mem

242 salto-deficient spermatids show coiled spermatid nuclei at late maturati

243 matogenesis, SD induces dysfunction of SD(+) spermatids so that SD/SD(+) males sire almost exclusivel

244 Spermatid specific thioredoxin-3 (SPTRX3 or TXNDC8) is a

245 e approach, a mutation was identified in the spermatid-specific "capping protein (actin filament) mus

246 o avoid the production of antibodies against spermatid-specific antigens, many of which express trans

247 thus acquiring an active chromatin state and spermatid-specific expression.

248 ption in spermatocytes of an otherwise round spermatid-specific promoter.

249 male and female fertility, promotes haploid spermatid-specific transcription but has distinct roles

250 down-regulation of approximately 300 mostly spermatid-specific transcripts in testis, including near

251 ack sperm with spermatogenic arrest at round spermatid stage and loss of the cytoplasmic phospho-GRTH

252 a postmeiotic germ cell arrest at the round spermatid stage in the seminiferous tubules of the testi

253 tly expressed from the spermatocyte to round spermatid stage, coinciding with the widespread expressi

254 g most highly expressed in the haploid round spermatid stage.

255 sis, ranging from the pachytene to the round spermatid stage.

256 of JQ1 evident at the spermatocyte and round spermatid stages cause a complete and reversible contrac

257 mes at the spermatogonial, spermatocyte, and spermatid stages of spermatogenesis.

258 Bsg KO mice was arrested at the early round spermatid stages.

259 arrest of spermatogenesis at an early round spermatid step.

260 intains the polarity of elongating/elongated spermatids (step 8-19 spermatids) in the epithelium.

261 Spermatids subsequently give rise to spermatozoa in the

262 profile, elevated expression in post-meiotic spermatids, suggested proteins that could be involved in

263 uration of spermatogonia, spermatocytes, and spermatids suggests the existence of precise programs of

264 lar remodeling process that trims and shapes spermatid tails.

265 Bam downregulation in spermatocytes affected spermatid terminal differentiation and resulted in incre

266 ownregulation of which is crucial for proper spermatid terminal differentiation.

267 a unique structure at the base of elongating spermatid that directs formation of the flagella.

268 ine, we found mafr-1 null males have smaller spermatids that are less capable in competition for fert

269 group genes result in hermaphrodite-derived spermatids that cannot activate to crawling spermatozoa,

270 spermatogenesis gives rise to interconnected spermatids that differentiate and individualize into mat

271 Most animals have two centrioles in spermatids (the distal and proximal centrioles), but ins

272 re fragmented chromocenters were observed in spermatids, the Brdt(BD1/BD1) mutant spermatids do not u

273 In Drosophila spermatids, the giant mitochondria provide structural pl

274 bditis elegans as they activate from a round spermatid to a mature, crawling spermatozoon.

275 RTH-null mice (azoospermic due to failure of spermatids to elongate).

276 ption, even though it fails to support round spermatids to enter spermiogenesis.

277 atogenesis and a mutation that allowed these spermatids to self-activate.

278 teleosts, the differentiation of postmeiotic spermatids to spermatozoa (spermiogenesis) is thought to

279 ed piRNAs, lack complementary targets in the spermatid transcriptome.

280 ition of actin branching caused a failure of spermatid transit plus a loss of proper orientation in t

281 spermatid polarity and adhesion, as well as spermatid transport were noted mediated via changes in F

282 During spermiogenesis, spermatids undergo dramatic morphological changes includ

283 step in spermatogenesis, when the spherical spermatid undergoes wholesale reorganization to produce

284 It was also noted that the round spermatids underwent eventual degeneration with the form

285 ion levels of the sex chromosomes in haploid spermatids via regulation of postmeiotic sex chromatin (

286 In Lis-1 spermatids, we observed loss of attachments between the

287 ermiogenesis because no elongating/elongated spermatids were detected in any of the tubules examined.

288 atocytes, pachytene spermatocytes, and round spermatids were purified from enzymatically dispersed te

289 y in the absence of Boule, and haploid round spermatids were readily detected.

290 udding, but a small amount remains in budded spermatids where it localizes to MOs as a discrete dot.

291 dine made in the jacket cells moves into the spermatids, where it is involved in the unmasking of sto

292 ncentrated near the lumen side of elongating spermatids, where structural components of sperm are for

293 ver, TDP-43 remains at the promoter in round spermatids, which express acrv1 mRNA.

294 cific (H1fnt) protein in Brdt(BD1/BD1) round spermatids, which may be linked to the previously report

295 escued via blocking RNF8-MIWI interaction in spermatids with an RNF8-N peptide.

296 ppropriately during meiosis II, resulting in spermatids with disengaged centrioles.

297 stablished during meiosis is maintained into spermatids with the silent compartment postmeiotic sex c

298 Treatment of spermatids with the V-ATPase inhibitor bafilomycin block

299 gment, whereas infertile wpk males developed spermatids with very short flagella that did not extend

300 pachytene spermatocytes and early elongating spermatids without affecting H3K9me3 levels.